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https://www.readbyqxmd.com/read/29473349/benchtop-and-animal-validation-of-a-portable-fluorescence-microscopic-imaging-system-for-potential-use-in-cholecystectomy
#1
Jian Ye, Guanghui Liu, Peng Liu, Shiwu Zhang, Pengfei Shao, Zachary J Smith, Chenhai Liu, Ronald X Xu
We propose a portable fluorescence microscopic imaging system (PFMS) for intraoperative display of biliary structure and prevention of iatrogenic injuries during cholecystectomy. The system consists of a light source module, a camera module, and a Raspberry Pi computer with an LCD. Indocyanine green (ICG) is used as a fluorescent contrast agent for experimental validation of the system. Fluorescence intensities of the ICG aqueous solution at different concentration levels are acquired by our PFMS and compared with those of a commercial Xenogen IVIS system...
February 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29457703/exploration-of-assistive-technology-for-uniform-laparoscopic-surgery
#2
Masakazu Sato, Minako Koizumi, Takahiro Hino, Yu Takahashi, Natsuki Nagashima, Nao Itaoka, Chiharu Ueshima, Maki Nakata, Yoko Hasumi
INTRODUCTION: Laparoscopic surgery is less invasive than open surgery and is now common in various medical fields. However, laparoscopic surgery is more difficult than open surgery and often requires additional time for the operator to achieve mastery. Therefore, we investigated the use of assistive technology for uniform laparoscopic surgery. METHODS: We used the OpenCV2 library for augmented reality with an ArUco marker to detect and estimate forceps positioning...
February 19, 2018: Asian Journal of Endoscopic Surgery
https://www.readbyqxmd.com/read/29439398/a-real-time-wireless-sweat-rate-measurement-system-for-physical-activity-monitoring
#3
Andrew Brueck, Tashfin Iftekhar, Alicja B Stannard, Kumar Yelamarthi, Tolga Kaya
There has been significant research on the physiology of sweat in the past decade, with one of the main interests being the development of a real-time hydration monitor that utilizes sweat. The contents of sweat have been known for decades; sweat provides significant information on the physiological condition of the human body. However, it is important to know the sweat rate as well, as sweat rate alters the concentration of the sweat constituents, and ultimately affects the accuracy of hydration detection...
February 10, 2018: Sensors
https://www.readbyqxmd.com/read/29415450/design-and-implementation-of-cloud-centric-configuration-repository-for-diy-iot-applications
#4
Shabir Ahmad, Lei Hang, Do Hyeun Kim
The Do-It-Yourself (DIY) vision for the design of a smart and customizable IoT application demands the involvement of the general public in its development process. The general public lacks the technical knowledge for programming state-of-the-art prototyping and development kits. The latest IoT kits, for example, Raspberry Pi, are revolutionizing the DIY paradigm for IoT, and more than ever, a DIY intuitive programming interface is required to enable the masses to interact with and customize the behavior of remote IoT devices on the Internet...
February 6, 2018: Sensors
https://www.readbyqxmd.com/read/29414075/cost-effective-flow-through-nanohole-array-based-biosensing-platform-for-the-label-free-detection-of-uropathogenic-e-coli-in-real-time
#5
Juan Gomez-Cruz, Srijit Nair, Angel Manjarrez-Hernandez, Sandra Gavilanes-Parra, Gabriel Ascanio, Carlos Escobedo
Rapid, inexpensive and sensitive detection of uropathogenic Escherichia coli (UPEC), a common cause of ascending urinary tract infections (UTIs) including cystitis and pyelonephritis, is critical given the increasing number of cases and its recurrence worldwide. In this paper, we present a label-free nanoplasmonic sensing platform, built with off-the-shelf optical and electronic components, which can detect intact UPEC at concentrations lower than the physiological limit for UTI diagnosis, in real time. The sensing platform consists of a red LED light source, lens assembly, CMOS detector, Raspberry Pi interface in conjugation with a metallic flow-through nanohole array-based sensor...
