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Chondrocyte transplant

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https://www.readbyqxmd.com/read/29913540/-significance-of-matrix-augmented-bone-marrow-stimulation-for-treatment-of-cartilage-defects-of-the-knee-a-consensus-statement-of-the-dgou-working-group-on-tissue-regeneration
#1
Philipp Niemeyer, Christoph Becher, Matthias Buhs, Stefan Fickert, Kolja Gelse, Daniel Günther, Raphael Kaelin, Peter Kreuz, Jörg Lützner, Stefan Nehrer, Henning Madry, Stefan Marlovits, Julian Mehl, Henning Ott, Matthias Pietschmann, Gunther Spahn, Thomas Tischer, Martin Volz, Markus Walther, Götz Welsch, Johannes Zellner, Wolfgang Zinser, Peter Angele
Surgical principles for treatment of full-thickness cartilage defects of the knee include bone marrow stimulation techniques (i.e. arthroscopic microfracturing) and transplantation techniques (i.e. autologous chondrocyte implantation and osteochondral transplantation). On the basis of increasing scientific evidence, indications for these established therapeutical concepts have been specified and clear recommendations for practical use have been given. Within recent years, matrix-augmented bone marrow stimulation has been established as a new treatment concept for chondral lesions...
June 18, 2018: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29908335/platelet-derived-growth-factor-coated-decellularized-meniscus-scaffold-for-integrative-healing-of-meniscus-tears
#2
Kwang Il Lee, Merissa Olmer, Jihye Baek, Darryl D D'Lima, Martin K Lotz
The aim of this study was to examine the potential of platelet-derived growth factor (PDGF)-coated decellularized meniscus scaffold in mediating integrative healing of meniscus tears by inducing endogenous cell migration. Fresh bovine meniscus was chemically decellularized and covalently conjugated with heparin and PDGF-BB. In vitro PDGF release kinetics was measured. The scaffold was transplanted into experimental tears in avascular bovine meniscus explants and cultured for 2 and 4 weeks. The number migrating and proliferating cells at the borderline between the scaffold and injured explant and PDGF receptor-β (PDGFR β) expressing cells were counted...
June 13, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29907992/surface-chemistry-substrate-and-topography-guide-the-behavior-of-human-articular-chondrocytes-cultured-in-vitro
#3
Natasja Leth Bergholt, Morten Foss, Anwer Saeed, Nikolaj Gadegaard, Helle Lysdahl, Martin Lind, Casper Bindzus Foldager
Understanding the behavior of chondrocytes in contact with artificial culture surfaces is becoming increasingly important in attaining appropriate ex vivo culture conditions of chondrocytes in cartilage regeneration. Chondrocyte transplantation-based cartilage repair requires efficiently expanded chondrocytes and the culture surface plays an important role in guiding the behavior of the cell. Micro- and nanoengineered surfaces make it possible to modulate cell behavior. We hypothesized that the combined influence of topography, substrate, and surface chemistry may affect the chondrocyte culturing in terms of proliferation and phenotypic means...
June 16, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29876883/tsmu-solution-improves-rabbit-osteochondral-allograft-preservation-and-transplantation-outcome
#4
Famin Cao, Jianhong Qi, Hongqiang Song, Di Xie, Lu Zhou, Yunning Han, Hao Li, Qi Wu, Jun Dong, Yanming Zhang
To compare the effects of Tsmu solution with vitrification on chondrocyte viability and examine histological and biomechanical properties of osteochondral allografts (OCAs) after storage, OCAs from femoral condyles of New Zealand rabbits were harvested, stored for 35 days in Tsmu solution or by in vitro vitrification, and subjected to in vivo and in vitro assays. Stored OCAs were transplanted into knee femoral condyle cartilage defects in recipient rabbits. Chondrocyte viability and histological changes of cartilage grafts were assessed in vitro...
June 6, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29875586/human-umbilical-cord-derived-mesenchymal-stem-cells-reduce-monosodium-iodoacetate-induced-apoptosis-in-cartilage
#5
Yu-Hsun Chang, Kun-Chi Wu, Hwan-Wun Liu, Tang-Yuan Chu, Dah-Ching Ding
Objective: The present study investigated the therapeutic potential and underlying mechanisms of human umbilical cord mesenchymal stem cells (HUCMSCs) on joint cartilage destruction induced by monosodium iodoacetate (MIA) in mice. Materials and Methods: HUCMSCs were tested for mesenchymal stem cell (MSC) characteristics including surface markers by flow cytometry and mesoderm differentiation (adipogenesis, osteogenesis, and chondrogenesis). Terminal deoxynucleotidyl transferase dUTP nick end labeling assay and Western blot assay were used to evaluate MIA-induced chondrocyte apoptosis...
