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https://www.readbyqxmd.com/read/28637348/hydrogen-peroxide-sensing-based-on-inner-surfaces-modification-of-solid-state-nanopore
#1
Libo Zhu, Dejian Gu, Quanjun Liu
There are many techniques for the detection of molecules. But detection of molecules through solid-state nanopore in a solution is one of the promising, high-throughput, and low-cost technology used these days. In the present investigation, a solid-state nanopore platform was fabricated for the detection of hydrogen peroxide (H2O2), which is not only a label free product but also a significant participant in the redox reaction. We have successfully fabricated silicon nitride (Si3N4) nanopores with diameters of ~50 nm by using a focused Ga ion beam, the inner surface of the nanopore has been modified with horseradish peroxidase (HRP) by employing carbodiimide coupling chemistry...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28636187/dual-labeled-chemiluminescence-enzyme-immunoassay-for-simultaneous-measurement-of-total-prostate-specific-antigen-tpsa-and-free-prostate-specific-antigen-fpsa
#2
Lixia Zhao, Dan Wang, Gen Shi, Ling Lin
The specificity for early diagnostic of prostate-specific antigen (PSA) is low because the current technology mostly allows the detection of only one biomarker at one time. In this work, a dual-labeled chemiluminescence enzyme immunoassay (CLEIA) for simultaneous measurement of total PSA (TPSA) and free PSA (FPSA) was proposed. Anti-PSA McAb (Mab1) was immobilized on a microplate as the solid phase, horseradish peroxidase (HRP)-labeled anti-TPSA monoclonal antibody (McAb2) and alkaline phosphatase (ALP)-labeled anti-FPSA McAb3 were used as detection antibodies...
June 21, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28628845/serum-antioxidant-capacity-and-oxidative-damage-in-clinical-and-subclinical-canine-ehrlichiosis
#3
Camila Peres Rubio, Zeki Yilmaz, Silvia Martínez-Subiela, Meric Kocaturk, Josefa Hernández-Ruiz, Ebru Yalcin, Asta Tvarijonaviciute, Damián Escribano, Jose Joaquín Ceron
The objective of this study was to evaluate and compare the antioxidant response and the products of oxidative damage analysed by various assays in clinical and subclinical canine monocytic ehrlichiosis (CME). For this purpose, four assays to measure the total serum antioxidant capacity (TAC), such as the cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), trolox equivalent antioxidant capacity (TEAC) using acidic medium (TEACA), and the TEAC using the horseradish peroxidase (TEACH) were used...
June 12, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28620918/development-of-a-h2-o2-sensitive-quantum-dots-based-fluorescent-sandwich-elisa-for-sensitive-detection-of-bovine-%C3%AE-lactoglobulin-by-monoclonal-antibody
#4
Shengfa He, Xin Li, Jinyan Gao, Ping Tong, Hongbing Chen
BACKGROUND: Bovine β-lactoglobulin (BLG) is the major allergen in cows' milk, and specific epitope play a key role in food allergy. Developing a method specifically bind to IgE epitope is necessary for testing BLG and its allergenic residues. RESULTS: The monoclonal antibody (1G9) specific to IgE linear epitope for BLG was identified as high affinity and specificity. Based on 1G9, a sensitive fluorescent sandwich enzyme-linked immunosorbent assay (sELISA) was successfully developed using catalase-mediated fluorescence quenching of thiolated CdTe quantum dots in the presence of hydrogen peroxide as fluorescent signal output...
