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https://www.readbyqxmd.com/read/27809315/rapid-identification-of-causal-mutations-in-tomato-ems-populations-via-mapping-by-sequencing
#1
Virginie Garcia, Cécile Bres, Daniel Just, Lucie Fernandez, Fabienne Wong Jun Tai, Jean-Philippe Mauxion, Marie-Christine Le Paslier, Aurélie Bérard, Dominique Brunel, Koh Aoki, Saleh Alseekh, Alisdair R Fernie, Paul D Fraser, Christophe Rothan
The tomato is the model species of choice for fleshy fruit development and for the Solanaceae family. Ethyl methanesulfonate (EMS) mutants of tomato have already proven their utility for analysis of gene function in plants, leading to improved breeding stocks and superior tomato varieties. However, until recently, the identification of causal mutations that underlie particular phenotypes has been a very lengthy task that many laboratories could not afford because of spatial and technical limitations. Here, we describe a simple protocol for identifying causal mutations in tomato using a mapping-by-sequencing strategy...
December 2016: Nature Protocols
https://www.readbyqxmd.com/read/27706237/inducible-expression-of-the-de-novo-designed-antimicrobial-peptide-sp1-1-in-tomato-confers-resistance-to-xanthomonas-campestris-pv-vesicatoria
#2
Areli Herrera Diaz, Izabella Kovacs, Christian Lindermayr
Antimicrobial peptides (AMPs) are small peptides with less than 50 amino acids and are part of the innate immune response in almost all organisms, including bacteria, vertebrates, invertebrates and plants. AMPs are active against a broad-spectrum of pathogens. The inducible expression of AMPs in plants is a promising approach to combat plant pathogens with minimal negative side effects, such as phytotoxicity or infertility. In this study, inducible expression of the de-novo designed AMP SP1-1 in Micro Tom tomato protected tomato fruits against bacterial spot disease caused by Xanthomonas campestris pv...
2016: PloS One
https://www.readbyqxmd.com/read/27679646/selection-of-reference-genes-for-quantitative-real-time-rt-pcr-studies-in-tomato-fruit-of-the-genotype-mt-rg1
#3
Karla L González-Aguilera, Carolina F Saad, Ricardo A Chávez Montes, Marcio Alves-Ferreira, Stefan de Folter
Quantitative real-time RT-PCR (qRT-PCR) has become one of the most widely used methods for accurate quantification of gene expression. Since there are no universal reference genes for normalization, the optimal strategy to normalize raw qRT-PCR data is to perform an initial comparison of a set of independent reference genes to assess the most stable ones in each biological model. Normalization of a qRT-PCR experiment helps to ensure that the results are both statistically significant and biologically meaningful...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27646210/characterization-and-evaluation-of-bacillus-amyloliquefaciens-strain-wf02-regarding-its-biocontrol-activities-and-genetic-responses-against-bacterial-wilt-in-two-different-resistant-tomato-cultivars
#4
Chu-Ning Huang, Chan-Pin Lin, Feng-Chia Hsieh, Sook-Kuan Lee, Kuan-Chen Cheng, Chi-Te Liu
Bacillus amyloliquefaciens strain WF02, isolated from soil collected at Wufeng Mountain, Taiwan, has siderophore-producing ability and in vitro antagonistic activity against bacterial wilt pathogen. To determine the impact of plant genotype on biocontrol effectiveness, we treated soil with this strain before infecting susceptible (L390) and moderately resistant (Micro-Tom) tomato cultivars with Ralstonia solanacearum strain Pss4. We also compared the efficacy of this strain with that of commercial Bacillus subtilis strain Y1336...
November 2016: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27545651/small-heat-shock-proteins-and-the-postharvest-chilling-tolerance-of-tomato-fruit
#5
Martín D Ré, Carla Gonzalez, Mariela R Escobar, María Laura Sossi, Estela M Valle, Silvana B Boggio
Plants have the largest number of small heat shock proteins (sHsps) (15-42 kDa) among eukaryotes, but little is known about their function in vivo. They accumulate in response to different stresses, and specific sHsps are also expressed during developmental processes such as seed development, germination, and ripening. The presence of organelle-specific sHsps appears to be unique to plants. The sHsps expression is regulated by heat stress transcription factors (Hsfs). In this work, it was explored the role of sHsps in the chilling injury of tomato fruit...
