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https://www.readbyqxmd.com/read/29144022/collagen-a-review-on-its-sources-and-potential-cosmetic-applications
#1
REVIEW
María Isabela Avila Rodríguez, Laura G Rodríguez Barroso, Mirna Lorena Sánchez
Collagen is a fibrillar protein that conforms the conjunctive and connective tissues in the human body, essentially skin, joints, and bones. This molecule is one of the most abundant in many of the living organisms due to its connective role in biological structures. Due to its abundance, strength and its directly proportional relation with skin aging, collagen has gained great interest in the cosmetic industry. It has been established that the collagen fibers are damaged with the pass of time, losing thickness and strength which has been strongly related with skin aging phenomena [Colágeno para todo...
November 16, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/29124019/a-protocol-for-gene-expression-analysis-of-chondrocytes-from-bovine-osteochondral-plugs-used-for-biotribological-applications
#2
Christoph Bauer, Eugenia Niculescu-Morzsa, Stefan Nehrer
RNA isolation from human or animal cartilage tissue is necessary when performing mechanical or biotribological applications. Despite no influence on the cells and no alterations in gene expression patterns, enzymatic digestion of tissues should be avoided as it's known that the expression of collagen 2 can be effected (Hayman et al., 2006 [1]). After mechanical or biotribological tests alternative options with an immediate disruption of the tissue should be contemplated. To obtain RNA, different tissue homogenization and disruption methods are available on the market (Yu et al...
2017: MethodsX
https://www.readbyqxmd.com/read/29100004/autologous-chondrocyte-implantation-in-osteoarthritic-surroundings-tnf%C3%AE-and-its-inhibition-by-adalimumab-in-a-knee-specific-bioreactor
#3
Robert Ossendorff, Sibylle Grad, Martin J Stoddart, Mauro Alini, Hagen Schmal, Norbert Südkamp, Gian M Salzmann
BACKGROUND: Autologous chondrocyte implantation (ACI) fails in up to 20% of cases. Advanced intra-articular degeneration paired with an inflammatory environment may be closely related to implantation failure. Certain cytokines have been identified to play a major role during early osteoarthritis. PURPOSE: To investigate the effects of tumor necrosis factor α (TNFα) and its potential inhibition by adalimumab on cartilage regeneration in an in vitro model of ACI...
November 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29024719/calcification-or-not-this-is-the-question-a-one-year-study-of-bovine-pericardial-vascular-patches-cardiocel%C3%A2-in-minipigs
#4
Aida Salameh, Wiebke Greimann, David Vondrys, Martin Kostelka
Objectives A main problem with bioprosthesis used for surgical correction of congenital cardiac malformation is their tendency to shrink and to calcify. Recently, a new material i.e. de-cellularised bovine pericardium (CardioCel(®)) was introduced in clinics. It was proposed that this new patch material should not calcify and should be particularly suitable for the correction of vascular defects in inborn cardiac diseases. The aim of our chronic minipig study was to evaluate the performance of CardioCel(®) patches implanted in aortic and pulmonary artery position, respectively...