January 31, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29390658/design-of-a-cathodoluminescence-image-generator-using-a-raspberry-pi-coupled-to-a-scanning-electron-microscope
#6
Alfredo Benítez, Ulises Santiago, John E Sanchez, Arturo Ponce
In this work, an innovative cathodoluminescence (CL) system is coupled to a scanning electron microscope and synchronized with a Raspberry Pi computer integrated with an innovative processing signal. The post-processing signal is based on a Python algorithm that correlates the CL and secondary electron (SE) images with a precise dwell time correction. For CL imaging, the emission signal is collected through an optical fiber and transduced to an electrical signal via a photomultiplier tube (PMT). CL Images are registered in a panchromatic mode and can be filtered using a monochromator connected between the optical fiber and the PMT to produce monochromatic CL images...
January 2018: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29283371/a-distributed-wireless-camera-system-for-the-management-of-parking-spaces
#7
Stanislav Vítek, Petr Melničuk
The importance of detection of parking space availability is still growing, particularly in major cities. This paper deals with the design of a distributed wireless camera system for the management of parking spaces, which can determine occupancy of the parking space based on the information from multiple cameras. The proposed system uses small camera modules based on Raspberry Pi Zero and computationally efficient algorithm for the occupancy detection based on the histogram of oriented gradients (HOG) feature descriptor and support vector machine (SVM) classifier...
December 28, 2017: Sensors
https://www.readbyqxmd.com/read/29165397/low-computational-cost-footprint-deformities-diagnosis-sensor-through-angles-dimensions-analysis-and-image-processing-techniques
#8
J Rodolfo Maestre-Rendon, Tomas A Rivera-Roman, Juan M Sierra-Hernandez, Ivan Cruz-Aceves, Luis M Contreras-Medina, Carlos Duarte-Galvan, Arturo A Fernandez-Jaramillo
Manual measurements of foot anthropometry can lead to errors since this task involves the experience of the specialist who performs them, resulting in different subjective measures from the same footprint. Moreover, some of the diagnoses that are given to classify a footprint deformity are based on a qualitative interpretation by the physician; there is no quantitative interpretation of the footprint. The importance of providing a correct and accurate diagnosis lies in the need to ensure that an appropriate treatment is provided for the improvement of the patient without risking his or her health...
November 22, 2017: Sensors
https://www.readbyqxmd.com/read/29151844/pym-a-new-affordable-image-based-method-using-a-raspberry-pi-to-phenotype-plant-leaf-area-in-a-wide-diversity-of-environments
#9
Benoît Valle, Thierry Simonneau, Romain Boulord, Francis Sourd, Thibault Frisson, Maxime Ryckewaert, Philippe Hamard, Nicolas Brichet, Myriam Dauzat, Angélique Christophe
Background: Plant science uses increasing amounts of phenotypic data to unravel the complex interactions between biological systems and their variable environments. Originally, phenotyping approaches were limited by manual, often destructive operations, causing large errors. Plant imaging emerged as a viable alternative allowing non-invasive and automated data acquisition. Several procedures based on image analysis were developed to monitor leaf growth as a major phenotyping target. However, in most proposals, a time-consuming parameterization of the analysis pipeline is required to handle variable conditions between images, particularly in the field due to unstable light and interferences with soil surface or weeds...
2017: Plant Methods
https://www.readbyqxmd.com/read/29151842/a-do-it-yourself-phenotyping-system-measuring-growth-and-morphology-throughout-the-diel-cycle-in-rosette-shaped-plants
#10
Andrei Dobrescu, Livia C T Scorza, Sotirios A Tsaftaris, Alistair J McCormick
Background: Improvements in high-throughput phenotyping technologies are rapidly expanding the scope and capacity of plant biology studies to measure growth traits. Nevertheless, the costs of commercial phenotyping equipment and infrastructure remain prohibitively expensive for wide-scale uptake, while academic solutions can require significant local expertise. Here we present a low-cost methodology for plant biologists to build their own phenotyping system for quantifying growth rates and phenotypic characteristics of Arabidopsis thaliana rosettes throughout the diel cycle...