August 22, 2016: Physiologia Plantarum
https://www.readbyqxmd.com/read/27454588/expression-of-the-theobroma-cacao-bax-inhibitor-1-gene-in-tomato-reduces-infection-by-the-hemibiotrophic-pathogen-moniliophthora-perniciosa
#6
Danielle Camargo Scotton, Mariana da Silva Azevedo, Ivan Sestari, Jamille Santos da Silva, Lucas Anjos Souza, Lázaro Eustáquio Pereira Peres, Gildemberg Amorim Leal, Antonio Figueira
Programmed cell death (PCD) plays a key role in plant responses to pathogens, determining the success of infection depending on the pathogen lifestyle and on which participant of the interaction triggers cell death. The hemibiotrophic basidiomycete Moniliophthora perniciosa is the causal agent of the witches' broom disease of Theobroma cacao L (cacao), a serious constraint for production in South America and Caribbean. It has been hypothesized that M. perniciosa pathogenesis involves PCD, initially as a plant defense mechanism, which is diverted by the fungus to induce necrosis during the dikaryotic phase of the mycelia...
July 25, 2016: Molecular Plant Pathology
https://www.readbyqxmd.com/read/27409224/effects-of-flooding-stress-in-micro-tom-tomato-plants-transformed-with-different-levels-of-mitochondrial-shsp23-6
#7
C M Hüther, E G Martinazzo, C V Rombaldi, M A Bacarin
Soil flooding is an environmental stressor for crops that can affect physiological performance and reduce crop yields. Abiotic stressors cause changes in protein synthesis, modifying the levels of a series of proteins, especially the heat shock proteins (HSP), and these proteins can help protect the plants against abiotic stress. The objective of this study was to verify if tomato plants cv. Micro-Tom from different genotypes with varying expression levels of MT-sHSP23.6 (mitochondrial small heat shock proteins) have different responses physiological to flooding...
July 11, 2016: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/27396123/-effects-of-acaulospora-spinosa-on-plant-growth-and-lycopene-related-genes-psyl-and-psy2-expression-of-tomato
#8
Ke Li, Jian-jun Xu, Lu-yao Wang, Chu-nan Wang, Chen Yu, Jian-hua Guo
In the present study, an arbuscular mycorrhizal fungus (AMF) strain Acaulospora spinosa XJ27, previously isolated from tomato root rhizosphere in Xinjiang' s suburbs, was used to inoculate root systems of tomato cultivar "Micro-Tom". The biomass, contents of chlorophyll in leaves and lycopene in fruits of plants between the treatments with and without XJ27 were analyzed, and the expression level of lycopene biosynthesis relevant genes (psyl and.psy2) investigated by semi quantitative-PCR and real-time PCR. The results indicated that the application of XJ27 to the rhizosphere of tomato increased the biomass and yield of the treated plants, the expression of psyl and psy2 was improved, and the amount of lycopene accumulation in tomato...
February 2016: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/27263082/abscisic-acid-deficient-sit-tomato-mutant-responses-to-cadmium-induced-stress
#9
Georgia B Pompeu, Milca B Vilhena, Priscila L Gratão, Rogério F Carvalho, Mônica L Rossi, Adriana P Martinelli, Ricardo A Azevedo
There is a very effective cross-talk between signals triggered by reactive oxygen species and hormonal responses in plants, activating proteins/enzymes likely to be involved in stress tolerance. Abscisic acid (ABA) is known as a stress hormone that takes part in the integration of signals. This work aimed to characterize the biochemical response and ultrastructural changes induced by cadmium (Cd) in the Micro-Tom (MT) sitiens ABA-deficient mutant (sit) and its wild-type (MT) counterpart. MT and sit plants were grown over a 96-h period in the presence of Cd (0, 10, and 100 μM CdCl2)...
June 4, 2016: Protoplasma
https://www.readbyqxmd.com/read/27208295/the-glycerol-3-phosphate-acyltransferase-gpat6-from-tomato-plays-a-central-role-in-fruit-cutin-biosynthesis
#10
Johann Petit, Cécile Bres, Jean-Philippe Mauxion, Fabienne Wong Jun Tai, Laetitia B B Martin, Eric A Fich, Jérôme Joubès, Jocelyn K C Rose, Frédéric Domergue, Christophe Rothan
The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micro-Tom and isolated several glossy fruit mutants in which the abundance of cutin, the polyester component of the cuticle, was strongly reduced. We employed a newly developed mapping-by-sequencing strategy to identify the causal mutation underlying the cutin deficiency in a mutant thereafter named gpat6-a (for glycerol-3-phosphate acyltransferase6)...
