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https://www.readbyqxmd.com/read/28937880/detection-of-free-microcystins-in-the-liver-and-muscle-of-freshwater-fish-by-liquid-chromatography-tandem-mass-spectrometry
#1
Xiaobin Hu, Jinyun Ye, Rongfei Zhang, Xiang Wu, Yixiang Zhang, Chenglong Wu
MC analysis of biological tissue is considered to be very difficult due to the lack of validated methods. This is the primary limiting factor for monitoring potential risks in both the flesh of aquatic organisms and the aquatic ecosystem. In this study, an effective method to determine free MCs (MC-LR and MC-RR) in the muscle and liver tissues of freshwater cultured fish was developed using solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC/MS-MS). The extraction solvent, time of extraction, eluent and purification of the extract were optimized...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937591/metabolic-engineering-of-escherichia-coli-for-producing-astaxanthin-as-the-predominant-carotenoid
#2
Qian Lu, Yi-Fan Bu, Jian-Zhong Liu
Astaxanthin is a carotenoid of significant commercial value due to its superior antioxidant potential and wide applications in the aquaculture, food, cosmetic and pharmaceutical industries. A higher ratio of astaxanthin to the total carotenoids is required for efficient astaxanthin production. β-Carotene ketolase and hydroxylase play important roles in astaxanthin production. We first compared the conversion efficiency to astaxanthin in several β-carotene ketolases from Brevundimonas sp. SD212, Sphingomonas sp...
September 22, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28936125/vitrification-as-an-alternative-approach-for-sperm-cryopreservation-in-marine-fishes
#3
Rafael Cuevas-Uribe, E Hu, Harry Daniels, Adriane O Gill, Terrence R Tiersch
The Southern Flounder Paralichthys lethostigma is a high-value species and a promising aquaculture candidate. Because sperm volume can be limited in this species (<500 μL), new sperm cryopreservation methods need to be evaluated. Vitrification is an alternative to conventional slow-rate freezing, whereby small volumes are cryopreserved at high cooling rates (>1,000°C/min). The goal of this work was to develop a standardized approach for vitrification of Southern Flounder sperm. The specific objectives were to (1) evaluate thawing methods and vitrification solutions, (2) evaluate the postthaw membrane integrity of sperm vitrified in different cryoprotectant solutions, (3) examine the relationship between membrane integrity and motility, and (4) evaluate the ability of vitrified sperm to fertilize eggs...
2017: North American Journal of Aquaculture
https://www.readbyqxmd.com/read/28935875/differential-gene-expression-and-snp-association-between-fast-and-slow-growing-turbot-scophthalmus-maximus
#4
Diego Robledo, Juan A Rubiolo, Santiago Cabaleiro, Paulino Martínez, Carmen Bouza
Growth is among the most important traits for animal breeding. Understanding the mechanisms underlying growth differences between individuals can contribute to improving growth rates through more efficient breeding schemes. Here, we report a transcriptomic study in muscle and brain of fast- and slow-growing turbot (Scophthalmus maximus), a relevant flatfish in European and Asian aquaculture. Gene expression and allelic association between the two groups were explored. Up-regulation of the anaerobic glycolytic pathway in the muscle of fast-growing fish was observed, indicating a higher metabolic rate of white muscle...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28934685/use-of-food-waste-fish-waste-and-food-processing-waste-for-china-s-aquaculture-industry-needs-and-challenge
#5
Wing Yin Mo, Yu Bon Man, Ming Hung Wong
China's aquaculture industry is growing dramatically in recent years and now accounts for 60.5% of global aquaculture production. Fish protein is expected to play an important role in China's food security. Formulated feed has become the main diet of farmed fish. The species farmed have been diversified, and a large amount of 'trash fish' is directly used as feed or is processed into fishmeal for fish feed. The use of locally available food waste as an alternative protein source for producing fish feed has been suggested as a means of tackling the problem of sourcing safe and sustainable feed...
