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https://www.readbyqxmd.com/read/29336990/failure-of-intracardiac-pacing-after-fatal-propafenone-overdose-a-case-report
#1
Ivan Zeljković, Nikola Bulj, Matea Kolačević, Vedran Čabrilo, Diana Delić Brkljačić, Šime Manola
BACKGROUND: Propafenone is a sodium-channel blocker, class IC antiarrhythmic drug, frequently used to manage supraventricular dysrhythmias, especially atrial fibrillation. We report a self mono-intoxication with propafenone. CASE REPORT: A 68-year-old woman presented with a decreased level of consciousness, hypotension, and electrocardiogram showing QRS widening with atrial asystole and extreme bradycardia < 20 beats/min. After initial stabilization with transcutaneous pacing, laboratory findings detected normal electrolyte ranges and metabolic acidosis, and her medical history revealed availability of propafenone due to paroxysmal atrial fibrillation and depressive syndrome, which led to the suspicion of intoxication...
January 11, 2018: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29331472/influence-of-partially-demineralized-milk-proteins-on-rheological-properties-and-microstructure-of-acid-gels
#2
G H Meletharayil, H A Patel, L E Metzger, C Marella, T Huppertz
Innovative clean label processes employed in the manufacture of acid gels are targeted to modify the structure of proteins that contribute to rheological properties. In the present study, CO2-treated milk protein concentrate powder with 80% protein in dry matter (TMPC80) was mixed with nonfat dry milk (NDM) in different ratios for the manufacture of acid gels. Dispersions of NDM and TMPC80 that provided 100, 90, 70, and 40% of protein from NDM were reconstituted to 4.0% (wt/wt) protein and 12.0% (wt/wt) total solids...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29326512/evaluating-the-effect-of-different-conditioning-agents-on-the-shear-bond-strength-of-resin-modified-glass-ionomers
#3
Namith Rai, Rajaram Naik, Ravi Gupta, Shobana Shetty, Amith Singh
Aim of the Study: This study aims to evaluate the effects three different conditioning agents on the shear bond strength of resin-modified glass ionomers to human dentin. Materials and Methods: One hundred and twenty recently extracted, caries-free premolars and molars will be cleaned of debris and disinfected in a 0.5% solution of sodium hypochlorite and sterile water for 30 min. The occlusal surface of each tooth will be reduced using conventional model trimmer with water to produce the dentin surface...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29322611/impact-of-pre-procedural-hyponatremia-on-clinical-outcomes-after-transcatheter-aortic-valve-replacement-a-propensity-matched-analysis
#4
Ai Kagase, Masanori Yamamoto, Tetsuro Shimura, Atsuko Kodama, Seiji Kano, Yutaka Koyama, Norio Tada, Toru Naganuma, Motoharu Araki, Futoshi Yamanaka, Shinichi Shirai, Yusuke Watanabe, Kentaro Hayashida
BACKGROUND: Hyponatremia is associated with the increased risk of early and late mortality in patients with cardiac disease. This study aimed to assess the prognostic value of hyponatremia in patients who had undergone transcatheter aortic valve replacement (TAVR). METHODS: We investigated 1,215 consecutive patients (mean age: 84.4 ± 5.0 years) who underwent TAVR using data from the Optimized CathEter vAlvular iNtervention (OCEAN)-TAVR Japanese multicenter registry...
January 11, 2018: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29310856/chemoenzymatic-synthesis-of-sucuronic-acid-using-d-glucurono-6-3-lactone-and-sucrose-as-raw-materials-and-properties-of-the-product
#5
Hiroki Hosaka, Saori Mizoguchi, Mitsuru Tashiro, Takashi Fujimoto, Takako Hirano, Wataru Hakamata, Toshiyuki Nishio
Using d-glucurono-6,3-lactone (GlcL) and sucrose (Suc) as raw materials, we synthesized sucuronic acid (SucA), in which the d-glucose (Glc) residue of Suc was replaced with d-glucuronic acid, by a three-step chemoenzymatic method. In the 1st chemical step, methyl d-glucuronate (GlcAM) was synthesized by treating GlcL with a strong base anion exchange resin, Amberlite IRA402BL OH AG, in anhydrous methanol. In the 2nd step, which included an enzyme reaction, methyl sucuronate (SucAM) was synthesized from GlcAM and fructose by exploiting the transfructosylation activity of the Microbacterium saccharophilum K-1 β-fructofuranosidase, a reaction that is suppressed in the presence of high-concentration Glc...
