keyword
MENU ▼
Read by QxMD icon Read
search

Chondrocyte transplant

keyword
https://www.readbyqxmd.com/read/29452287/dental-pulp-stem-cell-derived-chondrogenic-cells-demonstrate-differential-cell-motility-in-type-i-and-type-ii-collagen-hydrogels
#1
Li Yao, Nikol Flynn
BACKGROUND CONTEXT: Advances in the development of biomaterials and stem cell therapy provide a promising approach to regenerating degenerated discs. The normal nucleus pulposus (NP) cells exhibit the similar phenotype as chondrocytes. Because dental pulp stem cells (DPSCs) can be differentiated into chondrogenic cells, the DPSCs and DPSCs-derived chondrogenic cells encapsulated in type I and type II collagen hydrogels can potentially be transplanted into degenerated nucleus pulposus (NP) to repair damaged tissue...
February 13, 2018: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/29436582/transplantation-of-allogenic-nucleus-pulposus-cells-attenuates-intervertebral-disc-degeneration-by-inhibiting-apoptosis-and-increasing-migration
#2
Weiheng Wang, Guoying Deng, Yuanyuan Qiu, Xiaodong Huang, Yanhai Xi, Jiangming Yu, Xiangqun Yang, Xiaojian Ye
Transplantation of nucleus pulposus cells (NPCs) into the intervertebral disc (IVD) has been demonstrated to be an effective treatment of degenerative disc disease (DDD). However, the underlying mechanisms have remained to be sufficiently elucidated. The aim of the present study was to explore the potential cell migration and anti‑apoptosis efficacy of NPCs in the treatment of DDD. NPCs cultured from rats expressing green fluorescent protein (GFP‑NPCs) were transplanted into the degenerated IVD, and the migration of GFP‑NPCs, as well as the degeneration and apoptosis of the IVD were detected to evaluate the therapeutic effect in vivo...
February 2, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29429373/long-term-clinical-and-mri-results-of-matrix-assisted-autologous-chondrocyte-implantation-for-articular-cartilage-defects-of-the-knee
#3
Peter Cornelius Kreuz, Richard Horst Kalkreuth, Philipp Niemeyer, Markus Uhl, Christoph Erggelet
Objective To evaluate the long-term clinical and radiological outcome of matrix-assisted autologous chondrocyte implantation (mACI) for articular cartilage defects in the knee joint. Design Clinical evaluation was assessed in 21 patients with full-thickness cartilage defects, International Cartilage Repair Society (ICRS) grade IV. Clinical scoring was performed preoperatively and 12 years after transplantation using the International Knee Documentation Committee (IKDC) score, the Lysholm score, the Knee injury and Osteoarthritis Outcome Score (KOOS), and the Noyes sports activity rating scale...
February 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29390874/optimized-longitudinal-monitoring-of-stem-cell-grafts-in-mouse-brain-using-a-novel-bioluminescent-near-infrared-fluorescent-fusion-reporter
#4
Laura Mezzanotte, Juvita Delancy Iljas, Ivo Que, Alan Chan, Eric Kaijzel, Rob Hoeben, Clemens Löwik
Biodistribution and fate of transplanted stem cells via longitudinal monitoring has been successfully achieved in the last decade using optical imaging. However, sensitive longitudinal imaging of transplanted stem cells in deep tissue like the brain remains challenging not only due to low light penetration but because of other factors such as low or inferior expression levels of optical reporters in stem cells and stem cell death after transplantation. Here we describe an optimized imaging protocol for sensitive long-term monitoring of bone marrow-derived human mesenchymal stem cells (hMSCs) expressing a novel bioluminescent/near infrared fluorescent (NIRF) fusion reporter transplanted in mouse brain cortex...
December 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29346562/scaffold-free-trachea-regeneration-by-tissue-engineering-with-bio-3d-printing
#5
Daisuke Taniguchi, Keitaro Matsumoto, Tomoshi Tsuchiya, Ryusuke Machino, Yosuke Takeoka, Abdelmotagaly Elgalad, Kiyofumi Gunge, Katsunori Takagi, Yasuaki Taura, Go Hatachi, Naoto Matsuo, Naoya Yamasaki, Koichi Nakayama, Takeshi Nagayasu
OBJECTIVES: Currently, most of the artificial airway organs still require scaffolds; however, such scaffolds exhibit several limitations. Alternatively, the use of an autologous artificial trachea without foreign materials and immunosuppressants may solve these issues and constitute a preferred tool. The rationale of this study was to develop a new scaffold-free approach for an artificial trachea using bio-3D printing technology. Here, we assessed the circumferential tracheal replacement using scaffold-free trachea-like grafts generated from isolated cells in an inbred animal model...
