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https://www.readbyqxmd.com/read/29786935/evaluation-of-pcr-primers-targeting-the-groel-gene-for-the-specific-detection-of-streptococcus-agalactiae-in-the-context-of-aquaculture
#1
W J Leigh, R N Zadoks, A Jaglarz, J Z Costa, G Foster, K D Thompson
AIMS: The aim of this study was to design a set of primers for specific detection and identification of Streptococcus agalactiae in polymerase chain reaction (PCR) that can detect a diverse range of S. agalactiae isolates from different hosts and that it is capable of discriminating between S. agalactiae and other species that are closely related or potentially present in aquaculture environments, notably Streptococcus iniae. METHODS AND RESULTS: Primers, based on the groEL2 gene of S...
May 22, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29785397/whole-genome-sequencing-of-greater-amberjack-seriola-dumerili-for-snp-identification-on-aligned-scaffolds-and-genome-structural-variation-analysis-using-parallel-resequencing
#2
Kazuo Araki, Jun-Ya Aokic, Junya Kawase, Kazuhisa Hamada, Akiyuki Ozaki, Hiroshi Fujimoto, Ikki Yamamoto, Hironori Usuki
Greater amberjack ( Seriola dumerili ) is distributed in tropical and temperate waters worldwide and is an important aquaculture fish. We carried out de novo sequencing of the greater amberjack genome to construct a reference genome sequence to identify single nucleotide polymorphisms (SNPs) for breeding amberjack by marker-assisted or gene-assisted selection as well as to identify functional genes for biological traits. We obtained 200 times coverage and constructed a high-quality genome assembly using next generation sequencing technology...
2018: International Journal of Genomics
https://www.readbyqxmd.com/read/29785396/expression-characterization-of-six-genes-possibly-involved-in-gonad-development-for-stellate-sturgeon-individuals-acipenser-stellatus-pallas-1771
#3
Alexandru Burcea, Gina-Oana Popa, Iulia Elena Florescu Gune, Marilena Maereanu, Andreea Dudu, Sergiu Emil Georgescu, Marieta Costache
Nowadays, in sturgeon's aquaculture, there is a necessity for sex identification at early stages in order to increase the efficiency of this commercial activity. The basis for a correct identification is studying the different factors that influence the gonad development. The research has been directed towards molecular methods that have been employed with various degrees of success in identifying genes with different expression patterns between male and female sturgeons during their development stages. For the purpose of understanding the sexual development of 4-year-old stellate sturgeon (Acipenser stellatus) individuals, we have selected six genes ( foxl2 , cyp17a1 , ar , dmrt1 , sox9 , and star )...
2018: International Journal of Genomics
https://www.readbyqxmd.com/read/29780377/the-gut-microbiota-of-marine-fish
#4
REVIEW
Sian Egerton, Sarah Culloty, Jason Whooley, Catherine Stanton, R Paul Ross
The body of work relating to the gut microbiota of fish is dwarfed by that on humans and mammals. However, it is a field that has had historical interest and has grown significantly along with the expansion of the aquaculture industry and developments in microbiome research. Research is now moving quickly in this field. Much recent focus has been on nutritional manipulation and modification of the gut microbiota to meet the needs of fish farming, while trying to maintain host health and welfare. However, the diversity amongst fish means that baseline data from wild fish and a clear understanding of the role that specific gut microbiota play is still lacking...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29780359/the-mitotic-and-metabolic-effects-of-phosphatidic-acid-in-the-primary-muscle-cells-of-turbot-scophthalmus-maximus
#5
Tingting Wang, Xuan Wang, Huihui Zhou, Haowen Jiang, Kangsen Mai, Gen He
Searching for nutraceuticals and understanding the underlying mechanism that promote fish growth is at high demand for aquaculture industry. In this study, the modulatory effects of soy phosphatidic acids (PA) on cell proliferation, nutrient sensing, and metabolic pathways were systematically examined in primary muscle cells of turbot ( Scophthalmus maximus ). PA was found to stimulate cell proliferation and promote G1/S phase transition through activation of target of rapamycin signaling pathway. The expression of myogenic regulatory factors, including myoD and follistatin , was upregulated, while that of myogenin and myostatin was downregulated by PA...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29778844/identification-of-micrornas-with-heat-stress-responsive-and-immune-properties-in-marsupenaeus-japonicus-based-on-next-generation-sequencing-and-bioinformatics-analysis-essential-regulators-in-the-heat-stress-host-interactions
#6
Jinbin Zheng, Jiawen Cao, Yong Mao, Yongquan Su, Jun Wang
Summer mortality syndrome is one of the most serious issue for Marsupenaeus japonicus aquaculture in China. Since it causes massive economic loss and threatens sustainability of M. japonicus aquaculture industry, thus, there is an urgent desire to reveal the heat stress-host interactions mechanisms that lead to mass mortalities of M. japonicus in hot summer months. MicroRNAs (miRNAs) are small noncoding RNAs that involved in regulation of diverse biological processes, including stress and immune response, and might serve as potential regulators in the heat stress-host interactions...
