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https://www.readbyqxmd.com/read/28304151/cardiac-cell-proliferation-is-not-necessary-for-exercise-induced-cardiac-growth-but-required-for-its-protection-against-ischaemia-reperfusion-injury
#1
Yihua Bei, Siyi Fu, Xiangming Chen, Mei Chen, Qiulian Zhou, Pujiao Yu, Jianhua Yao, Hongbao Wang, Lin Che, Jiahong Xu, Junjie Xiao
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was utilized to induce cardiac physiological growth. Simultaneously, the antineoplastic agent 5-fluorouracil (5-FU), which acts during the S phase of the cell cycle, was given to mice via intraperitoneal injections. Using EdU and Ki-67 immunolabelling, we showed that exercise-induced cardiac cell proliferation was blunted by 5-FU...
March 17, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28282148/alterations-in-the-salivary-proteome-and-n-glycome-of-sj%C3%A3-gren-s-syndrome-patients
#2
Steven C Hall, Maria E Hassis, Katherine E Williams, Matthew E Albertolle, Akraporn Prakobphol, Andrew B Dykstra, Megan Laurance, Katherine Ona, Richard K Niles, Namrata Prasad, Matthew Gormley, Caroline Shiboski, Lindsey A Criswell, H Ewa Witkowska, Susan J Fisher
We used isobaric mass tagging (iTRAQ) and lectin affinity capture mass spectrometry (MS)-based workflows for global analyses of parotid saliva (PS) and whole saliva (WS) samples obtained from patients diagnosed with primary Sjögren's Syndrome (pSS) who were enrolled in the Sjögren's International Collaborative Clinical Alliance (SICCA) as compared with two control groups. The iTRAQ analyses revealed up- and down-regulation of numerous proteins that could be involved in the disease process (e.g., histones) or attempts to mitigate the ensuing damage (e...
March 24, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28280340/wheat-germ-agglutinin-conjugated-liposomes-incorporated-with-cardiolipin-to-improve-neuronal-survival-in-alzheimer-s-disease-treatment
#3
Yung-Chih Kuo, Che-Yu Lin, Jay-Shake Li, Yung-I Lou
Curcumin (CRM) and nerve growth factor (NGF) were entrapped in liposomes (LIP) with surface wheat germ agglutinin (WGA) to downregulate the phosphorylation of kinases in Alzheimer's disease (AD) therapy. Cardiolipin (CL)-conjugated LIP carrying CRM (CRM-CL/LIP) and also carrying NGF (NGF-CL/LIP) were used with AD models of SK-N-MC cells and Wistar rats after an insult with β-amyloid peptide (Aβ). We found that CRM-CL/LIP inhibited the expression of phosphorylated p38 (p-p38), phosphorylated c-Jun N-terminal kinase (p-JNK), and p-tau protein at serine 202 and prevented neurodegeneration of SK-N-MC cells...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28274431/artificial-neural-network-genetic-algorithm-to-optimize-wheat-germ-fermentation-condition-application-to-the-production-of-two-anti-tumor-benzoquinones
#4
Zi-Yi Zheng, Xiao-Na Guo, Ke-Xue Zhu, Wei Peng, Hui-Ming Zhou
Methoxy-ρ-benzoquinone (MBQ) and 2, 6-dimethoxy-ρ-benzoquinone (DMBQ) are two potential anticancer compounds in fermented wheat germ. In present study, modeling and optimization of added macronutrients, microelements, vitamins for producing MBQ and DMBQ was investigated using artificial neural network (ANN) combined with genetic algorithm (GA). A configuration of 16-11-1 ANN model with Levenberg-Marquardt training algorithm was applied for modeling the complicated nonlinear interactions among 16 nutrients in fermentation process...
