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Orthopedic stem cell

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https://www.readbyqxmd.com/read/29666653/mesenchymal-stem-cells-in-oriented-plga-acecm-composite-scaffolds-enhance-structure-specific-regeneration-of-hyaline-cartilage-in-a-rabbit-model
#1
Weimin Guo, Xifu Zheng, Weiguo Zhang, Mingxue Chen, Zhenyong Wang, Chunxiang Hao, Jingxiang Huang, Zhiguo Yuan, Yu Zhang, Mingjie Wang, Jiang Peng, Aiyuan Wang, Yu Wang, Xiang Sui, Wenjing Xu, Shuyun Liu, Shibi Lu, Quanyi Guo
Articular cartilage lacks a blood supply and nerves. Hence, articular cartilage regeneration remains a major challenge in orthopedics. Decellularized extracellular matrix- (ECM-) based strategies have recently received particular attention. The structure of native cartilage exhibits complex zonal heterogeneity. Specifically, the development of a tissue-engineered scaffold mimicking the aligned structure of native cartilage would be of great utility in terms of cartilage regeneration. Previously, we fabricated oriented PLGA/ACECM (natural, nanofibrous, articular cartilage ECM) composite scaffolds...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29664925/actin-and-myosin-ii-modulate-differentiation-of-pluripotent-stem-cells
#2
Liana C Boraas, Emma T Pineda, Tabassum Ahsan
Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differentiation is known to be regulated by numerous extracellular and intracellular factors, but the role of the cytoskeleton during specification, or early differentiation, is still unknown. In these studies, we used agonists and antagonists to modulate actin polymerization and the actin-myosin molecular motor during spontaneous differentiation of embryonic stem cells in embryoid bodies...
2018: PloS One
https://www.readbyqxmd.com/read/29662652/guided-bone-regeneration-using-a-bone-tissue-engineering-complex-consisting-of-a-poly-dl-lactide-membrane-and-bone-mesenchymal-stem-cells
#3
Dahui Wang, Yifeng Lin, Lian Chen, Yueqiang Mo, Peng Huang, Ruixue Ma
Developmental dysplasia of the hip (DDH) is one of the most common diseases encountered in pediatric orthopedic departments. Current treatment strategies seek to improve acetabular coverage, the principal defect of acetabular dysplasia, but are not very successful. We developed a guided bone regeneration (GBR) strategy to improve acetabular coverage via bone tissue engineering (BTE). Poly- dl -lactide (PDLLA) membranes were seeded with bone marrow mesenchymal stem cells (BMSCs) to form a BTE complex, which was then implanted into the superior margin of the acetabulum in a rabbit DDH model...
March 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29657507/initial-response-of-human-bone-marrow-derived-stem-cells-after-contact-with-ultrahigh-molecular-weight-polyethylene-uhmwpe-material-an-in-vitro-study-on-cell-viability-and-interleukin-6-expression
#4
Iwan B Anwar, Asep Santoso, Eko Saputra, Rifky Ismail, J Jamari, Emile van der Heide
Introduction: Ultrahigh-molecular-weight polyethylene (UHMWPE) is a thermoplastic polymer useful in biomaterial applications, especially in orthopedic field. Yet, little is known concerning its initial effect on human bone marrow stem cells (hBMSCs) after implantation. Materials and Methods: A cytotoxicity analysis was performed with a 3-(4,5-dimethylthiazol 2-yl)-2,5-diphenyltetrazolium assay after 24, 48, and 72h of incubation of hBMSC culture. Expression of interleukin-6 (IL-6) was measured using enzyme-linked immunosorbent assay...
January 2018: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/29652173/a-review-of-commercially-available-point-of-care-devices-to-concentrate-bone-marrow-for-the-treatment-of-osteoarthritis-and-focal-cartilage-lesions
#5
Florian Gaul, William D Bugbee, Heinz R Hoenecke, Darryl D D'Lima
Objective Mesenchymal stem cells (MSCs) are a promising cell-based therapy treatment option for several orthopedic indications. Because culture expansion of MSC is time and cost intensive, a bedside concentration of bone marrow (BM) aspirate is used as an alternative. Many commercial systems are available but the available literature and knowledge regarding these systems is limited. We compared different point-of-care devices that concentrate BM (BMC) by focusing on technical features and quality parameters to help surgeons make informed decisions while selecting the appropriate device...
