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https://www.readbyqxmd.com/read/29467651/functional-g-protein-coupled-receptor-gpcr-synthesis-the-pharmacological-analysis-of-human-histamine-h1-receptor-hrh1-synthesized-by-a-wheat-germ-cell-free-protein-synthesis-system-combined-with-asolectin-glycerosomes
#1
Yasuyuki Suzuki, Tomio Ogasawara, Yuki Tanaka, Hiroyuki Takeda, Tatsuya Sawasaki, Masaki Mogi, Shuang Liu, Kazutaka Maeyama
G-protein-coupled receptors (GPCRs) are membrane proteins distributed on the cell surface, and they may be potential drug targets. However, synthesizing GPCRs in vitro can be challenging. Recently, some cell-free protein synthesis systems have been shown to produce a large amount of membrane protein combined with chemical chaperones that include liposomes and glycerol. Liposomes containing high concentrations of glycerol are known as glycerosomes, which are used in new drug delivery systems. Glycerosomes have greater morphological stability than liposomes...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29463319/performance-of-arma-chinensis-reared-on-an-artificial-diet-formulated-using-transcriptomic-methods
#2
D Y Zou, T A Coudron, L S Zhang, X S Gu, W H Xu, X L Liu, H H Wu
An artificial diet formulated for continuous rearing of the predator Arma chinensis was inferior to natural prey when evaluated using life history parameters. A transcriptome analysis identified differentially expressed genes in diet-fed and prey-fed A. chinensis that were suggestive of molecular mechanisms underlying the nutritive impact of the artificial diet. Changes in the diet formulation were made based on the transcriptome analysis and tested using life history parameters. The quantity of pig liver, chicken egg, tuna fish, biotin, nicotinamide, vitamin B6, thiamine, riboflavin, vitamin C, L-glutamine, and sucrose was reduced, and wheat germ oil, calcium pantothenate and folic acid were increased...
February 21, 2018: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/29453757/localization-of-epithelial-sodium-channel-enac-and-cftr-in-the-germinal-epithelium-of-the-testis-sertoli-cells-and-spermatozoa
#3
Sachin Sharma, Aaron Hanukoglu, Israel Hanukoglu
Spermatogenesis starts within the seminiferous tubules of the testis by mitotic division of spermatogonia that produces spermatocytes. Meiotic division of these spermatocytes produces haploid spermatids that differentiate into spermatozoa. In this study, we examined the expression of ENaC and CFTR (a Cl - channel) in rat testicular sections using confocal microscopic immunofluorescence. The structural integrity of the seminiferous tubule sections was verified by precise phalloidin staining of the actin fibers located abundantly at both basal and adluminal tight junctions...
February 16, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29434991/mechanism-of-the-anti-angiogenic-effect-of-avemar-on-tumor-cells
#4
Nilüfer Gülmen Imir, Esra Aydemir, Ece Şimşek
Avemar, a derivative of fermented wheat germ extract, is a non-toxic and natural compound that is used as a dietary supplement by cancer patients undergoing chemotherapy and radiotherapy. Avemar has numerous biological activities, and several recent studies have reported that it may also have metastatic and anti-angiogenic effects. In the present study, the mechanism of the anti-angiogenic effect of Avemar on human cancer cells was investigated. The human cell lines NCI-N87 (gastric tubular adenocarcinoma), PC3 (prostate carcinoma), HeLa (endocervical adenocarcinoma) and A549 (lung adenocarcinoma) were treated with various doses (400, 800, 1,600 and 3,200 µg/ml) of Avemar, and the changes in mRNA and protein levels of two important markers of angiogenesis, vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (Cox-2), were assessed by reverse transcription-quantitative polymerase chain reaction and ELISA...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29426861/deficiency-of-a-triterpene-pathway-results-in-humidity-sensitive-genic-male-sterility-in-rice
#5
Zheyong Xue, Xia Xu, Yuan Zhou, Xiaoning Wang, Yingchun Zhang, Dan Liu, Binbin Zhao, Lixin Duan, Xiaoquan Qi
In flowering plants, the pollen coat protects the released male germ cells from desiccation and damage during pollination. However, we know little about the mechanism by which the chemical composition of the pollen coat prevents dehydration of pollen grains. Here we report that deficiency of a grass conserved triterpene synthase, OsOSC12/OsPTS1, in rice leads to failure of pollen coat formation. The mutant plants are male sterile at low relative humidity (RH < 60%), but fully male fertile at high relative humidity (>80%)...
