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Chondrocyte transplant

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https://www.readbyqxmd.com/read/28105130/biological-characterization-of-sheep-kidney-derived-mesenchymal-stem-cells
#1
Meng Ji, Chunyu Bai, Lu Li, Ya'Nan Fan, Caiyun Ma, Xiangchen Li, Weijun Guan
The aim of the present study was to isolate, culture and characterize sheep metanephric mesenchymal stem cells (MMSCs). The MMSCs were isolated from the kidney tissue of six-week-old sheep fetus. This study investigated whether primary MMSCs could be grown for 26 passages and expressed Oct-4, which is involved in the self-renewal of undifferentiated pluripotent stem cells. The MMSCs also expressed the renal lineage marker gene PAX2, and mesenchymal cell marker genes CD44, FN1 and VIM. Expression of these genes was detected using immunofluorescence and reverse transcription-polymerase chain reaction assays...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28045868/failure-of-autologous-chondrocyte-implantation
#2
Luca Andriolo, Giulia Merli, Giuseppe Filardo, Maurilio Marcacci, Elizaveta Kon
: Long-term results of autologous chondrocyte implantation and matrix-assisted autologous chondrocyte transplantation in the knee are satisfying, but not enough attention has been paid to the evaluation of failures. Thus, a systematic review of the literature was performed, underlining a failure rate in the 58 included articles of 14.9% among 4294 patients, most of them occurring in the first 5 years after surgery, and with no difference between autologous chondrocyte implantation and matrix-assisted autologous chondrocyte transplantation...
March 2017: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/28024231/fabrication-of-injectable-high-strength-hydrogel-based-on-4-arm-star-peg-for-cartilage-tissue-engineering
#3
Jianqi Wang, Fengjie Zhang, Wing Pui Tsang, Chao Wan, Chi Wu
Hydrogels prepared from poly(ethylene glycol) (PEG) are widely applied in tissue engineering, especially those derived from a combination of functional multi-arm star PEG and linear crosslinker, with an expectation to form a structurally ideal network. However, the poor mechanical strength still renders their further applications. Here we examined the relationship between the dynamics of the pre-gel solution and the mechanical property of the resultant hydrogel in a system consisting of 4-arm star PEG functionalized with vinyl sulfone and short dithiol crosslinker...
December 20, 2016: Biomaterials
https://www.readbyqxmd.com/read/28019703/a-clinically-relevant-hydrogel-based-on-hyaluronic-acid-and-platelet-rich-plasma-as-a-carrier-for-mesenchymal-stem-cells-rheological-and-biological-characterization
#4
Gianluca Vadalà, Fabrizio Russo, Maria Musumeci, Matteo D'Este, Caterina Cattani, Giuseppina Catanzaro, Maria Cristina Tirindelli, Lorenza Lazzari, Mauro Alini, Rosaria Giordano, Vincenzo Denaro
Intervertebral disc regeneration is quickly moving towards clinical applications. However, it is still missing an ideal injectable hydrogel to support mesenchymal stem cells (MSC) delivery. Herein, a new injectable hydrogel composed of platelet rich plasma (PRP) and hyaluronic acid (HA) blended with batroxobin (BTX) as gelling agent, was designed to generate a clinically relevant cell carrier for disc regeneration. PRP/HA/BTX blend was tested for rheological properties. Amplitude sweep, frequency sweep and rotational measurements were performed and viscoelastic properties were evaluated...
December 26, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28011039/the-direction-of-human-mesenchymal-stem-cells-into-the-chondrogenic-lineage-is-influenced-by-the-features-of-hydrogel-carriers
#5
A Hansson, A Wenger, H Barreto Henriksson, S Li, B R Johansson, H Brisby
Low back pain is a major public health issue in the Western world, one main cause is believed to be intervertebral disc (IVD) degeneration. To halt/diminish IVD degeneration, cell therapy using different biomaterials e.g. hydrogels as cell carriers has been suggested. In this study, two different hydrogels were examined (in vitro) as potential cell carriers for human mesenchymal stem cells (hMSCs) intended for IVD transplantation. The aim was to investigate cell-survival and chondrogenic differentiation of hMSCs when cultured in hydrogels Puramatrix(®) or Hydromatrix(®) and potential effects of stimulation with growth hormone (GH)...
