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https://www.readbyqxmd.com/read/28812241/plasma-glycoproteomic-study-of-therapeutic-hypothermia-reveals-novel-markers-predicting-neurologic-outcome-post-cardiac-arrest
#1
Wenjun Deng, Jing Cao, Lei Chen, David McMullin, James L Januzzi, Ferdinando S Buonanno, Eng H Lo, MingMing Ning
Therapeutic hypothermia (TH) is a neuroprotective treatment post-cardiac arrest but is grossly underutilized. After TH induction, traditional biomarkers and parameters can no long predict clinical outcome due to a lack of understanding of hypothermic response. Innovative approaches to better understand the clinical effect of TH will help to prognosticate outcome and expand beneficial population. Protein glycosylation is an important extracellular post-translational modification, regulating various extracellular signaling pathways...
August 15, 2017: Translational Stroke Research
https://www.readbyqxmd.com/read/28811027/design-of-carbohydrate-electron-transfer-peptides-for-human-histocytic-lymphoma-cell-sensing
#2
Kazuharu Sugawara, Toshihiko Kadoya, Hideki Kuramitz, Yoshihiro Mihara
A carbohydrate/electro-transfer peptide probe was fabricated to perform cell sensing. The probe consisted of a cello-oligosaccharide that was created by the conjugation of an electron-transfer peptide (Y5C) and a carbohydrate via a Schiff base. An oxidation wave due to a phenolic hydroxyl group was obtained by scanning with a glassy carbon electrode. This cell-sensing system was based on a competitive reaction between carbohydrates on a cell surface and the probe as each reacted to a protein that recognized the carbohydrate...
August 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28803003/pathogenic-features-and-characteristics-of-food-borne-pathogens-biofilm-biomass-viability-and-matrix
#3
REVIEW
Shiqi Lin, Ling Yang, Chen Gu, Bing Li, Dingqiang Chen, Lin Li, Zhenbo Xu
Biofilm is a ubiquitous growth pattern of bacterial species survival but is notorious for its threat on public health and food contamination. Extensive studies of the biofilm structure, formation, quantification, quorum sensing system and underlying control strategies have been reported during the past decades. Insightful elucidation of the pathogenic features and characteristic of bacterial biofilm can facilitate in devising appropriate control strategies for biofilm eradication. Therefore, this review mainly summarized the pathogenic features of biofilms from food borne microorganisms, including the biomass (which could be quantified using crystal violet and fluorogenic dye Syto9 assays), viability (which could be determined by tetrazolium salts, fluorescein diacetate, resazurin staining and alamar blue assays) and matrix (which are commonly detected by dimethyl methylene blue and wheat germ agglutinin assays)...
August 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28782554/endocytosis-in-primary-mesenchyme-cells-during-sea-urchin-larval-skeletogenesis
#4
Christopher E Killian, Fred H Wilt
The sea urchin larval embryo elaborates two calcitic endoskeletal elements called spicules. Spicules are synthesized by the primary mesenchyme cells (PMCs) and begin to form at early gastrula stage. It is known that the calcium comprising the spicules comes from the seawater and we wish to further consider the mode of calcium transport from the extracellular seawater to the PMCs and then onto the forming spicules. We used PMC in vitro cultures, calcein, fluorescently labeled dextran, and fluorescently labeled Wheat Germ Agglutinin (WGA) to track calcium transport from the seawater into PMCs and spicules and to determine how molecules from the surface of PMCs interact with the incoming calcium...