May 17, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29778226/imbalanced-nutrient-regimes-increase-prymnesium-parvum-resilience-to-herbicide-exposure
#7
Stacie L Flood, JoAnn M Burkholder
The toxigenic haptophyte Prymnesium parvum is a mixotrophic phytoplankter with an extensive historic record of forming nearly monospecific, high-biomass, ecosystem-disrupting blooms, and it has been responsible for major fish kills in brackish waters and aquaculture facilities in many regions of the world. Little is known about how this species responds to commonly occurring environmental contaminants, or how nutrient (nitrogen, phosphorus) pollution may interact with environmentally relevant pesticide exposures to affect this harmful algal species...
May 2018: Harmful Algae
https://www.readbyqxmd.com/read/29778220/development-of-a-qpcr-assay-to-detect-and-quantify-ichthyotoxic-flagellates-along-the-norwegian-coast-and-the-first-norwegian-record-of-fibrocapsa-japonica-raphidophyceae
#8
Anette Engesmo, David Strand, Sandra Gran-Stadniczeñko, Bente Edvardsen, Linda K Medlin, Wenche Eikrem
Blooms of ichthyotoxic microalgae pose a great challenge to the aquaculture industry world-wide, and there is a need for fast and specific methods for their detection and quantification in monitoring programs. In this study, quantitative real-time PCR (qPCR) assays for the detection and enumeration of three ichthyotoxic flagellates: the dinoflagellate Karenia mikimotoi (Miyake & Kominami ex Oda) Hansen & Moestrup and the two raphidophytes Heterosigma akashiwo (Hada) Hada ex Hara & Chihara and Fibrocapsa japonica Toriumi & Takano were developed...
May 2018: Harmful Algae
https://www.readbyqxmd.com/read/29777417/effects-of-dietary-microencapsulated-cymbopogon-flexuosus-essential-oil-on-reproductive-related-parameters-in-male-rhamdia-quelen
#9
Carine de Freitas Souza, Cristine Rampelotto, Bruno Bianchi Loureiro, Fernanda Alves Pereira, Adriane Erbice Bianchini, Carine Dahl Corcini, Antonio Sergio Varela Junior, Tatiana Emanuelli, Leila Picolli da Silva, Sílvio Teixeira da Costa, Kalyne Bertolin, Monique Tomazele Rovani, Paulo Bayard Dias Gonçalves, Berta Maria Heinzmann, Bernardo Baldisserotto
In aquaculture, nutrition and supplemented diets have been shown to affect broodstock reproductive performance. In this study, we investigated the effects of dietary supplementation with Cymbopogon flexuosus essential oil (CFEO) microcapsules on reproductive-related parameters in silver catfish (Rhamdia quelen) male broodfish. Adult male broodstocks were separated into three groups according to the concentrations of supplemented CFEO (0.0 = control; 1.0 or 3.0 mL per kg of diet). After 20 days under experimental conditions, the animals were euthanized and the gonads were harvested for gonadosomatic index, sperm analysis, oxidative stress, and histopathology; testosterone levels were measured in the plasma; gene expression of prl, smtl, pomca, and pomcb was assessed in the pituitary gland by real-time PCR...