July 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28249214/lectin-magnetic-separation-lms-for-isolation-of-toxoplasma-gondii-oocysts-from-concentrated-water-samples-prior-to-detection-by-microscopy-or-qpcr
#5
Jemere Bekele Harito, Andrew T Campbell, Kristoffer Relling Tysnes, J P Dubey, Lucy J Robertson
Although standard methods for analyzing water samples for the protozoan parasites Cryptosporidium spp. and Giardia duodenalis are available and widely used, equivalent methods for analyzing water samples for Toxoplasma gondii oocysts are lacking. This is partly due to the lack of a readily available, reliable immunomagnetic separation technique (IMS). Here we investigated the use of lectin-magnetic separation (LMS) for isolating T. gondii oocysts from water sample concentrates, with subsequent detection by microscopy or molecular methods...
February 21, 2017: Water Research
https://www.readbyqxmd.com/read/28242905/wheat-germ-stabilization-by-infrared-radiation
#6
Renato D Gili, Pablo M Palavecino, M Cecilia Penci, Marcela L Martinez, Pablo D Ribotta
Wheat germ has an important enzymatic activity, being lipases the enzymes which cause the highest impact in the reduction of shelf life. The objective of this study was to evaluate the effects of infrared radiation on wheat germ stabilization in an attempt to extend the shelf life. The effects of treatment time, gap (sample distance to IR emitters) and infrared radiation intensity on wheat germ were analyzed through response surface methodology. Final moisture content, final temperature, color of germ and germ oil quality parameters: free fatty acid content changes and total tocopherol content were the responses evaluated using a Box-Behnken design...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28237713/poly-l-glutamic-acid-drug-delivery-system-for-the-intravesical-therapy-of-bladder-cancer-using-wga-as-targeting-moiety
#7
C Apfelthaler, M Anzengruber, F Gabor, M Wirth
In the management of bladder cancer, surgical resection of the tumour is usually followed by intravesical instillation of immunomodulatives and/or chemotherapeutics. The purpose of this local intravesical therapy is to eliminate residual malignant cells after surgical intervention. The main limitation of a localised adjuvant therapy is the insufficient concentration of the active pharmaceutical ingredient (API) in malignant cells due to the unique structure of the human urothelium making it an exclusively hard to overcome barrier in the human body...
February 22, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28235802/a-cell-free-method-for-expressing-and-reconstituting-membrane-proteins-enables-functional-characterization-of-the-plant-receptor-like-protein-kinase-feronia
#8
Benjamin B Minkoff, Shin-Ichi Makino, Miyoshi Haruta, Emily T Beebe, Russell L Wrobel, Brian G Fox, Michael R Sussman
There are more than 600 receptor-like kinases (RLKs) in Arabidopsis, but due to challenges associated with the characterization of membrane proteins means, only a few have known biological functions. The plant RLK FERONIA is a peptide receptor and has been implicated in plant growth regulation, but little is known about its molecular mechanism of action. To investigate the properties of this enzyme, we used a cell-free wheat-germ based expression system in which mRNA encoding FERONIA was co-expressed with mRNA encoding the membrane scaffold protein variant MSP1D1...
February 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28218996/hypoxia-stimulated-membrane-trafficking-requires-t-plastin
#9
Marieke Wottawa, Stephanie Naas, Johannes Böttger, Gijsbert J van Belle, Wiebke Möbius, Natalia H Revelo, Doris Heidenreich, Melanie von Ahlen, Anke Zieseniss, Katharina Kröhnert, Susanne Lutz, Christof Lenz, Henning Urlaub, Silvio O Rizzoli, Dörthe M Katschinski
Traffic between the plasma membrane and the endomembrane compartments is an essential feature of eukaryotic cells. The secretory pathway sends cargoes from biosynthetic compartments to the plasma membrane. This is counterbalanced by a retrograde endocytotic route and is essential for cell homeostasis. Cells need to adapt rapidly to environmental challenges like the reduction of pO2 which, however, has not been analyzed in relation to membrane trafficking in detail. Therefore we determined changes in the plasma membrane trafficking in normoxia, hypoxia, and after reoxygenation METHODS: Membrane trafficking was analyzed by using the bulk membrane endocytosis marker FM 1-43, the newly developed membrane probe mCLING, wheat germ agglutinin as well as fluorescently labeled choleratoxin subunit B...
