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https://www.readbyqxmd.com/read/28944538/mumads1-and-maofp1-regulate-fruit-quality-in-a-tomato-ovate-mutant
#1
Juhua Liu, Jing Zhang, Jingyi Wang, Jianbin Zhang, Hongxia Miao, Caihong Jia, Zhuo Wang, Biyu Xu, Zhiqiang Jin
Fruit ripening and quality are common botanical phenomena that are closely linked and strictly regulated by transcription factors. It was previously discovered that a banana MADS-box protein named MuMADS1 interacted with an ovate family protein named MaOFP1 to regulate banana fruit ripening. In order to further investigate the role of MuMADS1 and MaOFP1 in the regulation of fruit quality, a combination of genetic transformation and transcriptional characterization was used. The results indicated that the co-expression of MuMADS1 and MaOFP1 in the ovate mutant could compensate for fruit shape and inferior qualities relating to fruit firmness, soluble solids and sugar content...
September 25, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28943832/identification-of-candidate-genes-involved-in-the-sugar-metabolism-and-accumulation-during-pear-fruit-post-harvest-ripening-of-red-clapp-s-favorite-pyrus-communis-l-by-transcriptome-analysis
#2
Long Wang, Yun Chen, Suke Wang, Huabai Xue, Yanli Su, Jian Yang, Xiugen Li
BACKGROUND: Pear (Pyrus spp.) is a popular fruit that is commercially cultivated in most temperate regions. In fruits, sugar metabolism and accumulation are important factors for fruit organoleptic quality. Post-harvest ripening is a special feature of 'Red Clapp's Favorite'. RESULTS: In this study, transcriptome sequencing based on the Illumina platform generated 23.8 - 35.8 million unigenes of nine cDNA libraries constructed using RNAs from the 'Red Clapp's Favorite' pear variety with different treatments, in which 2629 new genes were discovered, and 2121 of them were annotated...
2018: Hereditas
https://www.readbyqxmd.com/read/28942290/changing-of-biochemical-parameters-and-cell-wall-polysaccharides-distribution-during-physiological-development-of-tomato-fruit
#3
Monika Chylińska, Monika Szymańska-Chargot, Kamil Deryło, Dorota Tchórzewska, Artur Zdunek
This study was aimed at discovering an impact of biochemical parameters (like content of cell wall polysaccharides, phenolic compounds, ascorbic acid or activity of pectinolytic enzymes) on cell wall microstructure during physiological fruit development. Cell wall microstructure as well as changes in the polysaccharides distribution were examined by confocal Raman microscopy. Also there was a need to simultaneous usage of reference method which is immunolabeling. A tomato fruit (Solanum lycopersicum cv Cerise) has been selected to observe the changes taking place in the fruit cell wall as it recently has been recognized as a model species for exploring fruit development processes such as fruit formation and ripening...
September 14, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28941905/redefining-the-effect-of-salt-on-thermophilic-starter-cell-viability-culturability-and-metabolic-activity-in-cheese
#4
C D Hickey, V Fallico, M G Wilkinson, J J Sheehan
This study investigated the differential effect of salt concentration in the outside and inside layers of brine salted cheeses on viability, culturability and enzyme activity of starter bacteria. The high-salt environment of the outside layer caused a sharp decrease in L. helveticus viability as measured by traditional plate counts. Remarkably, this was associated with lower release of intracellular enzymes (LDH), reduced levels of proteolysis and larger membrane integrity as measured by flow cytometry (FC) following classical Live/Dead staining...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28941817/evolution-of-free-amino-acids-during-ripening-of-caciocavallo-cheeses-made-with-different-milks
#5
Serena Niro, Mariantonietta Succi, Patrizio Tremonte, Elena Sorrentino, Raffaele Coppola, Gianfranco Panfili, Alessandra Fratianni
The evolution of free amino acids (FAA) in Caciocavallo cheeses, made with cow milk (CC) and cow milk mixed with ewe (CE) and goat (CG) milk, was studied throughout ripening. In all Caciocavallo cheeses produced, the total free amino acid (TFAA) content increased during ripening. In general, the highest TFAA content was found in cow cheeses, and the lowest in CG cheeses, whereas CE cheeses ranged over an intermediate level. In all the analyzed samples, during ripening, the content of the individual FAA increased with the exception of arginine...
