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https://www.readbyqxmd.com/read/28934012/liquid-chromatography-tandem-mass-spectrometry-method-for-simultaneous-quantification-of-azoxystrobin-and-its-metabolites-azoxystrobin-free-acid-and-2-hydroxybenzonitrile-in-greenhouse-grown-lettuce
#1
Maheswor Gautam, Inge S Fomsgaard
Lettuce is an important part of the diet in Europe. The permitted levels of pesticides in lettuce are strictly regulated and there is growing urge among food safety authorities to analyse pesticide metabolites as well. Azoxystrobin is one of pesticides that is frequently detected in lettuce. Although there are several analytical methods for the determination of azoxystrobin in lettuce, a sensitive method for the determination of its metabolites in lettuce is lacking. This study aimed at developing an extraction and LC-MS/MS method for the simultaneous determination of azoxystrobin, and its metabolites azoxystrobin free acid and 2-hydroxybenzonitrile in lettuce...
September 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28933085/-effects-of-different-concentrations-of-nitrogen-and-phosphorus-on-growth-and-active-components-of-salvia-miltiorrhiza
#2
Gui-Hui Xia, Qiu-Ling Wang, Wen-Quan Wang, Jun-Ling Hou, Qing-Yan Song, Lin Luo, Dou-Dou Zhang, Xiang Yang
With annual Salvia miltiorrhiza seedlings as experimental material, using "3414" optimal regression design recommended by the Ministry of Agriculture and regularly watered with nutrient solution, through the dynamic sampling of S. miltiorrhiza in different growing stages, and the growth index, dry weight of plant root and content of active components were measured. The potted experiments were applied to study the effects of different nitrogen and phosphorus ratios on the growth, dry matter accumulation and accumulation of active components of S...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28933037/effects-of-strain-sex-and-season-on-body-weight-development-of-cane-rat-thryonomys-swinderianus-in-the-humid-tropics
#3
Oluwatosin M A Jesuyon
The effects of strain (G), sex (H), and season (S) on live weight development of cane rats (Thryonomys swinderianus) reared in captive system were investigated during the dry (35.95 °C) and wet seasons (25.81 °C) from February to August of a tropical environment. The field experiment took place at the University of Ibadan. Seventy-nine cane rats spreading among three genotypes (Bamidele, FRIN, and Lawole) of different age and body weight groups were randomly distributed into cages using randomized complete block design in factorial...
September 20, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28933021/sources-composition-and-spectroscopiccharacteristics-of-dissolved-organic-matter-extractedfrom-sediments-in-an-anthropogenic-impacted-riverin-southeastern-china
#4
Tongbin Zhu, Pengfei Duan, Jianguo He, Miaomiao Zhao, Ming Li
The fluorescence and ultraviolet spectroscopic characteristics of dissolved organic matter extracted from sediments in an anthropogenic-impacted river (Hao River) in Southeastern China were analyzed in the current study. Excitation-emission matrix (EEM) fluorescence combined with parallel factor analysis (PARAFAC) was conducted to analyze the sources and composition of dissolved organic matter (DOM). The average dissolved organic carbon (DOC) concentrations of all samples at depths of 0-10, 10-20, and 20-30 cm were 317, 319, and 319 mg kg(-1) dry weight, respectively...
September 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28932675/osmotic-engine-translating-osmotic-pressure-into-macroscopic-mechanical-force-via-poly-acrylic-acid-based-hydrogels
#5
Lukas Arens, Felix Weißenfeld, Christopher O Klein, Karin Schlag, Manfred Wilhelm
Poly(acrylic acid)-based hydrogels can swell up to 100-1000 times their own weight in desalinated water due to osmotic forces. As the swelling is about a factor of 2-12 lower in seawater-like saline solutions (4.3 wt% NaCl) than in deionized water, cyclic swelling, and shrinking can potentially be used to move a piston in an osmotic motor. Consequently, chemical energy is translated into mechanical energy. This conversion is driven by differences in chemical potential and by changes in entropy. This is special, as most thermodynamic engines rely instead on the conversion of heat into mechanical energy...
