keyword
MENU ▼
Read by QxMD icon Read
search

isoleucine

keyword
https://www.readbyqxmd.com/read/27933294/branched-chain-amino-acid-levels-are-related-with-surrogates-of-disturbed-lipid-metabolism-among-older-men
#1
Urho M Kujala, Markku Peltonen, Merja K Laine, Jaakko Kaprio, Olli J Heinonen, Jouko Sundvall, Johan G Eriksson, Antti Jula, Seppo Sarna, Heikki Kainulainen
AIMS/HYPOTHESIS: Existing studies suggest that decreased branched-chain amino acid (BCAA) catabolism and thus elevated levels in blood are associated with metabolic disturbances. Based on such information, we have developed a hypothesis how BCAA degradation mechanistically connects to tricarboxylic acid cycle, intramyocellular lipid storage, and oxidation, thus allowing more efficient mitochondrial energy production from lipids as well as providing better metabolic health. We analyzed whether data from aged Finnish men are in line with our mechanistic hypothesis linking BCAA catabolism and metabolic disturbances...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27928777/clinical-and-mutational-characterizations-of-ten-indian-patients-with-beta-ketothiolase-deficiency
#2
Elsayed Abdelkreem, Radha Rama Devi Akella, Usha Dave, Sudhir Sane, Hiroki Otsuka, Hideo Sasai, Yuka Aoyama, Mina Nakama, Hidenori Ohnishi, Shaimaa Mahmoud, Mohamed Abd El Aal, Toshiyuki Fukao
Beta-ketothiolase deficiency (mitochondrial acetoacetyl-CoA thiolase (T2) deficiency) is an inherited disease of isoleucine catabolism and ketone body utilization caused by ACAT1 mutations. We identified ten Indian patients who manifested with ketoacidotic episodes of variable severity. The patients showed increased urinary excretion of isoleucine-catabolic intermediates: 2-methyl-3-hydroxybutyrate, 2-methylacetoacetate, and tiglylglycine. Six patients had a favorable outcome, one died, and three developed neurodevelopmental sequela...
December 8, 2016: JIMD Reports
https://www.readbyqxmd.com/read/27924129/interplay-of-genes-in-plant-pathogen-interactions-in-planta-expression-and-docking-studies-of-a-beta-1-3-glucanase-gene-from-piper-colubrinum-and-a-glucanase-inhibitor-gene-from-phytophthora-capsici
#3
K Johnson George, O Rosana Babu, I P Vijesh Kumar, J Santhosh Eapen, M Anandaraj
Oomycete pathogen, Phytophthora capsici is devastating for black pepper (Piper nigrum L.) and causes foot rot disease at all stages of plant growth. Phytophthora secretes a glucanase inhibitor protein (GIP), which is capable of inhibiting defence proteins like endoglucanases. In this particular study Quantitative PCR analysis, molecular docking studies and analysis of sequences of Glucanase inhibitor protein and beta-1,3 glucanse genes were done mainly depending on the data derived from Phytophthora capsici whole genome sequencing and Piper colubrinum RNA-sequencing (RNA-Seq)...
October 2016: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/27920791/extending-a-single-residue-switch-for-abbreviating-catalysis-in-plant-ent-kaurene-synthases
#4
Meirong Jia, Reuben J Peters
Production of ent-kaurene as a precursor for important signaling molecules such as the gibberellins seems to have arisen early in plant evolution, with corresponding cyclase(s) present in all land plants (i.e., embryophyta). The relevant enzymes seem to represent fusion of the class II diterpene cyclase that produces the intermediate ent-copalyl diphosphate (ent-CPP) and the subsequently acting class I diterpene synthase that produces ent-kaurene, although the bifunctionality of the ancestral gene is only retained in certain early diverging plants, with gene duplication and sub-functionalization leading to distinct ent-CPP synthases and ent-kaurene synthases (KSs) generally observed...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27917847/poultry-products-enriched-with-nutricines-have-beneficial-effects-on-human-health
#5
Gordana Kralik, Zlata Kralik
The paper presents nutritive value of broiler meat and table eggs, as these animal products are used for human nutrition on a daily basis. In the Republic of Croatia, average consumption of poultry meat amounts to 18.3 kg and to 160 eggs per capita.The most quality parts of broiler carcass are breasts and drumsticks with thighs. Breast muscles contain 21-23% protein, 1.90-1.97% fat, 75.28-76.01% water and 0.74-0.77% collagen. Thigh muscles contain 4.70-6.05% fat, 19.03-19.93% protein and 0.91-1.13% collagen...
