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Orthopedic stem cell

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https://www.readbyqxmd.com/read/29038672/safety-analysis-of-autologous-stem-cell-therapy-in-a-variety-of-degenerative-diseases-and-injuries-using-the-stromal-vascular-fraction
#1
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29033930/immunomodulatory-effects-of-calcium-and-strontium-co-doped-titanium-oxides-on-osteogenesis
#2
Xiangwei Yuan, Huiliang Cao, Jiaxing Wang, Kaiwei Tang, Bin Li, Yaochao Zhao, Mengqi Cheng, Hui Qin, Xuanyong Liu, Xianlong Zhang
The effects of calcium (Ca) or strontium (Sr) on host osteogenesis and immune responses have been investigated separately. In clinical practice, these two elements may both be present around an orthopedic device, but their potential synergistic effects on osteogenesis and the immune response have not been explored to date. In this work, we investigated the immunomodulatory effects of Ca and Sr co-doped titanium oxides on osteogenesis in vitro using the mouse macrophage cell line RAW 264.7 alone and in co-culture with mouse bone mesenchymal stem cells (BMSCs), and in vivo using a mouse air-pouch model...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28986619/-therapeutic-utilization-of-stem-cells-in-orthopedics
#3
C Chiari, S Walzer, D Stelzeneder, M Schreiner, R Windhager
Stem cells are becoming increasingly more important in the field of regenerative medicine. Adult mesenchymal stem cells (MSCs) are harvested predominantly from bone marrow or adipose tissue, are already being used in the clinical setting and have a low potential for side effects. In orthopedics, experience has been gained in the treatment of bone defects, non-unions, cartilage defects, osteoarthritis and tendon pathologies. The current data are derived from case studies and randomized controlled trials are missing; therefore, there are many open questions concerning the optimal cell source, number of cells, administration technique (e...
October 6, 2017: Der Orthopäde
https://www.readbyqxmd.com/read/28891421/bone-marrow-aspiration-for-regenerative-orthopedic-intervention-technique-with-ultrasound-guidance-for-needle-placement
#4
Shane A Shapiro, Jennifer R Arthurs
AIM: We review relevant anatomy of the iliac crest, and describe an interventional technique to maximize harvesting of desired progenitor cells with ultrasound to guide safe trochar placement. MATERIALS & METHODS: We validated the technique on both sides of the pelvis in four human cadavers. RESULTS: Using ultrasound guidance, 32 BMA needles were placed in a safe zone along various portions of the iliac crest. CONCLUSION: Ultrasound guidance can improve accuracy of bone marrow aspirations form the iliac crest...
September 11, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28879686/development-of-a-bioactive-porous-collagen-%C3%AE-tricalcium-phosphate-bone-graft-assisting-rapid-vascularization-for-bone-tissue-engineering-applications
#5
Nafiseh Baheiraei, Mohamma Reza Nourani, Sm Javad Mortazavi, Mansoureh Movahedin, Hossein Eyni, Fatemeh Baghery, Mohammad Hadi Norahan
We developed collagen (COL) and collagen/beta tricalcium phosphate (COL/β-TCP) scaffolds with a β-TCP/collagen weight ratio of 4 by freeze-drying. Mouse bone marrow-derived mesenchymal stem cells (BMMSCs) were cultured on these scaffolds for 14 days. Samples were characterized by physicochemical analyses and their biological properties such as cell viability and alkaline phosphatase (ALP) activity was,also, examined. Additionally, the vascularization potential of the prepared scaffolds was tested subcutaneously in Wistar rats...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28865450/elution-kinetics-of-vancomycin-and-gentamicin-from-carriers-and-their-effects-on-mesenchymal-stem-cell-proliferation-an-in-vitro-study
#6
Tomas Kucera, Lenka Ryskova, Tomas Soukup, Jana Malakova, Eva Cermakova, Pavel Mericka, Jakub Suchanek, Pavel Sponer
BACKGROUND: Musculoskeletal infections remain a major complication in orthopedic surgery. The local delivery of antibiotics provides the high levels required to treat an infection without systemic toxicity. However, the local toxicity of antibiotic carriers to the mesenchymal stem cells, as a result of both the peak concentrations and the type of carrier, may be significant. METHODS: To address this concern, the elution kinetics of vancomycin and gentamicin from several commercially available antibiotic carriers and several carriers impregnated by a surgeon (10 ml of each sterile carrier were manually mixed with a 500 mg vancomycin and an 80 mg gentamicin solution, and the duration of impregnation was 30 min) were assessed...
