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Hip osteoarthritis stem cell

Jose M Vilar, Belen Cuervo, Monica Rubio, Joaquín Sopena, Juan M Domínguez, Angelo Santana, Jose M Carrillo
BACKGROUND: Subjective pain assessment scales have been widely used for assessing lameness in response to pain, but the accuracy of these scales has been questioned. To assess scale accuracy, 10 lame, presa Canario dogs with osteoarthritis (OA) associated with bilateral hip dysplasia were first treated with mesenchymal stem cells. Then, potential lameness improvement was analyzed using two pain scales (Bioarth and visual analog scale). These data were compared with similar data collected using a force platform with the same animals during a period of 6 months after treatment...
October 7, 2016: BMC Veterinary Research
Ahmed M Samy
BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a debilitating disease in orthopedics, frequently progressing to femoral head collapse and osteoarthritis. It is thought to be a multifactorial disease. ONFH ultimately results in femoral head collapse in 75-85% of untreated patients. Total hip arthroplasty (THA) yields satisfactory results in the treatment of the end stage of the disease. However, disease typically affects males between the ages of 20 and 40 years and joint replacement is not the ideal option for younger patients...
July 2016: Indian Journal of Orthopaedics
Jorge Chahla, Robert F LaPrade, Rodrigo Mardones, Johnny Huard, Marc J Philippon, Shane Nho, Omer Mei-Dan, Cecilia Pascual-Garrido
Treatment of hip cartilage disease is challenging, and there is no clear algorithm to address this entity. Biomarkers are arising as promising diagnostic tools because they could play a role in the early assessment of the prearthritic joint and as a prognostic factor before and after treatment. The potential effect of biomarkers may be used to categorize individuals at risk of evolving to severe osteoarthritis, to develop new measures for clinical progression of the disease, and to develop new treatment options for the prevention of osteoarthritis progression...
July 1, 2016: Orthopedics
Maik Stiehler, Juliane Rauh, Cody Bünger, Angela Jacobi, Corina Vater, Theresa Schildberg, Cornelia Liebers, Klaus-Peter Günther, Henriette Bretschneider
BMSCs, also known as bone marrow-derived mesenchymal stem cells, provide an excellent source of progenitor cells for regenerative therapy. To assess whether osteoarthritis (OA) affects the regenerative potential of BMSCs we compared the proliferation and differentiation potential as well as the surface marker expression profile of OA- versus control BMSCs. BMSCs were isolated from bone marrow aspirates of n=14 patients with advanced-stage idiopathic hip OA (67±6years) and n=15 healthy individuals (61±4years)...
May 2016: Stem Cell Research
Subhash C Juneja, Sowmya Viswanathan, Milan Ganguly, Christian Veillette
The procedure for aspiration of bone marrow from the femur of patients undergoing total knee arthroplasty (TKA) or total hip arthroplasty (THA) may vary from an OR (operating room) to OR based on the surgeon's skill and may lead to varied extent of clotting of the marrow and this, in turn, presents difficulty in the isolation of mesenchymal stem cells (MSCs) from such clotted bone marrow. We present a simple detailed protocol for aspirating bone marrow from such patients, isolation, and characterization of MSCs from the aspirated bone marrow specimens and show that the bone marrow presented no clotting or exhibited minimal clotting...
2016: Bone Marrow Research
Jorge Cabrolier, Marcelo Molina
Osteonecrosis of the femoral head leads to degeneration of the head and finally to osteoarthritis of the hip. Decompression is the most widely used treatment, but its effectiveness is limited. It has been proposed instillation of stem cells in addition to decompression, would lead to better results. Searching in Epistemonikos database, which is maintained by screening 30 databases, we identified two systematic reviews including two randomized trials. We combined the evidence using meta-analysis and generated a summary of findings table following the GRADE approach...
2016: Medwave
José M Vilar, Mónica Rubio, Giuseppe Spinella, Belén Cuervo, Joaquín Sopena, Ramón Cugat, Montserrat Garcia-Balletbó, Juan M Dominguez, Maria Granados, Asta Tvarijonaviciute, José J Ceron, José M Carrillo
The aim of this study was to evaluate the use of serum type II collagen cleavage epitope and serum hyaluronic acid as biomarkers for treatment monitoring in osteoarthritic dogs. For this purpose, a treatment model based on mesenchymal stem cells derived from adipose tissue combined with plasma rich in growth factors was used. This clinical study included 10 dogs with hip osteoarthritis. Both analytes were measured in serum at baseline, just before applying the treatment, and 1, 3, and 6 months after treatment...
2016: PloS One
T Mark Campbell, Sarah M Churchman, Alejandro Gomez, Dennis McGonagle, Philip G Conaghan, Frederique Ponchel, Elena Jones
OBJECTIVE: In patients with osteoarthritis (OA), bone marrow lesions (BMLs) are intimately linked to disease progression. We hypothesized that aberrant multipotential stromal cell (also known as mesenchymal stem cell [MSC]) responses within bone tissue contributes to BML pathophysiology. The aim of this study was to investigate BML and non-BML native subchondral bone MSCs for numeric, topographic, in vitro functional, and gene expression differences. METHODS: Ex vivo 3T magnetic resonance imaging (MRI) of the femoral heads of 20 patients with hip OA was performed...