January 15, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29345353/hipop-mesenchymal-population-has-high-potential-for-repairing-injured-peripheral-nerves
#6
Yukako Yamauchi, Shousaku Itoh, Haruna Naruse, Yuki Itoh, Makoto Abe, Takumi Kagioka, Mikako Hayashi
Bone marrow stromal cells (BMSCs) are reportedly a heterogeneous population of mesenchymal stem cells (MSCs). Recently, we developed a simple strategy for the enrichment of MSCs with the capacity to differentiate into osteoblasts, chondrocytes, and adipocytes. On transplantation, the progenitor-enriched fractions can regenerate the bone with multiple lineages of donor origin and are thus called "highly purified osteoprogenitors" (HipOPs). Although our previous studies have demonstrated that HipOPs are enriched with MSCs and exhibit a higher potential to differentiate into osteoblasts, adipocytes, and chondrocytes than BMSCs, their potential to differentiate into neural cells has not been clarified...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29328886/osteochondral-graft-size-is-significantly-associated-with-increased-force-and-decreased-chondrocyte-viability
#7
Brian E Walczak, Matthew S Nies, Darrin J Trask, Scott Hetzel, Patrick J Roney, Matthew W Squire, Geoffrey S Baer
BACKGROUND: Insertion force has been shown to significantly reduce chondrocyte viability during osteochondral allograft transplantation. How graft size influences the required insertion force and chondrocyte viability has yet to be determined. Hypothesis/Purpose: The purpose was to characterize how graft size influences insertion force requirements and chondrocyte viability during osteochondral transplantation. The hypothesis was that larger grafts would require greater force and reduce chondrocyte viability...
January 1, 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29300225/articular-cartilage-injury-pathways-and-treatment-options
#8
Timothy M Simon, Douglas W Jackson
Articular cartilage injury and degeneration is a frequent occurrence in synovial joints. Treatment of these articular cartilage lesions are a challenge because this tissue is incapable of quality repair and/or regeneration to its native state. Nonoperative treatments endeavor to control symptoms, and include anti-inflammatory medication, viscosupplementation, bracing, orthotics, and activity modification. Techniques to stimulate the intrinsic repair (fibrocartilage) process include drilling, abrasion, and microfracture of the subchondral bone...
March 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29281950/kartogenin-enhanced-chondrogenesis-in-co-cultures-of-chondrocytes-and-bone-mesenchymal-stem-cells
#9
Chun Liu, Tao Li, Zhijian Yang, Deshuai Liu, Yun Li, Zhiyou Zhou, Qiqing Zhang
Articular cartilage has poor capability of regeneration due to the avascular surrounding and low metabolic activity. Kartogenin (KGN), an emerging nonprotein heterocyclic compound, was screened to stimulate chondrogenic differentiation of bone mesenchymal stem cells (BMSCs). Co-culturing BMSCs and chondrocytes was reported to overcome the shortcomings of forming fibroblastic and hypertrophic cartilages. In this study, KGN was incorporated into the Col-Tgel hydrogel to form a Gel/Cell/KGN complex which fabricated an appropriate microenvironment for effective cartilage regeneration of BMSCs and/or chondrocyte...
December 27, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29259721/characterization-of-polydactyly-derived-chondrocyte-sheets-versus-adult-chondrocyte-sheets-for-articular-cartilage-repair
#10
Miki Maehara, Masato Sato, Eriko Toyoda, Takumi Takahashi, Eri Okada, Tomomi Kotoku, Masahiko Watanabe
Background: We previously conducted a first-in-human clinical study of articular cartilage repair using autologous chondrocyte sheets and confirmed the regeneration of hyaline-like cartilage in all eight patients. However, regenerative medicine with autologous chondrocyte sheets requires the harvesting of tissue from healthy regions, and the quality of this tissue varies between individuals. To overcome such limitations, allogeneic transplantation is a promising treatment method, particularly for articular cartilage repair...