October 9, 2017: Seminars in Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28979694/polyene-phosphatidylcholine-inhibited-the-inflammatory-response-in-lps-stimulated-macrophages-and-ameliorated-the-adjuvant-induced-rat-arthritis
#5
Wei Pan, Wen-Ting Hao, Hui-Wen Xu, Su-Ping Qin, Xiang-Yang Li, Xiao-Mei Liu, Fen-Fen Sun, Hui Li, Ren-Xian Tang, Kui-Yang Zheng
This study sought to investigate the anti-inflammatory effect of Polyene Phosphatidylcholine (PPC), a clinical drug that is used to treat hepatopathy, on lipopolysaccharide (LPS)-stimulated macrophages and on bovine collagen II-induced arthritis (CIA) rats. In stimulated primary and Raw264.7 macrophages by LPS, PPC significantly down-regulated the relative expression of mRNA such as IL-6, TNF-α, TLR-2, TLR-4, MyD88, and NF-κB while up-regulated IL-10 and TGF-β expression. Moreover, the concentration of IL-6, TNF-α, IL-10, and TGF-β in the cultured supernatants showed the similar tendency with their mRNA alterations...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28970490/mast-cell-intervertebral-disc-cell-interactions-regulate-inflammation-catabolism-and-angiogenesis-in-discogenic-back-pain
#6
Matthew G Wiet, Andrew Piscioneri, Safdar N Khan, Megan N Ballinger, Judith A Hoyland, Devina Purmessur
Low back pain (LBP) is a widespread debilitating disorder of significant socio-economic importance and intervertebral disc (IVD) degeneration has been implicated in its pathogenesis. Despite its high prevalence the underlying causes of LBP and IVD degeneration are not well understood. Recent work in musculoskeletal degenerative diseases such as osteoarthritis have revealed a critical role for immune cells, specifically mast cells in their pathophysiology, eluding to a potential role for these cells in the pathogenesis of IVD degeneration...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28934882/development-of-a-cartilage-shear-damage-model-to-investigate-the-impact-of-surface-injury-on-chondrocytes-and-extracellular-matrix-wear
#7
Robert L Trevino, Carol A Pacione, Anne-Marie Malfait, Susan Chubinskaya, Markus A Wimmer
Background Many i n vitro damage models investigate progression of cartilage degradation after a supraphysiologic, compressive impact at the surface and do not model shear-induced damage processes. Models also neglect the response to uninterrupted tribological stress after damage. It was hypothesized that shear-induced removal of the superficial zone would accelerate matrix degradation when damage was followed by continued load and articulation. Methods Bovine cartilage underwent a 5-day test. Shear-damaged samples experienced 2 days of damage induction with articulation against polyethylene and then continued articulation against cartilage (CoC), articulation against metal (MoC), or rest as free-swelling control (FSC)...
October 2017: Cartilage
https://www.readbyqxmd.com/read/28901397/beneficial-effect-of-20%C3%A2-hydroxyecdysone-exerted-by-modulating-antioxidants-and-inflammatory-cytokine-levels-in-collagen%C3%A2-induced-arthritis-a-model-for-rheumatoid-arthritis
#8
You Sun, De-Li Zhao, Zi-Xuan Liu, Xue-Hui Sun, Yang Li
Rheumatoid arthritis is a chronic autoimmune disease characterized by an elevated synovial inflammatory response, with destruction or erosion of articular cartilage in major joints. The aim of the present study was to examine whether 20‑hydroxyecdysone (HES) is able to ameliorate oxidative stress and inflammatory responses in a collagen‑induced rheumatoid arthritis (CIA) rat model. A total of 40 healthy male rats were selected arbitrarily and separated into four groups. Rats treated with saline served as a control (group I), rats subjected to CIA induction by intradermal injection of bovine collagen II type served as the induced group (group II), while rats induced with CIA and administered with 10 and 20 mg/kg bodyweight HES for 28 days served as treatment groups (groups III and IV)...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28892654/single-stage-cartilage-repair-using-platelet-rich-fibrin-scaffolds-with-autologous-cartilaginous-grafts
#9
Chin-Chean Wong, Chih-Hwa Chen, Wing P Chan, Li-Hsuan Chiu, Wei-Pin Ho, Fon-Jou Hsieh, You-Tzung Chen, Tsung-Lin Yang
BACKGROUND: To avoid complicated procedures requiring in vitro chondrocyte expansion for cartilage repair, the development of a culture-free, 1-stage approach combining platelet-rich fibrin (PRF) and autologous cartilage grafts may be the solution. PURPOSE: To develop a feasible 1-step procedure to combine PRF and autologous cartilage grafts for articular chondral defects. STUDY DESIGN: Controlled laboratory study Methods: The chemotactic effects of PRF on chondrocytes harvested from the primary culture of rabbit cartilage were evaluated in vitro and ex vivo...