September 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28941248/lipolysis-and-aroma-generation-as-mechanisms-involved-in-masking-boar-taint-in-sodium-reduced-fermented-sausages-inoculated-with-d-hansenii-yeast
#6
Sara Corral, Carmela Belloch, José J López-Díez, Mónica Flores
BACKGROUND: The use of boar back fat for processing of fermented sausages may cause the presence of abnormal odours. In dry-cured products, ripening time is essential to develop the sensory characteristics. Yeast has been proposed as an alternative to mask boar taint odour through its metabolic activity but it is necessary to elucidate which mechanisms are involved. The aim is to study the effect of D. hansenii inoculation on the lipolysis process and generation of aroma compounds in fermented sausages manufactured with boar back fat at two different ripening times...
September 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28939933/cellular-mechanics-of-primary-human-cervical-fibroblasts-influence-of-progesterone-and-a-pro-inflammatory-cytokine
#7
Vasudha Shukla, Victoria Barnhouse, William E Ackerman, Taryn L Summerfield, Heather M Powell, Jennifer L Leight, Douglas A Kniss, Samir N Ghadiali
The leading cause of neonatal mortality, pre-term birth, is often caused by pre-mature ripening/opening of the uterine cervix. Although cervical fibroblasts play an important role in modulating the cervix's extracellular matrix (ECM) and mechanical properties, it is not known how hormones, i.e., progesterone, and pro-inflammatory insults alter fibroblast mechanics, fibroblast-ECM interactions and the resulting changes in tissue mechanics. Here we investigate how progesterone and a pro-inflammatory cytokine, IL-1β, alter the biomechanical properties of human cervical fibroblasts and the fibroblast-ECM interactions that govern tissue-scale mechanics...
September 22, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28936215/omics-approaches-for-understanding-grapevine-berry-development-regulatory-networks-associated-with-endogenous-processes-and-environmental-responses
#8
REVIEW
Alejandra Serrano, Carmen Espinoza, Grace Armijo, Claudio Inostroza-Blancheteau, Evelyn Poblete, Carlos Meyer-Regueiro, Anibal Arce, Francisca Parada, Claudia Santibáñez, Patricio Arce-Johnson
Grapevine fruit development is a dynamic process that can be divided into three stages: formation (I), lag (II), and ripening (III), in which physiological and biochemical changes occur, leading to cell differentiation and accumulation of different solutes. These stages can be positively or negatively affected by multiple environmental factors. During the last decade, efforts have been made to understand berry development from a global perspective. Special attention has been paid to transcriptional and metabolic networks associated with the control of grape berry development, and how external factors affect the ripening process...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28935987/toward-the-identification-of-a-type-i-toxin-antitoxin-system-in-the-plasmid-dna-of-dairy-lactobacillus-rhamnosus
#9
Claudia Folli, Alessia Levante, Riccardo Percudani, Davide Amidani, Stefania Bottazzi, Alberto Ferrari, Claudio Rivetti, Erasmo Neviani, Camilla Lazzi
Plasmids carry genes that give bacteria beneficial traits and allow them to survive in competitive environments. In many cases, they also harbor toxin-antitoxin (TA) systems necessary for plasmid maintenance. TA systems are generally characterized by a stable "toxin", a protein or peptide capable of killing the cell upon plasmid loss and by an unstable "antitoxin", a protein or a non-coding RNA that inhibits toxin activity. Here we report data toward the identification of a RNA-regulated TA system in the plasmid DNA of L...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28932910/a-histone-deacetylase-gene-slhda3-acts-as-a-negative-regulator-of-fruit-ripening-and-carotenoid-accumulation
#10
Jun-E Guo, Zongli Hu, Xiaohui Yu, Anzhou Li, Fenfen Li, Yunshu Wang, Shibing Tian, Guoping Chen
SlHDA3 functions as an inhibitor and regulates tomato fruit ripening and carotenoid accumulation. Post-translational modifications, including histones acetylation, play a pivotal role in the changes of chromatin structure dynamic modulation and gene activity. The regulation of histone acetylation is achieved by the action of histone acetyltransferases and deacetylases, which play crucial roles in the regulation of transcription activation. There is an increasing research focus on histone deacetylation in crops, but the role of histone deacetylase genes (HDACs) in tomato has not been elucidated...
