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https://www.readbyqxmd.com/read/27734916/characterization-of-a-ran-gene-from-puccinia-striiformis-f-sp-tritici-involved-in-fungal-growth-and-anti-cell-death
#1
Yulin Cheng, Juanni Yao, Yanru Zhang, Shumin Li, Zhensheng Kang
Ran, an important family of small GTP-binding proteins, has been shown to regulate a variety of important cellular processes in many eukaryotes. However, little is known about Ran function in pathogenic fungi. In this study, we report the identification and functional analysis of a Ran gene (designated PsRan) from Puccinia striiformis f. sp. tritici (Pst), an important fungal pathogen affecting wheat production worldwide. The PsRan protein contains all conserved domains of Ran GTPases and shares more than 70% identity with Ran proteins from other organisms, indicating that Ran proteins are conserved in different organisms...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27582405/a-multicenter-retrospective-case-study-of-anaphylaxis-triggers-by-age-in-korean-children
#2
So Yeon Lee, Kangmo Ahn, Jihyun Kim, Gwang Cheon Jang, Taek Ki Min, Hyeon Jong Yang, Bok Yang Pyun, Ji Won Kwon, Myung Hyun Sohn, Kyung Won Kim, Kyu Earn Kim, Jinho Yu, Soo Jong Hong, Jung Hyun Kwon, Sung Won Kim, Tae Won Song, Woo Kyung Kim, Hyung Young Kim, You Hoon Jeon, Yong Ju Lee, Hae Ran Lee, Hye Young Kim, Youngmin Ahn, Hye Yung Yum, Dong In Suh, Hyun Hee Kim, Jin Tack Kim, Jeong Hee Kim, Yong Mean Park, Sooyoung Lee
PURPOSE: Although anaphylaxis is recognized as an important, life-threatening condition, data are limited regarding its triggers in different age groups. We aimed to identify anaphylaxis triggers by age in Korean children. METHODS: We performed a retrospective review of medical records for children diagnosed with anaphylaxis between 2009 and 2013 in 23 secondary or tertiary hospitals in South Korea. RESULTS: A total of 991 cases (mean age=5...
November 2016: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27372141/volatile-organic-compound-emissions-from-straw-amended-agricultural-soils-and-their-relations-to-bacterial-communities-a-laboratory-study
#3
Juan Zhao, Zhe Wang, Ting Wu, Xinming Wang, Wanhong Dai, Yujie Zhang, Ran Wang, Yonggan Zhang, Chengfei Shi
A laboratory study was conducted to investigate volatile organic compound (VOC) emissions from agricultural soil amended with wheat straw and their associations with bacterial communities for a period of 66days under non-flooded and flooded conditions. The results indicated that ethene, propene, ethanol, i-propanol, 2-butanol, acetaldehyde, acetone, 2-butanone, 2-pentanone and acetophenone were the 10 most abundant VOCs, making up over 90% of the total VOCs released under the two water conditions. The mean emission of total VOCs from the amended soils under the non-flooded condition (5924ng C/(kg·hr)) was significantly higher than that under the flooded condition (2211ng C/(kg·hr))...
July 2016: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/27183793/-the-inheritance-of-endosperm-storage-proteins-by-the-line-of-the-saratovskaya-29-cultivar-of-common-wheat-from-its-parental-forms
#4
L V Obukhova, V K Shumny
We ran a comparative analysis of storage proteins (gliadins, high- (HMW) and low-molecular-weight (LMW) glutenins, puroindolines, and exogenous α-amylase pest inhibitors) in the Saratovskaya 29 cultivar line from the collection of a genetic engineering laboratory, its parental forms (Albidum 24 and Lyutescens 55/11), and distant ancestors (Poltavka, Selivanovskiy Rusak, Sarroza, and tetraploid Beloturka). It was confirmed that the allelic states of storage proteins in the Gli-1, Gli-2 and Glu-1 loci originate from ancestral forms from the collection of the Vavilov Institute of Plant Industry...
January 2016: Genetika
https://www.readbyqxmd.com/read/26836300/molecular-and-cytogenetic-characterization-of-a-powdery-mildew-resistant-wheat-aegilops-mutica-partial-amphiploid-and-addition-line
#5
Cheng Liu, Guang-Rong Li, Wen-Ping Gong, Gen-Ying Li, Ran Han, Hao-Sheng Li, Jian-Min Song, Ai-Feng Liu, Xin-You Cao, Xiu-Sheng Chu, Zu-Jun Yang, Cheng-Yan Huang, Zhen-Dong Zhao, Jian-Jun Liu
Aegilops mutica Boiss., a diploid species (2n = 2x = 14, TT), has been rarely studied before. In this research, a hexaploid wheat (cv. Chinese Spring)-Ae. mutica partial amphiploid and a wheat-Ae. mutica addition line were characterized by chromosome karyotyping, FISH using oligonucleotides Oligo-pTa535-1, Oligo-pSc119.2-1, and (GAA)8 as probes, and EST-based molecular markers. The results showed that the partial amphiploid strain consisted of 20 pairs of wheat chromosomes and 7 pairs of Ae. mutica chromosomes, with both wheat 7B chromosomes missing...