November 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28892196/effect-of-biomechanical-stress-on-endogenous-antioxidant-networks-in-bovine-articular-cartilage
#10
Rita Issa, Michael Boeving, Michael Kinter, Timothy M Griffin
Mechanosensitve pathways in chondrocytes are essential for maintaining articular cartilage homeostasis. Traumatic loading increases cartilage oxidation and causes cell death and osteoarthritis. However, sub-lethal doses of the pro-oxidant molecule tert-Butyl hydroperoxide (tBHP) protects against loading-induced chondrocyte death. We hypothesized that compressive cyclic loading at moderate strains (<20%) causes sub-lethal cartilage oxidation that induces an adaptive increase in the endogenous antioxidant defense network...
September 11, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28870737/chondrocyte-primary-cilium-is-mechanosensitive-and-responds-to-low-intensity-ultrasound-by-altering-its-length-and-orientation
#11
Anuradha Subramanian, Gaurav Budhiraja, Neety Sahu
The primary cilium has been implicated in mechanotransduction, however, its mechanosensory role in transducing signals under low-intensity ultrasound (LIUS) which has the potential to repair fractures and cartilage, remains uninvestigated. This study examined the impact of continuous-wave US, at the cell resonance frequency of 5.0MHz and pressure amplitude of either 14 or 60kPa, on the incidence, length and orientation of primary cilium in bovine articular chondrocytes. Visualization of primary cilium with acetylated α-tubulin staining demonstrated that the primary cilium was elongated, bent under US and these changes were reversible...
October 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28852032/orientation-anisotropy-of-quantitative-mri-relaxation-parameters-in-ordered-tissue
#12
Nina Hänninen, Jari Rautiainen, Lassi Rieppo, Simo Saarakkala, Mikko Johannes Nissi
In highly organized tissues, such as cartilage, tendons and white matter, several quantitative MRI parameters exhibit dependence on the orientation of the tissue constituents with respect to the main imaging magnetic field (B0). In this study, we investigated the dependence of multiple relaxation parameters on the orientation of articular cartilage specimens in the B0. Bovine patellar cartilage-bone samples (n = 4) were investigated ex vivo at 9.4 Tesla at seven different orientations, and the MRI results were compared with polarized light microscopy findings on specimen structure...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821957/co-culture-of-chondrons-and-mesenchymal-stromal-cells-reduces-the-loss-of-collagen-vi-and-improves-extracellular-matrix-production
#13
H A Owida, T De Las Heras Ruiz, A Dhillon, Y Yang, N J Kuiper
Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of collagen VI in articular cartilage. Collagen VI anchors the chondrocyte to the PCM. It has been suggested that co-culture of chondrons with mesenchymal stromal cells (MSCs) might enhance extracellular matrix (ECM) production. This co-culture study investigates whether MSCs help to preserve the PCM and increase ECM production...
August 19, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28804219/damps-synergize-with-cytokines-or-fibronectin-fragment-on-inducing-chondrolysis-but-lose-effect-when-acting-alone
#14
Lei Ding, Joseph A Buckwalter, James A Martin
OBJECTIVE AND DESIGN: To investigate whether endogenous damage-associated molecular patterns (DAMPs) or alarmins originated from mitochondria or nucleus stimulates inflammatory response in articular chondrocytes to cause chondrolysis which leads to cartilage degradation featured in posttraumatic osteoarthritis (PTOA). MATERIALS: Primary cultures of bovine or human chondrocytes isolated from cartilage of weight-bearing joints. TREATMENT: Chondrocytes were subjected to mitochondrial DAMPs (MTDs) or HMGB1, a nuclear DAMP (NuD), with or without the presence of an N-terminal 29 kDa fibronectin fragment (Fn-f) or proinflammatory cytokines (IL-1β and TNF-α)...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28803113/how-a-decreased-fibrillar-interconnectivity-influences-stiffness-and-swelling-properties-during-early-cartilage-degeneration
#15
Mieke Nickien, Ashvin Thambyah, Neil D Broom
OBJECTIVE: The functional coupling between the fibrillar network and the high-swelling proteoglycans largely determines the mechanical properties of the articular cartilage matrix. The objective of this new study was to show specifically how changes in fibrillar interconnectivity arising from early cartilage degeneration influence transverse stiffness and swelling properties at the tissue level. DESIGN: Radial zone transverse layers of cartilage matrix were obtained from intact and mildly degenerate bovine patellae...