September 2, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28844671/transcriptional-profiling-of-human-femoral-mesenchymal-stem-cells-in-osteoporosis-and-its-association-with-adipogenesis
#7
Yong Jun Choi, Insun Song, Yilan Jin, Hyun-Seok Jin, Hyung Min Ji, Seon-Yong Jeong, Ye-Yeon Won, Yoon-Sok Chung
Genetic alterations are major contributing factors in the development of osteoporosis. Osteoblasts and adipocytes share a common origin, mesenchymal stem cells (MSCs), and their genetic determinants might be important in the relationship between osteoporosis and obesity. In the present study, we aimed to isolate differentially expressed genes (DEGs) in osteoporosis and normal controls using human MSCs, and elucidate the common pathways and genes related to osteoporosis and adipogenesis. Human MSCs were obtained from the bone marrow of femurs from postmenopausal women during orthopedic surgeries...
October 20, 2017: Gene
https://www.readbyqxmd.com/read/28818783/development-of-a-porous-3d-graphene-pdms-scaffold-for-improved-osseointegration
#8
Jianfeng Li, Xiao Liu, Jeremy M Crook, Gordon G Wallace
Osseointegration in orthopedic surgery plays an important role for bone implantation success. Traditional treatment of implant surface aimed at improved osseointegration has limited capability for its poor performance in supporting cell growth and proliferation. Polydimethylsiloxane (PDMS) is a widely used silicon-based organic polymer material with properties that are useful in cosmetics, domestic applications and mechanical engineering. In addition, the biocompatibility of PDMS, in part due to the high solubility of oxygen, makes it an ideal material for cell-based implants...
August 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28814956/rapid-rapamycin-only-induced-osteogenic-differentiation-of-blood-derived-stem-cells-and-their-adhesion-to-natural-and-artificial-scaffolds
#9
Carpentieri Arianna, Cozzoli Eliana, Acri Flavio, Ranalli Marco, Diedenhofen Giacomo, Scimeca Manuel, Bonanno Elena, Gambacurta Alessandra
Stem cells are a centerpiece of regenerative medicine research, and the recent development of adult stem cell-based therapy systems has vigorously expanded the scope and depth of this scientific field. The regeneration of damaged and/or degraded bone tissue in orthopedic, dental, or maxillofacial surgery is one of the main areas where stem cells and their regenerative potential could be used successfully, requiring tissue engineering solutions incorporating an ideal stem cell type paired with the correct mechanical support...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28811545/sealing-the-pores-of-peo-coating-with-mg-al-layered-double-hydroxide-enhanced-corrosion-resistance-cytocompatibility-and-drug-delivery-ability
#10
Feng Peng, Donghui Wang, Yaxin Tian, Huiliang Cao, Yuqin Qiao, Xuanyong Liu
In recent years, magnesium (Mg) alloys show a promising application in clinic as degradable biomaterials. Nevertheless, the poor corrosion resistance of Mg alloys is the main obstacle to their clinical application. Here we successfully seal the pores of plasma electrolytic oxidation (PEO) coating on AZ31 with Mg-Al layered double hydroxide (LDH) via hydrothermal treatment. PEO/LDH composite coating possess a two layer structure, an inner layer made up of PEO coating (~5 μm) and an outer layer of Mg-Al LDH (~2 μm)...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28766102/a-new-chapter-for-mesenchymal-stem-cells-decellularized-extracellular-matrices
#11
REVIEW
Yagiz Anasiz, Riza Koksal Ozgul, Duygu Uckan-Cetinkaya
From orthopedic to neurological disorders, stem cells are used as platforms to understand disease mechanisms and considered as novel and promising treatment options, especially when the valid therapeutic approaches are unavailable or ineffective. There are different stem cell types in the literature, however the spindle-shaped, colony forming and multilineage-differentiating cells, also known as mesenchymal stem cells (MSC) are very popular, as MSC can be isolated from different tissues with minimal ethical concerns and without tumor formations, which make them easily accessible and widely used in vitro and in vivo studies...
