keyword
MENU ▼
Read by QxMD icon Read
search

horseradish

keyword
https://www.readbyqxmd.com/read/28439996/energy-dispersive-spectroscopy-scanning-transmission-electron-microscope-observations-of-free-radical-production-in-human-polymorphonuclear-leukocytes-phagocytosing-non-opsonized-tannerella-forsythia
#1
Keiichi Moriguchi, Yoshiaki Hasegawa, Naoya Higuchi, Yukitaka Murakami, Fuminobu Yoshimura, Kazuhiko Nakata, Masaki Honda
We investigated the association between human polymorphonuclear leukocytes (PMNs) and non-opsonized Tannerella forsythia ATCC 43037 displaying a serum-resistant surface layer (S-layer). When PMNs were mixed with T. forsythia in suspension, the cells phagocytosed T. forsythia cells. Nitro blue tetrazolium (NBT) reduction, indicative of O2- production, was observed by light microscopy; cerium (Ce) perhydroxide deposition, indicative of H2 O2 production, was observed by electron microscopy. We examined the relationship between high-molecular-weight proteins of the S-layer and Ce reaction (for T...
April 24, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28437639/a-novel-biosensor-based-on-boronic-acid-functionalized-metal-organic-frameworks-for-the-determination-of-hydrogen-peroxide-released-from-living-cells
#2
Hongxia Dai, Wenjuan Lü, Xianwei Zuo, Qian Zhu, Congjie Pan, Xiaoying Niu, Juanjuan Liu, HongLi Chen, Xingguo Chen
In this work, we report a durable and sensitive H2O2 biosensor based on boronic acid functionalized metal-organic frameworks (denoted as MIL-100(Cr)-B) as an efficient immobilization matrix of horseradish peroxidase (HRP). MIL-100(Cr)-B features a hierarchical porous structure, extremely high surface area, and sufficient recognition sites, which can significantly increase HRP loading and prevent them from leakage and deactivation. The H2O2 biosensor can be easily achieved without any complex processing. Meanwhile, the immobilized HRP exhibited enhanced stability and remarkable catalytic activity towards H2O2 reduction...
April 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28434989/colorimetric-detection-of-microrna-based-hybridization-chain-reaction-for-signal-amplification-and-enzyme-for-visualization
#3
Na Ying, Taifan Sun, Zhibao Chen, Guangping Song, Bingyao Qi, Shengjun Bu, Xiuwei Sun, Jiayu Wan, Zehong Li
MicroRNAs (miRNAs) have key roles in gene expression and can be employed as biomarkers for early diagnosis of various diseases, especially cancers. Detection of miRNAs remains challenging and often requires detection platforms. Here, a horseradish peroxidase (HRP)-assisted hybridization chain reaction (HCR) for colorimetric detection of miR-155 was described. In the presence of target miRNA, the capture probe immobilized on the microplate sandwiched the target miR-155 with the 3' end of the reporter probe. Another exposed part of the RP at the 5'end triggered HCR producing double-stranded DNA polymers with multiple fluorescein isothiocyanates (FITC) for signal amplification...
April 20, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28432454/cobalt-tetradehydrocorrins-coordinated-by-imidazolate-like-histidine-in-the-heme-pocket-of-horseradish-peroxidase
#4
Koji Oohora, Ning Tang, Yoshitsugu Morita, Takashi Hayashi
Horseradish peroxidase was reconstituted with cobalt tetradehydrocorrin, rHRP(Co(TDHC)), as a structural analog of cobalamin coordinated with an imidazolate-like His residue, which is generally seen in native enzymes. In contrast to the previously reported cobalt tetradehydrocorrin-reconstituted myoglobin, rMb(Co(TDHC)), the HRP matrix was expected to provide strong axial ligation by His170 which has imidazolate character. rHRP(Co(II)(TDHC)) was characterized by EPR and its reaction with reductants indicates a negative shift of its redox potential compared to rMb(Co(TDHC))...
April 21, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28429896/production-of-a-conjugate-between-the-rk346-antigen-from-leishmania-infantum-and-the-horseradish-peroxidase-c-for-the-detection-of-rk346-antibodies
#5
Juan Rengifo-González, Yollyseth Medina-Mora, Sasha Silva-Barrios, María Elizabeth Márquez-Contreras, María Tibisay Ruiz, Ana J Cáceres, Juan Luis Concepción, Wilfredo Quiñones
It was designed and characterized a reporter system to be captured by an- tibodies bound to ELISA plates. The system was designed with the rK346 from Leishmania infantum, a highly antigenic and specific protein. The rK346 was coupled to the horseradish peroxidase C (HRPc) from Armoracia rusticana using glutaraldehyde or sulfo-SMCC. Gluta- raldehyde conjugation was performed in two steps. Separation of conjugates was carried out using a Sepharose S-200 in size exclusion chromatography (SEC); fractions were analyzed via HRPc activity and through ELISA plates sensitized with polyclonal anti-rK346 IgG puri- fied from rabbit serum...