December 13, 2016: Tissue & Cell
https://www.readbyqxmd.com/read/27994717/evaluation-and-management-of-osteochondral-lesions-of-the-talus
#6
REVIEW
Christopher A Looze, Jason Capo, Michael K Ryan, John P Begly, Cary Chapman, David Swanson, Brian C Singh, Eric J Strauss
Osteochondral lesions of the talus are common injuries that affect a wide variety of active patients. The majority of these lesions are associated with ankle sprains and fractures though several nontraumatic etiologies have also been recognized. Patients normally present with a history of prior ankle injury and/or instability. In addition to standard ankle radiographs, magnetic resonance imaging and computed tomography are used to characterize the extent of the lesion and involvement of the subchondral bone...
January 2017: Cartilage
https://www.readbyqxmd.com/read/27925229/plga-ptmc-cultured-bone-mesenchymal-stem-cell-scaffold-enhances-cartilage-regeneration-in-tissue-engineered-tracheal-transplantation
#7
Bingyang Yan, Zhipei Zhang, Xiaoping Wang, Yunfeng Ni, Yongshi Liu, Tao Liu, Wuping Wang, Hao Xing, Ying Sun, Jian Wang, Xiao-Fei Li
The treatment of long-segment tracheal defect requires the transplantation of effective tracheal substitute, and the tissue-engineered trachea (TET) has been proposed as an ideal tracheal substitute. The major cause of the failure of segmental tracheal defect reconstruction by TET is airway collapse caused by the chondromalacia of TET cartilage. The key to maintain the TET structure is the regeneration of chondrocytes in cartilage, which can secrete plenty of cartilage matrices. To address the problem of the chondromalacia of TET cartilage, this study proposed an improved strategy...
December 7, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27919783/the-potential-of-induced-pluripotent-stem-cells-as-a-tool-to-study-skeletal-dysplasias-and-cartilage-related-pathologic-conditions
#8
REVIEW
H Liu, L Yang, F F Yu, S Wang, C Wu, C Qu, M J Lammi, X Guo
The development of induced pluripotent stem cells (iPSCs) technology has opened up new horizons for development of new research tools especially for skeletal dysplasias, which often lack human disease models. Regenerative medicine and tissue engineering could be the next areas to benefit from refinement of iPSC methods to repair focal cartilage defects, while applications for osteoarthritis (OA) and drug screening have evolved rather slowly. Although the advances in iPSC research of skeletal dysplasias and repair of focal cartilage lesions are not directly relevant to OA, they can be considered to pave the way to future prospects and solutions to OA research, too...
December 3, 2016: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/27919119/-osteochondritis-dissecans-of-the-talus-a-critical-review
#9
J Bruns, C R Habermann, J P Petersen
The etiology remains unclear. Mechanical factors seem to play an important role. Most histologies report clefts between the cartilage and bone and necrotic areas. Several classifications have been published based on observations from imaging techniques or on intraoperative findings. Clinical symptoms are unspecific. Imaging techniques are of great importance: X-rays of the ankle joint enable a quick diagnostic overview. MRT is the imaging-technique of choice for diagnosing OCDT-lesions. Regarding treatment, a tremendous number of retrospective publications exist...
April 2016: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/27846904/an-ex-vivo-human-cartilage-repair-model-to-evaluate-the-potency-of-a-cartilage-cell-transplant
#10
Christoph Bartz, Miriam Meixner, Petra Giesemann, Giulietta Roël, Grit-Carsta Bulwin, Jeske J Smink
BACKGROUND: Cell-based therapies such as autologous chondrocyte implantation are promising therapeutic approaches to treat cartilage defects to prevent further cartilage degeneration. To assure consistent quality of cell-based therapeutics, it is important to be able to predict the biological activity of such products. This requires the development of a potency assay, which assesses a characteristic of the cell transplant before implantation that can predict its cartilage regeneration capacity after implantation...
