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https://www.readbyqxmd.com/read/28422419/carbohydrate-analog-microarrays-for-identification-of-lectin-selective-ligands
#1
Injae Shin, Ji Young Hyun, Cheol Wan Park, Yanna Liu, Daeun Kwon, Seong-Hyun Park, Sungjin Park, Jaeyoung Pai
Fifty five mono- and disaccharide analogs were prepared and used for construction of microarrays to uncover lectin-selective ligands. The microarray study shows that two disaccharide analogs, 28' and 44', selectively bind to S. tuberosum lectin (STL) and wheat germ agglutinin (WGA), respectively. The results of cell studies also indicate that 28' and 44' selectively block binding of STL and WGA to mammalian cells unlike a natural ligand LacNAc that suppresses binding of both STL and WGA to cells.
April 19, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28417422/selection-of-an-artificial-diet-for-laboratory-rearing-of-opogona-sacchari-lepidoptera-tineidae-bojer-1856
#2
A Coelho, J M Milanez, R de Andrade Moral, C G B Demétrio, J R P Parra
The banana moth Opogona sacchari (Bojer) (Lepidoptera: Tineidae) is a polyphagous pest that can cause serious damage, in particular to banana crops in southern Brazil. The insect is a quarantine pest in several countries, including Argentina, the main consumer market for bananas from southern Brazil. Little information is available about the biology and ecology of this moth, such as a suitable diet for laboratory rearing. In order to provide support for integrated pest management of the pest, this study furnished data for selecting two diets suitable for continuous laboratory rearing of O...
April 17, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28398642/wga-alexa-conjugates-for-axonal-tracing
#3
Sabrina L Levy, Joshua J White, Elizabeth P Lackey, Lindsey Schwartz, Roy V Sillitoe
Anatomical labeling approaches are essential for understanding brain organization. Among these approaches are various methods of performing tract tracing. However, a major hurdle to overcome when marking neurons in vivo is visibility. Poor visibility makes it challenging to image a desired neuronal pathway so that it can be easily differentiated from a closely neighboring pathway. As a result, it becomes impossible to analyze individual projections or their connections. The tracer that is chosen for a given purpose has a major influence on the quality of the tracing...
April 10, 2017: Current Protocols in Neuroscience
https://www.readbyqxmd.com/read/28391698/pegylated-nanoemulsions-for-oral-delivery-role-of-the-inner-core-on-the-final-fate-of-the-formulation
#4
Manuel J Santander-Ortega, Maria Plaza-Oliver, Virginia Rodriguez-Robledo, Lucia Castro-Vazquez, Noemi Villaseca-Gonzalez, Joaquin Gonzalez-Fuentes, Emilio L Cano, Pilar Marcos, Maria Victoria Lozano, Maria Mar Arroyo-Jimenez
During the last decade, there has been growing interest to grant nanoparticles with diffusion properties across mucosae. In this sense, the non-ionic block copolymer Pluronic F127 (PF127) has arisen as a promising coating agent to formulate mucus penetrating particles (MPP). In the journey to find efficient coating agents, most of the studies have been focused more on the effect of the coating agent architecture rather than on the role the physicochemical properties of the nanoparticle used as substrate...
April 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28380544/digestion-and-nitrogen-balance-using-swine-diets-containing-increasing-proportions-of-coproduct-ingredients-and-formulated-using-the-net-energy-system
#5
J A Acosta, R D Boyd, J F Patience
Rising feed expenditures demand that our industry pursues strategies to lower the cost of production. One option is the adoption of the NE system, although many producers are hesitant to proceed without proof that NE estimates are reliable. The objective of this experiment was to compare the apparent total tract digestibility (ATTD) of energy and nutrients and the N retention (NR) of diets formulated using the NE system with increasing quantities of coproduct ingredients. The 5 dietary treatments included a control corn-soybean meal diet (CTL); the CTL plus 6% each of corn distiller's dried grains with solubles (DDGS), corn germ meal, and wheat middlings and NE equal to the CTL by adding soybean oil (CONS-18); the CONS-18 diet, without oil added, with NE content lower than the CTL (DECL-18); the CTL plus 12% each of corn DDGS, corn germ meal, and wheat middlings and NE equal to the CTL by adding soybean oil (CONS-36); and the CONS-36 diet, without oil added, with NE content lower than the CTL (DECL-36)...