June 2016: Investigación Clínica
https://www.readbyqxmd.com/read/28422254/a-dual-enzyme-inorganic-hybrid-nanoflower-incorporated-microfluidic-paper-based-analytic-device-%C3%AE-pad-biosensor-for-sensitive-visualized-detection-of-glucose
#6
Xueli Zhu, Jin Huang, Jinwen Liu, Hang Zhang, Jianhui Jiang, Ruqin Yu
A novel microfluidic paper-based analytic device (μPAD) biosensor is developed for sensitive and visualized detection of glucose. This biosensor is easily fabricated using the wax printing technique, with a hybrid nanocomplex composed of dual enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP) and Cu3(PO4)2 inorganic nanocrystals incorporated in the detection zones. The hybrid nanocomplex is found to exhibit a flower-like structure, which allows co-immobilization of these two enzymes in a biocompatible environment...
April 19, 2017: Nanoscale
https://www.readbyqxmd.com/read/28415842/spectrophotometric-quantification-of-peroxidase-with-p-phenylene-diamine-for-analyzing-peroxidase-encapsulating-lipid-vesicles
#7
Ya Zhang, Yannick R F Schmid, Sandra Luginbühl, Qiang Wang, Petra S Dittrich, Peter Walde
A spectrophotometric assay for the determination of horseradish peroxidase (HRP) in aqueous solution with p-phenylenediamine (PPD, benzene-1,4-diamine) as electron donor substrate and hydrogen peroxide (H2O2) as oxidant was developed. The oxidation of PPD by HRP/H2O2 leads to the formation of Bandrowski's base ((3E,6E)-3,6-bis[(4-aminophenyl)imino]cyclohexa-1,4-diene-1,4-diamine), which can be quantified by following the increase in absorbance at 500 nm. The assay was applied for monitoring the activity of HRP inside ≈180 nm-sized lipid vesicles (liposomes), prepared from POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and purified by size exclusion chromatography...
April 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28412630/phenol-remediation-by-peroxidase-from-an-invasive-mesquite-turning-an-environmental-wound-into-wisdom
#8
Savita Singh, Ruchi Mishra, Radhey Shyam Sharma, Vandana Mishra
The present study examines mesquite (Prosopis juliflora), an invasive species, to yield peroxidase that may reduce hazards of phenolics to living organisms. As low as 0.3U of low-purity mesquite peroxidase (MPx) efficiently remove phenol and chlorophenols (90-92%) compared with Horseradish peroxidase (HRP) (40-60%). MPx shows a very high removal efficiency (40-50%) at a wide range of pH (2-9) and temperature (20-80°C), as opposed to HRP (15-20%). At a high-level of the substrate (2.4mM) and without the addition of PEG, MPx maintains a significant phenolic removal (60-≥92%) and residual activity (∼25%)...
April 4, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28411649/nanoarmoring-of-enzymes-by-interlocking-in-cellulose-fibers-with-poly-acrylic-acid
#9
Caterina M Riccardi, Rajeswari M Kasi, Challa V Kumar
A simple method for interlocking glucose oxidase (GOx) and horseradish peroxidase (HRP) in cellulose fibers using poly(acrylic acid) (PAA) as an armor around the enzyme, without any need for activation of the cellulose support, is reported here. The resulting enzyme paper is an inexpensive, stable, simple, wearable, and washable biosensor. PAA functions as a multifunctional tether to interlock the enzyme molecules around the paper fibers so that the enzymes are protected against thermal/chemical denaturation and not released from the paper when washed with a detergent...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28411637/armored-enzyme-nanohybrids-and-their-catalytic-function-under-challenging-conditions
#10
Omkar V Zore, Rajeswari M Kasi, Challa V Kumar
Synthesis and characterization of highly stable and functional bienzyme-polymer triads assembled on layered graphene oxide (GO) are described here. Glucose oxidase (GOx) and horseradish peroxidase (HRP) were used as model enzymes and polyacrylic acid (PAA) as model polymer to armor the enzymes. PAA-armored GOx and HRP covalent conjugates were further protected from denaturation by adsorption onto GO nanosheets. Structure and morphology of this enzyme-polymer-nanosheet hybrid biocatalyst (GOx-HRP-PAA/GO) were confirmed by agarose gel electrophoresis, zeta potential, circular dichroism, and transmission electron microscopy...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28411223/modified-bacteriophage-s16-long-tail-fiber-proteins-for-rapid-and-specific-immobilization-and-detection-of-salmonella-cells
#11
Jenna M Denyes, Matthew Dunne, Stanislava Steiner, Maximilian Mittelviefhaus, Agnes Weiss, Herbert Schmidt, Jochen Klumpp, Martin J Loessner
Bacteriophage-based assays and biosensors rival traditional antibody-based immunoassays for detection of low-level Salmonella contaminations. In this study, we harnessed the binding specificity of the long tail fiber (LTF) from bacteriophage S16 as an affinity molecule for immobilization, enrichment, and detection of Salmonella We demonstrate that paramagnetic beads (MBs) coated with recombinant gp37-gp38 LTF complexes (LTF-MBs) are highly effective tools for rapid affinity magnetic separation and enrichment of Salmonella Within 45 minutes, the LTF-MBs consistently captured over 95% of Salmonella Typhimurium cells from suspensions containing 10-10(5) CFU...