August 1, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28747099/in-vivo-models-for-the-evaluation-of-the-osteogenic-potency-of-bone-substitutes-seeded-with-mesenchymal-stem-cells-of-human-origin-a-concise-review
#12
Fabian Westhauser, Anne-Sophie Senger, Bruno Reible, Arash Moghaddam
Research concerning bone substitutes is one of the most challenging fields in orthopedic research and has a high clinical relevance, especially since the currently available bone substitutes are limited in their osteostimulative capabilities. In vitro models for the evaluation of the properties of bone substitutes allow the use of human mesenchymal stem cells (hMSCs) seeded onto scaffolds, but suffer from the lack of a physiological environment for those cells. Most in vivo models include the use of non-hMSC and are therefore lacking in clinical relevance...
September 1, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28698718/the-holy-grail-of-orthopedic-surgery-mesenchymal-stem-cells-their-current-uses-and-potential-applications
#13
REVIEW
Roberto Berebichez-Fridman, Ricardo Gómez-García, Julio Granados-Montiel, Enrique Berebichez-Fastlicht, Anell Olivos-Meza, Julio Granados, Cristina Velasquillo, Clemente Ibarra
Only select tissues and organs are able to spontaneously regenerate after disease or trauma, and this regenerative capacity diminishes over time. Human stem cell research explores therapeutic regenerative approaches to treat various conditions. Mesenchymal stem cells (MSCs) are derived from adult stem cells; they are multipotent and exert anti-inflammatory and immunomodulatory effects. They can differentiate into multiple cell types of the mesenchyme, for example, endothelial cells, osteoblasts, chondrocytes, fibroblasts, tenocytes, vascular smooth muscle cells, and sarcomere muscular cells...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28672942/adenovirus-mediated-bone-morphogenetic-protein-2-promotes-osteogenic-differentiation-in-human-mesenchymal-stem-cells-in-vitro
#14
Hong Cao, Zhi-Bo Sun, Lei Zhang, Wei Qian, Chun-Yang Li, Xiao-Peng Guo, Ying Zhang
Delayed and failed bone union following fracture is a common clinical complication that requires treatment in orthopedics. Cell-based therapies and tissue-engineering approaches are potential therapeutic strategies for bone repair and fracture healing. However, the effect of adenovirus expressing bone morphogenetic protein-2 (Ad-BMP-2) on the osteogenic ability of human mesenchymal stem cells (hMSCs) has remained to be fully elucidated. Therefore, in the present study, hMSCs were transduced using Ad-BMP-2 to assess the effects of its application and to determine whether Ad-BMP-2 promotes the osteogenic differentiation of hMSCs...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28664842/difference-between-biomarkers-of-tibial-bone-marrow-and-adipose-tissue
#15
Ersin Kuyucu, Mehmet Erdil, Adnan Kara, Murat Bülbül
BACKGROUND: Stem cells, with their regeneration capacity, long-term viability, and differentiation characteristics, have indispensable biological properties. As described by Hauner and Grigoradis et al., mesenchymal stem cell originating from adipose or bone marrow can be differentiated into many tissues such as adipocyte, chondrocyte, myeloblast, and osteoblast. The aim of our study is to compare the use of adipose and tibial bone marrow derived stem cells for therapeutic purposes in orthopedic surgery, which has not been clearly evaluated in the literature to our knowledge and to also evaluate their use...
2017: SICOT-J
https://www.readbyqxmd.com/read/28639702/bone-marrow-aspirate-clot-a-technical-complication-or-a-smart-approach-for-musculoskeletal-tissue-regeneration
#16
REVIEW
Francesca Salamanna, Deyanira Contartese, Nicolò Nicoli Aldini, Giovanni Barbanti Brodano, Cristiana Griffoni, Alessandro Gasbarrini, Milena Fini
One of the methods employed to improve healing of damaged tissues is the use of cellular based therapies. A number of regenerative medicine based strategies, from in vitro expanded mesenchymal stem cells (MSCs) to "one-step" procedures using bone marrow (BM) in toto (BM aspirate; BMA) or BM concentrate (BMC), have been developed. Recently, orthopedic researchers focused their attention on the clinical therapeutic potential of BMC and BMA for musculoskeletal regeneration. BMA is reported as an excellent source of cells and growth factors...
