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https://www.readbyqxmd.com/read/29791599/ultra-short-term-heart-rate-variability-during-resistance-exercise-in-the-elderly
#1
G P T Arêas, F C R Caruso, R P Simões, V Castello-Simões, R B Jaenisch, T O Sato, R Cabiddu, R Mendes, R Arena, A Borghi-Silva
Despite the appeal of ultra-short-term heart rate variability (HRV) methods of analysis applied in the clinical and research settings, the number of studies that have investigated HRV by analyzing R-R interval (RRi) recordings shorter than 5 min is still limited. Moreover, ultra-short-term HRV analysis has not been extensively validated during exercise and, currently, no indications exist for its applicability during resistance exercise. The aim of the present study was to compare ultra-short-term HRV analysis with standard short-term HRV analysis during low-intensity, dynamic, lower limb resistance exercise in healthy elderly subjects...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29661824/development-of-dengue-virus-serotype-specific-ns1-capture-assays-for-the-rapid-and-highly-sensitive-identification-of-the-infecting-serotype-in-human-sera
#2
Katharina Röltgen, Natalie Rose, Alessia Ruggieri, Louisa Warryn, Nicole Scherr, Carlos Augusto Pinho-Nascimento, Marco Tamborrini, Thomas Jaenisch, Gerd Pluschke
Dengue fever can be caused by one of four distinct dengue virus (DENV) serotypes that cocirculate in many parts of the world. Point of care serotype-specific nonstructural protein-1 (NS1) capture assays for the rapid serotyping of DENV in human sera would greatly support epidemiological surveillance and potentially also prognosis in individual patients. To ensure both serotype specificity and broad coverage of variants within serotypes, we have applied an innovative approach for the generation and selection of serotype-specific anti-NS1 mAbs...
April 16, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29606614/matched-developmental-timing-of-donor-cells-with-the-host-is-crucial-for-chimera-formation
#3
Malkiel A Cohen, Styliani Markoulaki, Rudolf Jaenisch
Chimeric mice have been generated by injecting pluripotent stem cells into morula-to-blastocyst stage mouse embryo or by introducing more mature cells into later stage embryos that correspond to the differentiation stage of the donor cells. It has not been rigorously tested, however, whether successful chimera formation requires the developmental stage of host embryo and donor cell to be matched. Here, we compared the success of chimera formation following injection of primary neural crest cells (NCCs) into blastocysts or of embryonic stem cells (ESCs) into E8...
March 27, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29599125/establishment-of-human-pluripotent-stem-cell-derived-pancreatic-%C3%AE-like-cells-in-the-mouse-pancreas
#4
Haiting Ma, Katherine J Wert, Dmitry Shvartsman, Douglas A Melton, Rudolf Jaenisch
Type 1 diabetes is characterized by autoimmune destruction of β cells located in pancreatic islets. However, tractable in vivo models of human pancreatic β cells have been limited. Here, we generated xenogeneic human pancreatic β-like cells in the mouse pancreas by orthotopic transplantation of stem cell-derived β (SC-β) cells into the pancreas of neonatal mice. The engrafted β-like cells expressed β cell transcription factors and markers associated with functional maturity. Engrafted human cells recruited mouse endothelial cells, suggesting functional integration...
March 29, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29565043/human-cerebral-organoids-reveal-deficits-in-neurogenesis-and-neuronal-migration-in-mecp2-deficient-neural-progenitors
#5
N Mellios, D A Feldman, S D Sheridan, J P K Ip, S Kwok, S K Amoah, B Rosen, B A Rodriguez, B Crawford, R Swaminathan, S Chou, Y Li, M Ziats, C Ernst, R Jaenisch, S J Haggarty, M Sur
No abstract text is available yet for this article.
April 2018: Molecular Psychiatry
https://www.readbyqxmd.com/read/29554148/cytotoxic-escherichia-coli-strains-encoding-colibactin-isolated-from-immunocompromised-mice-with-urosepsis-and-meningitis
#6
Vasudevan Bakthavatchalu, Katherine J Wert, Yan Feng, Anthony Mannion, Zhongming Ge, Alexis Garcia, Kathleen E Scott, Tyler J Caron, Carolyn M Madden, Johanne T Jacobsen, Gabriel Victora, Rudolf Jaenisch, James G Fox
Immune-compromised mouse models allow for testing the preclinical efficacy of human cell transplantations and gene therapy strategies before moving forward to clinical trials. However, CRISPR/Cas9 gene editing of the Wsh/Wsh mouse strain to create an immune-compromised model lacking function of Rag2 and Il2rγ led to unexpected morbidity and mortality. This warranted an investigation to ascertain the cause and predisposing factors associated with the outbreak. Postmortem examination was performed on 15 moribund mice...
