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https://www.readbyqxmd.com/read/28444161/odor-perception-by-dogs-evaluating-two-training-approaches-for-odor-learning-of-sniffer-dogs
#1
Carola Fischer-Tenhagen, Dorothea Johnen, Wolfgang Heuwieser, Roland Becker, Kristin Schallschmidt, Irene Nehls
In this study, a standardized experimental set-up with various combinations of herbs as odor sources was designed. Two training approaches for sniffer dogs were compared; first, training with a pure reference odor, and second, training with a variety of odor mixtures with the target odor as a common denominator. The ability of the dogs to identify the target odor in a new context was tested. Six different herbs (basil, St. John's wort, dandelion, marjoram, parsley, ribwort) were chosen to produce reference materials in various mixtures with (positive) and without (negative) chamomile as the target odor source...
April 21, 2017: Chemical Senses
https://www.readbyqxmd.com/read/28441391/apigenin-inhibits-tnf%C3%AE-il-1%C3%AE-induced-ccl2-release-through-ikbk-epsilon-signaling-in-mda-mb-231-human-breast-cancer-cells
#2
David Bauer, Natalie Redmon, Elizabeth Mazzio, Karam F Soliman
Mortality associated with breast cancer is attributable to aggressive metastasis, to which TNFα plays a central orchestrating role. TNFα acts on breast tumor TNF receptors evoking the release of chemotactic proteins (e.g. MCP-1/CCL2). These proteins direct inward infiltration/migration of tumor-associated macrophages (TAMs), tumor-associated neutrophils (TANs), myeloid-derived suppressor cells (MDSCs), T-regulatory cells (Tregs), T helper IL-17-producing cells (Th17s), metastasis-associated macrophages (MAMs) and cancer-associated fibroblasts (CAFs)...
2017: PloS One
https://www.readbyqxmd.com/read/28400115/thymus-transplantation-for-complete-digeorge-syndrome-european-experience
#3
E Graham Davies, Melissa Cheung, Kimberly Gilmour, Jesmeen Maimaris, Joe Curry, Anna Furmanski, Neil Sebire, Neil Halliday, Konstantinos Mengrelis, Stuart Adams, Jolanta Bernatoniene, Ronald Bremner, Michael Browning, Blythe Devlin, Hans Christian Erichsen, H Bobby Gaspar, Lizzie Hutchison, Winnie Ip, Marianne Ifversen, T Ronan Leahy, Elizabeth McCarthy, Despina Moshous, Kim Neuling, Malgorzata Pac, Alina Papadopol, Kathryn L Parsley, Luigi Poliani, Ida Ricciardelli, David M Sansom, Tiia Voor, Austen Worth, Tessa Crompton, M Louise Markert, Adrian J Thrasher
BACKGROUND: Thymus transplantation is a promising strategy for the treatment of athymic complete DiGeorge syndrome (cDGS). METHODS: Twelve patients with cDGS were transplanted with allogeneic cultured thymus. OBJECTIVE: To confirm and extend the results previously obtained in a single centre. RESULTS: Two patients died of pre-existing viral infections without developing thymopoeisis and one late death occurred from autoimmune thrombocytopaenia...
April 8, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28392421/phenylalanine-ammonia-lyase-from-arabidopsis-thaliana-atpal2-a-potent-enzyme-for-the-synthesis-of-non-canonical-aromatic-alpha-amino-acids-part-i-comparative-characterization-to-the-enzymes-from-petroselinum-crispum-pcpal1-and-rhodosporidium-toruloides-rtpal
#4
Alana Dreßen, Thomas Hilberath, Ursula Mackfeld, Arne Billmeier, Jens Rudat, Martina Pohl
Phenylalanine ammonia lyase (PAL) from Arabidopsis thaliana (AtPAL2) was comparatively characterized to the well-studied enzyme from parsley (PcPAL1) and Rhodosporidium toruloides (RtPAL) with respect to kinetic parameters for the deamination and the amination reaction, pH- and temperature optima and the substrate range of the amination reaction. Whereas both plant enzymes are specific for phenylalanine, the bifunctional enzyme from Rhodosporidium toruloides shows KM-values for L-Phe and L-Tyr in the same order of magnitude and, compared to both plant enzymes, a 10-15-fold higher activity...