2017: Plant Methods
https://www.readbyqxmd.com/read/29140977/low-cost-and-open-source-multi-fluorescence-imaging-system-for-teaching-and-research-in-biology-and-bioengineering
#11
Isaac Nuñez, Tamara Matute, Roberto Herrera, Juan Keymer, Timothy Marzullo, Timothy Rudge, Fernán Federici
The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates...
2017: PloS One
https://www.readbyqxmd.com/read/29099745/a-middleware-solution-for-wireless-iot-applications-in-sparse-smart-cities
#12
Paolo Bellavista, Carlo Giannelli, Stefano Lanzone, Giulio Riberto, Cesare Stefanelli, Mauro Tortonesi
The spread of off-the-shelf mobile devices equipped with multiple wireless interfaces together with sophisticated sensors is paving the way to novel wireless Internet of Things (IoT) environments, characterized by multi-hop infrastructure-less wireless networks where devices carried by users act as sensors/actuators as well as network nodes. In particular, the paper presents Real Ad-hoc Multi-hop Peer-to peer-Wireless IoT Application (RAMP-WIA), a novel solution that facilitates the development, deployment, and management of applications in sparse Smart City environments, characterized by users willing to collaborate by allowing new applications to be deployed on their smartphones to remotely monitor and control fixed/mobile devices...
November 3, 2017: Sensors
https://www.readbyqxmd.com/read/29090743/a-combination-of-raspberry-pi-and-softether-vpn-for-controlling-research-devices-via-the-internet
#13
Toshikazu Kuroda
Remote control over devices for experiments may increase the efficiency of operant research and expand the area where behavior can be studied. This article introduces a combination of Raspberry Pi® (Pi) and SoftEther VPN® that allows for such remote control via the Internet. The Pi is a small Linux computer with a great degree of flexibility for customization. Test results indicate that a Pi-based interface meets the requirement for conducting operant research. SoftEther VPN® allows for establishing an extensive private network on the Internet using a single private Wi-Fi router...
November 1, 2017: Journal of the Experimental Analysis of Behavior
https://www.readbyqxmd.com/read/29070625/individualized-tracking-of-self-directed-motor-learning-in-group-housed-mice-performing-a-skilled-lever-positioning-task-in-the-home-cage
#14
Gergely Silasi, Jamie Boyd, Federico Bolanos, Jeff LeDue, Stephen H Scott, Timothy H Murphy
Skilled forelimb function in mice is traditionally studied through behavioural paradigms that require extensive training by investigators, and are limited by the number of trials individual animals are able to perform within supervised session. We developed a skilled lever positioning task that mice can perform within their home cage. The task requires mice to use their forelimb to precisely hold a lever mounted on a rotary encoder within a rewarded position in order to dispense a water reward. A Raspberry Pi microcomputer is used to record lever position during trials, and to control task parameters, thus making this low-footprint apparatus ideal for use within animal housing facilities...