2015: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/26657387/heavy-metal-contents-distribution-and-prediction-in-a-regional-soil-wheat-system
#6
Jing Ran, Dejian Wang, Can Wang, Gang Zhang, Hailin Zhang
The entry of heavy metals into the food chain is of concern for potential health risks. To investigate the spatial relationships of heavy metals in a regional soil-wheat system, 99 pairs of surface soil (0-15 cm) and wheat grain samples were collected from Changshu, China, a typical county in the Yangtze Delta region. Both soil and wheat grain samples were analyzed for total Cd, Cu, Ni, Pb, and Zn. DTPA-extractable metals and major physico-chemical properties were also determined for soil samples. Moderate accumulation of heavy metals was found in soils and wheat grains, especially Cd...
February 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/26329893/enhancing-the-thermostability-of-feruloyl-esterase-estf27-by-directed-evolution-and-the-underlying-structural-basis
#7
Li-chuang Cao, Ran Chen, Wei Xie, Yu-huan Liu
To improve the thermostability of EstF27, two rounds of random mutagenesis were performed. A thermostable mutant, M6, with six amino acid substitutions was obtained. The half-life of M6 at 55 °C is 1680 h, while that of EstF27 is 0.5 h. The Kcat/Km value of M6 is 1.9-fold higher than that of EstF27. The concentrations of ferulic acid released from destarched wheat bran by EstF27 and M6 at their respective optimal temperatures were 223.2 ± 6.8 and 464.8 ± 11.9 μM, respectively. To further understand the structural basis of the enhanced thermostability, the crystal structure of M6 is determined at 2...
September 23, 2015: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/26295045/a-comparison-of-food-supply-from-1984-to-2009-and-degree-of-dietary-westernization-in-taiwan-with-asian-countries-and-world-continents
#8
COMPARATIVE STUDY
Cheau-Jane Peng, Cheng-Yao Lin, How-Ran Guo
OBJECTIVE: To compare quality, quantity, and trends of food supply from 1984 to 2009 and degree of food westernization in Taiwan with Asian countries and world continents by using food balance data. METHODS: We compiled data from food balance sheets of Taiwan and Food and Agriculture Organization, including five continents and three most populated countries each in Eastern, Southern, and Southeastern Asia over the period 1984-2009. Quantity of food supply per capita was referenced to Taiwan food guides...
2015: BioMed Research International
https://www.readbyqxmd.com/read/25854602/biochemical-characterization-of-caffeoyl-coenzyme-a-3-o-methyltransferase-from-wheat
#9
Qing-Hu Ma, Hao-Ran Luo
TaCCoAOMT1 is located in wheat chromosome 7A and highly expressed in stem and root. It is important for lignin biosynthesis, and associated with stem maturity but not lodging resistance. Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are one important class of enzymes to carry out the transfer of the methyl group from S-adenosylmethionine to the hydroxyl group, and play important roles in lignin and flavonoids biosynthesis. In the present study, sequences for CCoAOMT from the wheat genome were analyzed...
July 2015: Planta
https://www.readbyqxmd.com/read/25766807/blasting-extrusion-processing-the-increase-of-soluble-dietary-fiber-content-and-extraction-of-soluble-fiber-polysaccharides-from-wheat-bran
#10
Xiaoguang Yan, Ran Ye, Ye Chen
In this study, soluble dietary fiber (SDF) content of wheat bran was significantly increased from 9.82 ± 0.16 (w/w, %) to 16.72 ± 0.28 (w/w, %) by a novel blasting extrusion processing with enhanced water retention capacity and the swelling capacity. In addition, a water-soluble polysaccharide (WBP) was isolated and extracted from extruded SDF. WBP was successfully purified from SDF by column chromatography systems with the average molecular weight (Mw) of 4.7 × 10(4)Da, containing arabinose, xylose, glucose, and galactose...
