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Dairy microbiology

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https://www.readbyqxmd.com/read/28197144/detection-and-enumeration-of-spore-forming-bacteria-in-powdered-dairy-products
#1
REVIEW
Aoife J McHugh, Conor Feehily, Colin Hill, Paul D Cotter
With the abolition of milk quotas in the European Union in 2015, several member states including Ireland, Luxembourg, and Belgium have seen year on year bi-monthly milk deliveries to dairies increase by up to 35%. Milk production has also increased outside of Europe in the past number of years. Unsurprisingly, there has been a corresponding increased focus on the production of dried milk products for improved shelf life. These powders are used in a wide variety of products, including confectionery, infant formula, sports dietary supplements and supplements for health recovery...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28155071/sequential-application-of-waste-whey-as-a-medium-component-for-kluyveromyces-lactis-cultivation-and-a-co-feeder-for-lipase-immobilization-by-clea-method
#2
Aušra Veteikytė, Rimantas Šiekštelė, Bronius Tvaska, Inga Matijošytė
Currently, much attention is paid to technologies which can be drivers of the circular economy across different sectors, in particular, to develop technologies for utilization or reusability of biocompatible materials from industrial waste. One of such is the milk whey, which is a cheap biobased raw material, the disposal of which is a major problem for the dairy industry. Our proposed and investigated technology is based on a continuous exploitation of the whey combining microbiology and biotechnology. Primarily, whey was used as a nutrition source for the cultivation of Kluyveromyces lactis with the aim to produce the targeted biocatalyst-lipase...
February 2, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28139955/cattle-derived-salmonella-enterica-serovar-dublin-infections-in-red-foxes-vulpes-vulpes-in-tyrol-austria
#3
Walter Glawischnig, Judit Lazar, Alice Wallner, Christian Kornschober
Salmonella enterica serovar Dublin is endemic in the cattle population in some areas of the Austrian province Tyrol, and each year single dairy farms have experienced clinical infections. To ascertain if Tyrolean red foxes ( Vulpes vulpes ) act as a reservoir for Salmonella spp., we tested hepatic tissue and intestinal content from foxes hunted in the years 2015-2016 by using microbiological methods. In addition, we included several fox fecal samples collected on a mountain pasture near chamois carcasses in the investigation...
January 31, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28131570/relationship-between-milk-cathelicidin-abundance-and-microbiologic-culture-in-clinical-mastitis
#4
M F Addis, V Bronzo, G M G Puggioni, C Cacciotto, V Tedde, D Pagnozzi, C Locatelli, A Casula, G Curone, S Uzzau, P Moroni
The availability of reliable tools to enable the sensitive and specific detection of mastitis in dairy cows can assist in developing control strategies and promote the more rational use of antibiotics. We have developed a milk cathelicidin ELISA that shows high sensitivity and specificity for dairy cow mastitis, based on latent class analysis. In this study, we investigated the effect of microbial agents on cathelicidin abundance in the milk of cows with clinical mastitis. We subjected 535 quarter milk samples (435 from quarters showing signs of clinical mastitis and 100 from healthy quarters as a control) to milk cathelicidin ELISA, somatic cell count (SCC), and microbiologic culture...
January 25, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28094204/microbial-contamination-of-drinking-water-from-risky-tubewells-situated-in-different-hydrological-regions-of-bangladesh
#5
Nepal C Dey, Mahmood Parvez, Digbijoy Dey, Ratnajit Saha, Lucky Ghose, Milan K Barua, Akramul Islam, Mushtaque R Chowdhury
This study, conducted in 40 selected upazilas covering four hydrological regions of Bangladesh, aimed at determining the risk of selected shallow tubewells (depth<30m) used for drinking purpose (n=26,229). This was based on WHO's sanitary inspection guidelines and identifying the association of sanitary inspection indicators and risk scores with microbiological contamination of shallow tubewells. The main objective of the study was to observe the seasonal and regional differences of microbial contamination and finally reaching a conclusion about safe distance between tubewells and latrines by comparing the contamination of two tubewell categories (category-1: distance ≤10m from nearest latrine; n=80 and category 2: distances 11-20m from nearest latrine; n=80) in different geographical contexts...
