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https://www.readbyqxmd.com/read/29691899/droplet-digital-pcr-for-bcr-abl-p210-detecting-of-cml-a-high-sensitive-method-of-the-minimal-residual-disease-disease-progression
#1
Wen-Jun Wang, Chao-Feng Zheng, Zhuang Liu, Yan-Hong Tan, Xiu-Hua Chen, Bin-Liang Zhao, Guo-Xia Li, Zhi-Fang Xu, Fang-Gang Ren, Yao-Fang Zhang, Jian-Mei Chang, Hong-Wei Wang
OBJECTIVE: The present study intended to establish a droplet digital PCR (dd-PCR) for monitoring minimal residual disease (MRD) in patients with BCR/ABL (P210)-positive CML, thereby achieving deep-level monitoring of tumor load and determining the efficacy for guided clinically individualized treatment. METHODS: Using dd-PCR and RT-qPCR, two cell suspensions were obtained from K562 cells and normal peripheral blood mononuclear cells by gradient dilution and were measured at the cellular level...
April 25, 2018: European Journal of Haematology
https://www.readbyqxmd.com/read/29691644/mir-101-3p-induces-autophagy-in-endometrial-carcinoma-cells-by-targeting-ezh2
#2
Cuilan Wang, Bo Liu
OBJECTIVE: This study aimed to investigate the effect of miR-101-3p on autophagy in endometrial carcinoma (EC) cells and the connection between miR-101-3p and EZH2. METHODS: The expression levels of miRNAs were analyzed by microarray. The expression level of autophagy related proteins was measured by western blot. The mRNA expression level of beclin-1 was determined by qRT-PCR. Autophagy in EC cells was traced by GFP-LC3 fusion protein and observed by fluorescence microscopy...
April 24, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29691299/undifferentiated-sarcomas-in-children-harbor-clinically-relevant-oncogenic-fusions-and-gene-copy-number-alterations-a-report-from-the-children-s-oncology-group
#3
Theodore W Laetsch, Angshumoy Roy, Lin Xu, Jennifer O Black, Cheryl M Coffin, Yueh-Yun Chi, Jing Tian, Sheri L Spunt, Douglas S Hawkins, Julia A Bridge, D Williams Parsons, Stephen X Skapek
PURPOSE: A comprehensive analysis of the genomics of undifferentiated sarcomas (UDS) is lacking.  We analyzed copy number alterations and fusion status in patients with UDS prospectively treated on Children's Oncology Group protocol ARST0332. EXPERIMENTAL DESIGN: Copy number alterations were assessed by Oncoscan FFPE Express on 32 UDS. Whole-exome and transcriptome libraries from 8 tumors with sufficient archived material were sequenced on HiSeq (2x100 bp). Targeted RNA-sequencing using Archer chemistry was performed on two additional cases...
April 24, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29691064/novel-ace2-fc-chimeric-fusion-provides-long-lasting-hypertension-control-and-organ-protection-in-mouse-models-of-systemic-renin-angiotensin-system-activation
#4
Pan Liu, Jan Wysocki, Tomokazu Souma, Minghao Ye, Veronica Ramirez, Bisheng Zhou, Lisa D Wilsbacher, Susan E Quaggin, Daniel Batlle, Jing Jin
Angiotensin-converting enzyme 2 (ACE2) is a carboxypeptidase that potently degrades angiotensin II to angiotensin 1-7. Previous studies showed that injection of the enzymatic ectodomain of recombinant ACE2 (rACE2) markedly increases circulatory levels of ACE2 activity, and effectively lowered blood pressure in angiotensin II-induced hypertension. However, due to the short plasma half-life of rACE2, its therapeutic potential for chronic use is limited. To circumvent this, we generated a chimeric fusion of rACE2 and the Ig fragment Fc segment to increase its plasma stability...
April 21, 2018: Kidney International
https://www.readbyqxmd.com/read/29690642/autophagic-removal-of-farnesylated-carboxy-terminal-lamin-peptides
#5
Xiang Lu, Karima Djabali
The mammalian nuclear lamina proteins—prelamin A- and B-type lamins—are post-translationally modified by farnesylation, endoproteolysis, and carboxymethylation at a carboxy-terminal CAAX (C, cysteine; a, aliphatic amino acid; X, any amino acid) motif. However, prelamin A processing into mature lamin A is a unique process because it results in the production of farnesylated and carboxymethylated peptides. In cells from patients with Hutchinson⁻Gilford progeria syndrome, the mutant prelamin A protein, progerin, cannot release its prenylated carboxyl-terminal moiety and therefore remains permanently associated with the nuclear envelope (NE), causing severe nuclear alterations and a dysmorphic morphology...
