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https://www.readbyqxmd.com/read/28737900/accumulation-of-charantin-and-expression-of-triterpenoid-biosynthesis-genes-in-bitter-melon-momordica-charantia
#1
Do Manh Cuong, Jin Jeon, Abubaker M A Morgan, Changsoo Kim, Jae Kwang Kim, Sook Young Lee, Sang Un Park
Charantin, a natural cucurbitane type triterpenoid, has been reported to have beneficial pharmacological functions such as anticancer, antidiabetic, and antibacterial activities. However, accumulation of charantin in bitter melon has been little studied. Here, we performed a transcriptome analysis to identify genes involved in the triterpenoid biosynthesis pathway in bitter melon seedlings. Total of 88,703 transcripts with an average length of 898 bp were identified in bitter melon seedlings. Based on a functional annotation, we identified 15 candidate genes encoding enzymes related to triterpenoid biosynthesis and analyzed their expression in different organs of mature plants...
July 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28735145/metabolic-gene-targeted-monitoring-of-non-starter-lactic-acid-bacteria-during-cheese-ripening
#2
Alessia Levante, Francesca De Filippis, Antonietta La Storia, Monica Gatti, Erasmo Neviani, Danilo Ercolini, Camilla Lazzi
Long ripened cheeses, such as Grana Padano (GP), a Protected Designation of Origin (PDO) Italian cheese, harbor a viable microbiota mainly composed of non-starter lactic acid bacteria (NSLAB), which contribute to the final characteristics of cheese. The NSLAB species Lactobacillus rhamnosus, Lb. casei and Lb. paracasei are frequently found in GP, and form a closely related taxonomic group (Lb. casei group), making it difficult to distinguish the three species through 16S rRNA sequencing. SpxB, a metabolic gene coding for pyruvate oxidase in Lb...
July 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28734599/short-communication-survival-of-vaccinia-virus-in-inoculated-cheeses-during-60-day-ripening
#3
Izabelle S Rehfeld, Ana Luiza S Fraiha, Ana Carolina D Matos, Maria Isabel M C Guedes, Erica A Costa, Marcelo R de Souza, Luigi F L Cavalcante, Zélia I P Lobato
Bovine vaccinia is a neglected zoonosis caused by Vaccinia virus (VACV) and has a major economic and public health effect in Brazil. Previous studies showed infectious VACV particles in milk from either experimentally or naturally infected cows and in fresh cheeses prepared with experimentally contaminated milk. Ripening is a process that leads to major changes in the physical and chemical characteristics of cheese, reducing contamination by spoilage, pathogenic microorganisms, or both. However, it is not known if VACV infectious particles persist after the ripening process...
July 19, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28733102/transcriptome-landscape-of-human-primary-monocytes-at-different-sequencing-depth
#4
Hoda Mirsafian, Adiratna Mat Ripen, Wai-Mun Leong, Thamilvaani Manaharan, Saharuddin Bin Mohamad, Amir Feisal Merican
Differential gene and transcript expression pattern of human primary monocytes from healthy young subjects were profiled under different sequencing depths (50M, 100M, and 200M reads). The raw data consisted of 1.3 billion reads generated from RNA sequencing (RNA-Seq) experiments. A total of 17,657 genes and 75,392 transcripts were obtained at sequencing depth of 200M. Total splice junction reads showed an even more significant increase. Comparative analysis of the expression patterns of immune-related genes revealed a total of 217 differentially expressed (DE) protein-coding genes and 50 DE novel transcripts, in which 40 DE protein-coding genes were related to the immune system...
