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https://www.readbyqxmd.com/read/28325977/salix-transect-of-europe-structured-genetic-variation-and-isolation-by-distance-in-the-nettle-psyllid-trioza-urticae-psylloidea-hemiptera-from-greece-to-arctic-norway
#1
Rungtip Wonglersak, Quentin Cronk, Diana Percy
BACKGROUND: The common nettle (Urtica dioica L.) is co-associated with willows (Salix spp.) in riparian habitats across Europe. We sampled the widespread nettle psyllid, Trioza urticae (Linné, 1758), from Urtica in willow habitats on a megatransect of Europe from the Aegean to the Arctic Ocean. The aim of this study was to use an unusually widespread insect to assess the influence of geographic distances and natural geographic barriers on patterns of genetic variation and haplotype distribution...
2017: Biodiversity Data Journal
https://www.readbyqxmd.com/read/28306507/structural-and-molecular-mechanisms-of-cytokine-mediated-endocrine-resistance-in-human-breast-cancer-cells
#2
Joshua D Stender, Jerome C Nwachukwu, Irida Kastrati, Yohan Kim, Tobias Strid, Maayan Yakir, Sathish Srinivasan, Jason Nowak, Tina Izard, Erumbi S Rangarajan, Kathryn E Carlson, John A Katzenellenbogen, Xin-Qiu Yao, Barry J Grant, Hon S Leong, Chin-Yo Lin, Jonna Frasor, Kendall W Nettles, Christopher K Glass
Human breast cancers that exhibit high proportions of immune cells and elevated levels of pro-inflammatory cytokines predict poor prognosis. Here, we demonstrate that treatment of human MCF-7 breast cancer cells with pro-inflammatory cytokines results in ERα-dependent activation of gene expression and proliferation, in the absence of ligand or presence of 4OH-tamoxifen (TOT). Cytokine activation of ERα and endocrine resistance is dependent on phosphorylation of ERα at S305 in the hinge domain. Phosphorylation of S305 by IKKβ establishes an ERα cistrome that substantially overlaps with the estradiol (E2)-dependent ERα cistrome...
March 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28249564/combining-phylogenetic-footprinting-with-motif-models-incorporating-intra-motif-dependencies
#3
Martin Nettling, Hendrik Treutler, Jesus Cerquides, Ivo Grosse
BACKGROUND: Transcriptional gene regulation is a fundamental process in nature, and the experimental and computational investigation of DNA binding motifs and their binding sites is a prerequisite for elucidating this process. Approaches for de-novo motif discovery can be subdivided in phylogenetic footprinting that takes into account phylogenetic dependencies in aligned sequences of more than one species and non-phylogenetic approaches based on sequences from only one species that typically take into account intra-motif dependencies...
March 1, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28245277/environmental-dna-reflects-spatial-and-temporal-jellyfish-distribution
#4
Toshifumi Minamoto, Miho Fukuda, Koki R Katsuhara, Ayaka Fujiwara, Shunsuke Hidaka, Satoshi Yamamoto, Kohji Takahashi, Reiji Masuda
Recent development of environmental DNA (eDNA) analysis allows us to survey underwater macro-organisms easily and cost effectively; however, there have been no reports on eDNA detection or quantification for jellyfish. Here we present the first report on an eDNA analysis of marine jellyfish using Japanese sea nettle (Chrysaora pacifica) as a model species by combining a tank experiment with spatial and temporal distribution surveys. We performed a tank experiment monitoring eDNA concentrations over a range of time intervals after the introduction of jellyfish, and quantified the eDNA concentrations by quantitative real-time PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28221885/antibacterial-and-antiadhesive-activities-of-extracts-from-edible-plants-against-soft-drink-spoilage-by-asaia-spp
#5
Hubert Antolak, Agata Czyzowska, Dorota Kregiel
This study was conducted to investigate the antibacterial and antiadhesive activities of ethanol extracts from five edible plant parts: cinnamon bark ( Cinnamomum zeylanicum ), licorice root ( Glycyrrhiza radix ), nettle leaves ( Urtica dioica ), green tea leaves ( Camellia sinensis ), and elderberry flowers ( Sambucus nigra ). The chemical constituents of the extracts were identified using high-performance liquid chromatography and liquid chromatography plus mass spectrometry. Six strains of Asaia lannensis and Asaia bogorensis bacteria isolated from spoiled commercial fruit-flavored noncarbonated mineral water were used...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28188411/first-record-and-description-of-juvenile-stages-of-longidorus-artemisiae-rubtsova-chizhov-subbotin-1999-nematoda-longidoridae-in-poland-and-new-data-on-l-juglandicola-li%C3%A5-kov%C3%A3-robbins-brown-1997-based-on-topotype-specimens-from-slovakia
#6
Franciszek Wojciech Kornobis, Marek Renčo, Anna Filipiak
This paper presents the first geographical record of the needle nematode Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 outside Russia. This species was found in Poland near the city of Skierniewice in association with nettle (Urtica dioica L.). Morphometric and morphological data are provided, including the first description of juveniles of this species. Nematodes of the Polish population differ from the type-population in Russia in possessing a thicker body (lower 'a' index) in both sexes; males having a longer body and longer spicules; different sex ratio (1:2 in Polish population vs 1:1 in the type-population) and somewhat less expanded lips...
