Nan Cao, Ruiyan Guo, Ping Song, Shaopeng Wang, Gang Liu, Jiye Shi, Lihua Wang, Min Li, Xiaolei Zuo, Xiurong Yang, Chunhai Fan, Mingqiang Li, Yueyue Zhang
Multienzyme assemblies mediated by multivalent interaction play a crucial role in cellular processes. However, the three-dimensional (3D) programming of an enzyme complex with defined enzyme activity in vitro remains unexplored, primarily owing to limitations in precisely controlling the spatial topological configuration. Herein, we introduce a nanoscale 3D enzyme assembly using a tetrahedral DNA framework (TDF), enabling the replication of spatial topological configuration and maintenance of an identical edge-to-edge distance akin to natural enzymes...
April 2, 2024: Nano Letters