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https://www.readbyqxmd.com/read/28239868/determination-of-two-potential-toxicity-metabolites-derived-from-the-disruption-of-pksct-gene-in-monascus-aurantiacus-li-as3-4384
#1
Zhibing Huang, Baowei Su, Yang Xu, Laisheng Li, Yanping Li
BACKGROUND: We previously demonstrated that disruption of the pksCT gene of Monascus led to over 98% decrease in its citrinin production capacity in Monascus (PHDS26).Two potentially toxic compounds, monascopyridine A (MPA) and monascopyridine B (MPB), were found in the fermentation products of the pksCT gene-disrupted Monascus. Moreover, a rapid and reliable HPLC method was developed for the simultaneous determination of MPA and MPB. We studied the effects of various extraction parameters and designed an orthogonal experiment to investigate the importance of each factor...
February 27, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28239841/the-structure-of-nursing-a-national-examination-of-titles-and-practice-profiles
#2
G Gardner, C Duffield, A Doubrovsky, U T Bui, M Adams
AIM: To identify the practice patterns of the Australian registered nurse workforce according to position title and to map these disparate titles across all jurisdictions of the country. INTRODUCTION: Effective nursing workforce planning can contribute to improved patient care and increased effectiveness and efficiency of healthcare systems. BACKGROUND: The prevailing approach to nursing workforce planning focuses on number of nursing staff needed in relation to the number of patients...
February 26, 2017: International Nursing Review
https://www.readbyqxmd.com/read/28239779/variation-in-barley-1%C3%A2-%C3%A2-3-1%C3%A2-%C3%A2-%C3%A2-4-%C3%AE-glucan-endohydrolases-reveals-novel-allozymes-with-increased-thermostability
#3
Juanita C Lauer, Suong Cu, Rachel A Burton, Jason K Eglinton
Novel barley (1 → 3, 1 → 4)-β-glucan endohydrolases with increased thermostability. Rapid and reliable degradation of (1 → 3, 1 → 4)-β-glucan to produce low viscosity wort is an essential requirement for malting barley. The (1 → 3, 1 → 4)-β-glucan endohyrolases are responsible for the primary hydrolysis of cell wall β-glucan. The variation in β-glucanase genes HvGlb1 and HvGlb2 that encode EI and EII, respectively, were examined in elite and exotic germplasm. Six EI and 14 EII allozymes were identified, and significant variation was found in β-glucanase from Hordeum vulgare ssp...
February 26, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28239729/a-pure-dna-hydrogel-with-stable-catalytic-ability-produced-by-one-step-rolling-circle-amplification
#4
Yishun Huang, Wanlin Xu, Guoyuan Liu, Leilei Tian
A rolling-circle-amplification method was developed to produce DNA hydrogels with horseradish-peroxidase-like catalytic capability. The catalytic hydrogel exhibits highly improved stability at elevated temperatures or during a long-term storage. Integrated with glucose oxidase, the complex hydrogel can be applied to the sensitive and reliable detection of glucose.
February 27, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28239666/making-the-most-of-rna-seq-pre-processing-sequencing-data-with-opossum-for-reliable-snp-variant-detection
#5
Laura Oikkonen, Stefano Lise
Identifying variants from RNA-seq (transcriptome sequencing) data is a cost-effective and versatile alternative to whole-genome sequencing. However, current variant callers do not generally behave well with RNA-seq data due to reads encompassing intronic regions. We have developed a software programme called Opossum to address this problem. Opossum pre-processes RNA-seq reads prior to variant calling, and although it has been designed to work specifically with Platypus, it can be used equally well with other variant callers such as GATK HaplotypeCaller...
January 17, 2017: Wellcome Open Res
https://www.readbyqxmd.com/read/28239663/radiographic-indices-for-lumbar-developmental-spinal-stenosis
#6
Jason Pui Yin Cheung, Karen Ka Man Ng, Prudence Wing Hang Cheung, Dino Samartzis, Kenneth Man Chee Cheung
BACKGROUND: Patients with developmental spinal stenosis (DSS) are susceptible to developing symptomatic stenosis due to pre-existing narrowed spinal canals. DSS has been previously defined by MRI via the axial anteroposterior (AP) bony spinal canal diameter. However, MRI is hardly a cost-efficient tool for screening patients. X-rays are superior due to its availability and cost, but currently, there is no definition of DSS based on plain radiographs. Thus, the aim of this study is to develop radiographic indices for diagnosing DSS...
