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P Gul, O T Harorlı, I B Ocal, Z Ergin, C Barutcigil
BACKGROUND AND PURPOSE: Discoloration of resin-based composites is a commonly encountered problem, and bleaching agents may be used for the therapy of the existing discoloration. The purpose of this study was to investigate in vitro color recovery effect of different bleaching systems on the heavily discolored composite resin. MATERIALS AND METHODS: Fifty disk-shaped dental composite specimens were prepared using A2 shade nanohybrid universal composite resin (3M ESPE Filtek Z550, St...
October 2017: Nigerian Journal of Clinical Practice
Qiuli Wang, Xin Yin, Qian Chen, Nan Xiang, Xudong Sun, Yunqiang Yang, Yongping Yang
Calcium-dependent protein kinases (CDPKs) as crucial sensors of calcium concentration changes play important roles in responding to abiotic and biotic stresses. In this study, 55 BrrCDPK genes, which were phylogenetically clustered into four subfamilies, were identified. Chromosome locations indicated that the CDPK family in turnip expanded by segmental duplication and genome rearrangement. Moreover, gene expression profiles showed that different BrrCDPKs were expressed in specific tissues or stages. Transcript levels of BrrCDPKs indicated that they were involved in abiotic and biotic stresses and that paralogs exhibited functional divergence...
November 17, 2017: Scientific Reports
Nehanjali Parmar, Ajay Kumar Thakur, Pardeep Kumar, P D Thakur, Sat Vrat Bhardwaj
Nine isolates of Turnip mosaic potyvirus (TuMV)-infecting radish collected from different regions of Northern India were characterized. All isolates except for New Delhi and Rajasthan isolates resulted positive for TuMV in double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). RNA was isolated from leaves of infected plants and used in reverse transcriptase-polymerase chain reaction (RT-PCR) with TuMV coat protein (CP) gene-specific primers. Viral amplicons of expected 1000 bp size were obtained, which were further subjected to cloning and sequencing...
December 2017: 3 Biotech
Anders Hafrén, Suayib Üstün, Anton Hochmuth, Steingrim Svenning, Terje Johansen, Daniel Hofius
Autophagy is a conserved intracellular degradation pathway and has emerged as a key mechanism of antiviral immunity in metazoans, including the selective elimination of viral components. In turn, some animal viruses are able to escape and modulate autophagy for enhanced pathogenicity. Whether host autophagic responses and viral countermeasures play similar roles in plant-virus interactions is not well understood. Here, we have identified selective autophagy as antiviral pathway during plant infection with turnip mosaic virus (TuMV), a positive-stranded RNA potyvirus...
November 13, 2017: Plant Physiology
Isabelle Jupin, Maya Ayach, Lucile Jomat, Sonia Fieulaine, Stéphane Bressanelli
The positive-strand RNA virus Turnip yellow mosaic virus (TYMV) encodes an ovarian tumor (OTU)-like protease/deubiquitinase (PRO/DUB) protein domain involved both in proteolytic processing of the viral polyprotein through its PRO activity, and in removal of ubiquitin chains from ubiquitylated substrates through its DUB activity. Here, the crystal structures of TYMV PRO/DUB mutants and molecular dynamics simulations reveal that an idiosyncratic mobile loop participates in reversibly constricting its unusual catalytic site by adopting "open", "intermediate" or "closed" conformations...
November 2017: PLoS Pathogens
Sylvain Poque, Hui-Wen Wu, Chung-Hao Huang, Hao-Wen Cheng, Wen-Chi Hu, Jun-Yi Yang, David Wang, Shyi-Dong Yeh
The viral infection process is a battle between host defense response and pathogen antagonizing action. Several studies have established a tight link between the viral RNA silencing suppressor (RSS) and the repression of salicylic acid (SA)-mediated defense responses, nonetheless host factors directly linking an RSS and the SA pathway remains unidentified. From yeast two-hybrid analysis, we identified an interaction between the potyviral RSS helper-component proteinase (HCPro) and SA-binding protein SABP3. Co-localization and bimolecular fluorescence complementation analyses validated the direct in vivo interaction between Turnip mosaic virus (TuMV) HCPro and the Arabidopsis homologue of SABP3, AtCA1...
November 1, 2017: Molecular Plant-microbe Interactions: MPMI
Nooshin Movahed, Camilo Patarroyo, Jiaqi Sun, Hojatollah Vali, Jean-Francois Laliberte, Huanquan Zheng
Plant viruses move from the initially infected cell to adjacent cells through plasmodesmata (PDs). To do so, viruses encode dedicated protein(s) that facilitate this process. How viral proteins act together to support intercellular movement of viruses is poorly defined. Here, we investigate action of CI (Cytoplasmic Inclusion) and P3N-PIPO (N-terminal half of P3 fused to Pretty Interesting Potyviridae ORF), two PD-localized potyviral proteins encoded by Turnip mosaic virus (TuMV) in the intercellular movement of the viral replication vesicles, using an infection-free intercellular vesicle movement assay...
