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https://www.readbyqxmd.com/read/28231288/characterization-and-expression-analysis-of-genes-encoding-ubiquitin-conjugating-domain-containing-enzymes-in-carica-papaya
#1
Dengwei Jue, Xuelian Sang, Bo Shu, Liqin Liu, Yicheng Wang, Zhiwei Jia, Yu Zou, Shengyou Shi
BACKGROUND: Ripening affects the quality and nutritional contents of fleshy fruits and is a crucial process of fruit development. Although several studies have suggested that ubiquitin-conjugating enzyme (E2s or UBC enzymes) are involved in the regulation of fruit ripening, little is known about the function of E2s in papaya (Carica papaya). METHODOLOGY/PRINCIPAL FINDINGS: In the present study, we searched the papaya genome and identified 34 putative UBC genes, which were clustered into 17 phylogenetic subgroups...
2017: PloS One
https://www.readbyqxmd.com/read/28230255/changes-in-pectin-characteristics-during-the-ripening-of-jujube-fruit
#2
Shenghua Ding, Rongrong Wang, Yang Dan, Gaoyang Li, Shiyi Ou
BACKGROUND: Pectin is relative to fruit maturation and loss of flesh firmness. In this research, changes in pectin characteristics, including pectin content, neutral sugar compositions, molecular weight (Mw ) distribution, and degree of methyl esterification (DM), in six different growth stages (S1-S6) of jujube fruit were assessed. RESULTS: The growth of jujube fruit corresponded to an increase in water-soluble pectin (WSP), and a decrease in Na2 CO3 -soluble pectin (SSP)...
February 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28226232/the-epigenome-and-transcriptional-dynamics-of-fruit-ripening
#3
James Giovannoni, Cuong Nguyen, Betsy Ampofo, Silin Zhong, Zhangjun Fei
Fruit has evolved myriad forms that facilitate seed dispersal in varied environmental and ecological contexts. Because fleshy fruits become attractive and nutritious to seed-dispersing animals, the transition from unripe to ripe fruit represents a dramatic shift in survival strategy-from protecting unripe fruit against damaging animals to making it appealing to those same animals once ripened. For optimal fitness, ripening therefore must be tightly controlled and coordinated with seed development. Fruits, like many vegetative tissues of plants that contribute to human diets, are also subject to decay, which is enhanced as a consequence of the ripening transition...
February 22, 2017: Annual Review of Plant Biology
https://www.readbyqxmd.com/read/28225260/investigation-of-ag-tio2-interfacial-reaction-of-highly-stable-ag-nanowire-transparent-conductive-film-with-conformal-tio2-coating-by-atomic-layer-deposition
#4
Ming-Hua Yeh, Po-Shen Chen, Yi-Ching Yang, Guang-Hong Chen, Hsueh-Shih Chen
The atomic layer deposition (ALD) technique is applied to coat Ag nanowires (NWs) with a highly uniform and conformal TiO2 layer to improve the stability and sustainability of Ag NW transparent conductive films (TCFs) at high temperatures. The TiO2 layer can be directly deposited on Ag NWs with a surface polyvinylpyrrolidone (PVP) coat that acts a bed for TiO2 seeding in the ALD process. The ALD TiO2 layer significantly enhances the thermal stability at least 100 folds when aged between 200-400 °C and also provides an extra function of violet-blue light filtration for Ag NW TCFs...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28224919/characteristics-and-regulatory-pathway-of-the-prupesep1-sepallata-gene-during-ripening-and-softening-in-peach-fruits
#5
Jinjin Li, Fang Li, Ming Qian, Mingyu Han, Hangkong Liu, Dong Zhang, Juanjuan Ma, Caiping Zhao
SEPALLATA genes are members of a subfamily of MADS-box transcription factors, and have essential roles in floral organ development and fruit ripening. In this study, the PrupeSEP1 gene was cloned from peach flesh. Its deduced amino acid sequence was very similar to that of MdMADS8 and MdMADS9 in apple and MADS-RIN-like in strawberry. During storage of melting flesh (MF) peach, the pattern of SEP1 expression was similar to that of ethylene biosynthesis and ethylene signal transduction-related gene expression (EIN2 and ETR2)...