2017: Scoliosis and Spinal Disorders
https://www.readbyqxmd.com/read/28239651/profiling-of-circulating-micrornas-in-children-with-recent-onset-of-type-1-diabetes
#7
Suheda Erener, Ashish Marwaha, Rusung Tan, Constadina Panagiotopoulos, Timothy J Kieffer
Type 1 diabetes (T1D) is an autoimmune disease that is clinically silent until the majority of β cells are destroyed. There is an unmet need for reliable and cost-effective biomarkers to predict and diagnose diabetes at an early stage. A number of stable microRNAs (miRNAs) have been reported in serum and plasma and are now being investigated as biomarkers of different diseases. We measured the levels of 745 miRNAs in sera of children with recent-onset T1D and age-matched controls using locked nucleic acid-enhanced (LNA-enhanced) quantitative PCR profiling...
February 23, 2017: JCI Insight
https://www.readbyqxmd.com/read/28239615/apomorphine-subcutaneous-injection-for-the-management-of-morning-akinesia-in-parkinson-s-disease
#8
Stuart Isaacson, Mark Lew, William Ondo, Jean Hubble, Thomas Clinch, Fernando Pagan
BACKGROUND: In patients with motor fluctuations complicating Parkinson's disease (PD), delays in time-to-ON with levodopa are common. This open-label study aimed to assess the effect of apomorphine on time-to-ON in PD patients with morning akinesia. METHODS: The safety population included 127 enrolled patients, and the full analysis set (FAS) included 88 patients. Patients completed a 7-day levodopa baseline period recording their time-to-ON following each morning dose of levodopa...
January 2017: Movement Disorders Clinical Practice
https://www.readbyqxmd.com/read/28239573/role-of-buccal-pad-of-fat-in-reconstruction-of-the-buccal-mucosa-defects
#9
A Sagayaraj, N Divya Jyothi, S M Azeem Mohiyuddin, Ravindra P Deo, B Vageesh Padiyar
Carcinoma buccal mucosa is the most common oral cavity cancer in India. Following excision of these lesions the defects can be reconstructed using various reconstructive techniques. Buccal pad of fat has been successfully used in the reconstruction of small palatal defects and in the closure of the oro antral fistula. This study aims at evaluating the role of buccal pad of fat in reconstruction of defects following excision of the small to medium premalignant lesions and T1-T2 malignant lesions of buccal mucosa...
March 2017: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/28239549/red-legged-partridge-alectoris-rufa-de-novo-transcriptome-assembly-and-identification-of-gene-related-markers
#10
Natalia Sevane, Javier Cañon, Paulina G Eusebi, Ignacio Gil, Susana Dunner
The red-legged partridge (Alectoris rufa) has a great socio-economic importance as a game species and is reared by millions in farms in several European countries. The ability to respond to a wide spectrum of pathogens and environmental changes is key for farm-reared animals that, as such, face even higher pathogen exposure and specifically for those submitted to restocking programs. In this study, RNA-sequencing and de-novo assembly of genes expressed in different immune tissues were performed. The raw FASTQ files were submitted to the NCBI SRA database with accession number PRJNA289204...
March 2017: Genomics Data
https://www.readbyqxmd.com/read/28239525/reproducibility-of-tract-based-white-matter-microstructural-measures-using-the-enigma-dti-protocol
#11
Ashley Acheson, S Andrea Wijtenburg, Laura M Rowland, Anderson Winkler, Charles W Mathias, L Elliot Hong, Neda Jahanshad, Binish Patel, Paul M Thompson, Stephen A McGuire, Paul M Sherman, Peter Kochunov, Donald M Dougherty
BACKGROUND: In preparation for longitudinal analyses of white matter development in youths with family histories of substance use disorders (FH+) or without such histories (FH-), we examined the reproducibility and reliability of global and regional measures of fractional anisotropy (FA) values, measured using the Enhancing Neuro Imaging Genetics Through Meta Analysis (ENIGMA)-diffusion tensor imaging (DTI) protocol. Highly reliable measures are necessary to detect any subtle differences in brain development...