April 2018: Ci Ji Yi Xue za Zhi, Tzu-chi Medical Journal
https://www.readbyqxmd.com/read/29848826/-cartilage-chondrocyte-research-and-osteoarthritis-regeneration-of-articular-cartilage-damage-using-ips-cell-derived-cartilage
#6
Noriyuki Tsumaki
Induced pluripotent stem (iPS)cells have capacities of self-renewal and pluripotency. We have developed a method to differentiate human iPS cells toward chondrocytes, followed by the creation of cartilage tissue composed of chondrocytes and cartilage extracellular matrix. The mechanism through which tissue transplantation repairs cartilage defects involves the transplant itself constituting the repair tissue. Human iPS cell-derived cartilage has low immunogenicity and can be transplanted in an allogeneic manner...
2018: Clinical Calcium
https://www.readbyqxmd.com/read/29806277/-construction-of-tissue-engineered-cartilage-based-on-acellular-cartilage-extracellular-matrix-oriented-scaffold-and-chondrocytes
#7
Liang Lu, Bin Liu, Xifu Shang, Yu Zhang, Weijian Chen, Shuyun Liu, Jingxiang Huang, Aiyuan Wang, Quanyi Guo, Shibi Lu
Objective: To observe the feasibility of acellular cartilage extracellular matrix (ACECM) oriented scaffold combined with chondrocytes to construct tissue engineered cartilage. Methods: Chondrocytes from the healthy articular cartilage tissue of pig were isolated, cultured, and passaged. The 3rd passage chondrocytes were labeled by PKH26. After MTT demonstrated that PKH26 had no influence on the biological activity of chondrocytes, labeled and unlabeled chondrocytes were seeded on ACECM oriented scaffold and cultivated...
March 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29777422/patellofemoral-cartilage-repair
#8
REVIEW
Alexandre Barbieri Mestriner, Jakob Ackermann, Andreas H Gomoll
PURPOSE OF REVIEW: This review provides an overview of well-established and newly developed cartilage repair techniques for cartilage defects in the patellofemoral joint (PFJ). An algorithm will be presented for approaching cartilage defects considering the distinct anatomy of both the patellar and trochlear articular surfaces. RECENT FINDINGS: Recent studies on cartilage repair in the PFJ have demonstrated improved outcomes in an attempt to delay or obviate the need for arthroplasty, and improve symptoms in young patients...
June 2018: Current Reviews in Musculoskeletal Medicine
https://www.readbyqxmd.com/read/29770595/repair-potential-of-non-surgically-delivered-induced-pluripotent-stem-cell-derived-chondrocytes-in-a-rat-osteochondral-defect-model
#9
Yeri Alice Rim, Yoojun Nam, Narae Park, Jennifer Lee, Sung-Hwan Park, Ji Hyeon Ju
Human induced pluripotent stem cells (hiPSCs) are thought to be an alternative cell source for future regenerative medicine. hiPSCs may allow unlimited production of cell types that have low turnover rates and are difficult to obtain such as autologous chondrocytes. In this study, we generated hiPSC-derived chondrogenic pellets and chondrocytes were isolated. To confirm the curative effects, chondrogenic pellets and isolated chondrocytes were transplanted into rat joints with osteochondral defects. Isolated hiPSC-derived chondrocytes were delivered in the defect by a single intra-articular injection...