May 18, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29777415/preparation-of-biologically-active-monomeric-recombinant-zebrafish-danio-rerio-and-rainbow-trout-oncorhynchus-mykiss-recombinant-growth-hormones
#10
Ewa Ocłoń, Gili Solomon, Zvi Hayouka, Arieh Gertler
Fish growth hormones (GHs) play an important role in regulating growth, metabolism, reproduction, osmoregulation, and immunity and have thus garnered attention for their application in aquaculture. Zebrafish GH (zGH) cDNA or rainbow trout GH (rtGH) cDNA was cloned into the pMon3401 vector, expressed in MON105-competent Escherichia coli and purified to homogeneity. Their biological activity was evidenced by their ability to interact with ovine GH receptor extracellular domain and stimulate GH receptor-mediated proliferation in FDC-P1-3B9 cells stably transfected with rabbit GH receptor...
May 18, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29776453/dye-residue-analysis-in-raw-and-processed-aquaculture-products-matrix-extension-of-aoac-international-official-method-%C3%A2-2012-25
#11
Wendy C Andersen, Christine R Casey, Tara J Nickel, Susan L Young, Sherri B Turnipseed
Background : Triphenylmethane dyes and metabolites are known or suspected mutagens and areprohibited in animals intended for human consumption. Despite toxicity, triphenylmethane dyes are used illegally as inexpensive treatments for fungal and parasite infections in aquatic animals. Objective: AOAC INTERNTIONAL Official Method ℠ 2012.25 for the LC-MS/MS determination of malachite green, crystal violet, brilliant green, and metabolites leucomalachite green and leucocrystal violet in seafood products was previously validated for finfish (trout, salmon, catfish, and tilapia) and shrimp, but had not been fully validated for other types of aquacultured products such as eel, molluscan shellfish, or frog or for processed seafoods...
May 18, 2018: Journal of AOAC International
https://www.readbyqxmd.com/read/29775727/isolation-structure-elucidation-and-antibacterial-activity-of-methyl-4-8-dimethylundecanate-from-the-marine-actinobacterium-streptomyces-albogriseolus-ecr64
#12
Durairaj Thirumurugan, Ramasamy Vijayakumar, Chithravel Vadivalagan, Pushparaj Karthika, Md Khurshid Alam Khan
Around 120 actinobacterial colonies were isolated from various regions of marine East coast region of Tamil Nadu, India. Among them, 33 were morphologically distinct and they were preliminarily screened for their antibacterial activity against Pseudomonas fluorescens, Vibrio cholerae, V. parahaemolyticus, V. alginolyticus, and Aeromonas hydrophila by cross-streak plate technique. Among the isolated, the isolate ECR64 exhibited maximum zone of inhibition against fish pathogenic bacteria. The crude bioactive compounds were extracted from the isolate ECR64 using different organic solvents which exhibited maximum antibacterial activity...
May 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29772736/exploring-the-effect-of-phage-therapy-in-preventing-vibrio-anguillarum-infections-in-cod-and-turbot-larvae
#13
Nanna Rørbo, Anita Rønneseth, Panos G Kalatzis, Bastian Barker Rasmussen, Kirsten Engell-Sørensen, Hans Petter Kleppen, Heidrun Inger Wergeland, Lone Gram, Mathias Middelboe
The aquaculture industry is suffering from losses associated with bacterial infections by opportunistic pathogens. Vibrio anguillarum is one of the most important pathogens, causing vibriosis in fish and shellfish cultures leading to high mortalities and economic losses. Bacterial resistance to antibiotics and inefficient vaccination at the larval stage of fish emphasizes the need for novel approaches, and phage therapy for controlling Vibrio pathogens has gained interest in the past few years. In this study, we examined the potential of the broad-host-range phage KVP40 to control four different V...
May 16, 2018: Antibiotics
https://www.readbyqxmd.com/read/29772529/microbial-bio-fuels-a-solution-to-carbon-emissions-and-energy-crisis
#14
Arun Kumar, Sumit Kaushal, Shubhini A Saraf, Jay Shankar Singh
Increasing energy demand, limited fossil fuel resources and climate change have prompted development of alternative sustainable and economical fuel resources such as crop-based bio-ethanol and bio-diesel. However, there is concern over use of arable land that is used for food agriculture for creation of biofuel. Thus, there is a renewed interest in the use of microbes particularly microalgae for bio-fuel production. Microbes such as micro-algae and cyanobacteria that are used for biofuel production also produce other bioactive compounds under stressed conditions...
