Fernando Lopes, Åsa V Keita, Alpana Saxena, Jose Luis Reyes, Nicole L Mancini, Ala Al Rajabi, Arthur Wang, Cristiane H Baggio, Michael Dicay, Rob van Dalen, Younghee Ahn, Matheus B H Carneiro, Nathan C Peters, Jong M Rho, Wallace K MacNaughton, Stephen E Girardin, Humberto Jijon, Dana J Philpott, Johan D Söderholm, Derek M McKay
The gut microbiome contributes to inflammatory bowel disease (IBD), in which bacteria can be present within the epithelium. Epithelial barrier function is decreased in IBD, and dysfunctional epithelial mitochondria and endoplasmic reticulum (ER) stress have been individually associated with IBD. We therefore hypothesized that the combination of ER and mitochondrial stresses significantly disrupt epithelial barrier function. Here, we treated human colonic biopsies, epithelial colonoids, and epithelial cells with an uncoupler of oxidative phosphorylation, dinitrophenol (DNP), with or without the ER stressor tunicamycin and assessed epithelial barrier function by monitoring internalization and translocation of commensal bacteria...
March 2, 2018: Journal of Biological Chemistry