March 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29310404/extracorporeal-membrane-oxygenation-combined-with-continuous-renal-replacement-therapy-in-cutaneous-burn-and-inhalation-injury-caused-by-hydrofluoric-acid-and-nitric-acid
#6
Qinhua Pu, Jinxian Qian, Weiyi Tao, Aixiang Yang, Jian Wu, Yaodong Wang
RATIONALE: Hydrofluoric acid (HF) is a highly corrosive agent and can cause corrosive burns. HF can penetrate deeply into tissues through intact skin and the lipid barrier, leading to painful liquefactive necrosis, and inducing hypocalcemia and hypomagnesemia. In this study, we hypothesize that continuous renal replacement therapy (CRRT) may be beneficial in addressing hemodynamic instability in cases of HF poisoning. PATIENT CONCERNS: A 25-year-old man fell into an electroplating pool containing 10% HF and 50% nitric acid...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29306662/ciguatoxins-activate-the-calcineurin-signalling-pathway-in-yeasts-potential-for-development-of-an-alternative-detection-tool
#7
Hélène Martin-Yken, Camille Gironde, Sylvain Derick, Hélène Taiana Darius, Christophe Furger, Dominique Laurent, Mireille Chinain
Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdiscus spp. and ciguatoxic fishes have been recently identified in the North Atlantic Ocean (Madeira and Canary islands) and Mediterranean Sea. Ciguatoxins bind to Voltage Gated Sodium Channels on the membranes of sensory neurons, causing Ciguatera Fish Poisoning (CFP) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms...
January 4, 2018: Environmental Research
https://www.readbyqxmd.com/read/29304756/medical-treatment-of-an%C3%A2-unusual-cerebral-hydatid-disease
#8
Shu Chen, Ning Li, Feifei Yang, Jiqin Wu, Yuekai Hu, Shenglei Yu, Qi Chen, Xuan Wang, Xinyu Wang, Yuanyuan Liu, Jianming Zheng
BACKGROUND: Hydatid disease is a worldwide zoonosis produced by the larval stage of cestodes of the Echinococcus genus. Hydatid disease primarily involves the liver and lungs. The brain is involved in less than 2% of cases. Surgery has long been the only choice for the treatment, but chemotherapy has been successfully replaced surgery in some special cases. CASE PRESENTATION: We report a rare hydatid disease case which presented with multiple lesions in right frontal lobe, an uncommon site, and in the liver and lungs...
January 5, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29299569/influence-of-the-manganese-and-cobalt-content-on-the-electrochemical-performance-of-p2-na0-67mnxco1-xo2-cathodes-for-sodium-ion-batteries
#9
K Hemalatha, M Jayakumar, A S Prakash
The resurgence of sodium-ion batteries in recent years is due to their potential ability to form intercalation compounds possessing a high specific capacity and energy density comparable to existing lithium systems. To comprehend the role of cobalt substitution in the structure and electrochemical performance of Na0.67MnO2, the solid solutions of P2-Na0.67MnxCo1-xO2 (x = 0.25, 0.5, 0.75) are synthesized and characterized. The XRD-Rietveld analysis revealed that the Co-substitution in Na0.67MnO2 decreases lattice parameters 'a' and 'c' resulting in the contraction of MO6 octahedra and the enlargement of inter-layer 'd' spacing...
January 4, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29295898/preparation-of-plasmid-dna-by-alkaline-lysis-with-sodium-dodecyl-sulfate-maxipreps
#10
Michael R Green, Joseph Sambrook
In recent years, with the advent of polymerase chain reaction and the development of highly efficient methods of cloning and DNA sequencing, the need to prepare large quantities of plasmid vectors and recombinants has greatly diminished. In consequence, this protocol, at one time in common use, has been largely replaced by faster and easier column-based purification methods. In this protocol, plasmid DNA is purified from the cleared bacterial lysate by centrifugation to equilibrium in CsCl gradients containing ethidium bromide...
