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Orthopedic stem cell

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https://www.readbyqxmd.com/read/29906095/strontium-doped-bioactive-glass-nanoparticles-in-osteogenic-commitment
#1
Alvaro J Leite, Ana I Gonçalves, Marcia T Rodrigues, Manuela E Gomes, João F Mano
The present work explores bioactive glass nanoparticles (BGNPs) and developed strontium-doped nanoparticles (BGNPsSr), envisioning orthopedic strategies compatible with vascularization. The nanoparticles were synthesized by sol-gel, achieving a diameter of 55 nm for BGNPs and of 75 nm for BGNPsSr, and the inclusion of strontium caused none structural alteration. The nanoparticles exhibited high cytocompatibility for HUVECs and SaOS-2. Additionally, the incorporation of strontium emphasized the tubule networking behavior of HUVECs...
June 15, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29900517/mesenchymal-stem-cells-in-osteotomy-repair-after-tibial-tuberosity-advancement-in-dogs-with-cranial-cruciate-ligament-injury
#2
Clarissa Rocha Dos Santos, Richard da Rocha Filgueiras, Patrícia Furtado Malard, Andre Rodrigues da Cunha Barreto-Vianna, Kaique Nogueira, Carolina da Silva Leite, Eduardo Maurício Mendes de Lima
BACKGROUND: The cranial cruciate ligament rupture (CCLR) is the most commonly encountered orthopedic condition in dogs. Among the various techniques to treat this condition, tibial tuberosity advancement (TTA) has been used to obtain rapid recovery of the affected knee. The objective of this study was to evaluate the viability of the use of mesenchymal stem cells (MSC) implanted in the osteotomy site obtained by TTA in nine dogs diagnosed with CCLR. METHODS: The MSC were isolated from the adipose tissue of the dogs and cultured for eight days, the animals were divided into two groups...
June 14, 2018: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/29897021/biomaterials-enabled-cell-free-strategies-for-endogenous-bone-regeneration
#3
Xiaojing Wang, Guowei Wang, Sarah Zingales, Baodong Zhao
Repairing bone defects poses a major orthopedic challenge because current treatments are constrained by the limited regenerative capacity of human bone tissue. Novel therapeutic strategies, such as stem cell therapy and tissue engineering, have the potential to enhance bone healing and regeneration, and hence may improve quality of life for millions of people. However, the ex vivo expansion of stem cells and their in vivo delivery pose technical difficulties that hamper clinical translation and commercial development...
June 13, 2018: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/29862995/an-update-tissue-engineered-nerve-grafts-for-the-repair-of-peripheral-nerve-injuries
#4
Nitesh P Patel, Kristopher A Lyon, Jason H Huang
Peripheral nerve injuries (PNI) are caused by a range of etiologies and result in a broad spectrum of disability. While nerve autografts are the current gold standard for the reconstruction of extensive nerve damage, the limited supply of autologous nerve and complications associated with harvesting nerve from a second surgical site has driven groups from multiple disciplines, including biomedical engineering, neurosurgery, plastic surgery, and orthopedic surgery, to develop a suitable or superior alternative to autografting...
May 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29857818/development-of-a-novel-biomaterial-with-an-important-osteoinductive-capacity-for-hard-tissue-engineering
#5
Meda-Romana Simu, Emoke Pall, Teodora Radu, Maria Miclaus, Bogdan Culic, Anca-Stefania Mesaros, Alexandrina Muntean, Gabriela Adriana Filip
In this study we designed a composite biomaterial based on a high viscosity soft propolis extract (70% propolis) and shell clam, with antiseptic and osteoinductive qualities, that can be used in dentistry, orthopedics and other areas where hard tissue regeneration is needed. We assessed it in interaction with stabilized human cells isolated from dental papilla of wisdom teeth (D1MSCs). We performed detailed characterization of the obtained material by Scanning Electronic Microscopy (SEM), X-Ray Diffraction (XRD), Energy Dispersive X-Ray Spectroscopy (EDX), Fourier Transform Infrared Spectroscopy (FTIR) techniques...