April 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28410480/chemiluminescence-noncompetitive-immunoassay-based-on-microchip-electrophoresis-for-the-determination-of-%C3%AE-subunit-of-human-chorionic-gonadotropin
#12
Shuting Li, Tingzheng Yang, Jingjin Zhao, Yong Huang, Shulin Zhao
In this work, a microchip-electrophoresis chemiluminescence (MCE-CL)-based immunoassay method was established for the determination of β-subunit of human chorionic gonadotropin (β-HCG). This approach uses MCE-CL assay as a platform. First, the β-HCG antigen (Ag) binds to excess horseradish peroxidase (HRP)-labeled anti-β-HCG antibodies (Ab*) to form an immune complex (Ag-Ab(*)). Subsequently, the obtained Ag-Ab(*) complex and unreacted Ab(*) were separated by MCE, and detected by CL. The CL intensity (peak high) of Ag-Ab(*) was used to estimate β-HCG concentration...
March 29, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28399040/a-new-enzyme-linked-immunosorbent-assay-elisa-for-a-total-anti-t-lymphocyte-globulin-determination-development-analytical-validation-and-clinical-application
#13
M Montagna, G La Nasa, M E Bernardo, E Piras, M A Avanzini, M Regazzi, F Locatelli
BACKGROUND: Anti-T lymphocyte globulin (ATLG) modulates the alloreactivity of T lymphocytes, reducing the risk of immunological post-transplant complications, in particular rejection and graft-versus-host disease (GvHD), after allogeneic hematopoietic stem cell transplantation (HSCT).We developed and validated a new enzyme-linked immunosorbent assay (ELISA) method to measure serum levels of total ATLG and evaluate the pharmacokinetics (PK) of the drug in children with β-Thalassemia, receiving allogeneic HSCT...
April 7, 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28393943/enhancing-coupled-enzymatic-activity-by-conjugating-one-enzyme-to-a-nanoparticle
#14
James N Vranish, Mario G Ancona, Eunkeu Oh, Kimihiro Susumu, Igor L Medintz
Enzymes have long been a prime research target for the commercial production of commodity and specialty chemicals, design of sensing devices, and the development of therapeutics and new chemical processes. Industrial applications for enzymes can potentially be enhanced by enzyme immobilization which often allows for increased enzyme stability, facile product purification, and minimized substrate diffusion times in multienzymatic cascades, but this is usually at the cost of a significant decrease in catalytic rates...
