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tonicity and HSP70

Yufei Wang, Paul Lavender, Julie Watson, Matthew Arno, Thomas Lehner
The prevailing evidence suggests that immunological memory does not require antigenic re-stimulation but is maintained by low level tonic stimulation. We examined the hypothesis that stress agents contribute to tonic cellular activation and maintain immunological memory. Stimulation of monocyte-derived dendritic cells (DC) with stress agents elicits reactive oxygen species and HSP70. NFκB is activated, which up-regulates membrane-associated (ma) IL-15, caspase-1 and IL-1β. Co-culture of stress-treated DC with mononuclear cells activates IL-15 and IL-1β receptors on CD4(+) T cells, eliciting CD40L, proliferation, and up-regulation of CD45RO(+) memory T cells...
June 19, 2015: Journal of Biological Chemistry
Mehmet Kilic, Mehmet Gündüzalp, Erdal Taskin, Süleyman Aydin, Hepsen M Serin
BACKGROUND: In this study we aimed to evaluate the serum levels of Heat-shock protein (Hsp) 70 and acylated and desacylated ghrelin in patients suffering from a simple febrile convulsion. METHODS: This cross-sectional study included patients who were diagnosed with a simple febrile convulsion, afebrile tonic-clonic epileptic seizure and upper respiratory tract infection when admitted to our hospital. All patients were aged between six months and 60 months. Patients enrolled in this study were divided into five groups...
April 2016: Minerva Pediatrica
Juliane da Silva Rossato, Mauricio Krause, Augustus Joli Martins Fernandes, João Roberto Fernandes, Isis Lenhard Seibt, Anderson Rech, Paulo Ivo Homem de Bittencourt
Previous studies from our laboratory have demonstrated that a single bout of moderate exercise stimulates macrophage function, increasing phagocytic capacity, and production of hydrogen peroxide and nitric oxide (NO˙) through nuclear factor kappa B activation. In this work, we investigated the role of α- and β-adrenoreceptors on the function of monocyte/macrophages during rest and exercise. Adult male Wistar rats were i.p. administered (100 μL/100 g) with specific adrenergic antagonists before an acute moderate exercise bout: prazosin (α1-specific antagonist 2 mg/kg), propranolol (unspecific β1/β2 antagonist 10 mg/kg), double blockade (α1 and β1/β2), or phosphate-buffered saline (control)...
June 2014: Journal of Physiology and Biochemistry
Jing-Yang Wong, Mahmood Ameen Abdulla, Jegadeesh Raman, Chia-Wei Phan, Umah Rani Kuppusamy, Shahram Golbabapour, Vikineswary Sabaratnam
Hericium erinaceus is a famous tonic in oriental medicine. The gastroprotective effects of aqueous extract of H. erinaceus against ethanol-induced ulcers in Sprague Dawley rats were investigated. The possible involvements of lipid peroxidation, superoxide dismutase, and catalase were also investigated. Acute toxicity study was performed. The effects of aqueous extract of H. erinaceus on the ulcer areas, ulcer inhibition, gastric wall mucus, gross and histological gastric lesions, antioxidant levels, and malondialdehyde (MDA) contents were evaluated in ethanol-induced ulcer in vivo...
2013: Evidence-based Complementary and Alternative Medicine: ECAM
Alexzander Asea, Punit Kaur, Alexander Panossian, Karl Georg Wikman
We have previously demonstrated that ADAPT-232, a fixed combination of adaptogenic substances derived from Eleutherococcus senticosus root extract, Schisandra chinensis berry extract, Rhodiola rosea root extract stimulated the expression and release of neuropeptide Y (NPY) and molecular chaperone Hsp72 from isolated human neurolgia cells. Both of these mediators of stress response are known to play an important role in regulation of neuroendocrine system and immune response. We further demonstrated that ADAPT-232 induced release of Hsp70 is mediated by NPY, suggesting an existence of NPY-mediated pathway of activation of Hsp72 release into the blood circulation system...
