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https://www.readbyqxmd.com/read/29670641/genome-wide-identification-and-expression-analysis-of-the-cation-diffusion-facilitator-gene-family-in-turnip-under-diverse-metal-ion-stresses
#1
Xiong Li, Yuansheng Wu, Boqun Li, Wenqi He, Yonghong Yang, Yongping Yang
The cation diffusion facilitator (CDF) family is one of the gene families involved in metal ion uptake and transport in plants, but the understanding of the definite roles and mechanisms of most CDF genes remain limited. In the present study, we identified 18 candidate CDF genes from the turnip genome and named them BrrMTP1.1 - BrrMTP12 . Then, we performed a comparative genomic analysis on the phylogenetic relationships, gene structures and chromosome distributions, conserved domains, and motifs of turnip CDFs...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29655730/nmr-and-gc-ms-based-metabolomic-characterization-of-sunki-an-unsalted-fermented-pickle-of-turnip-leaves
#2
Satoru Tomita, Toshihide Nakamura, Sanae Okada
This study revealed the compositional characteristics of sunki, a traditional, unsalted, lactic acid-fermented pickle produced using turnip leaf in Kiso district, Japan. Comprehensive compositional analysis by two metabolomic approaches based on NMR and solid-phase microextraction-GC/MS methods was used to determine its chemical composition by annotating 54 water-soluble and 62 volatile compounds. Principal component analysis showed that samples had different compositions, depending on the agricultural processing factory and production year...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29620847/trace-detecting-opcwas-in-wastewater-and-plant-by-a-luminescent-uio-67-hf-and-evaluate-bioaccumulation-of-opcwas
#3
Xiao Lian, Bing Yan
OPCWAs are a group of organic pollutants characterized by high toxicity and chemical stability, and they are very difficult to be degraded. The trace quality of OPCWAs in water and food will cause great harm to the human body. Therefore, the detection of OPCWAs is a difficult challenge and research hotspots over the world. In this work, a Hf-based luminescent metal-organic framework (Eu@1) is prepared, and the reactivity of Hf12 result a MPA induced luminescence quenching, the charge transfer from MPA to Hf(Ⅳ) and generated exciplex is responsible for this quenching effect...
April 5, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29618450/evaluation-of-phytochemical-and-in-vitro-antioxidant-activity-of-water-and-acetone-extracts-of-selected-vegetables
#4
Faria Nisar, Muhammad Aslam Mirza, Riffat Naseem Malik, Muhammad Aziz Ch, Kauser Yasmeen
The aim of this study was to determine antioxidant activity of Brassica oleracea (Cabbage), Brassica rapa (Turnip) and Raphanus sativus (Radish). All of the three selected vegetables were ultrasonically extracted in water and acetone. The antioxidant activity was determined by DPPH and H2O2. Highest phenolic, flavonoid and flavonol contents were found among water extract of Raphanus sativus, Brassica oleracea and Brassica rapa respectively. Highest %age of DPPH inhibition was exhibited by water extract of Brassica rapa and highest OH◦ radical scavenging activity was shown by water extract of Raphanus sativus...
March 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29603105/metal-accumulation-in-raphanus-sativus-and-brassica-rapa-an-assessment-of-potential-health-risk-for-inhabitants-in-punjab-pakistan
#5
Kafeel Ahmad, Asma Ashfaq, Zafar Iqbal Khan, Humayun Bashir, Muhammad Sohail, Naunain Mehmood, Yunus Dogan
Pakistan is an agricultural country and due to the shortage of clean water, most of the irrigated area (32,500 ha) of Pakistan was supplied with wastewater (0.876 × 109 m3 /year). Concentrations of heavy metals in radish (Raphanus sativus) and turnip (Brassica rapa) taken from vegetable fields in Sargodha, Pakistan, were measured. Untreated wastewater was used persistently for a long time to irrigate these vegetable fields. A control site was selected that had a history of fresh groundwater irrigation...
