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Keren Regev, Brian C Healy, Fariha Khalid, Anu Paul, Renxin Chu, Shahamat Tauhid, Subhash Tummala, Camilo Diaz-Cruz, Radhika Raheja, Maria A Mazzola, Felipe von Glehn, Pia Kivisakk, Sheena L Dupuy, Gloria Kim, Tanuja Chitnis, Howard L Weiner, Roopali Gandhi, Rohit Bakshi
Importance: MicroRNAs (miRNAs) are promising multiple sclerosis (MS) biomarkers. Establishing the association between miRNAs and magnetic resonance imaging (MRI) measures of disease severity will help define their significance and potential impact. Objective: To correlate circulating miRNAs in the serum of patients with MS to brain and spinal MRI. Design, Setting, and Participants: A cross-sectional study comparing serum miRNA samples with MRI metrics was conducted at a tertiary MS referral center...
March 1, 2017: JAMA Neurology
Nasim Samandari, Aashiq H Mirza, Lotte B Nielsen, Simranjeet Kaur, Philip Hougaard, Siri Fredheim, Henrik B Mortensen, Flemming Pociot
AIMS/HYPOTHESIS: We aimed to identify circulating microRNA (miRNA) that predicts clinical progression in a cohort of 123 children with new-onset type 1 diabetes mellitus. METHODS: Plasma samples were prospectively obtained at 1, 3, 6, 12 and 60 months after diagnosis from a subset of 40 children from the Danish Remission Phase Cohort, and profiled for miRNAs. At the same time points, meal-stimulated C-peptide and HbA1c levels were measured and insulin-dose adjusted HbA1c (IDAA1c) calculated...
February 2017: Diabetologia
Kristyna Hudcova, Martina Raudenska, Jaromir Gumulec, Hana Binkova, Zuzana Horakova, Rom Kostrica, Petr Babula, Vojtech Adam, Michal Masarik
Altered expression of microRNAs (miRNAs) has been shown in many types of malignancies including the head and neck squamous cell carcinoma (HNSCC). Although there are many new and innovative approaches in the treatment of HNSCC, a clear marker of this disease is still missing. Three candidate miRNAs (miR-29c-3p, miR-200b-5p and miR-375-3p) were studied in connection with HNSCC using quantitative real-time PCR expression levels in 42 tissue samples of HNSCC patients and histologically normal tumour-adjacent tissue samples of these patients...
September 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Ivan Jovanovic, Maja Zivkovic, Mirjana Kostic, Zoran Krstic, Tamara Djuric, Ivana Kolic, Dragan Alavantic, Aleksandra Stankovic
BACKGROUND: The genetic cause of most congenital anomalies of the kidney and urinary tract (CAKUT) cases remains unknown, therefore the novel approaches in searching for the common disease denominators are required. miRs regulate gene expression in humans and therefore have potentially therapeutic and biomarker properties. No studies thus far have attempted to explore the miRs in human CAKUT. We applied a new strategy to identify most specific miRs associated with CAKUT, in pediatric patients...
2016: Journal of Translational Medicine
Nurul-Syakima Ab Mutalib, Sri Noraima Othman, Azliana Mohamad Yusof, Shahrun Niza Abdullah Suhaimi, Rohaizak Muhammad, Rahman Jamal
Background. Papillary thyroid carcinoma (PTC) is the commonest thyroid malignancy originating from the follicle cells in the thyroid. Despite a good overall prognosis, certain high-risk cases as in those with lymph node metastasis (LNM) have progressive disease and poorer prognosis. MicroRNAs are a class of non-protein-coding, 19-24 nucleotides single-stranded RNAs which regulate gene expression and these molecules have been shown to play a role in LNM. The integrated analysis of miRNAs and gene expression profiles together with transcription factors (TFs) has been shown to improve the identification of functional miRNA-target gene-TF relationships, providing a more complete view of molecular events underlying metastasis process...
