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Osteopontin

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https://www.readbyqxmd.com/read/28222767/knockdown-of-slc41a1-magnesium-transporter-promotes-mineralization-and-attenuates-magnesium-inhibition-during-osteogenesis-of-mesenchymal-stromal-cells
#1
Yu-Tzu Tsao, Ya-Yi Shih, Yu-An Liu, Yi-Shiuan Liu, Oscar K Lee
BACKGROUND: Magnesium is essential for numerous physiological functions. Magnesium exists mostly in bone and the amount is dynamically regulated by skeletal remodeling. Accelerating bone mass loss occurs when magnesium intake is insufficient; whereas high magnesium could lead to mineralization defects. However, the underlying magnesium regulatory mechanisms remain elusive. In the present study, we investigated the effects of high extracellular magnesium concentration on osteogenic differentiation of mesenchymal stromal/stem cells (MSCs) and the role of magnesium transporter SLC41A1 in the mineralization process...
February 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28222766/increased-cortical-lesion-load-and-intrathecal-inflammation-is-associated-with-oligoclonal-bands-in-multiple-sclerosis-patients-a-combined-csf-and-mri-study
#2
Gabriele Farina, Roberta Magliozzi, Marco Pitteri, Richard Reynolds, Stefania Rossi, Alberto Gajofatto, Maria Donata Benedetti, Francesco Facchiano, Salvatore Monaco, Massimiliano Calabrese
BACKGROUND: Although IgG oligoclonal bands (OCBs) in the cerebrospinal fluid (CSF) are a frequent phenomenon in multiple sclerosis (MS) patients, their relationship with grey matter lesions, intrathecal/meningeal inflammation and clinical evolution has not been clarified yet. The aim of our study was to assess the relationship between the OCBs, the inflammatory/neurodegenerative CSF profile at diagnosis, the cortical lesion load and the clinical evolution after 10 years. METHODS: This is a 10-year observational, cross-sectional study based on a combined MRI, cognitive and CSF profiling of the examined patients...
February 21, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28221099/sphingomyelin-phosphodiesterase-3-enhances-cytodifferentiation-of-periodontal-ligament-cells
#3
S Miyauchi, J Kitagaki, R Masumoto, A Imai, K Kobayashi, A Nakaya, S Kawai, C Fujihara, Y Asano, M Yamashita, M Yanagita, S Yamada, M Kitamura, S Murakami
Sphingomyelin phosphodiesterase 3 ( Smpd3), which encodes neutral sphingomyelinase 2 (nSMase2), is a key molecule for skeletal development as well as for the cytodifferentiation of odontoblasts and alveolar bone. However, the effects of nSMase2 on the cytodifferentiation of periodontal ligament (PDL) cells are still unclear. In this study, the authors analyzed the effects of Smpd3 on the cytodifferentiation of human PDL (HPDL) cells. The authors found that Smpd3 increases the mRNA expression of calcification-related genes, such as alkaline phosphatase (ALPase), type I collagen, osteopontin, Osterix (Osx), and runt-related transcription factor (Runx)-2 in HPDL cells...
March 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28214894/mechanical-loading-improves-tendon-bone-healing-in-a-rabbit-anterior-cruciate-ligament-reconstruction-model-by-promoting-proliferation-and-matrix-formation-of-mesenchymal-stem-cells-and-tendon-cells
#4
Fanglong Song, Dawei Jiang, Tianchen Wang, Yi Wang, Fengmei Chen, Guoxing Xu, Yifan Kang, Yinquan Zhang
BACKGROUND/AIMS: This study investigated the effect of mechanical stress on tendon-bone healing in a rabbit anterior cruciate ligament (ACL) reconstruction model as well as cell proliferation and matrix formation in co-culture of bone-marrow mesenchymal stem cells (BMSCs) and tendon cells (TCs). METHODS: The effect of continuous passive motion (CPM) therapy on tendon-bone healing in a rabbit ACL reconstruction model was evaluated by histological analysis, biomechanical testing and gene expressions at the tendon-bone interface...
February 16, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28214569/effect-of-testosterone-on-the-phenotypic-modulation-of-corpus-cavernosum-smooth-muscle-cells-in-a-castrated-rat-model
#5
Luhao Liu, Ermao Li, Futian Li, Lianmin Luo, Shankun Zhao, Ran Kang, Jintai Luo, Zhigang Zhao
OBJECTIVE: To investigate the effect of testosterone (T) on the phenotypic modulation of corpus cavernosum smooth muscle (CCSM) cells in a castrated rat model. METHODS: Thirty male Sprague-Dawley rats were randomly divided into three groups: control group, castration group, and castration with testosterone supplementation (castration+T) group. Erectile function, histological change and biochemical markers were assessed for phenotypic modulation of CCSM cells in corporal tissue...