March 30, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29593696/transmission-of-turnip-yellows-virus-by-myzus-persicae-is-reduced-by-feeding-aphids-on-double-stranded-rna-targeting-the-ephrin-receptor-protein
#6
Michaël Mulot, Baptiste Monsion, Sylvaine Boissinot, Maryam Rastegar, Sophie Meyer, Nicole Bochet, Véronique Brault
Aphid-transmitted plant viruses are a threat for major crops causing massive economic loss worldwide. Members in the Luteoviridae family are transmitted by aphids in a circulative and non-replicative mode. Virions are acquired by aphids when ingesting sap from infected plants and are transported through the gut and the accessory salivary gland (ASG) cells by a transcytosis mechanism relying on virus-specific receptors largely unknown. Once released into the salivary canal, virions are inoculated to plants, together with saliva, during a subsequent feeding...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29593293/beclin1-restricts-rna-virus-infection-in-plants-through-suppression-and-degradation-of-the-viral-polymerase
#7
Fangfang Li, Changwei Zhang, Yinzi Li, Guanwei Wu, Xilin Hou, Xueping Zhou, Aiming Wang
Autophagy emerges as an essential immunity defense against intracellular pathogens. Here we report that turnip mosaic virus (TuMV) infection activates autophagy in plants and that Beclin1 (ATG6), a core component of autophagy, inhibits virus replication. Beclin1 interacts with NIb, the RNA-dependent RNA polymerase (RdRp) of TuMV, via the highly conserved GDD motif and the interaction complex is targeted for autophagic degradation likely through the adaptor protein ATG8a. Beclin1-mediated NIb degradation is inhibited by autophagy inhibitors...
March 28, 2018: Nature Communications
https://www.readbyqxmd.com/read/29587648/identification-of-cold-stress-responsive-micrornas-in-two-winter-turnip-rape-brassica-rapa-l-by-high-throughput-sequencing
#8
Xiucun Zeng, Yaozhao Xu, Jinjin Jiang, Fenqin Zhang, Li Ma, Dewei Wu, Youping Wang, Wancang Sun
BACKGROUND: Low temperature is a major abiotic stress affecting the production of rapeseed in China by impeding plant growth and development. A comprehensive knowledge of small-RNA expression pattern in Brassica rapa under cold stress could improve our knowledge of microRNA-mediated stress responses. RESULTS: A total of 353 cold-responsive miRNAs, 84 putative novel and 269 conserved miRNAs, were identified from the leaves and roots of two winter turnip rape varieties 'Longyou 7' (cold-tolerant) and 'Tianyou 4' (cold-sensitive), which were stressed under - 4 °C for 8 h...
March 27, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29566219/animal-performance-and-environmental-efficiency-of-cool-and-warm-season-annual-grazing-systems
#9
S Leanne Dillard, Dennis W Hancock, Deidre D Harmon, M Kimberly Mullenix, Paul A Beck, Kathy J Soder
Annual forage crops can provide short-term grazing between crop rotations or can be interseeded into perennial pastures to increase forage quality and productivity. They also provide an opportunity to increase the economic and environmental sustainability of grazing systems. Cool-season annual forage crops provide high-quality, abundant forage biomass when forage availability from perennial forage species is lacking, reducing the need for stored feeds during the winter months. For example, ADG of 1.5 kg have been reported using small grains alone and in mixtures with annual ryegrass (Lolium multiflorum Lam...
March 16, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29552557/effects-of-foliar-selenite-on-the-nutrient-components-of-turnip-brassica-rapa-var-rapa-linn
#10
Xiong Li, Boqun Li, Yongping Yang
We administered foliar applications of 50, 100, and 200 mg L-1 selenium (Se, selenite) on turnip ( Brassica rapa var. rapa Linn.) and detected the changes in the main nutrient components in fleshy roots. Results showed that the foliar application of Se (IV) significantly increased the Se content in turnip, and Se (IV) positively affected the uptake of several mineral elements, including magnesium, phosphorus, iron, zinc, manganese, and copper. Se (IV) treatments also improved the synthesis of protein and multiple amino acids instead of crude fat and total carbohydrate in turnip, indicating that the foliar application of Se (IV) could enhance Se biofortification in turnip and promote its nutritional value...
2018: Frontiers in Chemistry
https://www.readbyqxmd.com/read/29538326/tomato-spotted-wilt-virus-nss-protein-supports-infection-and-systemic-movement-of-a-potyvirus-and-is-a-symptom-determinant
#11
Hernan Garcia-Ruiz, Sergio M Gabriel Peralta, Patricia A Harte-Maxwell
Plant viruses are inducers and targets of antiviral RNA silencing. To condition susceptibility, most plant viruses encode silencing suppressor proteins that interfere with antiviral RNA silencing. The NSs protein is an RNA silencing suppressor in orthotospoviruses, such as the tomato spotted wilt virus (TSWV). The mechanism of RNA silencing suppression by NSs and its role in virus infection and movement are poorly understood. Here, we cloned and tagged TSWV NSs and expressed it from a GFP-tagged turnip mosaic virus (TuMV-GFP) carrying either a wild-type or suppressor-deficient (AS9) helper component proteinase (HC-Pro)...
