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Osteopontin

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https://www.readbyqxmd.com/read/28652837/a-pilot-study-of-zoledronic-acid-in-the-treatment-of-patients-with-advanced-malignant-pleural-mesothelioma
#1
Muhammad Omer Jamil, Mary S Jerome, Deborah Miley, Katri S Selander, Francisco Robert
PURPOSE: Malignant pleural mesothelioma (MPM) is a rare malignancy with a dismal median survival of <12 months with current therapy. Single and combination chemotherapy regimens have shown only modest clinical benefit. In preclinical studies, nitrogen-containing bisphosphonates (zoledronic acid) inhibit growth of mesothelioma cells by different mechanisms: inhibition of mevalonate pathway, inhibition of angiogenesis, activation of apoptosis through caspase activation, and alteration in activity of matrix metalloproteinases, thereby affecting invasiveness of cancer cells...
2017: Lung Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/28649629/rhogtpase-stimulation-is-associated-with-strontium-chloride-treatment-to-counter-simulated-microgravity-induced-changes-in-multipotent-cell-commitment
#2
Fiona Louis, Wafa Bouleftour, Aline Rattner, Marie-Thérèse Linossier, Laurence Vico, Alain Guignandon
Microgravity-related cytoskeletal disorganization is associated with an altered balance between osteoblastogenesis and adipogenesis of multipotent cells. Strontium chloride is known to increase osteoblastogenesis and repress adipogenesis, but its effects in microgravity-related conditions have not been established. Our goal was to investigate early events in this process, focusing on RhoGTPases as controllers of cytoskeletal organization leading to stem cell commitment. We cultivated C3H10T1/2 on microspheres using a rotating wall vessel bioreactor (NASA) in order to simulate microgravity-related conditions in adipogenesis and osteoblastogenesis conditions independently...
2017: NPJ Microgravity
https://www.readbyqxmd.com/read/28645880/biomarkers-of-airway-and-systemic-inflammation-in-obese-asthmatic-paediatric-patients
#3
H T Nacaroglu, O B Gayret, M Erol, O Buke, O Zengi, M Tasdemir, Z Tasdemir, O Yigit
BACKGROUND: It is thought that airway inflammation is more common in obese asthmatic patients because inflammation is harder to control and does not respond well to glucocorticoid treatment. OBJECTIVE: This study's aim was to investigate the effect of obesity on airway and systemic inflammation in children with asthma and to identify the biomarkers that play a role in this inflammation. METHODS: The study included patients aged 6-16 years who were diagnosed with asthma in the paediatric allergy outpatient clinic of Bagcilar Training and Research Hospital in Turkey...
June 20, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28644763/methyltransferase-g9a-regulates-osteogenesis-via-twist-gene-repression
#4
N Higashihori, B Lehnertz, A Sampaio, T M Underhill, F Rossi, J M Richman
Here we investigate the role of epigenetic factors in controlling the timing of cranial neural crest cell differentiation. The gene coding for histone H3 lysine 9 methyltransferase G9A was conditionally deleted in neural crest cells with Wnt1-Cre. The majority of homozygous-null animals survived to birth but thereafter failed to thrive. Phenotypic analysis of postnatal animals revealed that the mutants displayed incomplete ossification and 20% shorter jaws as compared to their wild-type littermates. At E13...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28643442/the-effect-of-collagen-coating-on-titanium-with-nanotopography-on-in-vitro-osteogenesis
#5
Daniel G Costa, Emanuela P Ferraz, Rodrigo P F Abuna, Paulo T de Oliveira, Marco Morra, Marcio M Beloti, Adalberto L Rosa
Several studies have shown the positive effects of Ti either with nanotopography or coated with collagen on osteoblast differentiation. Thus, we hypothesized that the association of nanotopography with collagen may increase the in vitro osteogenesis on Ti surface. Ti discs with nanotopography with or without collagen coating were characterized by scanning electron microscopy and atomic force microscopy. Rat calvaria-derived osteoblastic cells were cultured on both Ti surfaces for up to 14 days and the following parameters were evaluated: cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization, protein expression of bone sialoprotein (BSP) and osteopontin (OPN), and gene expression of collagen type 1a (Coll1a), runt related transcription factor 2 (Runx2), osterix (OSX), osteocalcin (OC), Ki67, Survivin and Bcl2 associated X protein (BAX)...