July 2016: Arthritis & Rheumatology
Masaaki Sakamoto, Hitoshi Watanabe, Hidetaka Higashi, Hitoshi Kubosawa
A 77-year-old woman underwent metal-on-polyethylene total hip arthroplasty for osteoarthritis of the right hip at another institution. During surgery, the greater trochanter was broken and internal fixation was performed with a trochanteric cable grip reattachment. Although postoperative recovery was uneventful, approximately 6 years later, the patient had severe right hip pain with apparent swelling, and she was referred to the authors' institution. Plain radiographs showed evidence of severe osteolysis in the proximal femur and cable breakage; however, preoperative aspiration culture findings were negative for bacterial growth...
January 2016: Orthopedics
S J B Snelling, R K Davidson, T E Swingler, L T T Le, M J Barter, K L Culley, A Price, A J Carr, I M Clark
OBJECTIVE: Dickkopf-3 (Dkk3) is a non-canonical member of the Dkk family of Wnt antagonists and its upregulation has been reported in microarray analysis of cartilage from mouse models of osteoarthritis (OA). In this study we assessed Dkk3 expression in human OA cartilage to ascertain its potential role in chondrocyte signaling and cartilage maintenance. METHODS: Dkk3 expression was analysed in human adult OA cartilage and synovial tissues and during chondrogenesis of ATDC5 and human mesenchymal stem cells...
May 2016: Osteoarthritis and Cartilage
Katherine Johnson, Louise N Reynard, John Loughlin
BACKGROUND: The arcOGEN genome-wide association study reported the rs9350591 C/T single nucleotide polymorphism (SNP) as marking a region on chromosome 6q14.1 that is associated with hip osteoarthritis (OA) in Europeans, with an odds ratio (OR) of 1.18 and a p-value of 2.42 × 10(-9). rs9350591 is an intergenic SNP surrounded by seven genes within 1 Mb. Six of the genes are expressed in cartilage. We sought to characterise this signal to assess whether the association of rs9350591 with OA is mediated by modulating gene expression...
2015: BMC Medical Genetics
Reza Mostafavi Tabatabaee, Sadegh Saberi, Javad Parvizi, Seyed Mohammad Javad Mortazavi, Mahmoud Farzan
The management of early-stage osteonecrosis of the femoral head (ONFH) remains challenging. This study aimed to evaluate the effects of core decompression and concentrated bone marrow implantation on ONFH. The study recruited 28 hips with early ONFH randomly assigned into two groups of core decompression with (group A) and without (group B) bone marrow injection. Patients were evaluated using the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) questionnaire, Visual Analogue Scale (VAS) pain index, and MRI...
September 2015: Journal of Arthroplasty
Mohsen Emadedin, Maede Ghorbani Liastani, Roghayeh Fazeli, Fatemeh Mohseni, Reza Moghadasali, Soura Mardpour, Seyyedeh Esmat Hosseini, Maryam Niknejadi, Fatemeh Moeininia, Aslan Aghahossein Fanni, Reza Baghban Eslaminejhad, Ahmad Vosough Dizaji, Narges Labibzadeh, Ali Mirazimi Bafghi, Hossein Baharvand, Nasser Aghdami
BACKGROUND: Osteoarthritis (OA) is a debilitating disease that typically affects a large number of the middle-aged and elderly population. Current treatment strategies have had limited success in these patients. This study aims to investigate the safety of treatment with autologous bone marrow (BM)-derived mesenchymal stem cells (MSCs) transplanted in patients with OA of the knee, ankle, or hip. METHODS: We enrolled 18 patients with different joint involvements (knee, ankle, or hip OA) and one was lost to follow-up...
June 2015: Archives of Iranian Medicine
Andre F Steinert, Manuela Kunz, Patrick Prager, Sascha Göbel, Ludger Klein-Hitpass, Regina Ebert, Ulrich Nöth, Franz Jakob, Frank Gohlke
INTRODUCTION: The bursa subacromialis (BS) provides the gliding mechanism of the shoulder and regenerates itself after surgical removal. Therefore, we explored the presence of mesenchymal stem cells (MSCs) within the human adult BS tissue and characterized the BS cells compared to MSCs from bone marrow (BMSCs) on a molecular level. METHODS: BS cells were isolated by collagenase digest from BS tissues derived from patients with degenerative rotator cuff tears, and BMSCs were recovered by adherent culture from bone-marrow of patients with osteoarthritis of the hip...
