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fumaric acid

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https://www.readbyqxmd.com/read/29143132/thermostable-and-highly-specific-l-aspartate-oxidase-from-thermococcus-litoralis-dsm-5473-cloning-overexpression-and-enzymological-properties
#1
Tsubasa Washio, Tadao Oikawa
We successfully expressed the L-aspartate oxidase homolog gene (accession no: OCC_06611) of Thermococcus litoralis DSM 5473 in the soluble fraction of Escherichia coli BL21 (DE3) using a pET21b vector with 6X His tag at its C-terminus. The gene product (Tl-LASPO) showed L-aspartate oxidase activity in the presence of FAD in vitro, and this report is the first that details an L-aspartate oxidase derived from a Thermococcus species. The homologs of Tl-LASPO existed mainly in archaea, especially in the genus of Thermococcus, Pyrococcus, Sulfolobus, and Halobacteria...
November 15, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29140245/bacterial-fumarase-and-l-malic-acid-are-evolutionary-ancient-components-of-the-dna-damage-response
#2
Esti Singer, Yardena Bh Silas, Sigal Ben-Yehuda, Ophry Pines
Fumarase is distributed between two compartments of the eukaryotic cell. The enzyme catalyses the reversible conversion of fumaric to L-malic acid in mitochondria as part of the tricarboxylic acid (TCA) cycle, and in the cytosol/nucleus as part of the DNA damage response (DDR). Here we show that fumarase of the model prokaryote Bacillus subtilis (Fum-bc) is induced upon DNA damage, co-localized with the bacterial DNA and is required for the DDR. Fum-bc can substitute for both eukaryotic functions in yeast. Furthermore, we found that the fumarase dependent intracellular signaling of the B...
November 15, 2017: ELife
https://www.readbyqxmd.com/read/29133864/effects-of-microgravity-on-osteoblast-mitochondria-a-proteomic-and-metabolomics-profile
#3
Anna Michaletti, Magda Gioia, Umberto Tarantino, Lello Zolla
The response of human primary osteoblasts exposed to simulated microgravity has been investigated and analysis of metabolomic and proteomic profiles demonstrated a prominent dysregulation of mitochondrion homeostasis. Gravitational unloading treatment induced a decrease in mitochondrial proteins, mainly affecting efficiency of the respiratory chain. Metabolomic analysis revealed that microgravity influenced several metabolic pathways; stimulating glycolysis and the pentose phosphate pathways, while the Krebs cycle was interrupted at succinate-fumarate transformation...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29130499/combined-metabolomic-and-correlation-networks-analyses-reveal-fumarase-insufficiency-altered-amino-acids-metabolism
#4
Entai Hou, Xian Li, Zerong Liu, Fuchang Zhang, Zhongmin Tian
Fumarase catalyzes the interconversion of fumarate and L-malate in the tricarboxylic acid cycle. Fumarase insufficiencies were associated with increased level of fumarate and decreased level of malate and exacerbated salt-induced hypertension. To gain insights into the metabolism profiles that induced by fumarase insufficiency and identify key regulatory metabolites, we applied a GC-MS based metabolomics platform coupled with a network approach to analyze fumarase insufficient HUVEC cells and negative controls...
November 11, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29119686/ketone-body-3-hydroxybutyrate-mimics-calorie-restriction-via-the-nrf2-activator-fumarate-in-the-retina
#5
Yusuke Izuta, Toshihiro Imada, Ryuji Hisamura, Erina Oonishi, Shigeru Nakamura, Emi Inagaki, Masataka Ito, Tomoyoshi Soga, Kazuo Tsubota
Calorie restriction (CR) being the most robust dietary intervention provides various health benefits. D-3-hydroxybutyrate (3HB), a major physiological ketone, has been proposed as an important endogenous molecule for CR. To investigate the role of 3HB in CR, we investigated potential shared mechanisms underlying increased retinal 3HB induced by CR and exogenously applied 3HB without CR to protect against ischemic retinal degeneration. The repeated elevation of retinal 3HB, with or without CR, suppressed retinal degeneration...
