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https://www.readbyqxmd.com/read/28657161/unambiguous-regiochemical-assignment-of-sulfoquinovosyl-mono-and-di-acylglycerols-in-parsley-and-spinach-leaves-by-liquid-chromatography-electrospray-ionization-sequential-mass-spectrometry-assisted-by-regioselective-enzymatic-hydrolysis
#1
Sara Granafei, Ilario Losito, Francesco Palmisano, Tommaso R I Cataldi
RATIONALE: Sulfoquinovosylmonoglycerides (SQMG) and sulfoquinovosyldiglycerides (SQDG) in the lipid extracts of parsley (Petroselinum crispum) and spinach (Spinacia oleracea) leaves were investigated. The aim of this work was to assess and establish the chemical characterization of fatty acyl chains in sulfolipids (SQMG and SQDG) and their regiochemistry. METHODS: A key component of this approach is a combination of hydrolysis reactions catalyzed by Lecitase® Ultra, which is a sn1 -regioselective hydrolase enzyme, and reversed-phase liquid chromatography with electrospray ionization and sequential mass spectrometry (RPLC-ESI-MS) by collision induced dissociation (CID)-MS(n) (n=2,3)...
June 28, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28646670/a-metagenomic-assessment-of-viral-contamination-on-fresh-parsley-plants-irrigated-with-fecally-tainted-river-water
#2
X Fernandez-Cassi, N Timoneda, E Gonzales-Gustavson, J F Abril, S Bofill-Mas, R Girones
Microbial food-borne diseases are still frequently reported despite the implementation of microbial quality legislation to improve food safety. Among all the microbial agents, viruses are the most important causative agents of food-borne outbreaks. The development and application of a new generation of sequencing techniques to test for viral contaminants in fresh produce is an unexplored field that allows for the study of the viral populations that might be transmitted by the fecal-oral route through the consumption of contaminated food...
June 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28610170/integration-of-molecular-bioacoustical-and-morphological-data-reveals-two-new-cryptic-species-of-i-pelodytes-i-anura-pelodytidae-from-the-iberian-peninsula
#3
Jesús Díaz-Rodríguez, Marcelo Gehara, Rafael Márquez, Miguel Vences, Helena Gonçalves, Fernando Sequeira, Iñigo Martínez-Solano, Miguel Tejedo
Parsley frogs (Pelodytes) comprise the only genus in the family Pelodytidae, an ancient anuran lineage that split from their closest relatives over 140 million years ago. Pelodytes is a Palearctic group restricted to Western Eurasia including three extant species: the eastern species P. caucasicus, endemic to the Caucasus area, and two closely related species inhabiting Western Europe: the Iberian endemic P. ibericus and the more widespread P. punctatus. Previous studies based on mitochondrial and nuclear DNA markers have revealed the existence of two additional lineages of Parsley frogs in the Iberian Peninsula, which have been flagged as candidate species...
March 13, 2017: Zootaxa
https://www.readbyqxmd.com/read/28603396/accessing-good-health-information-and-resources
#4
Sally Parsley
No abstract text is available yet for this article.
2017: Community Eye Health
https://www.readbyqxmd.com/read/28581738/identification-and-quantitation-of-furocoumarins-in-popularly-consumed-foods-in-the-u-s-using-quechers-extraction-coupled-with-uplc-ms-ms-analysis
#5
Melissa M Melough, Sang Gil Lee, Eunyoung Cho, Kijoon Kim, Anthony A Provatas, Christopher Perkins, Min Kyung Park, Abrar Qureshi, Ock K Chun
Furocoumarins are a class of photoactive compounds found in several plant species and may be responsible for the observed association between consumption of citrus products and the risk of skin cancer. Furocoumarin contents of several foods have been reported previously, but no comprehensive database of furocoumarin content of foods is currently available. Therefore, this study aimed to determine the distribution of furocoumarins in popularly consumed foods in the U.S. Samples of three varieties of each of 29 foods known or suspected to contain furocoumarins were purchased, prepared for analysis using a solid phase extraction method, and analyzed using UPLC-MS/MS for the presence of seven major furocoumarins...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28580843/risk-assessment-of-combined-exposure-to-alkenylbenzenes-through-consumption-of-plant-food-supplements-containing-parsley-and-dill
#6
Abdalmajeed M Alajlouni, Amer J Al-Malahmeh, Sebastiaan Wesseling, Marina Kalli, Jacques Vervoort, Ivonne M C M Rietjens
A risk assessment was performed of parsley and dill based plant food supplements (PFS) containing apiol and related alkenylbenzenes. First the levels of the alkenylbenzenes in the PFS and the resulting estimated daily intake (EDI) resulting from use of the PFS were quantified. Since most PFS appeared to contain more than one alkenylbenzene a combined risk assessment was performed based on equal potency or using a so-called Toxic Equivalency (TEQ) approach based on Toxic Equivalency Factors (TEFs) for the different alkenylbenzenes...
