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https://www.readbyqxmd.com/read/28879148/implementation-of-broad-screening-with-ebola-rapid-diagnostic-tests-in-for%C3%A3-cariah-guinea
#1
Frantz Jean Louis, Jennifer Y Huang, Yacouba K Nebie, Lamine Koivogui, Gayatri Jayaraman, Nadine Abiola, Amanda Vansteelandt, Mary C Worrel, Judith Shang, Louise B Murphy, David L Fitter, Barbara J Marston, Lise Martel
BACKGROUND: Laboratory-enhanced surveillance is critical for rapidly detecting the potential re-emergence of Ebola virus disease. Rapid diagnostic tests (RDT) for Ebola antigens could expand diagnostic capacity for Ebola virus disease. OBJECTIVES: The Guinean National Coordination for Ebola Response conducted a pilot implementation to determine the feasibility of broad screening of patients and corpses with the OraQuick(®) Ebola RDT. METHODS: The implementation team developed protocols and trained healthcare workers to screen patients and corpses in Forécariah prefecture, Guinea, from 15 October to 30 November 2015...
2017: African Journal of Laboratory Medicine
https://www.readbyqxmd.com/read/28861524/deep-sequencing-of-rna-from-blood-and-oral-swab-samples-reveals-the-presence-of-nucleic-acid-from-a-number-of-pathogens-in-patients-with-acute-ebola-virus-disease-and-is-consistent-with-bacterial-translocation-across-the-gut
#2
Miles W Carroll, Sam Haldenby, Natasha Y Rickett, Bernadett Pályi, Isabel Garcia-Dorival, Xuan Liu, Gary Barker, Joseph Akoi Bore, Fara Raymond Koundouno, E Diane Williamson, Thomas R Laws, Romy Kerber, Daouda Sissoko, Nóra Magyar, Antonino Di Caro, Mirella Biava, Tom E Fletcher, Armand Sprecher, Lisa F P Ng, Laurent Rénia, N'faly Magassouba, Stephan Günther, Roman Wölfel, Kilian Stoecker, David A Matthews, Julian A Hiscox
In this study, samples from the 2013-2016 West African Ebola virus outbreak from patients in Guinea with Ebola virus disease (EVD) were analyzed to discover and classify what other pathogens were present. Throat swabs were taken from deceased EVD patients, and peripheral blood samples were analyzed that had been taken from patients when they presented at the treatment center with acute illness. High-throughput RNA sequencing (RNA-seq) and bioinformatics were used to identify the potential microorganisms. This approach confirmed Ebola virus (EBOV) in all samples from patients diagnosed as acute positive for the virus by quantitative reverse transcription-PCR in deployed field laboratories...
July 2017: MSphere
https://www.readbyqxmd.com/read/28813542/the-african-cholera-surveillance-network-africhol-consortium-meeting-10-11-june-2015-lom%C3%A3-togo
#3
Aline Munier, Berthe-Marie Njanpop-Lafourcade, Delphine Sauvageot, Raymond B Mhlanga, Léonard Heyerdahl, Johara Nadri, Richard Wood, Issaka Ouedraogo, Alexandre Blake, Laurent Akilimali Mukelenge, Jean-Claude B Anné, Abiba Banla Kere, Lucienne Dempouo, Sakoba Keita, José P M Langa, Issa Makumbi, Elibariki R Mwakapeje, Ian J Njeru, Olubunmi E Ojo, Isaac Phiri, Lorenzo Pezzoli, Bradford D Gessner, Martin Mengel
The fifth annual meeting of the African cholera surveillance network (Africhol) took place on 10-11 June 2015 in Lomé, Togo. Together with international partners, representatives from the 11 member countries -Cameroon, Côte d'Ivoire, Democratic Republic of Congo, Guinea, Kenya, Mozambique, Nigeria, Tanzania, Togo, Uganda, Zimbabwe- and an invited country (Malawi) shared their experience. The meeting featured three sessions: i) cholera surveillance, prevention and control in participating countries, ii) cholera surveillance methodology, such as cholera mapping, cost-effectiveness studies and the issue of overlapping epidemics from different diseases, iii) cholera laboratory diagnostics tools and capacity building...