April 23, 2018: Cells
https://www.readbyqxmd.com/read/29690624/a-single-lidar-based-feature-fusion-indoor-localization-algorithm
#6
Yun-Ting Wang, Chao-Chung Peng, Ankit A Ravankar, Abhijeet Ravankar
In past years, there has been significant progress in the field of indoor robot localization. To precisely recover the position, the robots usually relies on multiple on-board sensors. Nevertheless, this affects the overall system cost and increases computation. In this research work, we considered a light detection and ranging (LiDAR) device as the only sensor for detecting surroundings and propose an efficient indoor localization algorithm. To attenuate the computation effort and preserve localization robustness, a weighted parallel iterative closed point (WP-ICP) with interpolation is presented...
April 23, 2018: Sensors
https://www.readbyqxmd.com/read/29689393/peak-timing-for-complications-following-adult-spinal-deformity-surgery
#7
Alan H Daniels, Shay Bess, Breton Line, Adam E M Eltorai, Daniel B C Reid, Virginie Lafage, Behrooz A Akbarnia, Christopher P Ames, Oheneba Boachie-Adjei, Douglas C Burton, Vedat Deviren, Han Jo Kim, Robert A Hart, Khaled M Kebaish, Eric O Klineberg, Munish Gupta, Gregory M Mundis, Richard A Hostin, Michael O'Brien, Frank J Schwab, Christopher I Shaffrey, Justin S Smith
BACKGROUND: Overall complication rates for adult spinal deformity (ASD) surgery have been reported, however little data exists on the peak timing associated with specific complications. This study quantifies the peak timing for multiple complication types in an ASD cohort at minimum 2-year follow up. METHODS: Multi-center, prospective analysis of all complications following ASD surgery in a consecutively enrolled cohort was performed. Inclusion criteria were ASD, age ≥ 18 years, spinal fusion ≥ 4 levels, and minimum 2-year follow-up...
April 21, 2018: World Neurosurgery
https://www.readbyqxmd.com/read/29689230/membrane-traffic-in-the-late-steps-of-cytokinesis
#8
REVIEW
Stéphane Frémont, Arnaud Echard
Cells don't simply separate at cytokinesis. While furrow contraction critically relies on myosin-II and F-actin, post-furrowing steps are less understood but involve the constriction of ESCRT-III polymer-dependent helices on the side of the midbody, which likely drive final abscission. The first evidence that animal cell cytokinesis requires membrane traffic, as in plant cells, was provided about 15 years ago. Since then, it has become increasingly clear that fusion of vesicles to the cytokinetic furrow is essential in large embryonic cells, and that membrane traffic within the intercellular bridge is crucial for its stability and successful abscission in all animal cells...
April 23, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29689198/grasp55-senses-glucose-deprivation-through-o-glcnacylation-to-promote-autophagosome-lysosome-fusion
#9
Xiaoyan Zhang, Leibin Wang, Behnam Lak, Jie Li, Eija Jokitalo, Yanzhuang Wang
The Golgi apparatus is the central hub for protein trafficking and glycosylation in the secretory pathway. However, how the Golgi responds to glucose deprivation is so far unknown. Here, we report that GRASP55, the Golgi stacking protein located in medial- and trans-Golgi cisternae, is O-GlcNAcylated by the O-GlcNAc transferase OGT under growth conditions. Glucose deprivation reduces GRASP55 O-GlcNAcylation. De-O-GlcNAcylated GRASP55 forms puncta outside of the Golgi area, which co-localize with autophagosomes and late endosomes/lysosomes...
April 23, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29689005/perioperative-complications-after-surgery-for-thoracic-ossification-of-posterior-longitudinal-ligament-nationwide-multicenter-prospective-study
#10
Shiro Imagama, Kei Ando, Kazuhiro Takeuchi, Satoshi Kato, Hideki Murakami, Toshimi Aizawa, Hiroshi Ozawa, Tomohiko Hasegawa, Yukihiro Matsuyama, Masao Koda, Masashi Yamazaki, Hirotaka Chikuda, Shigeo Shindo, Yukihiro Nakagawa, Atsushi Kimura, Katsushi Takeshita, Kanichiro Wada, Hiroyuki Katoh, Masahiko Watanabe, Kei Yamada, Takeo Furuya, Takashi Tsuji, Shunsuke Fujibayashi, Kanji Mori, Yoshiharu Kawaguchi, Kota Watanabe, Morio Matsumoto, Toshitaka Yoshii, Atsushi Okawa
STUDY DESIGN: Prospective, multicenter, nationwide study OBJECTIVE.: To investigate perioperative complications and risk factors in surgery for thoracic ossification of the posterior longitudinal ligament (T-OPLL) using data from the registry of the Japanese Multicenter Research Organization for Ossification of the Spinal Ligament. SUMMARY OF BACKGROUND DATA: There is no prospective multicenter study of surgical complications and risk factors for T-OPLL, and previous multicenter retrospective studies have lacked details...
