Aquaculture Salmon salar

Continuous artificial lighting (24LD) was introduced experimentally in the standard technology to grow salmon juveniles in a southern region (Republic of North Ossetia-Alania) and its effect on fatty acid (FA) composition of total lipids in muscles and the liver was assessed in parr and smolts of the Atlantic salmon Salmo salar L. Changes in the key FA were observed in the FA spectrum of fish, indicating that smoltification was complete and that smolts were ready for new habitat conditions. Thus the content of polyunsaturated FAs (PUFAs) significantly increased as a result of an increase in (n-3) PUFAs, and, in particular, marine-type 22:6(n-3), and high values were observed for (n-3)/(n-6) and 22:6(n-3)/18:3(n-3) PUFA ratios...

Methionine is an indispensable amino acid with an important role as the main methyl donor in cellular metabolism for both fish and mammals. Metabolization of methionine to the methyl donor S-adenosylmethionine (SAM) has consequence for polyamine, carnitine, phospholipid, and creatine synthesis as well as epigenetic modifications such as DNA- and histone tail methylation. Methionine can also be converted to cysteine and contributes as a precursor for taurine and glutathione synthesis. Moreover, methionine is the start codon for every protein being synthetized and thereby serves an important role in initiating translation...

Animal welfare assessments have struggled to investigate the emotional states of animals while focusing solely on available empirical evidence. Qualitative Behavioural Assessment (QBA) may provide insights into an animal's subjective experiences without compromising scientific rigor. Rather than assessing explicit, physical behaviours (i.e., what animals are doing, such as swimming or feeding), QBA describes and quantifies the overall expressive manner in which animals execute those behaviours (i.e., how relaxed or agitated they appear)...

Genome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available using this system, displaying differences regarding structure, cleavage, and PAM requirement. Cas9 is well established in Atlantic salmon, but Cas12a has yet to be tested in vivo in this species. In the present work, we microinjected salmon embryos with LbCas12a ribonucleoprotein complexes targeting the pigmentation gene solute carrier family 45 member 2 (slc45a2)...

Atlantic salmon ( Salmo salar ) is one of the worlds most domesticated fish. As production volumes increase, access to high quality and sustainable protein sources for formulated feeds of this carnivorous fish is required. Soybean meal (SBM) and soy-derived proteins are the dominant protein sources in commercial aquafeeds due to their low-cost, availability and favorable amino acid profile. However, for Atlantic salmon, the inclusion of soybean meal (SBM), and soy protein concentrate (SPC) in certain combinations can impact gut health, which has consequences for immunity and welfare, limiting the use of soy products in salmonid feeds...

Atlantic salmon (Salmo salar) possesses a genome containing 10 genes encoding chitinases, yet their functional roles remain poorly understood. In other fish species, chitinases have been primarily linked to digestion, but also to other functions, as chitinase-encoding genes are transcribed in a variety of non-digestive organs. In this study, we investigated the properties of two chitinases belonging to the family 18 glycoside hydrolase (GH18) group, namely Chia.3 and Chia.4, both isolated from the stomach mucosa...

Ulcer diseases are a recalcitrant issue at Atlantic salmon ( Salmo salar ) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa . However, several bacterial species are frequently isolated from ulcer disease events, and it is unclear if other undescribed pathogens are implicated in ulcer disease in Atlantic salmon. Although different polyvalent vaccines are used against M. viscosa , ulcerative outbreaks are continuously reported in Atlantic salmon in Canada...

Harmful algal blooms are one of the leading causes of mortality in salmon aquaculture, with significant economic consequences. From 15 to 31 October 1996, a bloom of the skeletonized form of Octactis speculum (Ehrenberg) F.H. Chang, J.M. Grieve & J.E. Sutherland was detected in the small Merexo inlet (1.7 km2 area), located on the southern shore of the Ria of Muxía (Galicia, NW Spain). The O. speculum population inside the inlet (data period: 1992-1996) seldom exceeded 4·103 cell·L-1 . However, its concentration reached 2·105 cell·L-1 during the bloom, coinciding with a decrease in light penetration from 5 to 2 m deep, as measured using a Secchi disk...

Atlantic salmon (Salmo salar) display high levels of agonistic behavior in aquaculture farms, resulting in fin damage and chronic stress. Aggression affects fish growth and performance negatively, and presents a serious welfare problem. Indeed, it would be beneficial to identify, separate or exclude overly aggressive individuals. Research on behavioral syndromes suggests that aggressive behavior may correlate with other behavioral traits, such as boldness and locomotory activity. We aimed to develop a high-throughput method to quantify and predict aggressive behavior of individual parr in hatchery-reared Baltic salmon (Salmo salar L...

BACKGROUND: Both bacterial and viral diseases are a major threat to farmed fish. As the antiviral immune mechanisms in lumpfish ( Cyclopterus lumpus L.) are poorly understood, lumpfish leukocytes were stimulated with poly(I:C), a synthetic analog of double stranded RNA, which mimic viral infections, and RNA sequencing was performed. METHODS: To address this gap, we stimulated lumpfish leukocytes with poly(I:C) for 6 and 24 hours and did RNA sequencing with three parallels per timepoint...