February 1, 2017: Medicinski Glasnik
https://www.readbyqxmd.com/read/27914015/electron-transfer-dissociation-of-all-ions-at-all-times-ms-etd-in-a-quadrupole-time-of-flight-q-tof-mass-spectrometer
#6
Christian N Cramer, Jeffery M Brown, Nick Tomczyk, Peter Kresten Nielsen, Kim F Haselmann
Data-independent mass spectral acquisition is particularly powerful when combined with ultra-performance liquid chromatography (LC) that provides excellent separation of most components present in a given sample. Data-independent analysis (DIA) consists of alternating full MS scans and scans with fragmentation of all ions within a selected m/z range, providing precursor masses and structure information, respectively. Fragmentation spectra are acquired either by sequential isolation and fragmentation of sliding m/z ranges or fragmenting all ions entering the MS instrument with no ion isolation, termed broadband DIA...
December 2, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27910792/optimization-of-liquid-fermentation-conditions-and-protein-nutrition-evaluation-of-mycelium-from-the-caterpillar-medicinal-mushroom-cordyceps-militaris-ascomycetes
#7
Jie Gang, Han Liu, Yanhong Liu
Cordyceps militaris is a well-known traditional Chinese medicinal mushroom. In this study, the mycelium of C. militaris was cultured using liquid fermentation technology and the culture medium components were optimized by the orthogonal test method. Our results showed that the optimal medium combination for the mycelium growth is 3% glucose, 3% peptone, 0.1% MgSO4, and 0.2% KH2PO4 The international general nutritional assessment method was applied to the overall evaluation of the protein nutrition value of submerged cultivated mycelium and fruit body of C...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27905109/a-lethal-neonatal-phenotype-of-mitochondrial-short-chain-enoyl-coa-hydratase-1-deficiency
#8
F Al Mutairi, H E Shamseldin, M Alfadhel, R J Rodenburg, F S Alkuraya
Short-chain enoyl-CoA hydratase (SCEH) is a mitochondrial enzyme involved in the oxidation of fatty acids and the catabolic pathway of valine and, to a lesser extent, isoleucine. Deficiency of this enzyme was recently shown to cause an early childhood Leigh syndrome phenotype. The few reported patients were compound heterozygotes for two missense or missense with truncating variants in ECHS1 that encodes SCEH. We describe two siblings with severe refractory lactic acidosis and death within the first 2 days of life...
October 13, 2016: Clinical Genetics
https://www.readbyqxmd.com/read/27898682/genetic-predisposition-to-an-impaired-metabolism-of-the-branched-chain-amino-acids-and-risk-of-type-2-diabetes-a-mendelian-randomisation-analysis
#9
Luca A Lotta, Robert A Scott, Stephen J Sharp, Stephen Burgess, Jian'an Luan, Therese Tillin, Amand F Schmidt, Fumiaki Imamura, Isobel D Stewart, John R B Perry, Luke Marney, Albert Koulman, Edward D Karoly, Nita G Forouhi, Rasmus J O Sjögren, Erik Näslund, Juleen R Zierath, Anna Krook, David B Savage, Julian L Griffin, Nishi Chaturvedi, Aroon D Hingorani, Kay-Tee Khaw, Inês Barroso, Mark I McCarthy, Stephen O'Rahilly, Nicholas J Wareham, Claudia Langenberg
BACKGROUND: Higher circulating levels of the branched-chain amino acids (BCAAs; i.e., isoleucine, leucine, and valine) are strongly associated with higher type 2 diabetes risk, but it is not known whether this association is causal. We undertook large-scale human genetic analyses to address this question. METHODS AND FINDINGS: Genome-wide studies of BCAA levels in 16,596 individuals revealed five genomic regions associated at genome-wide levels of significance (p < 5 × 10-8)...
November 2016: PLoS Medicine
https://www.readbyqxmd.com/read/27896282/a-patient-with-lissencephaly-developmental-delay-and-infantile-spasms-due-to-de-novo-heterozygous-mutation-of-kif2a
#10
Guoling Tian, Ana G Cristancho, Holly A Dubbs, Grant T Liu, Nicholas J Cowan, Ethan M Goldberg
BACKGROUND: Microtubules are dynamic polymers of α/β tubulin heterodimers that play a critical role in cerebral cortical development, by regulating neuronal migration, differentiation, and morphogenesis. Mutations in genes that encode either α- or β-tubulin or a spectrum of proteins involved in the regulation of microtubule dynamics lead to clinically devastating malformations of cortical development, including lissencephaly. METHODS: This is a single case report or a patient with lissencephaly, developmental delay, nystagmus, persistent hyperplastic primary vitreous, and infantile spasms, and undertook a neurogenetic workup...