February 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28239434/a-hybrid-causal-search-algorithm-for-latent-variable-models
#12
Juan Miguel Ogarrio, Peter Spirtes, Joe Ramsey
Existing score-based causal model search algorithms such as GES (and a speeded up version, FGS) are asymptotically correct, fast, and reliable, but make the unrealistic assumption that the true causal graph does not contain any unmeasured confounders. There are several constraint-based causal search algorithms (e.g RFCI, FCI, or FCI+) that are asymptotically correct without assuming that there are no unmeasured confounders, but often perform poorly on small samples. We describe a combined score and constraint-based algorithm, GFCI, that we prove is asymptotically correct...
August 2016: JMLR Workshop and Conference Proceedings
https://www.readbyqxmd.com/read/28239411/reliable-measurement-of-e-coli-single-cell-fluorescence-distribution-using-a-standard-microscope-set-up
#13
Marilisa Cortesi, Lucia Bandiera, Alice Pasini, Alessandro Bevilacqua, Alessandro Gherardi, Simone Furini, Emanuele Giordano
BACKGROUND: Quantifying gene expression at single cell level is fundamental for the complete characterization of synthetic gene circuits, due to the significant impact of noise and inter-cellular variability on the system's functionality. Commercial set-ups that allow the acquisition of fluorescent signal at single cell level (flow cytometers or quantitative microscopes) are expensive apparatuses that are hardly affordable by small laboratories. METHODS: A protocol that makes a standard optical microscope able to acquire quantitative, single cell, fluorescent data from a bacterial population transformed with synthetic gene circuitry is presented...
2017: Journal of Biological Engineering
https://www.readbyqxmd.com/read/28239409/iroa-international-register-of-open-abdomen-preliminary-results
#14
Federico Coccolini, Giulia Montori, Marco Ceresoli, Fausto Catena, Rao Ivatury, Michael Sugrue, Massimo Sartelli, Paola Fugazzola, Davide Corbella, Francesco Salvetti, Ionut Negoi, Monica Zese, Savino Occhionorelli, Stefano Maccatrozzo, Sergei Shlyapnikov, Christian Galatioto, Massimo Chiarugi, Zaza Demetrashvili, Daniele Dondossola, Yovcho Yovtchev, Orestis Ioannidis, Giuseppe Novelli, Mirco Nacoti, Desmond Khor, Kenji Inaba, Demetrios Demetriades, Torsten Kaussen, Asri Che Jusoh, Wagih Ghannam, Boris Sakakushev, Ohad Guetta, Agron Dogjani, Stefano Costa, Sandeep Singh, Dimitrios Damaskos, Arda Isik, Kuo-Ching Yuan, Francesco Trotta, Stefano Rausei, Aleix Martinez-Perez, Giovanni Bellanova, Vinicius Cordeiro Fonseca, Fernando Hernández, Athanasios Marinis, Wellington Fernandes, Martha Quiodettis, Miklosh Bala, Andras Vereczkei, Rafael L Curado, Gustavo Pereira Fraga, Bruno M Pereira, Mahir Gachabayov, Guillermo Perez Chagerben, Miguel Leon Arellano, Sefa Ozyazici, Gianluca Costa, Tugan Tezcaner, Luca Ansaloni
BACKGROUND: No definitive data about open abdomen (OA) epidemiology and outcomes exist. The World Society of Emergency Surgery (WSES) and the Panamerican Trauma Society (PTS) promoted the International Register of Open Abdomen (IROA). METHODS: A prospective observational cohort study including patients with an OA treatment. Data were recorded on a web platform (Clinical Registers®) through a dedicated website: www.clinicalregisters.org. RESULTS: Four hundred two patients enrolled...