March 14, 2018: Viruses
https://www.readbyqxmd.com/read/29517848/the-apparent-non-host-resistance-of-ethiopian-mustard-to-a-radish-infecting-strain-of-turnip-mosaic-virus-is-largely-determined-by-the-c-terminal-region-of-the-p3-viral-protein
#12
Papaiah Sardaru, Laura Sinausía, Silvia López-González, Jelena Zindovic, Flora Sánchez, Fernando Ponz
Two different isolates of Turnip mosaic virus (UK 1 and JPN 1) belonging to different virus strains were tested on three different Brassica species, namely turnip (Brassica rapa L.), Indian mustard (Brassica juncea L.), and Ethiopian mustard (Brassica carinata A. Braun). Whereas all three hosts were readily infected by isolate UK 1, isolate JPN 1 was able to establish a visible systemic infection only in the first two. Ethiopian mustard plants showed no local or systemic symptoms, and no virus antigens could be detected by ELISA...
March 8, 2018: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29513740/cop1-a-negative-regulator-of-photomorphogenesis-positively-regulates-plant-disease-resistance-via-double-stranded-rna-binding-proteins
#13
Gah-Hyun Lim, Timothy Hoey, Shifeng Zhu, Marion Clavel, Keshun Yu, Duroy Navarre, Aardra Kachroo, Jean-Marc Deragon, Pradeep Kachroo
The E3 ubiquitin ligase COP1 (Constitutive Photomorphogenesis 1) is a well known component of the light-mediated plant development that acts as a repressor of photomorphogenesis. Here we show that COP1 positively regulates defense against turnip crinkle virus (TCV) and avrRPM1 bacteria by contributing to stability of resistance (R) protein HRT and RPM1, respectively. HRT and RPM1 levels and thereby pathogen resistance is significantly reduced in the cop1 mutant background. Notably, the levels of at least two double-stranded RNA binding (DRB) proteins DRB1 and DRB4 are reduced in the cop1 mutant background suggesting that COP1 affects HRT stability via its effect on the DRB proteins...
March 7, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29492896/shannon-entropy-to-evaluate-substitution-rate-variation-among-viral-nucleotide-positions-in-datasets-of-viral-sirnas
#14
Aysan Ghasemzadeh, Marta Małgorzata Ter Haar, Masoud Shams-Bakhsh, Walter Pirovano, Vitantonio Pantaleo
Next-generation sequencing has opened the door to the reconstruction of viral populations and examination of the composition of mutant spectra in infected cells, tissues, and host organisms. In this chapter we present details on the use of the Shannon entropy method to estimate the site-specific nucleotide relative variability of turnip crinkle virus, a positive (+) stranded RNA plant virus, in a large dataset of short RNAs of Cicer arietinum L., a natural reservoir of the virus. We propose this method as a viral metagenomics tool to provide a more detailed description of the viral quasispecies in infected plant tissue...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29471524/enteric-methane-production-and-ruminal-fermentation-of-forage-brassica-diets-fed-in-continuous-culture
#15
S Leanne Dillard, Ana I Roca-Fernández, Melissa D Rubano, Kyle R Elkin, Kathy J Soder
The aim of the current study was to determine nutrient digestibility, VFA production, N metabolism, and CH4 production of canola (Brassica napus L.), rapeseed (B. napus L.), turnip (B. rapa L.), and annual ryegrass (Lolium multiflorum Lam.) fed with orchardgrass (Dactylis glomerata L.) in continuous culture. Diets were randomly assigned to fermentors in a 4 × 4 Latin square design using 7 d for adaptation and 3 d for collection. Diets were: 1) 50% orchardgrass + 50% annual ryegrass (ARG); 2) 50% orchardgrass + 50% canola (CAN); 3) 50% orchardgrass + 50% rapeseed (RAP); and 4) 50% orchardgrass + 50% turnip (TUR)...