November 9, 2017: Aging Cell
https://www.readbyqxmd.com/read/29114862/fumaric-acid-esters-in-combination-with-a-6-week-course-of-narrow-band-uvb-provides-for-an-accelerated-response-as-compared-to-fumaric-acid-esters-monotherapy-in-patients-with-moderate-to-severe-plaque-psoriasis-a-randomized-prospective-clinical-study
#6
S Tzaneva, A Geroldinger, H Trattner, A Tanew
BACKGROUND: Fumaric acid esters are (FAE) safe and effective in patients with moderate to severe psoriasis but have a slow onset of action. A short-term combination with narrow-band UVB (NB UVB) may substantially accelerate the therapeutic response in the induction phase of treatment. OBJECTIVES: To assess the synergistic effect of a 6-week course of NB UVB phototherapy in addition to fumaric acid esters in adult patients with moderate to severe plaque psoriasis...
November 8, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/29112902/development-of-a-stability-indicating-hplc-method-for-simultaneous-determination-of-ten-related-substances-in-vonoprazan-fumarate-drug-substance
#7
Zhiqiang Luo, Aoxue Liu, Yang Liu, Guopeng Wang, Xinjing Chen, Hao Wang, Mengwei Li, Haili Zhang, Yanhua Qiu, Huaqiang Zhai
Vonoprazan fumarate is a novel potassium-competitive acid blocker for the treatment of acid-related diseases. In the present study, a simple, fast, and economic reversed-phase liquid chromatography (LC) method was developed for the analysis of ten related substances (raw materials, by-products and degradants) in vonoprazan fumarate. The optimized separation was performed on a Phenomenex Kinetex EVO C18 (250mm×4.6mm, 5.0μm) column. The mobile phase consisted of (A) 0.03M sodium phosphate buffer (pH adjusted to 6...
November 4, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29101585/comparative-transcriptomic-insights-into-the-mechanisms-of-electron-transfer-in-geobacter-co-cultures-with-activated-carbon-and-magnetite
#8
Shiling Zheng, Fanghua Liu, Meng Li, Leilei Xiao, Oumei Wang
Both activated carbon and magnetite have been reported to promote the syntrophic growth of Geobacter metallireducens and Geobacter sulfurreducens co-cultures, the first model to show direct interspecies electron transfer (DIET); however, differential transcriptomics of the promotion on co-cultures with these two conductive materials are unknown. Here, the comparative transcriptomic analysis of G. metallireducens and G. sulfurreducens co-cultures with granular activated carbon (GAC) and magnetite was reported...
October 31, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/29095917/comparison-of-the-human-gastric-microbiota-in-hypochlorhydric-states-arising-as-a-result-of-helicobacter-pylori-induced-atrophic-gastritis-autoimmune-atrophic-gastritis-and-proton-pump-inhibitor-use
#9
COMPARATIVE STUDY
Bryony N Parsons, Umer Z Ijaz, Rosalinda D'Amore, Michael D Burkitt, Richard Eccles, Luca Lenzi, Carrie A Duckworth, Andrew R Moore, Laszlo Tiszlavicz, Andrea Varro, Neil Hall, D Mark Pritchard
Several conditions associated with reduced gastric acid secretion confer an altered risk of developing a gastric malignancy. Helicobacter pylori-induced atrophic gastritis predisposes to gastric adenocarcinoma, autoimmune atrophic gastritis is a precursor of type I gastric neuroendocrine tumours, whereas proton pump inhibitor (PPI) use does not affect stomach cancer risk. We hypothesised that each of these conditions was associated with specific alterations in the gastric microbiota and that this influenced subsequent tumour risk...
November 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29094640/stearic-acid-based-systematically-designed-oral-lipid-nanoparticles-for-enhanced-brain-delivery-of-dimethyl-fumarate
#10
Pramod Kumar, Gajanand Sharma, Rajendra Kumar, Ruchi Malik, Bhupinder Singh, Om Prakash Katare, Kaisar Raza
AIM: Dimethyl fumarate is a frequent prescription for the management of numerous neurological disorders. Despite immense promises, DMF is associated with various problems such as multiple dosing (2-3 oral doses daily) and lower brain permeability. Our aim was to enhance the oral bioavailability and increase the brain concentrations of dimethyl fumarate. METHODS: Solid lipid nanoparticles were systematically formulated by optimizing the composition based on the desired attributes viz...
