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Rotator cuff stem cell

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https://www.readbyqxmd.com/read/27887870/involvement-of-indian-hedgehog-signaling-in-mesenchymal-stem-cell-augmented-rotator-cuff-tendon-repair-in-an-athymic-rat-model
#1
Jian-Chun Zong, Michael J Mosca, Ryan M Degen, Amir Lebaschi, Camila Carballo, Andrew Carbone, Guang-Ting Cong, Liang Ying, Xiang-Hua Deng, Scott A Rodeo
BACKGROUND: Bone marrow aspirate has been used in recent years to augment tendon-to-bone healing, including in rotator cuff repair. However, the healing mechanism in cell-based therapy has not been elucidated in detail. METHODS: Sixteen athymic nude rats were randomly allocated to 2 groups: experimental (human mesenchymal stem cells in fibrin glue carrier) and control (fibrin glue only). Animals were sacrificed at 2 and 4 weeks. Immunohistochemical staining was performed to evaluate Indian hedgehog (Ihh) signaling and SOX9 signaling in the healing enthesis...
November 22, 2016: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/27768610/biologic-and-synthetic-grafts-in-the-reconstruction-of-large-to-massive-rotator-cuff-tears
#2
Robert J Gillespie, Derrick M Knapik, Ozan Akkus
Rotator cuff injuries are common in both young and elderly patients. Despite improvements in instrumentation and surgical techniques, the failure rates following tendon reconstruction remain unacceptably high. To improve outcomes, graft patches have been developed to provide mechanical strength and to furnish a scaffold for biologic growth across the delicate tendon-bone junction. Although no patch effectively re-creates the structured, highly organized system of prenatal tendon development, augmenting rotator cuff repair may help restore native tendon-to-bone attachment while reproducing the mechanical and biologic properties of native tendon...
December 2016: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/27750374/advances-in-biologic-augmentation-for-rotator-cuff-repair
#3
Sahishnu Patel, Anthony P Gualtieri, Helen H Lu, William N Levine
Rotator cuff tear is a very common shoulder injury that often necessitates surgical intervention for repair. Despite advances in surgical techniques for rotator cuff repair, there is a high incidence of failure after surgery because of poor healing capacity attributed to many factors. The complexity of tendon-to-bone integration inherently presents a challenge for repair because of a large biomechanical mismatch between the tendon and bone and insufficient regeneration of native tissue, leading to the formation of fibrovascular scar tissue...
November 2016: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/27708732/the-role-of-platelet-rich-plasma-prp-and-other-biologics-for-rotator-cuff-repair
#4
Joshua A Greenspoon, Samuel G Moulton, Peter J Millett, Maximilian Petri
BACKGROUND: Surgical treatment of rotator cuff tears has consistently demonstrated good clinical and functional outcomes. However, in some cases, the rotator cuff fails to heal. While improvements in rotator cuff constructs and biomechanics have been made, the role of biologics to aid healing is currently being investigated. METHODS: A selective literature search was performed and personal surgical experiences are reported. RESULTS: Biologic augmentation of rotator cuff repairs can for example be performed wtableith platelet-rich plasma (PRP) and mesenchymal stem cells (MSCs)...
2016: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/27606597/tendon-reattachment-to-bone-in-an-ovine-tendon-defect-model-of-retraction-using-allogenic-and-xenogenic-demineralised-bone-matrix-incorporated-with-mesenchymal-stem-cells
#5
Tanujan Thangarajah, Shirin Shahbazi, Catherine J Pendegrass, Simon Lambert, Susan Alexander, Gordon W Blunn
BACKGROUND: Tendon-bone healing following rotator cuff repairs is mainly impaired by poor tissue quality. Demineralised bone matrix promotes healing of the tendon-bone interface but its role in the treatment of tendon tears with retraction has not been investigated. We hypothesized that cortical demineralised bone matrix used with minimally manipulated mesenchymal stem cells will result in improved function and restoration of the tendon-bone interface with no difference between xenogenic and allogenic scaffolds...
2016: PloS One
https://www.readbyqxmd.com/read/27538432/effects-of-the-pulsed-electromagnetic-field-pst%C3%A2-on-human-tendon-stem-cells-a-controlled-laboratory-study
#6
Pietro Randelli, Alessandra Menon, Vincenza Ragone, Pasquale Creo, Umberto Alfieri Montrasio, Carlotta Perucca Orfei, Giuseppe Banfi, Paolo Cabitza, Guido Tettamanti, Luigi Anastasia
BACKGROUND: Current clinical procedures for rotator cuff tears need to be improved, as a high rate of failure is still observed. Therefore, new approaches have been attempted to stimulate self-regeneration, including biophysical stimulation modalities, such as low-frequency pulsed electromagnetic fields, which are alternative and non-invasive methods that seem to produce satisfying therapeutic effects. While little is known about their mechanism of action, it has been speculated that they may act on resident stem cells...