November 15, 2013: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
A Al-Aqil, I Zulkifli, M Hair Bejo, A Q Sazili, M A Rajion, M N Somchit
An experiment was conducted to determine the effects of combining both pleasant and unpleasant contacts with human beings on physiology and behavior of broiler chickens. Birds were subjected to the following treatments: (i) received no physical or visual contact with humans (control); (ii) from d 1 to 28, chicks were individually stroked gently for 30 s once daily (PL); (iii) from d 1 to 28, chicks were picked up individually, suspended by both legs, exposed to recorded noise, and swung gently for 15 s once daily (UNPL); (iv) from d 1 to 14 and from d 15 to 28, chicks were subjected to PL and UNPL, respectively (PL-UNPL); and (v) from d 1 to 14 and from d 15 to 28, chicks were subjected to UNPL and PL, respectively (UNPL-PL)...
January 2013: Poultry Science
Konrad Rejdak, Jens Kuhle, Stephan Rüegg, Raija L P Lindberg, Axel Petzold, Dorota Sulejczak, Ewa Papuc, Robert Rejdak, Zbigniew Stelmasiak, Paweł Grieb
PURPOSE:   Status epilepticus (SE) has deleterious effects on brain tissue, but whether brief recurrent seizures may also damage neurons represents a matter of controversy. Therefore, it remains a central area of epilepsy research to identify individuals at risk where disease progression can be potentially prevented. Biomarkers may serve as tools for such identification. Thus the present study aimed at analyzing the levels of heat shock protein 70 (HSP-70, also designated as HSPA1A) and neurofilament heavy chain protein (NfH(SMI35) ) in cerebrospinal fluid (CSF) of patients with seizures of different severity...
May 2012: Epilepsia
Amin Afrazi, Chhinder P Sodhi, Misty Good, Hongpeng Jia, Richard Siggers, Ibrahim Yazji, Congrong Ma, Matthew D Neal, Thomas Prindle, Zachary S Grant, Maria F Branca, John Ozolek, Eugene B Chang, David J Hackam
Necrotizing enterocolitis (NEC) is the leading cause of gastrointestinal-related mortality in premature infants, and it develops under conditions of exaggerated TLR4 signaling in the newborn intestinal epithelium. Because NEC does not develop spontaneously, despite the presence of seemingly tonic stimulation of intestinal TLR4, we hypothesized that mechanisms must exist to constrain TLR4 signaling that become diminished during NEC pathogenesis and focused on the intracellular stress response protein and chaperone heat shock protein-70 (Hsp70)...
May 1, 2012: Journal of Immunology: Official Journal of the American Association of Immunologists
Shilpa S Gogate, Nobuyuki Fujita, Renata Skubutyte, Irving M Shapiro, Makarand V Risbud
The objective of our study was to examine the regulation of hypoxic expression of heat shock protein 70 (Hsp70) in nucleus pulposus cells and to determine if Hsp70 promoted hypoxia-inducible factor (HIF)-1α degradation. Rat nucleus pulposus cells were maintained in culture in either 21% or 1% oxygen. To determine the regulation of Hsp70 expression by tonicity enhancer binding protein (TonEBP) and HIF-1/2, loss-of-function and gain-of-function experiments and mutational analysis of the Hsp70 promoter were performed...
May 2012: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
Elena A Kharlamov, Eka Lepsveridze, Maia Meparishvili, Revaz O Solomonia, Bo Lu, Eric R Miller, Kevin M Kelly, Zakaria Mtchedlishvili
Traumatic brain injury (TBI) can result in the development of posttraumatic epilepsy (PTE). Recently, we reported differential alterations in tonic and phasic GABA(A) receptor (GABA(A)R) currents in hippocampal dentate granule cells 90 days after controlled cortical impact (CCI) (Mtchedlishvili et al., 2010). In the present study, we investigated long-term changes in the protein expression of GABA(A)R α1, α4, γ2, and δ subunits, NMDA (NR2B) and AMPA (GluR1) receptor subunits, and heat shock proteins (HSP70 and HSP90) in the hippocampus of Sprague-Dawley rats evaluated by Western blotting in controls, CCI-injured animals without PTE (CCI group), and CCI-injured animals with PTE (PTE group)...