November 2, 2017: Nanomedicine
https://www.readbyqxmd.com/read/29091806/electrochemical-fenton-based-treatment-of-tetracaine-in-synthetic-and-urban-wastewater-using-active-and-non-active-anodes
#11
Carlota Ridruejo, Francesc Centellas, Pere L Cabot, Ignasi Sirés, Enric Brillas
The electrochemical degradation of tetracaine hydrochloride has been studied in urban wastewater. Treatments in simulated matrix with similar ionic composition as well as in 0.050 M Na2SO4 were comparatively performed. The cell contained an air-diffusion cathode for H2O2 electrogeneration and an anode selected among active Pt, IrO2-based and RuO2-based materials and non-active boron-doped diamond (BDD). Electrochemical oxidation with electrogenerated H2O2 (EO-H2O2), electro-Fenton (EF) and photoelectro-Fenton (PEF) were comparatively assessed at pH 3...
October 23, 2017: Water Research
https://www.readbyqxmd.com/read/29075551/development-of-a-lc-ms-ms-method-for-the-simultaneous-detection-of-tricarboxylic-acid-cycle-intermediates-in-a-range-of-biological-matrices
#12
Omar Al Kadhi, Antonietta Melchini, Richard Mithen, Shikha Saha
It is now well-established that perturbations in the tricarboxylic acid (TCA) cycle play an important role in the metabolic transformation occurring in cancer including that of the prostate. A method for simultaneous qualitative and quantitative analysis of TCA cycle intermediates in body fluids, tissues, and cultured cell lines of human origin was developed using a common C18 reversed-phase column by LC-MS/MS technique. This LC-MS/MS method for profiling TCA cycle intermediates offers significant advantages including simple and fast preparation of a wide range of human biological samples...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/29075326/sustainable-carbon-sources-for-microbial-organic-acid-production-with-filamentous-fungi
#13
Stefan Dörsam, Jana Fesseler, Olga Gorte, Thomas Hahn, Susanne Zibek, Christoph Syldatk, Katrin Ochsenreither
BACKGROUND: The organic acid producer Aspergillus oryzae and Rhizopus delemar are able to convert several alternative carbon sources to malic and fumaric acid. Thus, carbohydrate hydrolysates from lignocellulose separation are likely suitable as substrate for organic acid production with these fungi. RESULTS: Before lignocellulose hydrolysate fractions were tested as substrates, experiments with several mono- and disaccharides, possibly present in pretreated biomass, were conducted for their suitability for malic acid production with A...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29069586/fumarate-hydratase-loss-causes-combined-respiratory-chain-defects
#14
Petros A Tyrakis, Marie E Yurkovich, Marco Sciacovelli, Evangelia K Papachristou, Hannah R Bridges, Edoardo Gaude, Alexander Schreiner, Clive D'Santos, Judy Hirst, Juan Hernandez-Fernaud, Roger Springett, John R Griffiths, Christian Frezza
Fumarate hydratase (FH) is an enzyme of the tricarboxylic acid (TCA) cycle mutated in hereditary and sporadic cancers. Despite recent advances in understanding its role in tumorigenesis, the effects of FH loss on mitochondrial metabolism are still unclear. Here, we used mouse and human cell lines to assess mitochondrial function of FH-deficient cells. We found that human and mouse FH-deficient cells exhibit decreased respiration, accompanied by a varying degree of dysfunction of respiratory chain (RC) complex I and II...
October 24, 2017: Cell Reports
https://www.readbyqxmd.com/read/29068516/simultaneous-determination-of-furfural-and-its-degradation-products-furoic-acid-and-maleic-acid-in-transformer-oil-by-the-reversed-phase-vortex-assisted-liquid-liquid-microextraction-followed-by-hplc
#15
Yifan Wang, Haiyan Li, Zhen Yang, Weijie Zhang, Jia Hua
To explore why the use of furfural as a transformer oil-paper insulation aging characteristic is problematic in real world application, we developed a method for the simultaneous determination of furfural, furoic acid and maleic acid in transformer oil by reversed-phase vortex-assisted liquid-liquid microextraction combined with high-performance liquid chromatography. The conditions for the proposed method were optimized, and the obtained extract can be directly analyzed by high-performance liquid chromatography...