June 2016: Plant Physiology
https://www.readbyqxmd.com/read/27052409/proteome-modification-in-tomato-plants-upon-long-term-aluminum-treatment
#11
Suping Zhou, Ikenna Okekeogbu, Sasikiran Sangireddy, Zhujia Ye, Hui Li, Sarabjit Bhatti, Dafeng Hui, Daniel W McDonald, Yong Yang, Shree Giri, Kevin J Howe, Tara Fish, Theodore W Thannhauser
This study aimed to identify the aluminum (Al)-induced proteomes in tomato (Solanum lycopersicum, "Micro-Tom") after long-term exposure to the stress factor. Plants were treated in Magnavaca's solution (pH 4.5) supplemented with 7.5 μM Al(3+) ion activity over a 4 month period beginning at the emergence of flower buds and ending when the lower mature leaves started to turn yellow. Proteomes were identified using a 8-plex isobaric tags for relative and absolute quantification (iTRAQ) labeling strategy followed by a two-dimensional (high- and low-pH) chromatographic separation and final generation of tandem mass spectrometry (MS/MS) spectra of tryptic peptides on an LTQ-Orbitrap Elite mass spectrometer...
May 6, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/26891589/root-growth-restraint-can-be-an-acclimatory-response-to-low-ph-and-is-associated-with-reduced-cell-mortality-a-possible-role-of-class-iii-peroxidases-and-nadph-oxidases
#12
J P Graças, R Ruiz-Romero, L D Figueiredo, L Mattiello, L E P Peres, V A Vitorello
Low pH (<5.0) can significantly decrease root growth but whether this is a direct effect of H(+) or an active plant response is examined here. Tomato (Solanum lycopersicum cv Micro-Tom) roots were exposed directly or gradually to low pH through step-wise changes in pH over periods ranging from 4 to 24 h. Roots exposed gradually to pH 4.5 grew even less than those exposed directly, indicating a plant-coordinated response. Direct exposure to pH 4.0 suppressed root growth and caused high cell mortality, in contrast to roots exposed gradually, in which growth remained inhibited but cell viability was maintained...
July 2016: Plant Biology
https://www.readbyqxmd.com/read/26869236/effect-on-tomato-plant-and-fruit-of-the-application-of-biopolymer-oregano-essential-oil-coatings
#13
Ángela Perdones, Núria Tur, Amparo Chiralt, Maria Vargas
BACKGROUND: Oregano essential oil (EO) was incorporated into film-forming dispersions (FFDs) based on biopolymers (chitosan and/or methylcellulose) at two different concentrations. The effect of the application of the FFDs was evaluated on tomato plants (cultivar Micro-Tom) at three different stages of development, and on pre-harvest and postharvest applications on tomato fruit. RESULTS: The application of the FFDs at '3 Leaves' stage caused phytotoxic problems, which were lethal when the EO was applied without biopolymers...
October 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/26371478/plastid-proteomic-analysis-in-tomato-fruit-development
#14
Miho Suzuki, Sachiko Takahashi, Takanori Kondo, Hideo Dohra, Yumihiko Ito, Yoshikazu Kiriiwa, Marina Hayashi, Shiori Kamiya, Masaya Kato, Masayuki Fujiwara, Yoichiro Fukao, Megumi Kobayashi, Noriko Nagata, Reiko Motohashi
To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and plastid changes over four distinct developmental stages of 'Micro-Tom' fruit. Additionally, to discover more about the relationship between fruit color and plastid differentiation, we also analyzed and compared 'Micro-Tom' results with those from two other varieties, 'Black' and 'White Beauty'. We confirmed that proteins related to photosynthesis remain through the orange maturity stage of 'Micro-Tom', and also learned that thylakoids no longer exist at this stage...
2015: PloS One
https://www.readbyqxmd.com/read/26344339/isolation-of-ahl-degrading-bacteria-from-micro-algal-cultures-and-their-impact-on-algal-growth-and-on-virulence-of-vibrio-campbellii-to-prawn-larvae
#15
Gde Sasmita Julyantoro Pande, Fatin Mohd Ikhsan Natrah, Ace Vincent Bravo Flandez, Uday Kumar, Yufeng Niu, Peter Bossier, Tom Defoirdt
Inactivation of quorum sensing (QS) signal molecules, such as acylhomoserine lactones (AHLs) of pathogenic bacteria, has been proposed as a novel method to combat bacterial diseases in aquaculture. Despite the importance of micro-algae for aquaculture, AHL degradation by bacteria associated with micro-algal cultures has thus far not been investigated. In this study, we isolated Pseudomonas sp. NFMI-T and Bacillus sp. NFMI-C from open cultures of the micro-algae Tetraselmis suecica and Chaetoceros muelleri, respectively...