August 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28755365/wheat-germ-cell-free-overexpression-for-the-production-of-membrane-proteins
#5
Marie-Laure Fogeron, Aurélie Badillo, François Penin, Anja Böckmann
Due to their hydrophobic nature, membrane proteins are notoriously difficult to express in classical cell-based protein expression systems. Often toxic, they also undergo degradation in cells or aggregate in inclusion bodies, making delicate issues further solubilization and renaturation. These are major bottlenecks in their structural and functional analysis. The wheat germ cell-free (WGE-CF) system offers an effective alternative not only to classical cell-based protein expression systems but also to other cell-free systems for the expression of membrane proteins...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28752591/comparison-and-evaluation-of-capacitation-and-acrosomal-reaction-in-freeze-thawed-human-ejaculated-spermatozoa-treated-with-l-carnitine-and-pentoxifylline
#6
E Aliabadi, S Jahanshahi, T Talaei-Khozani, M Banaei
Cryopreservation is used to preserve the spermatozoa; however, it leads to a reduction in sperm quality. L-carnitine (LC) influences sperm motility and preserves the sperm membrane and DNA integrity. The objectives of this study were to evaluate the protective effects of LC on the membrane integrity of normal human spermatozoa and compare it with pentoxifylline (PT) during cryopreservation. Thirty normal semen samples, prepared by swim-up procedure, were divided into three aliquots: a control without any treatment and two experimental aliquots that were incubated in PT or LC for 30 min...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28727810/cucumber-mosaic-virus-coat-protein-modulates-the-accumulation-of-2b-protein-and-antiviral-silencing-that-causes-symptom-recovery-in-planta
#7
Xiao-Peng Zhang, De-Shui Liu, Teng Yan, Xiao-Dong Fang, Kai Dong, Jin Xu, Ying Wang, Jia-Lin Yu, Xian-Bing Wang
Shoot apical meristems (SAM) are resistant to most plant viruses due to RNA silencing, which is restrained by viral suppressors of RNA silencing (VSRs) to facilitate transient viral invasion of the SAM. In many cases chronic symptoms and long-term virus recovery occur, but the underlying mechanisms are poorly understood. Here, we found that wild-type Cucumber mosaic virus (CMVWT) invaded the SAM transiently, but was subsequently eliminated from the meristems. Unexpectedly, a CMV mutant, designated CMVRA that harbors an alanine substitution in the N-terminal arginine-rich region of the coat protein (CP) persistently invaded the SAM and resulted in visible reductions in apical dominance...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28705398/very-long-chain-aldehydes-induce-appressorium-formation-in-ascospores-of-the-wheat-powdery-mildew-fungus-blumeria-graminis
#8
Mo Zhu, Markus Riederer, Ulrich Hildebrandt
Asexually produced conidia of the wheat powdery mildew fungus Blumeria graminis f. sp. tritici (Bgt) are known to perceive cuticular very-long-chain aldehydes as signal substances strongly stimulating germination and differentiation of infection structures in a concentration- and chain-length-dependent manner. Conidial germination and appressorium formation are widely prevented by the presence of free water on the host surface. However, sexually produced ascospores can differentiate immersed in water. Applying a Formvar(®)-based in vitro-system showed that ascospore appressorium formation was strongly induced by the presence of wheat leaf cuticular wax...
August 2017: Fungal Biology
https://www.readbyqxmd.com/read/28698513/anti-inflammatory-activity-of-citric-acid-treated-wheat-germ-extract-in-lipopolysaccharide-stimulated-macrophages
#9
Hee-Yeong Jeong, Yong-Seok Choi, Jae-Kang Lee, Beom-Joon Lee, Woo-Ki Kim, Hee Kang
Until recently, fermentation was the only processing used to improve the functionality of wheat germ. The release of 2,6-dimethoxy-1,4-benzoquinone (DMBQ) from hydroquinone glycosides during the fermentation process is considered a marker of quality control. Here, we treated wheat germ extract with citric acid (CWG) to release DMBQ and examined the anti-inflammatory activity of this extract using a lipopolysaccharide-activated macrophage model. Treatment of wheat germ with citric acid resulted in detectable release of DMBQ but reduced total phenolic and total flavonoid contents compared with untreated wheat germ extract (UWG)...
July 10, 2017: Nutrients
https://www.readbyqxmd.com/read/28693732/wheat-germ-agglutinin-and-lens-culinaris-agglutinin-sensitized-anisotropic-silver-nanoparticles-in-detection-of-bacteria-a-simple-photometric-assay
#10
Mariam V Mikaelyan, Gayane G Poghosyan, Olga D Hendrickson, Boris B Dzantiev, Vardan K Gasparyan
Efficacy of anisotropic silver nanoparticles sensitized with wheat germ agglutinin (WGA) and Lens culinaris agglutinin (LCA) was studied for detection of Staphylococcus aureus and Escherichia coli. It was demonstrated that interaction of these nanoparticles with bacteria stabilizes them and prevents their aggregation upon addition of sodium chloride; such stabilization depends on bacteria concentration. High concentration of bacteria results in higher stabilization whereas low concentration leads to aggregation of nanoparticles...