September 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28928757/development-of-a-critical-nitrogen-dilution-curve-based-on-leaf-area-duration-in-wheat
#6
Xiaolong Wang, Tianyang Ye, Syed Tahir Ata-Ul-Karim, Yan Zhu, Leilei Liu, Weixing Cao, Liang Tang
Precise quantification of plant nitrogen (N) nutrition status is essential for crop N management. The concept of critical N concentration (Nc) has been widely used for assessment of plant N status. This study aimed to develop a new winter wheat Nc dilution curve based on leaf area duration (LAD). Four field experiments were performed on different cultivars with different N fertilization modes in the Yangtze River basin and Yellow River basin in China. Results showed that the increase in LAD with increasing cumulative thermal time took the shape of an "S" type curve; whereas shoot N concentration decreased with increasing LAD, according to a power function...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28928536/reduction-of-salt-content-of-fish-sauce-by-ethanol-treatment
#7
Yu Liu, Ying Xu, Xiaoxia He, Dongfeng Wang, Shiwei Hu, Shijie Li, Wei Jiang
Fish sauce is a traditional condiment in Southeast Asia, normally containing high concentration of salt. The solubility of salt is lower in ethanol than in water. In the present study, fish sauce was desalted by ethanol treatment (including the processes of ethanol addition, mixing, standing and rotary evaporation). The salt concentration of fish sauce decreased significantly from 29.72 to 19.72 g/100 mL when the treated ethanol concentration was 21% (v/v). The addition of more than 12% (v/v) of ethanol significantly reduced dry weight, total soluble nitrogen content and amino acids nitrogen content...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28928514/component-analysis-of-nutritionally-rich-chloroplasts-recovery-from-conventional-and-unconventional-green-plant-species
#8
Mohamed A Gedi, Rhianna Briars, Felius Yuseli, Noorazwani Zainol, Randa Darwish, Andrew M Salter, David A Gray
A study of the literature indicates that chloroplasts synthesise a range of molecules, many of which have nutritional value for humans, but the nutritional credentials of chloroplasts recovered from plant cells are not established. Chloroplast-rich-fractions (CRFs) were prepared from green plant species and the macro- and micro-nutrient composition compared with the whole leaf materials (WLMs). The results indicated that, on a dry weight basis, CRF material from a range of green biomass was enriched in lipids and proteins, and in a range of micronutrients compared with the WLM...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28927626/digestibility-of-starches-isolated-from-stem-and-root-tubers-of-arracacha-cassava-cush-cush-yam-potato-and-taro
#9
Mighay Lovera, Elevina Pérez, Alexander Laurentin
Digestibility of arracacha, cassava, cush-cush yam, potato and taro starches was evaluated. In vitro (potentially-available starch and total resistant starch) and in vivo digestibility in the rice weevil (Sitophilus oryzae) bioassay (survival, weight variation, α-amylase like activity [ALA], and uric acid excretion [UAE] as biomarkers) were estimated. In in vitro assays, all starches presented high resistant starch content (14-56%, dry basis), except for cassava starch. In in vivo assays, cush-cush yam and potato starches promoted higher ALA (>3 times) and UAE (>4 times) compare to a reference diet (cornstarch), in agreement to their low digestibility...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28923527/symptom-intensity-of-hospice-patients-a-longitudinal-analysis-of-concordance-between-patients-and-nurses-outcomes
#10
Everlien de Graaf, Daniëlle Zweers, Alexander de Graeff, Rebecca K Stellato, Saskia Teunissen
Nearing death, hospice patients are increasingly unable or unwilling to self-report their symptom intensity and rely on nurses' assessments. We hypothesized that concordance between patients' and nurses' assessments of symptom intensity improves over time. METHOD: A prospective longitudinal study was conducted from January 2012 to June 2015 using dyads of patients' and nurses' reported outcome measures, collected in daily hospice practice in the first three weeks after admission. Main outcomes were symptom intensity and well-being, measured using the Utrecht Symptom Diary (USD) and USD-Professional (USD-P)...