2017: BMC Proceedings
https://www.readbyqxmd.com/read/28791241/ebola-virus-disease-essential-clinical-knowledge
#4
REVIEW
Mahmoud Tawfik Khalafallah, Omar Ali Aboshady, Shaban Ahmed Moawed, Menna Said Ramadan
Since its initial outbreak in 1976, Ebola virus disease (EVD) has affected thousands of people, causing severe illness with high mortality rates. In the absence of a vaccine or effective specific treatment, as well as the lack of early diagnostic and detective methods, the EVD outbreak has generated a significant worldwide health concern. Insufficient health-care system resources, deficient infection control measures, and the shortage of appropriate personal protective equipment acted as amplifiers of the outbreak extension, especially in poorly resourced and unprepared communities...
July 2017: Avicenna Journal of Medicine
https://www.readbyqxmd.com/read/28732038/analysis-of-patient-data-from-laboratories-during-the-ebola-virus-disease-outbreak-in-liberia-april-2014-to-march-2015
#5
Yuki Furuse, Mosoka Fallah, Hitoshi Oshitani, Ling Kituyi, Nuha Mahmoud, Emmanuel Musa, Alex Gasasira, Tolbert Nyenswah, Bernice Dahn, Luke Bawo
An outbreak of Ebola virus disease (EVD) in Liberia began in March 2014 and ended in January 2016. Epidemiological information on the EVD cases was collected and managed nationally; however, collection and management of the data were challenging at the time because surveillance and reporting systems malfunctioned during the outbreak. EVD diagnostic laboratories, however, were able to register basic demographic and clinical information of patients more systematically. Here we present data on 16,370 laboratory samples that were tested between April 4, 2014 and March 29, 2015...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28726603/clinical-laboratory-values-as-early-indicators-of-ebola-virus-infection-in-nonhuman-primates
#6
Ronald B Reisler, Chenggang Yu, Michael J Donofrio, Travis K Warren, Jay B Wells, Kelly S Stuthman, Nicole L Garza, Sean A Vantongeren, Ginger C Donnelly, Christopher D Kane, Mark G Kortepeter, Sina Bavari, Anthony P Cardile
The Ebola virus (EBOV) outbreak in West Africa during 2013-2016 demonstrated the need to improve Ebola virus disease (EVD) diagnostics and standards of care. This retrospective study compared laboratory values and clinical features of 3 nonhuman primate models of lethal EVD to assess associations with improved survival time. In addition, the study identified laboratory values useful as predictors of survival, surrogates for EBOV viral loads, and triggers for initiation of therapeutic interventions in these nonhuman primate models...
August 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28665950/mortality-among-pcr-negative-admitted-ebola-suspects-during-the-2014-15-outbreak-in-conakry-guinea-a-retrospective-cohort-study
#7
Brecht Ingelbeen, Elhadj Ibrahima Bah, Tom Decroo, Idrissa Balde, Helena Nordenstedt, Johan van Griensven, Anja De Weggheleire
Non-cases are suspect Ebola Virus Disease (EVD) cases testing negative by EVD RT-PCR after admission to an Ebola Treatment Centre (ETC). Admitting non-cases to an ETC prompts concerns on case- and workload in the ETC, risk for nosocomial EVD infection, and delays in diagnosis and disease-specific treatment. We retrospectively analysed characteristics, outcomes and determinants of death of EVD cases and non-cases admitted to the Conakry ETC in Guinea between 03/2014 and 09/2015. Of the 2362 admitted suspects who underwent full confirmatory PCR testing, 1540 (65...