June 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28641514/clinically-relevant-biomarkers-in-acute-heart-failure-an-update
#6
Agnibh Mukherji, Uzair Ansari, Martin Borggrefe, Ibrahim Akin, Michael Behnes
Acute heart failure (AHF) has been one of the fields, where owing to difficulties in diagnosis, prognostic stratification and management the associated morbidity and mortality remains unusually high. The advent of novel biomarkers apart from the traditional B-type natriuretic peptide and N-terminal proBNP involved in myocardial injury, neuro-hormonal activation, and ventricular remodeling augurs immense hope in redefining our approach towards AHF. METHODS: Biomarkers like midregional proatrial natriuretic peptide, soluble ST2, highly-sensitive troponin, and midregional proadrenomedullin are some such examples that have passed various stages of substantiation while there is an array of potential biomarkers in nascent stages like osteopontin, CTGF, GDF-15 and TGF beta-1 awaiting further reiteration...
June 22, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28639437/ultrasound-assisted-green-economic-synthesis-of-hydroxyapatite-nanoparticles-using-eggshell-bio-waste-and-study-of-mechanical-and-biological-properties-for-orthopaedic-applications
#7
Vijay H Ingole, Kamal Hany Hussein, Anil A Kashale, Kalyani Ghule, Tomaž Vuherer, Vanja Kokol, Jia-Yaw Chang, Yong-Chien Ling, Aruna Vinchurkar, Hom N Dhakal, Anil V Ghule
Nanostructured hydroxyapatite (HAp) is most favorable candidate biomaterial for bone tissue engineering because of its bioactive and osteoconductive properties. Herein, we report for the first time ultrasound assisted facile and economic approach for the synthesis of nanocrystalline hydroxyapatite (Ca10 (PO4 )6 (OH)2 ) using recycled eggshell bio-waste referred as EHAp. The process involves the reaction of eggshell bio-waste as a source of calcium and ammonium dihydrogen orthophosphate as a phosphate source...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28638999/osteopontin-and-clusterin-levels-in-type-2-diabetes-mellitus-differential-association-with-peripheral-autonomic-nerve-function
#8
Raelene E Maser, M James Lenhard, Ryan T Pohlig, P Babu Balagopal
Osteopontin (OPN) and clusterin are secreted glycoproteins potentially associated with nerve function. Sudomotor dysfunction is associated with the development of foot ulcerations. The purpose of this study was to investigate the potential relationship of OPN and clusterin with sudomotor function (i.e., autonomic nerves that control sweating) in participants with type 2 diabetes mellitus (T2DM). Sudomotor function was assessed using SUDOSCAN® which measures electrochemical skin conductance (ESC) of the hands and feet...
June 21, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/28638450/osteopontin-is-critical-for-hyperactive-mtor-induced-tumorigenesis-in-oral-squamous-cell-carcinoma
#9
Ning Gan, Sihai Zou, Wenming Hang, Deqin Yang, Xuemei Zhang, Yibing Yin
Mechanistic target of rapamycin (mTOR) plays a critical role in the development of oral squamous cell carcinoma (OSCC), but the underlying mechanisms remain poorly understood. Here we have demonstrated that the expression of osteopontin (OPN) was dramatically up-regulated in OSCC tissues and cell lines. Moreover, reduction of OPN suppressed cell proliferation, colony formation, and in vivo tumorigenic ability of OSCC cell lines Tca8113. In addition, there was a strong positive correlation between mTORC1 activity and OPN expression in OSCC tissues and cell lines...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28634845/histomorphometric-and-immunohistochemical-evaluation-of-collagen-containing-xenogeneic-bone-blocks-used-for-lateral-bone-augmentation-in-staged-implant-placement
#10
Alberto Ortiz-Vigón, Sergio Martinez-Villa, Iñaki Suarez, Fabio Vignoletti, Mariano Sanz
BACKGROUND: The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures. METHODS: In 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed using CCXBB as bone replacement grafts. Twenty-six weeks postoperatively, a re-entry procedure was performed to evaluate the bone width for adequate implant placement and two histological specimens were retrieved from each patient, one being processed for ground sectioning and the other for decalcified paraffin-included sections...