April 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28224918/remodeling-of-pectin-and-hemicelluloses-in-tomato-pericarp-during-fruit-growth
#6
Fabienne Guillon, Adeline Moïse, Bernard Quemener, Brigitte Bouchet, Marie-Françoise Devaux, Camille Alvarado, Marc Lahaye
Tomato fruit texture depends on histology and cell wall architecture, both under genetic and developmental controls. If ripening related cell wall modifications have been well documented with regard to softening, little is known about cell wall construction during early fruit development. Identification of key events and their kinetics with regard to tissue architecture and cell wall development can provide new insights on early phases of texture elaboration. In this study, changes in pectin and hemicellulose chemical characteristics and location were investigated in the pericarp tissue of tomato (Solanum lycopersicon var Levovil) at four stages of development (7, 14 and 21day after anthesis (DPA) and mature green stages)...
April 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28224250/abscisic-acid-sucrose-and-auxin-coordinately-regulate-berry-ripening-process-of-the-fujiminori-grape
#7
Haifeng Jia, Zhenqiang Xie, Chen Wang, Lingfei Shangguan, Ning Qian, Mengjie Cui, Zhongjie Liu, Ting Zheng, Mengqi Wang, Jinggui Fang
The aim of this study was to examine the effect of abscisic acid (ABA), sucrose, and auxin on grape fruit development and to assess the mechanism of these three factors on the grape fruit ripening process. Different concentrations of ABA, sucrose, and auxin were used to treat the grape fruit, and the ripening-related indices, such as physiological and molecular level parameters, were analyzed. The activity of BG protein activity was analyzed during the fruit development. Sucrose, ABA, and auxin influenced the grape fruit sugar accumulation in different ways, as well as the volatile compounds, anthocyanin content, and fruit firmness...
February 21, 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/28221888/histamine-formation-in-a-dry-salted-twaite-shad-alosa-fallax-lacustris-product
#8
Mauro Vasconi, Federica Bellagamba, Cristian Bernardi, Piera Anna Martino, Vittorio Maria Moretti
Landlocked shad is a freshwater clupeid fish ( Alosa fallax lacustris ) whose consumption is associated with the risk of scombrotoxin poisoning. Traditionally, fresh shad are subjected to an artisanal processing procedure, consisting of dry salting and maturation under pressure, to give a fish product named missoltino , which is stored in large metallic barrels and is sold to local consumers and restaurants. In recent years, the introduction of modern food packaging technologies has enabled this product to also be distributed in shops and supermarkets...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221699/beehive-fences-as-a-multidimensional-conflict-mitigation-tool-for-farmers-coexisting-with-elephants
#9
Lucy E King, Fredrick Lala, Hesron Nzumu, Emmanuel Mwambingu, Iain Douglas-Hamilton
Increasing habitat fragmentation and human population growth in Africa has resulted in an escalation in human-elephant conflict between small-scale farmers and free-ranging African elephants (Loxodonta Africana). In 2012 Kenya Wildlife Service (KWS) implemented the national 10-year Conservation and Management Strategy for the Elephant in Kenya, which includes an action aimed at testing whether beehive fences can be used to mitigate human-elephant conflict. From 2012 to 2015, we field-tested the efficacy of beehive fences to protect 10 0...
February 21, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/28217387/histological-assessment-of-porous-custom-made-hydroxyapatite-implants-6-months-and-2-5-years-after-cranioplasty
#10
Hajime Ono, Taigen Sase, Yuichiro Tanaka, Hiroshi Takasuna
BACKGROUND: In cranial reconstruction, the features of artificial bone differ. Custom-made porous hydroxyapatite (HAp) implants for cranioplasty have been used all over the world because of their good cosmetic, biocompatibility, and osteoconductive properties. Surgical techniques were analyzed, and histological assessment of new bone formation in the hydroxyapatite was performed. METHODS: Over a 6-year time period, 41 patients underwent cranioplasty using a custom-made three-dimensional hybrid pore structured hydroxyapatite (3DHPoHAp) implant...
