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https://www.readbyqxmd.com/read/29761549/sprifermin-treatment-enhances-cartilage-integration-in-an-in-vitro-repair-model
#1
Mackenzie L Sennett, Gregory R Meloni, Alexandra J E Farran, Hans Guehring, Robert L Mauck, George R Dodge
Cartilage integration remains a clinical challenge for treatment of focal articular defects. Cartilage exhibits limited healing capacity that declines with tissue maturation. Many approaches have been investigated for their ability to stimulate healing of mature cartilage or integration of repair tissue or tissue-engineered constructs with native cartilage. Growth factors present in immature tissue may enhance chondrogenesis and promote integrative repair of cartilage defects. In this study, we assessed the role of one such factor, fibroblast growth factor 18 (FGF18)...
May 14, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29758546/chondrogenic-properties-of-collagen-type-xi-a-component-of-cartilage-extracellular-matrix
#2
Ang Li, Yiyong Wei, Clark Hung, Gordana Vunjak-Novakovic
Cartilage extracellular matrix (ECM) has been used for promoting tissue engineering. However, the exact effects of ECM on chondrogenesis and the acting mechanisms are not well understood. In this study, we investigated the chondrogenic effects of cartilage ECM on human mesenchymal stem cells (MSCs) and identified the contributing molecular components. To this end, a preparation of articular cartilage ECM was supplemented to pellets of chondrogenically differentiating MSCs, pellets of human chondrocytes, and bovine articular cartilage explants to evaluate the effects on cell proliferation and the production of cartilaginous matrix...
May 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29752100/squid-type-ii-collagen-as-a-novel-biomaterial-isolation-characterization-immunogenicity-and-relieving-effect-on-degenerative-osteoarthritis-via-inhibiting-stat1-signaling-in-pro-inflammatory-macrophages
#3
Meilu Dai, Xin Liu, Nanping Wang, Jiao Sun
Collagen from marine organisms has a broad prospect in biomedical field, yet the knowledge on marine-derived type II collagen is rare. Herein, a novel type II collagen was successfully isolated from squid cartilage for the first time. After being characterized, the immunogenicity of squid type II collagen (SCII) was evaluated and compared with that of bovine type II collagen (BCII). Then investigations were further conducted for the impacts of SCII on pro-inflammatory macrophages and macrophage chemotaxis. The degenerative osteoarthritis (OA) -relieving effects of SCII were explored using OA rat model in vivo...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29714398/impact-of-human-platelet-lysate-on-the-expansion-and-chondrogenic-capacity-of-cultured-human-chondrocytes-for-cartilage-cell-therapy
#4
J G Sykes, J H Kuiper, J B Richardson, S Roberts, K T Wright, N J Kuiper
High hopes have been pinned on regenerative medicine strategies in order to prevent the progression of cartilage damage to osteoarthritis, particularly by autologous chondrocyte implantation (ACI). The loss of chondrocyte phenotype during in vitro monolayer expansion, a necessary step to obtain sufficient cell numbers, may be a key limitation in ACI. In this study, it was determined whether a shorter monolayer expansion approach could improve chondrogenic differentiation. The effects of two supplement types, foetal bovine serum (FBS) and Stemulate™ (a commercial source of human platelet lysate), on the expansion and re-differentiation potential of human chondrocytes, isolated from five individuals, were compared...
May 1, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29693628/influence-of-mechanical-unloading-on-articular-chondrocyte-dedifferentiation
#5
Simon L Wuest, Martina Caliò, Timon Wernas, Samuel Tanner, Christina Giger-Lange, Fabienne Wyss, Fabian Ille, Benjamin Gantenbein, Marcel Egli
Due to the limited self-repair capacity of articular cartilage, the surgical restoration of defective cartilage remains a major clinical challenge. The cell-based approach, which is known as autologous chondrocyte transplantation (ACT), has limited success, presumably because the chondrocytes acquire a fibroblast-like phenotype in monolayer culture. This unwanted dedifferentiation process is typically addressed by using three-dimensional scaffolds, pellet culture, and/or the application of exogenous factors...