June 2011: Epilepsy Research
Sang Do Lee, Soo Youn Choi, H Moo Kwon
During antidiuresis with elevated vasopressin, urea accumulates in the renal medulla to very high concentrations, imposing considerable cellular stress. How local cells cope with urea stress is relevant to the whole kidney because the renal medulla is the major site of residence for the renal stem cells. Previous studies showed that renal cells were incapable of preconditioning in moderate urea concentrations to enhance resistance to urea stress. Instead, preconditioning in moderately high salinity (moderate hypertonicity) has been shown to promote resistance to urea stress due to the induction of the molecular chaperone heat shock protein 70 (Hsp70), which is mediated by the transcription factor tonicity-responsive enhancer binding protein (TonEBP)...
March 2011: American Journal of Physiology. Cell Physiology
L E Nitsinskaia, I V Ekimova, I V Guzhova, B A Feĭzulaev, Iu F Pastukhov
Effects of the inhibitor of the expression of Heat shock proteins 70 kDa (Hsp70), quercetin on seizures and movement disorders induced by N-methyl D-aspartate (NMDA) or pentylenetetrazole in adult rats Wistar were investigated using behavioral methods. It was found that intraperitoneal injection of quercetin 4 hours before intraventricular microinjection of NMDA resulted in increased duration of tonic component of seizures, seizure and ataxia symptoms severity. Blockade of the expression of Hsp70 by quercetin increased the duration of clonic and tonic seizures and did not affect severity of seizures and ataxia symptoms, induced by intraperitoneal injection of pentylenetetrazole...
March 2010: Rossiĭskii Fiziologicheskiĭ Zhurnal Imeni I.M. Sechenova
Hardeep Kataria, Navjot Shah, Sunil C Kaul, Renu Wadhwa, Gurcharan Kaur
Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays...
2011: Evidence-based Complementary and Alternative Medicine: ECAM
I Zulkifli, A Al-Aqil, A R Omar, A Q Sazili, M A Rajion
Two hundred thirty-five 1-d-old broiler chickens showing short or long tonic immobility responses were classified as low fear (LF) or high fear (HF) responders, respectively. On d 41, they were subjected to either crating or heat challenge (34 +/- 1 degrees C) for 3 h and its effect on plasma corticosterone concentration, heterophil/lymphocyte ratios, and heat shock protein (HSP) 70 expression in brain tissue were determined. Crating and heat exposure elevated heterophil/lymphocyte ratios in both LF and HF birds...
March 2009: Poultry Science
Yun Sook Lim, Jae Seon Lee, Tai Qin Huang, Jeong Sun Seo
Heat shock protein 70 (HSP70), which evidences important functions as a molecular chaperone and anti-apoptotic molecule, is substantially induced in cells exposed to a variety of stresses, including hypertonic stress, heavy metals, heat shock, and oxidative stress, and prevents cellular damage under these conditions. However, the molecular mechanism underlying the induction of HSP70 in response to hypertonicity has been characterized to a far lesser extent. In this study, we have investigated the cellular signaling pathway of HSP70 induction under hypertonic conditions...