2017: Surgical Neurology International
https://www.readbyqxmd.com/read/28215984/the-cost-of-nurse-midwifery-care-use-of-interventions-resources-and-associated-costs-in-the-hospital-setting
#11
Molly R Altman, Sean M Murphy, Cynthia E Fitzgerald, H Frank Andersen, Kenn B Daratha
INTRODUCTION: Obstetrical care often involves multiple expensive, and often elective, interventions that may increase costs to patients, payers and the health care system with little effect on patient outcomes. The objectives of this study were to examine the following hospital related outcomes: 1) use of labor and birth interventions, 2) inpatient duration of stay, and 3) total direct health care costs for patients attended by a certified nurse-midwife (CNM) compared with those attended by an obstetrician-gynecologist (OB-GYN), within an environment of safe and high-quality care...
February 15, 2017: Women's Health Issues: Official Publication of the Jacobs Institute of Women's Health
https://www.readbyqxmd.com/read/28213318/efficacy-of-fungal-and-bacterial-antagonists-for-controlling-growth-fum1-gene-expression-and-fumonisin-b1-production-by-fusarium-verticillioides-on-maize-cobs-of-different-ripening-stages
#12
Nik Iskandar Putra Samsudin, Alicia Rodriguez, Angel Medina, Naresh Magan
This study was carried out to examine the efficacy of two biocontrol agents (Clonostachys rosea 016, BCA1; Gram-negative bacterium, BCA5) for control of FUM1 gene expression and fumonisin B1 (FB1) production by F. verticillioides FV1 on maize cobs of different ripening stages: R3, Milk (0.985 aw); R4, Dough (0.976 aw); R5, Dent (0.958 aw). Initially, temporal studies on FUM1 gene expression and FB1 production were performed on maize kernels for up to 14days. This revealed that day 10 was optimum for both parameters, and was used in the biocontrol studies...
February 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28213246/spinel-type-manganese-ferrite-mnfe2o4-microspheres-a-novel-affinity-probe-for-selective-and-fast-enrichment-of-phosphopeptides
#13
Xing-Yu Long, Jia-Yuan Li, Dong Sheng, Hong-Zhen Lian
The spinel-type magnetic manganese ferrite (MnFe2O4) microspheres synthesized by simple solvothermal method were used as a novel adsorbent for selective enrichment and effective isolation of phosphopeptides. The uniform MnFe2O4 magnetic affinity microspheres (MAMSs) had a narrow particle size distribution between 250 and 260nm, and displayed superparamagnetism with a saturation magnetization value of 67.0emu/g. Comprehensively, the possible formation mechanism of MnFe2O4 microspheres with ferric and manganous sources as dual precursors was elucidated by comparison with those of Fe3O4 nanoparticles and MnOOH nanosheets respectively with either ferric or manganous source as single precursor...
May 1, 2017: Talanta
https://www.readbyqxmd.com/read/28204882/comparison-between-vaginal-and-sublingual-misoprostol-50%C3%A2-%C3%A2%C2%B5g-for-cervical-ripening-prior-to-induction-of-labor-randomized-clinical-trial
#14
Andrés Conde, Sebastián Ben, Josefina Tarigo, Santiago Artucio, Virginia Varela, Pamela Grimaldi, Claudio Sosa, Justo Alonso
OBJECTIVE: To compare the effectiveness and safety of sublingual versus vaginal misoprostol on improving the Bishop score after 6 h of administration. METHODS: Randomized clinical trial which includes pregnant women in gestational ages from 32/0 to 41/6, with indication of induction of labor with misoprostol. Bishop score was assessed at the time of induction and 6 h after administration of 50 µg misoprostol. Analysis was made over difference in mean Bishop score of 2 points, using a standard deviation of 2, with 90% power, reaching a 95% confidence interval...
February 16, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28203646/light-exclusion-influence-on-grape-anthocyanin
#15
Jungmin Lee
This study contrasted the anthocyanins of investigational grape clusters that developed without light incidence (light-excluded), to those of control clusters that were shaded naturally beneath the vine canopy (control-shaded). Treatment grape clusters were light-excluded during ripening by opaque white polypropylene enclosures; temperature, vapor pressure deficit, and light intensity were measured continually. All 15 'Merlot' grape anthocyanins accrued in both groups, indicating no accumulations were terminated from light-exclusion during ripening...
