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Orthopedic stem cell

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https://www.readbyqxmd.com/read/29228886/continuous-stimulation-with-differentiation-factors-is-necessary-to-enhance-osteogenic-differentiation-of-human-mesenchymal-stem-cells-in-vitro
#1
Bruno Reible, Gerhard Schmidmaier, Matthäus Prokscha, Arash Moghaddam, Fabian Westhauser
Bone defect treatment belongs to the most challenging fields in orthopedic surgery and requires the well-coordinated application of mesenchymal stem cells (MSC) and differentiation factors. MSC isolated from reaming material (RMSC) and iliac crest (BMSC) in combination with bone morphogenetic protein-7 (BMP-7) and insulin-like growth factor-1 (IGF-1) have been used. The short half-life of both factors limit their applications: a burst release of the factor can probably not induce sustainable differentiation...
October 2017: Growth Factors
https://www.readbyqxmd.com/read/29208555/hollow-mesoporous-zirconia-delivery-system-for-biomineralization-precursors
#2
Xue-Qing Huang, Hong-Ye Yang, Tao Luo, Cui Huang, Franklin R Tay, Li-Na Niu
Strategies based on the combination of nanocarrier delivery systems and scaffolds provide bone tissue engineering scaffolds with multifunctional capability. Zirconia, a biocompatible ceramic commonly used in orthopedic and dental implants, was used to synthesize hollow mesoporous nanocapsules for loading, storage and sustained release of a novel polyamine-stabilized liquid precursor phase of amorphous calcium phosphate (PAH-ACP) for collagen biomineralization and bone marrow stromal cells osteoinduction. Hollow mesoporous zirconia (hmZrO2) nanocapsules loaded with biomimetic precursors exhibited pH-sensitive release capability and good biocompatibility...
December 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29201060/osteogenic-differentiation-of-hdpscs-on-biogenic-bone-apatite-thin-films
#3
Michele Bianchi, Alessandra Pisciotta, Laura Bertoni, Matteo Berni, Alessandro Gambardella, Andrea Visani, Alessandro Russo, Anto de Pol, Gianluca Carnevale
A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29156291/engineering-the-next-generation-tin-containing-%C3%AE-titanium-alloys-with-high-strength-and-low-modulus-for-orthopedic-applications
#4
Sumit Bahl, Suvam Das, Satyam Suwas, Kaushik Chatterjee
Metastable β Ti alloys are the new emerging class of biomaterial for load bearing orthopedic applications. However, these alloys in the single β phase microstructure have insufficient strength for use in load bearing applications. It is imperative to strengthen these alloys by carefully designed thermo-mechanical processing routes that typically involve aging treatment. In this investigation two newly designed Sn based β Ti alloys of composition Ti-32Nb-(2, 4) Sn are evaluated. The effects of Sn content on the mechanical properties and biological performance of these alloys processed through designed thermo-mechanical processing route are investigated...
November 11, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29140074/facile-and-versatile-strategy-for-construction-of-anti-inflammatory-and-antibacterial-surfaces-with-polydopamine-mediated-liposomes-releasing-dexamethasone-and-minocycline-for-potential-implants-applications
#5
Xiao Xu, Lixin Wang, Zuyuan Luo, Yaofeng Ni, Haitao Sun, Xiang Gao, Yongliang Li, Siqi Zhang, Yan Li, Shicheng Wei
Reducing early nonbacterial inflammation induced by implanted materials and infection resulting from bacterial contamination around the implant-abutment interface could greatly accelerate implant osseointegration and reduce implant failure, which would be of clinical significance. In this study, we developed a facile and versatile strategy for the construction of anti-inflammatory and antibacterial surfaces on implants, such as orthopedic/dental implants. Briefly, the surfaces of polystyrene (PS) culture plates were first coated with polydopamine and then decorated with dexamethasone plus minocycline-loaded liposomes (Dex/Mino liposomes), which was confirmed by contact angle goniometry, X-ray photoelectron spectroscopy, and fluorescence microscopy...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29126349/bone-marrow-aspirate-concentrate-for-cartilage-defects-of-the-knee-from-bench-to-bedside-evidence
#6
Eric J Cotter, Kevin C Wang, Adam B Yanke, Susan Chubinskaya
Objective To critically evaluate the current basic science, translational, and clinical data regarding bone marrow aspirate concentrate (BMAC) in the setting of focal cartilage defects of the knee and describe clinical indications and future research questions surrounding the clinical utility of BMAC for treatment of these lesions. Design A literature search was performed using the PubMed and Ovid MEDLINE databases for studies in English (1980-2017) using keywords, including ["bone marrow aspirate" and "cartilage"], ["mesenchymal stem cells" and "cartilage"], and ["bone marrow aspirate" and "mesenchymal stem cells" and "orthopedics"]...