February 9, 2018: Nature Communications
https://www.readbyqxmd.com/read/29417655/glycan-distribution-and-density-in-native-skin-s-stratum-corneum
#6
J Danzberger, M Donovan, C Rankl, R Zhu, S Vicic, C Baltenneck, R Enea, P Hinterdorfer, G S Luengo
BACKGROUND: The glycosylation of proteins on the surface of corneocytes is believed to play an important role in cellular adhesion in the stratum corneum (SC) of human skin. Mapping with accuracy the localization of glycans on the surface of corneocytes through traditional methods of immunohistochemistry and electron microscopy remains a challenging task as both approaches lack enough resolution or need to be performed in high vacuum conditions. MATERIALS AND METHODS: We used an advanced mode of atomic force microscope (AFM), with simultaneous topography and recognition imaging to investigate the distribution of glycans on native (no chemical preparation) stripped samples of human SC...
February 7, 2018: Skin Research and Technology
https://www.readbyqxmd.com/read/29385488/analysis-for-low-molecular-weight-carbohydrates-are-needed-to-account-for-all-energy-contributing-nutrients-in-some-feed-ingredients-but-physical-characteristics-do-not-predict-in-vitro-digestibility-of-dry-matter
#7
D M D L Navarro, E M A M Bruininx, L de Jong, H H Stein
An experiment was conducted to quantify nutrient and fiber fractions of feed ingredients and to determine in vitro apparent ileal digestibility (IVAID) and in vitro apparent total tract digestibility (IVATTD) of DM and OM in each ingredient. Ten ingredients that vary in fiber concentration and composition were used: corn, wheat, soybean meal, canola meal, distillers dried grains with solubles (DDGS), corn germ meal, copra expellers, sugar beet pulp (SBP), synthetic cellulose (SF), and pectin. Correlations between chemical and physical characteristics of ingredients and IVAID and IVATTD of DM and OM were determined...
January 29, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29361082/dietary-protein-and-carbohydrate-levels-affect-performance-and-digestive-physiology-of-plodia-interpunctella-lepidoptera-pyralidae
#8
E Borzoui, A R Bandani, S H Goldansaz, R Talaei-Hassanlouei
In this study, life history and nutritional indices of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) was evaluated on six food commodities: dried fig, dried wheat germ, dried white mulberry, groundnut, pistachio, and raisin, compared with artificial diet. The influence of dietary macronutrient content on digestive α-amylase was also assessed. A delay in the developmental time of P. interpunctella immature stages was detected when larvae were fed on raisin. The highest survival rate of immature stages was on the artificial diet, and the lowest was on raisin...
January 18, 2018: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29358834/quality-assessment-of-low-sugar-jams-enriched-with-plant-raw-materials-exhibiting-health-promoting-properties
#9
Anna Banaś, Anna Korus, Małgorzata Tabaszewska
Low-sugar gooseberry jams enriched by the addition of black chokeberry, elderberry, Japanese quince, flax seeds and wheat germs were examined for the content of total polyphenols, total flavonoids, and total anthocyanins as well as their antioxidant activity (DPPH, ABTS, and FRAP) and individual phenolic compounds. The jams were evaluated immediately after production and after 6 and 12 months of storage. Samples were stored at chilled temperature (10 °C) and room temperature (20 °C). A significant increase in the level of the analyzed components and antioxidant activity were determined in jams with the addition of chokeberry, elderberry and Japanese quince, while in the case of other plant ingredients the differences were not always significant...
January 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29357431/vascular-endothelial-cell-changes-in-post-cardiac-surgery-acute-kidney-injury
#10
Saqib H Qureshi, Nishith N Patel, Gavin J Murphy
BACKGROUND: Acute Kidney Injury (AKI) is common complication of cardiac surgery however the phenotype of this condition is poorly defined. The aim of this study was to characterize changes in endothelial structure and function that underlie post cardiopulmonary bypass (CPB) AKI. METHODS: Adult pigs (N=16) were randomised to undergo following procedures; (n=8 per group); Group 1: sham operation-neck dissection with 2.5hr of general anesthesia and Group 2: CPB-2.5hr of cardiopulmonary bypass...