2016: PeerJ
Franz Zehentmayr, Cornelia Hauser-Kronberger, Barbara Zellinger, Falk Hlubek, Claudia Schuster, Ulrich Bodenhofer, Gerd Fastner, Heinz Deutschmann, Philipp Steininger, Roland Reitsamer, Thorsten Fischer, Felix Sedlmayer
BACKGROUND: A long-term analysis by the Early Breast Cancer Trialist Group (EBCTG) revealed a strong correlation between local control and cancer-specific mortality. MicroRNAs (miRs), short (20-25 nucleotides) non-coding RNAs, have been described as prognosticators and predictors for breast cancer in recent years. The aim of the current study was to identify miRs that can predict local control after breast conserving therapy (BCT) in early stage breast cancer. RESULTS: Clinical data of 46 early stage breast cancer patients with local relapse after BCT were selected from the institutional database...
2016: Clinical Epigenetics
Beata Kuśnierz-Cabala, Ewelina Nowak, Mateusz Sporek, Artur Kowalik, Marek Kuźniewski, Francisco J Enguita, Ewa Stępień
OBJECTIVES: Vascular dysfunction is a severe complication which can cause organ ischemia and damage during acute pancreatitis (AP). Laboratory assessment of AP is based on several routine parameters and does not reflect endothelial dysfunction or organ injury. Recently, small non-protein-coding RNAs (miRNAs) have been introduced to laboratory diagnostics as new biomarkers or predictive parameters. Candidate miRNAs (hsa-miR-16-5p, -103a-3p, -122-5p, -126-5p, -148a-5p, -216a-5p, -375, and -551b-5b) were selected to check their possible clinical application in stratification of patients with different AP severity...
July 2015: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
Kwang Hyuck Lee, Jong Kyun Lee, Dong Wook Choi, In-Gu Do, Insuk Sohn, Kee-Taek Jang, Sin-Ho Jung, Jin Seok Heo, Seong Ho Choi, Kyu Taek Lee
OBJECTIVES: The aim of this study was to investigate microRNAs related to postoperative prognosis of pancreatic cancer by applying microarray analysis to stored surgical specimens. METHODS: We retrospectively analyzed the relationship between microRNA expression and postoperative survival of pancreatic cancer patients in Samsung Medical Center. After the correlation of microRNA expression between paraffin-embedded and fresh-frozen specimens was confirmed, we applied NanoString nCounter system to formalin fixed paraffin-embedded surgical specimens between 1995 September and 2010 June...
July 2015: Pancreas
Anahita Shaer, Negar Azarpira, Akbar Vahdati, Mohammad Hosein Karimi, Mehrdad Shariati
This paper focuses on the development of renewable sources of isletreplacement tissue for the treatment of type I diabetes mellitus. Placental tissue-derived mesenchymal stem cells (MSCs) are a promising source for regenerative medicine due to their plasticity and easy availability. They have the potential to differentiate into insulin-producing cells. miR-375 is a micro RNA that is expressed in the pancreas and involved in islet development. Human placental decidua basalis MSCs (PDB-MSCs) were cultured from full-term human placenta...
September 2014: Cellular & Molecular Biology Letters
Song Ye, Liu Yang, Xinyi Zhao, Wei Song, Weilin Wang, Shusen Zheng
Altered expressions of microRNAs (miRNAs) are reported in pancreatic cancer and associate with cancer pathogenesis, apoptosis, and cell growth, thereby functioning as either tumor suppressors or oncogenes. However, the majority of studies focus on defining the regulatory functions of miRNAs, whereas few investigations are directed toward assessing how the miRNA themselves are transcriptionally regulated. In this study, integration of published multi-level expression data and bioinformatics computational approach was used to predict two regulation mechanisms: transcription factors (TF)-miRNA-mRNA regulation and long non-coding RNA(lncRNA)-miRNA-mRNA regulation...