November 1, 2017: Cartilage
https://www.readbyqxmd.com/read/29118579/surface-modification-of-vascular-endothelial-growth-factor-loaded-silk-fibroin-to-improve-biological-performance-of-ultra-high-molecular-weight-polyethylene-via-promoting-angiogenesis
#7
Chengchong Ai, Dandan Sheng, Jun Chen, Jiangyu Cai, Siheng Wang, Jia Jiang, Shiyi Chen
Ultra-high-molecular-weight polyethylene (UHMWPE) has been applied in orthopedics, as the materials of joint prosthesis, artificial ligaments, and sutures due to its advantages such as high tensile strength, good wear resistance, and chemical stability. However, postoperative osteolysis induced by UHMWPE wear particles and poor bone-implant healing interface due to scarcity of osseointegration is a significant problem and should be solved imperatively. In order to enhance its affinity to bone tissue, vascular endothelial growth factor (VEGF) was loaded on the surface of materials, the loading was performed by silk fibroin (SF) coating to achieve a controlled-release delivery...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29100398/tenomodulin-highly-expressing-mscs-as-a-better-cell-source-for-tendon-injury-healing
#8
Yonghui Hou, Ming Ni, Sien Lin, Yuxin Sun, Weiping Lin, Yamei Liu, Haibin Wang, Wei He, Gang Li, Liangliang Xu
Tendon injuries are common orthopedic problems which may cause severe morbidity. MSCs (mesenchymal stem cells) have shown promising effect on tissue engineering and have been used for the treatment of tendon injury. But the low tenogenic differentiation capacity of MSCs have hindered their application. In the present study, we have constructed the Tenomodulin (Tnmd) promoter-driven GFP expression lentiviral plasmid. After transduced into BMSCs, the expression of GFP was used to select BMSCs highly expressing Tnmd by flow cytometry...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29098319/-multiple-myeloma-what-has-been-confirmed-in-therapy
#9
REVIEW
M-A Baertsch, H Goldschmidt
Multiple myeloma (MM) is a malignancy of terminally differentiated B cells/plasma cells and is primarily located in the bone marrow. Symptomatic multiple myeloma typically presents with osteolyses, anemia, reduced renal function, and/or hypercalcemia. In the case of such MM-related end organ damage, urgent systemic treatment is indicated. In order to prevent end organ damage, current guidelines now recommend treatment initiation already when certain biomarkers are met. Current first-line treatment is based on proteasome inhibition and immunomodulation...
November 2, 2017: Der Internist
https://www.readbyqxmd.com/read/29079514/dual-modulation-of-bone-formation-and-resorption-with-zoledronic-acid-loaded-biodegradable-magnesium-alloy-implants-improves-osteoporotic-fracture-healing-an-in-vitro-and-in-vivo-study
#10
Guoyuan Li, Lei Zhang, Lei Wang, Guangyin Yuan, Kerong Dai, Jia Pei, Yongqiang Hao
Osteoporotic fracture (OPF) remains a major clinical challenge for skeletal regeneration. Impaired osteogenesis and excessive remodeling result in prolonged and poor quality of fracture healing. To augment bone formation and inhibit excessive resorption simultaneously, we constructed a biodegradable magnesium-based implant integrated with the anti-catabolic drug zoledronic acid (ZA); this implant exhibits controllable, sustained release of magnesium degradation products and ZA in vitro. The extracts greatly stimulate the osteogenic differentiation of rat-bone marrow-derived mesenchymal stem cells (rBMSCs), while osteoclastogenesis is inhibited by ZA...
