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https://www.readbyqxmd.com/read/28225559/effects-of-an-alternative-oxidase-gene-on-conidia-viability-under-external-stresses-in-monascus-ruber-m7
#1
Yanchun Shao, Qi Li, Youxiang Zhou, Fusheng Chen
Monascus species can produce natural edible pigments and many other bioactive metabolites. In this study, mraox gene (Monascus ruber alternative oxidase) was isolated, sequenced, and replaced in order to investigate the function in resistance of conidia to stressful conditions. The derived protein of the mraox gene consisted of 350 amino acids with a conserved ferritin-like diiron-binding domain at the C-terminus, sharing a high homolog with alternative oxidase proteins in other filamentous fungi. Deletion of mraox gene repressed the conidia germination rate (CGR) when conidia were exposed to H2 O2 , high temperature (40 and 50 °C) and alkerline buffer (pH8...
February 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28217115/comparison-between-flow-cytometry-and-traditional-culture-methods-for-efficacy-assessment-of-six-disinfectant-agents-against-nosocomial-bacterial-species
#2
Richard Massicotte, Akier A Mafu, Darakhshan Ahmad, Francis Deshaies, Gilbert Pichette, Pierre Belhumeur
The present study was undertaken to compare the use of flow cytometry (FCM) and traditional culture methods for efficacy assessment of six disinfectants used in Quebec hospitals including: two quaternary ammonium-based, two activated hydrogen peroxide-based, one phenol-based, and one sodium hypochlorite-based. Four nosocomial bacterial species, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Vancomycin-resistant Enterococci faecalis, were exposed to minimum lethal concentrations (MLCs) and sublethal concentrations (1/2 MLCs) of disinfectants under study...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28215492/dietary-intake-nutritional-status-and-body-composition-in-children-with-end-stage-kidney-disease-on-hemodialysis-or-peritoneal-dialysis
#3
Consuelo Pontón-Vázquez, Edgar Manuel Vásquez-Garibay, Erika Fabiola Hurtado-López, Adriana de la Torre Serrano, Germán Patiño García, Enrique Romero-Velarde
OBJECTIVE(S): This study aimed to demonstrate that dietary intake, anthropometric indicators, and body composition in children with end-stage kidney disease differs between those on peritoneal dialysis (PD) and those on hemodialysis (HD). METHODS: This was a cross-sectional and consecutive study that included 55 children and adolescents with end-stage kidney disease who were undergoing replacement therapy (22 PD patients and 33 HD patients). Two 24-hour dietary recall surveys were conducted for each patient...
February 16, 2017: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/28213519/an-extra-amino-acid-residue-in-transmembrane-domain-10-of-the-gaba-transporter-gat-1-is-required-for-efficient-ion-coupled-transport
#4
Oshrat Dayan, Anu Nagarajan, Raven Shah, Assaf Ben-Yona, Lucy R Forrest, Baruch I Kanner
The GABA transporter GAT-1 mediates electrogenic transport of its substrate together with sodium and chloride. It is a member of the neurotransmitter:sodium:symporters, which are crucial for synaptic transmission. Compared to all other neurotransmitter:sodium:symporters, GAT-1 and the other members of the GABA transporter subfamily, all contain an extra amino acid residue at or near a conserved glycine in transmembrane segment 10. Therefore, we studied the functional impact of deletion and replacement mutants of Gly-457, and its two adjacent residues in GAT-1...
February 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28213148/interface-tuning-and-stabilization-of-monoglyceride-mesophase-dispersions-food-emulsifiers-and-mixtures-efficiency
#5
Sébastien Serieye, Fabienne Méducin, Irena Milošević, Ling Fu, Samuel Guillot
Several food surfactants were examined as possible efficient emulsifiers for liquid crystalline monolinolein-based particles and as alternative choices to the non-food-grade emulsifier conventionally used Pluronic® F127. We described a food emulsifiers' toolbox, investigating their ability to emulsify mesophases (stabilization capacity, particle size, zeta potential) and their impact on internal nanostructures (from swelling to drastic modifications). Among the selected surfactants, sucrose stearate (S1670) was found to be the best candidate for replacing in a long term F127 as an efficient stabilizer of lipid particles...