June 16, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28612283/cell-laden-microgel-prepared-using-a-biocompatible-aqueous-two-phase-strategy
#5
Yang Liu, Natalia Oshima Nambu, Masahito Taya
Microfluidic methods are frequently used to produce cell-laden microgels for various biomedical purposes. Such microfluidic methods generally employ oil-water systems. The poor distribution of crosslinking reagents in the oil phase limits the available gelation strategies. Extracting the microgel from the oil-phase also reduces its production efficiency. In this study, an aqueous two-phase system (ATPS) involving dextran (DEX) and polyethylene glycol (PEG) was used to prepare cell-laden microgel. This avoided the problems associated with an oil phase...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28600990/dual-signal-amplification-strategy-of-au-nanopaticles-zno-nanorods-hybridized-reduced-graphene-nanosheet-and-multienzyme-functionalized-au-zno-composites-for-ultrasensitive-electrochemical-detection-of-tumor-biomarker
#6
Xian Fang, Junfeng Liu, Jing Wang, Hong Zhao, Hongxuan Ren, Zengxi Li
Herein, a dual signal amplification strategy was employed in fabricating ultrasensitive electrochemical immunosensor for alpha fetoprotein (AFP) detection, which was realized by utilizing of ZnO nanorods/Au nanopaticles hybridized reduced graphene nanosheet (Au/ZnO/RGO) and horseradish-peroxidase (HRP) bioconjugated detection antibody (Ab2) functionalized Au@ZnO (Ab2/HRP-Au@ZnO). During the fabrication of the immunosensor, a new kind of multiple-head surfactants CxN3 with different alkyl chain length played important roles such as acting as the surfactants of Au/ZnO/RGO and the reductant agents of Au@ZnO composite...
May 31, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28596935/green-synthesis-of-enzyme-metal-organic-framework-composites-with-high-stability-in-protein-denaturing-solvents
#7
Xiaoling Wu, Cheng Yang, Jun Ge
OBJECTIVES: Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study. RESULTS: Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28596006/fabrication-of-single-and-bundled-filament-like-tissues-using-biodegradable-hyaluronic-acid-based-hollow-hydrogel-fibers
#8
Mehdi Khanmohammadi, Shinji Sakai, Masahito Taya
Hydrogel fibers with biodegradable and biocompatible features are useful for the fabrication of filament-like tissues. We developed cell-laden hyaluronic acid (HA)-based hollow hydrogel fibers to create single and bundled filament-like tissues. The cell-laden fibers were fabricated by crosslinking phenolic-substituted hyaluronic acid (HA-Ph) in an aqueous solution containing cells through a horseradish peroxidase (HRP)-catalyzed reaction in the presence of catalase by extruding the solution in ambient flow of an aqueous solution containing H2O2...
June 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28588614/optimization-of-aflatoxin-b1-aptasensing
#9
Marzieh Jafari, Mohsen Rezaei, Heibatullah Kalantari, Maryam Tabarzad, Bahram Daraei
Combination of aptamers with DNAzymes attracted intense attention for development of DNA-based biosensors for detection of mycotoxins. In the present study a combination of aflatoxin B1 specific aptamer and HRP- (horseradish peroxidase-) mimicking DNAzyme was optimized for detecting aflatoxin B1. Detecting approach is based on the binding affinity of aflatoxin B1 to its specific aptamer and conversion of substrate to a detectable colorimetric signal by a linked DNAzyme. Compared to conventional methods for aflatoxin B1 detection, DNA-based assay has the advantages of low cost, long-term stability, and rapid, simple, and user-friendly steps...
2017: Journal of Toxicology
https://www.readbyqxmd.com/read/28587260/an-enzyme-induced-novel-biosensor-for-the-sensitive-electrochemical-determination-of-isoniazid
#10
Rajasekhar Chokkareddy, Natesh Kumar Bhajanthri, Gan G Redhi
In this present work, a glassy carbon electrode (GCE) was modified primarily with multiwalled carbon nanotubes (MWCNTs) and a composite of MWCNTs and titanium oxide nanoparticles (TiO₂NPs). The enzyme horseradish peroxidase (HRP) was immobilized to enhance the sensing ability of GCE. The proposed biosensor was used for the sensitive determination of isoniazid (INZ) in various pharmaceutical samples. The electrochemical behaviour of the developed MWCNT-TiO₂NPs-HRP-GCE biosensor was studied by using cyclic voltammetry (CV) and differential pulse voltammetric (DPV) techniques...