2018: PloS One
https://www.readbyqxmd.com/read/29513667/zika-virus-epidemiology-in-bolivia-a-seroprevalence-study-in-volunteer-blood-donors
#7
Paola Mariela Saba Villarroel, Elif Nurtop, Boris Pastorino, Yelin Roca, Jan Felix Drexler, Pierre Gallian, Thomas Jaenisch, Isabelle Leparc-Goffart, Stéphane Priet, Laetitia Ninove, Xavier de Lamballerie
BACKGROUND: Zika virus (ZIKV), was widely reported in Latin America and has been associated with neuropathologies, as microcephaly, but only few seroprevalence studies have been published to date. Our objective was to determine the seroprevalence amongst Bolivian blood donors and estimate the future potential circulation of the virus. METHODOLOGY: A ZIKV seroprevalence study was conducted between December 2016 and April 2017 in 814 asymptomatic Bolivian volunteer blood donors residing in various eco-environments corresponding to contrasting entomological activities...
March 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29456084/rescue-of-fragile-x-syndrome-neurons-by-dna-methylation-editing-of-the-fmr1-gene
#8
X Shawn Liu, Hao Wu, Marine Krzisch, Xuebing Wu, John Graef, Julien Muffat, Denes Hnisz, Charles H Li, Bingbing Yuan, Chuanyun Xu, Yun Li, Dan Vershkov, Angela Cacace, Richard A Young, Rudolf Jaenisch
Fragile X syndrome (FXS), the most common genetic form of intellectual disability in males, is caused by silencing of the FMR1 gene associated with hypermethylation of the CGG expansion mutation in the 5' UTR of FMR1 in FXS patients. Here, we applied recently developed DNA methylation editing tools to reverse this hypermethylation event. Targeted demethylation of the CGG expansion by dCas9-Tet1/single guide RNA (sgRNA) switched the heterochromatin status of the upstream FMR1 promoter to an active chromatin state, restoring a persistent expression of FMR1 in FXS iPSCs...
February 22, 2018: Cell
https://www.readbyqxmd.com/read/29224777/yy1-is-a-structural-regulator-of-enhancer-promoter-loops
#9
Abraham S Weintraub, Charles H Li, Alicia V Zamudio, Alla A Sigova, Nancy M Hannett, Daniel S Day, Brian J Abraham, Malkiel A Cohen, Behnam Nabet, Dennis L Buckley, Yang Eric Guo, Denes Hnisz, Rudolf Jaenisch, James E Bradner, Nathanael S Gray, Richard A Young
There is considerable evidence that chromosome structure plays important roles in gene control, but we have limited understanding of the proteins that contribute to structural interactions between gene promoters and their enhancer elements. Large DNA loops that encompass genes and their regulatory elements depend on CTCF-CTCF interactions, but most enhancer-promoter interactions do not employ this structural protein. Here, we show that the ubiquitously expressed transcription factor Yin Yang 1 (YY1) contributes to enhancer-promoter structural interactions in a manner analogous to DNA interactions mediated by CTCF...
December 14, 2017: Cell
https://www.readbyqxmd.com/read/29166608/s-nitrosylation-of-pink1-attenuates-pink1-parkin-dependent-mitophagy-in-hipsc-based-parkinson-s-disease-models
#10
Chang-Ki Oh, Abdullah Sultan, Joseph Platzer, Nima Dolatabadi, Frank Soldner, Daniel B McClatchy, Jolene K Diedrich, John R Yates, Rajesh Ambasudhan, Tomohiro Nakamura, Rudolf Jaenisch, Stuart A Lipton
Mutations in PARK6 (PINK1) and PARK2 (Parkin) are linked to rare familial cases of Parkinson's disease (PD). Mutations in these genes result in pathological dysregulation of mitophagy, contributing to neurodegeneration. Here, we report that environmental factors causing a specific posttranslational modification on PINK1 can mimic these genetic mutations. We describe a molecular mechanism for impairment of mitophagy via formation of S-nitrosylated PINK1 (SNO-PINK1). Mitochondrial insults simulating age- or environmental-related stress lead to increased SNO-PINK1, inhibiting its kinase activity...