April 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29691361/salvianolic-acid-b-suppresses-inflammatory-mediator-levels-by-downregulating-nf-%C3%AE%C2%BAb-in-a-rat-model-of-rheumatoid-arthritis
#6
Zeng-Bing Xia, Yong-Jian Yuan, Qiang-Hua Zhang, Heng Li, Ji-Lin Dai, Ji-Kang Min
BACKGROUND Salvianolic acid B (SB) is a major active phyto-component of the plant Radix Salvia miltiorrhiza, which is traditionally used to treat joint pain and arthritis. The present study examined the anti-rheumatoid arthritis efficacy of SB on collagen-induced rheumatoid arthritis (CIA) in a rat model. MATERIAL AND METHODS Forty-eight rats were divided into 4 groups: Control rats treated with saline (Group I), rats subjected to CIA induction by intradermal injection of bovine collagen II type at the tail (Group II), and rats subjected to CIA and supplemented with either 20 or 40 mg/kg of SB for 28 days (group III or IV)...
April 25, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29690771/non-destructive-spectroscopic-assessment-of-high-and-low-weight-bearing-articular-cartilage-correlates-with-mechanical-properties
#7
James P Karchner, Farzad Yousefi, Stephanie R Bitman, Kurosh Darvish, Nancy Pleshko
Objective Autologous articular cartilage (AC) harvested for repair procedures of high weight bearing (HWB) regions of the femoral condyles is typically obtained from low weight bearing (LWB) regions, in part due to the lack of non-destructive techniques for cartilage composition assessment. Here, we demonstrate that infrared fiber optic spectroscopy can be used to non-destructively evaluate variations in compositional and mechanical properties of AC across LWB and HWB regions. Design AC plugs ( N = 72) were harvested from the patellofemoral groove of juvenile bovine stifle joints, a LWB region, and femoral condyles, a HWB region...
April 1, 2018: Cartilage
https://www.readbyqxmd.com/read/29687923/quantitative-susceptibility-mapping-of-articular-cartilage-ex-vivo-findings-at-multiple-orientations-and-following-different-degradation-treatments
#8
Olli Nykänen, Lassi Rieppo, Juha Töyräs, Ville Kolehmainen, Simo Saarakkala, Karin Shmueli, Mikko J Nissi
PURPOSE: We investigated the feasibility of quantitative susceptibility mapping (QSM) for assessing degradation of articular cartilage by measuring ex vivo bovine cartilage samples subjected to different degradative treatments. Specimens were scanned at several orientations to study if degradation affects the susceptibility anisotropy. T2*-mapping, histological stainings, and polarized light microscopy were used as reference methods. Additionally, simulations of susceptibility in layered geometry were performed...
April 24, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29679855/rutin-and-rutin-conjugated-gold-nanoparticles-ameliorate-collagen-induced-arthritis-in-rats-through-inhibition-of-nf-%C3%AE%C2%BAb-and-inos-activation
#9
Anum Gul, Bimal Kunwar, Maryam Mazhar, Shaheen Faizi, Dania Ahmed, Muhammad Raza Shah, Shabana U Simjee
Numerous studies have suggested that nuclear factor-κB (NF-κB) and inducible nitric oxide synthase (iNOS) are important mediators of inflammatory response in human and animal models of arthritis. Besides, oxidative stress markers, nitric oxide (NO) and peroxide (PO) are also major contributors in the pathogenesis of rheumatoid arthritis (RA). Over expression of these inflammatory mediators leads to the extracellular matrix degradation, and excessive cartilage and bone resorption, ultimately leading to the irreversible damage to joints...