November 15, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27829414/repair-of-cartilage-defects-in-osteoarthritis-rats-with-induced-pluripotent-stem-cell-derived-chondrocytes
#11
Yanxia Zhu, Xiaomin Wu, Yuhong Liang, Hongsheng Gu, Kedong Song, Xuenong Zou, Guangqian Zhou
BACKGROUND: The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA) and induced pluripotent stem cells (iPSCs) are becoming a promising stem cell source. RESULTS: Three steps were developed to differentiate human iPSCs into chondrocytes which were transplanted into rat OA models induced by monosodium iodoacetate (MIA)...
November 9, 2016: BMC Biotechnology
https://www.readbyqxmd.com/read/27803938/biological-knee-reconstruction-with-concomitant-autologous-chondrocyte-implantation-and-meniscal-allograft-transplantation-mid-to-long-term-outcomes
#12
Takahiro Ogura, Tim Bryant, Tom Minas
BACKGROUND: Treating articular cartilage defects and meniscal deficiency is challenging. Although some short- to mid-term follow-up studies report good clinical outcomes after concurrent autologous chondrocyte implantation (ACI) and meniscal allograft transplantation (MAT), longer follow-up is needed. PURPOSE: To evaluate mid- to long-term outcomes after combined ACI with MAT. STUDY DESIGN: Case series; Level of evidence, 4. METHODS: We performed a retrospective review of prospectively gathered data from patients who had undergone ACI with MAT between 1999 and 2013...
October 2016: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/27770869/a-new-bi-layered-scaffold-for-osteochondral-tissue-regeneration-in-vitro-and-in-vivo-preclinical-investigations
#13
M Sartori, S Pagani, A Ferrari, V Costa, V Carina, E Figallo, M C Maltarello, L Martini, M Fini, G Giavaresi
Current treatments for acute or degenerative chondral and osteochondral lesions are in need of improvement, as these types of injuries lead to disability and worsen the quality of life in a high percentage of patients. The aim of this study was to develop a new bi-layered scaffold for osteochondral tissue regeneration through a "biomimetic" and "bioinspired" approach. For chondral regeneration, the scaffold was realized with an organic compound (type I collagen), while for the regeneration of the subchondral layer, bioactive magnesium-doped hydroxyapatite (Mg/HA) crystals were co-precipitated with the organic component of the scaffold...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27770634/repair-of-bone-defects-in%C3%A2-vivo-using-tissue-engineered-hypertrophic-cartilage-grafts-produced-from-nasal-chondrocytes
#14
Katie Bardsley, Agnieska Kwarciak, Christine Freeman, Ian Brook, Paul Hatton, Aileen Crawford
The regeneration of large bone defects remains clinically challenging. The aim of our study was to use a rat model to use nasal chondrocytes to engineer a hypertrophic cartilage tissue which could be remodelled into bone in vivo by endochondral ossification. Primary adult rat nasal chondrocytes were isolated from the nasal septum, the cell numbers expanded in monolayer culture and the cells cultured in vitro on polyglycolic acid scaffolds in chondrogenic medium for culture periods of 5-10 weeks. Hypertrophic differentiation was assessed by determining the temporal expression of key marker genes and proteins involved in hypertrophic cartilage formation...
October 11, 2016: Biomaterials
https://www.readbyqxmd.com/read/27769090/-treatment-of-osteochondral-lesions-in-the-ankle-a-guideline-from-the-group-clinical-tissue-regeneration-of-the-german-society-of-orthopaedics-and-traumatology-dgou
#15
M Aurich, D Albrecht, P Angele, C Becher, S Fickert, J Fritz, P E Müller, P Niemeyer, M Pietschmann, G Spahn, M Walther
Background: Osteochondral lesions (OCL) of the ankle are a common cause of ankle pain. Although the precise pathophysiology has not been fully elucidated, it can be assumed that a variety of factors are responsible, mainly including traumatic events such as ankle sprains. Advances in arthroscopy and imaging techniques, in particular magnetic resonance imaging (MRI), have improved the possibilities for the diagnosis of OCLs of the ankle. Moreover, these technologies aim at developing new classification systems and modern treatment strategies...