June 5, 2017: Biosensors
https://www.readbyqxmd.com/read/28582709/electrodepositable-alginate-membranes-for-enzymatic-sensors-an-amperometric-glucose-biosensor-for-whole-blood-analysis
#11
A Márquez, C Jiménez-Jorquera, C Domínguez, X Muñoz-Berbel
Simple and disposable point of care systems are usually the best solution for chronic patients to get a rapid diagnosis in home care context. However, their main drawback relies on the poor reliability derived from the low stability of the bio-recognition elements and low quality of the transducers. In the current work, we study the use of electrodeposited calcium alginate hydrogels as a biocompatible matrix in the development of enzymatic amperometric biosensors for whole blood analysis, to enhance the enzymes stability and to protect the transducer from biofouling...
May 30, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28573321/an-efficient-direct-competitive-nano-elisa-for-residual-bsa-determination-in-vaccines
#12
Qian-Long Wang, Jie Li, Xing-De Li, Wan-Jun Tao, Li-Sheng Ding, Pei Luo, Lin-Sen Qing
A simple, fast, and highly sensitive direct competitive enzyme-linked immunosorbent assay (ELISA) based on bovine serum albumin (BSA) antigen labeled amine-terminated silicon dioxide (SiO2-NH-BSA) nanoparticles was developed to determine residual BSA in vaccines. As nano-ELISA using nanomaterials with a very high surface-to-volume ratio has emerged as a promising strategy, SiO2-NH-BSA nanoparticles were prepared in this study by the coupling of BSA to SiO2 nanoparticles modified with amidogen, followed by the quantification of BSA via a direct competitive binding of BSA-antigen-labeled SiO2 nanoparticles to anti-BSA antibody conjugated with horseradish peroxidase...
June 2, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28551395/a-sandwich-elisa-for-porcine-alpha-1-acid-glycoprotein-pagp-orm-1-and-further-demonstration-of-its-use-to-evaluate-growth-potential-in-newborn-pigs
#13
T J Caperna, A E Shannon, M Stoll, S Kahl, L A Blomberg, J L Vallet, T G Ramsay
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen. The antiserum was affinity purified, and a portion of the purified antibody fraction was labeled with horseradish peroxidase. Porcine AGP protein was used as a standard, whereas commercially available buffers and reagents were utilized throughout the assay...
April 8, 2017: Domestic Animal Endocrinology
https://www.readbyqxmd.com/read/28551226/effect-of-pulsed-light-on-activity-and-structural-changes-of-horseradish-peroxidase
#14
Bei Wang, Yanyan Zhang, Chandrasekar Venkitasamy, Bengang Wu, Zhongli Pan, Haile Ma
The objective of this research was to investigate the effects of pulsed light on the activity and structure of horseradish peroxidase in buffer solution. Enzyme residual activities were measured. Surface topography, secondary, and tertiary structures of horseradish peroxidase were determined using atomic force microscopy (AFM), Raman spectroscopy, and fluorescence spectroscopy, respectively. Results showed that a complete inactivation of horseradish peroxidase was achieved by application of 10 pulses of pulsed light treatment at an intensity of 500J/pulse...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28545731/enzyme-linked-immunoassay-based-on-imprinted-microspheres-for-the-detection-of-sulfamethazine-residue
#15
Dapeng Peng, Zhaozhou Li, Yulian Wang, Zhenli Liu, Feng Sheng, Zonghui Yuan
This study attempts to develop an enzyme-linked immunoassay (ELISA) using a molecularly imprinted polymer (MIP) as an artificial recognition element. The MIP microspheres were prepared using precipitation polymerization with SM2 as the template molecule, methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross-linker. After the microspheres were coated in microtiter plate wells, the molecular imprinting ELISA (MI-ELISA) method was established based on the direct competition between free SM2 and horseradish peroxidase (HRP)-labelled SM2 in heterogeneous mode...
