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https://www.readbyqxmd.com/read/28672820/improved-method-for-reliable-hmw-gs-identification-by-rp-hplc-and-sds-page-in-common-wheat-cultivars
#1
You-Ran Jang, Hye-Rang Beom, Susan B Altenbach, Min-Ki Lee, Sun-Hyung Lim, Jong-Yeol Lee
The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 16 standard wheat cultivars with known HMW-GS compositions. Subunits that were not identified unambiguously by RP-HPLC were distinguished by SDS-PAGE or inferred from association with linked subunits...
June 24, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28443107/virus-based-microrna-silencing-and-overexpressing-in-common-wheat-triticum-aestivum-l
#2
Chao Jian, Ran Han, Qing Chi, Shijuan Wang, Meng Ma, Xiangli Liu, Huixian Zhao
MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that arise from large RNA precursors with a stem-loop structure and play important roles in plant development and responses to environmental stress. Although a hundred and nineteen wheat miRNAs have been identified and registered in the miRBase (Release 21.0, June, 2014; http://www.mirbase.org), the functional characterization of these miRNAs in wheat growth and development is lagging due to lack of effective techniques to investigate endogenous miRNA functions in wheat...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28335373/dietary-patterns-and-insomnia-symptoms-in-chinese-adults-the-china-kadoorie-biobank
#3
Canqing Yu, Zumin Shi, Jun Lv, Yu Guo, Zheng Bian, Huaidong Du, Yiping Chen, Ran Tao, Ying Huang, Junshi Chen, Zhengming Chen, Liming Li
Limited attention has been paid to the effect of dietary patterns on sleep problems. In the present study, we analyzed the cross-sectional data of 481,242 adults aged 30-79 years from the China Kadoorie Biobank. A laptop-based questionnaire was administered to collect information on food intakes and insomnia symptoms. Logistic regression was used to estimate the odds ratios of each insomnia symptom according to quartiles of each dietary pattern, with adjustment for potential confounders. Two major dietary patterns were derived by factor analysis...
March 4, 2017: Nutrients
https://www.readbyqxmd.com/read/28285205/soil-heavy-metal-contamination-and-health-risks-associated-with-artisanal-gold-mining-in-tongguan-shaanxi-china
#4
Ran Xiao, Shuang Wang, Ronghua Li, Jim J Wang, Zengqiang Zhang
Soil contamination with heavy metals due to mining activities poses risks to ecological safety and human well-being. Limited studies have investigated heavy metal pollution due to artisanal mining. The present study focused on soil contamination and the health risk in villages in China with historical artisanal mining activities. Heavy metal levels in soils, tailings, cereal and vegetable crops were analyzed and health risk assessed. Additionally, a botany investigation was conducted to identify potential plants for further phytoremediation...
July 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28244994/precise-base-editing-in-rice-wheat-and-maize-with-a-cas9-cytidine-deaminase-fusion
#5
Yuan Zong, Yanpeng Wang, Chao Li, Rui Zhang, Kunling Chen, Yidong Ran, Jin-Long Qiu, Daowen Wang, Caixia Gao
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.
May 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28197689/influence-of-straw-incorporation-with-and-without-straw-decomposer-on-soil-bacterial-community-structure-and-function-in-a-rice-wheat-cropping-system
#6
Jun Zhao, Tian Ni, Weibing Xun, Xiaolei Huang, Qiwei Huang, Wei Ran, Biao Shen, Ruifu Zhang, Qirong Shen
To study the influence of straw incorporation with and without straw decomposer on bacterial community structure and biological traits, a 3-year field experiments, including four treatments: control without fertilizer (CK), chemical fertilizer (NPK), chemical fertilizer plus 7500 kg ha(-1) straw incorporation (NPKS), and chemical fertilizer plus 7500 kg ha(-1) straw incorporation and 300 kg ha(-1) straw decomposer (NPKSD), were performed in a rice-wheat cropping system in Changshu (CS) and Jintan (JT) city, respectively...
