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https://www.readbyqxmd.com/read/27916571/impact-and-seasonality-of-human-rhinovirus-infection-in-hospitalized-patients-for-two-consecutive-years
#1
Jaqueline Leotte, Hygor Trombetta, Heloisa Z Faggion, Bernardo M Almeida, Meri B Nogueira, Luine R Vidal, Sonia M Raboni
OBJECTIVES: To report epidemiological features, clinical characteristics, and outcomes of human rhinovirus (HRV) infections in comparison with other community acquired respiratory virus (CRV) infections in patients hospitalized for two consecutive years. METHODS: This was a cross-sectional study. Clinical, epidemiological, and laboratory data of patients hospitalized with acute respiratory syndrome in a tertiary care hospital from 2012 to 2013 were reviewed. RESULTS: HRV was the most common CRV observed (36%, 162/444) and was present in the majority of viral co-detections (69%, 88/128), mainly in association with human enterovirus (45%)...
December 1, 2016: Jornal de Pediatria
https://www.readbyqxmd.com/read/27910696/a-new-setup-for-localized-implantation-and-live-characterization-of-kev-energy-multiply-charged-ions-at-the-nanoscale
#2
S Guillous, C Bourin, B Ban D'Etat, A Benyagoub, A Cassimi, C Feierstein, E Gardés, E Giglio, S Girard, C Grygiel, A Houel, H Lebius, A Méry, I Monnet, J-M Ramillon, J Rangama, F Ropars, E Verzeroli, M Viteau, A Delobbe
An innovative experimental setup, PELIICAEN, allowing the modification of materials and the study of the effects induced by multiply charged ion beams at the nanoscale is presented. This ultra-high vacuum (below 5 × 10(-10) mbar) apparatus is equipped with a focused ion beam column using multiply charged ions and a scanning electron microscope developed by Orsay Physics, as well as a scanning probe microscope. The dual beam approach coupled to the scanning probe microscope achieves nanometer scale in situ topological analysis of the surface modifications induced by the ion beams...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27904883/interactions-of-respiratory-viruses-and-the-nasal-microbiota-during-the-first-year-of-life-in-healthy-infants
#3
Insa Korten, Moana Mika, Shkipe Klenja, Elisabeth Kieninger, Ines Mack, Maria Teresa Barbani, Meri Gorgievski, Urs Frey, Markus Hilty, Philipp Latzin
Traditional culture techniques have shown that increased bacterial colonization is associated with viral colonization; however, the influence of viral colonization on the whole microbiota composition is less clear. We thus aimed to understand the interaction of viral infections and the nasal microbiota in early life to appraise their roles in disease development. Thirty-two healthy, unselected infants were included in this prospective longitudinal cohort study within the first year of life. Biweekly nasal swabs (n = 559) were taken, and the microbiota was analyzed by 16S rRNA pyrosequencing, and 10 different viruses and 2 atypical bacteria were characterized by real-time PCR (combination of seven duplex samples)...
November 2016: MSphere
https://www.readbyqxmd.com/read/27895398/dysregulation-of-innate-immunity-in-ulcerative-colitis-patients-who-fail-anti-tumor-necrosis-factor-therapy
#4
Angela C Baird, Dominic Mallon, Graham Radford-Smith, Julien Boyer, Thierry Piche, Susan L Prescott, Ian C Lawrance, Meri K Tulic
AIM: To study the innate immune function in ulcerative colitis (UC) patients who fail to respond to anti-tumor necrosis factor (TNF) therapy. METHODS: Effects of anti-TNF therapy, inflammation and medications on innate immune function were assessed by measuring peripheral blood mononuclear cell (PBMC) cytokine expression from 18 inflammatory bowel disease patients pre- and 3 mo post-anti-TNF therapy. Toll-like receptor (TLR) expression and cytokine production post TLR stimulation was assessed in UC "responders" (n = 12) and "non-responders" (n = 12) and compared to healthy controls (n = 12)...
November 7, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27880982/safety-and-efficacy-of-ruxolitinib-in-splanchnic-vein-thrombosis-associated-with-myeloproliferative-neoplasms
#5
Lisa Pieri, Chiara Paoli, Umberto Arena, Fabio Marra, Fabio Mori, Mery Zucchini, Stefano Colagrande, Alessandro Castellani, Arianna Masciulli, Vittorio Rosti, Valerio De Stefano, Silvia Betti, Guido Finazzi, Maria Luisa Ferrari, Elisa Rumi, Marco Ruggeri, Ilaria Nichele, Paola Guglielmelli, Rajmonda Fjerza, Carmela Mannarelli, Tiziana Fanelli, Lucia Merli, Giuditta Corbizi Fattori, Margherita Massa, Giuseppe Cimino, Alessandro Rambaldi, Giovanni Barosi, Mario Cazzola, Tiziano Barbui, Alessandro M Vannucchi
Splanchnic vein thrombosis (SVT) is one of the vascular complications of myeloproliferative neoplasms (MPN). We designed a phase 2 clinical trial to evaluate safety and efficacy of ruxolitinib in reducing splenomegaly and improving disease-related symptoms in patients with MPN-associated SVT. Patients, diagnosed with myelofibrosis in 12 cases, polycythemia vera in 5 and essential thrombocythemia in 4, received ruxolitinib for 24 weeks in the core study period. Spleen volume was assessed by magnetic resonance imaging (MRI) and splanchnic vein circulation by echo-Doppler analysis...