June 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29803179/platelet-rich-plasma-stromal-vascular-fraction-and-autologous-conditioned-serum-in-treatment-of-knee-osteoarthritis
#6
REVIEW
Ali Fotouhi, Arash Maleki, Sanam Dolati, Ali Aghebati-Maleki, Leili Aghebati-Maleki
Osteoarthritis (OA) is a multifactorial chronic disease, causing several problems on patients, hygiene and community care systems. Conventional therapies, such as non-pharmacological mediations, systemic drug treatment and intra-articular therapies are applying previously; however, controlling and management approaches of the disease mainly remain insufficient. Injections of intra-articular therapies directly into the joint evade conservative obstacles to joint entry, rise bioavailability and minor systemic toxicity...
August 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29784586/ultrasound-mediated-gene-delivery-enhances-tendon-allograft-integration-in-mini-pig-ligament-reconstruction
#7
Maxim Bez, Thomas J Kremen, Wafa Tawackoli, Pablo Avalos, Dmitriy Sheyn, Galina Shapiro, Joseph C Giaconi, Shiran Ben David, Jess G Snedeker, Zulma Gazit, Katherine W Ferrara, Dan Gazit, Gadi Pelled
Ligament injuries occur frequently, substantially hindering routine daily activities and sports participation in patients. Surgical reconstruction using autogenous or allogeneic tissues is the gold standard treatment for ligament injuries. Although surgeons routinely perform ligament reconstructions, the integrity of these reconstructions largely depends on adequate biological healing of the interface between the ligament graft and the bone. We hypothesized that localized ultrasound-mediated, microbubble-enhanced therapeutic gene delivery to endogenous stem cells would lead to significantly improved ligament graft integration...
April 26, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29781148/improved-osteogenesis-of-boron-incorporated-calcium-silicate-coatings-via-immunomodulatory-effects
#8
Xiang Lu, Kai Li, Youtao Xie, Shengcai Qi, Qingyi Shen, Jiangming Yu, Liping Huang, Xuebin Zheng
Osteoimmunology has revealed the importance of a favorable immune response for successful biomaterial-mediated osteogenesis. Boron-incorporated calcium silicate (Ca11 Si4 B2 O22 , B-CS) coating has been reported as a potential candidate for improving osteogenesis in orthopedic applications in vitro. However, relatively little is known about its effects on the immune response and subsequent osteogenesis. In this work, the immunomodulatory properties of the B-CS coating and its specific mechanism of action were explored...
May 20, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29758548/stromal-cell-derived-factor-sdf-1-alpha-overexpression-promotes-bone-regeneration-by-osteogenesis-and-angiogenesis-in-osteonecrosis-of-the-femoral-head
#9
Fan Yang, Feng Xue, Junjie Guan, Zeng Zhang, Jimin Yin, Qingling Kang
BACKGROUND/AIMS: Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease. Previous studies suggested that stromal-cell-derived factor (SDF)-1 was involved in osteogenesis and angiogenesis. However, whether SDF-1 potentiates the angiogenesis and osteogenesis of bone marrow-derived stromal stem cells (BMSCs) in ONFH is not clear. METHODS: BMSCs were transfected with green fluorescent protein (GFP) or the fusion gene encoding GFP and SDF-1α, and transgenic efficacy was monitored by immunofluorescence...
May 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29736572/hyaluronic-acid
#10
Hélder Pereira, Duarte Andre Sousa, António Cunha, Renato Andrade, J Espregueira-Mendes, J Miguel Oliveira, Rui L Reis
In recent times, the field of tissue engineering and regenerative medicine (TERM) has considerably increased the extent of therapeutic strategies for clinical application in orthopedics. However, TERM approaches have its rules and requirements, in the respect of the biologic response of each tissue and bioactive agents which need to be considered, respected, and subject of ongoing studies. Different medical devices/products have been prematurely available on the market and used in clinics with limited success...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29736095/repair-of-critical-sized-rat-calvarial-defects-with-three-dimensional-hydroxyapatite-gelatin-scaffolds-and-bone-marrow-stromal-stem-cells
#11
Hatef Ghasemi Hamidabadi, Majid Malekzadeh Shafaroudi, Morteza Seifi, Maryam Nazm Bojnordi, Masume Behruzi, Mazaher Gholipourmalekabadi, Ali Malekzadeh Shafaroudi, Nourollah Rezaei
Introduction: The repair of critical-sized defects (CSDs) are one of the most challenging orthopedic problems and the attempts for development of an ideal scaffold for treatment of large bone defect are ongoing. Aim: The aim of this study was the effectiveness of hydroxyapatite-gelatin seeded with bone marrow stromal cells construct for healing of critical-sized bone defect in vivo. Material and Methods: In this experimental study, the bone marrow stromal cells (BMSCs) were isolated by flushing method...