June 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/29770449/effects-of-a-nanoemulsion-with-copaifera-officinalis-oleoresin-against-monogenean-parasites-of-colossoma-macropomum-a-neotropical-serrasalmidae
#15
D S S Valentim, J L Duarte, A E M F M Oliveira, R A S Cruz, J C T Carvalho, C Solans, C P Fernandes, M Tavares-Dias
Monogeneans are ectoparasites that may cause losses in production and productivity in the aquaculture of Colossoma macropomum. Chemotherapeutics used in aquaculture usually have major adverse effects on fish; hence, the use of essential oils has been considered advantageous, but these are not soluble in water. Thus, the use of nanostructures to enhance water solubility of compounds and improve bioactivity may be very promising. This study investigated the antiparasitic activity of nanoemulsion prepared with Copaifera officinalis oleoresin (50, 100, 150, 200 and 300 mg/L), against monogenean parasites from the gills of C...
May 16, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29767119/recent-advances-in-sturgeon-nutrition
#16
REVIEW
Silas S O Hung
Sturgeons are fish species of biological and economical importance, and most of them are endangered, vulnerable or rare because of their large size, late sexual maturity, long period between spawning, and longevity. These unique biological characteristics make them highly susceptible to overfishing, degradation of habitat and spawning ground, and contamination of water and sediments by pollutants. The objective of the current review is not to exhaustively include all studies on sturgeon nutrient requirements and utilizations conducted under laboratory conditions, but to critique some studies and update previous reviews...
September 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766417/the-fto-gene-is-associated-with-growth-and-omega-3-6-ratio-in-asian-seabass
#17
Fei Sun, Rongjian Tu, Jun Hong Xia, Xiao Jun Liu, Gen Hua Yue
Polymorphisms in the FTO gene are associated with obesity and body mass index in humans and livestock. Little information of whether FTO plays an important role in aquaculture fish species is available. We cloned and characterized the FTO gene in an economically important food fish species: Asian seabass (Lates calcarifer). The full-length cDNA of the gene is 3679 bp, containing an ORF of 1935 bp encoding 644 amino acids, a 216 bp 5' UTR and a 1538 bp 3' UTR. The gene consisted of nine exons and eight introns and was 117,679 bp in length...
May 16, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29765621/biofilm-inhibition-and-pathogenicity-attenuation-in-bacteria-by-proteus-mirabilis
#18
Shichen Yu, Xiaoshan Zhu, Jin Zhou, Zhonghua Cai
Biofilms play an important role in the antibiotic resistance of encased bacteria, and biofilm formation is regulated by quorum sensing (QS). Inhibiting the QS system may, therefore, degrade the integrity of a biofilm and expose the bacterial pathogens within it to the deleterious effects of molecules such as antibiotics. Moreover, the use of QS inhibitors (QSIs) may provide a novel approach for treating bacterial infections of aquacultures. In the present study, the bacterium Proteus mirabilis was identified as a potential producer of QSIs...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29765364/rearing-water-treatment-induces-microbial-selection-influencing-the-microbiota-and-pathogen-associated-transcripts-of-cod-gadus-morhua-larvae
#19
Ragnhild I Vestrum, Kari J K Attramadal, Per Winge, Keshuai Li, Yngvar Olsen, Atle M Bones, Olav Vadstein, Ingrid Bakke
We have previously shown that K-selection and microbial stability in the rearing water increases survival and growth of Atlantic cod ( Gadus morhua ) larvae, and that recirculating aquaculture systems (RAS) are compatible with this. Here, we have assessed how water treatment influenced the larval microbiota and host responses at the gene expression level. Cod larvae were reared with two different rearing water systems: a RAS and a flow-through system (FTS). The water microbiota was examined using a 16S rDNA PCR/DGGE strategy...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29764681/plasmid-pcdna3-1-s11-constructed-based-on-the-s11-segment-of-grass-carp-reovirus-as-dna-vaccine-provides-immune-protection
#20
Yan Gao, Chao Pei, Xiaoying Sun, Chao Zhang, Li Li, Xianghui Kong
Although some commercial vaccines against grass carp reovirus (GCRV) are available, given the many varieties of GCRV and limited types of vaccines, the disease caused by GCRV remains a major problem, which leads to economic losses in grass carp aquaculture. A reovirus strain (GCRV-HN14) was recently isolated from local diseased fish in our laboratory. The S11 segment of GCRV-HN14 was speculated to encode the virus capsid protein VP35. In our study, the S11 segment was cloned into the eukaryotic expression vector pcDNA3...
May 12, 2018: Vaccine
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