June 5, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28567399/bicruciate-retaining-total-knee-arthroplasty-a-promising-technology-that-s-not-quite-there
#7
EDITORIAL
Thomas J Barrett, Lei Shi, Brian S Parsley
No abstract text is available yet for this article.
May 2017: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28538283/beans-cilantro-and-parsley-3-unadvertised-though-relevant-calcium-food-sources
#8
Rodolfo de Melo Nunes, Virgínia Claudia Carneiro Girão, Francisco Airton Castro Rocha
No abstract text is available yet for this article.
June 2017: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/28527368/effects-of-parsley-extract-powder-as-an-alternative-for-the-direct-addition-of-sodium-nitrite-in-the-production-of-mortadella-type-sausages-impact-on-microbiological-physicochemical-and-sensory-aspects
#9
Greta Riel, Annika Boulaaba, Johanna Popp, Guenter Klein
Increasing concern about chemical additives in processed meat has led to an increased market of uncured and alternatively cured meat products. However, the use of vegetable extracts or the exclusion of curing salt may increase the risk of greater bacterial growth and alteration of several physicochemical parameters. Therefore, in this study mortadella-type sausages, manufactured with 1.07 (V3), 2.14 (V4) and 4.29 (V5) g parsley extract powder/kg sausage meat were produced. These sausage variants were compared to an uncured (V2) and a traditionally nitrite-cured control (V1)...
May 12, 2017: Meat Science
https://www.readbyqxmd.com/read/28506462/a-slow-conformational-switch-in-the-bmal1-transactivation-domain-modulates-circadian-rhythms
#10
Chelsea L Gustafson, Nicole C Parsley, Hande Asimgil, Hsiau-Wei Lee, Christopher Ahlbach, Alicia K Michael, Haiyan Xu, Owen L Williams, Tara L Davis, Andrew C Liu, Carrie L Partch
The C-terminal transactivation domain (TAD) of BMAL1 (brain and muscle ARNT-like 1) is a regulatory hub for transcriptional coactivators and repressors that compete for binding and, consequently, contributes to period determination of the mammalian circadian clock. Here, we report the discovery of two distinct conformational states that slowly exchange within the dynamic TAD to control timing. This binary switch results from cis/trans isomerization about a highly conserved Trp-Pro imide bond in a region of the TAD that is required for normal circadian timekeeping...
May 18, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28460944/the-transfer-rate-of-salmonella-typhimurium-from-contaminated-parsley-to-other-consecutively-chopped-batches-via-cutting-boards-under-different-food-handling-scenarios
#11
Dima Faour-Klingbeil, Victor Kuri, Ewen Todd
In many Mediterranean and Middle Eastern countries, leafy green parsley is typically eaten raw and prepared by fine chopping several batches. This study aimed to quantify the transfer rate of S. Typhimurium (Tr) across all chopped batches in scenarios that resemble normally occurring operations in restaurants and home kitchens. Fresh parsley leaves were inoculated at concentrations of 6 and 3logCFU/g and chopped on a polyethylene cutting board (CB). Uninoculated parsleys were sequentially chopped in individual batches on the same cutting surface, 1) instantly (CB Instant), 2) after washing in water and holding at 30°C 24h (CBWW), 3) after washing in soapy water, sponge scrubbing and holding at 30°C 24h (CB SW)...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28455528/flavonoidsearch-a-system-for-comprehensive-flavonoid-annotation-by-mass-spectrometry
#12
Nayumi Akimoto, Takeshi Ara, Daisuke Nakajima, Kunihiro Suda, Chiaki Ikeda, Shingo Takahashi, Reiko Muneto, Manabu Yamada, Hideyuki Suzuki, Daisuke Shibata, Nozomu Sakurai
Currently, in mass spectrometry-based metabolomics, limited reference mass spectra are available for flavonoid identification. In the present study, a database of probable mass fragments for 6,867 known flavonoids (FsDatabase) was manually constructed based on new structure- and fragmentation-related rules using new heuristics to overcome flavonoid complexity. We developed the FlavonoidSearch system for flavonoid annotation, which consists of the FsDatabase and a computational tool (FsTool) to automatically search the FsDatabase using the mass spectra of metabolite peaks as queries...