August 18, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27501832/mesenchymal-stem-cell-secretome-a-potential-tool-for-the-prevention-of-muscle-degenerative-changes-associated-with-chronic-rotator-cuff-tears
#7
Nuno Sevivas, Fábio Gabriel Teixeira, Raquel Portugal, Luís Araújo, Luís Filipe Carriço, Nuno Ferreira, Manuel Vieira da Silva, João Espregueira-Mendes, Sandra Anjo, Bruno Manadas, Nuno Sousa, António J Salgado
BACKGROUND: Massive rotator cuff tears (MRCTs) are usually chronic lesions with pronounced degenerative changes, where advanced fatty degeneration and atrophy can make the tear irreparable. Human mesenchymal stem cells (hMSCs) secrete a range of growth factors and vesicular systems, known as secretome, that mediates regenerative processes in tissues undergoing degeneration. PURPOSE: To study the effect of hMSC secretome on muscular degenerative changes and shoulder function on a rat MRCT model...
August 8, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27353434/extracellular-matrix-of-current-biological-scaffolds-promotes-the-differentiation-potential-of-mesenchymal-stem-cells
#8
Andreas Voss, Mary Beth McCarthy, Alexander Hoberman, Mark P Cote, Andreas B Imhoff, Augustus D Mazzocca, Knut Beitzel
PURPOSE: The purpose of this study was to quantitatively assess the ability of bone marrow-derived mesenchymal stem cells (bMSC) to differentiate toward bone, fat, cartilage, and tendon lineages when grown on commercially available scaffolds compared with control and native tendon tissue. METHODS: BMSCs were cultured and analyzed by fluorescent automated cells sorting for surface markers CD73, -90, and -105. BMSCs were grown on rotator cuff tendon (RCT), decellularized human dermis patch (DDP), bilayer collagen matrix, and fibrin matrix (FM) to test their differentiation potential using quantitative polymerase chain reaction and establish markers for osteogenic, adipogenic, chondrogenic, and tenogenic lineages...
June 25, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/27282111/the-effect-of-purified-human-bone-marrow-derived-mesenchymal-stem-cells-on-rotator-cuff-tendon-healing-in-an-athymic-rat
#9
Ryan M Degen, Andrew Carbone, Camila Carballo, Jianchun Zong, Tony Chen, Amir Lebaschi, Liang Ying, Xiang-Hua Deng, Scott A Rodeo
PURPOSE: To evaluate the ability of purified human bone marrow-derived mesenchymal stem cells (MSCs) to augment healing of an acute small- to medium-sized rotator cuff repair in a small-animal model, evaluating the structure and composition of the healing tendon-bone interface with histologic and biomechanical analyses. METHODS: Fifty-two athymic rats underwent unilateral detachment and transosseous repair of the supraspinatus tendon augmented with either fibrin glue (control group) or fibrin glue with 10(6) human MSCs (experimental group) applied at the repair site...
June 7, 2016: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/27057170/lipogems-product-treatment-increases-the-proliferation-rate-of-human-tendon-stem-cells-without-affecting-their-stemness-and-differentiation-capability
#10
Pietro Randelli, Alessandra Menon, Vincenza Ragone, Pasquale Creo, Sonia Bergante, Filippo Randelli, Laura De Girolamo, Umberto Alfieri Montrasio, Giuseppe Banfi, Paolo Cabitza, Guido Tettamanti, Luigi Anastasia
Increasing the success rate of rotator cuff healing remains tremendous challenge. Among many approaches, the possibility of activating resident stem cells in situ, without the need to isolate them from biopsies, could represent valuable therapeutic strategy. Along this line, it has been recently demonstrated that lipoaspirate product, Lipogems, contains and produces growth-factors that may activate resident stem cells. In this study, human tendon stem cells (hTSCs) from the rotator cuff were cocultured in a transwell system with the Lipogems lipoaspirate product and compared to control untreated cells in terms of cell proliferation, morphology, stem cell marker and VEGF expression, and differentiation and migration capabilities...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27036202/characterization-of-progenitor-cells-derived-from-torn-human-rotator-cuff-tendons-by-gene-expression-patterns-of-chondrogenesis-osteogenesis-and-adipogenesis
#11
Issei Nagura, Takeshi Kokubu, Yutaka Mifune, Atsuyuki Inui, Fumiaki Takase, Yasuhiro Ueda, Takeshi Kataoka, Masahiro Kurosaka
BACKGROUND: It is important to regenerate the tendon-to-bone interface after rotator cuff repair to prevent re-tears. The cells from torn human rotator cuff were targeted, and their capacity for multilineage differentiation was investigated. METHODS: The edges of the rotator cuff were harvested during arthroscopic rotator cuff repair from nine patients, minced into pieces, and cultured on dishes. Adherent cells were cultured, phenotypically characterized. Then expandability, differentiation potential and gene expression were analyzed...