January 2, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29291499/the-facile-removal-of-ctab-from-the-surface-of-gold-nanorods
#11
Jie He, Sarah Unser, Ian Bruzas, ReJeana Cary, Zhiwei Shi, Rajesh Mehra, Kenneth Aron, Laura Sagle
A common capping agent for gold nanorods, Cetyl trimethylammonium bromide (CTAB), is particularly problematic for biological studies because of its cytotoxicity. Several procedures have been developed to remove the CTAB from the surface of the gold nanorods, but most are lengthy, involving many steps, and use expensive reagents. Here, we present a simple, one-pot method for the complete removal of CTAB from the surface of gold nanorods, so that particles can be more effectively utilized in biological in vivo studies...
December 19, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29286066/effects-of-erk1-2-s-nitrosylation-on-erk1-2-phosphorylation-and-cell-survival-in-glioma-cells
#12
Lei Jin, Yujia Cao, Tong Zhang, Peng Wang, Daofei Ji, Xuejiao Liu, Hengliang Shi, Lei Hua, Rutong Yu, Shangfeng Gao
Aberrant activation of extracellular signal‑regulated kinase 1/2 (ERK1/2) by phosphorylation modification can trigger tumor cell development in glioma. S‑nitrosylation, which refers to the covalent addition of a nitric oxide (NO) group to a cysteine (Cys) thiol, is an important post‑translational modification that occurs on numerous cancer‑associated proteins. Protein S‑nitrosylation can increase or decrease protein activity and stability, and subsequent signal transduction and cellular processes...
December 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29282153/under-control-how-a-dietary-additive-can-restore-the-gut-microbiome-and-proteomic-profile-and-improve-disease-resilience-in-a-marine-teleostean-fish-fed-vegetable-diets
#13
María Carla Piazzon, Josep Alvar Calduch-Giner, Belén Fouz, Itziar Estensoro, Paula Simó-Mirabet, Mónica Puyalto, Vasileios Karalazos, Oswaldo Palenzuela, Ariadna Sitjà-Bobadilla, Jaume Pérez-Sánchez
BACKGROUND: The constant increase of aquaculture production and wealthy seafood consumption has forced the industry to explore alternative and more sustainable raw aquafeed materials, and plant ingredients have been used to replace marine feedstuffs in many farmed fish. The objective of the present study was to assess whether plant-based diets can induce changes in the intestinal mucus proteome, gut autochthonous microbiota and disease susceptibility of fish, and whether these changes could be reversed by the addition of sodium butyrate to the diets...
December 28, 2017: Microbiome
https://www.readbyqxmd.com/read/29274525/in-vitro-biomechanical-and-hydrodynamic-characterisation-of-decellularised-human-pulmonary-and-aortic-roots
#14
A Desai, T Vafaee, P Rooney, J N Kearney, H E Berry, E Ingham, J Fisher, L M Jennings
BACKGROUND AND PURPOSE OF THE STUDY: The use of decellularised biological heart valves in the replacement of damaged heart valves offers a promising solution to reduce the degradation issues associated with existing cryopreserved allografts. The purpose of this study was to assess the effect of low concentration sodium dodecyl sulphate decellularisation on the in vitro biomechanical and hydrodynamic properties of cryopreserved human aortic and pulmonary roots. METHOD: The biomechanical and hydrodynamic properties of cryopreserved decellularised human aortic and pulmonary roots were fully characterised and compared to cellular human aortic and pulmonary roots in an unpaired study...
September 14, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29250609/spellbinding-effects-of-the-acidic-cooh-terminus-of-factor-va-heavy-chain-on-prothrombinase-activity-and-function
#15
Jamila Hirbawi, Michael Kalafatis
Human factor Va (hfVa) is the important regulatory subunit of prothrombinase. Recent modeling data have suggested a critical role for amino acid Arg701 of hfVa for human prothrombin (hPro) activation by prothrombinase. Furthermore, it has also been demonstrated that hfVa has a different effect than that of bovine fVa on prethrombin-1 activation by prothrombinase. The difference between the two cofactor molecules was also found within the Asn700-Arg701 dipeptide in the human factor V (hfV) molecule, which is replaced by the Asp-Glu sequence in bfV...