April 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29628701/management-of-growth-arrest-current-practice-and-future-directions
#6
REVIEW
Sherif Dabash, Gautham Prabhakar, Eric Potter, Ahmed M Thabet, Amr Abdelgawad, Stephen Heinrich
Introduction: Premature growth arrest can pose a challenge to the orthopedic surgeon. Various options for treating physeal arrest exist. Methods: Systematic searches were conducted on PubMed/Medline, ScienceDirect, OVID, and Cochrane Library. Secondary searching was performed, where certain articles from reference lists of the selected studies were reviewed that were not found in the primary search. Results: This review article discusses the different methods of management for premature growth arrest...
March 2018: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/29626698/in-vitro-evaluation-of-mgsr-and-mgcasr-alloys-via-direct-culture-with-bone-marrow-derived-mesenchymal-stem-cells
#7
Wensen Jiang, Aaron F Cipriano, Qiaomu Tian, Chaoxing Zhang, Marisa Lopez, Amy Sallee, Alan Lin, Mayra Celene Cortez Alcaraz, Yuanhao Wu, Yufeng Zheng, Huinan Liu
Magnesium (Mg) and its alloys have been widely investigated as the most promising biodegradable metals to replace conventional non-degradable metals for temporary medical implant applications. New Mg alloys have been developed for medical applications in recent years; and the concept of alloying Mg with less-toxic elements have aroused tremendous interests due to the promise to address the problems associated with rapid degradation of Mg without compromising its cytocompatibility and biocompatibility. Of particular interests for orthopedic/spinal implant applications are the additions of calcium (Ca) and strontium (Sr) into Mg matrix because of their beneficial properties for bone regeneration...
April 4, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29590087/microrna-expression-profiling-of-bone-marrow-mesenchymal-stem-cells-in-steroid-induced-osteonecrosis-of-the-femoral-head-associated-with-osteogenesis
#8
Ao Wang, Ming Ren, Yang Song, Xiaonan Wang, Qingyu Wang, Qiwei Yang, He Liu, Zhenwu Du, Guizhen Zhang, Jincheng Wang
BACKGROUND Steroid-induced osteonecrosis of the femoral head (SONFH) is a common orthopedic disease associated with the application of glucocorticoid (GC). In this study, we detected the microRNAs (miRNAs) differentially expressed in bone marrow mesenchymal stem cells (BMSCs) from SONFH patients, and target gene predictions were performed, and the functions of the target genes was verified. MATERIAL AND METHODS BMSCs collected from patients with SONFH and femoral neck fracture (FNF) constituted the SONFH group (n=3) and FNF (control) group (n=3), respectively...
March 28, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29580978/sheep-as-a-model-for-evaluating-mesenchymal-stem-stromal-cell-msc-based-chondral-defect-repair
#9
REVIEW
Ena Music, Kathryn Futrega, Michael R Doran
Osteoarthritis results from the degradation of articular cartilage and is one of the leading global causes of pain and immobility. Cartilage has a limited capacity for self-repair. While repair can be enhanced through surgical intervention, current methods often generate inferior fibrocartilage and repair is transient. The development of tissue engineering strategies to improve repair outcomes is an active area of research. While small animal models such as rodents and rabbits are often used in early pre-clinical work, larger animals that better recapitulate the anatomy and loading of the human joint are required for late-stage preclinical evaluation...
March 23, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29576458/encapsulation-of-mesenchymal-stem-cells-in-chitosan-%C3%AE-glycerophosphate-hydrogel-for-seeding-on-a-novel-calcium-phosphate-cement-scaffold
#10
Tao Liu, Jian Li, Zengwu Shao, Kaige Ma, Zhicai Zhang, Baichuan Wang, Yannan Zhang
Due to its moldability, biocompatibility, osteoconductivity and resorbability, calcium phosphate cement (CPC) is a highly promising scaffold material for orthopedic applications. However, pH changes and ionic activity during the CPC setting reaction may adversely affect cells seeded directly on CPC. Moreover, a lack of macropores in CPC limits ingrowth of new bone. The objectives of this study were to prepare macroporous CPC scaffolds via porogen leaching, using mannitol crystals as the porogen and to evaluate the in vitro proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) encapsulated in chitosan/β-glycerophosphate (C/GP) hydrogel prior to exposure to the novel CPC scaffold...