August 3, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28800220/the-secret-life-of-collagen-temporal-changes-in-nanoscale-fibrillar-pre-strain-and-molecular-organization-during-physiological-loading-of-cartilage
#16
Sheetal R Inamdar, David P Knight, Nicholas J Terrill, Angelo Karunaratne, Fernando Cacho-Nerin, Martin M Knight, Himadri S Gupta
Articular cartilage is a natural biomaterial whose structure at the micro- and nanoscale is critical for healthy joint function and where degeneration is associated with widespread disorders such as osteoarthritis. At the nanoscale, cartilage mechanical functionality is dependent on the collagen fibrils and hydrated proteoglycans that form the extracellular matrix. The dynamic response of these ultrastructural building blocks at the nanoscale, however, remains unclear. Here we measure time-resolved changes in collagen fibril strain, using small-angle X-ray diffraction during compression of bovine and human cartilage explants...
October 24, 2017: ACS Nano
https://www.readbyqxmd.com/read/28777076/cnt-decellularized-cartilage-hybrids-for-tissue-engineering-applications
#17
Toktam Ghassemi, Nasser Saghatolslami, Maryam M Matin, Reza Gheshlaghi, Ali Moradi
An optimized scaffold with adaptable architectural and biochemical properties is a goal for articular cartilage (AC) repair. A mechanically enhanced decellularized AC can be an optimistic ECM-derived scaffold. In this study, reinforced decellularized bovine AC was evaluated as a potential scaffold for cartilage repair applications. Individually dispersed single-wall carbon nanotubes (CNTs) were incorporated into chemically decellularized bovine AC samples. The mechanical and thermodynamic properties as well as the biocompatibility of the samples were evaluated by a compressive test, SEM, AFM, FTIR, TGA, DSC and a resazurin test...
October 25, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28773080/estimation-of-articular-cartilage-surface-roughness-using-gray-level-co-occurrence-matrix-of-laser-speckle-image
#18
Doaa Youssef, Hatem El-Ghandoor, Hamed Kandel, Jala El-Azab, Salah Hassab-Elnaby
The application of He-Ne laser technologies for description of articular cartilage degeneration, one of the most common diseases worldwide, is an innovative usage of these technologies used primarily in material engineering. Plain radiography and magnetic resonance imaging are insufficient to allow the early assessment of the disease. As surface roughness of articular cartilage is an important indicator of articular cartilage degeneration progress, a safe and noncontact technique based on laser speckle image to estimate the surface roughness is provided...
June 28, 2017: Materials
https://www.readbyqxmd.com/read/28763685/viscoelasticity-of-articular-cartilage-analysing-the-effect-of-induced-stress-and-the-restraint-of-bone-in-a-dynamic-environment
#19
Bernard M Lawless, Hamid Sadeghi, Duncan K Temple, Hemeth Dhaliwal, Daniel M Espino, David W L Hukins
The aim of this study was to determine the effect of the induced stress and restraint provided by the underlying bone on the frequency-dependent storage and loss stiffness (for bone restraint) or modulus (for induced stress) of articular cartilage, which characterise its viscoelasticity. Dynamic mechanical analysis has been used to determine the frequency-dependent viscoelastic properties of bovine femoral and humeral head articular cartilage. A sinusoidal load was applied to the specimens and out-of-phase displacement response was measured to determine the phase angle, the storage and loss stiffness or modulus...
November 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28752866/alginate-based-hydrogels-functionalised-at-the-nanoscale-using-layer-by-layer-assembly-for-potential-cartilage-repair
#20
P Gentile, C Ghione, A M Ferreira, A Crawford, P V Hatton
Injuries to articular cartilage are frequently difficult to repair, in part because of the poor regenerative capacity of this tissue. To date, no successful system for complete regeneration of the most challenging cartilage defects has been demonstrated. The aim of this work was to develop functionalised hydrogels at the nanoscale by Layer-by-Layer (LbL) assembly to promote cartilage healing. Hydrogels, based on sodium alginate (NaAlg) and gelatin (G), were prepared by an external gelation method consisting of CaCl2 diffusion and genipin addition for G crosslinking...
August 22, 2017: Biomaterials Science
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