September 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28931084/barcoding-snakeheads-teleostei-channidae-revisited-discovering-greater-species-diversity-and-resolving-perpetuated-taxonomic-confusions
#6
Cecilia Conte-Grand, Ralf Britz, Neelesh Dahanukar, Rajeev Raghavan, Rohan Pethiyagoda, Heok Hui Tan, Renny K Hadiaty, Norsham S Yaakob, Lukas Rüber
Snakehead fishes of the family Channidae are predatory freshwater teleosts from Africa and Asia comprising 38 valid species. Snakeheads are important food fishes (aquaculture, live food trade) and have been introduced widely with several species becoming highly invasive. A channid barcode library was recently assembled by Serrao and co-workers to better detect and identify potential and established invasive snakehead species outside their native range. Comparing our own recent phylogenetic results of this taxonomically confusing group with those previously reported revealed several inconsistencies that prompted us to expand and improve on previous studies...
2017: PloS One
https://www.readbyqxmd.com/read/28930088/comparison-of-pcr-methods-for-the-detection-of-genetic-variants-of-carp-edema-virus
#7
Mikolaj Adamek, Marek Matras, Verena Jung-Schroers, Felix Teitge, Max Heling, Sven M Bergmann, Michal Reichert, Keith Way, David M Stone, Dieter Steinhagen
The infection of common carp and its ornamental variety, koi, with the carp edema virus (CEV) is often associated with the occurrence of a clinical disease called 'koi sleepy disease'. The disease may lead to high mortality in both koi and common carp populations. To prevent further spread of the infection and the disease, a reliable detection method for this virus is required. However, the high genetic variability of the CEV p4a gene used for PCR-based diagnostics could be a serious obstacle for successful and reliable detection of virus infection in field samples...
September 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28930084/high-nervous-necrosis-virus-nnv-diversity-in-wild-wrasse-labridae-in-norway-and-sweden
#8
Kjetil Korsnes, Egil Karlsbakk, Cecilie K Skår, Lisbeth Sælemyr, Are Nylund, Bjørn Olav Kvamme, Stein Mortensen
Wild goldsinny wrasse Ctenolabrus rupestris, corkwing wrasse Symphodus melops and ballan wrasse Labrus bergylta were collected at 8 sampling sites in Sweden and Norway during summer 2014. Brain tissue from 466 wrasses were analyzed for nervous necrosis virus (NNV) infections by real-time RT-PCR, and positive samples were subjected to sequencing and phylogenetic analysis of partial segments of the RNA2 and RNA1 genes. This study shows that NNV is present in wild ballan, corkwing and goldsinny wrasse along the coastline of Sweden and Norway...
September 20, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28929986/the-effect-of-temperature-on-the-initial-development-of-brycon-amazonicus-spix-agassiz-1829-as-tool-for-micromanipulation-of-embryos
#9
Regiane Cristina da Silva, Matheus Pereira Dos Santos, José Augusto Senhorini, Maria do Carmo Faria Paes, Fernanda Nogueira Valentin, Takafumi Fujimoto, Nivaldo Ferreira do Nascimento, George Shigueki Yasui, Laura Satiko Okada Nakaghi
Primordial germ cell (PGC) transplant is a promising tool in aquaculture; however, successful use of this technique requires in depth knowledge of the early stages of embryo and larval development. The aim of this study was to analyse the effect of different temperatures (22, 26, and 30°C) on the early development of B. amazonicus. The newly fertilized eggs were distributed into tanks with controlled temperature and oxygenation. Samples were collected at pre-established times and analysed under light and fluorescence microscopy...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28929406/pharmacopollution-and-household-waste-medicine-hwm-how-reverse-logistics-is-environmentally-important-to-brazil
#10
André Luiz Pereira, Raphael Tobias de Vasconcelos Barros, Sandra Rosa Pereira
Pharmacopollution is a public health and environmental outcome of some active pharmaceutical ingredients (API) and endocrine-disrupting compounds (EDC) dispersed through water and/or soil. Its most important sources are the pharmaceutical industry, healthcare facilities (e.g., hospitals), livestock, aquaculture, and households (patients' excretion and littering). The last source is the focus of this article. Research questions are "What is the Household Waste Medicine (HWM) phenomenon?", "How HWM and pharmacopollution are related?", and "Why is a reverse logistic system necessary for HWM in Brazil?" This article followed the seven steps proposed by Rother (2007) for a systematic review based on the Cochrane Handbook and the National Health Service (NHS) Center for Reviews Dissemination (CDR) Report...