May 16, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29765410/comparison-of-regenerative-tissue-quality-following-matrix-associated-cell-implantation-using-amplified-chondrocytes-compared-to-synovium-derived-stem-cells-in-a-rabbit-model-for-cartilage-lesions
#10
Hagen Schmal, Justyna M Kowal, Moustapha Kassem, Michael Seidenstuecker, Anke Bernstein, Katharina Böttiger, Tanshiyue Xiong, Norbert P Südkamp, Eva J Kubosch
Known problems of the autologous chondrocyte implantation motivate the search for cellular alternatives. The aim of the study was to test the potential of synovium-derived stem cells (SMSC) to regenerate cartilage using a matrix-associated implantation. In an osteochondral defect model of the medial femoral condyle in a rabbit, a collagen membrane was seeded with either culture-expanded allogenic chondrocytes or SMSC and then transplanted into the lesion. A tailored piece synovium served as a control. Rabbit SMSC formed typical cartilage in vitro...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29731871/co-implantation-of-bone-marrow-mesenchymal-stem-cells-and-chondrocytes-increase-the-viability-of-chondrocytes-in-rat-osteo-chondral-defects
#11
Zhi Zhao, Xinshe Zhou, Jianzhong Guan, Min Wu, Jiansheng Zhou
Replacement of chondrocytes by adult stem cells was believed to improve the performance of autologous chondrocytes transplantation, since less chondrocytes were needed. Previous studies have demonstrated that the increased cartilage production in pellet co-cultures of chondrocytes and bone marrow mesenchymal stem cells (BMSCs) is due to the trophic effects of the MSC by stimulating chondrocyte proliferation and matrix production. However, the destination of MSCs or chondrocytes after implanted in osteo-chondral defects is not clear...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29699841/lateral-meniscus-allograft-transplantation-with-platelet-rich-plasma-injections-a-minimum-two-year-follow-up-study
#12
Hua Zhang, Shiyang Chen, Man Qiu, Aiguo Zhou, Wenlong Yan, Jian Zhang
BACKGROUND: The aim of this study was to report the short-term clinical and imaging outcomes of lateral meniscus allograft transplantations (LMAT) combined with intra-articular platelet-rich plasma (PRP) injection. METHODS: Thirty-three patients who had undergone LMAT combined with intra-articular PRP injection were evaluated. The Lysholm, International Knee Documentation Committee (IKDC), Western Ontario and McMaster Universities Osteoarthritis Index, Tegner activity level scale and visual analog scale for pain scores were used to evaluate the outcomes...
April 24, 2018: Knee
https://www.readbyqxmd.com/read/29697499/management-of-articular-cartilage-defects-in-the-glenohumeral-joint
#13
Adam J Seidl, Matthew J Kraeutler
Articular cartilage defects are not often encountered in the glenohumeral joint. These lesions are typically found in patients with shoulder trauma, recurrent instability, or previous surgical treatment. Diagnosis can be difficult; these defects are often found incidentally during arthroscopic or open surgical management of other pathology. Initial management of isolated glenohumeral chondral defects is nonsurgical and includes physical therapy and/or corticosteroid injections. If nonsurgical treatment is unsuccessful, patients may undergo surgery...
June 1, 2018: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/29695308/the-presence-of-human-mesenchymal-stem-cells-of-renal-origin-in-amniotic-fluid-increases-with-gestational-time
#14
Md Shaifur Rahman, Lucas-Sebastian Spitzhorn, Wasco Wruck, Carsten Hagenbeck, Percy Balan, Nina Graffmann, Martina Bohndorf, Audrey Ncube, Pascale V Guillot, Tanja Fehm, James Adjaye
BACKGROUND: Established therapies for managing kidney dysfunction such as kidney dialysis and transplantation are limited due to the shortage of compatible donated organs and high costs. Stem cell-based therapies are currently under investigation as an alternative treatment option. As amniotic fluid is composed of fetal urine harboring mesenchymal stem cells (AF-MSCs), we hypothesized that third-trimester amniotic fluid could be a novel source of renal progenitor and differentiated cells...
April 25, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29693628/influence-of-mechanical-unloading-on-articular-chondrocyte-dedifferentiation
#15
Simon L Wuest, Martina Caliò, Timon Wernas, Samuel Tanner, Christina Giger-Lange, Fabienne Wyss, Fabian Ille, Benjamin Gantenbein, Marcel Egli
Due to the limited self-repair capacity of articular cartilage, the surgical restoration of defective cartilage remains a major clinical challenge. The cell-based approach, which is known as autologous chondrocyte transplantation (ACT), has limited success, presumably because the chondrocytes acquire a fibroblast-like phenotype in monolayer culture. This unwanted dedifferentiation process is typically addressed by using three-dimensional scaffolds, pellet culture, and/or the application of exogenous factors...