March 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28160759/five-herbs-plus-thiamine-reduce-pain-and-improve-functional-mobility-in-patients-with-pain-a-pilot-study
#7
Robert Hedaya
Context • Five herbs-Urtica dioica (stinging nettle), Boswellia serrata, Equisetum arvense, Allium sativum, and Apium graveolens-have been demonstrated to have activity at several anti-inflammatory pathways and have analgesic properties that are effective in treating chronic musculoskeletal pain. Objectives • The study intended to evaluate the clinical efficacy of a proprietary blend of U dioica, B serrata, E arvense, A sativum, A graveolens, and thiamine (vitamin B1), or "the blend," in the treatment of chronic musculoskeletal pain...
January 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28158779/maintaining-the-feelings-of-others-in-working-memory-is-associated-with-activation-of-the-left-anterior-insula-and-left-frontal-parietal-control-network
#8
Ryan Smith, Richard D Lane, Anna Alkozei, Jennifer Bao, Courtney Smith, Anna Sanova, Matthew Nettles, William D S Killgore
No abstract text is available yet for this article.
February 1, 2017: Social Cognitive and Affective Neuroscience
https://www.readbyqxmd.com/read/28142266/inflammatory-microenvironment-persists-after-bone-healing-in-intra-articular-ankle-fractures
#9
Samuel B Adams, Elizabeth M Leimer, Lori A Setton, Richard D Bell, Mark E Easley, Janet L Huebner, Thomas V Stabler, Virginia B Kraus, Steven A Olson, Dana L Nettles
BACKGROUND: Post-traumatic osteoarthritis (PTOA) is responsible for the majority of cases of ankle arthritis. While acute and end-stage intra-articular inflammation has previously been described, the state of the joint between fracture healing and end-stage PTOA remains undefined. This study characterized synovial fluid (SF) composition of ankles after bone healing of an intra-articular fracture to identify factors that may contribute to the development of PTOA. METHODS: Of an original 21 patients whose SF was characterized acutely following intra-articular ankle fractures, 7 returned for planned hardware (syndesmotic screw) removal after bone healing (approximately 6 months) and consented to a second bilateral SF collection...
January 1, 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/28130227/unrealistic-phylogenetic-trees-may-improve-phylogenetic-footprinting
#10
Martin Nettling, Hendrik Treutler, Jesus Cerquides, Ivo Grosse
MOTIVATION: The computational investigation of DNA binding motifs from binding sites is one of the classic tasks in bioinformatics and a prerequisite for understanding gene regulation as a whole. Due to the development of sequencing technologies and the increasing number of available genomes, approaches based on phylogenetic footprinting become increasingly attractive. Phylogenetic footprinting requires phylogenetic trees with attached substitution probabilities for quantifying the evolution of binding sites, but these trees and substitution probabilities are typically not known and cannot be estimated easily...