December 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/28631614/3d-printed-bioceramic-scaffolds-with-antibacterial-and-osteogenic-activity
#11
Yongliang Zhang, Dong Zhai, Mengchi Xu, Qingqiang Yao, Huiying Zhu, Jiang Chang, Chengtie Wu
Bacterial infection poses a significant risk with the wide application of bone graft materials. Designing bone grafts with good antibacterial performance and excellent bone-forming activity is of particular significance for bone tissue engineering. In our study, a 3D printing method was used to prepare β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Silver (Ag) nanoparticles were uniformly dispersed on graphene oxide (GO) to form a homogeneous nanocomposite (named Ag@GO) with different Ag-to-graphene oxide mass ratios, with this being synthesized via the liquid chemical reduction approach...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28627641/osteopontin-deficiency-protects-mice-from-cholesterol-gallstone-formation-by-reducing-expression-of-intestinal-npc1l1
#12
Jing Lin, Wei-Qing Shao, Qing-Zhi Chen, Wen-Wei Zhu, Lu Lu, Hu-Liang Jia, Jin-Hong Chen
Homeostasis of cholesterol is regulated by absorption in the intestine and synthesis in the liver. The authors previously demonstrated that OPN (osteopontin) exhibits the ability to alter hepatic cholesterol metabolism, thus affecting cholesterol gallstone formation in mice. The present study investigated the role of OPN in cholesterol gallstone formation, focusing on its effect on intestinal absorption of cholesterol. OPN gene knockout (OPN‑/‑) mice and wild‑type mice were respectively fed with a chow or lithogenic diet (LD) for 8 weeks...
June 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28625769/mechanisms-of-aortic-dissection-smooth-muscle-cell-phenotype-switch
#13
Zhao An, Yang Liu, Zhi-Gang Song, Hao Tang, Yang Yuan, Zhi-Yun Xu
OBJECTIVE: To investigate the expression of Nanog homeobox (NANOG) in thoracic aortic dissection (TAD) and the role of NANOG in regulating human aortic vascular smooth muscle cells (VSMCs) phenotype switch. METHODS: Aortic specimens were collected from 20 patients undergoing TAD and 10 controls. VSMCs were isolated by adherent cultivation approach. The expression of NANOG, osteopontin (OPN), and VSMCs phenotype markers were determined by quantitative real-time polymerase chain reaction, Western blot, immunohistochemistry, and immunofluorescence...
May 25, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28622916/osteogenic-potential-of-platelet-rich-plasma-in-dental-stem-cell-cultures
#14
L Otero, N Carrillo, J L Calvo-Guirado, J Villamil, R A Delgado-Ruíz
The purpose of this study was to analyse the potential of platelet-rich plasma (PRP) culture media to induce osteogenic differentiation of periodontal ligament stem cells and dental pulp stem cells compared with four other methods of culture. Both types of cell were collected from 35 healthy patients and cultured in five different media (Dulbecco's modified eagle's medium (DMEM); DMEM and melatonin; DMEM and PRP; DMEM and ascorbic acid 200μmol; DMEM and l-ascorbate 2-phosphate 50μmol). Cells were characterised by flow cytometry...