February 15, 2017: Urology
https://www.readbyqxmd.com/read/28211493/25-hydroxyvitamin-d3-induces-osteogenic-differentiation-of-human-mesenchymal-stem-cells
#6
Yan-Ru Lou, Tai Chong Toh, Yee Han Tee, Hanry Yu
25-Hydroxyvitamin D3 [25(OH)D3] has recently been found to be an active hormone. Its biological actions are demonstrated in various cell types. 25(OH)D3 deficiency results in failure in bone formation and skeletal deformation. Here, we investigated the effect of 25(OH)D3 on osteogenic differentiation of human mesenchymal stem cells (hMSCs). We also studied the effect of 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3], a metabolite of 25(OH)D3. One of the vitamin D responsive genes, 25(OH)D3-24-hydroxylase (cytochrome P450 family 24 subfamily A member 1) mRNA expression is up-regulated by 25(OH)D3 at 250-500 nM and by 1α,25-(OH)2D3 at 1-10 nM...
February 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28210757/sildenafil-a-phosphodiesterase-type-5-inhibitor-downregulates-osteopontin-in-human-peripheral-blood-mononuclear-cells
#7
Beata Kaleta, Agnieszka Boguska
The aim of this study was to investigate the ability of sildenafil to regulate osteopontin (OPN) gene and protein in peripheral blood mononuclear cells (PBMCs) from healthy blood donors. OPN is expressed by a wide variety of cell types, including immune cells. OPN functions are linked to various physiological and pathological conditions. Sildenafil is a selective inhibitor of type 5 phosphodiesterase. Sildenafil has recently been found to have immunomodulatory effects in animal models and in studies performed in humans...
February 16, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28207598/newly-formed-bone-induced-by-recombinant-human-bone-morphogenetic-protein-2-a-histological-observation
#8
Azumi Hirata, Takaaki Ueno, Peter K Moy
PURPOSE: To observe, histologically, bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) in onlay grafted and sinus lifted alveolaris. MATERIAL AND METHODS: Eighteen patients were treated with rhBMP-2 at concentration 1.5 mg/mL with an absorbable collagen sponge (ACS). The treated bone was harvested with small trephine bur at 5 or 7 months after surgery for the micro Computer Scanning (CT) and light microscopic observation. RESULTS: Micro CT showed clearly 3-dimensional trabecular bone structure...
February 14, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28202997/-a-novel-tissue-engineered-bone-constructed-by-using-human-adipose-derived-stem-cells-and-biomimetic-calcium-phosphate-scaffold-coprecipitated-with-bone-morphogenetic-protein-2
#9
W R Jiang, X Zhang, Y S Liu, G Wu, Y J Ge, Y S Zhou
OBJECTIVE: To construct a novel biomimetic calcium phosphate (BioCaP) scaffold loaded with bone morphogenetic protein-2 (BMP-2), and to investigate its role in the osteogenesis of human adipose-derived stem cells (hASCs) in vitro and in vivo. METHODS: The BioCaP scaffold coprecipitated with BMP-2 (BMP-2-BioCaP) was constructed in this study. Field emission scanning electron microscopy (SEM) was used to analyze the morphology of the surfaces. The release kinetics was measured to evaluate the slow-release characteristics in vitro...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28202526/radiation-resistance-in-kras-mutated-lung-cancer-is-enabled-by-stem-like-properties-mediated-by-an-osteopontin-egfr-pathway
#10
Meng Wang, Jing Han, Lynnette Marcar, Josh Black, Qi Liu, Xiangyong Li, Kshithija Nagulapalli, Lecia V Sequist, Raymond H Mak, Cyril H Benes, Theodore S Hong, Kristin Gurtner, Mechthild Krause, Michael Baumann, Jing X Kang, Johnathan Whetstine, Henning Willers
Lung cancers with activating KRAS mutations are characterized by treatment resistance and poor prognosis. In particular, the basis for their resistance to radiation therapy is poorly understood. Here we describe a radiation resistance phenotype conferred by a stem-like subpopulation characterized by mitosis-like condensed chromatin (MLCC), high CD133 expression, invasive potential, and tumor-initiating properties. Mechanistic investigations defined a pathway involving osteopontin and the EGFR in promoting this phenotype...