December 20, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29350530/structural-variation-and-microrheological-properties-of-a-homogeneous-polysaccharide-from-wheat-germ
#11
Liyuan Yun, Tao Wu, Rui Liu, Kun Li, Min Zhang
A novel water-soluble polysaccharide (WGP) was purified from the crude wheat germ polysaccharide by Sephacryl S-500HRgel filtration. The molecular weight of WGP was determined as 4.89×106 Da and composed of arabinose, xylose, glucose, and galactose, with a molar ratio of 29.3%:42.7%:14.7%:13.3%, respectively. WGP was subjected for structural characterization by methylation analysis and 1D/2D nuclear magnetic resonance. WGP was mainly a backbone consisting of (1→4)-linked--D-Xylp (19.01%), (1→3, 4)-linked--D-Xylp (26...
January 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29337094/vitamin-d-attenuates-pressure-overload-induced-cardiac-remodeling-and-dysfunction-in-mice
#12
Liang Zhang, Xiao Yan, Jie Bai, Tesfaldet Habtemariam Hidru, Qing-Shan Wang, Hui-Hua Li
Vitamin D (VD) and its analogues play critical roles in metabolic and cardiovascular diseases. Recent studies have demonstrated that VD exerts a protective role in cardiovascular diseases. However, the beneficial effect of VD on pressure overload-induced cardiac remodeling and dysfunction and its underlying mechanisms are not fully elucidated. In this study, cardiac dysfunction and hypertrophic remodeling in mice were induced by pressure overload. Cardiac function was evaluated by echocardiography, and myocardial histology was detected by H&E and Masson's trichrome staining...
January 11, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29315079/safety-and-tolerability-of-spermidine-supplementation-in-mice-and-older-adults-with-subjective-cognitive-decline
#13
Claudia Schwarz, Slaven Stekovic, Miranka Wirth, Gloria Benson, Philipp Royer, Stephan J Sigrist, Thomas Pieber, Christopher Dammbrueck, Christoph Magnes, Tobias Eisenberg, Tobias Pendl, Jens Bohlken, Theresa Köbe, Frank Madeo, Agnes Flöel
Supplementation of spermidine, an autophagy-inducing agent, has been shown to protect against neurodegeneration and cognitive decline in aged animal models. The present translational study aimed to determine safety and tolerability of a wheat germ extract containing enhanced spermidine concentrations. In a preclinical toxicity study, supplementation of spermidine using this extract did not result in morbidities or changes in behavior in BALBc/Rj mice during the 28-days repeated-dose tolerance study. Post mortem examination of the mice organs showed no increase in tumorigenic and fibrotic events...
January 8, 2018: Aging
https://www.readbyqxmd.com/read/29304125/a-purified-fermented-extract-of-triticum-aestivum-has-lymphomacidal-activity-mediated-via-natural-killer-cell-activation
#14
Gustavo A Barisone, Robert T O'Donnell, Yunpeng Ma, Mastewal W Abuhay, Kathleen Lundeberg, Sonia Gowda, Joseph M Tuscano
Non-Hodgkin lymphoma (NHL) affects over 400,000 people in the United States; its incidence increases with age. Treatment options are numerous and expanding, yet efficacy is often limited by toxicity, particularly in the elderly. Nearly 70% patients eventually die of the disease. Many patients explore less toxic alternative therapeutics proposed to boost anti-tumor immunity, despite a paucity of rigorous scientific data. Here we evaluate the lymphomacidal and immunomodulatory activities of a protein fraction isolated from fermented wheat germ...
2018: PloS One
https://www.readbyqxmd.com/read/29290979/exacerbation-of-diabetic-cardiac-hypertrophy-in-ove26-mice-by-angiotensin-ii-is-associated-with-jnk-c-jun-mir-221-mediated-autophagy-inhibition
#15
Ling-Bo Qian, Sai-Zhi Jiang, Xiao-Qiang Tang, Jian Zhang, Ya-Qin Liang, Hai-Tao Yu, Jing Chen, Zheng Xu, Rui-Ming Liu, Bradley B Keller, Hong-Lei Ji, Lu Cai
Both diabetes and angiotensin II (Ang II) excess trigger cardiac remodeling and dysfunction, and diabetic cardiomyopathy. We hypothesized that cardiac hypertrophy associated with the development of diabetic cardiomyopathy is worsened by increased Ang II. Male type 1 diabetic OVE26 and wild-type mice were given Ang II (sc., 1.15 mg/kg, twice a day) for 14 days. Diabetes-induced cardiac dysfunction and hypertrophy was exacerbated by Ang II treatment as determined by echocardiography, wheat germ agglutinin staining and atrial natriuretic peptide...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29248314/the-effect-of-rebamipide-ophthalmic-suspension-on-ocular-surface-mucins-in-soft-contact-lens-wearers
#16
Chika Shigeyasu, Masakazu Yamada, Yoko Akune, Masaki Fukui
PURPOSE: To evaluate the changes in ocular surface mucins with 2%rebamipide ophthalmic suspension treatment in soft contact lens (SCL) wearers. Rebamipide suspension is a mucin secretagogue approved for the treatment of dry eye syndrome in Japan. In this study, the fluorescence intensity of wheat germ agglutinin conjugate of fluorescein (F-WGA) was used as a marker of membrane-associated mucins, and sialic acid concentration in tear fluids as a marker of secreted mucins. METHODS: Thirty-two eyes of 16 SCL wearers with discomfort were treated with rebamipide suspension at a dose of one drop in each eye four times daily for two weeks...
