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Osteopontin

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https://www.readbyqxmd.com/read/28453726/osteopontin-rna-aptamer-can-prevent-and-reverse-pressure-overload-induced-heart-failure
#1
Jihe Li, Keyvan Yousefi, Wen Ding, Jayanti Singh, Lina A Shehadeh
Aims: Cardiac myocyte hypertrophy, the main compensatory response to chronic stress in the heart often progresses to a state of decompensation that can lead to heart failure. Osteopontin (OPN) is an effector for extracellular signalling that induces myocyte growth and fibrosis. Although increased OPN activity has been observed in stressed myocytes and fibroblasts, the detailed and long term effects of blocking OPN signalling on the heart remain poorly defined. Targeting cardiac OPN protein by an RNA aptamer may be beneficial for tuning down OPN pathologic signalling...
May 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28446370/microcarrier-culture-enhances-osteogenic-potential-of-human-periodontal-ligament-stromal-cells
#2
Alina Čebatariūnienė, Akvilė Jarmalavičiūtė, Virginijus Tunaitis, Alina Pūrienė, Algirdas Venalis, Augustas Pivoriūnas
Regeneration of periodontal tissue represents a major challenge to modern tissue engineering, since cell-based therapies require large amounts of periodontal ligament stromal cells (PLSC), which can be obtained only by in vitro expansion. Ideally, the period of the in vitro expansion should be optimized for the generation of large enough numbers of pre-specified progenitor cells ready to contribute to the restoration of periodontal tissues. In the present study, we used a commercially available, three-dimensional culturing platform and alginate microcarrier cell culture system for the propagation of human PLSCs, which were derived using the explant outgrowth method...
March 29, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28445750/effect-of-phosphorylation-on-a-human-like-osteopontin-peptide
#3
Samuel Lenton, Marco Grimaldo, Felix Roosen-Runge, Frank Schreiber, Tommy Nylander, Roger Clegg, Carl Holt, Michael Härtlein, Victoria García Sakai, Tilo Seydel, Susana C Marujo Teixeira
The last decade established that the dynamic properties of the phosphoproteome are central to function and its modulation. The temporal dimension of phosphorylation effects remains nonetheless poorly understood, particularly for intrinsically disordered proteins. Osteopontin, selected for this study due to its key role in biomineralization, is expressed in many species and tissues to play a range of distinct roles. A notable property of highly phosphorylated isoforms of osteopontin is their ability to sequester nanoclusters of calcium phosphate to form a core-shell structure, in a fluid that is supersaturated but stable...
April 25, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28445262/dose-dependence-of-pth-related-peptide-1-on-the-osteogenic-induction-of-mc3t3-e1-cells-in-vitro
#4
Jianping Wang, Jingfeng Li, Liang Yang, Yichi Zhou, Yi Wang
Parathyroid hormone (PTH), an 84-amino acid peptide, is an endocrine hormone that is secreted by parathyroid glands. PTH performs important functions in calcium regulation and bone remodeling. The PTH (1-34) named teriparatide, a 34-amino acid peptide derived from the N-terminus of PTH, conserves most of the functions of PTH, specifically the osteogenic capability. However, teriparatide is only used by injection and exhibits short duration. In addition, this PTH could not thoroughly expose active sites. In this study, a novel PTH-related peptide (designated PTHrP-1) derived from the N-terminus of PTH was added into the complete medium at different concentrations of PTHrP-1 (0, 50, 100, and 200 ng/mL) to induce the MC3T3-E1 cells...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28444839/age-dependent-alterations-in-osteoblast-and-osteoclast-activity-in-human-cancellous-bone
#5
Mustafa Becerikli, Henriette Jaurich, Jessica Schira, Matthias Schulte, Carmen Döbele, Christoph Wallner, Stephanie Abraham, Johannes M Wagner, Mehran Dadras, Ulrich Kneser, Marcus Lehnhardt, Björn Behr
It is assumed that the activity of osteoblasts and osteoclasts is decreased in bone tissue of aged individuals. However, detailed investigation of the molecular signature of human bone from young compared to aged individuals confirming this assumption is lacking. In this study, quantitative expression analysis of genes related to osteogenesis and osteoclastogenesis of human cancellous bone derived from the distal radius of young and aged individuals was performed. Furthermore, we additionally performed immunohistochemical stainings...