January 27, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28094324/early-life-adversity-accelerates-cellular-ageing-and-affects-adult-inflammation-experimental-evidence-from-the-european-starling
#11
Daniel Nettle, Clare Andrews, Sophie Reichert, Tom Bedford, Claire Kolenda, Craig Parker, Carmen Martin-Ruiz, Pat Monaghan, Melissa Bateson
Early-life adversity is associated with accelerated cellular ageing during development and increased inflammation during adulthood. However, human studies can only establish correlation, not causation, and existing experimental animal approaches alter multiple components of early-life adversity simultaneously. We developed a novel hand-rearing paradigm in European starling nestlings (Sturnus vulgaris), in which we separately manipulated nutritional shortfall and begging effort for a period of 10 days. The experimental treatments accelerated erythrocyte telomere attrition and increased DNA damage measured in the juvenile period...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28087322/cutaneous-exposure-to-vesicant-phosgene-oxime-acute-effects-on-the-skin-and-systemic-toxicity
#12
Neera Tewari-Singh, Dinesh G Goswami, Rama Kant, Claire R Croutch, Robert P Casillas, David J Orlicky, Rajesh Agarwal
Phosgene Oxime (CX), an urticant or nettle agent categorized as a vesicant, is a potential chemical warfare and terrorist weapon. Its exposure can result in widespread and devastating effects including high mortality due to its fast penetration and ability to cause immediate severe cutaneous injury. It is one of the least studied chemical warfare agents with no effective therapy available. Thus, our goal was to examine the acute effects of CX following its cutaneous exposure in SKH-1 hairless mice to help establish a relevant injury model...
January 11, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28073390/the-behavioural-constellation-of-deprivation-causes-and-consequences
#13
Gillian V Pepper, Daniel Nettle
Socioeconomic differences in behaviour are pervasive and well documented, but their causes are not yet well understood. Here, we make the case that there is a cluster of behaviours associated with lower socioeconomic status, which we call the behavioural constellation of deprivation. We propose that the relatively limited control associated with lower socioeconomic status curtails the extent to which people can expect to realise deferred rewards, leading to more present-oriented behaviour in a range of domains...
January 11, 2017: Behavioral and Brain Sciences
https://www.readbyqxmd.com/read/28072579/development-of-advanced-signal-processing-and-source-imaging-methods-for-superparamagnetic-relaxometry
#14
Ming-Xiong Huang, Bill Anderson, Charles W Huang, Gerd J Kunde, Erika C Vreeland, Jeffrey W Huang, Andrei N Matlashov, Todor Karaulanov, Christopher P Nettles, Andrew Gomez, Kayla Minser, Caroline Weldon, Giulio Paciotti, Michael Harsh, Roland R Lee, Edward R Flynn
Superparamagnetic relaxometry (SPMR) is a highly sensitive technique for the in vivo detection of tumor cells and may improve early stage detection of cancers. SPMR employs superparamagnetic iron oxide nanoparticles (SPION). After a brief magnetizing pulse is used to align the SPION, SPMR measures the time decay of SPION using super-conducting quantum interference device (SQUID) sensors. Substantial research has been carried out in developing the SQUID hardware and in improving the properties of the SPION. However, little research has been done in the pre-processing of sensor signals and post-processing source modeling in SPMR...
February 7, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28070327/nutritional-antioxidative-and-antimicrobial-analysis-of-the-mediterranean-hackberry-celtis-australis-l
#15
Ajda Ota, Ana Miklavčič Višnjevec, Rajko Vidrih, Željko Prgomet, Marijan Nečemer, Janez Hribar, Nina Gunde Cimerman, Sonja Smole Možina, Milena Bučar-Miklavčič, Nataša Poklar Ulrih
Celtis australis is a deciduous tree commonly known as Mediterranean hackberry or the European nettle tree. The fruit of hackberry are seldom used for nutritional purposes. The nutritional and physicochemical properties of ripe hackberry fruit from Istria (Marasi village near Vrsar, Croatia) were determined, including water, total fiber, protein, vitamin, mineral, and phenolic contents. This analysis demonstrates that the hackberry fruit is a valuable source of dietary fiber, protein, and vitamins, and of pigments such as lutein, β-carotene, zeaxanthin, and tocopherols...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28063268/evaluation-of-the-quality-of-herbal-teas-by-dart-tof-ms
#16
Jana Prchalová, František Kovařík, Aleš Rajchl
The paper focuses on the optimization, settings and validation of direct analysis in real time (DART) coupled with time of flight detector (TOF) when used for the evaluation of the quality of selected herbal teas (fennel, chamomile, nettle, linden, peppermint, thyme, lemon balm, marigold, sage, rose hip, St. John's Wort). The ionization mode, the optimal ionization temperature and the type of solvent for sample extraction were optimized. The characteristic compounds of the analysed herbal teas (glycosides, flavonoids, phenolic and terpenic substances, such as chamazulene, anethole, menthol, thymol, salviol, hypericin etc...