March 31, 2016: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/26824451/tob1-deficiency-enhances-the-effect-of-bone-marrow-derived-mesenchymal-stem-cells-on-tendon-bone-healing-in-a-rat-rotator-cuff-repair-model
#12
Yulei Gao, Yinquan Zhang, Yanghu Lu, Yi Wang, Xingrui Kou, Yi Lou, Yifan Kang
BACKGROUND/AIMS: This study investigated the effect of silencing TOB1 (Transducer of ERBB2, 1) expression in bone marrow-derived mesenchymal stem cells (MSCs) on MSC-facilitated tendon-bone healing in a rat supraspinatus repair model. METHODS: Rat MSCs were transduced with a recombinant lentivirus encoding short hairpin RNA (shRNA) against TOB1. MSC cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. The effect of MSCs with TOB1 deficiency on tendon-bone healing in a rat rotator cuff repair model was evaluated by biomechanical testing, histological analysis and collagen type I and II gene expression...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/26371340/regeneration-of-full-thickness-rotator-cuff-tendon-tear-after-ultrasound-guided-injection-with-umbilical-cord-blood-derived-mesenchymal-stem-cells-in-a-rabbit-model
#13
Gi-Young Park, Dong Rak Kwon, Sang Chul Lee
UNLABELLED: Rotator cuff tendon tear is one of the most common causes of chronic shoulder pain and disability. In this study, we investigated the therapeutic effects of ultrasound-guided human umbilical cord blood (UCB)-derived mesenchymal stem cell (MSC) injection to regenerate a full-thickness subscapularis tendon tear in a rabbit model by evaluating the gross morphology and histology of the injected tendon and motion analysis of the rabbit's activity. At 4 weeks after ultrasound-guided UCB-derived MSC injection, 7 of the 10 full-thickness subscapularis tendon tears were only partial-thickness tears, and 3 remained full-thickness tendon tears...
November 2015: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/26317295/-use-of-cultured-human-autologous-bone-marrow-stem-cells-in-repair-of-a-rotator-cuff-tear-preliminary-results-of-a-safety-study
#14
V Havlas, J Kotaška, P Koníček, T Trč, Š Konrádová, Z Kočí, E Syková
PURPOSE OF THE STUDY Rotator cuff tears are one of the most frequent shoulder disorders which are often associated with pain and interfere with proper arm function. In order to evaluate the safety and effectiveness of using cultured human autologous mesenchymal stem cells (MSC) applied to the suture site during arthroscopic repair of a rotator cuff tear, a prospective clinical study was designed and started recently at the authors' department. Its primary goal was to evaluate the safety of using cultured human MSCs, the secondary goal then was to study a therapeutic effect of their application...
2015: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/26311443/fgf-2-stimulates-the-growth-of-tenogenic-progenitor-cells-to-facilitate-the-generation-of-tenomodulin-positive-tenocytes-in-a-rat-rotator-cuff-healing-model
#15
Takuya Tokunaga, Chisa Shukunami, Nobukazu Okamoto, Takuya Taniwaki, Kiyoshi Oka, Hidetoshi Sakamoto, Junji Ide, Hiroshi Mizuta, Yuji Hiraki
BACKGROUND: Fibroblast growth factor (FGF)-2 has the potential to enhance tendon-to-bone healing after rotator cuff (RC) injury. HYPOTHESIS: FGF-2 stimulates tenogenic differentiation of progenitors to improve the biomechanical strength and histological appearance of repaired RCs in rats. STUDY DESIGN: Controlled laboratory study. METHODS: Adult male Sprague-Dawley rats (N = 156) underwent unilateral surgery to repair the supraspinatus tendon to insertion sites...