May 8, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28545531/selection-and-characterization-of-alanine-racemase-inhibitors-against-aeromonas-hydrophila
#16
Yaping Wang, Chao Yang, Wen Xue, Ting Zhang, Xipei Liu, Jiansong Ju, Baohua Zhao, Dong Liu
BACKGROUND: Combining experimental and computational screening methods has been of keen interest in drug discovery. In the present study, we developed an efficient screening method that has been used to screen 2100 small-molecule compounds for alanine racemase Alr-2 inhibitors. RESULTS: We identified ten novel non-substrate Alr-2 inhibitors, of which patulin, homogentisic acid, and hydroquinone were active against Aeromonas hydrophila. The compounds were found to be capable of inhibiting Alr-2 to different extents with 50% inhibitory concentrations (IC50) ranging from 6...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28537384/cysteine-functionalized-metal-organic-framework-facile-synthesis-and-high-efficient-enrichment-of-n-linked-glycopeptides-in-cell-lysate
#17
Wen Ma, Linnan Xu, Xianjiang Li, Sensen Shen, Mei Wu, Yu Bai, Huwei Liu
Cysteine-functionalized metal-organic framework (MOF) was synthesized via a common and facile two-step method of in situ loading of Au nanoparticles on amino-derived MOF followed by l-cysteine (Cys) immobilization. Owing to the large specific surface area and ultrahigh hydrophilicity of this nanocomposite, excellent performance was observed in the enrichment of N-linked glycopeptides in both model glycoprotein and HeLa cell lysate. By using this nanocomposite, 16 and 31 glycopeptides were efficiently extracted from digest of horseradish peroxidase (HRP) and human serum immunoglobulin G (IgG), respectively...
June 1, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28531992/boronate-functionalized-hydrogel-as-a-novel-biosensing-interface-for-the-glycated-hemoglobin-a1c-hba1c-based-on-the-competitive-binding-with-signaling-glycoprotein
#18
Yong Duk Han, Ka Ram Kim, Yoo Min Park, Seung Yeon Song, Yong Ju Yang, Kangsun Lee, Yunhee Ku, Hyun C Yoon
According to recent increases in public healthcare costs associated with diabetes mellitus, the development of new glycemic monitoring techniques based on the biosensing of glycated hemoglobin A1c (HbA1c), a promising long-term glycemic biomarker, has become a major challenge. In the development of HbA1c biosensors for point-of-care applications, the selection of an effective biorecognition layer that provides a high reaction yield and specificity toward HbA1c is regarded as the most significant issue. To address this, we developed a novel HbA1c biosensing interfacial material by the integration of boronate hydrogel with glass fiber membrane...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28525854/sensitive-sulfide-ion-detection-by-optofluidic-catalytic-laser-using-horseradish-peroxidase-hrp-enzyme
#19
Chaoyang Gong, Yuan Gong, Maung Kyaw Khaing Oo, Yu Wu, Yunjiang Rao, Xiaotian Tan, Xudong Fan
We report an optofluidic catalytic laser for sensitive sulfide ion detection. In the catalytic reaction, horseradish peroxidase (HRP) enzyme is used for catalyzing the non-fluorescent substrate, 10-Acetyl-3,7-dihydroxyphenox-azine (ADHP), to produce highly fluorescent resorufin, which was used as gain medium for lasing. Using sulfide ions as inhibitors, the catalytic reaction slows down, resulting in a delay in the lasing onset time, which is used as the sensing signal. The sensing mechanism of the catalytic laser is theoretically analyzed and the performance is experimentally characterized...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28523913/expression-and-characterization-of-windmill-palm-tree-trachycarpus-fortunei-peroxidase-by-pichia-pastoris
#20
Boting Wen, Margaret R Baker, Hongwei Zhao, Zongjun Cui, Qing X Li
Currently, commercial plant peroxidases are all native and are isolated from plants such as horseradish and soybean. No recombinant plant peroxidase products have been available on the commercial market. The gene encoding peroxidase was cloned from windmill palm tree leaves. The codon-optimized gene was transformed into Pichia pastoris for expression. The recombinant windmill palm tree peroxidase (rWPTP) expressed by P. pastoris showed high stability under pH 2-10 and temperatures up to 70 °C to many metallic salts and organic solvents...
May 26, 2017: Journal of Agricultural and Food Chemistry
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