October 25, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/29060793/a-low-cost-video-oculography-system-for-vestibular-function-testing
#15
Jihwan Park, Youngsun Kong, Yunyoung Nam
In order to remain in focus during head movements, vestibular-ocular reflex causes eyes to move in the opposite direction to head movement. Disorders of vestibular system decrease vision, causing abnormal nystagmus and dizziness. To diagnose abnormal nystagmus, various studies have been reported including the use of rotating chair tests and videonystagmography. However, these tests are unsuitable for home use due to their high costs. Thus, a low-cost video-oculography system is necessary to obtain clinical features at home...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29049280/ethoscopes-an-open-platform-for-high-throughput-ethomics
#16
Quentin Geissmann, Luis Garcia Rodriguez, Esteban J Beckwith, Alice S French, Arian R Jamasb, Giorgio F Gilestro
Here, we present the use of ethoscopes, which are machines for high-throughput analysis of behavior in Drosophila and other animals. Ethoscopes provide a software and hardware solution that is reproducible and easily scalable. They perform, in real-time, tracking and profiling of behavior by using a supervised machine learning algorithm, are able to deliver behaviorally triggered stimuli to flies in a feedback-loop mode, and are highly customizable and open source. Ethoscopes can be built easily by using 3D printing technology and rely on Raspberry Pi microcomputers and Arduino boards to provide affordable and flexible hardware...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/28880156/a-high-resolution-mini-microscope-system-for-wireless-real-time-monitoring
#17
Zongjie Wang, Akash Boddeda, Benjamin Parker, Roya Samanipour, Sanjoy Ghosh, Frederic Menard, Keekyoung Kim
OBJECTIVE: Compact, cost-effective and high-performance microscope that enables the real-time imaging of cells and lab-on-a-chip devices is highly demanded for cell biology and biomedical engineering. This paper aims to present the design and application of an inexpensive wireless mini-microscope with resolution up to 2592 × 1944 pixels and speed up to 90 fps. METHODS: The mini-microscope system was built on a commercial embedded system (Raspberry Pi). We modified a camera module and adopted an inverse dual lens system to obtain the clear field of view and appropriate magnification for tens of micrometer objects...
September 4, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28860079/automated-touch-sensing-in-the-mouse-tapered-beam-test-using-raspberry-pi
#18
Dirk Jan Ardesch, Matilde Balbi, Timothy H Murphy
BACKGROUND: Rodent models of neurological disease such as stroke are often characterized by motor deficits. One of the tests that are used to assess these motor deficits is the tapered beam test, which provides a sensitive measure of bilateral motor function based on foot faults (slips) made by a rodent traversing a gradually narrowing beam. However, manual frame-by-frame scoring of video recordings is necessary to obtain test results, which is time-consuming and prone to human rater bias...
November 1, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28719603/the-%C3%A2-100-lab-a-3d-printable-open-source-platform-for-fluorescence-microscopy-optogenetics-and-accurate-temperature-control-during-behaviour-of-zebrafish-drosophila-and-caenorhabditis-elegans
#19
Andre Maia Chagas, Lucia L Prieto-Godino, Aristides B Arrenberg, Tom Baden
Small, genetically tractable species such as larval zebrafish, Drosophila, or Caenorhabditis elegans have become key model organisms in modern neuroscience. In addition to their low maintenance costs and easy sharing of strains across labs, one key appeal is the possibility to monitor single or groups of animals in a behavioural arena while controlling the activity of select neurons using optogenetic or thermogenetic tools. However, the purchase of a commercial solution for these types of experiments, including an appropriate camera system as well as a controlled behavioural arena, can be costly...
July 2017: PLoS Biology
https://www.readbyqxmd.com/read/28520533/a-fully-automated-high-throughput-zebrafish-behavioral-ototoxicity-assay
#20
Douglas W Todd, Rohit C Philip, Maki Niihori, Ryan A Ringle, Kelsey R Coyle, Sobia F Zehri, Leanne Zabala, Jordan A Mudery, Ross H Francis, Jeffrey J Rodriguez, Abraham Jacob
Zebrafish animal models lend themselves to behavioral assays that can facilitate rapid screening of ototoxic, otoprotective, and otoregenerative drugs. Structurally similar to human inner ear hair cells, the mechanosensory hair cells on their lateral line allow the zebrafish to sense water flow and orient head-to-current in a behavior called rheotaxis. This rheotaxis behavior deteriorates in a dose-dependent manner with increased exposure to the ototoxin cisplatin, thereby establishing itself as an excellent biomarker for anatomic damage to lateral line hair cells...
August 2017: Zebrafish
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