March 22, 2018: Medical Engineering & Physics
https://www.readbyqxmd.com/read/29569344/application-of-alginate-microbeads-as-a-carrier-of-bone-morphogenetic-protein-2-for-bone-regeneration
#11
Yun Hwan Lee, Byung-Woo Lee, Yun Chan Jung, Byung-Il Yoon, Heung-Myong Woo, Byung-Jae Kang
Bone morphogenetic protein-2 (BMP-2) is commonly used to enhance bone regeneration. The potential of BMP-2 for bone regeneration varies according to the concentration and release kinetics on the implanted site. Therefore, it is important to determine appropriate carriers of BMP-2. However, no optimal delivery vehicles have been identified. In the present study, we used alginate microbeads as a delivery vehicle for BMP-2. Alginate microbeads can be implanted onto the disease site through surgery or injection...
March 23, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29568314/platelet-lysate-induces-chondrogenic-differentiation-of-umbilical-cord-derived-mesenchymal-stem-cells
#12
Ghmkin Hassan, Mohammad Bahjat, Issam Kasem, Chadi Soukkarieh, Majd Aljamali
Purpose: Articular cartilage has a poor capacity for self-repair, and thus still presents a major challenge in orthopedics. Mesenchymal stem cells (MSCs) are multipotent stem cells with the potential to differentiate into chondrocytes in the presence of transforming growth factor beta (TGF-β). Platelet lysate (PL) contains a relatively large number of growth factors, including TGF-β, and has been shown to ameliorate cartilage repair. Here, we investigated the ability of PL to direct chondrogenic differentiation of MSCs along with other standard differentiation components in a pellet culture system...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29554720/infrapatellar-fat-pad-derived-stem-cell-based-regenerative-strategies-in-orthopedic-surgery
#13
REVIEW
Pinar Yilgor Huri, Seren Hamsici, Emre Ergene, Gazi Huri, Mahmut Nedim Doral
Infrapatellar fat pad is a densely vascularized and innervated extrasynovial tissue that fills the anterior knee compartment. It plays a role in knee biomechanics as well as constitutes a source of stem cells for regeneration after knee injury. Infrapatellar fat pad-derived stem cells (IPFP-ASCs) possess enhanced and age-independent differentiation capacity as compared to other stem cells, which makes them a very promising candidate in stem cell-based regenerative therapy. The aims of this review are to outline the latest advances and potential trends in using IPFP-ASCs and to emphasize the advantages over other sources of stem cells for applications in orthopedic surgery...
March 19, 2018: Knee Surgery & related Research
https://www.readbyqxmd.com/read/29547701/mangiferin-enhances-endochondral-ossification-based-bone-repair-in-massive-bone-defect-by-inducing-autophagy-through-activating-amp-activated-protein-kinase-signaling-pathway
#14
Yun Bai, Chuan Liu, Lei Fu, Xiaoshan Gong, Ce Dou, Zhen Cao, Hongyu Quan, Jianmei Li, Fei Kang, Jingjin Dai, Chunrong Zhao, Shiwu Dong
Endochondral ossification is crucial for bone formation in both adult bone repair process and embryo long-bone development. In endochondral ossification, bone marrow-derived mesenchymal stem cells (BMSCs) first differentiate to chondrocyte, BMSC-derived chondrocyte then endure a hypertrophic process to generate new bone. Endochondral ossification-based bone repair is a promising strategy to cure massive bone defect, which is a major clinical issue in orthopedics. However, challenges still remain for this novel strategy...
March 16, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29529506/modulation-of-osteogenic-and-haemostatic-activities-by-tuning-cationicity-of-genipin-crosslinked-chitosan-hydrogels
#15
Nihui Zhang, Ruijuan Yao, Jianglong Guo, Jing He, Guolong Meng, Fang Wu
Chitosan as a natural cationic polysaccharide has drawn wide interests as surface modification materials in orthopedic applications, with the potential to achieve combined osteogenic, antimicrobial and haemostatic functions. The cationicity of chitosan has been reported to play an important role in modifying the osteoblastic cell responses and the antibacterial activities, while its effect on the haemostatic properties has been rarely studied. To this aim, we prepared carboxymethyl chitosan hydrogels with different cationicity through crosslinking with different concentrations of genipin (1%, 2...