2017: Inflammation and Regeneration
https://www.readbyqxmd.com/read/29228600/non-invasive-in-vivo-molecular-imaging-of-intra-articularly-transplanted-immortalized-bone-marrow-stem-cells-for-osteoarthritis-treatment
#11
Bou-Yue Peng, Chi-Sheng Chiou, Navneet Kumar Dubey, Sung-Hsun Yu, Yue-Hua Deng, Feng-Chou Tsai, Han-Sun Chiang, Ying-Hua Shieh, Wei-Hong Chen, Win-Ping Deng
Pathophysiology of osteoarthritis (OA) is characterized by progressive loss of articular cartilage in the knee-joints. To impart regenerative ability in lowly metabolizing chondrocytes, the bone marrow stem cells (BMSCs) has recently been recognized as a superior alternative treatment for OA. However, study of primary BMSCs-mediated chondrogenesis is difficult due to progressive cellular aging and replicative senescence. To obtain a therapeutic cell population for OA, BMSCs were immortalized by human papilloma virus (HPV)-16 E6/E7 along with mCherry luciferase (mCL), a gene marker for non-invasive imaging, and designated as iBMSCs-mCL...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29228404/chondrocyte-viability-at-time-of-transplantation-for-osteochondral-allografts-preserved-by-the-missouri-osteochondral-preservation-system-versus-standard-tissue-bank-protocol
#12
Aaron M Stoker, James P Stannard, James L Cook
No abstract text is available yet for this article.
December 11, 2017: Journal of Knee Surgery
https://www.readbyqxmd.com/read/29218684/feasibility-of-arthroscopic-autologous-chondrocyte-implantation-in-the-hip-using-an-injectable-hydrogel
#13
Steffen Thier, Florian Baumann, Christel Weiss, Stefan Fickert
INTRODUCTION: In the long term the treatment of articular cartilage defects of the hip has the most direct impact on the postoperative outcome and should diminish degenerative changes caused by different pathologies. The purpose of this prospective feasibility study is to describe technical aspects of arthroscopic, injectable autologous chondrocyte implantation in the hip and to report the short-term outcome. METHODS: Full-thickness cartilage defects of 13 patients were treated arthroscopically with an injectable autologous chondrocyte transplantation product (Novocart Inject, Tetec) in a 2-step surgical procedure...
December 8, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29198358/editorial-commentary-the-acellular-osteochondral-allograft-the-emperor-has-new-clothes
#14
EDITORIAL
Bert R Mandelbaum, Jorge Chahla
For larger lesions (>2.5-cm2), clinical evidence and practice have shown that fresh osteochondral allograft have good durability, with 88% return to sport and greater than 75% 10-year survival rates for treatment of large femoral condyle lesions. That said, the use of fresh osteochondral allografts in clinical practice is limited by the availability of acceptable donor tissues for eligible patients in a timely fashion. Significant diminution of chondrocyte viability and density occurs during the preservation and storage period...
December 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/29190645/cd105-mesenchymal-stem-cells-migrate-into-osteoarthritis-joint-an-animal-model
#15
Pablo Fernandez-Pernas, Iván Rodríguez-Lesende, Alexandre de la Fuente, Jesús Mateos, Isaac Fuentes, Javier De Toro, Fco J Blanco, M C Arufe
Mesenchymal stem cells are being the focus of connective tissue technology and regenerative medicine, presenting a good choice cell source for improving old and well recognized techniques of cartilage defect repair. For instance, the autologous chondrocyte transplantation using new concepts of regenerative medicine. The present study investigated the risk of xenogenicity of human synovial membrane-derived MSCs, injected into the monkeys using intravenous and intra-articular administration. The animal models used were adult monkeys Rhesus which had been injured into the left knee to create an Osteoarthritis (OA) animal model...
2017: PloS One
https://www.readbyqxmd.com/read/29177132/autologous-cell-seeding-in-tracheal-tissue-engineering
#16
REVIEW
Elizabeth F Maughan, Robert E Hynds, Toby J Proctor, Sam M Janes, Martin Elliott, Martin A Birchall, Mark W Lowdell, Paolo De Coppi
Purpose of Review: There is no consensus on the best technology to be employed for tracheal replacement. One particularly promising approach is based upon tissue engineering and involves applying autologous cells to transplantable scaffolds. Here, we present the reported pre-clinical and clinical data exploring the various options for achieving such seeding. Recent Findings: Various cell combinations, delivery strategies, and outcome measures are described. Mesenchymal stem cells (MSCs) are the most widely employed cell type in tracheal bioengineering...