The effect of different feeding and lighting regimes (natural and continuous) on the phospholipid composition of Atlantic salmon fingerlings reared in commercial aquaculture in the summer-autumn period in North Ossetia-Alania was studied. Qualitative and quantitative determination of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, lysophosphatidylcholine, sphingomyelin was performed by high-performance liquid chromatography. A decrease (September-November) in the content of the studied phospholipids in fingerlings decreased, which should be considered primarily as a biochemical adaptation of development and preparation of juveniles for the upcoming smoltification...

Atlantic salmon (Salmo salar) in Northeastern US and Eastern Canada has high economic value for the sport fishing and aquaculture industries. Large differences exist between the genomes of Atlantic salmon of European origin and North American (N.A.) origin. Given the genetic and genomic differences between the two lineages, it is crucial to develop unique genomic resources for N.A. Atlantic salmon. Here we describe the resources that we recently developed for genomic and genetic research in N.A. Atlantic salmon aquaculture...

Piscirickettsiosis outbreaks due to Piscirickettsia salmonis occur globally in the Chilean salmon aquaculture generating significant monetary losses in the industry. P. salmonis secretes outer membrane vesicles (OMVs) which are naturally non-replicating and highly immunogenic spherical nanoparticles. P. salmonis OMVs has been shown to induce immune response in zebrafish; however, the immune response induced by these vesicles in salmonids has not been evaluated. In this study, we inoculated Atlantic salmon with 10 and 30 μg doses of P...

The negative genetic impacts of gene flow from domestic to wild populations can be dependent on the degree of domestication and exacerbated by the magnitude of pre-existing genetic differences between wild populations and the domestication source. Recent evidence of European ancestry within North American aquaculture Atlantic salmon (Salmo salar) has elevated the potential impact of escaped farmed salmon on often at-risk wild North American salmon populations. Here, we compare the ability of single nucleotide polymorphism (SNP) and microsatellite (SSR) marker panels of different sizes (7-SSR, 100-SSR and 220K-SNP) to detect introgression of European genetic information into North American wild and aquaculture populations...

Salmon aquaculture is constantly threatened by pathogens that impact fish health, welfare, and productivity, including the sea louse Caligus rogercresseyi . This marine ectoparasite is mainly controlled through delousing drug treatments that have lost efficacy. Therein, strategies such as salmon breeding selection represent a sustainable alternative to produce fish with resistance to sea lice. This study explored the whole-transcriptome changes in Atlantic salmon families with contrasting resistance phenotypes against lice infestation...

Rapid growth in aquaculture has resulted in high-density production systems in ecologically and geographically novel conditions in which the emergence of diseases is inevitable. Well-characterized methods for detection and surveillance of infectious diseases are vital for rapid identification, response, and recovery to protect economic and food security. We implemented a proof-of-concept approach for virus detection using a known high-consequence fish pathogen, infectious salmon anemia virus (ISAV), as the archetypal pathogen...

The molecular repertoire of the mucosa-associated lymphoid tissue (MALT) in the olfactory rosette in most teleost fish is unknown. Here we present the basal transcriptome of the olfactory rosette of Atlantic salmon (Salmo salar). To investigate its mucosal immune features, we performed a comparative transcriptomic analysis with the gills, one of the most studied organs possessing MALT. Pathway enrichment revealed that cytokine-cytokine interaction and the neuroactive ligand-receptor interaction pathways were at the core of the shared similarity between the two organs...

The production of farmed Atlantic salmon (Salmo salar) is currently linked to finite marine resources, particularly fish oil (FO) and fish meal (FM). Understanding this relationship in a quantitative manner is imperative if this critical balance is to be maintained within sustainable limits as the industry grows. Here we project the potential production and associated growth of the Atlantic salmon aquaculture industry on the basis of a variety of FO and FM utilization scenarios in aquafeed. Reducing FO and FM dietary inclusion to 3% each could permit production growth of 2% per year until the turn of the century (2097 and beyond 2100, respectively), independent of a host of alternatives now being utilized-with three portions of salmon per week providing almost all the recommended weekly long-chain omega-3 fatty acids for human intake...

Given the hydrophilic structure of lysophospholipids (LPLs), their dietary inclusion translates into a better emulsifying capacity of the dietary components. The present study aimed to understand the mechanisms underlying the growth-promoting effect of LPL supplementation by undertaking deep analyses of the proximal intestine and liver interactomes. The Atlantic salmon ( Salmo salar ) was selected as the main aquaculture species model. The animals were divided into two groups: one was fed a control diet (C-diet) and the other a feed (LPL-diet) supplemented with an LPL-based digestive enhancer (0...

It remains a challenge to obtain the desired phenotypic traits in aquacultural production of Atlantic salmon, and part of the challenge might come from the effect that host-associated microorganisms have on the fish phenotype. To manipulate the microbiota towards the desired host traits, it is critical to understand the factors that shape it. The bacterial gut microbiota composition can vary greatly among fish, even when reared in the same closed system. While such microbiota differences can be linked to diseases, the molecular effect of disease on host-microbiota interactions and the potential involvement of epigenetic factors remain largely unknown...