2017: World Journal of Emergency Surgery: WJES
https://www.readbyqxmd.com/read/28239407/the-greek-orthodox-version-of-the-brief-religious-coping-b-rcope-instrument-psychometric-properties-in-three-samples-and-associations-with-mental-disorders-suicidality-illness-perceptions-and-quality-of-life
#15
Vassiliki Paika, Elias Andreoulakis, Elisavet Ntountoulaki, Dimitra Papaioannou, Konstantinos Kotsis, Vassiliki Siafaka, Konstantinos N Fountoulakis, Kenneth I Pargament, Andre F Carvalho, Thomas Hyphantis
BACKGROUND: The B-RCOPE is a brief measure assessing religious coping. We aimed to assess the psychometric properties of its Greek version in people with and without long-term conditions (LTCs). Associations between religious coping and mental illness, suicidality, illness perceptions, and quality of life were also investigated. METHODS: The B-RCOPE was administered to 351 patients with diabetes, chronic pulmonary obstructive disease (COPD), and rheumatic diseases attending either the emergency department (N = 74) or specialty clinics (N = 302) and 127 people without LTCs...
2017: Annals of General Psychiatry
https://www.readbyqxmd.com/read/28239394/standardization-and-validation-of-dot-elisa-assay-for-paracoccidioides-brasiliensis-antibody-detection
#16
Camila Mika Kamikawa, Rinaldo Poncio Mendes, Adriana Pardini Vicentini
BACKGROUND: Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28239365/characterizing-early-maternal-style-in-a-population-of-guide-dogs
#17
Emily E Bray, Mary D Sammel, Dorothy L Cheney, James A Serpell, Robert M Seyfarth
In both humans and non-humans, differences in maternal style during the first few weeks of life can be reliably characterized, and these differences affect offspring's temperament and cognition in later life. Drawing on the breeding population of dogs at The Seeing Eye, a guide dog school in Morristown, New Jersey, we conducted videotaped focal follows on 21 mothers and their litters (n = 138 puppies) over the first 3 weeks of the puppies' lives in an effort to characterize maternal style. We found that a mother's attitude and actions toward her offspring varied naturally between individuals, and that these variations could be summarized by a single principal component, which we described as Maternal behavior...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28239215/the-techniques-for-overcoming-depression-questionnaire-mokken-scale-analysis-reliability-and-concurrent-validity-in-depressed-cardiac-patients
#18
Kenneth E Freedland, Mariantonia Lemos, Frank Doyle, Brian C Steinmeyer, Iris Csik, Robert M Carney
OBJECTIVES: The Techniques for Overcoming Depression (TOD) questionnaire assesses the frequency with which patients being treated for depression use cognitive-behavioral techniques in daily life. This study examined its latent structure, reliability and concurrent validity in depressed cardiac patients. METHOD: The TOD was administered at the initial and final treatment sessions in three trials of cognitive behavior therapy (CBT) (n = 260) for depression in cardiac patients...
February 2017: Cognitive Therapy and Research
https://www.readbyqxmd.com/read/28239074/joint-attention-development-in-low-risk-very-low-birth-weight-infants-at-around-18-months-of-age
#19
Noriko Yamaoka, Satoshi Takada
The purpose of this study was to clarify the developmental characteristics of joint attention in very low birth weight (VLBW) infants with a low risk of complications. Section B of the Checklist for Autism in Toddlers (CHAT) was administered to 31 VLBW and 45 normal birth weight (NBW) infants aged 18-22 months, while the sessions were recorded with a video camera. A semi-structured observation scale was developed to assess infants' joint attention from the video footage, and was shown to be reliable. VLBW, compared to NBW, infants showed significantly poorer skills in 2 of 4 items on responding to joint attention, and in 6 of 10 items on initiating joint attention...
October 18, 2016: Kobe Journal of Medical Sciences
https://www.readbyqxmd.com/read/28238944/brain-correlates-of-hypnosis-a-systematic-review-and-meta-analytic-exploration
#20
REVIEW
Mathieu Landry, Michael Lifshitz, Amir Raz
Imaging of the living human brain elucidates the neural dynamics of hypnosis; however, few reliable brain patterns emerge across studies. Here, we methodically assess neuroimaging assays of hypnosis to uncover common neural configurations using a twofold approach. First, we systematically review research on the neural correlates of hypnotic phenomena; then, we meta-analyze these collective data seeking specific activation and deactivation patterns that typify hypnosis. Anchored around the role of top-down control processes, our comprehensive examination focuses on the involvement of intrinsic brain networks known to operationalize cognitive control and self-referential cognition, including the executive, salience, and default networks...
February 23, 2017: Neuroscience and Biobehavioral Reviews
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