April 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28444161/odor-perception-by-dogs-evaluating-two-training-approaches-for-odor-learning-of-sniffer-dogs
#13
Carola Fischer-Tenhagen, Dorothea Johnen, Wolfgang Heuwieser, Roland Becker, Kristin Schallschmidt, Irene Nehls
In this study, a standardized experimental set-up with various combinations of herbs as odor sources was designed. Two training approaches for sniffer dogs were compared; first, training with a pure reference odor, and second, training with a variety of odor mixtures with the target odor as a common denominator. The ability of the dogs to identify the target odor in a new context was tested. Six different herbs (basil, St. John's wort, dandelion, marjoram, parsley, ribwort) were chosen to produce reference materials in various mixtures with (positive) and without (negative) chamomile as the target odor source...
June 1, 2017: Chemical Senses
https://www.readbyqxmd.com/read/28441391/apigenin-inhibits-tnf%C3%AE-il-1%C3%AE-induced-ccl2-release-through-ikbk-epsilon-signaling-in-mda-mb-231-human-breast-cancer-cells
#14
David Bauer, Natalie Redmon, Elizabeth Mazzio, Karam F Soliman
Mortality associated with breast cancer is attributable to aggressive metastasis, to which TNFα plays a central orchestrating role. TNFα acts on breast tumor TNF receptors evoking the release of chemotactic proteins (e.g. MCP-1/CCL2). These proteins direct inward infiltration/migration of tumor-associated macrophages (TAMs), tumor-associated neutrophils (TANs), myeloid-derived suppressor cells (MDSCs), T-regulatory cells (Tregs), T helper IL-17-producing cells (Th17s), metastasis-associated macrophages (MAMs) and cancer-associated fibroblasts (CAFs)...
2017: PloS One
https://www.readbyqxmd.com/read/28400115/thymus-transplantation-for-complete-digeorge-syndrome-european-experience
#15
E Graham Davies, Melissa Cheung, Kimberly Gilmour, Jesmeen Maimaris, Joe Curry, Anna Furmanski, Neil Sebire, Neil Halliday, Konstantinos Mengrelis, Stuart Adams, Jolanta Bernatoniene, Ronald Bremner, Michael Browning, Blythe Devlin, Hans Christian Erichsen, H Bobby Gaspar, Lizzie Hutchison, Winnie Ip, Marianne Ifversen, T Ronan Leahy, Elizabeth McCarthy, Despina Moshous, Kim Neuling, Malgorzata Pac, Alina Papadopol, Kathryn L Parsley, Luigi Poliani, Ida Ricciardelli, David M Sansom, Tiia Voor, Austen Worth, Tessa Crompton, M Louise Markert, Adrian J Thrasher
BACKGROUND: Thymus transplantation is a promising strategy for the treatment of athymic complete DiGeorge syndrome (cDGS). METHODS: Twelve patients with cDGS were transplanted with allogeneic cultured thymus. OBJECTIVE: To confirm and extend the results previously obtained in a single centre. RESULTS: Two patients died of pre-existing viral infections without developing thymopoeisis and one late death occurred from autoimmune thrombocytopaenia...