June 13, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28620344/the-role-of-osteopontin-and-its-gene-on-glucocorticoid-response-in-myasthenia-gravis
#15
Yanchen Xie, Hai-Feng Li, Liang Sun, Linda L Kusner, Shuhui Wang, Yunxiao Meng, Xu Zhang, Yu Hong, Xiang Gao, Yao Li, Henry J Kaminski
Biomarkers that assess treatment response for patients with the autoimmune disorder, myasthenia gravis (MG), have not been evaluated to a significant extent. We hypothesized the pro-inflammatory cytokine, osteopontin (OPN), may be associated with variability of response to glucocorticoids (GCs) in patients with MG. A cohort of 250 MG patients treated with standardized protocol of GCs was recruited, and plasma OPN and polymorphisms of its gene, secreted phosphoprotein 1 (SPP1), were evaluated. Mean OPN levels were higher in patients compared to healthy controls...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28620244/specific-ablation-of-mouse-fam20c-in-cells-expressing-type-i-collagen-leads-to-skeletal-defects-and-hypophosphatemia
#16
Peihong Liu, Su Ma, Hua Zhang, Chao Liu, Yongbo Lu, Li Chen, Chunlin Qin
FAM20C mutations in humans cause Raine syndrome and our previous studies showed that global inactivation of mouse Fam20C led to bone and dental defects. By crossbreeding 2.3  kb Col 1a1-Cre mice with Fam20C (flox/flox) mice, we created 2.3  kb Col 1a1-Cre;Fam20C (foxl/flox) (cKO) mice, in which Fam20C was inactivated in cells expressing Type I collagen. This study showed that the long bones of cKO mice were shorter and had a lower level of mineralization compared to the normal mice. The collagen fibrils in Fam20C-deficient bone were disorganized and thicker while the growth plate cartilage in cKO mice was disorganized and wider compared to the normal mice...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28618926/diagnostic-relevance-of-a-novel-multiplex-immunoassay-panel-in-breast-cancer
#17
Natalie Hermann, Katja Dressen, Lars Schroeder, Manuel Debald, Frank A Schildberg, Gisela Walgenbach-Bruenagel, Karina Hettwer, Steffen Uhlig, Walther Kuhn, Gunther Hartmann, Stefan Holdenrieder
Multiple factors contribute to the development and progression of breast cancer. Markers of tumor growth and invasion, cell death, immune activation, and angiogenesis can be assessed in parallel by a novel multiplex immunoassay panel. The diagnostic performance of a multiplex cancer biomarker magnetic bead panel comprising 24 tumor associated parameters was evaluated in sera of 154 women including 77 patients with breast cancer, 10 with precancerous lesions, 31 with benign breast diseases, and 36 healthy controls...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28617070/oral-subchronic-exposure-to-silver-nanoparticles-causes-renal-damage-through-apoptotic-impairment-and-necrotic-cell-death
#18
Ratnakar Tiwari, Radha Dutt Singh, Hafizurrahman Khan, Siddhartha Gangopadhyay, Sandeep Mittal, Vikas Singh, Nidhi Arjaria, Jai Shankar, Somendu Kumar Roy, Dhirendra Singh, Vikas Srivastava
Silver nanoparticles (AgNPs) are one of the most widely used nanomaterials. Following oral exposure, AgNPs can accumulate in various organs including kidneys where they show gender specific accumulation. There is limited information on their effect on renal system following long-term animal exposure especially at the ultramicroscopic and molecular level. In this study, we have assessed the effect of 60 days oral AgNPs treatment on kidneys of female Wistar rats at doses of 50 ppm and 200 ppm that are below previously reported lowest observed adverse effect level (LOAEL)...
June 15, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28616648/protective-effect-of-dietary-polyphenol-caffeic-acid-on-ethylene-glycol-induced-kidney-stones-in-rats
#19
Fauzia Yasir, Atia-Tul- Wahab, M Iqbal Choudhary
Dietary polyphenol caffeic acid (1) has been reported for various pharmacological activities. The aim of the current study was to investigate the effect of caffeic acid (1) on ethylene glycol-induced renal stones in rats. For the study, male Wistar rats were divided into seven groups; normal, pathological, and standard drug controls, and preventive and curative groups. Normal control group received drinking water for 8 weeks. Pathological, standard drug, preventive, and curative groups received 0.75% ethylene glycol in drinking water for the induction of calcium oxalate stone formation, along with the regular diet...
June 14, 2017: Urolithiasis
https://www.readbyqxmd.com/read/28608628/the-role-of-microrna-143-5p-in-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts-by-targeting-runx2-via-the-opg-rankl-signaling-pathway
#20
Fu-Liang Zhan, Xin-Yang Liu, Xing-Bo Wang
This study aims to elucidate the mechanisms by which microRNA-143-5p (miR-143-5p) targets runt-related transcription factor 2 (Runx2) in the differentiation of dental pulp stem cells (DPSCs) into odontoblasts, through regulating the osteoprotegerin receptor activator of the nuclear factor-κB ligand (OPG/RANKL) signaling pathway. Following transfection, DPSCs were divided into blank, control, miR-143-5p mimics, miR-143-5p inhibitors, miR-143-5p inhibitors + siRunx2 and siRunx2 groups. Alkaline phosphatase (ALP) activity and mineralized nodules were detected using ALP kit and alizarin red staining...
June 13, 2017: Journal of Cellular Biochemistry
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