December 2014: Cell Biochemistry and Biophysics
Anahita Shaer, Negar Azarpira, Mohammad Hosein Karimi
Diabetes mellitus is characterized by either the inability to produce insulin or insensitivity to insulin secreted by the body. Islet cell replacement is an effective approach for diabetes treatment; however, it is not sufficient for all the diabetic patients. MicroRNAs (miRNAs) are a class of small noncoding RNAs that play an important role in mediating a broad and expanding range of biological activities, such as pancreas development. The present study aimed to develop a protocol to efficiently differentiate human induced pluripotent stem (iPS) cells into islet-like cell clusters (ILCs) in vitro by using miR-186 and miR-375...
September 2014: Applied Biochemistry and Biotechnology
B Cao, T Ji, B Zhou, J Zou, G Q Jiao
MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides in length, which play important roles in regulating gene expression post-transcriptionally. Several computational methods and algorithms have been developed to predict miRNA targets. In this study, we described a method that can be used to integrate miRNA target prediction data from multiple sources and gene expression data to predict target genes of particular miRNAs. We used hsa-miR-375 as an example to test the feasibility of our method...
2013: Genetics and Molecular Research: GMR
Junko Hamamoto, Kenzo Soejima, Satoshi Yoda, Katsuhiko Naoki, Sohei Nakayama, Ryosuke Satomi, Hideki Terai, Shinnosuke Ikemura, Takashi Sato, Hiroyuki Yasuda, Yuichiro Hayashi, Michiie Sakamoto, Toru Takebayashi, Tomoko Betsuyaku
Recent advances in the treatment of non-small cell lung cancer (NSCLC) with new agents require accurate histological subtyping at diagnosis to avoid the higher risk of an adverse response and to obtain the maximum therapeutic response. However, interobserver variability, tumor heterogeneity and the degree of differentiation may affect the decision concerning a pathological diagnosis of NSCLC. Therefore, the aim of this study was to identify specific microRNAs (miRNAs) as standardized biomarkers with high sensitivity and specificity in order to distinguish between squamous cell carcinoma (SCC) and adenocarcinoma (AC)...
August 2013: Molecular Medicine Reports
Bertram Bleck, Gabriele Grunig, Amanda Chiu, Mengling Liu, Terry Gordon, Angeliki Kazeros, Joan Reibman
Air pollution contributes to acute exacerbations of asthma and the development of asthma in children and adults. Airway epithelial cells interface innate and adaptive immune responses, and have been proposed to regulate much of the response to pollutants. Thymic stromal lymphopoietin (TSLP) is a pivotal cytokine linking innate and Th2 adaptive immune disorders, and is upregulated by environmental pollutants, including ambient particulate matter (PM) and diesel exhaust particles (DEP). We show that DEP and ambient fine PM upregulate TSLP mRNA and human microRNA (hsa-miR)-375 in primary human bronchial epithelial cells (pHBEC)...
April 1, 2013: Journal of Immunology: Official Journal of the American Association of Immunologists
Chiara Villa, Elisa Ridolfi, Chiara Fenoglio, Laura Ghezzi, Roberto Vimercati, Francesca Clerici, Alessandra Marcone, Salvatore Gallone, Maria Serpente, Claudia Cantoni, Rossana Bonsi, Sara Cioffi, Stefano Cappa, Massimo Franceschi, Innocenzo Rainero, Claudio Mariani, Elio Scarpini, Daniela Galimberti
Altered gene expression occurs in central nervous system disorders, including Alzheimer's disease (AD). Transcription factor Sp1 (specificity protein 1) can regulate the expression of several AD-related proteins, including amyloid-β protein precursor and tau. Sp1 is regulated by oxidative stress, and Sp1 mRNA was found to be upregulated in AD cortex and hippocampus. The distribution of three single nucleotide polymorphisms (SNPs), including rs7300593, rs17695156, and rs12821290, covering 100% Sp1 genetic variability, has been determined in a population of 393 AD patients as compared with 412 controls...