2017: PloS One
https://www.readbyqxmd.com/read/28628619/south-african-ebola-diagnostic-response-in-sierra-leone-a-modular-high-biosafety-field-laboratory
#8
Janusz T Paweska, Petrus Jansen van Vuren, Gunther H Meier, Chantel le Roux, Ousman S Conteh, Alan Kemp, Cardia Fourie, Prabha Naidoo, Serisha Naicker, Phumza Ohaebosim, Nadia Storm, Orienka Hellferscee, Lisa K Ming Sun, Busisiwe Mogodi, Nishi Prabdial-Sing, Desiree du Plessis, Deidre Greyling, Shayne Loubser, Mark Goosen, Stewart D McCulloch, Terence P Scott, Alexandra Moerdyk, Wesley Dlamini, Kelfala Konneh, Idrissa L Kamara, Dauda Sowa, Samuel Sorie, Brima Kargbo, Shabir A Madhi
BACKGROUND: In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. METHODS AND FINDINGS: The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28588973/responding-to-ever-changing-epidemiological-dynamics-of-ebola-virus-disease
#9
Yuki Maehira, Yohei Kurosaki, Tomoya Saito, Jiro Yasuda, Masayoshi Tarui, Denis J M Malvy, Tsutomu Takeuchi
With the incidence and mortality rates of Ebola virus disease (EVD) in Guinea, Liberia and Sierra Leone now at zero and reports of the largest and most complex EVD outbreak in history no longer on the front pages of newspapers worldwide, the urgency of that crisis seems to have subsided. During this lull after the storm and before the next one, the international community needs to engage in a 'lessons-learned' exercise with respect to our collective scientific, clinical and public health preparedness. This engagement must identify pragmatic, innovative mechanisms at multinational, national and community levels that allow research and development of next generation diagnostics and therapeutics, the safe and effective practice of medicine, and the maintenance of public health to keep pace with the rapid epidemiological dynamics of EVD and other deadly infectious diseases...
November 2016: BMJ Global Health
https://www.readbyqxmd.com/read/28577861/diagnostic-preparedness-for-infectious-disease-outbreaks
#10
Mark D Perkins, Christopher Dye, Manica Balasegaram, Christian Bréchot, Jean-Vivien Mombouli, John-Arne Røttingen, Marcel Tanner, Catharina C Boehme
Diagnostics are crucial in mitigating the effect of disease outbreaks. Because diagnostic development and validation are time consuming, they should be carried out in anticipation of epidemics rather than in response to them. The diagnostic response to the 2014-15 Ebola epidemic, although ultimately effective, was slow and expensive. If a focused mechanism had existed with the technical and financial resources to drive its development ahead of the outbreak, point-of-care Ebola tests supporting a less costly and more mobile response could have been available early on in the diagnosis process...
May 31, 2017: Lancet
https://www.readbyqxmd.com/read/28573636/ebola-virus-field-sample-collection
#11
Brian R Amman, Amy J Schuh, Jonathan S Towner
Sampling wildlife for ebolaviruses presents the researcher with a multitude of challenges, foremost of which is safety. Throughout the methods described in this chapter, personal safety and personal protective equipment (PPE) will be reiterated for each methodology. The methods described here are those used to successfully detect and isolate marburgviruses from their natural reservoir, Rousettus aegyptiacus, and therefore should be applicable for diagnostic testing for ebolaviruses via RT-PCR, ELISA, and IHC techniques...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28573633/real-time-and-end-point-pcr-diagnostics-for-ebola-virus
#12
Allen Grolla