April 2018: Medical Archives
https://www.readbyqxmd.com/read/29722767/amniotic-epithelial-stem-cells-salient-features-and-possible-therapeutic-role
#12
Aurelio Muttini, Barbara Barboni, Luca Valbonetti, Valentina Russo, Nicola Maffulli
This is a study of amniotic epithelial cells, which form the innermost layer of the amniotic membrane. These cells can be easily isolated and display peculiar and unique properties, such as plasticity and differentiation potential toward the 3 germinal layers, that may aid regeneration and/or repair of damaged or diseased tissues and organs. A robust literature based on in vitro, experimental, and clinical studies in large animals demonstrates that these cells can enhance the regeneration of tendons, bone, and articular cartilage...
June 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29719605/-in-vivo-safety-and-efficacy-testing-of-a-thermally-triggered-injectable-hydrogel-scaffold-for-bone-regeneration-and-augmentation-in-a-rat-model
#13
Abbey A Thorpe, Christine Freeman, Paula Farthing, Jill Callaghan, Paul V Hatton, Ian M Brook, Chris Sammon, Christine Lyn Le Maitre
Bone loss resulting from degenerative diseases and trauma is a significant clinical burden which is likely to grow exponentially with the aging population. In a number of conditions where pre-formed materials are clinically inappropriate an injectable bone forming hydrogel could be beneficial. The development of an injectable hydrogel to stimulate bone repair and regeneration would have broad clinical impact and economic benefit in a variety of orthopedic clinical applications. We have previously reported the development of a Laponite® crosslinked pNIPAM-co-DMAc (L-pNIPAM-co-DMAc) hydrogel delivery system, loaded with hydroxyapatite nanoparticles (HAPna), which was capable of inducing osteogenic differentiation of mesenchymal stem cells (MSCs) without the need for additional growth factors in vitro ...
April 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29702291/multilayered-coating-of-titanium-implants-promotes-coupled-osteogenesis-and-angiogenesis-in-vitro-and-in-vivo
#14
Weizhen Chen, Kui Xu, Bailong Tao, Liangliang Dai, Yonglin Yu, Caiyun Mu, Xinkun Shen, Yan Hu, Ye He, Kaiyong Cai
We used surface-modified titanium (Ti) substrates with a multilayered structure composed of chitosan-catechol (Chi-C), gelatin (Gel) and hydroxyapatite (HA) nanofibers, which were previously shown to improve osteogenesis, as a platform to investigate the interaction of osteogenesis and angiogenesis during bone healing. Combined techniques of Transwell co-culture, wound healing assay, enzyme linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qRT-PCR), western blotting and immunohistochemical staining were used to evaluate adhesion, morphology and migration of adipose-derived mesenchymal stem cells (Ad-MSCs) and human umbilical vein endothelial cells (HUVECs) grown on different Ti substrates...
July 1, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29701908/advances-in-osteobiologic-materials-for-bone-substitutes
#15
REVIEW
Anwarul Hasan, Batzaya Byambaa, Mahboob Morshed, Mohammad Ibrahim Cheikh, Rana Abdul Shakoor, Tanvir Mustafy, Hany Marei
A significant challenge in the current orthopedics is the development of suitable osteobiologic materials that can replace the conventional allografts, autografts, and xenografts and thereby serve as implant materials as bone substitutes for bone repair or remodelling. The complex biology behind the nanostructure and microstructure of bones and their repair mechanisms, which involve various types of chemical and biomechanical signalling amongst different cells, has set strong requirements for biomaterials to be used in bone tissue engineering...