January 27, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28196859/dietary-nitrite-reverses-features-of-postmenopausal-metabolic-syndrome-induced-by-high-fat-diet-and-ovariectomy-in-mice
#6
Kazuo Ohtake, Nobuyuki Ehara, Hiroshige Chiba, Genya Nakano, Kunihiro Sonoda, Junta Ito, Hiroyuki Uchida, Jun Kobayashi
Menopausal women are at greater risk of developing metabolic syndrome with reduced endothelial nitric oxide synthase (eNOS) activity. Hormone replacement therapy increases eNOS activity and normalizes some characteristics of metabolic syndrome. We hypothesized that nitric oxide (NO) supplementation should have a therapeutic effect in this syndrome. We examined the effect of dietary nitrite on mice model with postmenopausal metabolic syndrome induced by ovariectomy (OVX) with high fat diet (HF). C57BL/6 female mice were divided into five groups, sham+normal fat diet (NF), sham+ HF, OVX+HF without or with sodium nitrite (50mg and 150mg/L) in drinking water...
February 14, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28193308/review-exogenous-butyrate-implications-for-the-functional-development-of-ruminal-epithelium-and-calf-performance
#7
B Niwińska, E Hanczakowska, M B Arciszewski, R Klebaniuk
The importance of the use of exogenous butyrate in calves' diets is due to its role as a factor stimulating the functional development of ruminal epithelium and improving calf performance during the transition from preruminant to ruminant status. Our review will first present results related to effects of the administration of butyrate in calves' diets on the development of ruminal epithelium toward a more effective absorption and metabolism of fermentation products from the rumen. The introduction of sodium butyrate at a level of about 0...
February 14, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28190351/insights-into-cryoprotective-roles-of-carrageenan-oligosaccharides-in-peeled-whiteleg-shrimp-litopenaeus-vannamei-during-frozen-storage
#8
Bin Zhang, Hui-Cheng Yang, He Tang, Gui-Juan Hao, Yang-Yang Zhang, Shang-Gui Deng
The cryoprotective effects of carrageenan oligosaccharides on peeled whiteleg shrimp were investigated and compared with sodium pyrophosphate treatment during frozen storage, primarily the interaction mechanisms between oligosaccharides and shrimp myosin. Data revealed significant profitable effects on water-holding capacity and textural variables in oligosaccharide-treated shrimp compared to the control. Chemical analyses showed that these saccharides maintained a higher myofibrillar protein content and Ca2+-ATPase activity in frozen shrimp...
February 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28183452/controversies-in-the-management-of-hyperglycaemic-emergencies-in-adults-with-diabetes
#9
REVIEW
Luís Cardoso, Nuno Vicente, Dírcea Rodrigues, Leonor Gomes, Francisco Carrilho
Hyperglycaemic emergencies are associated with significant morbi-mortality and healthcare costs. Management consists on fluid replacement, insulin therapy, and electrolyte correction. However, some areas of patient management remain debatable. In patients without respiratory failure or haemodynamic instability, arterial and venous pH and bicarbonate measurements are comparable. Fluid choice varies upon replenishment phase and patient's condition. If patient is severely hypovolaemic, normal saline solution should be the first option...
March 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28182630/traffic-light-labels-could-reduce-population-intakes-of-calories-total-fat-saturated-fat-and-sodium
#10
Teri E Emrich, Ying Qi, Wendy Y Lou, Mary R L'Abbe
: Traffic-light labelling has been proposed as a public health intervention to improve the dietary intakes of consumers. OBJECTIVES: to model the potential impact of avoiding foods with red traffic lights on the label on the energy, total fat, saturated fat, sodium, and sugars intakes of Canadian adults. METHODS: Canadian adults aged 19 and older (n = 19,915) who responded to the Canadian Community Health Survey (CCHS), Cycle 2.2. The nutrient levels in foods consumed by Canadians in CCHS were profiled using the United Kingdom's criteria for traffic light labelling...