November 23, 2016: American Journal of Hematology
https://www.readbyqxmd.com/read/27873065/decline-in-territory-size-and-fecundity-as-a-response-to-carrying-capacity-in-an-endangered-songbird
#6
Stefanie A Hartmann, Steffen Oppel, Gernot Segelbacher, Mery E Juiña, H Martin Schaefer
Density-dependent processes are fundamental mechanisms for the regulation of populations. Ecological theories differ in their predictions on whether increasing population density leads to individual adjustments of survival and reproductive output or to dominance and monopolization of resources. Here, we use a natural experiment to examine which factors limit population growth in the only remaining population of the endangered pale-headed brush finch (Atlapetes pallidiceps). For three distinct phases (a phase of population suppression, 2001-2002; expansion due to conservation management, 2003-2008; and equilibrium phase, 2009-2014), we estimated demographic parameters with an integrated population model using population size, the proportion of successfully breeding pairs and their productivity, territory size, and mark-recapture data of adult birds...
November 21, 2016: Oecologia
https://www.readbyqxmd.com/read/27859692/louse-borne-relapsing-fever-in-finland-in-two-asylum-seekers-from-somalia
#7
Jukka Hytönen, Tamim Khawaja, Juha O Grönroos, Anna Jalava, Seppo Meri, Jarmo Oksi
We report two cases of louse-borne relapsing fever (LBRF) in young Somali asylum seekers having recently arrived to Finland. They had sought medical attention for a febrile illness. Blood smears were examined for suspected malaria, but instead, spirochete shaped bacteria were observed. The bacteria were confirmed as Borrelia recurrentis by PCR and sequencing. The patients survived, but their treatment was complicated by Jarisch-Herxheimer reaction. We conclude that LBRF must be considered as a diagnostic option in febrile refugees also in the northernmost parts of Europe...
November 16, 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27856057/mapping-the-birch-and-grass-pollen-seasons-in-the-uk-using-satellite-sensor-time-series
#8
Nabaz R Khwarahm, Jadunandan Dash, C A Skjøth, R M Newnham, B Adams-Groom, K Head, Eric Caulton, Peter M Atkinson
Grass and birch pollen are two major causes of seasonal allergic rhinitis (hay fever) in the UK and parts of Europe affecting around 15-20% of the population. Current prediction of these allergens in the UK is based on (i) measurements of pollen concentrations at a limited number of monitoring stations across the country and (ii) general information about the phenological status of the vegetation. Thus, the current prediction methodology provides information at a coarse spatial resolution only. Most station-based approaches take into account only local observations of flowering, while only a small number of approaches take into account remote observations of land surface phenology...
November 14, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27851258/1623-common-hurdles-in-the-postoperative-management-of-a-patient-with-a-total-artificial-heart
#9
Marissa Mery, Siavosh Saatee, Sonia Nhieu
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27830327/lack-of-mutation-of-dicer1-and-foxl2-genes-in-microcystic-stromal-tumor-of-the-ovary
#10
Alexandra Meurgey, Françoise Descotes, Eliane Mery-Lamarche, Mojgan Devouassoux-Shisheboran
Microcystic stromal tumors (MCST), first described in 2009 by Irving et al., are rare ovarian neoplasms. The entity was introduced into the 2014 WHO classification of tumors of female reproductive organs in the group of sex cord-stromal tumors, which is rather heterogeneous. We studied three cases of ovarian tumor with the characteristic morphological features and immunohistochemical marker profiles of MCST. The three tumors showed micro, and macrocystic patterns with solid areas, and were composed of small round to spindle-shaped cells, without atypia...
November 9, 2016: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/27828524/estimation-of-daily-photosynthetically-active-radiation-par-in-presence-of-low-to-high-aerosol-loads-application-to-olci-like-satellite-data
#11
Tristan Harmel, Malik Chami
Estimation of daily photosynthetically active radiation (PAR) is of primary importance for monitoring the ocean primary production and the subsequent production of carbon by phytoplankton at global scale from remote sensing ocean color sensors. On the other hand, aerosol abundance and composition play a critical role in the modulation of PAR. In this study, an original algorithm, so-called OLCIPAR, is proposed for routinely determining the daily PAR from optical satellite sensors such as the OLCI sensor aboard Sentinel-3 (ESA)...