December 29, 2016: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28092619/concentration-of-lactoferrin-and-immunoglobulin-g-in-cows-milk-in-relation-to-health-status-of-the-udder-lactation-and-season
#6
K Musayeva, A Sederevičius, R Želvytė, I Monkevičienė, D Beliavska-Aleksiejūnė, S Kerzienė
The aim of this study was to analyze an effect of udder health status, somatic cell count (SCC), stage and number of lactations, and different seasons on the concentration of lactoferrin (LF) and immunoglobulin G (IgG) in quarter milk samples (n=120) from crossbreed (Lithuanian Black-and-White & Holstein) dairy cows. Quarter health status was based on SCC and microbiological analysis. The highest mean value of LF and IgG were observed in quarters with subclinical mastitis 0.1 ± 0.02 mg/ml and 0.41 ± 0.06 mg/ml, respectively...
December 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27956766/enterohemorrhagic-escherichia-coli-o157-in-milk-and-dairy-products-from-libya-isolation-and-molecular-identification-by-partial-sequencing-of-16s-rdna
#7
Aboubaker M Garbaj, Enas M Awad, Salah M Azwai, Said K Abolghait, Hesham T Naas, Ashraf A Moawad, Fatim T Gammoudi, Ilaria Barbieri, Ibrahim M Eldaghayes
AIM: The aim of this work was to isolate and molecularly identify enterohemorrhagic Escherichia coli (EHEC) O157 in milk and dairy products in Libya, in addition; to clear the accuracy of cultural and biochemical identification as compared with molecular identification by partial sequencing of 16S rDNA for the existing isolates. MATERIALS AND METHODS: A total of 108 samples of raw milk (cow, she-camel, and goat) and locally made dairy products (fermented cow's milk, Maasora, Ricotta and ice cream) were collected from some regions (Janzour, Tripoli, Kremiya, Tajoura and Tobruk) in Libya...
November 2016: Veterinary World
https://www.readbyqxmd.com/read/27899431/monitoring-process-water-quality-using-near-infrared-spectroscopy-and-partial-least-squares-regression-with-prediction-uncertainty-estimation
#8
Peter B Skou, Thilo A Berg, Stina D Aunsbjerg, Dorrit Thaysen, Morten A Rasmussen, Frans van den Berg
Reuse of process water in dairy ingredient production-and food processing in general-opens the possibility for sustainable water regimes. Membrane filtration processes are an attractive source of process-water recovery since the technology is already utilized in the dairy industry and its use is expected to grow considerably. At Arla Foods Ingredients (AFI), permeate from a reverse osmosis polisher filtration unit is sought to be reused as process water, replacing the intake of potable water. However, as for all dairy and food producers, the process-water quality must be monitored continuously to ensure food safety...
November 29, 2016: Applied Spectroscopy
https://www.readbyqxmd.com/read/27898868/relationships-among-ensiling-nutritional-fermentative-microbiological-traits-and-contamination-in-corn-silages-addressed-with-partial-least-squares-regression
#9
A Gallo, D Bassi, R Esposito, M Moschini, P S Cocconcelli, F Masoero
The objective of this work was to reduce the predictor dimensionality and to develop a model able to forecast contamination in corn silages. A survey on 33 dairy farms was performed, and samples from core, lateral, and apical parts of the feed-out face of corn silage bunkers were analyzed for chemical, biological (digestible and indigestible NDF), fermentative (pH, ammonia nitrogen, lactic acid, VFA, and ethanol), and microbiological (yeasts and molds) traits. Corn silage samples were analyzed for cell and spore counts by adoption of a molecular DNA-based method...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27892875/the-zoonotic-potential-of-lactococcus-garvieae-an-overview-on-microbiology-epidemiology-virulence-factors-and-relationship-with-its-presence-in-foods
#10
REVIEW
Alicia Gibello, Fátima Galán-Sánchez, M Mar Blanco, Manuel Rodríguez-Iglesias, Lucas Domínguez, José F Fernández-Garayzábal
Lactococcus garvieae is a relevant worldwide fish pathogen affecting various farmed and wild marine and freshwater species. It has also been isolated from other animals, such as ruminants with subclinical mastitis and pigs with pneumonia. From the early 90s, L. garvieae has been associated with different human infections, mainly endocarditis. During the last five years, human infections by this bacterium appear to be increasing, likely due to the improvement in microbiological methods for bacterial identification and the alertness of this bacterium by physicians...