April 26, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28442663/histochemical-assessment-for-osteoblastic-activity-coupled-with-dysfunctional-osteoclasts-in-c-src-deficient-mice
#6
Hisashi Toray, Tomoka Hasegawa, Naoko Sakagami, Erika Tsuchiya, Ai Kudo, Shen Zhao, Yasuhito Moritani, Miki Abe, Taiji Yoshida, Tomomaya Yamamoto, Tsuneyuki Yamamoto, Kimimitsu Oda, Nobuyuki Udagawa, Paulo Henrique Luiz de Freitas, Minqi Li
Since osteoblastic activities are believed to be coupled with osteoclasts, we have attempted to histologically verify which of the distinct cellular circumstances, the presence of osteoclasts themselves or bone resorption by osteoclasts, is essential for coupled osteoblastic activity, by examining c-fos(-/-) or c-src(-/-) mice. Osteopetrotic c-fos deficient (c-fos(-/-)) mice have no osteoclasts, while c-src deficient (c-src(-/-)) mice, another osteopetrotic model, develop dysfunctional osteoclasts due to a lack of ruffled borders...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28442375/implication-of-hyperoxaluria-on-osteopontin-and-er-stress-mediated-apoptosis-in-renal-tissue-of-rats
#7
Rishi Bhardwaj, Ankita Bhardwaj, Chanderdeep Tandon, Devinder K Dhawan, Rakesh Kumar Bijarnia, Tanzeer Kaur
Hyperoxaluria is a stress that leads to calcium oxalate crystal deposition which further causes inflammation and renal cell necroptosis. Many studies have linked osteopontin expression with apoptosis and inflammation but so far its association with apoptosis with regard to hyperoxaluria is undiscovered. Moreover, a recent report has suggested that osteopontin induces endoplasmic reticulum stress and subsequently apoptosis in myocytes. In this study, the impact of hyperoxaluria on the modulation of osteopontin expression and endoplasmic reticulum (ER) stress mediated apoptosis in rats is explored...
April 22, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28441755/role-of-rheb-in-regulating-differentiation-fate-of-mesenchymal-stem-cells-for-cartilage-and-bone-regeneration
#8
Sajjad Ashraf, In-Bo Han, Hansoo Park, Soo-Hong Lee
Advances in mesenchymal stem cells (MSCs) and cell replacement therapies are promising approaches to treat cartilage and bone defects since substantial differentiation capacities of MSCs match the demands of tissue regeneration. Our understanding of the dynamic process requiring indispensable differentiation of MSCs remains limited. Herein, we describe the role of RHEB (Ras homolog enriched in brain) regulating gene signature for differentiation of human adipose derived mesenchymal stem cells (ASCs) into chondrogenic, osteogenic, and adipogenic lineages...
April 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28440483/human-osteopontin-potential-clinical-applications-in-cancer-review
#9
Chengcheng Hao, Yuxin Cui, Sionen Owen, Wenbin Li, Shan Cheng, Wen G Jiang
Human osteopontin (OPN) is a glycosylated phosphoprotein which is expressed in a variety of tissues in the body. In recent years, accumulating evidence has indicated that the aberrant expression of OPN is closely associated with tumourigensis, progression and most prominently with metastasis in several tumour types. In this review, we present the current knowledge on the expression profiles of OPN and its main splice variants in human cancers, as well as the potential implications in patient outcome. We also discuss its putative clinical application as a cancer biomarker and as a therapeutic target...
April 21, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28437177/periodontal-derived-mesenchymal-cell-sheets-promote-periodontal-regeneration-in-inflammatory-microenvironment
#10
Shujuan Guo, Jian Kang, Baohui Ji, Weihua Guo, Yi Ding, Yafei Wu, Weidong Tian
In this study, we investigated the periodontal regenerative potential of dental follicle cell (DFC) sheets and periodontal ligament cell (PDLC) sheets in the simulating inflammatory microenvironment of periodontitis, in order to confirm their regenerative potential for clinical application and explain the possible mechanism. The biological characteristics of DFC sheets and PDLC sheets were explored in lipopolysaccharid from Porphyromonas gingivalis (P. gingivalis LPS) induced inflammation microenvironment in vitro, then cell sheets were transplanted into canine periodontal defects with experimental periodontitis in situ for three months...