November 2016: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/27891564/a-homozygous-pign-missense-mutation-in-soft-coated-wheaten-terriers-with-a-canine-paroxysmal-dyskinesia
#11
Ana L Kolicheski, Gary S Johnson, Tendai Mhlanga-Mutangadura, Jeremy F Taylor, Robert D Schnabel, Taroh Kinoshita, Yoshiko Murakami, Dennis P O'Brien
Hereditary paroxysmal dyskinesias (PxD) are a heterogeneous group of movement disorders classified by frequency, duration, and triggers of the episodes. A young-adult onset canine PxD has segregated as an autosomal recessive trait in Soft-Coated Wheaten Terriers. The medical records and videos of episodes from 25 affected dogs were reviewed. The episodes of hyperkinesia and dystonia lasted from several minutes to several hours and could occur as often as >10/day. They were not associated with strenuous exercise or fasting but were sometimes triggered by excitement...
November 28, 2016: Neurogenetics
https://www.readbyqxmd.com/read/27886326/molecular-dynamics-simulation-of-amino-acid-ionic-liquids-near-a-graphene-electrode-effects-of-alkyl-side-chain-length
#12
Behnoosh Sadeghi Moghadam, Mohammad Razmkhah, Mohammad Taghi Hamed Mosavian, Fatemeh Moosavi
Electric double layer (EDL) supercapacitors, using ionic liquid electrolytes, have been receiving a great deal of attention in response to the growing demand for energy storage systems. In the present study, the nanoscopic structure of amino acid ionic liquids (AAILs) as biodegradable electrolytes near a neutral graphene surface was studied by molecular dynamics (MD) simulation. In order to explore the influence of the anion type and structure, the effect of the alkyl side-chain length of amino acids on the EDL was investigated...
November 25, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27881392/higher-serum-phenylalanine-concentration-is-associated-with-more-rapid-telomere-shortening-in-men
#13
Johan G Eriksson, Maria-Angela Guzzardi, Patricia Iozzo, Eero Kajantie, Hannu Kautiainen, Minna K Salonen
BACKGROUND: Telomere length and telomere shortening are associated with age-related health outcomes. Only a few studies have been able to longitudinally report on factors that are associated with changes in telomere length in an aging population. OBJECTIVE: We studied the longitudinal relation between telomere length, the change in telomere length, and circulating amino acids. DESIGN: A total of 812 subjects from the Helsinki Birth Cohort Study (born from 1934 to 1944), who underwent 3 clinical visits during a 10-y interval that included measurements of cardiometabolic risk factors, were included in the study...
November 23, 2016: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27881338/-plasma-metabonomics-of-guifu-dihuang-wan-in-the-treatment-of-yang-deficiency
#14
Ya Xiao, Yuan Jing, Jie-Yu Chen, Fei Li, Jing-Ru Cheng, Jian-Lu Bi, Ren Luo, Xiao-Shan Zhao
OBJECTIVE: To assess the effect of Guifu Dihuang Wan (GFDHW) in the treatment of yang deficiency and explore the underlying molecular mechanism. METHODS: Sixty-two participants without diseases were randomized into control group (n=31) and experimental group (n=31) and were given lifestyle intervention additional GFDHW treatment for a month. NMR technology was used for metabonomics analysis. RESULTS: Intervention with GFDHW resulted in significantly decreased conversion scores of yang deficiency in the experimental group compared with the control group (P<0...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27876530/two-adjacent-phenylalanines-in-the-nmda-receptor-glun2a-subunit-m3-domain-interactively-regulate-alcohol-sensitivity-and-ion-channel-gating
#15
Hong Ren, Yulin Zhao, Man Wu, Donard S Dwyer, Robert W Peoples
The N-methyl-d-aspartate (NMDA) receptor is a key target of ethanol action in the central nervous system. Alcohol inhibition of NMDA receptor function involves small clusters of residues in the third and fourth membrane-associated (M) domains. Previous results from this laboratory have shown that two adjacent positions in the M3 domain, F636 and F637, can powerfully regulate alcohol sensitivity and ion channel gating. In this study, we report that these positions interact with one another in the regulation of both NMDA receptor gating and alcohol action...
