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https://www.readbyqxmd.com/read/28548821/turning-nonspecific-interference-into-signal-amplification-covalent-biosensing-nanoassembly-enabled-by-metal-catalyzed-cross-coupling
#1
Hao Li, Fang Wang, Shenguang Ge, Haiyun Liu, Mei Yan, Jinghua Yu
In this work, a new method of protein detection in complicated samples is proposed. This method employs probe-target recognition to induce cross-linking among the probe, the target, and the nonspecific proteins in the complicated sample as a means to convert interference into effective signal amplification. This also eliminates the necessity of multistep signal amplification in a separate solution system. On the basis of this strategy, a simple and robust assay for the activity of serum cathepsin B is established...
May 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28544921/in-situ-formed-copper-nanoparticles-templated-by-tdt-mediated-dna-for-enhanced-spr-sensor-based-dna-assay
#2
Pei-Xin Yuan, Sheng-Yuan Deng, Chen-Yu Zheng, Serge Cosnier, Dan Shan
For the efficient surface plasmon resonance (SPR)-based DNA assay researching, signal amplification tactics were absolutely necessary. In this work, a sensitive SPR-DNA sensor was developed by employing in situ synthesis of copper nanoparticles (CuNPs) templated by poly-T sequences DNA from terminal deoxynucleotidyl transferase (TdT)-mediated extension, and synergistically with nano-effect deposition as the mass relay. The objective of this strategy was manifold: firstly, tDNA hybridized with the optimal designed probes to active the TdT-mediated DNA extension onto the surface of SPR chip, resulted a long poly-T sequences ssDNA chain in dsDNA terminal onto surface of gold chip and characterized by SPR signal amplitudes...
May 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28542570/evolutionary-morphology-in-shape-and-size-of-haptoral-anchors-in-14-ligophorus-spp-monogenea-dactylogyridae
#3
Abril Rodríguez-González, Volodimir Sarabeev, Juan Antonio Balbuena
The search for phylogenetic signal in morphological traits using geometric morphometrics represents a powerful approach to estimate the relative weights of convergence and shared evolutionary history in shaping organismal form. We assessed phylogenetic signal in the form of ventral and dorsal haptoral anchors of 14 species of Ligophorus occurring on grey mullets (Osteichthyes: Mugilidae) from the Mediterranean, the Black Sea and the Sea of Azov. The phylogenetic relationships among these species were mapped onto the morphospaces of shape and size of dorsal and ventral anchors and two different tests were applied to establish whether the spatial positions in the morphospace were dictated by chance...
2017: PloS One
https://www.readbyqxmd.com/read/28542274/detecting-infection-hotspots-modeling-the-surveillance-challenge-for-elimination-of-lymphatic-filariasis
#4
Julie R Harris, Ryan E Wiegand
BACKGROUND: During the past 20 years, enormous efforts have been expended globally to eliminate lymphatic filariasis (LF) through mass drug administration (MDA). However, small endemic foci (microfoci) of LF may threaten the presumed inevitable decline of infections after MDA cessation. We conducted microsimulation modeling to assess the ability of different types of surveillance to identify microfoci in these settings. METHODS: Five or ten microfoci of radius 1, 2, or 3 km with infection marker prevalence (intensity) of 3, 6, or 10 times background prevalence were placed in spatial simulations, run in R Version 3...
May 19, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28540753/learning-from-where-eye-remotely-look-or-point-impact-on-number-line-estimation-error-in-adults
#5
Thomas Gallagher-Mitchell, Victoria Simms, Damien Litchfield
In this paper we present an investigation into the use of visual cues during number line estimation, and their influence on cognitive processes for reducing number line estimation error. Participants completed a 0-1000 number line estimation task pre and post a brief intervention in which they observed static-visual or dynamic-visual cues (control, anchor, gaze cursor, mouse cursor) and also made estimation marks to test effective number-target estimation. Results indicated that a significant pre-test to post-test reduction in estimation error was present for dynamic visual cues of modelled eye-gaze and mouse-cursor...