February 20, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28215876/in-vitro-lectin-binding-to-the-outer-surface-of-spirocerca-lupi-at-different-life-stages
#10
I Aroch, I Arogeti, A Marcovics, Y Spiegel, E Lavy
Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28203774/novel-chromatographic-methods-for-simultaneous-quantification-of-fish-and-wheat-germ-oils-mixture-in-pharmaceutical-dosage-forms
#11
Amira F El-Yazbi, Amr El-Hawiet
No abstract text is available yet for this article.
February 16, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28198918/artificial-diet-for-laboratory-rearing-of-condylorrhiza-vestigialis-guen%C3%A3-e-1854-lep-crambidae
#12
Lucas S Campos, Aloisio Coelho, José Roberto P Parra
The Brazilian Poplar Moth, Condylorrhiza vestigialis (Guenée), compromises the wood productivity of poplar trees (Populus sp.), mainly affecting the matchstick industry in southern Brazil. Considering the lack of information on rearing techniques for this insect, the objective of this study was to develop an artificial diet to rear C. vestigialis with biological characteristics similar to the wild insects. A properly diet will enable bio-ecological studies and biological control programs using the baculovirus Condylorrhiza vestigialis multiple nucleopolyhedrovirus (CvMNPV)...
February 9, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28157671/can-wheat-germ-have-a-beneficial-effect-on-human-health-a-study-protocol-for-a-randomised-crossover-controlled-trial-to-evaluate-its-health-effects
#13
André Moreira-Rosário, Helder Pinheiro, Conceição Calhau, Luís Filipe Azevedo
INTRODUCTION: Cardiovascular diseases (CVD) are the leading cause of mortality worldwide and diet is an important contributor to CVD risk. Thus, several food derivatives are being investigated for their beneficial impact on reducing cardiometabolic risk factors, either in risk groups or in healthy population as a preventive measure. Wheat germ is a food by-product with high nutritional value, especially as a concentrated source of dietary fibre and essential fatty acids, but its incorporation into the diet has been rare up to now...
November 10, 2016: BMJ Open
https://www.readbyqxmd.com/read/28143983/rim-pathway-mediated-alterations-in-the-fungal-cell-wall-influence-immune-recognition-and-inflammation
#14
Kyla S Ost, Shannon K Esher, Chrissy M Leopold Wager, Louise Walker, Jeanette Wagener, Carol Munro, Floyd L Wormley, J Andrew Alspaugh
Compared to other fungal pathogens, Cryptococcus neoformans is particularly adept at avoiding detection by innate immune cells. To explore fungal cellular features involved in immune avoidance, we characterized cell surface changes of the C. neoformans rim101Δ mutant, a strain that fails to organize and shield immunogenic epitopes from host detection. These cell surface changes are associated with an exaggerated, detrimental inflammatory response in mouse models of infection. We determined that the disorganized strain rim101Δ cell wall increases macrophage detection in a contact-dependent manner...
January 31, 2017: MBio
https://www.readbyqxmd.com/read/28132080/fvsnf1-the-sucrose-non-fermenting-protein-kinase-gene-of-fusarium-virguliforme-is-required-for-cell-wall-degrading-enzymes-expression-and-sudden-death-syndrome-development-in-soybean
#15
Kazi T Islam, Jason P Bond, Ahmad M Fakhoury
Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs). The sucrose non-fermenting protein kinase 1 gene (SNF1) is a key component of the glucose de-repression pathway in yeast, and a regulator of gene expression for CWDEs in some plant pathogenic fungi. To elucidate the functional role of the SNF1 homolog in F. virguliforme, FvSNF1 was disrupted using a split-marker strategy...
January 28, 2017: Current Genetics
https://www.readbyqxmd.com/read/28126680/testing-of-bioactive-nanovesicles-on-hepatotoxicity-of-atypical-antipsychotics-via-digital-holography
#16
Fatma Ozturk Kirbay, Caner Geyik, Emine Guler, Ozan Yesiltepe, Zinar Pinar Gumus, Dilek Odaci Demirkol, Hakan Coskunol, Suna Timur
Atypical antipsychotic drugs induce hepatic toxicity. Thus, it is of importance to eliminate the side effects of these drugs. Herein we describe the preparation of nanoemulsions with a dietary supplement; wheat germ oil (WGO), to ameliorate the liver damage induced by clozapine and olanzapine. THLE-2 cell line was used as a model to investigate the effects of these nanoemulsions on cell viability as well as antioxidative efficiency after antipsychotic insult. In this context, a conventional cell culture method; MTT was used along with a novel cellular imaging technique called digital holography (DH) to evaluate cell viability...