February 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28435843/mmp-7-is-a-predictive-biomarker-of-disease-progression-in-patients-with-idiopathic-pulmonary-fibrosis
#11
Yasmina Bauer, Eric S White, Simon de Bernard, Peter Cornelisse, Isabelle Leconte, Adele Morganti, Sebastien Roux, Oliver Nayler
Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with poor prognosis, which is characterised by destruction of normal lung architecture and excessive deposition of lung extracellular matrix. The heterogeneity of disease progression in patients with IPF poses significant obstacles to patient care and prevents efficient development of novel therapeutic interventions. Blood biomarkers, reflecting pathobiological processes in the lung, could provide objective evidence of the underlying disease...
January 2017: ERJ Open Research
https://www.readbyqxmd.com/read/28433634/dual-sphingosine-kinase-inhibitor-ski-ii-enhances-sensitivity-to-5-fluorouracil-in-hepatocellular-carcinoma-cells-via-suppression-of-osteopontin-and-fak-igf-1r-signalling
#12
Petra Grbčić, Ivana Tomljanović, Marko Klobučar, Sandra Kraljević Pavelić, Ksenija Lučin, Mirela Sedić
Hepatocellular carcinoma (HCC) represents the third leading cause of cancer-related deaths globally. Although 5-Fluorouracil (5-FU) is used as the first choice treatment for advanced HCC, it exerts poor efficacy and is associated with acquired and intrinsic resistance. Sphingosine kinases (Sphk) 1 and 2 play tumour-promoting roles in different cancer types including HCC and thus represent promising pharmacological targets. In the present study, we have investigated for the first time the anticancer efficacy and underlying molecular mechanisms of combined administration of 5-FU and dual Sphk1/Sphk2 inhibitor SKI-II (4-[[4-(4-chlorophenyl)-1,3-thiazol-2-yl]amino]phenol) in HepG2 hepatocellular carcinoma cells...
April 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28429412/bone-extracellular-matrix-hydrogel-enhances-osteogenic-differentiation-of-c2c12-myoblasts-and-mouse-primary-calvarial-cells
#13
Noura Alom, Heather Peto, Glen R Kirkham, Kevin M Shakesheff, Lisa J White
Hydrogel scaffolds derived from the extracellular matrix (ECM) of mammalian tissues have been successfully used to promote tissue repair in vitro and in vivo. The objective of this study was to evaluate the osteogenic potential of ECM hydrogels prepared from demineralized and decellularized bovine bone in the presence and absence of osteogenic medium. Culture of C2C12 and mouse primary calvarial cells (mPCs) on decellularized bone ECM (bECM) and demineralized bone matrix (DBM) gels resulted in increased expression of osteogenic gene markers, including a 3...
April 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28428079/mineralization-inhibiting-effects-of-transglutaminase-crosslinked-polymeric-osteopontin
#14
Betty Hoac, Valentin Nelea, Wenge Jiang, Mari T Kaartinen, Marc D McKee
Osteopontin (OPN) belongs to the SIBLING family (Small, Integrin-Binding LIgand N-linked Glycoproteins) of mineral-binding matrix proteins found in bones and teeth. OPN is a well-known inhibitor of matrix mineralization, and enzymatic modification of OPN can affect this inhibitory function. In bone, OPN exists both as a monomer and as a high-molecular-weight polymer - the latter is formed by transglutaminase-mediated crosslinking of glutamine and lysine residues in OPN to create homotypic protein assemblies...
April 18, 2017: Bone
https://www.readbyqxmd.com/read/28427342/intermittent-parathyroid-hormone-pth-promotes-cementogenesis-and-alleviates-the-catabolic-effects-of-mechanical-strain-in-cementoblasts
#15
Yuyu Li, Zhiai Hu, Chenchen Zhou, Yang Xu, Li Huang, Xin Wang, Shujuan Zou
BACKGROUND: External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment...