Reverse transcriptase polymerase chain reaction (RT-PCR)-based techniques allow for highly sensitive and specific detection of RNA viruses. Detection of the amplification products can be achieved using several methods. The following are descriptions of the detection of ebolavirus RNA using end-point RT-PCR (agarose gel visualization of amplification products) and quantitative RT-PCR (Q-RT-PCR), with fluorescent detection using an intercalating dye or detection with the use of 5' hydrolysis probe assays. All of these techniques can be used to accurately detect the presence of ebolavirus in samples...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28573619/generation-of-recombinant-ebola-viruses-using-reverse-genetics
#13
Allison Groseth
Reverse genetics systems encompass a wide array of tools aimed at recapitulating some or all of the virus life cycle. In their most complete form, full-length clone systems allow us to use plasmid-encoded versions of the ribonucleoprotein (RNP) components to initiate the transcription and replication of a plasmid-encoded version of the complete viral genome, thereby initiating the complete virus life cycle and resulting in infectious virus. As such this approach is ideal for the generation of tailor-made recombinant filoviruses, which can be used to study virus biology...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28520958/development-use-and-impact-of-a-global-laboratory-database-during-the-2014-ebola-outbreak-in-west-africa
#14
Kara N Durski, Shalini Singaravelu, Junxiong Teo, Dhamari Naidoo, Luke Bawo, Amara Jambai, Sakoba Keita, Ali Ahmed Yahaya, Beatrice Muraguri, Brice Ahounou, Victoria Katawera, Fredson Kuti-George, Yacouba Nebie, T Henry Kohar, Patrick Jowlehpah Hardy, Mamoudou Harouna Djingarey, David Kargbo, Nuha Mahmoud, Yewondwossen Assefa, Orla Condell, Magassouba N'Faly, Leon Van Gurp, Margaret Lamanu, Julia Ryan, Boubacar Diallo, Foday Daffae, Dikena Jackson, Fayyaz Ahmed Malik, Philomena Raftery, Pierre Formenty
Background: The international impact, rapid widespread transmission, and reporting delays during the 2014 Ebola outbreak in West Africa highlighted the need for a global, centralized database to inform outbreak response. The World Health Organization and Emerging and Dangerous Pathogens Laboratory Network addressed this need by supporting the development of a global laboratory database. Methods: Specimens were collected in the affected countries from patients and dead bodies meeting the case definitions for Ebola virus disease...
June 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28505570/clinical-evaluation-of-the-biofire-filmarray-%C3%A2-biothreat-e-test-for-the-diagnosis-of-ebola-virus-disease-in-guinea
#15
Françoise Gay-Andrieu, N'Fally Magassouba, Valentina Picot, Cynthia L Phillips, Christophe N Peyrefitte, Brigitte Dacosta, Ahmadou Doré, Fode Kourouma, Véronique Ligeon-Ligeonnet, Corentin Gauby, Christophe Longuet, Matt Scullion, Ousmane Faye, Jean Louis Machuron, Mark Miller
BACKGROUND: The recent West Africa Ebola outbreak highlighted the need to provide access to rapid, safe and reliable Ebola Virus Disease diagnostics. OBJECTIVES: The objective of this field study was to assess the clinical performance of the FilmArray(®) BioThreat-E test for the detection of Ebola Zaïre virus in whole blood in symptomatic patients suspected of Ebola Virus Disease in Conakry (Guinea) from March to July 2015. STUDY DESIGN: The BioThreat-E test was compared to the two RT-PCRs, using serum, implemented at Donka Hospital in the emergency context: an in-house developed quantitative one-step RT-PCR adapted from the Weidmann technique, and the RealStar(®) Filovirus RT-PCR Kit 1...