April 8, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28392421/phenylalanine-ammonia-lyase-from-arabidopsis-thaliana-atpal2-a-potent-enzyme-for-the-synthesis-of-non-canonical-aromatic-alpha-amino-acids-part-i-comparative-characterization-to-the-enzymes-from-petroselinum-crispum-pcpal1-and-rhodosporidium-toruloides-rtpal
#16
Alana Dreßen, Thomas Hilberath, Ursula Mackfeld, Arne Billmeier, Jens Rudat, Martina Pohl
Phenylalanine ammonia lyase (PAL) from Arabidopsis thaliana (AtPAL2) was comparatively characterized to the well-studied enzyme from parsley (PcPAL1) and Rhodosporidium toruloides (RtPAL) with respect to kinetic parameters for the deamination and the amination reaction, pH- and temperature optima and the substrate range of the amination reaction. Whereas both plant enzymes are specific for phenylalanine, the bifunctional enzyme from Rhodosporidium toruloides shows KM-values for L-Phe and L-Tyr in the same order of magnitude and, compared to both plant enzymes, a 10-15-fold higher activity...
April 6, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28376348/cadmium-in-edible-plants-from-silesia-poland-and-its-implications-for-health-risk-in-populations
#17
Grzegorz Dziubanek, Renata Baranowska, Małgorzata Ćwieląg-Drabek, Anna Spychała, Agata Piekut, Monika Rusin, Ilona Hajok
The health risk associated with cadmium exposure through consumption of edible plants cultivated in different parts of the Silesia administration area inhabitants has been estimated. The impact of the arable soils acidity on the BCF (bioconcentration factor) has been also analyzed. The concentration of cadmium in arable soils and in 118 samples of vegetables - carrots, beets, potato, celery (tuber), parsley (root) were determined. The cadmium content in examined soil samples were in the range from >0.5-68...
August 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28345998/pharmacokinetics-of-a-single-dose-of-voriconazole-administered-orally-with-and-without-food-to-red-tailed-hawks-buteo-jamaicensus
#18
RANDOMIZED CONTROLLED TRIAL
Ruth A Parsley, Lisa A Tell, Ronette Gehring
OBJECTIVE To determine the pharmacokinetics of voriconazole administered PO with or without food to red-tailed hawks (Buteo jamaicensus) and whether any observed variability could be explained by measured covariates to inform dose adjustments. ANIMALS 7 adult red-tailed hawks. PROCEDURES In a crossover study design, hawks were randomly assigned to first receive voriconazole (15 mg/kg, PO) injected into a dead mouse (n = 3; fed birds) or without food (4; unfed birds). Sixteen days later, treatments were reversed...
April 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28341984/combinatorial-requirement-of-w-and-wt-boxes-in-microbe-associated-molecular-pattern-responsive-synthetic-promoters
#19
Konstantin Kanofsky, Ann-Kathrin Bahlmann, Reinhard Hehl, Do Xuan Dong
The WT-box GGACTTTC belongs to a novel class of MAMP-responsive cis-regulatory sequences that are part of combinatorial elements. Microbe-associated molecular pattern (MAMP)-responsive synthetic promoters were generated with two cis-regulatory modules (CRM1 and CRM2) from the Arabidopsis thaliana WRKY30 promoter. Both modules harbour two W-boxes and one WT-box. Mutation analysis of the synthetic promoters and transient gene expression analysis in parsley protoplasts underline the importance of the W- and WT-boxes for MAMP-responsive gene expression and reveal the combinatorial requirement of at least two boxes for full MAMP responsivity...
March 24, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28317756/blue-light-dosage-affects-carotenoids-and-tocopherols-in-microgreens
#20
Giedrė Samuolienė, Akvilė Viršilė, Aušra Brazaitytė, Julė Jankauskienė, Sandra Sakalauskienė, Viktorija Vaštakaitė, Algirdas Novičkovas, Alina Viškelienė, Audrius Sasnauskas, Pavelas Duchovskis
Mustard, beet and parsley were grown to harvest time under selected LEDs: 638+660+731+0% 445nm; 638+660+731+8% 445nm; 638+660+731+16% 445nm; 638+660+731+25% 445nm; 638+660+731+33% 445nm. From 1.2 to 4.3 times higher concentrations of chlorophylls a and b, carotenoids, α- and β-carotenes, lutein, violaxanthin and zeaxanthin was found under blue 33% treatment in comparison to lower blue light dosages. Meanwhile, the accumulation of metabolites, which were not directly connected with light reactions, such as tocopherols, was more influenced by lower (16%) blue light dosage, increasing about 1...
August 1, 2017: Food Chemistry
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