September 15, 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28922845/pot1-mediated-%C3%AE-integration-strategy-for-high-copy-stable-expression-of-heterologous-proteins-in-saccharomyces-cerevisiae
#11
Xiaofei Song, Quanli Liu, Jiwei Mao, Yuzhen Wu, Yuanzi Li, Kai Gao, Xiuming Zhang, Yanling Bai, Haijin Xu, Mingqiang Qiao
In biotechnological industry, increased expression cassette stability and copy number serve as important means of maintaining consistently high production levels of heterologous proteins in Saccharomyces cerevisiae. In this study, we combined δ sequences for site-specific integration with TPI1 gene from Schizosaccharomyces pombe (POT1) as a selection marker to realize high-copy integration and stable expression of heterologous proteins in S. cerevisiae. With the newly developed POT1 platform, a 32-copy integration of enhanced green fluorescent protein (EGFP) expression cassette was obtained in a single round and was stably maintained after 100 generations of growth in a rich complex medium...
September 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28922725/ameliorative-impact-of-morus-alba-leaves-aqueous-extract-against-embryonic-ophthalmic-tissue-malformation-in-streptozotocin-induced-diabetic-rats
#12
Adel M A Assiri, Mohamed E El-Beeh, Ali H Amin, Mohamed Fawzy Ramadan
BACKGROUND: Diabetes mellitus (DM) is becoming a serious threat to human health. Morus alba var. multicaulis (Perr.) Loudon (Moraceae) showed a bright future in DM therapy. OBJECTIVE: The study evaluates the antioxidant activity of Morus alba leaves aqueous extract (MLAE) and antidiabetic properties of MLAE in streptozotocin-induced diabetic rats focusing on the ameliorative effects against embryogenesis defects. MATERIALS AND METHODS: MLAE was assayed for bioactive compounds, and antiradical potential...
September 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28922610/symbiosis-of-selected-rhizobium-leguminosarum-bv-viciae-strains-with-diverse-pea-genotypes-effects-on-biological-nitrogen-fixation
#13
Chao Yang, Rosalind Bueckert, Jeff Schoenau, Axel Diederichsen, Hossein Zakeri, Thomas Warkentin
Biological nitrogen fixation (BNF) can be improved by optimizing the interaction between the rhizobial inoculant and pea (Pisum sativum L.), leading to increased productivity and reduced nitrogen (N) fertilizer use. Eight Rhizobium leguminosarum bv. viciae strains were used to inoculate super-nodulating pea mutant Rondo-nod3(fix+), hyper-nodulating pea mutant Frisson P88 Sym29, CDC Meadow commercial control, and a non-nodulating mutant Frisson P56 (nod-) to evaluate BNF in a greenhouse assay. Significant differences in strain×cultivar interactions were detected for shoot and root dry weight which ranged from 1...
September 18, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28921432/demonstration-scale-high-cell-density-fermentation-of-pichia-pastoris
#14
Wan-Cang Liu, Ping Zhu
Pichia pastoris has been one of the most successful heterologous overexpression systems in generating proteins for large-scale production through high-cell-density fermentation. However, optimizing conditions of the large-scale high-cell-density fermentation for biochemistry and industrialization is usually a laborious and time-consuming process. Furthermore, it is often difficult to produce authentic proteins in large quantities, which is a major obstacle for functional and structural features analysis and industrial application...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28921399/artesunate-inhibits-renal-ischemia-reperfusion-stimulated-lung-inflammation-in-rats-by-activating-ho-1-pathway
#15
Zhaohui Liu, Junjie Zhang, Shitong Li, Jihong Jiang
Artesunate (AS), a semi-synthetic derivative of Artemisia, has been shown to exert a wide range of pharmacological effects, such as anti-inflammatory and antioxidant functions. However, the protective functions of AS on renal ischemia reperfusion injury (RIR)-stimulated lung inflammation remain unclear. In this research, acute lung injury (ALI) was stimulated by renal ischemia reperfusion injury (RIR). AS (15 mg/kg) was intraperitoneal administrated to rat 1 h before RIR stimulation. Serum and pulmonary NO, MDA, IL-6, MIP-2, and PGE2 levels, arterial blood gas and biochemistry, lung wet/dry weight ratio and MPO activity, total cell number and protein concentration in BALF, tissue histology, and NF-κB expression were determined...