November 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/29138300/high-zika-virus-seroprevalence-in-salvador-northeastern-brazil-limits-the-potential-for-further-outbreaks
#11
Eduardo Martins Netto, Andres Moreira-Soto, Celia Pedroso, Christoph Höser, Sebastian Funk, Adam J Kucharski, Alexandra Rockstroh, Beate M Kümmerer, Gilmara Souza Sampaio, Estela Luz, Sara Nunes Vaz, Juarez Pereira Dias, Fernanda Anjos Bastos, Renata Cabral, Thomas Kistemann, Sebastian Ulbert, Xavier de Lamballerie, Thomas Jaenisch, Oliver J Brady, Christian Drosten, Manoel Sarno, Carlos Brites, Jan Felix Drexler
During 2015 to 2016, Brazil reported more Zika virus (ZIKV) cases than any other country, yet population exposure remains unknown. Serological studies of ZIKV are hampered by cross-reactive immune responses against heterologous viruses. We conducted serosurveys for ZIKV, dengue virus (DENV), and Chikungunya virus (CHIKV) in 633 individuals prospectively sampled during 2015 to 2016, including microcephaly and non-microcephaly pregnancies, HIV-infected patients, tuberculosis patients, and university staff in Salvador in northeastern Brazil using enzyme-linked immunosorbent assays (ELISAs) and plaque reduction neutralization tests...
November 14, 2017: MBio
https://www.readbyqxmd.com/read/29113152/effect-of-surgical-margins-on-prognosis-in-aggressive-fibromatosis-a-single-institutional-analysis-of-90-patients
#12
Kamran Harati, Anais Jaenisch, Björn Behr, Ole Goertz, Ali Harati, Tobias Hirsch, Ingo Stricker, Marcus Lehnhardt, Adrien Daigeler
The treatment of aggressive fibromatosis poses a therapeutic challenge in an interdisciplinary setting. The extent of surgical resection is still discussed controversially. The present retrospective analysis aimed to determine prognostic factors leading to recurrence. Between 2000 and 2014, 114 patients with aggressive fibromatosis were treated surgically at BG-University Hospital Bergmannsheil (Bochum, Germany). Univariate and multivariate analyses were restricted to 90 participants with information available on surgical margins at the initial procedure...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29108009/zika-virus-displacement-by-a-chikungunya-outbreak-in-recife-brazil
#13
Tereza Magalhaes, Cynthia Braga, Marli T Cordeiro, Andre L S Oliveira, Priscila M S Castanha, Ana Paula R Maciel, Nathalia M L Amancio, Pollyanne N Gouveia, Valter J Peixoto-da-Silva, Thaciana F L Peixoto, Helena Britto, Priscilla V Lima, Andreza R S Lima, Kerstin D Rosenberger, Thomas Jaenisch, Ernesto T A Marques
BACKGROUND: Several arboviruses, including dengue virus (DENV), Zika virus (ZIKV) and chikungunya virus (CHIKV), transmitted by Aedes mosquitoes, circulate in northeast Brazil. Diseases caused by these viruses are of great public health relevance, however, their epidemiological features in areas where the three viruses co-circulate are scarce. Here, we present analyses of molecular and serological diagnostics in a prospective study of acute febrile patients recruited from May 2015 to May 2016 in Recife, Brazil...
November 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29061959/dynamics-of-lineage-commitment-revealed-by-single-cell-transcriptomics-of-differentiating-embryonic-stem-cells
#14
Stefan Semrau, Johanna E Goldmann, Magali Soumillon, Tarjei S Mikkelsen, Rudolf Jaenisch, Alexander van Oudenaarden
Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here we measure the gene expression dynamics of retinoic acid driven mESC differentiation from pluripotency to lineage commitment, using an unbiased single-cell transcriptomics approach. We find that the exit from pluripotency marks the start of a lineage transition as well as a transient phase of increased susceptibility to lineage specifying signals...
October 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28987235/effects-of-l-arginine-supplementation-associated-with-continuous-or-interval-aerobic-training-on-chronic-heart-failure-rats
#15
Giovanna Tedesco Barcelos, Douglas Dalcin Rossato, Júlia Luiza Perini, Lucas Pereira Pinheiro, Carol Carvalho, Rodrigo Boemo Jaenisch, Cláudia Ramos Rhoden, Pedro Dal Lago, Ramiro Barcos Nunes
OBJECTIVE: Chronic heart failure (CHF) is related with exercise intolerance and impaired nitric oxide (NO) production, which can lead to several functional capacity alterations. Considering the possible superiority of aerobic interval training compared to continuous training and the capacity of l-arginine to restore the NO pathway, the aim of the present study was to investigate whether these treatments are beneficial to exercise capacity, muscle mass preservation and hemodynamic, inflammatory and oxidative stress parameters in CHF rats...
