Andrea Pinkeova, Adela Tomikova, Aniko Bertokova, Eva Fabinyova, Radka Bartova, Eduard Jane, Stefania Hroncekova, Karl-Dietrich Sievert, Roman Sokol, Michal Jirasko, Radek Kucera, Iris E Eder, Wolfgang Horninger, Helmut Klocker, Petra Ďubjaková, Juraj Fillo, Tomas Bertok, Jan Tkac
The glycoprofiling of two proteins, the free form of the prostate-specific antigen (fPSA) and zinc-α-2-glycoprotein (ZA2G), was assessed to determine their suitability as prostate cancer (PCa) biomarkers. The glycoprofiling of proteins was performed by analysing changes in the glycan composition on fPSA and ZA2G using lectins (proteins that recognise glycans, i.e. complex carbohydrates). The specific glycoprofiling of the proteins was performed using magnetic beads (MBs) modified with horseradish peroxidase (HRP) and antibodies that selectively enriched fPSA or ZA2G from human serum samples...
2024: PloS One