April 18, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29663489/high-glucose-induced-excessive-reactive-oxygen-species-promote-apoptosis-through-mitochondrial-damage-in-rat-cartilage-endplate-cells
#10
Zengxin Jiang, Wei Lu, Qingmin Zeng, Defang Li, Lei Ding, Jingping Wu
PURPOSE: Diabetes mellitus (DM) is an important factor in intervertebral disc degeneration (IDD). Apoptosis of cartilage endplate (CEP) cells is one of the initiators of IDD. However, the effects of high glucose on CEP cells are still unknown. Therefore, we conducted the present study to evaluate the effects of high glucose on CEP cells and to identify the mechanisms of those effects. METHODS: Rat CEP cells were isolated and cultured in 10% foetal bovine serum (FBS, normal control) or high-glucose medium (10% FBS + 0...
April 16, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29660649/tribological-evaluation-of-biomedical-polycarbonate-urethanes-against-articular-cartilage
#11
Yusuf Kanca, Piers Milner, Daniele Dini, Andrew A Amis
This research investigated the in-vitro wear and friction performance of polycarbonate urethane (PCU) 80A as they interact with articular cartilage, using a customised multidirectional pin-on-plate tester. Condyles were articulated against PCU 80A discs (Bionate® I and Bionate® II) (configuration 1) and the results arising from these tests were compared to those recorded during the sliding of PCU pins against cartilage plates (configuration 2). Configuration 1 produced steadily increasing coefficient of friction (COF) (up to 0...
April 3, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29628132/biomechanics-of-osteochondral-impact-with-cushioning-and-graft-insertion-cartilage-damage-is-correlated-with-delivered-energy
#12
Alvin W Su, Yunchan Chen, Yao Dong, Dustin H Wailes, Van W Wong, Albert C Chen, Shengqiang Cai, William D Bugbee, Robert L Sah
Articular cartilage is susceptible to impact injury. Impact may occur during events ranging from trauma to surgical insertion of an OsteoChondral Graft (OCG) into an OsteoChondral Recipient site (OCR). To evaluate energy density as a mediator of cartilage damage, a specialized drop tower apparatus was used to impact adult bovine samples while measuring contact force, cartilage surface displacement, and OCG advancement. When a single impact was applied to an isolated (non-inserted) OCG, force and surface displacement each rose monotonically and then declined...
March 30, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29589167/cartilage-metabolism-is-modulated-by-synovial-fluid-through-metalloproteinase-activity
#13
Eric Y Sun, Allison K M Fleck, Ahmad E Abu-Hakmeh, Alexandra Kotsakis, Garrett R Leonard, Leo Q Wan
Synovial fluid (SF) contains various cytokines that regulate chondrocyte metabolism and is dynamically associated with joint disease. The objective of this study was to investigate the effects of diluted normal SF on catabolic metabolism of articular cartilage under inflammatory conditions. For this purpose, SF was isolated from healthy bovine joints, diluted, and added to cartilage explant cultures stimulated with interleukin-1 (IL-1) for 12 days. The kinetic release of sulfated glycosaminoglycan (sGAG) and collagen, as well as nitric oxide and gelatinase matrix metalloproteinases were analyzed in the supernatant...
March 27, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29575932/ecm-based-injectable-thermo-sensitive-hydrogel-on-the-recovery-of-injured-cartilage-induced-by-osteoarthritis
#14
Yitong Li, Jie Cao, Shangcong Han, Yan Liang, Tingting Zhang, Han Zhao, Libin Wang, Yong Sun
Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage. We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM). The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated...
March 25, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29575101/adherent-agarose-mold-cultures-an-in-vitro-platform-for-multi-factorial-assessment-of-passaged-chondrocyte-redifferentiation
#15
Justin Parreno, Vanessa J Bianchi, Corey Sermer, Suresh C Regmi, David Backstein, Tannin A Schmidt, Rita A, Kandel
Generating the best possible bioengineered cartilage from passaged chondrocytes requires culture condition optimization. In this study the use of adherent agarose mold (adAM) cultures to support redifferentiation of passaged twice (P2) chondrocytes and serve as a scalable platform to assess the effect of growth factor combinations on proteoglycan accumulation by cells was examined. By 2 days in adAM culture, bovine P2 cells were partially redifferentiated as demonstrated by regression of actin-based dedifferentiation signalling and fibroblast matrix and contractile gene expression...