September 20, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28928762/novel-protein-protein-inhibitor-based-approach-to-control-plant-ethylene-responses-synthetic-peptides-for-ripening-control
#11
Mareike Kessenbrock, Simone M Klein, Lena Müller, Mauricio Hunsche, Georg Noga, Georg Groth
Ethylene signaling is decisive for many plant developmental processes. Among these, control of senescence, abscission and fruit ripening are of fundamental relevance for global agriculture. Consequently, detailed knowledge of the signaling network along with the molecular processes of signal perception and transfer are expected to have high impact on future food production and agriculture. Recent advances in ethylene research have demonstrated that signaling of the plant hormone critically depends on the interaction of the ethylene receptor family with the NRAMP-like membrane protein ETHYLENE INSENSITIVE 2 (EIN2) at the ER membrane, phosphorylation-dependent proteolytic processing of ER-localized EIN2 and subsequent translocation of the cleaved EIN2 C-terminal polypeptide (EIN2-CEND) to the nucleus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28928755/different-preclimacteric-events-in-apple-cultivars-with-modified-ripening-physiology
#12
Vikram Singh, Asya Weksler, Haya Friedman
"Anna" is an early season apple cultivar exhibiting a fast softening and juiciness loss during storage, in comparison to two mid-late season cultivars "Galaxy" and "GD." The poor storage capacity of "Anna" was correlated with high lipid oxidation-related autoluminescence, high respiration and ethylene production rates, associated with high expression of MdACO1, 2, 4, 7, and MdACS1. All cultivars at harvest responded to exogenous ethylene by enhancing ethylene production, typical of system-II. The contribution of pre-climacteric events to the poor storage capacity of "Anna" was examined by comparing respiration and ethylene production rates, response to exogenous ethylene, expression of genes responsible for ethylene biosynthesis and response, and developmental regulators in the three cultivars throughout fruit development...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28928519/measurement-of-ripening-of-raspberries-rubus-idaeus-l-by-near-infrared-and-colorimetric-imaging-techniques
#13
Francisco J Rodríguez-Pulido, María Gil-Vicente, Belén Gordillo, Francisco J Heredia, M Lourdes González-Miret
This work includes the evaluation of 168 samples of raspberries 'Glen Lyon', representing whole maturation period, by colorimetric and near infrared imaging techniques, as well as the quantification of total phenols, total anthocyanins and antioxidant activity by chemical methods. Samples showed significant differences depending on the maturation stage using CIELAB colour parameters and total anthocyanins content. The application of partial least squares regression allowed predicting the chemical features from image analysis data, with coefficients of determination (R(2)) up to 0...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28926798/cloning-and-expression-profiling-of-the-pacsnrk2-and-pacpp2c-gene-families-during-fruit-development-aba-treatment-and-dehydration-stress-in-sweet-cherry
#14
Xinjie Shen, Xiao Guo, Di Zhao, Qiang Zhang, Yuzhuang Jiang, Yantao Wang, Xiang Peng, Yan Wei, Zefeng Zhai, Wei Zhao, Tianhong Li
Plant SNF1-related protein kinase 2 (SnRK2) and protein phosphatase 2C (PP2C) family members are core components of the ABA signal transduction pathway. SnRK2 and PP2C proteins have been suggested to play crucial roles in fruit ripening and improving plant tolerance to drought stress, but supporting genetic information has been lacking in sweet cherry (Prunus avium L.). Here, we cloned six full-length SnRK2 genes and three full-length PP2C genes from sweet cherry cv. Hong Deng. Quantitative PCR analysis revealed that PacSnRK2...
September 6, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28923659/corrigendum-to-short-communication-survival-of-vaccinia-virus-in-inoculated-cheeses-during-60-day-ripening-j-dairy-sci-100-7051-7054
#15
Izabelle S Rehfeld, Ana Luiza S Fraiha, Ana Carolina D Matos, Maria Isabel M C Guedes, Erica A Costa, Marcelo R de Souza, Luigi F L Cavalcante, Zélia I P Lobato
No abstract text is available yet for this article.