July 18, 2017: Genomics
https://www.readbyqxmd.com/read/28728356/antioxidant-potential-of-cheddar-cheeses-to-tackle-human-colon-adenocarcinoma-caco-2-cells
#5
Nuzhat Huma, Saima Rafiq, Aysha Sameen, Imran Pasha, Muhammad Issa Khan
Objective: Increased production of reactive oxygen species (ROS) affects gastrointestinal system which may develop intestinal pathologies. Food resources comprising natural antioxidants with chemotherapeutic properties have greater demand in current marketplace Therefore, aim of present study was to assess the anti-oxidant potential of water-soluble peptides (WSPs) extract derived from buffalo and cow milk Cheddar cheeses at different stages of ripening. Methods: The antioxidant potential of WSPs extract was assessed through ABTS-radical scavenging activity...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28724949/overexpression-of-slpre2-an-atypical-bhlh-transcription-factor-affects-plant-morphology-and-fruit-pigment-accumulation-in-tomato
#6
Zhiguo Zhu, Guoping Chen, Xuhu Guo, Wencheng Yin, Xiaohui Yu, Jingtao Hu, Zongli Hu
The basic helix-loop-helix (bHLH) proteins are a large family of transcription factors that control various developmental processes in eukaryotes, but the biological roles of most bHLH proteins are not very clear, especially in tomato. In this study, a PRE-like atypical bHLH gene was isolated and designated as SlPRE2 in tomato. SlPRE2 was highly expressed in immature-green fruits, moderately in young leaves, flowers, and mature-green fruits. To further research the function of SlPRE2, a 35 S:PRE2 binary vector was constructed and transformed into wild type tomato...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28722752/application-of-a-sensory-instrumental-tool-to-study-apple-texture-characteristics-shaped-by-altitude-and-time-of-harvest
#7
Mathilde Charles, Maria Laura Corollaro, Luigi Manfrini, Isabella Endrizzi, Eugenio Aprea, Angelo Zanella, Luca Corelli Grappadelli, Flavia Gasperi
BACKGROUND: Texture is important in the preferences of apple consumers. Of the pre-harvest factors affecting fruit quality and especially texture, altitude and subsequent climatic conditions are crucial, determining differences in the physiological mechanisms of fruit growth, ripening stage and chemical composition, as demonstrated by several studies. This work applies a detailed sensory-instrumental protocol developed in a previous paper(1) to investigate the impact of altitude, time of harvest and their cross-effect on sensory characteristics of apple, with a focus on texture...
July 19, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28721852/effective-identification-of-lactobacillus-casei-group-species-genome-based-selection-of-the-gene-mutl-as-the-target-of-a-novel-multiplex-pcr-assay
#8
Benedetta Bottari, Giovanna E Felis, Elisa Salvetti, Anna Castioni, Ilenia Campedelli, Sandra Torriani, Valentina Bernini, Monica Gatti
Lactobacillus casei,Lactobacillus paracasei and Lactobacillusrhamnosus form a closely related taxonomic group (the L. casei group) within the facultatively heterofermentative lactobacilli. Strains of these species have been used for a long time as probiotics in a wide range of products, and they represent the dominant species of nonstarter lactic acid bacteria in ripened cheeses, where they contribute to flavour development. The close genetic relationship among those species, as well as the similarity of biochemical properties of the strains, hinders the development of an adequate selective method to identify these bacteria...
July 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28720953/physico-chemical-changes-during-progressive-ripening-of-mango-mangifera-indica-l-cv-dashehari-under-different-temperature-regimes
#9
P P S Gill, S K Jawandha, Navdeep Kaur, Navprem Singh
The effect of different temperature regimes on ripening quality of mango cv. Dashehari was investigated. Fruits were kept at 20, 25 °C in temperature controlled chambers and at room temperature. Fruits were analyzed periodically for physico-chemical characteristics after every 24 h interval up to 168 h of ripening period. Development of soluble solid contents (SSC), flesh softening and physiological loss of weight of fruits occurred progressively during ripening. Progression of ripening changes in fruit were found to be less at 20 and 25 °C than at room temperature...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720774/a-novel-fluorescent-probe-based-flow-cytometric-assay-for-mineral-containing-nanoparticles-in-serum
#10
Edward R Smith, Tim D Hewitson, Michael M X Cai, Parisa Aghagolzadeh, Matthias Bachtler, Andreas Pasch, Stephen G Holt
Calciprotein particles, nanoscale aggregates of insoluble mineral and binding proteins, have emerged as potential mediators of phosphate toxicity in patients with Chronic Kidney Disease. Although existing immunochemical methods for their detection have provided compelling data, these approaches are indirect, lack specificity and are subject to a number of other technical and theoretical shortcomings. Here we have developed a rapid homogeneous fluorescent probe-based flow cytometric method for the detection and quantitation of individual mineral-containing nanoparticles in human and animal serum...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720093/solanum-nigrum-unripe-fruit-fraction-attenuates-adriamycin-resistance-by-down-regulating-multi-drug-resistance-protein-mdr-1-through-jak-stat-pathway
#11
Sankar Jagadeeshan, Diana David, S Jisha, S Manjula, S Asha Nair
BACKGROUND: Solanum nigrum, herbal plant that commonly grows in temperate climate zone, has been used as a traditional folk medicine whose ripen fruits were proven to exhibit anti-tumor properties. In traditional Chinese medicine, it has been used for centuries to cure inflammation, edema, mastitis and hepatic cancer and in the Ayurvedic system of traditional medicine in India, S. nigrum is applied against enteric diseases, ulcer, diarrhea and skin diseases. A methanolic glycosidic extract fraction of unripe fruit of S...