2017: PloS One
https://www.readbyqxmd.com/read/28181941/sodium-bicarbonate-versus-sodium-chloride-for-preventing-contrast-associated-acute-kidney-injury-in-critically-ill-patients-a-randomized-controlled-trial
#11
Xavier Valette, Isabelle Desmeulles, Benoit Savary, Romain Masson, Amélie Seguin, Bertrand Sauneuf, Jennifer Brunet, Pierre Verrier, Véronique Pottier, Marie Orabona, Désiré Samba, Gérald Viquesnel, Mathilde Lermuzeaux, Pascal Hazera, Jean-Jacques Dutheil, Jean-Luc Hanouz, Jean-Jacques Parienti, Damien du Cheyron
OBJECTIVES: To test whether hydration with bicarbonate rather than isotonic sodium chloride reduces the risk of contrast-associated acute kidney injury in critically ill patients. DESIGN: Prospective, double-blind, multicenter, randomized controlled study. SETTING: Three French ICUs. PATIENTS: Critically ill patients with stable renal function (n = 307) who received intravascular contrast media. INTERVENTIONS: Hydration with 0...
February 7, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28178636/comparison-of-three-corrosion-inhibitors-in-simulated-partial-lead-service-line-replacements
#12
Aki Kogo, Sarah Jane Payne, Robert C Andrews
Partial lead service line replacements (PLSLR) were simulated using five recirculating pipe loops treated with either zinc orthophosphate (1mg/L as P), orthophosphate (1mg/L as P) or sodium silicate (10mg/L). Two pipe loops served as "inhibitor-free" (Pb-Cu) and "galvanic free" (Pb-PVC) controls. Changes in water quality (CSMR [0.2 or 1], conductivity [≈330mS/cm or ≈560mS/cm], chlorine [1.4mg/L]) were not observed to provide a significant impact on lead or copper release, although galvanic corrosion was shown to be a driving factor...
January 23, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28162195/-comparison-of-the-effects-of-0-1-bromfenac-sodium-eye-drops-and-corticosteroids-after-small-incision-lenticule-extraction
#13
Y Jiang, Y Li, Y M Jin, J Y Zhang
Objective: To study the safety, efficacy and tolerability of the usage of 0.1% bromfenac sodium eye drops in small incision lenticule extraction (SMILE). Methods: Prospective case control study. Three groups were observed, including 60 patients (60 eyes) undergoing SMILE for myopia. After surgery, 20 patients (20 eyes) were treated with 0.1% bromfenac sodium eye drops twice daily for 10 days, 20 patients (20 eyes) were treated with topical compound tobramycin eye drops 4 times daily for 10 days, and 20 patients (20 eyes) were treated with topical compound tobramycin eye drops 4 times daily for 3 days and 0...
January 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28160775/craniopharyngioma-presenting-with-severe-hyponatremia-hyponatremia-induced-myopathy-and-panhypopituitarism-a-case-report
#14
M D S A Dilrukshi, G V N Sandakumari, P K Abeysundara, T Chang
BACKGROUND: Craniopharyngiomas are rare intracranial tumors commonly presenting with neurological symptoms. Reports of severe hyponatremia as a presenting manifestation of a craniopharyngioma and hyponatremia-induced myopathy are rare. We report the case of a patient with craniopharyngioma presenting with severe hyponatremia, panhypopituitarism, and hyponatremia-induced myopathy. CASE PRESENTATION: A 52-year-old Sri Lankan man presented with anorexia, nausea, fatigue, generalized muscle weakness, and cramps for 1 week...
February 5, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28155851/robust-tissue-growth-and-angiogenesis-in-large-sized-scaffold-by-reducing-h2o2-mediated-oxidative-stress
#15
Girdhari Rijal, Byoung Soo Kim, Falguni Pati, Dong-Heon Ha, Sung Won Kim, Dong-Woo Cho
The implantation of cell-seeded large-sized scaffold often results in insufficient tissue regeneration, which is still a challenge for successful grafting. Excess hydrogen peroxide (H2O2) released by cells propagates oxidative stress, which is the primary cause of tissue injury leading to failure in tissue regeneration. Hence, preventing tissue from oxidative damage becomes imperative. For the first time, we entrapped catalase, an antioxidant in a scaffold as a novel approach in bioengineering to prevent tissue from H2O2-induced damage...