March 25, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29561494/creation-of-experimental-human-nose-model-with-lyophilized-and-decellularized-bovine-cartilage-xenograft
#16
Can Ilker Demir, Emrah Kağan Yaşar, Kivanç Davun, Aykut Gök, Ceyhun Uzun, Murat Şahin Alagöz
The nose anatomy is a functional and aesthetically important organ because of its three-dimensional structure, visible location in the face region, and its connection with the respiratory tract. Aesthetic and reconstructive nasal surgery requires correction of deformations in cartilage and bone structures as well as preservation of the natural connections between all subunits. The minimal mistake made can result in functional or aesthetically bad results. In this study, the authors aimed to create an experimental nose model that help aesthetic and reconstructive nose surgery operations...
March 20, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29537109/cdc42-regulates-the-expression-of-superficial-zone-molecules-in-part-through-the-actin-cytoskeleton-and-myocardin-related-transcription-factor-a
#17
Elizabeth Delve, Justin Parreno, Vivian Co, Po-Han Wu, Jasmine Chong, Matteo Di Scipio, Rita A Kandel
Osteoarthritis (OA) is a degenerative disease that initially manifests as loss of the superficial zone (SZ) of articular cartilage. SZ chondrocytes (SZC) differ in morphology from other chondrocytes as they are elongated and oriented parallel to the tissue surface. Proteoglycan 4 (PRG4) and tenascin C (TNC) are molecules expressed by SZC, which have been shown to be chondroprotective. Identification of the signalling pathway(s) regulating expression of SZ molecules may lead to a therapeutic target that can be used to delay or prevent the onset of OA...
March 14, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29527359/phospholipid-vesicles-in-media-for-tribological-studies-against-live-cartilage
#18
Teresa Veselack, Gregoire Aldebert, Ana-Maria Trunfio-Sfarghiu, Thomas M Schmid, Michel P Laurent, Markus A Wimmer
Introduction: Pre-clinical testing of hemiarthroplasty devices requires that the tribological conditions present in vivo with live cartilage be closely duplicated. A current limitation in the tribological testing of live cartilage involves the use of cell-culture media as lubricant. Study Aim: to develop and test a new hyaluronan-phospholipid based medium (HA-phospholipid medium) that combines the rheological and frictional properties of synovial fluid with the nourishing properties of culture media to keep cells alive...
2018: Lubricants
https://www.readbyqxmd.com/read/29500981/a-new-mechanical-indentation-framework-for-functional-assessment-of-articular-cartilage
#19
Zohreh Arabshahi, Isaac Oluwaseun Afara, Hayley Ruscoe Moody, Karsten Schrobback, Jamal Kashani, Nadine Fischer, Adekunle Oloyede, Travis Jacob Klein
The conventional mechanical properties of articular cartilage, such as compressive stiffness, have been shown to have limited capacity to distinguish visually normal from degraded cartilage samples. In this study, a new mechanical indentation framework for assessing functional properties of articular cartilage during loading/unloading, i.e. deformation and recovery, was established. The capacity of a ring-shaped indenter integrated with an ultrasound transducer to distinguish mechanically intact from proteoglycan-depleted tissue was investigated...
May 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29489829/trophic-effects-of-adipose-tissue-derived-and-bone-marrow-derived-mesenchymal-stem-cells-enhance-cartilage-generation-by-chondrocytes-in-co-culture
#20
M M Pleumeekers, L Nimeskern, J L M Koevoet, M Karperien, K S Stok, G J V M van Osch
AIMS: Combining mesenchymal stem cells (MSCs) and chondrocytes has great potential for cell-based cartilage repair. However, there is much debate regarding the mechanisms behind this concept. We aimed to clarify the mechanisms that lead to chondrogenesis (chondrocyte driven MSC-differentiation versus MSC driven chondroinduction) and whether their effect was dependent on MSC-origin. Therefore, chondrogenesis of human adipose-tissue-derived MSCs (hAMSCs) and bone-marrow-derived MSCs (hBMSCs) combined with bovine articular chondrocytes (bACs) was compared...
2018: PloS One
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