September 30, 2017: ACS Omega
https://www.readbyqxmd.com/read/29240719/pericardial-effusion-as-a-presenting-symptom-of-hashimoto-thyroiditis-a-case-report
#16
Alberto Leonardi, Laura Penta, Marta Cofini, Lucia Lanciotti, Nicola Principi, Susanna Esposito
Background: Hashimoto thyroiditis (HT) is the most frequent cause of acquired hypothyroidism in paediatrics. HT is usually diagnosed in older children and adolescents, mainly in females and is rare in infants and toddlers with cardiac involvement, including pericardial effusion, that can be found in 10% to 30% of adult HT cases. In this paper, a child with HT and pericardial effusion as the most important sign of HT is described. Case presentation: A four-year-old male child suffering for a few months from recurrent abdominal pain sometimes associated with vomiting underwent an abdominal ultrasound scan outside the hospital...
December 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29229564/alternative-myplate-menus-effects-of-ultra-processed-foods-on-saturated-fat-sugar-and-sodium-content
#17
Marilyn Tseng, Dawn B Neill, Stephanie F Teaford, Aydin Nazmi
OBJECTIVE: Compare saturated fat, sugar, and sodium in the US Department of Agriculture sample menu with a version in which menu items were replaced with comparable ultra-processed foods. DESIGN AND SETTING: The authors modified 7 days of a US Department of Agriculture sample menu providing approximately 2,000 kcal/d by replacing foods with comparable ultra-processed alternatives and then visited 2 chain supermarkets in San Luis Obispo, CA (August, 2016) to locate shopping list items for the 2 menu versions...
December 8, 2017: Journal of Nutrition Education and Behavior
https://www.readbyqxmd.com/read/29226646/comparison-of-citrate-buffer-with-sodium-fluoride-as-additives-in-determining-glycemia
#18
Maria de Toro-Crespo, Jose M Maesa-Marquez, Victor Sanchez-Margalet, Concepcion Gonzalez-Rodriguez, Patricia Fernandez-Riejos
BACKGROUND: The objectives of this study are to compare the effect of sodium fluoride and citrate on the stability of glucose in samples maintained at room temperature up to three hours, and to assess the clinical impact in the O'Sullivan test results after changing the additives in the collecting tubes. METHODS: The selected population was pregnant women between the 24th and 28th week of gestation, who were at the health center to undergo the O'Sullivan test as part of the screening program for GDM (gestacional diabetes mellitus)...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29223559/assessment-of-the-stability-of-sheep-sausages-with-the-addition-of-different-concentrations-of-origanum-vulgare-extract-during-storage
#19
R P P Fernandes, M A Trindade, J M Lorenzo, M P de Melo
The aim of this study was to assess the stability of sheep sausages with the addition of different concentrations of Origanum vulgare extract during storage. Five treatments were prepared: without natural antioxidant (control), sodium erythorbate 500mg/kg (ER), and three amounts of extract (N1=4964.51mg/kg, N2=6630.98mg/kg and N3=8038.20mg/kg). From appearance sensory analysis, control treatment differed significantly compared to ER (P<0.05) and N3 (P<0.01) groups, with intense red color, agreeing with trend of a* values...
November 15, 2017: Meat Science
https://www.readbyqxmd.com/read/29222390/sodium-channel-remodeling-in-subcellular-microdomains-of-murine-failing-cardiomyocytes
#20
Mathilde R Rivaud, Esperanza Agullo-Pascual, Xianming Lin, Alejandra Leo-Macias, Mingliang Zhang, Eli Rothenberg, Connie R Bezzina, Mario Delmar, Carol Ann Remme
BACKGROUND: Cardiac sodium channel (NaV1.5) dysfunction contributes to arrhythmogenesis during pathophysiological conditions. Nav1.5 localizes to distinct subcellular microdomains within the cardiomyocyte, where it associates with region-specific proteins, yielding complexes whose function is location specific. We herein investigated sodium channel remodeling within distinct cardiomyocyte microdomains during heart failure. METHODS AND RESULTS: Mice were subjected to 6 weeks of transverse aortic constriction (TAC; n=32) to induce heart failure...
December 8, 2017: Journal of the American Heart Association
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