November 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28985523/human-embryo-editing-opportunities-and-importance-of-transnational-cooperation
#16
Duanqing Pei, David W Beier, Ephrat Levy-Lahad, Gary Marchant, Janet Rossant, Juan Carlos Izpisua Belmonte, Robin Lovell-Badge, Rudolf Jaenisch, Alta Charo, David Baltimore
A recent National Academies report articulates a path forward for research, ethics, and governance of clinical applications involving genome editing. In light of recent human embryo editing developments, scientists and stakeholders from all nations should cooperate to take advantage of this historic opportunity for medicine and also basic human biology.
October 5, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28966122/an-endogenously-tagged-fluorescent-fusion-protein-library-in-mouse-embryonic-stem-cells
#17
Arigela Harikumar, Raghu Ram Edupuganti, Matan Sorek, Gajendra Kumar Azad, Styliani Markoulaki, Petra Sehnalová, Soňa Legartová, Eva Bártová, Shlomit Farkash-Amar, Rudolf Jaenisch, Uri Alon, Eran Meshorer
Embryonic stem cells (ESCs), with their dual capacity to self-renew and differentiate, are commonly used to study differentiation, epigenetic regulation, lineage choices, and more. Using non-directed retroviral integration of a YFP/Cherry exon into mouse ESCs, we generated a library of over 200 endogenously tagged fluorescent fusion proteins and present several proof-of-concept applications of this library. We show the utility of this library to track proteins in living cells; screen for pluripotency-related factors; identify heterogeneously expressing proteins; measure the dynamics of endogenously labeled proteins; track proteins recruited to sites of DNA damage; pull down tagged fluorescent fusion proteins using anti-Cherry antibodies; and test for interaction partners...
October 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28966118/alternative-set-tafi-promoters-regulate-embryonic-stem-cell-differentiation
#18
Raghu Ram Edupuganti, Arigela Harikumar, Yair Aaronson, Alva Biran, Badi Sri Sailaja, Malka Nissim-Rafinia, Gajendra Kumar Azad, Malkiel A Cohen, Jung Eun Park, Chikdu S Shivalila, Styliani Markoulaki, Siu Kwan Sze, Rudolf Jaenisch, Eran Meshorer
Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters...
October 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28620698/potential-disease-biomarkers-dipeptidyl-peptidase-4-and-fibroblast-activation-protein
#19
REVIEW
Roger Yazbeck, Simone E Jaenisch, Catherine A Abbott
The importance of the dipeptidyl peptidase 4 (DPP4) gene family in regulating critical biochemical pathways continues to emerge. The two most well-studied members of the family, DPP4 and fibroblast activation protein (FAP), have been investigated both as therapeutic targets for disease and as diagnostic biomarkers. The interest in DPP4 and FAP as potential disease biomarkers has been driven primarily by observations of altered expression profiles in inflammatory diseases and cancer. Furthermore, the stability and persistence of soluble DPP4 and FAP in the serum make them attractive candidate serology markers...
January 2018: Protoplasma
https://www.readbyqxmd.com/read/28619162/impact-of-treatments-for-recycled-broiler-litter-on-the-viability-and-infectivity-of-microorganisms
#20
Daiane Voss-Rech, Iara Maria Trevisol, Liana Brentano, Virgínia Santiago Silva, Raquel Rebelatto, Fátima Regina Ferreira Jaenisch, Cintia Hiromi Okino, Marcos Antonio Zanella Mores, Arlei Coldebella, Sônia de Avila Botton, Clarissa Silveira Luiz Vaz
The microbiological risk of recycled litter depends on the efficacy of the management system applied to inactivate residual microorganisms and preserve the health of the successive broiler flock. This study aimed to assess the viability and infectivity of the Newcastle Disease Virus (NDV), Infectious Bursal Disease Virus (IBDV) and Salmonella Heidelberg in recycled litter exposed to different treatments. The litter was contaminated with microorganisms and submitted to the treatments (T): T1: shallow fermentation; T2: quicklime (calcium oxide); T3: shallow fermentation followed by addition of quicklime; T4: no treatment...
May 2017: Veterinary Microbiology
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