December 2015: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26252379/cancer-associated-fibroblasts-in-stage-i-iiia-nsclc-prognostic-impact-and-their-correlations-with-tumor-molecular-markers
#16
Thomas K Kilvaer, Mehrdad Rakaee Khanehkenari, Turid Hellevik, Samer Al-Saad, Erna-Elise Paulsen, Roy M Bremnes, Lill-Tove Busund, Tom Donnem, Inigo Z Martinez
BACKGROUND: Cancer Associated Fibroblasts (CAFs) are thought to regulate tumor growth and metastasis. Fibroblast Activating Protein 1 (FAP-1) is a marker for fibroblast activation and by many recognized as the main marker of CAFs. Alpha Smooth Muscle Actin (α-SMA) is a general myofibroblast marker, and can be used to identify CAFs. This study investigates the prognostic impact of FAP-1 and α-SMA in non-small cell lung cancer (NSCLC) patients and correlates their expression to 105 proteins investigated in the same cohort...
2015: PloS One
https://www.readbyqxmd.com/read/26197088/fucoidan-prevents-the-progression-of-osteoarthritis-in-rats
#17
Don-Gil Lee, Sang-Yong Park, Won-Seok Chung, Jae-Hee Park, Eunson Hwang, Gafurjon Tom Mavlonov, In-Ho Kim, Ki-Young Kim, Tae-Hoo Yi
This study investigated the effects of fucoidan (extract from Hizikia fusiforme) on symptoms and inflammatory cytokine activation in rats with monosodium iodoacetate (MIA)-induced osteoarthritis (OA). Forty male SD rats were divided into five groups, including normal, negative control (MIA), positive control (Lyprinol), and two experimental groups treated with 50 or 100 mg/kg fucoidan. Weight-bearing assessments were done after MIA injection into the right knee to induce OA. After 14 days of treatment, microcomputed tomographic (micro-CT) images were made of rat knee joints, and then animals were sacrificed for joint histology and inflammatory cytokine level assessments...
September 2015: Journal of Medicinal Food
https://www.readbyqxmd.com/read/26150302/the-effect-of-ego-motion-on-environmental-monitoring
#18
Uri Lerner, Tamar Yacobi, Ilan Levy, Sharon A Moltchanov, Tom Cole-Hunter, Barak Fishbain
Air pollution has a proven impact on public health. Currently, pollutant levels are obtained by high-priced, sizeable, stationary Air Quality Monitoring (AQM) stations. Recent developments in sensory and communication technologies have made relatively low-cost, micro-sensing units (MSUs) feasible. Their lower power consumption and small size enable mobile sensing, deploying single or multiple units simultaneously. Recent studies have reported on measurements acquired by mobile MSUs, mounted on cars, bicycles and pedestrians...
November 15, 2015: Science of the Total Environment
https://www.readbyqxmd.com/read/26077192/cadmium-stress-antioxidant-responses-and-root-to-shoot-communication-in-grafted-tomato-plants
#19
Priscila Lupino Gratão, Carolina Cristina Monteiro, Tiago Tezotto, Rogério Falleiros Carvalho, Letícia Rodrigues Alves, Leila Priscila Peters, Ricardo Antunes Azevedo
Many aspects related to ROS modulation of signaling networks and biological processes that control stress responses still remain unanswered. For this purpose, the grafting technique may be a powerful tool to investigate stress signaling and specific responses between plant organs during stress. In order to gain new insights on the modulation of antioxidant stress responses mechanisms, gas-exchange measurements, lipid peroxidation, H2O2 content, proline, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX) and guaiacol peroxidase (GPOX) were analyzed in Micro-Tom grafted plants submitted to cadmium (Cd)...
October 2015: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/26071544/mechanical-support-of-the-pressure-overloaded-right-ventricle-an-acute-feasibility-study-comparing-low-and-high-flow-support
#20
Tom Verbelen, Jelle Verhoeven, Motohiko Goda, Daniel Burkhoff, Marion Delcroix, Filip Rega, Bart Meyns
The objectives of this study were to assess the feasibility of low flow right ventricular support and to describe the hemodynamic effects of low versus high flow support in an animal model of acute right ventricular pressure overload. A Synergy Pocket Micro-pump (HeartWare International, Framingham, MA) was implanted in seven sheep. Blood was withdrawn from the right atrium to the pulmonary artery. Hemodynamics and pressure-volume loops were recorded in baseline conditions, after banding the pulmonary artery, and after ligating the right coronary artery in these banded sheep...
August 15, 2015: American Journal of Physiology. Heart and Circulatory Physiology
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