August 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28688084/chemical-proteomic-analysis-of-6-benzylaminopurine-molecular-partners-in-wheat-grains
#11
Radim Simerský, Ivo Chamrád, Jindřich Kania, Miroslav Strnad, Marek Šebela, René Lenobel
An affinity-based chemical proteomic technique enabled direct identification of BAP-interacting proteins in wheat, including the well-known cytokinin-binder, cytokinin-binding protein 1. In this work, we show the development of a chemical proteomic technique for the identification of proteins binding to natural aromatic cytokinins (CKs). 6-benzylaminopurine (BAP) and documented CK-binder, wheat germ-allocated cytokinin-binding protein 1 (CBP-1), were suggested as an ideal proof-of concept affinity pair. Therefore, wheat grains were chosen as a model plant material...
July 7, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28686627/wga-based-lectin-affinity-gel-electrophoresis-a-novel-method-for-the-detection-of-o-glcnac-modified-proteins
#12
Yuji Kubota, Ko Fujioka, Mutsuhiro Takekawa
Post-translational modification with O-linked β-N-acetylglucosamine (O-GlcNAc) occurs selectively on serine and/or threonine residues of cytoplasmic and nuclear proteins, and dynamically regulates their molecular functions. Since conventional strategies to evaluate the O-GlcNAcylation level of a specific protein require time-consuming steps, the development of a rapid and easy method for the detection and quantification of an O-GlcNAcylated protein has been a challenging issue. Here, we describe a novel method in which O-GlcNAcylated and non-O-GlcNAcylated forms of proteins are separated by lectin affinity gel electrophoresis using wheat germ agglutinin (WGA), which primarily binds to N-acetylglucosamine residues...
2017: PloS One
https://www.readbyqxmd.com/read/28667579/the-impact-of-fish-oil-and-wheat-germ-oil-combination-on-mineral-bone-and-inflammatory-markers-in-maintenance-hemodialysis-patients-a-randomized-double-blind-placebo-controlled-clinical-trial
#13
Hadeer Zakaria, Tarek M Mostafa, Gamal A El-Azab, Ahmed M Abd El Wahab, Heba Elshahawy, Nagy Ah Sayed-Ahmed
PURPOSE: This study aimed to examine the impact of combined supplementation of fish oil (FO) with antioxidants like wheat germ oil (WGO) on mineral-bone and inflammatory markers in maintenance HD patients. METHODS: This randomized, double-blind, placebo-controlled trial involved 46 HD patients who were randomly assigned into two groups to receive daily 3000 mg of FO [1053 mg omega-3 fatty acids (ω-3 FAs)] plus 300 mg of WGO [0.765 mg vitamin E] or placebo for 4 months...
June 30, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28652201/incorporation-of-in-vitro-digestive-enzymes-in-an-intestinal-epithelial-cell-line-model-for-protein-hazard-identification
#14
Lauren K Markell, Stephanie M Wezalis, Jason M Roper, Cindi Zimmermann, Bryan Delaney
Relatively few proteins in nature produce adverse effects following oral exposure. Of those that do, effects are often observed in the gut, particularly on intestinal epithelial cells (IEC). Previous studies reported that addition of protein toxins to IEC lines disrupted monolayer integrity but innocuous dietary proteins did not. Studies presented here investigated the effects of innocuous (bovine serum albumin, β-lactoglobulin, RuBisCO, fibronectin) or hazardous (phytohaemagglutinin-E, concanavalin A, wheat germ agglutinin, melittin) proteins that either were untreated or exposed to digestive enzymes prior to addition to Caco-2 human IEC line monolayers...
June 23, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28648723/a-protocol-for-the-simultaneous-identification-of-chitin-containing-particles-and-their-associated-bacteria
#15
Florencia Biancalana, Germán A Kopprio, Rubén J Lara, Cecilia Alonso
Chitin is the second most abundant polymer on Earth, playing a crucial role in the biogeochemical cycles. A core issue for studying its processing in aquatic systems is the identification and enumeration of chitin-containing particles and organisms, ideally in a manner that can be directly linked to bulk chitin quantification. The aim of this study was the development of such a technique. We successfully combined the methodology of bulk chitin determination using wheat germ agglutinin (FITC-WGA) for staining chitin-containing particles and organisms along with CARD-FISH staining of either chitin-containing eukaryotic cells or bacteria associated with them...