September 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28927687/transcriptome-analysis-of-the-spleen-of-the-darkbarbel-catfish-pelteobagrus-vachellii-in-response-to-aeromonas-hydrophila-infection
#11
Chuanjie Qin, Quan Gong, Zhengyong Wen, Dengyue Yuan, Ting Shao, Jun Wang, Huatao Li
Intensive aquaculture has increased the susceptibility of fish to Aeromonas hydrophila, and this has led to severe economic damage. There has been little study of the host defense mechanism against A. hydrophila infection in scaleless fish. Therefore, in the present study, the transcriptome profiles of the spleen of Pelteobagrus vachellii were examined after infection with A. hydrophila. In total, 37,730 unigenes from 322 KEGG pathways were identified. Following A. hydrophila infection, 27,803 differentially expressed genes were identified, including 13,934 upregulated and 13,869 downregulated genes...
September 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28926005/social-and-economic-aspects-of-aquatic-animal-health
#12
K E Adam, G J Gunn
Aquaculture is an increasingly important source of animal protein for a growing global population. Disease is a major constraint to production, with resultant socio-economic impacts for individuals, communities and economies which rely on aquaculture. Aquatic animal health is also strongly influenced by human factors, ranging from international trade regulations to the behaviours of individuals working in aquaculture. This article summarises the human factors associated with aquaculture production using international examples for illustration...
April 2017: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/28924190/microbiome-of-pacific-whiteleg-shrimp-reveals-differential-bacterial-community-composition-between-wild-aquacultured-and-ahpnd-ems-outbreak-conditions
#13
Fernanda Cornejo-Granados, Alonso A Lopez-Zavala, Luigui Gallardo-Becerra, Alfredo Mendoza-Vargas, Filiberto Sánchez, Rodrigo Vichido, Luis G Brieba, Maria Teresa Viana, Rogerio R Sotelo-Mundo, Adrián Ochoa-Leyva
Crustaceans form the second largest subphylum on Earth, which includes Litopeneaus vannamei (Pacific whiteleg shrimp), one of the most cultured shrimp worldwide. Despite efforts to study the shrimp microbiota, little is known about it from shrimp obtained from the open sea and the role that aquaculture plays in microbiota remodeling. Here, the microbiota from the hepatopancreas and intestine of wild type (wt) and aquacultured whiteleg shrimp and pond sediment from hatcheries were characterized using sequencing of seven hypervariable regions of the 16S rRNA gene...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923608/molecular-characterization-and-phylogenetic-analysis-of-highly-pathogenic-vibrio-alginolyticus-strains-isolated-during-mortality-outbreaks-in-cultured-ruditapes-decussatus-juvenile
#14
Badreddine Mechri, Monastiri Abir, Amel Medhioub, Mohamed Nejib Medhioub, Mahjoub Aouni
In the summer of 2008 and 2009, a series of mortalities in growing out seeds of R. decussatus juveniles were occurred in the eastern Tunisian littoral. Nine predominant bacterial strains were isolated from dead and moribund juveniles and characterized as Vibrio alginolyticus. These isolates were subjected to biochemical and molecular characterization. All the Vibrio strains were tested for their susceptibility against the most widely used antibiotic in aquaculture as well as, the assessment of the presence of erythromycin (emrB) and tetracycline (tetS) resistance genes among the tested bacteria...
September 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28923565/chitin-and-chitosan-preparation-from-shrimp-shells-penaeus-monodon-and-its-human-ovarian-cancer-cell-line-pa-1
#15
Haripriya Srinivasan, Kanayairam Velayutham, Ramanibai Ravichandran
In the present study, chitin and chitosan preparation from shrimp shells Penaeus mondon and its ovarian cancer cell line (PA-1). FTIR spectrum sharp absorption peak at 1655cm(-1) is assigned to ketone C=O (α) unsaturated with chitosan. X-ray diffraction showed the presence of chitin and chitosan were strongest peak at 18.91° (β) and 29.75° (α) characters. SEM observations of chitin and chitosan surface morphologies of P. monodon showed that microfibril and porous structures. Anticancer activity of chitin and chitosan against human ovarian cancer cell line showed that chitosan an exhibited notable higher activity than chitin...