October 24, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29076267/concise-review-multifaceted-characterization-of-human-mesenchymal-stem-cells-for-use-in-regenerative-medicine
#11
REVIEW
Rebekah M Samsonraj, Michael Raghunath, Victor Nurcombe, James H Hui, Andre J van Wijnen, Simon M Cool
Mesenchymal stem cells (MSC) hold great potential for regenerative medicine because of their ability for self-renewal and differentiation into tissue-specific cells such as osteoblasts, chondrocytes, and adipocytes. MSCs orchestrate tissue development, maintenance and repair, and are useful for musculoskeletal regenerative therapies to treat age-related orthopedic degenerative diseases and other clinical conditions. Importantly, MSCs produce secretory factors that play critical roles in tissue repair that support both engraftment and trophic functions (autocrine and paracrine)...
October 26, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29038672/safety-analysis-of-autologous-stem-cell-therapy-in-a-variety-of-degenerative-diseases-and-injuries-using-the-stromal-vascular-fraction
#12
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29033930/immunomodulatory-effects-of-calcium-and-strontium-co-doped-titanium-oxides-on-osteogenesis
#13
Xiangwei Yuan, Huiliang Cao, Jiaxing Wang, Kaiwei Tang, Bin Li, Yaochao Zhao, Mengqi Cheng, Hui Qin, Xuanyong Liu, Xianlong Zhang
The effects of calcium (Ca) or strontium (Sr) on host osteogenesis and immune responses have been investigated separately. In clinical practice, these two elements may both be present around an orthopedic device, but their potential synergistic effects on osteogenesis and the immune response have not been explored to date. In this work, we investigated the immunomodulatory effects of Ca and Sr co-doped titanium oxides on osteogenesis in vitro using the mouse macrophage cell line RAW 264.7 alone and in co-culture with mouse bone mesenchymal stem cells (BMSCs), and in vivo using a mouse air-pouch model...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28986619/-therapeutic-utilization-of-stem-cells-in-orthopedics
#14
C Chiari, S Walzer, D Stelzeneder, M Schreiner, R Windhager
Stem cells are becoming increasingly more important in the field of regenerative medicine. Adult mesenchymal stem cells (MSCs) are harvested predominantly from bone marrow or adipose tissue, are already being used in the clinical setting and have a low potential for side effects. In orthopedics, experience has been gained in the treatment of bone defects, non-unions, cartilage defects, osteoarthritis and tendon pathologies. The current data are derived from case studies and randomized controlled trials are missing; therefore, there are many open questions concerning the optimal cell source, number of cells, administration technique (e...
October 6, 2017: Der Orthopäde
https://www.readbyqxmd.com/read/28891421/bone-marrow-aspiration-for-regenerative-orthopedic-intervention-technique-with-ultrasound-guidance-for-needle-placement
#15
Shane A Shapiro, Jennifer R Arthurs
AIM: We review relevant anatomy of the iliac crest, and describe an interventional technique to maximize harvesting of desired progenitor cells with ultrasound to guide safe trochar placement. MATERIALS & METHODS: We validated the technique on both sides of the pelvis in four human cadavers. RESULTS: Using ultrasound guidance, 32 BMA needles were placed in a safe zone along various portions of the iliac crest. CONCLUSION: Ultrasound guidance can improve accuracy of bone marrow aspirations form the iliac crest...