February 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28198975/short-term-sodium-alendronate-administration-improves-the-peri-implant-bone-quality-in-osteoporotic-animals
#11
Danila de Oliveira, Jaqueline Suemi Hassumi, Pedro Henrique da Silva Gomes-Ferreira, Tárik Ocon Braga Polo, Gabriel Ramalho Ferreira, Leonardo Perez Faverani, Roberta Okamoto
Objective: The aim of this study was to evaluate the bone repair process at the bone/implant interface of osteoporotic rats treated with sodium alendronate through the analysis of microtomography, real time polymerase chain reactions and immunohistochemistry (RUNX2 protein, bone sialoprotein (BSP), alkaline phosphatase, osteopontin and osteocalcin). Material and Methods: A total of 42 rats were used and divided in to the following experimental groups: CTL: control group (rats submitted to fictitious surgery and fed with a balanced diet), OST: osteoporosis group (rats submitted to a bilateral ovariectomy and fed with a low calcium diet) and ALE: alendronate group (rats submitted to a bilateral ovariectomy, fed with a low calcium diet and treated with sodium alendronate)...
January 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28198555/involvement-of-cd147-in-alveolar-bone-remodeling-and-soft-tissue-degradation-in-experimental-periodontitis
#12
D Yang, R Liu, L Liu, H Liao, C Wang, Z Cao
BACKGROUND AND OBJECTIVE: The objective of this study was to investigate the possible roles of clusters of differentiation 147 (CD147) in bone resorption and mineralization through the bone markers of bone sialoprotein, osteocalcin, osteopontin and alkaline phosphatase (ALP), trabecular structure of alveolar bone and number of osteoclasts. We also investigated the effects of CD147 on inflammation and collagen breakdown. MATERIAL AND METHODS: Twenty-eight male Wistar rats were randomly divided into four groups of seven animals each: healthy group, periodontitis group, periodontitis + saline group and periodontitis + anti-CD147 groups...
February 15, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/28197374/tumor-derived-osteopontin-isoforms-cooperate-with-trp53-and-ccl2-to-promote-lung-metastasis
#13
Ioanna Giopanou, Ioannis Lilis, Vassilios Papaleonidopoulos, Theodora Agalioti, Nikolaos I Kanellakis, Nikolitsa Spiropoulou, Magda Spella, Georgios T Stathopoulos
The lungs are ubiquitous receptacles of metastases originating from various bodily tumors. Although osteopontin (SPP1) has been associated with tumor dissemination, the role of its isoforms in lung-directed metastasis is incompletely understood. We employed syngeneic mouse models of spontaneous and induced lung-targeted metastasis in C57BL/6 mice competent and deficient in both Spp1 alleles. Tumor-derived osteopontin expression was modulated using either stable anti-Spp1 RNA interference, or forced overexpression of intracellular and secreted Spp1 isoforms...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28194470/effect-of-vanadium-on-calcium-homeostasis-osteopontin-mrna-expression-and-bone-microarchitecture-in-diabetic-rats
#14
Cristina Sanchez-Gonzalez, Laura Moreno, Carlos Lopez-Chaves, Elena Nebot, Peter Pietschmann, Alba Rodriguez-Nogales, Julio Galvez, María Montes-Bayon, Alfredo Sanz-Medel, Juan Llopis
The aim of this study was to examine whether alterations caused by diabetes in calcium homeostasis, expression of osteopontin and the microarchitecture of bone are corrected by exposure to vanadium. Four study groups were examined over a period of five weeks: control (C), diabetic (DM), diabetic treated with 1 mg V per d (DMV), and diabetic treated with 3 mg V per d (DMVH). Vanadium was supplied in drinking water as bis(maltolato)oxovanadium(iv). Calcium was measured in the food, faeces, urine, serum, kidneys, liver, muscles, and femur...
February 14, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28194185/osteopontin-relation-between-adipose-tissue-and-bone-homeostasis
#15
REVIEW
Carolina De Fusco, Antonietta Messina, Vincenzo Monda, Emanuela Viggiano, Fiorenzo Moscatelli, Anna Valenzano, Teresa Esposito, Chieffi Sergio, Giuseppe Cibelli, Marcellino Monda, Giovanni Messina
Osteopontin (OPN) is a multifunctional protein mainly associated with bone metabolism and remodeling. Besides its physiological functions, OPN is implicated in the pathogenesis of a variety of disease states, such as obesity and osteoporosis. Importantly, during the last decades obesity and osteoporosis have become among the main threats to health worldwide. Because OPN is a protein principally expressed in cells with multifaceted effects on bone morphogenesis and remodeling and because it seems to be one of the most overexpressed genes in the adipose tissue of the obese contributing to osteoporosis, this mini review will highlight recent insights about relation between adipose tissue and bone homeostasis...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28193231/association-of-osteopontin-with-specific-prognostic-factors-and-survival-in-adjuvant-breast-cancer-trials-of-the-hellenic-cooperative-oncology-group
#16
Amanda Psyrri, Konstantine T Kalogeras, Ralph M Wirtz, George Kouvatseas, Georgia Karayannopoulou, Anna Goussia, Flora Zagouri, Elke Veltrup, Eleni Timotheadou, Helen Gogas, Angelos Koutras, Georgios Lazaridis, Christos Christodoulou, George Pentheroudakis, Panagiota Economopoulou, Apostolos Laskarakis, Petroula Arapantoni-Dadioti, Anna Batistatou, Maria Sotiropoulou, Gerasimos Aravantinos, Pavlos Papakostas, Paris Kosmidis, Dimitrios Pectasides, George Fountzilas
BACKGROUND: The shift towards an earlier diagnosis of breast cancer (BC) highlights the need for biomarkers that would identify patients at risk for relapse and metastatic spread and indicate the potential value of additional treatment strategies. Osteopontin (OPN) is a matricellular protein that has been suggested to be a potential biomarker in BC. In the present study, we used archived BC patient samples to assess the clinical utility of OPN. METHODS: Formalin-fixed paraffin-embedded tumor tissue samples from 975 patients were collected from two large phase III randomized adjuvant chemotherapy trials (HE10/97 and HE10/00) that included patients with high risk BC...