February 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28159269/characterization-of-lentinus-edodes-%C3%AE-glucan-influencing-the-in-vitro-starch-digestibility-of-wheat-starch-gel
#7
Haining Zhuang, Zhongqiu Chen, Tao Feng, Yan Yang, Jingsong Zhang, Guodong Liu, Zhaofeng Li, Ran Ye
Lentinus edodes β-glucan (abbreviated LEBG) was prepared from fruiting bodies of Lentinus edodes. The average molecular weight (Mw) and polydispersity index (Mw/Mn) of LEBG were measured to be 1.868×10(6)g/mol and 1.007, respectively. In addition, the monosaccharide composition of LEBG was composed of arabinose, galactose, glucose, xylose, mannose with a molar ratio of 5:11:18:644:16. After adding LEBG, both G' and G″ of starch gel increased. This is mainly because the connecting points between the molecular chains of LEBG and starch formed so that gel network structures were enhanced...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28098143/efficient-dna-free-genome-editing-of-bread-wheat-using-crispr-cas9-ribonucleoprotein-complexes
#8
Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao
Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop breeding. The avoidance of transgene integration and reduction of off-target mutations are the most important targets for optimization. Here, we describe an efficient genome editing method for bread wheat using CRISPR/Cas9 ribonucleoproteins (RNPs). Starting from RNP preparation, the whole protocol takes only seven to nine weeks, with four to five independent mutants produced from 100 immature wheat embryos. Deep sequencing reveals that the chance of off-target mutations in wheat cells is much lower in RNP mediated genome editing than in editing with CRISPR/Cas9 DNA...
January 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/27987446/exploring-the-interactions-and-binding-sites-between-cd-and-functional-groups-in-soil-using-two-dimensional-correlation-spectroscopy-and-synchrotron-radiation-based-spectromicroscopies
#9
Fusheng Sun, Matthew L Polizzotto, Dongxing Guan, Jun Wu, Qirong Shen, Wei Ran, Boren Wang, Guanghui Yu
Understanding how heavy metals bind and interact in soils is essential for predicting their distributions, reactions and fates in the environment. Here we propose a novel strategy, i.e., combining two-dimensional correlation spectroscopy (2D COS) and synchrotron radiation based spectromicroscopies, for identifying heavy metal binding to functional groups in soils. The results showed that although long-term (23 yrs) organic fertilization treatment caused the accumulation of Cd (over 3 times) in soils when compared to no fertilization and chemical fertilization treatments, it significantly (p<0...
December 12, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27734916/characterization-of-a-ran-gene-from-puccinia-striiformis-f-sp-tritici-involved-in-fungal-growth-and-anti-cell-death
#10
Yulin Cheng, Juanni Yao, Yanru Zhang, Shumin Li, Zhensheng Kang
Ran, an important family of small GTP-binding proteins, has been shown to regulate a variety of important cellular processes in many eukaryotes. However, little is known about Ran function in pathogenic fungi. In this study, we report the identification and functional analysis of a Ran gene (designated PsRan) from Puccinia striiformis f. sp. tritici (Pst), an important fungal pathogen affecting wheat production worldwide. The PsRan protein contains all conserved domains of Ran GTPases and shares more than 70% identity with Ran proteins from other organisms, indicating that Ran proteins are conserved in different organisms...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27582405/a-multicenter-retrospective-case-study-of-anaphylaxis-triggers-by-age-in-korean-children
#11
So Yeon Lee, Kangmo Ahn, Jihyun Kim, Gwang Cheon Jang, Taek Ki Min, Hyeon Jong Yang, Bok Yang Pyun, Ji Won Kwon, Myung Hyun Sohn, Kyung Won Kim, Kyu Earn Kim, Jinho Yu, Soo Jong Hong, Jung Hyun Kwon, Sung Won Kim, Tae Won Song, Woo Kyung Kim, Hyung Young Kim, You Hoon Jeon, Yong Ju Lee, Hae Ran Lee, Hye Young Kim, Youngmin Ahn, Hye Yung Yum, Dong In Suh, Hyun Hee Kim, Jin Tack Kim, Jeong Hee Kim, Yong Mean Park, Sooyoung Lee
PURPOSE: Although anaphylaxis is recognized as an important, life-threatening condition, data are limited regarding its triggers in different age groups. We aimed to identify anaphylaxis triggers by age in Korean children. METHODS: We performed a retrospective review of medical records for children diagnosed with anaphylaxis between 2009 and 2013 in 23 secondary or tertiary hospitals in South Korea. RESULTS: A total of 991 cases (mean age=5...