February 17, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29460191/multivariate-analysis-and-health-risk-assessment-of-heavy-metal-contents-in-foodstuffs-of-durban-south-africa
#16
Sanjay Kumar Gupta, Faiz Ahmed Ansari, Mahmoud Nasr, Mayuri Chabukdhara, Faizal Bux
This study presented a comprehensive analysis of heavy metal contents in foodstuffs and the associated health risk for the residential population in Durban, South Africa. The concentrations of elements in fruits and vegetables, respectively, were (in mg kg-1 dry weight) Cu, 0.52-1.47 and 0.27-2.25; Zn, 0.30-3.05 and 0.56-6.24, Fe, 1.70-22.60 and 0.73-44.90; Mn, 0.37-28.50 and 0.67-13.70; Cr, 0.47-1.47 and 0.37-3.06; Ni 0.03-1.14 and 0.11-2.5;, and Pb, 1.52-3.45 and 1.57-4.52. Multivariate analysis revealed that pineapple in fruits and turnip and carrot in vegetables contained remarkable components of trace metals...
February 19, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29422912/a-new-mechanistic-model-for-viral-cross-protection-and-superinfection-exclusion
#17
REVIEW
Xiao-Feng Zhang, Shaoyan Zhang, Qin Guo, Rong Sun, Taiyun Wei, Feng Qu
Plants pre-infected with a mild variant of a virus frequently become protected against more severe variants of the same virus through the cross protection phenomenon first discovered in 1929. Despite its widespread use in managing important plant virus diseases, the mechanism of cross protection remains poorly understood. Recent investigations in our labs, by analyzing the whole-plant dynamics of a turnip crinkle virus (TCV) population, coupled with cell biological interrogation of individual TCV variants, revealed possible novel mechanisms for cross protection and the closely related process of superinfection exclusion (SIE)...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29411542/prevalence-of-psychotic-like-experiences-and-their-correlations-with-internalizing-problems-a-study-of-early-adolescents-in-rural-area-in-karawang-indonesia
#18
Lerivia Maharani, Sherly Saragih Turnip
INTRODUCTION: Adolescents who live in rural areas have been found to experience more mental health problems than those who live in urban areas. In Indonesia, adolescents who live in rural areas have limited facilities to access mental health services. On the other hand, there is still a strong belief in the rural communities that mental health problems are the consequence of demonic possession, having committed a sin, lack of faith, etc. Rural communities tend to seek traditional remedies such as advice from traditional healers or witchdoctors...
February 6, 2018: Asia-Pacific Psychiatry: Official Journal of the Pacific Rim College of Psychiatrists
https://www.readbyqxmd.com/read/29322007/co-administration-effects-of-aqueous-extract-of-turnip-leaf-and-metformin-in-diabetic-rats
#19
Moammadmehdi Hassanzadeh-Taheri, Mohammad Hassanpour-Fard, Mohammadreza Doostabadi, Hesam Moodi, Khadijeh Vazifeshenas-Darmiyan, Mehran Hosseini
Background: There is a variety of experimentally proven medicinal plants having antidiabetic properties but data on herb-drug interaction are very limited. Earlier studies indicated that aqueous extract of turnip leaf (AETL) has hypoglycemic potential in diabetic animals. The present study was conducted to evaluate co-administration effects of AETL and metformin, a commonly used antidiabetic drug, in diabetic rats. Methods: Metformin at the two different doses (50,100 mg/kg) and AETL at the dose of 400 mg/kg (separately or concurrent with metformin) were orally given to streptozotocin-induced diabetic rats for 4 weeks daily...
January 2018: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/29305079/combinatorial-effect-of-photorhabdus-luminescens-tt01-and-bacillus-thuringiensis-vip3aa16-toxin-against-agrotis-segetum
#20
Wafa Jallouli, Hanen Boukedi, Sameh Sellami, Fakher Frikha, Lobna Abdelkefi-Mesrati, Slim Tounsi
The entomopathogenic Photorhabdus luminescens TT01 is a promoting bacterium that controls effectively many insect pests. Indeed, it exhibited a mortality rate of 32.36% against the first instar larvae of the turnip moth Agrotis segetum, when it was used at a concentration of 5 × 107  cells/ml but no toxicity against the second instar larvae in the same condition. P. luminescens TT01 oral toxicity is associated to septicaemia since cells fraction exhibited the highest mortality rate of 34%. In order to enhance P...
February 2018: Toxicon: Official Journal of the International Society on Toxinology
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