2015: Stem Cell Research & Therapy
Jaroslav Michalek, Rene Moster, Ladislav Lukac, Kenneth Proefrock, Miron Petrasovic, Jakub Rybar, Martina Capkova, Ales Chaloupka, Adas Darinskas, Jaroslav Michalek, Jan Kristek, Jan Travnik, Petr Jabandziev, Marek Cibulka, Michal Holek, Michal Jurik, Josef Skopalik, Zlatuse Kristkova, Zuzana Dudasova
Stromal vascular fraction (SVF), containing large amount of stem cells and other regenerative cells, can be easily obtained from loose connective tissue that is associated with adipose tissue. Here we evaluated safety and clinical efficacy of freshly isolated autologous SVF cells in a case control study in patients with grade 2-4 degenerative osteoarthritis (OA). A total of 1128 patients underwent standard liposuction under local anesthesia and SVF cells were isolated and prepared for application into 1-4 large joints...
January 20, 2015: Cell Transplantation
Shunji Tomatsu, Carlos J Alméciga-Díaz, Adriana M Montaño, Hiromasa Yabe, Akemi Tanaka, Vu Chi Dung, Roberto Giugliani, Francyne Kubaski, Robert W Mason, Eriko Yasuda, Kazuki Sawamoto, William Mackenzie, Yasuyuki Suzuki, Kenji E Orii, Luis A Barrera, William S Sly, Tadao Orii
Patients with mucopolysaccharidoses (MPS) have accumulation of glycosaminoglycans in multiple tissues which may cause coarse facial features, mental retardation, recurrent ear and nose infections, inguinal and umbilical hernias, hepatosplenomegaly, and skeletal deformities. Clinical features related to bone lesions may include marked short stature, cervical stenosis, pectus carinatum, small lungs, joint rigidity (but laxity for MPS IV), kyphoscoliosis, lumbar gibbus, and genu valgum. Patients with MPS are often wheelchair-bound and physical handicaps increase with age as a result of progressive skeletal dysplasia, abnormal joint mobility, and osteoarthritis, leading to 1) stenosis of the upper cervical region, 2) restrictive small lung, 3) hip dysplasia, 4) restriction of joint movement, and 5) surgical complications...
February 2015: Molecular Genetics and Metabolism
Emilio Camafeita, José-Ramón Lamas, Enrique Calvo, Pilar Tornero-Esteban, Juan-Antonio López, Benjamín Fernández-Gutiérrez
Osteoarthritis (OA) is considered the most prevalent form of arthritis. The aim of this study was to verify potential protein OA biomarkers by applying Selected Reaction Monitoring (SRM) assays to protein extracts obtained from Bone Marrow-Mesenchymal Stem Cells (BM-MSCs) isolated from OA patients. BM aspirates were obtained from the femoral channel of OA patients at the time of surgery and from the femoral channel of hip fracture subjects without OA during hip joint replacement surgery for the treatment of subcapital fracture...
2014: Clinical Proteomics
R F P Schelbergen, W de Munter, M H J van den Bosch, F P J G Lafeber, A Sloetjes, T Vogl, J Roth, W B van den Berg, P M van der Kraan, A B Blom, P L E M van Lent
OBJECTIVE: Alarmins S100A8 and S100A9 are major products of activated macrophages regulating cartilage damage and synovial activation during murine and human osteoarthritis (OA). In the current study, we investigated whether S100A8 and S100A9 are involved in osteophyte formation during experimental OA and whether S100A8/A9 predicts osteophyte progression in early human OA. METHODS: OA was elicited in S100A9-/- mice in two experimental models that differ in degree of synovial activation...
January 2016: Annals of the Rheumatic Diseases
Abdulrahman M Alaseem, Padma Madiraju, Sultan A Aldebeyan, Hussain Noorwali, John Antoniou, Fackson Mwale
Several studies have shown that type X collagen (COL X), a marker of late-stage chondrocyte hypertrophy, is expressed in mesenchymal stem cells (MSCs) from osteoarthritis (OA) patients. We recently found that Naproxen, but not other nonsteroidal anti-inflammatory drugs (NSAIDs) (Ibuprofen, Celebrex, Diclofenac), can induce type X collagen gene (COL10A1) expression in bone-marrow-derived MSCs from healthy and OA donors. In this study we determined the effect of Naproxen on COL X protein expression and investigated the intracellular signaling pathways that mediate Naproxen-induced COL10A1 expression in normal and OA hMSCs...
January 2015: Tissue Engineering. Part A
Belen Cuervo, Monica Rubio, Joaquin Sopena, Juan Manuel Dominguez, Jose Vilar, Manuel Morales, Ramón Cugat, Jose Maria Carrillo
PURPOSE: The aim of this study was to compare the efficacy and safety of a single intra-articular injection of adipose mesenchymal stem cells (aMSCs) versus plasma rich in growth factors (PRGF) as a treatment for reducing symptoms in dogs with hip osteoarthritis (OA). METHODS: This was a randomized, multicenter, blinded, parallel group. Thirty-nine dogs with symptomatic hip OA were assigned to one of the two groups, to receive aMSCs or PRGF. The primary outcome measures were pain and function subscales, including radiologic assessment, functional limitation and joint mobility...
2014: International Journal of Molecular Sciences
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