December 31, 2008: Experimental & Molecular Medicine
Paola Navarro, Mario Chiong, Karen Volkwein, Francisco Moraga, María Paz Ocaranza, Jorge E Jalil, Sun Woo Lim, Jeong-Ah Kim, H Moo Kwon, Sergio Lavandero
Changes in cardiac osmolarity occur in myocardial infarction. Osmoregulatory mechanisms may, therefore, play a crucial role in cardiomyocyte survival. Tonicity-responsive enhancer binding protein (TonEBP) is a key transcription factor participating in the adaptation of cells to increases in tonicity. However, it is unknown whether cardiac TonEBP is activated by tonicity. Hypertonicity activated transcriptional activity of TonEBP, increased the amounts of both TonEBP mRNA and protein, and induced both the mRNA and protein of TonEBP target genes (aldose reductase and heat shock protein-70)...
July 25, 2008: Biochemical and Biophysical Research Communications
Carlos E Irarrazabal, Chester K Williams, Megan A Ely, Michael J Birrer, Arlyn Garcia-Perez, Maurice B Burg, Joan D Ferraris
Tonicity-responsive enhancer/osmotic response element-binding protein (TonEBP/OREBP) is a Rel protein that activates transcription of osmoprotective genes at high extracellular NaCl. Other Rel proteins NFAT1-4 and NF-kappaB complex with activator protein-1 (AP-1) to transactivate target genes through interaction at composite NFAT/NF-kappaB.AP-1 sites. TonEBP/OREBP target genes commonly have one or more conserved AP-1 binding sites near TonEBP/OREBP cognate elements (OREs). Also, TonEBP/OREBP and the AP-1 proteins c-Fos and c-Jun are all activated by high NaCl...
February 1, 2008: Journal of Biological Chemistry
Wolfgang Neuhofer, Daniela Steinert, Maria-Luisa Fraek, Franz-X Beck
The cells of the renal medulla produce large amounts of prostaglandin E2 (PGE2) via cyclooxygenases (COX)-1 and -2. PGE2 is well known to play a critical role in salt and water balance and maintenance of medullary blood flow. Since renal medullary PGE2 production increases in antidiuresis, and since COX inhibition is associated with damage to the renal medulla during water deprivation, PGE2 may promote the adaptation of renal papillary cells to high interstitial solute concentrations. To address this question, MDCK cells were exposed to a gradual tonicity increase in the presence or absence of 20 microM PGE2 prior to analysis of (i) cell survival, (ii) expression of osmoprotective genes (AR, BGT1, SMIT, HSP70 and COX-2), (iii) subcellular TonEBP/NFAT5 abundance, (iv) TonEBP/NFAT5 transcriptional activity and (v) aldose reductase promoter activity...
August 15, 2007: Journal of Physiology
Un Sil Jeon, Ki-Hwan Han, Soo-Hyun Park, Sang Do Lee, Mee Rie Sheen, Ju-Young Jung, Wan Young Kim, Jeff M Sands, Jin Kim, H Moo Kwon
Hypokalemia causes a significant decrease in the tonicity of the renal medullary interstitium in association with reduced expression of sodium transporters in the distal tubule. We asked whether hypokalemia caused downregulation of the tonicity-responsive enhancer binding protein (TonEBP) transcriptional activator in the renal medulla due to the reduced tonicity. We found that the abundance of TonEBP decreased significantly in the outer and inner medullas of hypokalemic rats. Underlying mechanisms appeared different in the two regions because the abundance of TonEBP mRNA was lower in the outer medulla but unchanged in the inner medulla...
July 2007: American Journal of Physiology. Renal Physiology
Susanne Ammon-Treiber, Gisela Grecksch, Charalampos Angelidis, Patra Vezyraki, Volker Höllt, Axel Becker
Kindling induced by the convulsant pentylenetetrazol (PTZ) is an accepted model of primary generalized epilepsy. Because seizures represent a strong distressing stimulus, stress-induced proteins such as heat shock proteins might counteract the pathology of increased neuronal excitation. Therefore, the aim of the present study was to determine whether PTZ kindling outcome parameters are influenced by heat shock protein 70 (Hsp70) overexpression in Hsp70 transgenic mice as compared to the respective wild-type mice...
April 2007: Naunyn-Schmiedeberg's Archives of Pharmacology
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