March 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28378857/immunoscreening-of-plasmodium-falciparum-proteins-expressed-in-a-wheat-germ-cell-free-system-reveals-a-novel-malaria-vaccine-candidate
#6
Masayuki Morita, Eizo Takashima, Daisuke Ito, Kazutoyo Miura, Amporn Thongkukiatkul, Ababacar Diouf, Rick M Fairhurst, Mahamadou Diakite, Carole A Long, Motomi Torii, Takafumi Tsuboi
The number of malaria vaccine candidates in preclinical and clinical development is limited. To identify novel blood-stage malaria vaccine candidates, we constructed a library of 1,827P. falciparum proteins prepared using the wheat germ cell-free system (WGCFS). Also, a high-throughput AlphaScreen procedure was developed to measure antibody reactivity to the recombinant products. Purified IgGs from residents in malaria endemic areas have shown functional activity against blood-stage parasites as judged by an in vitro parasite Growth Inhibition Assay (GIA)...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28365703/in-vivo-imaging-of-the-cerebral-endothelial-glycocalyx-in-mice
#7
Jin-Hui Yoon, Eek-Sung Lee, Yong Jeong
BACKGROUND/AIMS: Endothelial glycocalyx refers to the proteoglycan or glycoprotein layer of vessel walls and has critical physiological functions. Cerebral glycocalyx may have additional functions considering the blood-brain barrier and other features. However, the assessment of it has only been performed ex vivo, which includes processes presumably damaging the glycocalyx layer. Here we visualize and characterize the cerebral endothelial glycocalyx in vivo. METHODS: We visualized and quantified the cerebral endothelial glycocalyx in vivo under a 2-photon microscope by tagging glycocalyx and vessel lumen with wheat germ agglutinin lectin and dextran, respectively...
April 1, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/28365442/loss-of-cln3-impacts-protein-secretion-in-the-social-amoeba-dictyostelium
#8
Robert J Huber
Neuronal ceroid lipofuscinosis (NCL), also referred to as Batten disease, is the most common form of childhood neurodegeneration. Mutations in CLN3 cause the most prevalent subtype of the disease, which manifests during early childhood and is currently untreatable. The precise function of the CLN3 protein is still not known, which has inhibited the development of targeted therapies. In the social amoeba Dictyostelium discoideum, loss of the CLN3 homolog, Cln3, reduces adhesion during early development, which delays streaming and aggregation...
March 29, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28359935/a-large-increase-of-sour-taste-receptor-cells-in-skn-1-deficient-mice-does-not-alter-the-number-of-their-sour-taste-signal-transmitting-gustatory-neurons
#9
Naohiro Maeda, Masataka Narukawa, Yoshiro Ishimaru, Kurumi Yamamoto, Takumi Misaka, Keiko Abe
The connections between taste receptor cells (TRCs) and innervating gustatory neurons are formed in a mutually dependent manner during development. To investigate whether a change in the ratio of cell types that compose taste buds influences the number of innervating gustatory neurons, we analyzed the proportion of gustatory neurons that transmit sour taste signals in adult Skn-1a(-/-) mice in which the number of sour TRCs is greatly increased. We generated polycystic kidney disease 1 like 3-wheat germ agglutinin (pkd1l3-WGA)/Skn-1a(+/+) and pkd1l3-WGA/Skn-1a(-/-) mice by crossing Skn-1a(-/-) mice and pkd1l3-WGA transgenic mice, in which neural pathways of sour taste signals can be visualized...