April 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29691830/stem-cells-in-osteochondral-tissue-engineering
#16
Eleonora Pintus, Matteo Baldassarri, Luca Perazzo, Simone Natali, Diego Ghinelli, Roberto Buda
Mesenchymal stem cells (MSCs) are pluripotent stem cells with the ability to differentiate into a variety of other connective tissue cells, such as chondral, bony, muscular, and tendon tissue. Bone marrow-derived MSCs are pluripotent cells that can differentiate among others into osteoblasts, adipocytes and chondrocytes.Bone marrow-derived cells may represent the future in osteochondral repair. A one-step arthroscopic technique is developed for cartilage repair, using a device to concentrate bone marrow-derived cells and collagen powder or hyaluronic acid membrane as scaffolds for cell support and platelet gel...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29625584/temporomandibular-joint-regeneration-proposal-of-a-novel-treatment-for-condylar-resorption-after-orthognathic-surgery-using-transplantation-of-autologous-nasal-septum-chondrocytes-and-the-first-human-case-report
#17
Ricardo de Souza Tesch, Esther Rieko Takamori, Karla Menezes, Rosana Bizon Vieira Carias, Cláudio Leonardo Milione Dutra, Marcelo de Freitas Aguiar, Tânia Salgado de Sousa Torraca, Alexandra Cristina Senegaglia, Cármen Lúcia Kuniyoshi Rebelatto, Debora Regina Daga, Paulo Roberto Slud Brofman, Radovan Borojevic
BACKGROUND: Upon orthognathic mandibular advancement surgery the adjacent soft tissues can displace the distal bone segment and increase the load on the temporomandibular joint causing loss of its integrity. Remodeling of the condyle and temporal fossa with destruction of condylar cartilage and subchondral bone leads to postsurgical condylar resorption, with arthralgia and functional limitations. Patients with severe lesions are refractory to conservative treatments, leading to more invasive therapies that range from simple arthrocentesis to open surgery and prosthesis...
April 7, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29621359/applying-chlorogenic-acid-in-an-alginate-scaffold-of-chondrocytes-can-improve-the-repair-of-damaged-articular-cartilage
#18
Xin Cheng, Ke Li, Shengsong Xu, Peizhi Li, Yu Yan, Guang Wang, Zachary Berman, Rui Guo, Jianxin Liang, Sira Traore, Xuesong Yang
Damaged cartilage has very low regenerative potential which has led to the search for novel tissue-engineering approaches to help treat cartilage defects. While various approaches have been reported, there is no perfect treatment currently. In this study we evaluated the effects of a plant extract, chlorogenic acid (CGA), as part of chondrocyte transplantation on a model of knee joint injury in chicks. First, primary cultured chondrocytes used to evaluate the effects of CGA on chondrogenesis. Then using an articular cartilage injury model of chick knee we assessed the functional recovery after transplantation of the complexes containing chondrocytes and CGA in an alginate scaffold...
2018: PloS One
https://www.readbyqxmd.com/read/29619397/treatment-of-failed-articular-cartilage-reconstructive-procedures-of-the-knee-a-systematic-review
#19
REVIEW
Joseph D Lamplot, Kevin A Schafer, Matthew J Matava
Background: Symptomatic articular cartilage lesions of the knee are common and are being treated surgically with increasing frequency. While many studies have reported outcomes following a variety of cartilage restoration procedures, few have investigated outcomes of revision surgery after a failed attempt at cartilage repair or reconstruction. Purpose: To investigate outcomes of revision cartilage restoration procedures for symptomatic articular cartilage lesions of the knee following a previously failed cartilage reconstructive procedure...
March 2018: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/29615915/application-of-mesenchymal-stem-cells-for-therapeutic-agent-delivery-in-anti-tumor-treatment
#20
REVIEW
Daria S Chulpanova, Kristina V Kitaeva, Leysan G Tazetdinova, Victoria James, Albert A Rizvanov, Valeriya V Solovyeva
Mesenchymal stem cells (MSCs) are non-hematopoietic progenitor cells, which can be isolated from different types of tissues including bone marrow, adipose tissue, tooth pulp, and placenta/umbilical cord blood. There isolation from adult tissues circumvents the ethical concerns of working with embryonic or fetal stem cells, whilst still providing cells capable of differentiating into various cell lineages, such as adipocytes, osteocytes and chondrocytes. An important feature of MSCs is the low immunogenicity due to the lack of co-stimulatory molecules expression, meaning there is no need for immunosuppression during allogenic transplantation...
2018: Frontiers in Pharmacology
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