April 6, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28376348/cadmium-in-edible-plants-from-silesia-poland-and-its-implications-for-health-risk-in-populations
#5
Grzegorz Dziubanek, Renata Baranowska, Małgorzata Ćwieląg-Drabek, Anna Spychała, Agata Piekut, Monika Rusin, Ilona Hajok
The health risk associated with cadmium exposure through consumption of edible plants cultivated in different parts of the Silesia administration area inhabitants has been estimated. The impact of the arable soils acidity on the BCF (bioconcentration factor) has been also analyzed. The concentration of cadmium in arable soils and in 118 samples of vegetables - carrots, beets, potato, celery (tuber), parsley (root) were determined. The cadmium content in examined soil samples were in the range from >0.5-68...
April 1, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28345998/pharmacokinetics-of-a-single-dose-of-voriconazole-administered-orally-with-and-without-food-to-red-tailed-hawks-buteo-jamaicensus
#6
Ruth A Parsley, Lisa A Tell, Ronette Gehring
OBJECTIVE To determine the pharmacokinetics of voriconazole administered PO with or without food to red-tailed hawks (Buteo jamaicensus) and whether any observed variability could be explained by measured covariates to inform dose adjustments. ANIMALS 7 adult red-tailed hawks. PROCEDURES In a crossover study design, hawks were randomly assigned to first receive voriconazole (15 mg/kg, PO) injected into a dead mouse (n = 3; fed birds) or without food (4; unfed birds). Sixteen days later, treatments were reversed...
April 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28341984/combinatorial-requirement-of-w-and-wt-boxes-in-microbe-associated-molecular-pattern-responsive-synthetic-promoters
#7
Konstantin Kanofsky, Ann-Kathrin Bahlmann, Reinhard Hehl, Do Xuan Dong
The WT-box GGACTTTC belongs to a novel class of MAMP-responsive cis-regulatory sequences that are part of combinatorial elements. Microbe-associated molecular pattern (MAMP)-responsive synthetic promoters were generated with two cis-regulatory modules (CRM1 and CRM2) from the Arabidopsis thaliana WRKY30 promoter. Both modules harbour two W-boxes and one WT-box. Mutation analysis of the synthetic promoters and transient gene expression analysis in parsley protoplasts underline the importance of the W- and WT-boxes for MAMP-responsive gene expression and reveal the combinatorial requirement of at least two boxes for full MAMP responsivity...
March 24, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28317756/blue-light-dosage-affects-carotenoids-and-tocopherols-in-microgreens
#8
Giedrė Samuolienė, Akvilė Viršilė, Aušra Brazaitytė, Julė Jankauskienė, Sandra Sakalauskienė, Viktorija Vaštakaitė, Algirdas Novičkovas, Alina Viškelienė, Audrius Sasnauskas, Pavelas Duchovskis
Mustard, beet and parsley were grown to harvest time under selected LEDs: 638+660+731+0% 445nm; 638+660+731+8% 445nm; 638+660+731+16% 445nm; 638+660+731+25% 445nm; 638+660+731+33% 445nm. From 1.2 to 4.3 times higher concentrations of chlorophylls a and b, carotenoids, α- and β-carotenes, lutein, violaxanthin and zeaxanthin was found under blue 33% treatment in comparison to lower blue light dosages. Meanwhile, the accumulation of metabolites, which were not directly connected with light reactions, such as tocopherols, was more influenced by lower (16%) blue light dosage, increasing about 1...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28290475/the-development-of-loop-mediated-isothermal-amplification-lamp-assays-for-the-rapid-authentication-of-five-forbidden-vegetables-in-strict-vegetarian-diets
#9
Meng-Shiou Lee, Ting-Ying Su, Yi-Yang Lien, Shyang-Chwen Sheu
Plant-based food ingredients such as garlic, Chinese leek, Chinese onion, green onion and onion are widely used in many cuisines around the world. However, these ingredients known as the "five forbidden vegetables" (FFVs) are not allowed in some vegetarian diets. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of FFVs using five respective LAMP primer sets. The specific primers targeted the ITS1-5.8S-ITS2 nuclear ribosomal DNA sequence regions among the five vegetables...