February 25, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29523197/radial-shockwave-treatment-promotes-human-mesenchymal-stem-cell-self-renewal-and-enhances-cartilage-healing
#16
Hao Zhang, Zhong-Li Li, Fei Yang, Qiang Zhang, Xiang-Zheng Su, Ji Li, Ning Zhang, Chun-Hui Liu, Ning Mao, Heng Zhu
BACKGROUND: Shockwaves and mesenchymal stem cells (MSCs) have been widely accepted as useful tools for many orthopedic applications. However, the modulatory effects of shockwaves on MSCs remain controversial. In this study, we explored the influence of radial shockwaves on human bone marrow MSCs using a floating model in vitro and evaluated the healing effects of these cells on cartilage defects in vivo using a rabbit model. METHODS: MSCs were cultured in vitro, harvested, resuspended, and treated with various doses of radial shockwaves in a floating system...
March 9, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29484201/differentiated-osteoblasts-derived-decellularized-extracellular-matrix-to-promote-osteogenic-differentiation
#17
Jin Jeon, Min Suk Lee, Hee Seok Yang
Background: The extracellular matrix (ECM) can directly or indirectly influence on regulation of cell functions such as cell adhesion, migration, proliferation and differentiation. The cell derived ECM (CD-ECM) is a useful in vitro model for studying the comprehensive functions of CD-ECM because it maintains a native-like structure and composition. In this study, the CD-ECM is obtained and a test is carried out to determine the effectiveness of several combinations of decellularized methods...
2018: Biomaterials Research
https://www.readbyqxmd.com/read/29478615/neurofibromatosis-type-1
#18
Patrick J Cimino, David H Gutmann
The neurofibromatoses are a group of three heterogeneous disorders that include neurofibromatosis type 1 (NF1), neurofibromatosis type 2 (NF2), and schwannomatosis. NF1 is the most common of these three conditions, and represents one of the most frequently diagnosed cancer predisposition disorders involving the nervous system. While NF1 primarily affects the central and peripheral nervous system, multisystem involvement is the rule, with dermatologic, cardiovascular, gastrointestinal, and orthopedic affectation often reported...
2018: Handbook of Clinical Neurology
https://www.readbyqxmd.com/read/29468802/synergistic-effect-of-strontium-bioactive-glass-and-nano-hydroxyapatite-promotes-bone-regeneration-of-critical-sized-radial-bone-defects
#19
Ahmad Oryan, Mohamadreza Baghaban Eslaminejad, Amir Kamali, Samaneh Hosseini, Forough Azam Sayahpour, Hossein Baharvand
Critical-sized bone defects constitute a major health issue in orthopedics and usually cause mal-unions due to an inadequate number of migrated progenitor cells into the defect site or their incomplete differentiation into osteogenic precursor cells. The current study aimed to develop an optimized osteoinductive and angiogenic scaffold by incorporation of strontium (Sr) and bioglass (BG) into gelatin/nano-hydroxyapatite (G/nHAp) seeded with bone marrow mesenchymal stem cells to enhance bone regeneration. The scaffolds were fabricated by a freeze-drying technique and characterized in terms of morphology, structure, porosity and degradation rate...
February 21, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29464750/inhibition-of-adam10-in-satellite-cells-accelerates-muscle-regeneration-following-muscle-injury
#20
Sakiko Mizuno, Masaki Yoda, Masayuki Shimoda, Kazuhiro Chiba, Masaya Nakamura, Keisuke Horiuchi
Muscle injury is one of the most common orthopedic and sports disorders. For severe cases, surgical repair may be indicated; however, other than immobilization and the administration of anti-inflammatory drugs there is currently no effective conservative treatment for this condition. Satellite cells (SCs) are muscle-specific stem cells and are indispensable for muscle regeneration after muscle injury. SCs are activated upon muscle injury to proliferate and differentiate into myoblasts, which subsequently fuse into myofibers and regenerate the damaged muscle...
February 21, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
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