April 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28124793/fluoroacetamide-moieties-as-nmr-spectroscopy-probes-for-the-molecular-recognition-of-glcnac-containing-sugars-modulation-of-the-ch-%C3%AF-stacking-interactions-by-different-fluorination-patterns
#17
Luca Unione, Maria Alcalá, Begoña Echeverria, Sonia Serna, Ana Ardá, Antonio Franconetti, F Javier Cañada, Tammo Diercks, Niels Reichardt, Jesús Jiménez-Barbero
We herein propose the use of fluoroacetamide and difluoroacetamide moieties as sensitive tags for the detection of sugar-protein interactions by simple (1) H and/or (19) F NMR spectroscopy methods. In this process, we have chosen the binding of N,N'-diacetyl chitobiose, a ubiquitous disaccharide fragment in glycoproteins, by wheat-germ agglutinin (WGA), a model lectin. By using saturation-transfer difference (STD)-NMR spectroscopy, we experimentally demonstrate that, under solution conditions, the molecule that contained the CHF2 CONH- moiety is the stronger aromatic binder, followed by the analogue with the CH2 FCONH- group and the natural molecule (with the CH3 CONH- fragment)...
January 26, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28118713/production-of-rare-phyto-ceramides-from-abundant-food-plant-residues
#18
Mathias Reisberg, Norbert Arnold, Andrea Porzel, Reinhard H H Neubert, Birgit Dräger
Ceramides (Cers) are major components of the outermost layer of the skin, the stratum corneum, and play a crucial role in permeability barrier functions. Alterations in Cer composition causing skin diseases are compensated with semisynthetic skin-identical Cers. Plants constitute new resources for Cer production as they contain glucosylceramides (GluCers) as major components. GluCers were purified from industrial waste plant materials, apple pomace (Malus domestica), wheat germs (Triticum sp.), and coffee grounds (Coffea sp...
February 13, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28099512/age-dependent-oxidative-dna-damage-does-not-correlate-with-reduced-proliferation-of-cardiomyocytes-in-humans
#19
Yanhui Huang, Haifa Hong, Minghui Li, Jinfen Liu, Chuan Jiang, Haibo Zhang, Lincai Ye, Jinghao Zheng
BACKGROUND: Postnatal human cardiomyocyte proliferation declines rapidly with age, which has been suggested to be correlated with increases in oxidative DNA damage in mice and plays an important role in regulating cardiomyocyte proliferation. However, the relationship between oxidative DNA damage and age in humans is unclear. METHODS: Sixty right ventricular outflow myocardial tissue specimens were obtained from ventricular septal defect infant patients during routine congenital cardiac surgery...
2017: PloS One
https://www.readbyqxmd.com/read/28089547/antibody-profiles-to-wheat-germ-cell-free-system-synthesized-plasmodium-falciparum-proteins-correlate-with-protection-from-symptomatic-malaria-in-uganda
#20
Bernard N Kanoi, Eizo Takashima, Masayuki Morita, Michael T White, Nirianne M Q Palacpac, Edward H Ntege, Betty Balikagala, Adoke Yeka, Thomas G Egwang, Toshihiro Horii, Takafumi Tsuboi
The key targets of protective antibodies against Plasmodium falciparum remain largely unknown. In this study, we determined immunoreactivity to 1827 recombinant proteins derived from 1565 genes representing ∼30% of the entire P. falciparum genome, for identification of novel malaria vaccine candidates. The recombinant proteins were expressed by wheat germ cell-free system, a platform that can synthesize quality plasmodial proteins that elicit biologically active antibodies in animals. Sera were obtained from indigenous residents of a malaria endemic region in Northern Uganda who were enrolled at the start of a rainy season and prospectively monitored for symptomatic malaria episodes for a year...
January 12, 2017: Vaccine
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