April 27, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29696140/harnessing-nanotopography-to-enhance-osseointegration-of-clinical-orthopedic-titanium-implants-an-in-vitro-and-in-vivo-analysis
#16
Vitali Goriainov, Gry Hulsart-Billstrom, Terje Sjostrom, Douglas G Dunlop, Bo Su, Richard O C Oreffo
Despite technological advancements, further innovations in the field of orthopedics and bone regeneration are essential to meet the rising demands of an increasing aging population and associated issues of disease, injury and trauma. Nanotopography provides new opportunities for novel implant surface modifications and promises to deliver further improvements in implant performance. However, the technical complexities of nanotopography fabrication and surface analysis have precluded identification of the optimal surface features to trigger osteogenesis...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29679306/regenerative-medicine-strategies-in-biomedical-implants
#17
REVIEW
Divya Rani Bijukumar, Clay McGeehan, Mathew T Mathew
PURPOSE OF REVIEW: Recently, significant progress has been made in the research related to regenerative medicine. At the same time, biomedical implants in orthopedics and dentistry are facing many challenges and posing clinical concerns. The purpose of this chapter is to provide an overview of the clinical applications of current regenerative strategies to the fields of dentistry and orthopedic surgery. The main research question in this review is: What are the major advancement strategies in regenerative medicine that can be used for implant research? RECENT FINDINGS: The implant surfaces can be modified through patient-specific stem cells and plasma coatings, which may provide methods to improve osseointegration and sustainability of the implant...
June 2018: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/29673840/versatile-effects-of-magnesium-hydroxide-nanoparticles-in-plga-scaffold-mediated-chondrogenesis
#18
Kwang-Sook Park, Byoung-Ju Kim, Eugene Lih, Wooram Park, Soo-Hong Lee, Yoon Ki Joung, Dong Keun Han
Artificial scaffolds made up of various synthetic biodegradable polymers have been reported to have many advantages including cheap manufacturing, easy scale up, high mechanical strength, convenient manipulation, and molding into an unlimited variety of shapes. However, the synthetic biodegradable polymers still have the insufficiency for cartilage regeneration owing to their acidic degradation products. To reduce acidification by degradation of synthetic polymers, we incorporated magnesium hydroxide (MH) nanoparticles into porous polymer scaffold not only to effectively neutralize the acidic hydrolysate but also to minimize the structural disturbance of scaffolds...
June 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29666653/mesenchymal-stem-cells-in-oriented-plga-acecm-composite-scaffolds-enhance-structure-specific-regeneration-of-hyaline-cartilage-in-a-rabbit-model
#19
Weimin Guo, Xifu Zheng, Weiguo Zhang, Mingxue Chen, Zhenyong Wang, Chunxiang Hao, Jingxiang Huang, Zhiguo Yuan, Yu Zhang, Mingjie Wang, Jiang Peng, Aiyuan Wang, Yu Wang, Xiang Sui, Wenjing Xu, Shuyun Liu, Shibi Lu, Quanyi Guo
Articular cartilage lacks a blood supply and nerves. Hence, articular cartilage regeneration remains a major challenge in orthopedics. Decellularized extracellular matrix- (ECM-) based strategies have recently received particular attention. The structure of native cartilage exhibits complex zonal heterogeneity. Specifically, the development of a tissue-engineered scaffold mimicking the aligned structure of native cartilage would be of great utility in terms of cartilage regeneration. Previously, we fabricated oriented PLGA/ACECM (natural, nanofibrous, articular cartilage ECM) composite scaffolds...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29664925/actin-and-myosin-ii-modulate-differentiation-of-pluripotent-stem-cells
#20
Liana C Boraas, Emma T Pineda, Tabassum Ahsan
Use of stem cell-based therapies in tissue engineering and regenerative medicine is hindered by efficient means of directed differentiation. For pluripotent stem cells, an initial critical differentiation event is specification to one of three germ lineages: endoderm, mesoderm, and ectoderm. Differentiation is known to be regulated by numerous extracellular and intracellular factors, but the role of the cytoskeleton during specification, or early differentiation, is still unknown. In these studies, we used agonists and antagonists to modulate actin polymerization and the actin-myosin molecular motor during spontaneous differentiation of embryonic stem cells in embryoid bodies...
2018: PloS One
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