July 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28505171/rapid-deployment-of-a-mobile-biosafety-level-3-laboratory-in-sierra-leone-during-the-2014-ebola-virus-epidemic
#16
Yi Zhang, Yan Gong, Chengyu Wang, Wensen Liu, Zhongyi Wang, Zhiping Xia, Zhaoyang Bu, Huijun Lu, Yang Sun, Xiaoguang Zhang, Yuxi Cao, Fan Yang, Haoxiang Su, Yi Hu, Yongqiang Deng, Bo Zhou, Zongzheng Zhao, Yingying Fu, David Kargbo, Foday Dafae, Brima Kargbo, Alex Kanu, Linna Liu, Jun Qian, Zhendong Guo
BACKGROUND: Ebola virus emerged in West Africa in December 2013. The high population mobility and poor public health infrastructure in this region led to the development of the largest Ebola virus disease (EVD) outbreak to date. METHODOLOGY/PRINCIPAL FINDINGS: On September 26, 2014, China dispatched a Mobile Biosafety Level-3 Laboratory (MBSL-3 Lab) and a well-trained diagnostic team to Sierra Leone to assist in EVD diagnosis using quantitative real-time PCR, which allowed the diagnosis of suspected EVD cases in less than 4 hours from the time of sample receiving...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28465576/paper-based-rna-detection-and-multiplexed-analysis-for-ebola-virus-diagnostics
#17
Laura Magro, Béatrice Jacquelin, Camille Escadafal, Pierre Garneret, Aurélia Kwasiborski, Jean-Claude Manuguerra, Fabrice Monti, Anavaj Sakuntabhai, Jessica Vanhomwegen, Pierre Lafaye, Patrick Tabeling
The most performing techniques enabling early diagnosis of infectious diseases rely on nucleic acid detection. Today, because of their high technicality and cost, nucleic acid amplification tests (NAAT) are of benefit only to a small fraction of developing countries population. By reducing costs, simplifying procedures and enabling multiplexing, paper microfluidics has the potential to considerably facilitate their accessibility. However, most of the studies performed in this area have not quit the lab. This letter brings NAAT on paper closer to the field, by using clinical samples and operating in a resource-limited setting...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28459810/external-quality-assessment-study-for-ebolavirus-pcr-diagnostic-promotes-international-preparedness-during-the-2014-2016-ebola-outbreak-in-west-africa
#18
MULTICENTER STUDY
Heinz Ellerbrok, Sonja Jacobsen, Pranav Patel, Toni Rieger, Markus Eickmann, Stephan Becker, Stephan Günther, Dhamari Naidoo, Livia Schrick, Kathrin Keeren, Angelina Targosz, Anette Teichmann, Pierre Formenty, Matthias Niedrig
During the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global preparedness for Ebola diagnostics. Detection of viral RNA by reverse transcription polymerase chain reaction has proven effective for diagnosis of ebolavirus disease and several assays are available. However, reliability of these assays is largely unknown and requires serious evaluation...
May 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28457350/ebola-virus-disease-an-update-on-epidemiology-symptoms-laboratory-findings-diagnostic-issues-and-infection-prevention-and-control-issues-for-laboratory-professionals
#19
REVIEW
Colin S Brown, Stephen Mepham, Robert J Shorten
The 2014 to 2016 Ebola virus disease (EVD), through the sheer size of the outbreak and combined experience within both resource-rich and resource-poor settings, allowed for more information to be gained about the clinical and pathologic features of EVD. This review highlights the range of aspects of EVD that the authors find are relevant to laboratory medicine, including the need for robust prediagnostic and laboratory processing algorithms to inform sampling of suspect patients, the vast majority of whom, in resource-rich settings, will have another diagnosis...
June 2017: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/28440096/strategies-in-ebola-virus-disease-evd-diagnostics-at-the-point-of-care
#20
Chad T Coarsey, Nwadiuto Esiobu, Ramswamy Narayanan, Mirjana Pavlovic, Hadi Shafiee, Waseem Asghar
Ebola virus disease (EVD) is a devastating, highly infectious illness with a high mortality rate. The disease is endemic to regions of Central and West Africa, where there is limited laboratory infrastructure and trained staff. The recent 2014 West African EVD outbreak has been unprecedented in case numbers and fatalities, and has proven that such regional outbreaks can become a potential threat to global public health, as it became the source for the subsequent transmission events in Spain and the USA. The urgent need for rapid and affordable means of detecting Ebola is crucial to control the spread of EVD and prevent devastating fatalities...
April 25, 2017: Critical Reviews in Microbiology
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