July 18, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28716915/prostaglandin-dehydrogenase-is-a-target-for-successful-induction-of-cervical-ripening
#12
Annavarapu Hari Kishore, Hanquan Liang, Mohammed Kanchwala, Chao Xing, Thota Ganesh, Yucel Akgul, Bruce Posner, Joseph M Ready, Sanford D Markowitz, Ruth Ann Word
The cervix represents a formidable structural barrier for successful induction of labor. Approximately 10% of pregnancies undergo induction of cervical ripening and labor with prostaglandin (PG) E2 or PGE analogs, often requiring many hours of hospitalization and monitoring. On the other hand, preterm cervical ripening in the second trimester predicts preterm birth. The regulatory mechanisms of this paradoxical function of the cervix are unknown. Here, we show that PGE2 uses cell-specific EP2 receptor-mediated increases in Ca(2+) to dephosphorylate and translocate histone deacetylase 4 (HDAC4) to the nucleus for repression of 15-hydroxy prostaglandin dehydrogenase (15-PGDH)...
July 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28716425/characterization-and-quantification-of-flavonoids-and-organic-acids-over-fruit-development-in-american-cranberry-vaccinium-macrocarpon-cultivars-using-hplc-and-apci-ms-ms
#13
Yifei Wang, Jennifer Johnson-Cicalese, Ajay P Singh, Nicholi Vorsa
Cranberry flavonoids, including anthocyanins, flavonol glycosides and proanthocyanidins, and organic acids were characterized and quantified by HPLC and LC-MS/MS during fruit development and ripening in eight cranberry cultivars. Anthocyanin biosynthesis initiated at early fruit development and reached highest level in mature fruit, with significant differences between cultivars. Major flavonol glycosides, including the most abundant quercetin-3-galactoside and myricetin-3-galactoside, showed consistent concentrations during the season with moderate fluctuation, and were at similar levels in mature fruits of the eight cultivars...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28714250/development-of-antimicrobial-nanoemulsion-based-delivery-systems-against-selected-pathogenic-bacteria-using-a-thymol-rich-thymus-daenensis-essential-oil
#14
Lida Ghaderi, Roya Moghimi, Atousa Aliahmadi, David Julian McClements, Hasan Rafati
AIMS: Thymol rich medicinal plants have been used in traditional medicine to relieve infectious diseases. However, the application of essential oils as medicine is limited by its low water-solubility and high vapor pressure. The objective of this study was to produce stable nanoemulsions of Thymus daenensis oil in water by preventing Ostwald ripening and phase separation. METHODS & RESULTS: The antibacterial activity of bulk and emulsified essential oil against selected pathogenic bacteria including Gram-negative (Haemophilus influenzae, Pseudomonas aeruginosa) and Gram-positive (Streptococcus pneumoniae) were investigated in the liquid and vapor phase...