October 21, 2016: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/27762664/limited-immunogenicity-of-human-induced-pluripotent-stem-cell-derived-cartilages
#16
Takeshi Kimura, Akihiro Yamashita, Keiichi Ozono, Noriyuki Tsumaki
Articular cartilage damage does not spontaneously heal and could ultimately result in a loss of joint function. Damaged cartilage can be repaired with cell/tissue sources that are transplanted, however, autologous chondrocytes are limited in number as a cell source. Induced pluripotent stem cells (iPSCs) are a relatively new and abundant cell source and can be made from the patient, but at a considerable cost. Because cartilage is immunoprivileged tissue, allogeneic cartilages have been transplanted effectively without matching for human leukocyte antigen (HLA), but are difficult to acquire due to scarcity of donors...
December 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27735950/increased-survival-and-cell-cycle-progression-pathways-are-required-for-ews-fli1-induced-malignant-transformation
#17
Tahereh Javaheri, Zahra Kazemi, Jan Pencik, Ha Tt Pham, Maximilian Kauer, Rahil Noorizadeh, Barbara Sax, Harini Nivarthi, Michaela Schlederer, Barbara Maurer, Maximillian Hofbauer, Dave Nt Aryee, Marc Wiedner, Eleni M Tomazou, Malcolm Logan, Christine Hartmann, Jan P Tuckermann, Lukas Kenner, Mario Mikula, Helmut Dolznig, Aykut Üren, Günther H Richter, Florian Grebien, Heinrich Kovar, Richard Moriggl
Ewing sarcoma (ES) is the second most frequent childhood bone cancer driven by the EWS/FLI1 (EF) fusion protein. Genetically defined ES models are needed to understand how EF expression changes bone precursor cell differentiation, how ES arises and through which mechanisms of inhibition it can be targeted. We used mesenchymal Prx1-directed conditional EF expression in mice to study bone development and to establish a reliable sarcoma model. EF expression arrested early chondrocyte and osteoblast differentiation due to changed signaling pathways such as hedgehog, WNT or growth factor signaling...
October 13, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27718100/erratum-to-a-prospective-randomized-multicenter-phase-i-ii-clinical-trial-to-evaluate-safety-and-efficacy-of-novocart-disk-plus-autologous-disk-chondrocyte-transplantation-in-the-treatment-of-nucleotomized-and-degenerative-lumbar-disks-to-avoid-secondary-disease
#18
Anja Tschugg, Michael Diepers, Simone Steinert, Felix Michnacs, Sebastian Quirbach, Martin Strowitzki, Hans Jörg Meisel, Claudius Thomé
No abstract text is available yet for this article.
January 2017: Neurosurgical Review
https://www.readbyqxmd.com/read/27717559/peritoneum-and-omentum-are-natural-reservoirs-for-chondrocytes-of%C3%A2-osteochondral-autografts-a-comparative-animal-study
#19
Kadir Buyukdogan, Mahmut Nedim Doral, Onur Bilge, Egemen Turhan, Gazi Huri, Mustafa Fevzi Sargon
OBJECTIVE: The purpose of this study was to investigate the effects of the omentum, peritoneum, paratenon and skeletal muscle on the proliferation of the cartilage tissue using rabbit model as an in vivo culture medium. METHODS: 6 months old forty-five New Zealand rabbits were randomized into omentum, peritoneum, muscle, and Achilles paratenon groups. Standard sized osteochondral grafts were harvested from right knees and immediately placed into the specified tissues...
October 2016: Acta Orthopaedica et Traumatologica Turcica
https://www.readbyqxmd.com/read/27696973/tracheal-tissue-engineering-in-vivo-biocompatibility-of-mechanically-stripped-allogenic-rabbit-trachea-with-autologous-epithelial-covering
#20
Margot Den Hondt, Bart M Vanaudenaerde, Erik K Verbeken, Jan J Vranckx
BACKGROUND: Successful trachea transplantation comprises the use of biocompatible constructs with little immune-reactivity, submucosal revascularization and creation of an epithelial covering. Allogenic chondrocytes might be protected from an overt immune-response due to physical isolation. Our aim was to evaluate in-vivo biocompatibility of allotracheae, stripped of their highly-immunogenic inner lining. Secondly, we established whether these constructs might serve as suitable scaffolds for autologous epithelial grafting...
October 4, 2016: Acta Chirurgica Belgica
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