October 31, 2017: Plant Physiology
Xiaoxiao Feng, Lixiang Pan, Chen Wang, Hongyan Zhang
A sensitive and selective method was developed and validated for the determination of pyrethrin residues in turnips (turnip leaves, turnip tubers, and the whole of plant) and cultivated soil using gas chromatography coupled with mass spectrometry (GC-MS). Six major components of pyrethrins (pyrethrin I and II, cinerin I and II, and jasmolin I and II) were separated and identified. The method involving solid-phase extraction (SPE) cleanup led to satisfactory average recoveries (88.1-104%) with limits of quantification (LOQs) of 0...
October 24, 2017: Environmental Science and Pollution Research International
Endang S Kusmintarsih, Rahma F Hayati, Oktaviani N Turnip, Benediktus Yohan, Suhestri Suryaningsih, Hery Pratiknyo, Dionisius Denis, R Tedjo Sasmono
BACKGROUND: Dengue is hyper-endemic in Indonesia. Purwokerto city in Central Java province is routinely ravaged by the disease. Despite the endemicity of dengue in this city, there is still no data on the virological aspects of dengue in the city. We conducted a molecular surveillance study of the circulating dengue viruses (DENV) in Purwokerto city to gain information on the virus origin, serotype and genotype distribution, and phylogenetic characteristics of DENV. METHODS: A cross-sectional dengue molecular surveillance study was conducted in Purwokerto...
October 19, 2017: Journal of Infection and Public Health
Alok Kumar, Manas Kumar Bag, Ranbir Singh, A Abdul Kader Jailani, Bikash Mandal, Anirban Roy
Crambe is an important crop grown worldwide for industrial oil and seed meal. Besides the fungal and bacterial diseases, the crop is reported to be infected by tobacco mosaic virus, beet western yellows virus and turnip mosaic virus under experimental condition. Till now, there was no report of natural infection of any begomovirus in this crop. In the present study, a leaf curl disease was observed in germplasm accessions of three species of Crambe (C. abyssinica, C. glabrata and C. hispanica). Based on the symptoms and presence of whitefly population in the field, begomovirus infection was suspected...
October 12, 2017: Virus Research
Kjersti Selstad Utaaker, Anil Kumar, Himanshu Joshi, Suman Chaudhary, Lucy J Robertson
Fresh produce has been recognized as a vehicle of infection for protozoan parasites, particularly Cryptosporidium, and, to a lesser extent, Giardia. For both parasites, outbreaks associated with fresh produce have been documented. Although documented outbreaks tend to be from industrialized countries, contamination of fresh produce with these parasites is a global issue. In developing countries, infections with these parasites are often endemic in the community, and basic infrastructure and hygiene measures may be inadequate, thus the likelihood of contamination of fresh produce with these parasites may be higher...
October 1, 2017: International Journal of Food Microbiology
Rebecca Klopsch, Katja Witzel, Andreas Börner, Monika Schreiner, Franziska S Hanschen
About 10% of the world's vegetable production is generated from Brassicaceae, wherein Brassica rapa is a dominating species. There is growing evidence that glucosinolates (GLSs), main plant secondary metabolites in Brassicales, play an important role in promoting human health. Natural genetic diversity of B. rapa can be explored for vegetable improvement. We analyzed leaves and tubers of 16 B. rapa turnips for their GLS composition by UHPLC-DAD and the corresponding hydrolysis products by GC-MS. Thirteen GLSs were identified, 8 aliphatic, 4 indolic and one aromatic...
October 2017: Food Research International
Johann M R Antoine, Leslie A Hoo Fung, Charles N Grant
Thirteen Jamaican-grown food crops - ackee (Blighia sapida), banana (Musa acuminate), cabbage (Brassica oleracea), carrot (Daucus carota), cassava (Manihot esculenta), coco (Xanthosoma sagittifolium), dasheen (Colocasia esculenta), Irish potato (Solanum tuberosum), pumpkin (Cucurbita pepo), sweet pepper (Capsicum annuum), sweet potato (Ipomoea batatas), tomato (Solanum lycopersicum) and turnip (Brassica rapa) - were analysed for aluminium, arsenic, cadmium and lead by atomic absorption spectrophotometry and instrumental neutron activation analysis...