December 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27889140/genotyping-of-methicillin-resistant-staphylococcus-aureus-mrsa-isolated-from-milk-and-dairy-products-in-south-italy
#11
M G Basanisi, G La Bella, G Nobili, I Franconieri, G La Salandra
Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen emerging in hospitals as well as community and livestock. MRSA is a significant and costly public health concern because it may enter the human food chain and contaminate milk and dairy products causing foodborne illness. This study aimed to determine the occurrence and the characteristics of MRSA isolated from 3760 samples of milk and dairy products in a previous survey conducted in southern Italy during 2008-2014. Overall out of 484 S. aureus strains isolated, 40 (8...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27866041/study-of-the-bacterial-diversity-of-foods-pcr-dgge-versus-lh-pcr
#12
Cristiana Garofalo, Elena Bancalari, Vesna Milanović, Federica Cardinali, Andrea Osimani, Maria Luisa Savo Sardaro, Benedetta Bottari, Valentina Bernini, Lucia Aquilanti, Francesca Clementi, Erasmo Neviani, Monica Gatti
The present study compared two culture-independent methods, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) and length-heterogeneity polymerase chain reaction (LH-PCR), for their ability to reveal food bacterial microbiota. Total microbial DNA and RNA were extracted directly from fourteen fermented and unfermented foods, and domain A of the variable regions V1 and V2 of the 16S rRNA gene was analyzed through LH-PCR and PCR-DGGE. Finally, the outline of these analyses was compared with bacterial viable counts obtained after bacterial growth on suitable selective media...
February 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27828758/gentamicin-and-ciprofloxacin-resistant-enterobacteriaceae-in-cattle-farms-in-israel-risk-factors-for-carriage-and-the-effect-of-microbiological-methodology-on-the-measured-prevalence
#13
Amos Adler, Na'ama Sturlesi, Noga Fallach, Deniz Zilberman-Barzilai, Omar Hussein, Shlomo E Blum, Eyal Klement, Mitchell J Schwaber, Yehuda Carmeli
Our objectives were to establish a methodology for surveillance of ciprofloxacin-resistant Enterobacteriaceae and gentamicin-resistant Enterobacteriaceae (CPRE and GNRE, respectively) in cattle and to study the prevalence and risk factors for carriage of these bacteria in a national survey. This was a point prevalence study conducted from July to October 2013 in Israel. Stool samples were collected from 1,226 cows in 123 sections of 40 farms of all production types. The number of CPRE- and GNRE-positive cows was highest in quarantine stations and fattening farms and was lowest in pasture farms (p < 0...
November 9, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27822529/thermus-and-the-pink-discoloration-defect-in-cheese
#14
Lisa Quigley, Daniel J O'Sullivan, David Daly, Orla O'Sullivan, Zuzana Burdikova, Rostislav Vana, Tom P Beresford, R Paul Ross, Gerald F Fitzgerald, Paul L H McSweeney, Linda Giblin, Jeremiah J Sheehan, Paul D Cotter
A DNA sequencing-based strategy was applied to study the microbiology of Continental-type cheeses with a pink discoloration defect. The basis for this phenomenon has remained elusive, despite decades of research. The bacterial composition of cheese containing the defect was compared to that of control cheese using 16S rRNA gene and shotgun metagenomic sequencing as well as quantitative PCR (qPCR). Throughout, it was apparent that Thermus, a carotenoid-producing genus, was present at higher levels in defect-associated cheeses than in control cheeses...
May 2016: MSystems
https://www.readbyqxmd.com/read/27818092/the-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-ms-identification-versus-biochemical-tests-a-study-with-enterobacteria-from-a-dairy-cattle-environment
#15
Naiara Miranda Bento Rodrigues, Greiciane França Bronzato, Gabrielli Stefaninni Santiago, Larissa Alvarenga Batista Botelho, Beatriz Meurer Moreira, Irene da Silva Coelho, Miliane Moreira Soares de Souza, Shana de Mattos de Oliveira Coelho
Mastitis adversely affects milk production and in general cows do not regain their full production levels post recovery, leading to considerable economic losses. Moreover the percentage decrease in milk production depends on the specific pathogen that caused the infection and enterobacteria are responsible for this greater reduction. Phenotypic tests are among the currently available methods used worldwide to identify enterobacteria; however they tend to misdiagnose the species despite the multiple tests carried out...