March 28, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28304151/cardiac-cell-proliferation-is-not-necessary-for-exercise-induced-cardiac-growth-but-required-for-its-protection-against-ischaemia-reperfusion-injury
#10
Yihua Bei, Siyi Fu, Xiangming Chen, Mei Chen, Qiulian Zhou, Pujiao Yu, Jianhua Yao, Hongbao Wang, Lin Che, Jiahong Xu, Junjie Xiao
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was utilized to induce cardiac physiological growth. Simultaneously, the antineoplastic agent 5-fluorouracil (5-FU), which acts during the S phase of the cell cycle, was given to mice via intraperitoneal injections. Using EdU and Ki-67 immunolabelling, we showed that exercise-induced cardiac cell proliferation was blunted by 5-FU...
March 17, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28282148/alterations-in-the-salivary-proteome-and-n-glycome-of-sj%C3%A3-gren-s-syndrome-patients
#11
Steven C Hall, Maria E Hassis, Katherine E Williams, Matthew E Albertolle, Akraporn Prakobphol, Andrew B Dykstra, Megan Laurance, Katherine Ona, Richard K Niles, Namrata Prasad, Matthew Gormley, Caroline Shiboski, Lindsey A Criswell, H Ewa Witkowska, Susan J Fisher
We used isobaric mass tagging (iTRAQ) and lectin affinity capture mass spectrometry (MS)-based workflows for global analyses of parotid saliva (PS) and whole saliva (WS) samples obtained from patients diagnosed with primary Sjögren's Syndrome (pSS) who were enrolled in the Sjögren's International Collaborative Clinical Alliance (SICCA) as compared with two control groups. The iTRAQ analyses revealed up- and down-regulation of numerous proteins that could be involved in the disease process (e.g., histones) or attempts to mitigate the ensuing damage (e...
March 24, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28280340/wheat-germ-agglutinin-conjugated-liposomes-incorporated-with-cardiolipin-to-improve-neuronal-survival-in-alzheimer-s-disease-treatment
#12
Yung-Chih Kuo, Che-Yu Lin, Jay-Shake Li, Yung-I Lou
Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer's disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28274431/artificial-neural-network-genetic-algorithm-to-optimize-wheat-germ-fermentation-condition-application-to-the-production-of-two-anti-tumor-benzoquinones
#13
Zi-Yi Zheng, Xiao-Na Guo, Ke-Xue Zhu, Wei Peng, Hui-Ming Zhou
Methoxy-ρ-benzoquinone (MBQ) and 2, 6-dimethoxy-ρ-benzoquinone (DMBQ) are two potential anticancer compounds in fermented wheat germ. In present study, modeling and optimization of added macronutrients, microelements, vitamins for producing MBQ and DMBQ was investigated using artificial neural network (ANN) combined with genetic algorithm (GA). A configuration of 16-11-1 ANN model with Levenberg-Marquardt training algorithm was applied for modeling the complicated nonlinear interactions among 16 nutrients in fermentation process...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28249214/lectin-magnetic-separation-lms-for-isolation-of-toxoplasma-gondii-oocysts-from-concentrated-water-samples-prior-to-detection-by-microscopy-or-qpcr
#14
Jemere Bekele Harito, Andrew T Campbell, Kristoffer Relling Tysnes, J P Dubey, Lucy J Robertson
Although standard methods for analyzing water samples for the protozoan parasites Cryptosporidium spp. and Giardia duodenalis are available and widely used, equivalent methods for analyzing water samples for Toxoplasma gondii oocysts are lacking. This is partly due to the lack of a readily available, reliable immunomagnetic separation technique (IMS). Here we investigated the use of lectin-magnetic separation (LMS) for isolating T. gondii oocysts from water sample concentrates, with subsequent detection by microscopy or molecular methods...