April 23, 2018: Spine
https://www.readbyqxmd.com/read/29689004/assessment-of-proximal-junctional-kyphosis-and-shoulder-balance-with-proximal-screws-vs-hooks-in-posterior-spinal-fusion-for-adolescent-idiopathic-scoliosis
#11
Joshua M Pahys, Andrew C Vivas, Amer F Samdani, Gregory Cunn, Randal R Betz, Peter O Newton, Patrick J Cahill
STUDY DESIGN: A retrospective review of a prospectively collected multicenter database OBJECTIVE.: To assess the effect of proximal hooks vs. screws on proximal junctional kyphosis (PJK) as well as shoulder balance in otherwise all pedicle screw (>80%) posterior spinal fusion (PSF) constructs in adolescent idiopathic scoliosis (AIS). SUMMARY OF BACKGROUND DATA: Less rigid forms of fixation at the top of constructs in degenerative lumbar PSF have been postulated to decrease the risk of PJK...
April 23, 2018: Spine
https://www.readbyqxmd.com/read/29688069/development-of-kupffer-cell-targeting-type-i-interferon-for-the-treatment-of-hepatitis-via-inducing-anti-inflammatory-and-immunomodulatory-actions
#12
Yuki Minayoshi, Hitoshi Maeda, Hiroki Yanagisawa, Keisuke Hamasaki, Yuki Mizuta, Kento Nishida, Ryo Kinoshita, Yuki Enoki, Tadasi Imafuku, Victor Tuan Giam Chuang, Tomoaki Koga, Yukio Fujiwara, Motohiro Takeya, Kayoko Sonoda, Tomohiko Wakayama, Kazuaki Taguchi, Yu Ishima, Tatsuhiro Ishida, Yasuko Iwakiri, Motohiko Tanaka, Yutaka Sasaki, Hiroshi Watanabe, Masaki Otagiri, Toru Maruyama
Because of its multifaceted anti-inflammatory and immunomodulatory effects, delivering type-I interferon to Kupffer cells has the potential to function as a novel type of therapy for the treatment of various types of hepatitis. We report herein on the preparation of a Kupffer cell targeting type-I interferon, an albumin-IFNα2b fusion protein that contains highly mannosylated N-linked oligosaccharide chains, Man-HSA(D494N)-IFNα2b, attached by combining albumin fusion technology and site-directed mutagenesis...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29687234/ros-as-regulators-of-mitochondrial-dynamics-in-neurons
#13
REVIEW
Carolina Cid-Castro, Diego Rolando Hernández-Espinosa, Julio Morán
Mitochondrial dynamics is a complex process, which involves the fission and fusion of mitochondrial outer and inner membranes. These processes organize the mitochondrial size and morphology, as well as their localization throughout the cells. In the last two decades, it has become a spotlight due to their importance in the pathophysiological processes, particularly in neurological diseases. It is known that Drp1, mitofusin 1 and 2, and Opa1 constitute the core of proteins that coordinate this intricate and dynamic process...
April 23, 2018: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/29685991/1%C3%AE-25-oh-2-d-3-glycosides-from-solanum-glaucophyllum-leaves-extract-induce-myoblasts-differentiation-through-p38-mapk-and-akt-activation
#14
Ana Paula Irazoqui, Pablo De Genaro, Claudia Buitrago, Heinrich Bachmann, Verónica González-Pardo, Ana Russo de Boland
We have previously shown that Solanum glaucophyllum leaves extract (SGE) increases VDR protein levels and promotes myoblast differentiation. Here, we investigated whether p38 MAPK and AKT are involved in SGE actions. Cell cycle studies showed that SGE prompted a peak of S-phase followed by an arrest in the G0/G1-phase through p38 MAPK. Time course studies showed that p38 MAPK and AKT phosphorylation statically increased by SGE (10 nM) or synthetic 1α,25(OH)2 D3 (1 nM) treatment. Furthermore, p38 MAPK and AKT inhibitors, SB203580 and LY294002, respectively, suppressed myoblasts fusion induced by SGE or synthetic 1α,25(OH)2 D3 We have also studied differentiation genes by qRT-PCR...
April 23, 2018: Biology Open
https://www.readbyqxmd.com/read/29685981/angiotensin-1-7-reduces-cardiac-effects-of-thyroid-hormone-by-gsk3%C3%AE-nfatc3-signaling-pathway
#15
Nathalia Senger, Marcos Barrouin Melo, Gabriela Placoná Diniz, Maria José Campagnole-Santos, Robson Augusto Souza Dos Santos, Maria Luiza M Barreto-Chaves
Patients with hyperthyroidism exhibit increased risk of development and progression of cardiac diseases. The activation of the Renin Angiotensin System (RAS) has been indirectly implicated in these cardiac effects observed in hyperthyroidism. Angiotensin-(1-7) (Ang-(1-7)) has previously been shown to counterbalance pathological effects of Angiotensin II (Ang II). The aim of this study was to investigate the effects of elevated circulating Ang-(1-7) levels on cardiac effects promoted by hyperthyroidism in a transgenic rat (TG) model that constitutively overexpress an Ang-(1-7)-producing fusion protein [TGR(A1-7)3292]...