March 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28260107/cytoprotective-effects-of-myristicin-against-hypoxia%C3%A2-induced-apoptosis-and-endoplasmic-reticulum-stress-in-rat-dorsal-root-ganglion-neurons
#10
Quanlai Zhao, Chen Liu, Xiang Shen, Liang Xiao, Hong Wang, Ping Liu, Lingting Wang, Hongguang Xu
The aim of the present study was to investigate the role of myristicin (Myr; 1‑allyl‑5‑methoxy‑3,4‑methylenedioxybenzene), an active aromatic compound isolated from nutmeg, carrot, basil, cinnamon and parsley, in hypoxia‑induced apoptosis in rat dorsal root ganglion (DRG) neurons. It was observed that Myr significantly enhanced cell viability in hypoxia‑induced DRG neurons in a dose‑dependent manner; the optimal concentration of Myr was 50 µM. Furthermore, Myr reduced the percentage of deoxynucleotidyl transferase‑mediated dUTP nick end‑labeling‑positive neuronal cells and influenced the expression of the pro‑apoptotic gene B‑cell lymphoma 2 (Bcl‑2) associated X protein, the apoptosis protease cleaved caspase‑3 and the anti‑apoptotic gene Bcl‑2, in the hypoxia‑induced group...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28254010/preharvest-treatments-with-malic-oxalic-and-acetylsalicylic-acids-affect-the-phenolic-composition-and-antioxidant-capacity-of-coriander-dill-and-parsley
#11
Hussein El-Zaeddi, Ángel Calín-Sánchez, Paulina Nowicka, Juan Martínez-Tomé, Luis Noguera-Artiaga, Francisco Burló, Aneta Wojdyło, Ángel A Carbonell-Barrachina
The effects of a preharvest treatment with malic (MA), oxalic (OA), or acetylsalicylic (ASA) acid at three concentrations (1, 2 and 3mM) on the bioactivity and antioxidant capacity of coriander, dill, and parsley were investigated. The antioxidant capacity of the herbs extracts was assayed by spectrophotometric methods by using three different analytical methods: ORAC, FRAP, and ABTS; the effects of treatments were very positive in coriander, produced intermediate results in dill, and no effects were found in parsley plants...
July 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28251024/petroselinum-crispum-is-effective-in-reducing-stress-induced-gastric-oxidative-damage
#12
Ayşin Akıncı, Mukaddes Eşrefoğlu, Elif Taşlıdere, Burhan Ateş
BACKGROUND: Oxidative stress has been shown to play a principal role in the pathogenesis of stress-induced gastric injury. Parsley (Petroselinum crispum) contains many antioxidants such as flavanoids, carotenoids and ascorbic acid. AIMS: In this study, the histopathological and biochemical results of nutrition with a parsley-rich diet in terms of eliminating stress-induced oxidative gastric injury were evaluated. STUDY DESIGN: Animal experimentation...
January 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28231136/volatile-composition-of-essential-oils-from-different-aromatic-herbs-grown-in-mediterranean-regions-of-spain
#13
Hussein El-Zaeddi, Juan Martínez-Tomé, Ángel Calín-Sánchez, Francisco Burló, Ángel A Carbonell-Barrachina
Volatile composition of essential oils from dill, parsley, coriander, and mint were investigated at different harvest dates to determine the most suitable harvest time for each these herbs. Hydrodistillation (HD), using a Deryng system, was used for isolating the essential oils. Isolation and identification of the volatile compounds were performed using gas chromatography-mass spectrometry (GC-MS) instrument. The results of gas chromatography-flame ionization detector (GC-FID) analysis (quantification) showed that the main components in the essential oil of dill shoots were α-phellandrene, dill ether, and β-phellandrene, and the optimal harvest date was D2 (second harvest, fourth week of February 2015)...
May 25, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28231113/chemical-composition-antioxidant-and-antimicrobial-activity-of-essential-oils-from-organic-fennel-parsley-and-lavender-from-spain
#14
Irene Marín, Estrella Sayas-Barberá, Manuel Viuda-Martos, Casilda Navarro, Esther Sendra
The aim of this work was to (i) determine the chemical composition of the essential oils of three spices widely cultivated in Spain from organic growth: Foeniculum vulgare, Petroselium crispum, and Lavandula officinalis; (ii) determine the total phenolic content; (iii) determine the antioxidant activity of the essentials oils by means of three different antioxidant tests and (iv) determine the effectiveness of these essentials oils on the inhibition of Listeria innocua CECT 910 and Pseudomonas fluorescens CECT 844...
March 4, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28224908/comparison-of-multivariate-curve-resolution-strategies-in-quantitative-lcxlc-application-to-the-quantification-of-furanocoumarins-in-apiaceous-vegetables
#15
Daniel W Cook, Mackenzie L Burnham, David C Harmes, Dwight R Stoll, Sarah C Rutan
Comprehensive two-dimensional liquid chromatography (LC × LC) has been gaining popularity for the analysis of complex samples in a wide range of fields including metabolomics, environmental analysis, and food analysis. While LC × LC can provide greater chromatographic resolution than one-dimensional LC (1D-LC), overlapping peaks are often still present in separations of complex samples, a problem that can be alleviated by chemometric curve resolution techniques such as multivariate curve resolution-alternating least squares (MCR-ALS)...