February 13, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28192876/determination-of-16-serum-angiogenic-factors-in-stage-i-non-small-cell-lung-cancer-using-a-bead-based-multiplex-immunoassay
#17
Agnieszka Klupczynska, Paweł Dereziński, Jan Matysiak, Wojciech Dyszkiewicz, Mariusz Kasprzyk, Zenon J Kokot
The aim of the study was to identify significant abnormalities in angiogenic factor profiles occurring at early-stage non-small cell lung cancer (NSCLC). Contrary to the previous studies, our research included patients with only stage I NSCLC, which allowed for estimating the utility of circulating angiogenic factors in early NSCLC detection. The investigation was performed in serum samples collected from individuals with untreated NSCLC (n=41) and a matched control group of healthy individuals (n=61). All patients had histopathologically-confirmed stage IA or IB NSCLC...
February 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28192120/osteopontin-attenuates-acute-gastrointestinal-graft-versus-host-disease-by-preventing-apoptosis-of-intestinal-epithelial-cells
#18
Kentaro Kawakami, Naoki Minami, Minoru Matsuura, Tomoya Iida, Takahiko Toyonaga, Kanna Nagaishi, Yoshiaki Arimura, Mineko Fujimiya, Toshimitsu Uede, Hiroshi Nakase
BACKGROUND AND AIMS: Acute graft-versus-host disease (GVHD) is a major complication after allogeneic hematopoietic stem cell transplantation, which often targets gastrointestinal (GI) tract. Osteopontin (OPN) plays an important physiological role in the efficient development of Th1 immune responses and cell survival by inhibiting apoptosis. The role of OPN in acute GI-GVHD is poorly understood. In the present study, we investigated the role of OPN in donor T cells in the pathogenicity of acute GI-GVHD...
February 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28191762/periodontal-tissue-regeneration-using-syngeneic-adipose-derived-stromal-cells-in-a-mouse-model
#19
Mathieu Lemaitre, Paul Monsarrat, Vincent Blasco-Baque, Pascale Loubières, Rémy Burcelin, Louis Casteilla, Valérie Planat-Bénard, Philippe Kémoun
Current treatment of periodontitis is still associated with a high degree of variability in clinical outcomes. Recent advances in regenerative medicine by mesenchymal cells, including adipose stromal cells (ASC) have paved the way to improved periodontal regeneration (PD) but little is known about the biological processes involved. Here, we aimed to use syngeneic ASCs for periodontal regeneration in a new, relevant, bacteria-induced periodontitis model in mice. Periodontal defects were induced in female C57BL6/J mice by oral gavage with periodontal pathogens...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191670/involvement-of-the-phosphoinositide-3-kinase-akt-signaling-pathway-in-bone-morphogenetic-protein-9-stimulated-osteogenic-differentiation-and-stromal-cell-derived-factor-1-production-in-human-periodontal-ligament-fibroblasts
#20
Kirara Furue, Kotaro Sena, Kenji Sakoda, Toshiaki Nakamura, Kazuyuki Noguchi
Recent studies have shown that bone morphogenetic protein 9 (BMP-9) can induce osteogenic differentiation in human periodontal stem cells and human periodontal ligament fibroblasts (PDLFs). Bone morphogenetic protein 9 may be used in periodontal tissue regeneration because of its potent osteoinductive ability. Human periodontal ligament cells also have been demonstrated to produce stromal cell-derived factor 1 (SDF-1), which is important for stem-cell homing and recruitment to injured sites. In the present study, we examined the involvement of the phosphoinositide 3-kinase (PI3K)/Akt signaling axis in osteogenic differentiation and SDF-1 production in human PDLFs stimulated with BMP-9 in osteogenic medium supplemented with dexamethasone and ascorbic acid...
February 13, 2017: European Journal of Oral Sciences
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