November 2016: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27372141/volatile-organic-compound-emissions-from-straw-amended-agricultural-soils-and-their-relations-to-bacterial-communities-a-laboratory-study
#12
Juan Zhao, Zhe Wang, Ting Wu, Xinming Wang, Wanhong Dai, Yujie Zhang, Ran Wang, Yonggan Zhang, Chengfei Shi
A laboratory study was conducted to investigate volatile organic compound (VOC) emissions from agricultural soil amended with wheat straw and their associations with bacterial communities for a period of 66days under non-flooded and flooded conditions. The results indicated that ethene, propene, ethanol, i-propanol, 2-butanol, acetaldehyde, acetone, 2-butanone, 2-pentanone and acetophenone were the 10 most abundant VOCs, making up over 90% of the total VOCs released under the two water conditions. The mean emission of total VOCs from the amended soils under the non-flooded condition (5924ng C/(kg·hr)) was significantly higher than that under the flooded condition (2211ng C/(kg·hr))...
July 2016: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/27183793/-the-inheritance-of-endosperm-storage-proteins-by-the-line-of-the-saratovskaya-29-cultivar-of-common-wheat-from-its-parental-forms
#13
L V Obukhova, V K Shumny
We ran a comparative analysis of storage proteins (gliadins, high- (HMW) and low-molecular-weight (LMW) glutenins, puroindolines, and exogenous α-amylase pest inhibitors) in the Saratovskaya 29 cultivar line from the collection of a genetic engineering laboratory, its parental forms (Albidum 24 and Lyutescens 55/11), and distant ancestors (Poltavka, Selivanovskiy Rusak, Sarroza, and tetraploid Beloturka). It was confirmed that the allelic states of storage proteins in the Gli-1, Gli-2 and Glu-1 loci originate from ancestral forms from the collection of the Vavilov Institute of Plant Industry...
January 2016: Genetika
https://www.readbyqxmd.com/read/26836300/molecular-and-cytogenetic-characterization-of-a-powdery-mildew-resistant-wheat-aegilops-mutica-partial-amphiploid-and-addition-line
#14
Cheng Liu, Guang-Rong Li, Wen-Ping Gong, Gen-Ying Li, Ran Han, Hao-Sheng Li, Jian-Min Song, Ai-Feng Liu, Xin-You Cao, Xiu-Sheng Chu, Zu-Jun Yang, Cheng-Yan Huang, Zhen-Dong Zhao, Jian-Jun Liu
Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA)8 as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of Ae. mutica chromosomes, with both wheat 7B chromosomes missing...
2015: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/26657387/heavy-metal-contents-distribution-and-prediction-in-a-regional-soil-wheat-system
#15
Jing Ran, Dejian Wang, Can Wang, Gang Zhang, Hailin Zhang
The entry of heavy metals into the food chain is of concern for potential health risks. To investigate the spatial relationships of heavy metals in a regional soil-wheat system, 99 pairs of surface soil (0-15 cm) and wheat grain samples were collected from Changshu, China, a typical county in the Yangtze Delta region. Both soil and wheat grain samples were analyzed for total Cd, Cu, Ni, Pb, and Zn. DTPA-extractable metals and major physico-chemical properties were also determined for soil samples. Moderate accumulation of heavy metals was found in soils and wheat grains, especially Cd...