July 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28639357/cerebellar-projections-to-the-ventral-lateral-geniculate-nucleus-and-the-thalamic-reticular-nucleus-in-the-cat
#16
Hiroyuki Nakamura
The ventral lateral geniculate nucleus (LGNv) is a retinorecipient part of the ventral thalamus and in cats, it consists of medial (M), medial intermediate (IM), lateral intermediate (IL), lateral (L), and dorsal (D) subdivisions. These subdivisions can be differentiated not only by their cytoarchitecture, but also by their connectivity and putative functions. The LGNv may play a role in visuomotor gating, in that there is evidence of cerebellar afferent projections to the intermediate subdivisions. The cerebellar posterior interpositus (IP) and lateral (LC) nuclei are known to project to IM and IL, but the specifics of these projections are unclear...
June 22, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28635255/characterization-and-immunomodulatory-activity-of-a-novel-peptide-ecfsta-from-wheat-germ-globulin
#17
Wenjia Wu, Mengmeng Zhang, Yao Ren, Xinze Cai, Zhina Yin, Xiaoai Zhang, Tian Min, Hui Wu
A novel peptide was extracted from wheat germ globulin and purified using ion-exchange chromatography, gel filtration chromatography, and semi-preparative reversed-phase high-performance liquid chromatography (RP-HPLC). The sequence of the peptide was found to be Glu-Cys-Phe-Ser-Thr-Ala (ECFSTA). Its immunomodulatory effects were evaluated, and the results showed that ECFSTA could enhance phagocytosis of RAW 264.7 cells and significantly increase their secretion of nitric oxide (NO), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and reactive oxygen species (ROS)...
July 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28633842/effects-of-multi-frequency-ultrasound-pretreatment-under-low-power-density-on-the-enzymolysis-and-the-structure-characterization-of-defatted-wheat-germ-protein
#18
Xue Yang, Yunliang Li, Suyun Li, Ayobami Olayemi Oladejo, Yucheng Wang, Shanfen Huang, Cunshan Zhou, Yang Wang, Li Mao, Yanyan Zhang, Haile Ma, Xiaofei Ye
The effects of ultrasonic frequency mode, power density, pretreatment time and other parameters under low power density on the degree of hydrolysis (DH) of defatted wheat germ protein (DWGP) and angiotensin-I-converting enzyme (ACE) inhibitory activity of DWGP hydrolysate were studied in this research. Ultraviolet-visible (UV-Vis) spectra, free sulfhydryl (SH), disulfide bond (SS), surface hydrophobicity and hydrophobic protein content of ultrasound-pretreated protein and hydrophobic amino acid (HAA) content of alcalase-hydrolysate of DWGP were measured under optimized ultrasonic condition...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28626561/enantioselective-carbohydrate-recognition-by-synthetic-lectins-in-water
#19
Pablo Ríos, Tiddo J Mooibroek, Tom S Carter, Christopher Williams, Miriam R Wilson, Matthew P Crump, Anthony P Davis
Carbohydrate receptors with a chiral framework have been generated by combining a tetra-aminopyrene and a C3-symmetrical triamine via isophthalamide spacers bearing water-solubilising groups. These "synthetic lectins" are the first to show enantiodiscrimination in aqueous solution, binding N-acetylglucosamine (GlcNAc) with 16 : 1 enantioselectivity. They also show exceptional affinities. GlcNAc is bound with Ka up to 1280 M(-1), more than twice that measured for previous synthetic lectins, and three times the value for wheat germ agglutinin, the lectin traditionally employed to bind GlcNAc in glycobiological research...
May 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28622359/aerobic-exercise-protects-against-pressure-overload-induced-cardiac-dysfunction-and-hypertrophy-via-%C3%AE-3-ar-nnos-no-activation
#20
Bin Wang, Ming Xu, Wenju Li, Xiaoli Li, Qiangsun Zheng, Xiaolin Niu
Aerobic exercise confers sustainable protection against cardiac hypertrophy and heart failure (HF). Nitric oxide synthase (NOS) and nitric oxide (NO) are known to play an important role in exercise-mediated cardioprotection, but the mechanism of NOS/NO stimulation during exercise remains unclear. The aim of this study is to determine the role of β3-adrenergic receptors (β3-ARs), NOS activation, and NO metabolites (nitrite and nitrosothiols) in the sustained cardioprotective effects of aerobic exercise. An HF model was constructed by transverse aortic constriction (TAC)...
2017: PloS One
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