February 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28154142/dissecting-the-proton-transport-pathway-in-electrogenic-na-h-antiporters
#16
Povilas Uzdavinys, Mathieu Coinçon, Emmanuel Nji, Mama Ndi, Iven Winkelmann, Christoph von Ballmoos, David Drew
Sodium/proton exchangers of the SLC9 family mediate the transport of protons in exchange for sodium to help regulate intracellular pH, sodium levels, and cell volume. In electrogenic Na(+)/H(+) antiporters, it has been assumed that two ion-binding aspartate residues transport the two protons that are later exchanged for one sodium ion. However, here we show that we can switch the antiport activity of the bacterial Na(+)/H(+) antiporter NapA from being electrogenic to electroneutral by the mutation of a single lysine residue (K305)...
February 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28148262/demographic-factors-associated-with-dietary-supplement-prescriptions-filled-by-united-states-military-service-members-2005-2013
#17
Joseph J Knapik, Rosenie T Jean, Krista G Austin, Ryan A Steelman, Emily K Farina, Harris R Lieberman
BACKGROUND: Dietary supplements (DSs) can be purchased over-the-counter but may also be prescribed by medical personnel for specific therapeutic reasons. Few studies have examined this latter source of DSs despite the fact that 79% of physicians and 82% of nurses have recommended DSs to their patients. This investigation examined demographic factors associated with temporal trends in oral DS prescriptions filled by all United States (US) service members (SMs) from 2005 to 2013 (n = 1,427,080 ± 22,139, mean ± standard deviation per year)...
January 31, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28139287/depletion-rate-of-hydrogen-peroxide-from-sodium-perborate-bleaching-agent
#18
Liliann Tran, Rebecca Orth, Peter Parashos, Ying Tao, Calvin W J Tee, Vineet Thenalil Thomas, Georgina Towers, Diem Thuy Truong, Cynthia Vinen, Eric C Reynolds
INTRODUCTION: Internal bleaching of discolored teeth uses sodium perborate reacting with water to form the active agent, hydrogen peroxide (H2O2). Sodium perborate is replaced at varying time intervals depending on clinician preference and until esthetically acceptable results are achieved, but this is done without scientific basis. This study measured the depletion rate of hydrogen peroxide from sodium perborate as a bleaching agent. METHODS: Two sodium perborate bleaching products (Odontobleach [Australian Dental Manufacturing, Kenmore Hills, Queensland, Australia] and Endosure Perborate Micro [Dentalife, Ringwood, Victoria, Australia]) and distilled deionized water mixtures at ratios of 25 μg/mL, 50 μg/mL, and 100 μg/mL were placed into sealed microtubes and incubated at 37°C...
January 27, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28134948/synthesis-and-characterisation-of-tetranuclear-ruthenium-polyhydrido-clusters-with-pseudo-tetrahedral-geometry
#19
Hajime Kameo, Yutaka Ito, Ryuichi Shimogawa, Asuka Koizumi, Hiroki Chikamori, Junko Fujimoto, Hiroharu Suzuki, Toshiro Takao
Dicationic tetranuclear ruthenium octahydride [(Cp*Ru)4H8](2+) (5) with tetrahedral geometry was obtained by reaction of dinuclear ruthenium tetrahydride (Cp*Ru)2(μ-H)4 (1) with an excess of Brønsted acids, such as HBF4·OEt2, in toluene. Monocationic tetraruthenium heptahydride [(Cp*Ru)4H7](+) (7) was obtained by dropwise addition of a diluted acid to a rigorously stirred solution of 1 at ambient temperature. Dication 5 was converted into monocationic heptahydrido complex 7 in high yield by treatment with sodium methoxide or sodium hydride...
January 30, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28132947/an-addison-disease-revealed-with-a-serious-hyponatremia
#20
Hadrien Maguet, Agnès Carreau, Serge Hautefeuille, Pierre Bonnin, Gaspard Beaune
We present the case of an Addison's disease revealed by a serious hyponatremia. The serum concentration of ACTH and 21-hydroxylase antibodies were increased and lead to the diagnosis. The cortisol blood level was lowered but required to take into account the stress induced by the hospitalisation of the patient. Addison's disease is characterized by the destruction of the adrenal cortex. Autoimmune adrenalitis is the main cause of adrenal insufficiency. Treatment involves normalisation of sodium concentration and corticosteroids replacement...
February 1, 2017: Annales de Biologie Clinique
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