June 22, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28635177/engineering-human-bone-grafts-with-new-macroporous-calcium-phosphate-cement-scaffolds
#17
Martina Sladkova, Michael Palmer, Caroline Öhman, Jiayi Cheng, Shoug Al-Ansari, Munerah Saad, Håkan Engqvist, Giuseppe Maria de Peppo
Bone engineering opens the possibility to grow large amounts of tissue products by combining patient-specific cells with compliant biomaterials. Decellularized tissue matrices represent suitable biomaterials but availability, long processing time, excessive cost, and concerns on pathogen transmission have led to the development of biomimetic synthetic alternatives. We recently fabricated calcium phosphate cement (CPC) scaffolds with variable macroporosity using a facile synthesis method with minimal manufacturing steps, and demonstrated long-term biocompatibility in vitro...
June 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28611002/chondrogenesis-of-human-bone-marrow-mesenchymal-stem-cells-in-3-dimensional-photocrosslinked-hydrogel-constructs-effect-of-cell-seeding-density-and-material-stiffness
#18
Aaron X Sun, Hang Lin, Madalyn R Fritch, He Shen, Pete G Alexander, Michael DeHart, Rocky S Tuan
Three-dimensional hydrogel constructs incorporated with live stem cells that support chondrogenic differentiation and maintenance offer a promising regenerative route towards addressing the limited self-repair capabilities of articular cartilage. In particular, hydrogel scaffolds that augment chondrogenesis and recapitulate the native physical properties of cartilage, such as compressive strength, can potentially be applied in point-of-care procedures. We report here the synthesis of two new materials, [poly-l-lactic acid/polyethylene glycol/poly-l-lactic acid] (PLLA-PEG 1000) and [poly-d,l-lactic acid/polyethylene glycol/poly-d,l-lactic acid] (PDLLA-PEG 1000), that are biodegradable, biocompatible (>80% viability post fabrication), and possess high, physiologically relevant mechanical strength (∼1500 to 1800kPa)...
June 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28585995/outcomes-of-primary-total-knee-arthroplasty-in-patients-with-hematopoietic-stem-cell-transplantation
#19
Brian P Chalmers, Cameron K Ledford, Kevin I Perry, Tad M Mabry, Arlen D Hanssen, Matthew P Abdel
Patients who have undergone hematopoietic stem cell transplantation to treat underlying bone marrow pathology represent a unique and potentially high-risk patient population for total knee arthroplasty (TKA). This study retrospectively reviewed 15 TKA procedures performed on 11 patients with a history of hematopoietic stem cell transplantation. The authors analyzed patient survivorship; clinical outcomes, including complications; and implant survivorship. Mean follow-up was 5 years (range, 2-10 years). Patient survivorship free from mortality was 91% (95% confidence interval, 76%-100%) and 55% (95% confidence interval, 25%-85%) at 2 and 5 years, respectively...
September 1, 2017: Orthopedics
https://www.readbyqxmd.com/read/28577780/role-of-bone-marrow-aspirate-in-orthopedic-trauma
#20
REVIEW
Patrick C Schottel, Stephen J Warner
Bone marrow aspirate grafting entails mesenchymal stem cell-containing bone marrow harvesting and injection into a fracture site to promote bone formation. Although the use of bone marrow aspirate in orthopedic trauma is not widespread, an increasing number of studies are reporting clinical success. Advantages of using bone marrow aspirate are that it is readily obtainable, has low harvest morbidity, and can be easily and quickly injected. However, no universally accepted role for its use exists. Future studies directly comparing bone marrow aspirate with conventional techniques are needed to define its role in the treatment of orthopedic trauma patients...
July 2017: Orthopedic Clinics of North America
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