April 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28193018/thin-layer-chromatography-desorption-atmospheric-pressure-photoionization-orbitrap-mass-spectrometry-of-lipids
#16
Jan Rejšek, Vladimír Vrkoslav, Anu Vaikkinen, Markus Haapala, Tiina J Kauppila, Risto Kostiainen, Josef Cvačka
Desorption atmospheric pressure photoionization (DAPPI) allows surface analysis in the open atmosphere and is thus an appropriate method for the direct coupling of thin-layer chromatography (TLC) and mass spectrometry (MS). Here, the capability of DAPPI-MS for ionizing and detecting lipids, namely, cholesterol, triacylglycerols, 1,2-diol diesters, wax esters, cholesteryl esters, and hydrocarbons, from TLC and high-performance thin-layer chromatography (HPTLC) plates in MS and MS(2) modes was tested. Limits of detection for lipid standards separated using normal-phase (NP)-TLC and NP-HPTLC were established...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28092761/chlorinated-and-ultraviolet-radiation-treated-reclaimed-irrigation-water-is-the-source-of-aeromonas-found-in-vegetables-used-for-human-consumption
#17
Fadua Latif-Eugenín, Roxana Beaz-Hidalgo, Carolina Silvera-Simón, Xavi Fernandez-Cassi, María J Figueras
Wastewater is increasingly being recognized as a key water resource, and reclaimed water (or treated wastewater) is used for irrigating vegetables destined for human consumption. The aim of the present study was to determine the diversity and prevalence of Aeromonas spp. both in reclaimed water used for irrigation and in the three types of vegetables irrigated with that water. Seven of the 11 (63.6%) samples of reclaimed water and all samples of vegetables were positive for the presence of Aeromonas. A total of 216 Aeromonas isolates were genotyped and corresponded to 132 different strains that after identification by sequencing the rpoD gene belonged to 10 different species...
April 2017: Environmental Research
https://www.readbyqxmd.com/read/28053955/role-of-apigenin-in-cancer-prevention-via-the-induction-of-apoptosis-and-autophagy
#18
REVIEW
Bokyung Sung, Hae Young Chung, Nam Deuk Kim
Apigenin (4',5,7-trihydroxyflavone) is a flavonoid commonly found in many fruits and vegetables such as parsley, chamomile, celery, and kumquats. In the last few decades, recognition of apigenin as a cancer chemopreventive agent has increased. Significant progress has been made in studying the chemopreventive aspects of apigenin both in vitro and in vivo. Several studies have demonstrated that the anticarcinogenic properties of apigenin occur through regulation of cellular response to oxidative stress and DNA damage, suppression of inflammation and angiogenesis, retardation of cell proliferation, and induction of autophagy and apoptosis...
December 2016: Journal of Cancer Prevention
https://www.readbyqxmd.com/read/28049122/generation-of-gene-edited-chrysanthemum-morifolium-using-multi-copy-transgenes-as-targets-and-markers
#19
Mitsuko Kishi-Kaboshi, Ryutaro Aida, Katsutomo Sasaki
The most widely used gene editing technology-the CRISPR/Cas9 system-employs a bacterial monomeric DNA endonuclease known as clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) and single-guide RNA (sgRNA) that directs Cas9 to a complementary target DNA. However, introducing mutations into higher polyploid plant species, especially for species without genome information, has been difficult. Chrysanthemum morifolium (chrysanthemum) is one of the most important ornamental plants, but it is a hexaploid with a large genome; moreover, it lacks whole genome information...
January 3, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28040159/relative-effectiveness-of-selected-preenrichment-media-for-the-detection-of-salmonella-from-leafy-green-produce-and-herbs
#20
Andrew P Jacobson, Hua Wang, Vikas S Gill, Robert Duvall, Gabriela Arce, Stuart Chirtel, Thomas S Hammack
Four buffered preenrichment media (BAX(®) System MP Media (BAX)), Universal Preenrichment Broth (UPB), modified Buffered Peptone Water (mBPW), and Buffered Peptone Water (BPW)) were compared with lactose broth (LB) in the Bacteriological Analytical Manual's (BAM) Salmonella culture method for the analysis of 9 leafy green produce and herb types. Artificially contaminated test portions were pre-enriched in each medium and the results were analyzed statistically using Fisher's Exact 2-tailed F test (p < 0...
May 2017: Food Microbiology
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