2017: Toxicology Reports
Jiancan Du, Simin Hu, Qin Yu, Chongde Wang, Yunqiang Yang, Hang Sun, Yongping Yang, Xudong Sun
The teosinte branched1/cycloidea/proliferating cell factor (TCP) gene family is a plant-specific transcription factor that participates in the control of plant development by regulating cell proliferation. However, no report is currently available about this gene family in turnips (Brassica rapa ssp. rapa). In this study, a genome-wide analysis of TCP genes was performed in turnips. Thirty-nine TCP genes in turnip genome were identified and distributed on 10 chromosomes. Phylogenetic analysis clearly showed that the family was classified as two clades: class I and class II...
2017: Frontiers in Plant Science
Justyna Boniecka, Justyna Prusińska, Grażyna B Dąbrowska, Anna Goc
Plant RSH proteins are able to synthetize and/or hydrolyze unusual nucleotides called (p)ppGpp or alarmones. These molecules regulate nuclear and chloroplast transcription, chloroplast translation and plant development and stress response. Homologs of bacterial RelA/SpoT proteins, designated RSH, and products of their activity, (p)ppGpp-guanosine tetra-and pentaphosphates, have been found in algae and higher plants. (p)ppGpp were first identified in bacteria as the effectors of the stringent response, a mechanism that orchestrates pleiotropic adaptations to nutritional deprivation and various stress conditions...
November 2017: Planta
Sang-Ho Park, Fangfang Li, Justin Renaud, Wentao Shen, Yinzi Li, Lihua Guo, Hongguang Cui, Mark Sumarah, Aiming Wang
Plasmodesmata (PD), unique to the plant kingdom, are structurally complex microchannels that cross the cell wall to establish symplastic communication between neighboring cells. Viral intercellular movement occurs through PD. To better understand the involvement of PD in viral infection, we conducted a quantitative proteomic study on the PD-enriched fraction from Nicotiana benthamiana leaves in response to infection by Turnip mosaic virus (TuMV). We report the identification of a total of 1070 PD protein candidates, of which 100 (≥ 2-fold increase) and 48 (≥ 2-fold reduction) are significantly differentially accumulated in the PD-enriched fraction, when compared with protein levels in the corresponding healthy control...
September 23, 2017: Plant Journal: for Cell and Molecular Biology
Liv de Vries, Keram Pfeiffer, Björn Trebels, Andrea K Adden, Ken Green, Eric Warrant, Stanley Heinze
Brain structure and function are tightly correlated across all animals. While these relations are ultimately manifestations of differently wired neurons, many changes in neural circuit architecture lead to larger-scale alterations visible already at the level of brain regions. Locating such differences has served as a beacon for identifying brain areas that are strongly associated with the ecological needs of a species-thus guiding the way towards more detailed investigations of how brains underlie species-specific behaviors...
2017: Frontiers in Behavioral Neuroscience
Robin, Rohit Arora, Saroj Arora, Adarsh Pal Vig
In the present study reverse-phase UHPLC-PDA technique was developed at 60°C for simultaneous quantification of allyl, 3-butenyl, 4-(methylthio)butyl, benzyl and phenethyl isothiocyanates. The validation parameter showed a very good linearity, with a correlation coefficient of 1.00 for all detected standard analytes. Also, high precision and accuracy were observed with lowest obtained values of 1.39% and 99.1%, respectively. Different varieties of three plants, viz. Brassica rapa var. rapa L., Raphanus sativus L...
January 15, 2018: Food Chemistry
Xiangdong Li, Tiansheng Zhu, Xiao Yin, Chengling Zhang, Jia Chen, Yanping Tian, Jinliang Liu
BACKGROUND: Turnip mosaic virus (TuMV) is one of the most widespread and economically important virus infecting both crop and ornamental species of the family Brassicaceae. TuMV isolates can be classified to five phylogenetic lineages, basal-B, basal-BR, Asian-BR, world-B and Orchis. RESULTS: To understand the genetic structure of TuMV from radish in China, the 3'-terminal genome of 90 TuMV isolates were determined and analyzed with other available Chinese isolates...
August 29, 2017: Virology Journal
Harri Juhani Saarinen, Chaiyasit Sittiwet, Piia Simonen, Markku J Nissinen, Ulf-Håkan Stenman, Helena Gylling, Ari Palomäki
We have earlier reported the reduction of total cholesterol, low-density lipoprotein (LDL) cholesterol and oxidized LDL caused by short-term modification of diet with cold-pressed turnip rapeseed oil (CPTRO) instead of butter. The aim of this supplementary study was to determine whether the beneficial effects resulted from altered cholesterol metabolism during the intervention.Thirty-seven men with metabolic syndrome (MetS) completed an open, randomized and balanced crossover study. Subjects' usual diet was supplemented with either 37...
August 11, 2017: Journal of Investigative Medicine: the Official Publication of the American Federation for Clinical Research
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