April 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28393929/an-elegant-analysis-of-white-spot-syndrome-virus-using-a-graphene-oxide-methylene-blue-based-electrochemical-immunosensor-platform
#15
Anusha Natarajan, K S Shalini Devi, Sudhakaran Raja, Annamalai Senthil Kumar
White spot syndrome virus (WSSV) is a major devastating virus in aquaculture industry. A sensitive and selective diagnostic method for WSSV is a pressing need for the early detection and protection of the aquaculture farms. Herein, we first report, a simple electrochemical immunosensor based on methylene blue dye (MB) immobilized graphene oxide modified glassy carbon electrode (GCE/GO@MB) for selective, quick (35 ± 5 mins) and raw sample analysis of WSSV. The immunosensor was prepared by sequential modification of primary antibody, blocking agent (bovine serum album), antigen (as vp28 protein), secondary antibody coupled with horseradish peroxidase (Ab2-HRP) on the GCE/GO@MB...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28393516/ultrasensitive-electrochemical-detection-of-glycoprotein-based-on-boronate-affinity-sandwich-assay-and-signal-amplification-with-functionalized-sio2-au-nanocomposites
#16
Min You, Shuai Yang, Wanxin Tang, Fan Zhang, Pin-Gang He
Herein we propose a multiple signal amplification strategy designed for ultrasensitive electrochemical detection of glycoproteins. This approach introduces a new type of boronate-affinity sandwich assay (BASA), which was fabricated by using gold nanoparticles combined with reduced graphene oxide (AuNPs-GO) to modify sensing surface for accelerating electron transfer, the composite of molecularly imprinted polymer (MIP) including 4-vinylphenylboronic acid (VPBA) for specific capturing glycoproteins, and SiO2 nanoparticles carried gold nanoparticles (SiO2@Au) labeled with 6-ferrocenylhexanethiol (FcHT) and 4-mercaptophenylboronic acid (MPBA) (SiO2@Au/FcHT/MPBA) as tracing tag for binding glycoprotein and generating electrochemical signal...
April 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28390975/natural-isothiocyanates-express-antimicrobial-activity-against-developing-and-mature-biofilms-of-pseudomonas-aeruginosa
#17
Stefan J Kaiser, Nico T Mutters, Brigitte Blessing, Frank Günther
BACKGROUND: The antimicrobial properties of natural isothiocyanates (ITCs) found in plants such as nasturtium (Tropaeolum majus) and horseradish (Armoracia rusticana), and the need of new chemotherapeutic options for treatment of infections caused by multidrug-resistant and biofilm-forming Gram-negative bacteria such as Pseudomonas aeruginosa (Pa), led us to evaluate the effects of three major ITCs, allylisothiocyanate (AITC), benzylisothiocyanate (BITC), and phenylethyl-isothiocyanate (PEITC), and a mixture (ITCM) adapted to the ITC composition after release of active components out of natural sources...
April 5, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28390486/colorimetric-biosensor-for-the-assay-of-paraoxon-in-environmental-water-samples-based-on-the-iodine-starch-color-reaction
#18
Liangqia Guo, Zhihong Li, Huan Chen, Yarong Wu, Ling Chen, Zhiping Song, Tianran Lin
In this work, a new colorimetric biosensor for the assay of paraoxon was developed via the conventional iodine-starch color reaction and multi-enzyme cascade catalytic reactions. In the presence of acetylcholine chloride, acetylcholinesterase (AChE) and choline oxidase (ChO) catalyzed the formation of H2O2, which then activated horseradish peroxidase (HRP) to catalyze the oxidation of KI to produce an iodine-starch color reaction. Upon exposure to paraoxon, the catalytic activity of AChE was inhibited and less H2O2 generated, resulting in a decrease in the production of I2 and a drop in the intensity of solution color...
May 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28390321/increased-electrocatalyzed-performance-through-high-content-potassium-doped-graphene-matrix-and-aptamer-tri-infinite-amplification-labels-strategy-highly-sensitive-for-matrix-metalloproteinases-2-detection
#19
Xiang Ren, Tong Zhang, Dan Wu, Tao Yan, Xuehui Pang, Bin Du, Wanruo Lou, Qin Wei
Herein, a super-labeled immunoassay was fabricated for matrix metalloproteinases-2 detection. A self-corrosion ITO micro circuit board was designed in this sensing platform to reduce the random error in the same testing condition, and the self-constructed sensing platform is portable with a cheap price. The K-modified graphene (K-GS) was utilized as the matrix material, which was synthesized well by phenylate and phenanthrene through the polar bond of nonpolar molecule phenylate and the π-π interaction for the first time...
April 2, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28390088/functional-dual-hydrophilic-dendrimer-modified-metal-organic-framework-for-the-selective-enrichment-of-n-glycopeptides
#20
Yanan Wang, Jiaxi Wang, Mingxia Gao, Xiangmin Zhang
Analysis of protein glycosylation remains a significant challenge due to the low abundance of glycoproteins or N-glycopeptides. Here we have synthesized an amino-functionalized metal-organic framework (MOF) MIL-101(Cr)-NH2 whose surface is grafted with a hydrophilic dendrimer poly(amidoamine) (PAMAM) for N-glycopeptide enrichment based on the hydrophilic interactions. The selected substrate MOF MIL-101(Cr) owns high surface area which provides nice support for peptide adsorption. In addition, the MOF displayed a good hydrophilic property after being modified with amino groups...
April 8, 2017: Proteomics
keyword
keyword
48415
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"