May 25, 2017: Quarterly Journal of Experimental Psychology: QJEP
https://www.readbyqxmd.com/read/28540724/pde%C3%AE-binding-to-ras-isoforms-provides-a-route-to-proper-membrane-localization
#6
Serena Muratcioglu, Hyunbum Jang, Attila Gursoy, Ozlem Keskin, Ruth Nussinov
To signal, Ras isoforms must be enriched at the plasma membrane (PM). It was suggested that phosphodiesterase-δ (PDEδ) can bind and shuttle some farnesylated Ras isoforms to the PM - but not all. Among these, interest focused on K-Ras4B, the most abundant oncogenic Ras isoform. To study PDEδ/Ras interactions, we modeled and simulated the PDEδ/K-Ras4B complex. We obtained structures which were similar to two subsequently determined crystal structures. We next modeled and simulated complexes of PDEδ with the farnesylated hypervariable regions (HVRs) of K-Ras4A and N-Ras...
May 25, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28539360/a-dock-and-coalesce-mechanism-driven-by-hydrophobic-interactions-governs-cdc42-binding-with-its-effector-protein-ack
#7
George J N Tetley, Helen R Mott, R Neil Cooley, Darerca Owen
Cdc42 is a Rho-family small G protein that has been widely studied for its role in controlling the actin cytoskeleton and plays a part in several potentially oncogenic signalling networks. Similar to most other small G proteins, Cdc42 binds to many downstream effector proteins to elicit its cellular effects. These effector proteins all engage the same face of Cdc42, the conformation of which is governed by the activation state of the G protein. Previously, the importance of individual residues in conferring binding affinity has been explored for residues within Cdc42 for three of its CRIB effectors, activated Cdc42 kinase (ACK), p21-activated kinase (PAK), and Wiskott-Aldrich syndrome protein (WASP)...
May 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28538526/responsiveness-and-minimal-important-changes-of-the-scoliosis-research-society-22-patient-questionnaire-in-subjects-with-mild-adolescent-and-moderate-adult-idiopathic-scoliosis-undergoing-multidisciplinary-rehabilitation
#8
Marco Monticone, Emilia Ambrosini, Barbara Rocca, Calogero Foti, Simona Ferrante
STUDY DESIGN: Single-center, prospective study. OBJECTIVE: Evaluating the responsiveness and minimal important changes (MICs) for the Scoliosis Research Society-22 Patient Questionnaire (SRS-22) in adolescent idiopathic scoliosis (AIS) and adult idiopathic scoliosis (AS). SUMMARY OF BACKGROUND DATA: Despite the SRS-22 properties have been investigated in various different languages, there is still a lack of information concerning responsiveness and MIC, limiting the use of SRS-22 for clinical and research purposes...
June 1, 2017: Spine
https://www.readbyqxmd.com/read/28536590/miniature-inverted-repeat-transposable-element-insertions-provide-a-source-of-intron-length-polymorphism-markers-in-the-carrot-daucus-carota-l
#9
Katarzyna Stelmach, Alicja Macko-Podgórni, Gabriela Machaj, Dariusz Grzebelus
The prevalence of non-autonomous class II transposable elements (TEs) in plant genomes may serve as a tool for relatively rapid and low-cost development of gene-associated molecular markers. Miniature inverted-repeat transposable element (MITE) copies inserted within introns can be exploited as potential intron length polymorphism (ILP) markers. ILPs can be detected by PCR with primers anchored in exon sequences flanking the target introns. Here, we designed primers for 209 DcSto (Daucus carota Stowaway-like) MITE insertion sites within introns along the carrot genome and validated them as candidate ILP markers in order to develop a set of markers for genotyping the carrot...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28536260/functional-role-of-the-three-conserved-cysteines-in-the-n-domain-of-visual-arrestin-1
#10
Sergey A Vishnivetskiy, Regina J Lee, X Edward Zhou, Andreas Franz, Qiuyi Xu, H Eric Xu, Vsevolod V Gurevich
Arrestins specifically bind active and phosphorylated forms of their cognate G protein-coupled receptors, blocking G protein coupling and often redirecting the signaling to alternative pathways. High-affinity receptor binding is accompanied by the two major structural changes in arrestin: the release of the C-tail and rotation of the two domains relative to each other. The first requires the detachment of the arrestin C-tail from the body of the molecule, whereas the second requires the disruption of the network of charge-charge interactions at the interdomain interface, termed the polar core...