October 31, 2016: Optics Express
https://www.readbyqxmd.com/read/27819657/culture-of-previously-uncultured-members-of-the-human-gut-microbiota-by-culturomics
#12
Jean-Christophe Lagier, Saber Khelaifia, Maryam Tidjani Alou, Sokhna Ndongo, Niokhor Dione, Perrine Hugon, Aurelia Caputo, Frédéric Cadoret, Sory Ibrahima Traore, El Hadji Seck, Gregory Dubourg, Guillaume Durand, Gaël Mourembou, Elodie Guilhot, Amadou Togo, Sara Bellali, Dipankar Bachar, Nadim Cassir, Fadi Bittar, Jérémy Delerce, Morgane Mailhe, Davide Ricaboni, Melhem Bilen, Nicole Prisca Makaya Dangui Nieko, Ndeye Mery Dia Badiane, Camille Valles, Donia Mouelhi, Khoudia Diop, Matthieu Million, Didier Musso, Jônatas Abrahão, Esam Ibraheem Azhar, Fehmida Bibi, Muhammad Yasir, Aldiouma Diallo, Cheikh Sokhna, Felix Djossou, Véronique Vitton, Catherine Robert, Jean Marc Rolain, Bernard La Scola, Pierre-Edouard Fournier, Anthony Levasseur, Didier Raoult
Metagenomics revolutionized the understanding of the relations among the human microbiome, health and diseases, but generated a countless number of sequences that have not been assigned to a known microorganism(1). The pure culture of prokaryotes, neglected in recent decades, remains essential to elucidating the role of these organisms(2). We recently introduced microbial culturomics, a culturing approach that uses multiple culture conditions and matrix-assisted laser desorption/ionization-time of flight and 16S rRNA for identification(2)...
November 7, 2016: Nature Microbiology
https://www.readbyqxmd.com/read/27806057/occurrence-of-anti-drug-antibodies-against-interferon-beta-and-natalizumab-in-multiple-sclerosis-a-collaborative-cohort-analysis
#13
Delphine Bachelet, Signe Hässler, Cyprien Mbogning, Jenny Link, Malin Ryner, Ryan Ramanujam, Michael Auer, Poul Erik Hyldgaard Jensen, Nils Koch-Henriksen, Clemens Warnke, Kathleen Ingenhoven, Dorothea Buck, Verena Grummel, Andy Lawton, Naoimh Donnellan, Agnès Hincelin-Mery, Dan Sikkema, Marc Pallardy, Bernd Kieseier, Bernard Hemmer, Hans Peter Hartung, Per Soelberg Sorensen, Florian Deisenhammer, Pierre Dönnes, Julie Davidson, Anna Fogdell-Hahn, Philippe Broët
Immunogenicity of biopharmaceutical products in multiple sclerosis is a frequent side effect which has a multifactorial etiology. Here we study associations between anti-drug antibody (ADA) occurrence and demographic and clinical factors. Retrospective data from routine ADA test laboratories in Sweden, Denmark, Austria and Germany (Dusseldorf group) and from one research study in Germany (Munich group) were gathered to build a collaborative multi-cohort dataset within the framework of the ABIRISK project. A subset of 5638 interferon-beta (IFNβ)-treated and 3440 natalizumab-treated patients having data on at least the first two years of treatment were eligible for interval-censored time-to-event analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27796724/effects-of-methanolic-extract-from-leaves-of-rubus-imperialis-in-dss-induced-colitis-in-mice
#14
Luisa Mota da Silva, Lincon Bordignon Somensi, Thaise Boeing, Cristiane Barp, Valdir Cechinel-Filho, Rivaldo Niero, Sérgio Faloni de Andrade
This study investigated the effects of Rubus imperialis, a berry known as "amora-branca", in colitis dextran sulfate sodium (DSS)-induced in mice. Animals were treated orally with vehicle (water), 5-aminosalicylic acid (100 mg/kg) or methanolic extract from leaves of R. imperialis (MERI, 100 mg/kg), once a day during seven days. The disease activity index (DAI) was observed daily. Colons were collected for histological, histochemical and biochemical analysis. The administration of MERI exacerbated colitis, as indicated by DAI heightened weight loss and increased histological colonic injury...