October 2015: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/26311024/-stem-cells-in-orthopaedics
#16
REVIEW
Pavel Neckař, Eva Syková
Sharing new ideas and approaches is needed to advance basic scientific research as well as the clinical application of stem cells. In this newsletter we present the current knowledge in stem cell research and therapy within the field of orthopaedics, presenting the definitions, types and sources of the stem cells. The second part of this newsletter focuses on the clinical application of stem cells in the therapy of tissues with very limited capacity for self-regeneration; this includes tendons and ligaments, particularly found in rotator cuff rupture...
2015: Casopís Lékar̆ů C̆eských
https://www.readbyqxmd.com/read/26268217/efficacy-of-supraspinatus-tendon-repair-using-mesenchymal-stem-cells-along-with-a-collagen-i-scaffold
#17
Pilar Tornero-Esteban, José Antonio Hoyas, Esther Villafuertes, Cruz Rodríguez-Bobada, Yamila López-Gordillo, Francisco J Rojo, Gustavo V Guinea, Anna Paleczny, Yaiza Lópiz-Morales, Luis Rodriguez-Rodriguez, Fernando Marco, Benjamín Fernández-Gutiérrez
OBJECTIVES: Our main objective was to biologically improve rotator cuff healing in an elderly rat model using mesenchymal stem cells (MSCs) in combination with a collagen membrane and compared against other current techniques. METHODS: A chronic rotator cuff tear injury model was developed by unilaterally detaching the supraspinatus (SP) tendons of Sprague-Dawley rats. At 1 month postinjury, the tears were repaired using one of the following techniques: (a) classical surgery using sutures (n = 12), (b) type I collagen membranes (n = 15), and (c) type I collagen membranes + 1 × 106 allogeneic MSCs (n = 14)...
August 14, 2015: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/26240769/biologic-augmentation-of-the-ulnar-collateral-ligament-in-the-elbow-of-a-professional-baseball-pitcher
#18
James K Hoffman, Nicole M Protzman, Amit D Malhotra
Tears of the ulnar collateral ligament (UCL) of the elbow are common injuries in overhead athletes. Although surgical reconstruction of the UCL has improved outcomes, not all athletes return to their previous level of competition and when this goal is achieved, the time required averages one to two years. Therefore, additional techniques are needed to further improve return to play and the rate of return to play in overhead athletes. A construct comprising a dermal allograft, platelet rich plasma (PRP), and mesenchymal stem cells (MSCs) has been shown to successfully improve healing in the rotator cuff...
2015: Case Reports in Orthopedics
https://www.readbyqxmd.com/read/26224616/stromal-vascular-stem-cell-treatment-decreases-muscle-fibrosis-following-chronic-rotator-cuff-tear
#19
Jonathan P Gumucio, Michael D Flood, Stuart M Roche, Kristoffer B Sugg, Adeyiza O Momoh, Paul E Kosnik, Asheesh Bedi, Christopher L Mendias
PURPOSE: Rotator cuff injuries are associated with atrophy and fat infiltration into the muscle, commonly referred to as "fatty degeneration." As the poor function of chronically torn muscles may limit recovery after surgical repair, there is considerable interest in finding therapies to enhance muscle regeneration. Stromal vascular fraction stem cells (SVFCs) can improve muscle regeneration in other chronic injury states, and our objective was to evaluate the ability of SVFCs to reduce fibrosis and fat accumulation, and enhance muscle fibre specific force production after chronic rotator cuff tear...
April 2016: International Orthopaedics
https://www.readbyqxmd.com/read/26079676/aligned-multilayered-electrospun-scaffolds-for-rotator-cuff-tendon-tissue-engineering
#20
Steven B Orr, Abby Chainani, Kirk J Hippensteel, Alysha Kishan, Christopher Gilchrist, N William Garrigues, David S Ruch, Farshid Guilak, Dianne Little
UNLABELLED: The rotator cuff consists of several tendons and muscles that provide stability and force transmission in the shoulder joint. Whereas most rotator cuff tears are amenable to suture repair, the overall success rate of repair is low, and massive tears are prone to re-tear. Extracellular matrix (ECM) patches are used to augment suture repair, but they have limitations. Tissue-engineered approaches provide a promising solution for massive rotator cuff tears. Previous studies have shown that, compared to nonaligned scaffolds, aligned electrospun polymer scaffolds exhibit greater anisotropy and exert a greater tenogenic effect...
September 2015: Acta Biomaterialia
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