April 20, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28423178/head-to-head-comparison-of-structurally-unrelated-dpp4-inhibitors-in-the-setting-of-renal-ischemia-reperfusion-injury
#16
Christoph Reichetzeder, Karoline von Websky, Oleg Tsuprykov, Azadeh Mohagheghi Samarin, Luise Gabriele Falke, Sulistyo Emantoko Dwi Putra, Ahmed Abdallah Hasan, Viktoriia Antonenko, Caterina Curato, Jörg Rippman, Thomas Klein, Berthold Hocher
BACKGROUND AND PURPOSE: Results regarding protective effects of DPP4 inhibitors in renal ischemia-reperfusion-injury (IRI) are conflicting. The current study performed a head-to-head comparison of structurally unrelated DPP4 inhibitors in the setting of renal IRI. EXPERIMENTAL APPROACH: IRI was induced in uninephrectomized male rats by renal artery clamping for 30 minutes. The sham group was uninephrectomized but not subjected to IRI. DPP4 inhibitors or vehicle were given p...
April 18, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28420872/hairy-enhancer-of-split-homologue-1-suppresses-vascular-endothelial-growth-factor-induced-angiogenesis-via-downregulation-of-osteopontin-expression
#17
Xing-Xing Yao, Jing-Bo Lu, Zhi-Dong Ye, Lei Zheng, Qian Wang, Zhi-Qi Lin, Hao Liu, Heng Wan, Fang-Yong Fu, Xian-Ying Huang, Jian-Chen Xiu, Zheng-Jun Liu, Yan-Wei Hu
Angiogenesis plays a critical role in the progression and vulnerability of atherosclerotic plaques; however, the orchestration of angiogenesis in atherosclerotic plaque formation remains unclear. The results of microarray analysis, real-time PCR and immunohistochemical analyses showed that Hairy/enhancer of split homologue-1 (Hes-1) expression was significantly decreased, while that of osteopontin (OPN) was increased, in atherosclerotic plaques. Meanwhile, immunofluorescence results demonstrated that both Hes-1 and OPN were expressed in endothelial cells (ECs) of neovessels in atherosclerotic plaques...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418701/cementogenesis-is-inhibited-under-a-mechanical-static-compressive-force-via-piezo1
#18
Ying-Ying Zhang, Yi-Ping Huang, Hua-Xiang Zhao, Ting Zhang, Feng Chen, Yan Liu
OBJECTIVE: To investigate whether Piezo1, a mechanotransduction gene mediates the cementogenic activity of cementoblasts under a static mechanical compressive force. MATERIALS AND METHODS: Murine cementoblasts (OCCM-30) were exposed to a 2.0 g/cm(2) static compressive force for 3, 6, 12, and 24 hours. Then the expression profile of Piezo1 and the cementogenic activity markers osteoprotegerin (Opg), osteopontin (Opn), osteocalcin (Oc), and protein tyrosine phosphataselike member A (Ptpla) were analyzed...
April 18, 2017: Angle Orthodontist
https://www.readbyqxmd.com/read/28417915/high-glucose-accelerates-cell-proliferation-and-increases-the-secretion-and-mrna-expression-of-osteopontin-in-human-pancreatic-duct-epithelial-cells
#19
Miho Ito, Naohiko Makino, Akiko Matsuda, Yushi Ikeda, Yasuharu Kakizaki, Yoshihiko Saito, Yoshiyuki Ueno, Sumio Kawata
BACKGROUND: The incidence of pancreatic cancer is increasing year-by-year in Japan. Among the diseases that complicate pancreatic cancer, diabetes is the most common. Recently, it has become evident that patients suffering from diabetes and obesity show increased expression of osteopontin (OPN). The purpose of this study was to investigate the effect of high glucose and high insulin culture conditions on a human pancreatic duct epithelial cell line (HPDE-6), focusing particularly on OPN expression...
April 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28415692/novel-akt-activator-sc-79-is-a-potential-treatment-for-alcohol-induced-osteonecrosis-of-the-femoral-head
#20
Yi-Xuan Chen, Shi-Cong Tao, Zheng-Liang Xu, Wen-Jing Yin, Yue-Lei Zhang, Jun-Hui Yin, You-Shui Gao, Chang-Qing Zhang
Alcohol is a leading risk factor for osteonecrosis of the femoral head (ONFH). We explored the molecular mechanisms underlying alcohol-induced ONFH and investigated the protective effect of the novel Akt activator SC-79 against this disease. We found that ethanol inhibited expression of the osteogenic genes RUNX2 and OCN, downregulated osteogenic differentiation, impaired the recruitment of Akt to the plasma membrane, and suppressed Akt phosphorylation at Ser473, thereby inhibiting the Akt/GSK3β/β-catenin signaling pathway in bone mesenchymal stem cells...
March 10, 2017: Oncotarget
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