January 7, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28046007/substrates-and-inhibitors-of-samhd1
#17
Joseph A Hollenbaugh, Jadd Shelton, Sijia Tao, Sheida Amiralaei, Peng Liu, Xiao Lu, Russell W Goetze, Longhu Zhou, James H Nettles, Raymond F Schinazi, Baek Kim
SAMHD1 hydrolyzes 2'-deoxynucleoside-5'-triphosphates (dNTPs) into 2'-deoxynucleosides and inorganic triphosphate products. In this paper, we evaluated the impact of 2' sugar moiety substitution for different nucleotides on being substrates for SAMHD1 and mechanisms of actions for the results. We found that dNTPs ((2'R)-2'-H) are only permissive in the catalytic site of SAMHD1 due to L150 exclusion of (2'R)-2'-F and (2'R)-2'-OH nucleotides. However, arabinose ((2'S)-2'-OH) nucleoside-5'-triphosphates analogs are permissive to bind in the catalytic site and be hydrolyzed by SAMHD1...
2017: PloS One
https://www.readbyqxmd.com/read/28042045/systems-structural-biology-analysis-of-ligand-effects-on-er%C3%AE-predicts-cellular-response-to-environmental-estrogens-and-anti-hormone-therapies
#18
Jerome C Nwachukwu, Sathish Srinivasan, Nelson E Bruno, Jason Nowak, Nicholas J Wright, Filippo Minutolo, Erumbi S Rangarajan, Tina Izard, Xin-Qui Yao, Barry J Grant, Douglas J Kojetin, Olivier Elemento, John A Katzenellenbogen, Kendall W Nettles
Environmental estrogens and anti-hormone therapies for breast cancer have diverse tissue- and signaling-pathway-selective outcomes, but how estrogen receptor alpha (ERα) mediates this phenotypic diversity is poorly understood. We implemented a statistical approach to allow unbiased, parallel analyses of multiple crystal structures, and identified subtle perturbations of ERα structure by different synthetic and environmental estrogens. Many of these perturbations were in the sub-Å range, within the noise of the individual structures, but contributed significantly to the activities of synthetic and environmental estrogens...
January 19, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28032978/exploring-the-structural-compliancy-versus-specificity-of-the-estrogen-receptor-using-isomeric-three-dimensional-ligands
#19
Naina Sharma, Kathryn E Carlson, Jerome C Nwachukwu, Sathish Srinivasan, Abhishek Sharma, Kendall W Nettles, John A Katzenellenbogen
The estrogen receptors (ERs) bind with high affinity to many structurally diverse ligands by significantly distorting the contours of their ligand-binding pockets. This raises a question: To what degree is ER able to distinguish between structurally related regioisomers and enantiomers? We have explored the structural compliance and specificity of ERα with a set of ligands having a 7-oxa-bicyclo[2.2.1]hept-5-ene sulfonate core and basic side chains typical of selective ER modulators (SERMs). These ligands have two regioisomers, each of which is a racemate of enantiomers...
February 17, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28025297/temperature-dependence-of-ice-on-rock-friction-at-realistic-glacier-conditions
#20
C McCarthy, H Savage, M Nettles
Using a new biaxial friction apparatus, we conducted experiments of ice-on-rock friction in order to better understand basal sliding of glaciers and ice streams. A series of velocity-stepping and slide-hold-slide tests were conducted to measure friction and healing at temperatures between -20°C and melting. Experimental conditions in this study are comparable to subglacial temperatures, sliding rates and effective pressures of Antarctic ice streams and other glaciers, with load-point velocities ranging from 0...
February 13, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
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