September 15, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28919449/genome-editing-in-fishes-and-their-applications
#16
Bo Zhu, Wei Ge
There have been revolutionary progresses in genome engineering in the past few years. The newly-emerged genome editing technologies including zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeats associated with Cas9 (CRISPR/Cas9) have enabled biological scientists to perform efficient and precise targeted genome editing in different species. Fish represent the largest group of vertebrates with many species having values for both scientific research and aquaculture industry...
September 14, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28919178/the-presence-of-abalone-egg-laying-hormone-like-peptide-in-the-central-nervous-system-and-ovary-of-the-spotted-babylon-babylonia-areolata
#17
Jirawat Saetan, Piyakorn Boonyoung, Uraporn Vongvatcharanon, Thanapong Kruangkum, Kanjana Khornchatri, Pinij Thaweethamsewee, Prasert Sobhon, Prapee Sretarugsa
Recently, the neuronal classification of the ivory shell Spotted Babylon, Babylonia areolata, was readily demonstrated. Regarding its importance as marine economic molluscan species, the attempt to understand the neuroendocrine regulation is necessary. This study firstly demonstrated the neurosecretory cells as well as the existence and distribution of the egg-laying hormone (ELH)-like peptide in the central nervous system (CNS) and ovary of the B. areolata. The neurosecretory cell was characterized by the cytoplasmic purple dot-like structure as stained by the Gomori's paraldehyde fuchsin...
September 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28917710/molecular-identification-of-vermamoeba-vermiformis-from-freshwater-fish-in-lake-taal-philippines
#18
Giovanni D Milanez, Frederick R Masangkay, Rey C Thomas, Ma Olive Grace O Ordona, Gabriel Q Bernales, Vyanna Camille M Corpuz, Hannah Selina V Fortes, Charezze Margarette S Garcia, Larra Camille Nicolas, Veeranoot Nissapatorn
Free Living Amoebae (FLA) are considered ubiquitous. FLAs may infect various biological organisms which act as reservoir hosts. Infected freshwater fishes can pose a public health concern due to possible human consumption. This study aims to identify possible pathogenic FLAs present in freshwater fishes. Seventy five (75) Oreochromis niloticus were studied for the presence of FLAs. Fish organs were suspended in physiologic saline pelleted and cultured in non-nutrient agar (NNA) lawned with Escherichia coli and were incubated in 33 °C for 14 days...
September 13, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28917670/morphological-characterization-and-biocontrol-effects-of-vibrio-vulnificus-phages-against-vibriosis-in-the-shrimp-aquaculture-environment
#19
P Srinivasan, P Ramasamy
The re-emerging field of phage therapy is the potential biocontrol agents for the transfer of virulence factor and to kill their bacterial hosts. In this study, the lytic Vibrio vulnificus phages were studied to provide a better understanding of phage-host interactions and development of phage therapy. Four new V. vulnificus phages were detected from shrimp aquaculture system, named VV1, VV2, VV3 and VV4. All lytic V. vulnificus phages are the Tecviruses of the family Tectiviridae with typical double layered elongated icosahedral head and tailless morphology...
September 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28917486/effects-of-the-potential-probiotics-bacillus-aerophilus-kadr3-in-inducing-immunity-and-disease-resistance-in-labeo-rohita
#20
Dharmaraj Ramesh, Sami Souissi, Tharifkhan Shan Ahamed
The aim of the present study was to evaluate the dietary administration of Bacillus aerophilus KADR3 on enhancing the immunity and disease resistance against Aeromonas hydrophila infection in Labeo rohita. B. aerophilus culture supernatant exhibited antagonistic activity against fish pathogenic bacteria in an agar well diffusion assay. Four fish groups were fed with either of following diets containing different concentrations of B. aerophilus KADR3 (cfu g(-1)): 0 (control), 1 × 10(7) (DI), 1 × 10(8) (DII), and 1 × 10(9) (DIII) -cfu g(-1)...
September 14, 2017: Fish & Shellfish Immunology
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