April 23, 2018: Clinical Science (1979-)
https://www.readbyqxmd.com/read/29685352/effects-of-n-glycosylation-on-the-biochemical-properties-of-recombinant-bek-l-expressed-in-pichia-pastoris
#16
Zhiyan Wang, Chao Guo, Lin Liu, He Huang
Enterokinase is an ideal tool protease for cleaving fusion proteins in genetic engineering. The bovine enterokinase light chain (bEKL ) produced in Pichia pastoris was shown to be a glycoprotein. To study the effects of N-glycosylation on the biochemical properties of bEKL , the enzyme was deglycosylated via site-directed mutagenesis. The results showed that elimination of the N-glycosylation sites of bEKL (N64, N103 and N165) did not significantly affect the protein secretion level in P. pastoris, but it does greatly influence its enzymatic activity...
July 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29685227/formation-of-high-order-oligomers-is-required-for-functional-bioactivity-of-an-african-bat-henipavirus-surface-glycoprotein
#17
Laura Behner, Louisa Zimmermann, Marc Ringel, Michael Weis, Andrea Maisner
Hendra virus (HeV) and Nipah virus (NiV) are highly pathogenic henipaviruses originating from fruit bats in Australia and Asia that can cause severe infections in livestock and humans. In recent years, also African bat henipaviruses were identified at the nucleic acid level. To assess their potential to replicate in non-bat species, several studies were performed to characterize the two surface glycoproteins required for virus entry and spread by cell-cell fusion. It has been shown that surface expression and fusion-helper function of the receptor-binding G protein of Kumasi virus (KV), the prototypic Ghanaian bat henipavirus, is reduced compared to other non-African henipavirus G proteins...
May 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29685100/comparative-genomics-of-the-wheat-fungal-pathogen-pyrenophora-tritici-repentis-reveals-chromosomal-variations-and-genome-plasticity
#18
Paula Moolhuijzen, Pao Theen See, James K Hane, Gongjun Shi, Zhaohui Liu, Richard P Oliver, Caroline S Moffat
BACKGROUND: Pyrenophora tritici-repentis (Ptr) is a necrotrophic fungal pathogen that causes the major wheat disease, tan spot. We set out to provide essential genomics-based resources in order to better understand the pathogenicity mechanisms of this important pathogen. RESULTS: Here, we present eight new Ptr isolate genomes, assembled and annotated; representing races 1, 2 and 5, and a new race. We report a high quality Ptr reference genome, sequenced by PacBio technology with Illumina paired-end data support and optical mapping...
April 23, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29684497/targeted-thermal-therapy-with-genetically-engineered-magnetite-magnetosomes-rgd-photothermia-is-far-more-efficient-than-magnetic-hyperthermia
#19
Anouchka Plan, Sandra Preveral, Alberto Curcio, Amanda Silva, Chistopher T Lefèvre, David Pignol, Yoann Lalatonne, Claire Wilhelm
Providing appropriate means for heat generation by low intratumoral nanoparticle concentrations is a major challenge for cancer nanotherapy. Here we propose RGD-tagged magnetosomes (magnetosomes@RGD) as a biogenic, genetically engineered, inorganic platform for multivalent thermal cancer treatment. Magnetosomes@RGD are biomagnetite nanoparticles synthesized by genetically modified magnetotactic bacteria thanks to a translational fusion of the RGD peptide with the magnetosomal protein MamC. Magnetosomes@RGD thus combine the high crystallinity of their magnetite core with efficient surface functionalization...
April 20, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29684441/cloning-and-characterization-of-apcystatin-a-plant-cystatin-gene-from-agapanthus-praecox-ssp-orientalis-responds-to-abiotic-stress
#20
Guan-Qun Chen, Di Zhang, Xiao-Hui Shen
Plant cystatins are involved in the regulation of protein turnover and play important roles in defense mechanisms. We cloned the ApCystatin gene from Agapanthus praecox ssp. orientalis, a famous ornamental and medical plant. The complete cDNA sequence of ApCystatin is comprised of 1446 nucleotides with a 420 bp ORF encoding 140 amino acids. The mRNA level of ApCystatin was significantly up-regulated under various abiotic stress, such as salt, osmosis, oxidative and cold stresses, which suggested that ApCystatin participated in the plant's resistance to stress...
April 20, 2018: Protein Expression and Purification
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