2017: Current Stem Cell Reports
https://www.readbyqxmd.com/read/29160845/vascular-endothelial-growth-factor-sequestration-enhances-in-vivo-cartilage-formation
#17
Carolina M Medeiros Da Cunha, Valeria Perugini, Petra Bernegger, Matteo Centola, Andrea Barbero, Anna L Guildford, Matteo Santin, Andrea Banfi, Ivan Martin, Anna Marsano
Autologous chondrocyte transplantation for cartilage repair still has unsatisfactory clinical outcomes because of inter-donor variability and poor cartilage quality formation. Re-differentiation of monolayer-expanded human chondrocytes is not easy in the absence of potent morphogens. The Vascular Endothelial Growth Factor (VEGF) plays a master role in angiogenesis and in negatively regulating cartilage growth by stimulating vascular invasion and ossification. Therefore, we hypothesized that its sole microenvironmental blockade by either VEGF sequestration by soluble VEGF receptor-2 (Flk-1) or by antiangiogenic hyperbranched peptides could improve chondrogenesis of expanded human nasal chondrocytes (NC) freshly seeded on collagen scaffolds...
November 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29143065/-complex-joint-reconstruction-and-joint-transplantation-with-the-flocsat-concept-planning-and-surgical-implementation
#18
REVIEW
C Krettek, J-D Clausen, C Neunaber
Cartilage defects in adult patients are so far incurable. Fresh osteochondral allograft (OCA) transplantation is based on the insertion of mature, living, mechanically sound hyaline cartilage into an osteochondral defect where it undergoes osseointegration. Intact hyaline cartilage of OCA does not cause immune reactions in the recipient. Many reports show that small OCA have good osseointegration and show good long-term results. These observations have been incorporated into the development of the fresh large (> 10 cm(2)) osteochondral shell allograft transplantation (FLOCSAT) concept, which is based on the following principles: 1) the thickness of the osseous layer should be kept as thin as possible (target < 6-8 mm) so that the transplant remains stable and fixable...
November 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/29137495/development-of-chondrocyte-seeded-electrosprayed-nanoparticles-for-repair-of-articular-cartilage-defects-in-rabbits
#19
Shan-Wei Yang, Kai-Chi Ku, Shu-Ying Chen, Shyh-Ming Kuo, I-Fen Chen, Ting-Yi Wang, Shwu-Jen Chang
Due to limited self-healing capacity in cartilages, there is a rising demand for an innovative therapy that promotes chondrocyte proliferation while maintaining its biofunctionality for transplantation. Chondrocyte transplantation has received notable attention; however, the tendencies of cell de-differentiation and de-activation of biofunctionality have been major hurdles in its development, delaying this therapy from reaching the clinic. We believe it is due to the non-stimulative environment in the injured cartilage, which is unable to provide sustainable physical and biological supports to the newly grafted chondrocytes...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/29131029/rapid-induction-of-osteogenic-markers-in-mesenchymal-stem-cells-by-adipose-derived-stromal-vascular-fraction-cells
#20
Jung-Won Choi, Sunhye Shin, Chang Youn Lee, Jiyun Lee, Hyang-Hee Seo, Soyeon Lim, Seahyoung Lee, Il-Kwon Kim, Hoon-Bum Lee, Sang Woo Kim, Ki-Chul Hwang
BACKGROUND/AIMS: Stromal vascular fraction (SVF) cells are a mixed cell population, and their regenerative capacity has been validated in various therapeutic models. The purpose of this study was to investigate the regenerative mechanisms utilized by implanted SVF cells. Using an in vitro co-culture system, we sought to determine whether SVF implantation into impaired tissue affects endogenous mesenchymal stem cell (MSC) differentiation; MSCs can differentiate into a variety of cell types, and they have a strong regenerative capacity despite their low numbers in impaired tissue...
November 3, 2017: Cellular Physiology and Biochemistry
keyword
keyword
48570
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"