May 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28535985/recalcitrant-deep-and-shallow-nodes-in-aristolochia-aristolochiaceae-illuminated-using-anchored-hybrid-enrichment
#11
Stefan Wanke, Carolina Granados Mendoza, Sebastian Müller, Anna Paizanni Guillén, Christoph Neinhuis, Alan R Lemmon, Emily Moriarty Lemmon, Marie-Stéphanie Samain
Recalcitrant relationships are characterized by very short internodes that can be found among shallow and deep phylogenetic scales all over the tree of life. Adding large amounts of presumably informative sequences, while decreasing systematic error, has been suggested as a possible approach to increase phylogenetic resolution. The development of enrichment strategies, coupled with next generation sequencing, resulted in a cost-effective way to facilitate the reconstruction of recalcitrant relationships. By applying the anchored hybrid enrichment (AHE) genome partitioning strategy to Aristolochia using an angiosperm probe set, we obtained 231 to 233 out of 517 single or low copy nuclear loci originally contained in the enrichment kit, resulting in a total alignment length of 154,756 bp to 160,150 bp...
May 20, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28535907/suppression-of-epithelial-restitution-using-an-inhibitor-against-rho-associated-coiled-coil-containing-protein-kinase-aggravates-colitis-through-reduced-epithelial-expression-of-a-kinase-anchor-protein-13
#12
Yumi Kangawa, Toshinori Yoshida, Yutaka Yonezawa, Kiyoshi Maruyama, Shim-Mo Hayashi, Makoto Shibutani
In the gastrointestinal tract, the immediate healing response to mucosal damage is critical to sustain mucosal homeostasis. The migration of surrounding epithelial cells to cover the denuded area without proliferation is termed restitution, followed by early reparation of the damage. In this study, we determined the role of A-kinase anchor protein 13 (AKAP13) in mice with dextran sulphate sodium (DSS)-induced colitis upon mucosal injury and restitution, and investigated whether inhibition of Rho-associated coiled-coil containing protein kinase (ROCK), downstream effector of AKAP13, affects these mucosal responses...
May 20, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28535354/high-capacity-and-ultrafast-na-ion-storage-of-self-supported-3d-porous-antimony-persulfide-graphene-foam-architecture
#13
Yanying Lu, Ning Zhang, Shuang Jiang, Yudong Zhang, Meng Zhou, Zhanliang Tao, Lynden A Archer, Jun Chen
The key challenge for high performance sodium-ion batteries is the exploitation of appropriate electrode materials with long cycling stability and high rate capability. Here, we report Sb2S5 nanoparticles (~ 5 nm) uniformly encapsulated in three-dimensional (3D) porous graphene foam, which were fabricated by a facile hydrothermal co-assembly strategy, as a high-performance anode material for sodium-ion batteries. The as-prepared composite can be directly used as electrodes without adding binder or current collector, exhibiting outstanding electrochemical performance with high reversible capacity (845 mAh g-1 at 0...
May 23, 2017: Nano Letters
https://www.readbyqxmd.com/read/28535337/overall-structural-model-of-ns5a-protein-from-hepatitis-c-virus-and-modulation-by-mutations-confering-resistance-of-virus-replication-to-cyclosporin-a
#14
Aurelie Badillo, Veronique Brechot, Stephane Sarrazin, François-Xavier Cantrelle, Frederic Delolme, Marie-Laure Fogeron, Jennifer Molle, Roland Montserret, Anja Bockmann, Ralf Bartenschlager, Volker Lohmann, Guy Lippens, Sylvie Ricard-Blum, Xavier Hanoulle, Francois Penin
Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) is a RNA-binding phosphoprotein composed of a N-terminal membrane anchor (AH), a structured domain 1 (D1) and two intrinsically disordered domains (D2 and D3). The knowledge of the functional architecture of this multifunctional protein remains limited. We report here that NS5A-D1D2D3 produced in a wheat germ cell-free system is obtained under a highly phosphorylated state. Its NMR analysis revealed that these phosphorylations do not change the disordered nature of D2 and D3 domains but increase the number of conformers due to partial phosphorylations...
May 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/28535054/tuning-the-binding-dynamics-of-a-guest-octaacid-capsule-through-noncovalent-anchoring
#15
Suma S Thomas, Hao Tang, Adam Gaudes, Signe B Baggesen, Corinne L D Gibb, Bruce C Gibb, Cornelia Bohne
Hydrophobic or hydrophilic substituents have different effects on the binding dynamics of pyrene derivatives with a 2:1 capsule formed from two octaacid cavitands, showing a subtle interplay of different kinetic factors. Anchoring of the methyl group of 1-methylpyrene within one cavitand slowed the association and dissociation dynamics of the 1:1 complex by at least 1000 times when compared to the 1:1 complex for pyrene. This slow down for the transient formation of the 1:1 complex is responsible for the overall increase in stability of the 2:1 complex without affecting the overall capsule dissociation...