October 25, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29068222/stereoselective-deuteration-in-aspartate-asparagine-lysine-and-methionine-amino-acid-residues-using-fumarate-as-a-carbon-source-for-escherichia-coli-in-d2o
#16
Gaddafi I Danmaliki, Philip B Liu, Peter M Hwang
Perdeuteration with selective (1)H,(13)C-enrichment of methyl groups has enabled solution NMR studies of large (>30 kDa) protein systems. However, we propose that for all non-methyl positions, only magnetization originating from (1)H-(12)C groups is sufficiently long-lived, and it can be transferred via through-space NOEs to slowly relaxing (1)H-(15)N or (1)H-(13)C methyl groups to achieve multidimensional solution NMR. We demonstrate stereoselective (1)H,(12)C-labeling by adding relatively inexpensive unlabeled carbon sources to Escherichia coli growth media in D2O...
November 3, 2017: Biochemistry
https://www.readbyqxmd.com/read/29065776/phosphate-functional-groups-improve-opf-osteoconduction-and-bmp-2-osteoinductive-efficacy
#17
Maurits Geert Laurent Olthof, M Tryfonidou, Xifeng Liu, Behdad Pouran, Björn Meij, Wouter Dhert, Michael J Yaszemski, Lichun Lu, Jacqueline Alblas, Diederik H R Kempen
Off the shelf availability in large quantities, drug delivery functionality, and modifiable chemistry and mechanical properties makes synthetic polymers highly suitable candidates for bone grafting. However, most synthetic polymers lack the ability to support cell attachment, proliferation, migration and differentiation and ultimately tissue formation. Incorporating anionic peptides into the polymer that mimic acidic proteins, which contribute to biomineralization and cellular attachment, could enhance bone formation...
October 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29052812/fumarase-deficiency-a-safe-and-potentially-disease-modifying-effect-of-high-fat-low-carbohydrate-diet
#18
B Ryder, F Moore, A Mitchell, S Thompson, J Christodoulou, S Balasubramaniam
Fumarate hydratase deficiency (FHD) caused by biallelic alterations of the FH (fumarate hydratase) gene is a rare disorder of the tricarboxylic acid cycle, classically characterized by encephalopathy, profound psychomotor retardation, seizures, a spectrum of brain abnormalities and early death in childhood. Less common milder phenotypes with moderate cognitive impairment and long-term survival have been reported. In addition, heterozygous mutations of the FH gene are responsible for hereditary leiomyomatosis and renal cell cancer (HLRCC)...
October 21, 2017: JIMD Reports
https://www.readbyqxmd.com/read/29047061/release-and-kinetics-of-arsenic-and-plumbum-in-the-songhua-river-surficial-sediments
#19
Guang-Min Liu, Qing An, Li-Juan Wang, Xin Jia, Si-Hui Feng, Shu-Mei Xia, Yi-Ning Wu, Hong Qi
To estimate the pollution of As and Pb in the Songhua River which flows through the major rice-producing regions in China, the present study investigated the level and release of As and Pb in surficial sediments which collected from nine sites in Songhua River (M1-M9). The concentration of As and Pb was ranged as follows: As = 3.104~15.01 μg/g, Pb = 20.10~37.42 μg/g; the average concentration: As = 6.466 ± 3.077 μg/g, Pb = 28.88 ± 5.077 μg/g. By analysis vertically, the average concentration of As was 5...
October 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29044950/biochemical-characterization-of-argininosuccinate-lyase-from-m-tuberculosis-significance-of-a-c-terminal-cysteine-in-catalysis-and-thermal-stability
#20
Archita Mishra, Avadhesha Surolia
Arginine biosynthesis pathway is crucial to the survival and pathogenesis of Mycobacterium tuberculosis (Mtb). Arginine is a critical amino acid that contributes to the inflection of cellular immune responses during pathogenesis. Argininosuccinate lyase from Mtb (MtArgH), the last enzyme in the pathway, catalyzes the production of arginine from argininosuccinic acid. MtArgH is an essential enzyme for the growth and survival of M. tuberculosis. We biochemically characterized MtArgH and deciphered the role of a previously unexplored cysteine (Cys(441) ) residue at the C-terminal region of the protein...
November 2017: IUBMB Life
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