January 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27816242/short-communication-investigation-into-mycobacterium-avium-ssp-paratuberculosis-in-pasteurized-milk-in-italy
#16
A Serraino, P Bonilauri, F Giacometti, M Ricchi, G Cammi, S Piva, V Zambrini, A Canever, N Arrigoni
This study investigated the presence of viable Mycobacterium avium ssp. paratuberculosis (MAP) in pasteurized milk produced by Italian industrial dairy plants to verify the prediction of a previously performed risk assessment. The study analyzed 160 one-liter bottles of pasteurized milk from 2 dairy plants located in 2 different regions. Traditional cultural protocols were applied to 500mL of pasteurized milk for each sample. The investigation focused also on the pasteurization parameters and data on the microbiological characteristics of raw milk (total bacterial count) and pasteurized milk (Enterobacteriaceae and Listeria monocytogenes)...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27800167/evaluation-of-calcium-cyanamide-addition-during-co-composting-of-manure-and-maize-straw-in-a-forced-aeration-static-pile-system
#17
Huasai Simujide, Chen Aorigele, Chun-Jie Wang, Tian-Hua Zhang, Bai Manda
BACKGROUND: Composting is one of the most environmentally friendly treatments to inactivate pathogenic organisms or reduce them to acceptable levels. However, even under thermal conditions, some pathogenic organisms such as E. coli could exist for a long time in composting. Such great persistence may increase the possibility of outbreaks of these organisms and further increase the environmental load. Calcium cyanamide (CaCN2) has recently been recognized to have the fungicidal effect on the pathogens of the soilborne diseases...
2016: Journal of Environmental Health Science & Engineering
https://www.readbyqxmd.com/read/27766441/cryptosporidiosis-risk-in-new-zealand-children-under-5-years-old-is-greatest-in-areas-with-high-dairy-cattle-densities
#18
Aparna Lal, Timothy Dobbins, Nasser Bagheri, Michael G Baker, Nigel P French, Simon Hales
The public health risks associated with dairy farming intensification are an emerging concern. We examine the association between dairy cattle density and cryptosporidiosis risk in children <5 years old in New Zealand from 1997 to 2008, a period of rapid intensification of the dairy industry. Multi-level Poisson regression was used to model reported cryptosporidiosis (N = 3869 cases) incidence in relation to dairy cattle densities across urban and rural areas separately, after controlling for microbiological quality of public drinking water supplies and neighbourhood socio-economic factors using the Census Area Unit of residence...
December 2016: EcoHealth
https://www.readbyqxmd.com/read/27765254/dairy-products-and-the-maillard-reaction-a-promising-future-for-extensive-food-characterization-by-integrated-proteomics-studies
#19
REVIEW
Simona Arena, Giovanni Renzone, Chiara D'Ambrosio, Anna Maria Salzano, Andrea Scaloni
Heating of milk and dairy products is done using various technological processes with the aim of preserving microbiological safety and extending shelf-life. These treatments result in chemical modifications in milk proteins, mainly generated as a result of the Maillard reaction. Recently, different bottom-up proteomic methods have been applied to characterize the nature of these structural changes and the modified amino acids in model protein systems and/or isolated components from thermally-treated milk samples...
March 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27743665/the-lactococcus-genus-as-a-potential-emerging-mastitis-pathogen-group-a-report-on-an-outbreak-investigation
#20
M X Rodrigues, S F Lima, C H Higgins, S G Canniatti-Brazaca, R C Bicalho
The bacterium Lactococcus lactis is widely used in food production and in medical applications, and is considered safe for human and animal use. However, studies have also linked Lactococcus bacteria to infection. For example, certain variants of Lactococcus species have been associated with bovine mastitis (e.g., Lactococcus lactis and Lactococcus garvieae). In this study, we investigated an outbreak of bovine mastitis thought to be associated with Lactococcus bacteria by using microbiological and molecular techniques...
December 2016: Journal of Dairy Science
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