December 13, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29247959/triacylglycerol-hand-shape-profile-of-argan-oil-rapid-and-simple-uhplc-pda-esi-tof-ms-and-hptlc-methods-to-detect-counterfeit-argan-oil-and-argan-oil-based-products
#17
Giordana Pagliuca, Carlotta Bozzi, Francesca Romana Gallo, Giuseppina Multari, Giovanna Palazzino, Rita Porrà, Alessia Panusa
The marketing of new argan-based products is greatly increased in the last few years and consequently, it has enhanced the number of control analysis aimed at detecting counterfeit products claiming argan oil as a major ingredient. Argan oil is produced in Morocco and it is quite expensive. Two simple methods for the rapid screening of pure oil and argan-oil based products, focused on the analysis of the triacylglycerol profile, have been developed. A three-minute-run by UHPLC-PDA allows the identification of a pure argan oil, while the same run with the MS detector allows also the analysis of products containing the oil down to 0...
December 2, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29235215/cloning-free-template-dna-preparation-for-cell-free-protein-synthesis-via-two-step-pcr-using-versatile-primer-designs-with-short-3-utr
#18
Mika Nomoto, Yasuomi Tada
Cell-free protein synthesis (CFPS) systems largely retain the endogenous translation machinery of the host organism, making them highly applicable for proteomics analysis of diverse biological processes. However, laborious and time-consuming cloning procedures hinder progress with CFPS systems. Herein, we report the development of a rapid and efficient two-step polymerase chain reaction (PCR) method to prepare linear DNA templates for a wheat germ CFPS system. We developed a novel, effective short 3'-untranslated region (3'-UTR) sequence that facilitates translation...
December 13, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29228925/daily-activity-patterns-influence-retinal-morphology-signatures-of-selection-and-spectral-tuning-of-opsin-genes-in-colubrid-snakes
#19
E Hauzman, D M O Bonci, E Y Suárez-Villota, M Neitz, D F Ventura
BACKGROUND: Morphological divergences of snake retinal structure point to complex evolutionary processes and adaptations. The Colubridae family has a remarkable variety of retinal structure that can range from all-cone and all-rod to duplex (cone/rod) retinas. To explore whether nocturnal versus diurnal activity is responsible for constraints on molecular evolution and plays a role in visual opsin spectral tuning of colubrids, we carried out molecular evolution analyses of the visual opsin genes LWS, RH1, and SWS1 from 17 species and performed morphological analyses...
December 11, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/29217416/identification-of-target-proteins-of-clinical-immunity-to-plasmodium-falciparum-in-a-region-of-low-malaria-transmission
#20
Hirokazu Sakamoto, Satoru Takeo, Eizo Takashima, Kazutoyo Miura, Bernard N Kanoi, Takamasa Kaneko, Eun-Taek Han, Mayumi Tachibana, Kazuhiro Matsuoka, Jetsumon Sattabongkot, Rachanee Udomsangpetch, Tomoko Ishino, Takafumi Tsuboi
The target molecules of antibodies against falciparum malaria remain largely unknown. Recently we have identified multiple proteins as targets of immunity against Plasmodium falciparum using African serum samples. To investigate whether potential targets of clinical immunity differ with transmission intensity, we assessed immune responses in residents of low malaria transmission region in Thailand. Malaria asymptomatic volunteers (Asy: n=19) and symptomatic patients (Sym: n=21) were enrolled into the study...
December 4, 2017: Parasitology International
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