July 17, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28713413/identification-of-conserved-and-novel-micrornas-in-blueberry
#15
Junyang Yue, Xiaohui Lu, Huan Zhang, Jiao Ge, Xueling Gao, Yongsheng Liu
MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles in cells by negatively affecting gene expression at both transcriptional and post-transcriptional levels. There have been extensive studies aiming to identify miRNAs and to elucidate their functions in various plant species. In the present study, we employed the high-throughput sequencing technology to profile miRNAs in blueberry fruits. A total of 9,992,446 small RNA tags with sizes ranged from 18 to 30 nt were obtained, indicating that blueberry fruits have a large and diverse small RNA population...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28713399/metabolite-profiling-reveals-developmental-inequalities-in-pinot-noir-berry-tissues-late-in-ripening
#16
Amanda M Vondras, Mauro Commisso, Flavia Guzzo, Laurent G Deluc
Uneven ripening in Vitis vinifera is increasingly recognized as a phenomenon of interest, with substantial implications for fruit and wine composition and quality. This study sought to determine whether variation late in ripening (∼Modified Eichhorn-Lorenz stage 39) was associated with developmental differences that were observable as fruits within a cluster initiated ripening (véraison). Four developmentally distinct ripening classes of berries were tagged at cluster véraison, sampled at three times late in ripening, and subjected to untargeted HPLC-MS to measure variation in amino acids, sugars, organic acids, and phenolic metabolites in skin, pulp, and seed tissues separately...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28713396/system-level-and-granger-network-analysis-of-integrated-proteomic-and-metabolomic-dynamics-identifies-key-points-of-grape-berry-development-at-the-interface-of-primary-and-secondary-metabolism
#17
Lei Wang, Xiaoliang Sun, Jakob Weiszmann, Wolfram Weckwerth
Grapevine is a fruit crop with worldwide economic importance. The grape berry undergoes complex biochemical changes from fruit set until ripening. This ripening process and production processes define the wine quality. Thus, a thorough understanding of berry ripening is crucial for the prediction of wine quality. For a systemic analysis of grape berry development we applied mass spectrometry based platforms to analyse the metabolome and proteome of Early Campbell at 12 stages covering major developmental phases...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28712149/occurrence-genetic-diversity-and-antibiotic-resistance-of-arcobacter-spp-in-a-dairy-plant
#18
Susana Ferreira, Mónica Oleastro, Fernanda C Domingues
AIMS: The aim of this study was to evaluate the occurrence, diversity and resistance to antibiotics of Arcobacter spp. in a dairy plant samples. METHODS AND RESULTS: A total of 75 samples from dairy plant surfaces and materials and several food products collected in different steps of the cheese production process were analysed by culture, under aerobic and microaerobic atmospheric conditions, and by enrichment molecular detection. Isolates were identified and genotyped by ERIC-PCR, and their susceptibility to nine antibiotics was evaluated by agar dilution...
July 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28711264/the-growth-and-aflatoxin-production-of-aspergillus-flavus-strains-on-a-cheese-model-system-are-influenced-by-physicochemical-factors
#19
Rocío Casquete, María José Benito, María de Guía Córdoba, Santiago Ruiz-Moyano, Alberto Martín
Traditional cheeses may be contaminated by aflatoxin-producing Aspergillus flavus during the ripening process, which has not been sufficiently taken into account. The objectives of this study were to evaluate the influence of water activity (aw), pH, and temperature on the lag phases, growth, and aflatoxin production of 3 A. flavus strains (CQ7, CQ8, and CG103) on a cheese-based medium. The results showed that the behavior of A. flavus strains was influenced by pH, aw, and temperature conditions. The CQ7 strain showed the maximum growth at pH 5...
July 12, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28711258/strains-of-the-lactobacillus-casei-group-show-diverse-abilities-for-the-production-of-flavor-compounds-in-2-model-systems
#20
Ewelina Stefanovic, Anne Thierry, Marie-Bernadette Maillard, Andrea Bertuzzi, Mary C Rea, Gerald Fitzgerald, Olivia McAuliffe, Kieran N Kilcawley
Cheese flavor development is directly connected with the metabolic activity of microorganisms used during its manufacture, and the selection of metabolically diverse strains represents a potential tool for the production of cheese with novel and distinct flavor characteristics. Strains of Lactobacillus have been proven to promote the development of important cheese flavor compounds. As cheese production and ripening are long-lasting and expensive, model systems have been developed with the purpose of rapidly screening lactic acid bacteria for their flavor potential...
July 12, 2017: Journal of Dairy Science
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