February 2017: Heliyon
https://www.readbyqxmd.com/read/28197155/dissecting-the-biochemical-and-transcriptomic-effects-of-a-locally-applied-heat-treatment-on-developing-cabernet-sauvignon-grape-berries
#16
Fatma Lecourieux, Christian Kappel, Philippe Pieri, Justine Charon, Jérémy Pillet, Ghislaine Hilbert, Christel Renaud, Eric Gomès, Serge Delrot, David Lecourieux
Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196350/overexpression-of-the-carbohydrate-binding-module-from-solanum-lycopersicum-expansin-1-sl-exp1-modifies-tomato-fruit-firmness-and-botrytis-cinerea-susceptibility
#17
M A Perini, I N Sin, N M Villarreal, M Marina, A L T Powell, G A Martínez, P M Civello
Firmness, one of the major determinants of postharvest quality and shelf life of fruits is determined by the mechanical resistance imposed by the plant cell wall. Expansins (EXP) are involved in the non-hydrolytic metabolic disassembly of plant cell walls, particularly in processes where relaxation of the wall is necessary, such as fruit development and ripening. As many carbohydrate-associated proteins, expansins have a putative catalytic domain and a carbohydrate-binding module (CBM). Several strategies have been pursued to control the loss of fruit firmness during storage...
February 2, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28195221/transcriptome-analysis-around-the-onset-of-strawberry-fruit-ripening-uncovers-an-important-role-of-oxidative-phosphorylation-in-ripening
#18
Qing-Hua Wang, Cheng Zhao, Miao Zhang, Yu-Zhong Li, Yuan-Yue Shen, Jia-Xuan Guo
Although much progress has been made towards understanding the ripening of non-climacteric fruit using the strawberry as a model plant, the defined molecular mechanisms remain unclear. Here, RNA-sequencing was performed using four cDNA libraries around the onset of ripening, and a total of 31,793 unigenes and 335 pathways were annotated including the top five pathways, which were involved in ribosome, spliceosome, protein processing, plant-pathogen interaction and plant hormone signaling, and the important DEGs related to ripening were annotated to be mainly involved in protein translation and processing, sugar metabolism, energy metabolism, phytohormones, antioxidation, pigment and softening, especially finding a decreased trend of oxidative phosphorylation during red-coloring...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28192158/relating-emulsion-stability-to-interfacial-properties-for-pharmaceutical-emulsions-stabilized-by-pluronic-f68-surfactant
#19
Kristin Conrad Powell, Robert Damitz, Anuj Chauhan
We explore mechanisms of emulsion stability for several systems using Pluronic F68 and a range of oils commonly used in pharmaceutics and cosmetics. We report measurements of dynamic emulsion drop size, zeta potential, and creaming time, as well as dynamic interfacial tension and interfacial viscoelasticity. Experiments show that with 1wt% Pluronic F68, soybean oil emulsions were the most stable with no creaming over six months, followed by isopropyl myristate, octanoic acid, and then ethyl butyrate. The eventual destabilization occurred due to the rising of large drops which formed through Ostwald ripening and coalescence...
February 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28190337/observed-dependence-of-colloid-detachment-on-the-concentration-of-initially-attached-colloids-and-collector-surface-heterogeneity-in-porous-media
#20
Tiantian Li, Yan Jin, Yuanfang Huang, Baoguo Li, Chongyang Shen
Sand column experiments were conducted to examine the effects of the concentration of attached colloids (CAC) on their subsequent detachment upon decreasing solution ionic strength (IS). Different pore volumes of latex microparticle suspensions were injected into the columns to allow different amounts of colloids to attach at ISs of 0.001, 0.01, and 0.2 M. Then, deionized water was introduced to release the attached colloids. Results show that the fraction of attachments that were reversible to reduction of IS (FRA) increased with increasing CAC at a given IS if the sand was extensively treated using acids to reduce surface charge heterogeneity...
February 23, 2017: Environmental Science & Technology
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