September 18, 2017: Inflammation
https://www.readbyqxmd.com/read/28920838/edaphobaculum-flavum-gen-nov-sp-nov-a-member-of-family-chitinophagaceae-isolated-from-grassland-soil
#16
Min Cao, Jing Huang, Jingxin Li, Zixu Qiao, Gejiao Wang
A yellow-coloured, Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterium, designated 1-116T, was isolated from Erdos grassland soil of Inner Mongolia, PR China. Phylogenetic analysis based on 16S rRNA genes showed that strain 1-116T was a member of family Chitinophagaceae and exhibited the highest similarities to Taibaiella koreensis THG-DT86T (90.1 %) and Flavihumibacter solisivae 3-3T (90.0 %), while the similarities to the other Chitinophagaceae type strains were lower than 90.0 %...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920759/spatial-distribution-of-polycyclic-aromatic-hydrocarbon-and-polychlorinated-biphenyl-sources-in-the-nakdong-river-estuary-south-korea
#17
Jun H Lee, Han J Woo, Kap S Jeong, Jeong W Kang, Jae U Choi, Eun J Jeong, Kap S Park, Dong H Lee
Our research team investigated the elemental composition and the presence of various toxic organic compounds, such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), in estuary surface sediments to trace the spatial distribution of the sources of pollution deposited in Nakdong River, Busan, South Korea. The spatial patterns of elemental composition and toxic organic compounds were determined from the measurements of total organic carbon (TOC), total nitrogen, total sulfur, PAHs, and PCBs...
September 18, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28920411/effect-of-inclusion-level-and-adaptation-duration-on-digestible-energy-and-nutrient-digestibility-in-palm-kernel-meal-fed-to-growing-finishing-pigs
#18
Chengfei Huang, Shuai Zhang, Hans-Henrik Stein, Jinbiao Zhao, Defa Li, Changhua Lai
Objective: An experiment was conducted to evaluate effects of inclusion level of palm kernel meal (PKM) and adaptation duration on the digestible energy (DE) and apparent total tract digestibility (ATTD) of chemical constituents in diets fed to growing-finishing pigs. Methods: Thirty crossbred barrows (Duroc × Landrace × Large White) with an average initial body weight (BW) of 85.0 ± 2.1 kg were fed to 5 diets in a completely randomized design. The diets included a corn-soybean meal basal diet and 4 additional diets in which corn and soybean meal (SBM) were partly replaced by 10, 20, 30, or 40% PKM...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28920400/-production-of-gastric-mucosa-protective-collagen-%C3%A2-by-pichia-pastoris
#19
Weina Li, Zifang Shang, Zhiguang Duan, Linbo Li, Jing He, Daidi Fan
To improve collagen production by recombinant Pichia pastoris, we applied Placket-Burman and Box-Behnken design to optimize the fermentation medium. Through Placket-Burman design, three variables in the medium (concentration of yeast extract, peptone and glycerol) were selected for having significant effect on cell dry weight. Through Box-Behnken design regression coefficients analysis, a secondary degree polynomial equation was established, and the optimum levels of the three variables were yeast extract 1...
April 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28919904/plasticity-of-sorghum-stem-biomass-accumulation-in-response-to-water-deficit-a-multiscale-analysis-from-internode-tissue-to-plant-level
#20
Lisa Perrier, Lauriane Rouan, Sylvie Jaffuel, Anne Clément-Vidal, Sandrine Roques, Armelle Soutiras, Christelle Baptiste, Denis Bastianelli, Denis Fabre, Cécile Dubois, David Pot, Delphine Luquet
Sorghum is increasingly used as a biomass crop worldwide. Its genetic diversity provides a large range of stem biochemical composition suitable for various end-uses as bioenergy or forage. Its drought tolerance enables it to reasonably sustain biomass production under water limited conditions. However, drought effect on the accumulation of sorghum stem biomass remains poorly understood which limits progress in crop improvement and management. This study aimed at identifying the morphological, biochemical and histological traits underlying biomass accumulation in the sorghum stem and its plasticity in response to water deficit...
2017: Frontiers in Plant Science
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