February 21, 2017: Water Research
https://www.readbyqxmd.com/read/28242905/wheat-germ-stabilization-by-infrared-radiation
#15
Renato D Gili, Pablo M Palavecino, M Cecilia Penci, Marcela L Martinez, Pablo D Ribotta
Wheat germ has an important enzymatic activity, being lipases the enzymes which cause the highest impact in the reduction of shelf life. The objective of this study was to evaluate the effects of infrared radiation on wheat germ stabilization in an attempt to extend the shelf life. The effects of treatment time, gap (sample distance to IR emitters) and infrared radiation intensity on wheat germ were analyzed through response surface methodology. Final moisture content, final temperature, color of germ and germ oil quality parameters: free fatty acid content changes and total tocopherol content were the responses evaluated using a Box-Behnken design...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28237713/poly-l-glutamic-acid-drug-delivery-system-for-the-intravesical-therapy-of-bladder-cancer-using-wga-as-targeting-moiety
#16
C Apfelthaler, M Anzengruber, F Gabor, M Wirth
In the management of bladder cancer, surgical resection of the tumour is usually followed by intravesical instillation of immunomodulatives and/or chemotherapeutics. The purpose of this local intravesical therapy is to eliminate residual malignant cells after surgical intervention. The main limitation of a localised adjuvant therapy is the insufficient concentration of the active pharmaceutical ingredient (API) in malignant cells due to the unique structure of the human urothelium making it an exclusively hard to overcome barrier in the human body...
February 22, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28235802/a-cell-free-method-for-expressing-and-reconstituting-membrane-proteins-enables-functional-characterization-of-the-plant-receptor-like-protein-kinase-feronia
#17
Benjamin B Minkoff, Shin-Ichi Makino, Miyoshi Haruta, Emily T Beebe, Russell L Wrobel, Brian G Fox, Michael R Sussman
There are more than 600 receptor-like kinases (RLKs) in Arabidopsis, but due to challenges associated with the characterization of membrane proteins, only a few have known biological functions. The plant RLK FERONIA is a peptide receptor and has been implicated in plant growth regulation, but little is known about its molecular mechanism of action. To investigate the properties of this enzyme, we used a cell-free wheat germ-based expression system in which mRNA encoding FERONIA was co-expressed with mRNA encoding the membrane scaffold protein variant MSP1D1...
April 7, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28218996/hypoxia-stimulated-membrane-trafficking-requires-t-plastin
#18
Marieke Wottawa, Stephanie Naas, Johannes Böttger, Gijsbert J van Belle, Wiebke Möbius, Natalia H Revelo, Doris Heidenreich, Melanie von Ahlen, Anke Zieseniss, Katharina Kröhnert, Susanne Lutz, Christof Lenz, Henning Urlaub, Silvio O Rizzoli, Dörthe M Katschinski
Traffic between the plasma membrane and the endomembrane compartments is an essential feature of eukaryotic cells. The secretory pathway sends cargoes from biosynthetic compartments to the plasma membrane. This is counterbalanced by a retrograde endocytotic route and is essential for cell homeostasis. Cells need to adapt rapidly to environmental challenges like the reduction of pO2 which, however, has not been analyzed in relation to membrane trafficking in detail. Therefore we determined changes in the plasma membrane trafficking in normoxia, hypoxia, and after reoxygenation METHODS: Membrane trafficking was analyzed by using the bulk membrane endocytosis marker FM 1-43, the newly developed membrane probe mCLING, wheat germ agglutinin as well as fluorescently labeled choleratoxin subunit B...
February 20, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28215876/in-vitro-lectin-binding-to-the-outer-surface-of-spirocerca-lupi-at-different-life-stages
#19
I Aroch, I Arogeti, A Marcovics, Y Spiegel, E Lavy
Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28203774/novel-chromatographic-methods-for-simultaneous-quantification-of-fish-and-wheat-germ-oils-mixture-in-pharmaceutical-dosage-forms
#20
Amira F El-Yazbi, Amr El-Hawiet
Two simple, direct and environment-friendly chromatographic methods, high-performance liquid chromatography (HPLC) and high-performance thin layer chromatographic (HPTLC), were developed for the determination of a binary mixture of fish oil (FO) and wheat germ oil (WGO), for the first time, in their pharmaceutical dosage forms with no need for any sample pretreatment. The HPLC separation was carried out using C-18 stationary phase with mobile phase of 15% formic acid (pH 6), methanol and acetonitrile through gradient-elution, 1...
May 1, 2017: Journal of Chromatographic Science
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