September 11, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28879686/development-of-a-bioactive-porous-collagen-%C3%AE-tricalcium-phosphate-bone-graft-assisting-rapid-vascularization-for-bone-tissue-engineering-applications
#16
Nafiseh Baheiraei, Mohamma Reza Nourani, Sm Javad Mortazavi, Mansoureh Movahedin, Hossein Eyni, Fatemeh Baghery, Mohammad Hadi Norahan
We developed collagen (COL) and collagen/beta tricalcium phosphate (COL/β-TCP) scaffolds with a β-TCP/collagen weight ratio of 4 by freeze-drying. Mouse bone marrow-derived mesenchymal stem cells (BMMSCs) were cultured on these scaffolds for 14 days. Samples were characterized by physicochemical analyses and their biological properties such as cell viability and alkaline phosphatase (ALP) activity was,also, examined. Additionally, the vascularization potential of the prepared scaffolds was tested subcutaneously in Wistar rats...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28865450/elution-kinetics-of-vancomycin-and-gentamicin-from-carriers-and-their-effects-on-mesenchymal-stem-cell-proliferation-an-in-vitro-study
#17
Tomas Kucera, Lenka Ryskova, Tomas Soukup, Jana Malakova, Eva Cermakova, Pavel Mericka, Jakub Suchanek, Pavel Sponer
BACKGROUND: Musculoskeletal infections remain a major complication in orthopedic surgery. The local delivery of antibiotics provides the high levels required to treat an infection without systemic toxicity. However, the local toxicity of antibiotic carriers to the mesenchymal stem cells, as a result of both the peak concentrations and the type of carrier, may be significant. METHODS: To address this concern, the elution kinetics of vancomycin and gentamicin from several commercially available antibiotic carriers and several carriers impregnated by a surgeon (10 ml of each sterile carrier were manually mixed with a 500 mg vancomycin and an 80 mg gentamicin solution, and the duration of impregnation was 30 min) were assessed...
September 2, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28844671/transcriptional-profiling-of-human-femoral-mesenchymal-stem-cells-in-osteoporosis-and-its-association-with-adipogenesis
#18
Yong Jun Choi, Insun Song, Yilan Jin, Hyun-Seok Jin, Hyung Min Ji, Seon-Yong Jeong, Ye-Yeon Won, Yoon-Sok Chung
Genetic alterations are major contributing factors in the development of osteoporosis. Osteoblasts and adipocytes share a common origin, mesenchymal stem cells (MSCs), and their genetic determinants might be important in the relationship between osteoporosis and obesity. In the present study, we aimed to isolate differentially expressed genes (DEGs) in osteoporosis and normal controls using human MSCs, and elucidate the common pathways and genes related to osteoporosis and adipogenesis. Human MSCs were obtained from the bone marrow of femurs from postmenopausal women during orthopedic surgeries...
October 20, 2017: Gene
https://www.readbyqxmd.com/read/28818783/development-of-a-porous-3d-graphene-pdms-scaffold-for-improved-osseointegration
#19
Jianfeng Li, Xiao Liu, Jeremy M Crook, Gordon G Wallace
Osseointegration in orthopedic surgery plays an important role for bone implantation success. Traditional treatment of implant surface aimed at improved osseointegration has limited capability for its poor performance in supporting cell growth and proliferation. Polydimethylsiloxane (PDMS) is a widely used silicon-based organic polymer material with properties that are useful in cosmetics, domestic applications and mechanical engineering. In addition, the biocompatibility of PDMS, in part due to the high solubility of oxygen, makes it an ideal material for cell-based implants...
August 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28814956/rapid-rapamycin-only-induced-osteogenic-differentiation-of-blood-derived-stem-cells-and-their-adhesion-to-natural-and-artificial-scaffolds
#20
Carpentieri Arianna, Cozzoli Eliana, Acri Flavio, Ranalli Marco, Diedenhofen Giacomo, Scimeca Manuel, Bonanno Elena, Gambacurta Alessandra
Stem cells are a centerpiece of regenerative medicine research, and the recent development of adult stem cell-based therapy systems has vigorously expanded the scope and depth of this scientific field. The regeneration of damaged and/or degraded bone tissue in orthopedic, dental, or maxillofacial surgery is one of the main areas where stem cells and their regenerative potential could be used successfully, requiring tissue engineering solutions incorporating an ideal stem cell type paired with the correct mechanical support...
2017: Stem Cells International
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