August 1, 2015: Food Chemistry
https://www.readbyqxmd.com/read/25158525/-soil-respiration-and-carbon-balance-in-wheat-field-under-conservation-tillage
#11
Sai Zhang, Long-Chang Wang, Zhao-Cun Huang, Hui-Juan Jia, Chun-Yan Ran
In order to study the characteristics of carbon sources and sinks in the winter wheat farmland ecosystem in southwest hilly region of China, the LI6400-09 respiratory chamber was adopted in the experiment conducted in the experimental field in Southwest University in Chongqing. The soil respiration and plant growth dynamics were analyzed during the growth period of wheat in the triple intercropping system of wheat-maize-soybean. Four treatments including T (traditional tillage), R (ridge tillage), TS (traditional tillage + straw mulching), and RS (ridge tillage + straw mulching) were designed...
June 2014: Huan Jing Ke Xue= Huanjing Kexue
https://www.readbyqxmd.com/read/25083910/elp3-is-involved-in-sexual-and-asexual-development-virulence-and-the-oxidative-stress-response-in-fusarium-graminearum
#12
Yoonji Lee, Kyunghun Min, Hokyoung Son, Ae Ran Park, Jin-Cheol Kim, Gyung Ja Choi, Yin-Won Lee
Fusarium graminearum is an important fungal plant pathogen that causes serious losses in cereal crop yields and mycotoxicoses in humans and livestock. In this study, we characterized an insertion mutant, Z39R9282, with pleiotropic defects in sexual development and virulence. We determined that the insertion occurred in a gene encoding an ortholog of yeast elongator complex protein 3 (ELP3). Deletion of elp3 led to significant defects in sexual and asexual development in F. graminearum. In the elp3 deletion mutant, the number of perithecia formed was reduced and maturation of perithecia was delayed...
December 2014: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/24734873/identification-and-characterization-of-micrornas-in-the-flag-leaf-and-developing-seed-of-wheat-triticum-aestivum-l
#13
Ran Han, Chao Jian, Jinyang Lv, Yan Yan, Qing Chi, Zhanjie Li, Qian Wang, Jin Zhang, Xiangli Liu, Huixian Zhao
BACKGROUND: MicroRNAs (miRNAs) regulate various biological processes in plants. Considerable data are available on miRNAs involved in the development of rice, maize and barley. In contrast, little is known about miRNAs and their functions in the development of wheat. In this study, five small RNA (sRNA) libraries from wheat seedlings, flag leaves, and developing seeds were developed and sequenced to identify miRNAs and understand their functions in wheat development. RESULTS: Twenty-four known miRNAs belonging to 15 miRNA families were identified from 18 MIRNA loci in wheat in the present study, including 15 miRNAs (9 MIRNA loci) first identified in wheat, 13 miRNA families (16 MIRNA loci) being highly conserved and 2 (2 MIRNA loci) moderately conserved...
2014: BMC Genomics
https://www.readbyqxmd.com/read/24697072/-effects-of-different-organic-manure-sources-and-their-combinations-with-chemical-fertilization-on-soil-nematode-community-structure-in-a-paddy-field-of-east-china
#14
Ting Liu, Cheng-Long Ye, Xiao-Yun Chen, Wei Ran, Qi-Rong Shen, Feng Hu, Hui-Xin Li
A comparative study was conducted to investigate the effects of different fertilization modes on the soil nematode community structure in a paddy field with paddy rice and wheat rotation in Jintan County (31 degrees 39'41.8" N, 119 degrees 28'23.5" E) of Jiangsu Province, East China. Six treatments were installed, i. e., no fertilization (CK), 100% chemical NPK fertilization (F), pig manure compost plus 50% chemical fertilization (PF), straw returning plus 100% chemical fertilization (SF), pig manure compost and straw returning plus 50% chemical fertilization (PSF), and application of commercial pig manure-inorganic complex fertilizer (PMF)...
December 2013: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/24562866/preparation-of-dufulin-imprinted-polymer-on-surface-of-silica-gel-and-its-application-as-solid-phase-extraction-sorbent
#15
Shan Shan Miao, Hua Zi Wang, Yi Chen Lu, Hao Ran Geng, Hong Yang
A new molecularly imprinted polymer (MIP) based on silica-gel surface was developed using Dufulin (Duf) as a template, methacrylic acid (MAA) as a functional monomer, ethyleneglycol dimethacrylate (EGDMA) as a crosslinker, and azodiisobutyronitrile (AIBN) as an initiator. The synthetic samples were characterized by the techniques of Fourier transmission infrared spectrometry (FT-IR) and scanning electron microscope (SEM). Batch experiments were performed to evaluate adsorption isotherms, adsorption kinetics and selective recognition of the MIP...