October 31, 2016: Inflammopharmacology
https://www.readbyqxmd.com/read/27793574/resource-requirements-for-cancer-registration-in-areas-with-limited-resources-analysis-of-cost-data-from-four-low-and-middle-income-countries
#15
Florence K L Tangka, Sujha Subramanian, Patrick Edwards, Maggie Cole-Beebe, D Maxwell Parkin, Freddie Bray, Rachael Joseph, Les Mery, Mona Saraiya
BACKGROUND: The key aims of this study were to identify sources of support for cancer registry activities, to quantify resource use and estimate costs to operate registries in low- and middle-income countries (LMIC) at different stages of development across three continents. METHODS: Using the Centers for Disease Control and Prevention's (CDC's) International Registry Costing Tool (IntRegCosting Tool), cost and resource use data were collected from eight population-based cancer registries, including one in a low-income country (Uganda [Kampala)]), two in lower to middle-income countries (Kenya [Nairobi] and India [Mumbai]), and five in an upper to middle-income country (Colombia [Pasto, Barranquilla, Bucaramanga, Manizales and Cali cancer registries])...
October 25, 2016: Cancer Epidemiology
https://www.readbyqxmd.com/read/27782337/pentraxins-in-the-activation-and-regulation-of-innate-immunity
#16
REVIEW
Kenji Daigo, Antonio Inforzato, Isabella Barajon, Cecilia Garlanda, Barbara Bottazzi, Seppo Meri, Alberto Mantovani
Humoral fluid phase pattern recognition molecules (PRMs) are a key component of the activation and regulation of innate immunity. Humoral PRMs are diverse. We focused on the long pentraxin PTX3 as a paradigmatic example of fluid phase PRMs. PTX3 acts as a functional ancestor of antibodies and plays a non-redundant role in resistance against selected microbes in mouse and man and in the regulation of inflammation. This molecule interacts with complement components, thus modulating complement activation. In particular, PTX3 regulates complement-driven macrophage-mediated tumor progression, acting as an extrinsic oncosuppressor in preclinical models and selected human tumors...
November 2016: Immunological Reviews
https://www.readbyqxmd.com/read/27768027/nutrient-regulation-by-continuous-feeding-for-large-scale-expansion-of-mammalian-cells-in-spheroids
#17
Bradley P Weegman, Ahmad Essawy, Peter Nash, Alexandra L Carlson, Kristin J Voltzke, Zhaohui Geng, Marjan Jahani, Benjamin B Becker, Klearchos K Papas, Meri T Firpo
In this demonstration, spheroids formed from the β-TC6 insulinoma cell line were cultured as a model of manufacturing a mammalian islet cell product to demonstrate how regulating nutrient levels can improve cell yields. In previous studies, bioreactors facilitated increased culture volumes over static cultures, but no increase in cell yields were observed. Limitations in key nutrients such as glucose, which were consumed between batch feedings, can lead to limitations in cell expansion. Large fluctuations in glucose levels were observed, despite the increase in glucose concentrations in the media...
September 25, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27759806/red-blood-cells-in-cerebrospinal-fluid-as-possible-inhibitory-factor-for-enterovirus-rt-pcr
#18
Sérgio Monteiro de Almeida, Sônia Mara Raboni, Meri Bordignon Nogueira, Luine R Renaud Vidal
The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR...
October 2016: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/27759740/goal-directed-fluid-management-in-free-flap-surgery-for-cancer-of-the-head-and-neck
#19
Sanna L Lahtinen, Janne H Liisanantti, Meri M Poukkanen, Päivi A Laurila
BACKGROUND: Goal-directed fluid management using stroke volume variation (SVV) analysis is not well studied in free flap reconstruction surgery in patients with head and neck cancer. METHODS: Patients operated due to cancer of the head and neck with free flap reconstruction during 2008-2010 and 2012-2014 in Oulu University Hospital were retrospectively evaluated to determine the impact of SVV-guided fluid management on perioperative fluid balance, postoperative complications and hospital length of stay (LOS)...
October 19, 2016: Minerva Anestesiologica
https://www.readbyqxmd.com/read/27758135/development-and-validation-of-noninvasive-magnetic-resonance-relaxometry-for-the-in-vivo-assessment-of-tissue-engineered-graft-oxygenation
#20
Samuel A Einstein, Bradley P Weegman, Meri T Firpo, Klearchos K Papas, Michael Garwood
Techniques to monitor the oxygen partial pressure (pO2) within implanted tissue-engineered grafts (TEGs) are critically necessary for TEG development, but current methods are invasive and inaccurate. In this study, we developed an accurate and noninvasive technique to monitor TEG pO2 utilizing proton ((1)H) or fluorine ((19)F) magnetic resonance spectroscopy (MRS) relaxometry. The value of the spin-lattice relaxation rate constant (R1) of some biocompatible compounds is sensitive to dissolved oxygen (and temperature), while insensitive to other external factors...
November 2016: Tissue Engineering. Part C, Methods
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