2013: Journal of Alzheimer's Disease: JAD
Saskia M Wilting, Wina Verlaat, Annelieke Jaspers, Nour A Makazaji, Reuven Agami, Chris J L M Meijer, Peter J F Snijders, Renske D M Steenbergen
Deregulated expression of microRNAs (miRNAs) is common and biologically relevant in cervical carcinogenesis and appears only partly related to chromosomal changes. We recently identified 32 miRNAs showing decreased expression in high-grade cervical intraepithelial neoplasia (CIN) and carcinomas not associated with a chromosomal loss, 6 of which were located within a CpG island. This study aimed to investigate to what extent these miRNAs are subject to DNA methylation-mediated transcriptional repression in cervical carcinogenesis...
February 2013: Epigenetics: Official Journal of the DNA Methylation Society
Mariska Bierkens, Oscar Krijgsman, Saskia M Wilting, Leontien Bosch, Annelieke Jaspers, Gerrit A Meijer, Chris J L M Meijer, Peter J F Snijders, Bauke Ylstra, Renske D M Steenbergen
Cervical cancer results from persistent infection with high-risk human papillomavirus (hrHPV). Common genetic aberrations in cervical (pre)cancers encompass large genomic regions with numerous genes, hampering identification of driver genes. This study aimed to identify genes functionally involved in HPV-mediated transformation by analysis of focal aberrations (<3 Mb) in high-grade cervical intraepithelial neoplasia (hgCIN). Focal chromosomal aberrations were determined in high-resolution array comparative genomic hybridization data of 60 hgCIN...
January 2013: Genes, Chromosomes & Cancer
Luke A Selth, Scott Townley, Joanna L Gillis, Aleksandra M Ochnik, Krisna Murti, Robyn J Macfarlane, Kim N Chi, Villis R Marshall, Wayne D Tilley, Lisa M Butler
Circulating microRNAs (miRNAs) are emerging as useful non-invasive markers of disease. The objective of this study was to use a mouse model of prostate cancer as a tool to discover serum miRNAs that could be assessed in a clinical setting. Global miRNA profiling identified 46 miRNAs at significantly altered levels (p ≤ 0.05) in the serum of TRansgenic Adenocarcinoma of Mouse Prostate (TRAMP) mice with advanced prostate cancer compared to healthy controls. A subset of these miRNAs with known human homologues were validated in an independent cohort of mice and then measured in serum from men with metastatic castration-resistant prostate cancer (mCRPC; n = 25) or healthy men (n = 25)...
August 1, 2012: International Journal of Cancer. Journal International du Cancer
Orsi Giricz, Paul A Reynolds, Andrew Ramnauth, Christina Liu, Tao Wang, Lesley Stead, Geoffrey Childs, Thomas Rohan, Nella Shapiro, Susan Fineberg, Paraic A Kenny, Olivier Loudig
Invasive lobular carcinoma (ILC) of the breast, characterized by loss of E-cadherin expression, accounts for 5-15% of invasive breast cancers and it is believed to arise via a linear histological progression. Genomic studies have identified a clonal relationship between ILC and concurrent lobular carcinoma in situ (LCIS) lesions, suggesting that LCIS may be a precursor lesion. It has been shown that an LCIS diagnosis confers a 15-20% risk of progression to ILC over a lifetime. Currently no molecular test or markers can identify LCIS lesions likely to progress to ILC...
January 2012: Journal of Pathology
X Zhang, Z Yan, J Zhang, L Gong, W Li, J Cui, Y Liu, Z Gao, J Li, L Shen, Y Lu
BACKGROUND: Recurrence is a major factor leading to treatment failure and death in gastric cancer (GC) patients following surgical resection. Importantly, the prediction of recurrence is critical in improving clinical outcomes. We isolated a group of microRNAs (miRNAs) and evaluated their usefulness as prognostic markers for the recurrence of GC. PATIENTS AND METHODS: A total of 65 GC patients were selected for systematic analysis, 29 patients with recurrence and 36 patients without recurrence...
October 2011: Annals of Oncology: Official Journal of the European Society for Medical Oncology
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