April 2014: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/24503781/identification-and-characterization-of-high-molecular-weight-glutenin-subunits-from-agropyron-intermedium
#16
Shuanghe Cao, Zhixin Li, Caiyan Gong, Hong Xu, Ran Yang, Shanting Hao, Xianping Wang, Daowen Wang, Xiangqi Zhang
High-molecular-weight glutenin subunit (HMW-GS) is a primary determinant of processing quality of wheat. Considerable progress has been made in understanding the structure, function and genetic regulation of HMW-GS in wheat and some of its related species, but less is known about their orthologs in Agropyron intermedium, a useful related species for wheat improvement. Here seven HMW-GSs in Ag. intermedium were identified using SDS-PAGE and Western blotting experiments. Subsequently, the seven genes (Glu-1Aix1 ∼ 4 and Glu-1Aiy1 ∼ 3) encoding the seven HMW-GSs were isolated using PCR technique with degenerate primers, and confirmed by bacterial expression and Western blotting...
2014: PloS One
https://www.readbyqxmd.com/read/24465530/responses-of-bacterial-communities-in-arable-soils-in-a-rice-wheat-cropping-system-to-different-fertilizer-regimes-and-sampling-times
#17
Jun Zhao, Tian Ni, Yong Li, Wu Xiong, Wei Ran, Biao Shen, Qirong Shen, Ruifu Zhang
Soil physicochemical properties, soil microbial biomass and bacterial community structures in a rice-wheat cropping system subjected to different fertilizer regimes were investigated in two seasons (June and October). All fertilizer regimes increased the soil microbial biomass carbon and nitrogen. Both fertilizer regime and time had a significant effect on soil physicochemical properties and bacterial community structure. The combined application of inorganic fertilizer and manure organic-inorganic fertilizer significantly enhanced the bacterial diversity in both seasons...
2014: PloS One
https://www.readbyqxmd.com/read/24369651/-extraction-and-analysis-of-solar-induced-chlorophyll-fluorescence-of-wheat-with-ground-based-hyperspectral-imaging-system
#18
Ran Wang, Zhi-gang Liu, Hai-kuan Feng, Pei-qi Yang, Qing-shan Wang, Zhuo-ya Ni
Dataset simulated with FluorMOD and images of wheat in heading stage taken by a ground-based hyperspectral imaging system with 3.3 nm spectral resolution and 0. 71-0. 74 nm spectral sampling interval were used test the feasibility and accuracy of three FLD methods (named FLD, 3FLD and iFLD). The results show that when spectral resolution is 3.3 nm, solar-induced chlorophyll fluorescence could be extracted effectively in O2-A band (around 760 nm) instead of O2-B band (around 687 nm). As to the extraction results of data with noises, both FLD and 3FLD are stabler than iFLD method...
September 2013: Guang Pu Xue Yu Guang Pu Fen Xi, Guang Pu
https://www.readbyqxmd.com/read/24191569/-effects-of-long-term-manure-and-crop-residues-incorporation-on-yield-and-phosphorus-saturation-in-a-paddy-soil
#19
Xiao Yan, De-Jian Wang, Gang Zhang, Jing Ran, Ji-Cheng Zheng
An 8-year field experiment was conducted in the Taihu Lake region of eastern China to investigate the effects of incorporation of straw and manure on the yield and phosphorus (P) accumulation in the paddy soil, and to evaluate the potential risk of P loss from soil to environment. The experiment had four fertilization treatments, i. e., chemical fertilizers alone (NPK), chemical fertilizers plus rice/wheat straw (NPK + S), chemical fertilizers plus 7.5 t x (hm2 x a)(-1) wet pig manure (NPK + M7.5), and chemical fertilizers plus 15...
August 2013: Huan Jing Ke Xue= Huanjing Kexue
https://www.readbyqxmd.com/read/24151183/development-of-a-molecularly-imprinted-polymer-for-prometryne-clean-up-in-the-environment
#20
Li Juan Guo, Jin Rong Qu, Shan Shan Miao, Hao Ran Geng, Hong Yang
Molecularly imprinted polymers (MIPs) are prepared on the surface of modified silica gel using prometryne as a template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as a crosslinker, and 2,2-azobisisobutyronitrile as an initiator. The structure of the MIPs was characterized using SEM and FTIR spectroscopy. The selectivity of the MIPs for the template molecule prometryne was proven by adsorption experiments. Highly selective SPE cartridges of MIP particles were developed and an optimized prometryne procedure was developed for the enrichment and clean-up of prometryne residues in water, soil, and wheat samples...
December 2013: Journal of Separation Science
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