October 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28922568/spectral-libraries-for-swath-ms-assays-for-drosophila-melanogaster-and-solanum-lycopersicum
#16
Bertrand Fabre, Dagmara Korona, Clara I Mata-Martinez, Harriet T Parsons, Michael J Deery, Maarten L A T M Hertog, Bart M Nicolaï, Steven Russell, Kathryn S Lilley
Quantitative proteomics methods have emerged as powerful tools for measuring protein expression changes at the proteome level. Using mass-spectrometry (MS) based approaches, it is now possible to routinely quantify thousands of proteins. However, pre-fractionation of the samples at the protein or peptide level is usually necessary to go deep into the proteome, increasing both MS analysis time and technical variability. Recently, a new MS acquisition method named SWATH was introduced with the potential to provide good coverage of the proteome as well as a good measurement precision without prior sample fractionation...
September 18, 2017: Proteomics
https://www.readbyqxmd.com/read/28920504/outpatient-cervical-ripening-in-a-district-general-hospital-a-five-year-retrospective-cohort-study
#17
Lauren Barnfield, Edmund Neale, Sarah Reynolds
The number of women undergoing induction of labour has risen steadily in recent years. Outpatient induction is becoming more common in the UK in response to the required increase in resources, although evidence supporting its safety is lacking. We reviewed the notes of low-risk women presenting for outpatient cervical ripening using prostaglandins over a five-year period, and compared our neonatal and maternal outcomes to local and national data. Of the 502 eligible women, 400 underwent outpatient treatment...
September 18, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28919902/expression-differences-of-pigment-structural-genes-and-transcription-factors-explain-flesh-coloration-in-three-contrasting-kiwifruit-cultivars
#18
Yanfei Liu, Bin Zhou, Yingwei Qi, Xin Chen, Cuihua Liu, Zhande Liu, Xiaolin Ren
Fruits of kiwifruit cultivars (Actinidia chinensis and A. deliciosa) generally have green or yellow flesh when ripe. A small number of genotypes have red flesh but this coloration is usually restricted to the inner pericarp. Three kiwifruit cultivars having red ('Hongyang'), or yellow ('Jinnong-2'), or green ('Hayward') flesh were investigated for their color characteristics and pigment contents during development and ripening. The results show the yellow of the 'Jinnong-2' fruit is due to the combined effects of chlorophyll degradation and of beta-carotene accumulation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28918545/increased-postharvest-life-of-tomlox-b-silenced-mutants-of-tomato-solanum-lycopersicum-var-ta234
#19
Elizabeth León-García, Gilber Vela-Gutiérrez, Oscar A Del Ángel-Coronel, Cristobal Torres-Palacios, Javier De La Cruz-Medina, Miguel A Gómez-Lim, Hugo Sergio García
A healthy lifestyle includes fruits and vegetables consumption. Tomato is one of the most consumed vegetables, although it is susceptible to physical damage through postharvest handling, thus leading to important losses. Softening is an important variable during tomato ripening; excessive softening is undesirable and leads to postharvest losses. TomloxB plays an important role in ripening, mainly in the loss of cellular integrity caused by fatty acids released from the lipid matrix of membranes that initiate oxidative deterioration, which is in turn carried into senescence...
September 16, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/28918140/technical-note-feasibility-of-near-infrared-transmittance-spectroscopy-to-predict-cheese-ripeness
#20
S Currò, C L Manuelian, M Penasa, M Cassandro, M De Marchi
The aim of the study was to evaluate the feasibility of near infrared (NIR) transmittance spectroscopy to predict cheese ripeness using the ratio of water-soluble nitrogen (WSN) to total nitrogen (TN) as an index of cheese maturity (WSN/TN). Fifty-two Protected Designation of Origin cow milk cheeses of 5 varieties (Asiago, Grana Padano, Montasio, Parmigiano Reggiano, and Piave) and different ripening times were available for laboratory and chemometric analyses. Reference measures of WSN and TN were matched with cheese spectral information obtained from ground samples by a NIR instrument that operated in transmittance mode for wavelengths from 850 to 1,050 nm...
September 13, 2017: Journal of Dairy Science
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