February 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/26329893/enhancing-the-thermostability-of-feruloyl-esterase-estf27-by-directed-evolution-and-the-underlying-structural-basis
#16
Li-chuang Cao, Ran Chen, Wei Xie, Yu-huan Liu
To improve the thermostability of EstF27, two rounds of random mutagenesis were performed. A thermostable mutant, M6, with six amino acid substitutions was obtained. The half-life of M6 at 55 °C is 1680 h, while that of EstF27 is 0.5 h. The Kcat/Km value of M6 is 1.9-fold higher than that of EstF27. The concentrations of ferulic acid released from destarched wheat bran by EstF27 and M6 at their respective optimal temperatures were 223.2 ± 6.8 and 464.8 ± 11.9 μM, respectively. To further understand the structural basis of the enhanced thermostability, the crystal structure of M6 is determined at 2...
September 23, 2015: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/26295045/a-comparison-of-food-supply-from-1984-to-2009-and-degree-of-dietary-westernization-in-taiwan-with-asian-countries-and-world-continents
#17
COMPARATIVE STUDY
Cheau-Jane Peng, Cheng-Yao Lin, How-Ran Guo
OBJECTIVE: To compare quality, quantity, and trends of food supply from 1984 to 2009 and degree of food westernization in Taiwan with Asian countries and world continents by using food balance data. METHODS: We compiled data from food balance sheets of Taiwan and Food and Agriculture Organization, including five continents and three most populated countries each in Eastern, Southern, and Southeastern Asia over the period 1984-2009. Quantity of food supply per capita was referenced to Taiwan food guides...
2015: BioMed Research International
https://www.readbyqxmd.com/read/25854602/biochemical-characterization-of-caffeoyl-coenzyme-a-3-o-methyltransferase-from-wheat
#18
Qing-Hu Ma, Hao-Ran Luo
TaCCoAOMT1 is located in wheat chromosome 7A and highly expressed in stem and root. It is important for lignin biosynthesis, and associated with stem maturity but not lodging resistance. Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are one important class of enzymes to carry out the transfer of the methyl group from S-adenosylmethionine to the hydroxyl group, and play important roles in lignin and flavonoids biosynthesis. In the present study, sequences for CCoAOMT from the wheat genome were analyzed...
July 2015: Planta
https://www.readbyqxmd.com/read/25766807/blasting-extrusion-processing-the-increase-of-soluble-dietary-fiber-content-and-extraction-of-soluble-fiber-polysaccharides-from-wheat-bran
#19
Xiaoguang Yan, Ran Ye, Ye Chen
In this study, soluble dietary fiber (SDF) content of wheat bran was significantly increased from 9.82 ± 0.16 (w/w, %) to 16.72 ± 0.28 (w/w, %) by a novel blasting extrusion processing with enhanced water retention capacity and the swelling capacity. In addition, a water-soluble polysaccharide (WBP) was isolated and extracted from extruded SDF. WBP was successfully purified from SDF by column chromatography systems with the average molecular weight (Mw) of 4.7 × 10(4)Da, containing arabinose, xylose, glucose, and galactose...
August 1, 2015: Food Chemistry
https://www.readbyqxmd.com/read/25158525/-soil-respiration-and-carbon-balance-in-wheat-field-under-conservation-tillage
#20
Sai Zhang, Long-Chang Wang, Zhao-Cun Huang, Hui-Juan Jia, Chun-Yan Ran
In order to study the characteristics of carbon sources and sinks in the winter wheat farmland ecosystem in southwest hilly region of China, the LI6400-09 respiratory chamber was adopted in the experiment conducted in the experimental field in Southwest University in Chongqing. The soil respiration and plant growth dynamics were analyzed during the growth period of wheat in the triple intercropping system of wheat-maize-soybean. Four treatments including T (traditional tillage), R (ridge tillage), TS (traditional tillage + straw mulching), and RS (ridge tillage + straw mulching) were designed...
June 2014: Huan Jing Ke Xue= Huanjing Kexue
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