May 25, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28533751/docetaxel-loaded-nanoparticles-assembled-from-%C3%AE-cyclodextrin-calixarene-giant-surfactants-physicochemical-properties-and-cytotoxic-effect-in-prostate-cancer-and-glioblastoma-cells
#16
Laura Gallego-Yerga, Inmaculada Posadas, Cristina de la Torre, Jesús Ruiz-Almansa, Francesco Sansone, Carmen Ortiz Mellet, Alessandro Casnati, José M García Fernández, Valentín Ceña
Giant amphiphiles encompassing a hydrophilic β-cyclodextrin (βCD) component and a hydrophobic calix[4]arene (CA4) module undergo self-assembly in aqueous media to afford core-shell nanospheres or nanocapsules, depending on the nanoprecipitation protocol, with high docetaxel (DTX) loading capacity. The blank and loaded nanoparticles have been fully characterized by dynamic light scattering (DLS), ζ-potential measurements and cryo-transmission electron microscopy (cryo-TEM). The data are compatible with the distribution of the drug between the nanoparticle core and the shell, where it is probably anchored by inclusion of the DTX aromatic moieties in βCD cavities...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28533009/comparison-of-implant-stability-measurement-devices-for-bone-anchored-hearing-aid-systems
#17
Lindsey Westover, Gary Faulkner, William Hodgetts, Don Raboud
STATEMENT OF PROBLEM: The success of implants for bone-anchored hearing aids (BAHA) relies on proper osseointegration at the bone-implant interface. Clinical evaluation of implant stability is important in prescribing loading, identifying the risk of failure, and monitoring the long-term health of the implant. PURPOSE: The purpose of this in vitro study was to evaluate 2 measurement systems for BAHA implant stability: the most commonly used, Osstell implant stability quotient (ISQ), and a newly developed advance system for implant stability testing (ASIST)...
May 20, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28527966/suture-dehiscence-in-the-tricuspid-annulus-an-ex%C3%A2-vivo-analysis-of-tissue-strength-and-composition
#18
Deborah M Paul, Ajay Naran, Eric L Pierce, Charles H Bloodworth, Steven F Bolling, Ajit P Yoganathan
BACKGROUND: Surgical repair of functional tricuspid regurgitation (FTR) is an increasingly common practice, but annuloplasty suture dehiscence remains a significant problem. Quantitative and mechanistic understanding of annular suture holding strength can support more effective techniques for tricuspid valve device anchoring. METHODS: Suture holding strength of ovine tricuspid annuli (n = 15) was quantified ex vivo by pullout testing at 12 positions around their circumference...
May 17, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28527947/embryonic-vascular-disruption-adverse-outcomes-linking-high-throughput-signaling-signatures-with-functional-consequences
#19
Robert G Ellis-Hutchings, Raja S Settivari, Alene T McCoy, Nicole Kleinstreuer, Jill Franzosa, Thomas B Knudsen, Edward W Carney
Embryonic vascular disruption is an important adverse outcome pathway (AOP) as chemical disruption of cardiovascular development induces broad prenatal defects. High throughput screening (HTS) assays aid AOP development although linking in vitro data to in vivo apical endpoints remains challenging. This study evaluated two anti-angiogenic agents, 5HPP-33 and TNP-470, across the ToxCastDB HTS assay platform and anchored the results to complex in vitro functional assays: the rat aortic explant assay (AEA), rat whole embryo culture (WEC), and the zebrafish embryotoxicity (ZET) assay...
May 17, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28527749/visual-and-proprioceptive-feedback-differently-modulate-the-spatial-representation-of-number-and-time-in-children
#20
Elena Nava, Luca Rinaldi, Hermann Bulf, Viola Macchi Cassia
There has been compelling evidence favoring the idea that human adults similarly represent number and time along a horizontal mental number line (MNL) and mental time line (MTL), respectively. Yet, analogies drawn between the MNL and MTL have been challenged by recent studies suggesting that adults' representations of number and time arise from different spatial frames of reference; whereas the MNL relies on both hand-centered and object-centered coordinates, the MTL appears to be exclusively anchored on object-centered coordinates...
May 18, 2017: Journal of Experimental Child Psychology
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