November 19, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/27874020/friedelin-synthase-from-maytenus-ilicifolia-leucine-482-plays-an-essential-role-in-the-production-of-the-most-rearranged-pentacyclic-triterpene
#16
Tatiana M Souza-Moreira, Thaís B Alves, Karina A Pinheiro, Lidiane G Felippe, Gustavo M A De Lima, Tatiana F Watanabe, Cristina C Barbosa, Vânia A F F M Santos, Norberto P Lopes, Sandro R Valentini, Rafael V C Guido, Maysa Furlan, Cleslei F Zanelli
Among the biologically active triterpenes, friedelin has the most-rearranged structure produced by the oxidosqualene cyclases and is the only one containing a cetonic group. In this study, we cloned and functionally characterized friedelin synthase and one cycloartenol synthase from Maytenus ilicifolia (Celastraceae). The complete coding sequences of these 2 genes were cloned from leaf mRNA, and their functions were characterized by heterologous expression in yeast. The cycloartenol synthase sequence is very similar to other known OSCs of this type (approximately 80% identity), although the M...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27872960/branched-chain-amino-acids
#17
Keisuke Yamamoto, Atsunari Tsuchisaka, Hideaki Yukawa
Branched-chain amino acids (BCAAs), viz., L-isoleucine, L-leucine, and L-valine, are essential amino acids that cannot be synthesized in higher organisms and are important nutrition for humans as well as livestock. They are also valued as synthetic intermediates for pharmaceuticals. Therefore, the demand for BCAAs in the feed and pharmaceutical industries is increasing continuously. Traditional industrial fermentative production of BCAAs was performed using microorganisms isolated by random mutagenesis. A collection of these classical strains was also scientifically useful to clarify the details of the BCAA biosynthetic pathways, which are tightly regulated by feedback inhibition and transcriptional attenuation...
November 22, 2016: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/27870266/potential-urinary-biomarkers-of-nephrotoxicity-in-cyclophosphamide-treated-rats-investigated-by-nmr-based-metabolic-profiling
#18
Sa Rang Lim, Sun-Hee Hyun, Seul Gi Lee, Jin-Young Kim, So-Hyun Kim, Sang-Jin Park, Kyoung-Sik Moon, Donggeun Sul, Dong Hyun Kim, Hyung-Kyoon Choi
The anticancer-drug cyclophosphamide (CP) is known to have nephrotoxicity. The aim of this study was to identify urinary biomarkers indicating CP-induced nephrotoxicity. We investigated the urine metabolic profiles using nuclear magnetic resonance spectrometry of rats administered with single high-doses of CP (0, 30, and 100 mg/kg body weight) and daily low-doses over a 4-week period (0, 1, 3, and 10 mg/kg body weight). Among 18 identified urinary metabolites, 2-oxoglutarate, citrate, hippurate, formate, valine, and alanine for short-term and 2-oxoglutarate, citrate, hippurate, isoleucine, leucine, allantoin, valine, and lysine for long-term were selected as potential biomarkers...
November 21, 2016: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/27867938/a-mutation-in-npas3-that-segregates-with-schizophrenia-in-a-small-family-leads-to-protein-aggregation
#19
Leslie G Nucifora, YeeWen Candace Wu, Brian J Lee, Li Sha, Russell L Margolis, Christopher A Ross, Akira Sawa, Frederick C Nucifora
Schizophrenia and other major mental illnesses result from a complex interplay of genetic and environmental factors. We previously identified a mutation in NPAS3 that results in a valine to isoleucine (V304I) amino acid substitution segregating with schizophrenia in a small family. The amino acid change occurs in a potentially critical region for protein function. Furthermore, the same amino acid substitution in proteins related to familial Alzheimer's disease and transthyretin amyloidosis has been associated with protein aggregation...
October 2016: Molecular Neuropsychiatry
https://www.readbyqxmd.com/read/27865507/plasma-protein-and-supplemental-isoleucine-in-milk-replacers-for-dairy-calves
#20
K M Vasquez, S Y Morrison, J M Campbell, J K Drackley
We measured the effects of milk replacers containing 0, 33, 66, or 100% of the total replaceable whey protein as bovine plasma protein (PP), without or with Ile supplementation, on the intake, growth, and health of 124 male Holstein calves for 35 d. Milk replacers were formulated to contain 18% crude protein and 20% fat, with contents of Lys and Met equalized. When fed to calves at 1.5% of body weight (dry matter basis) under thermoneutral conditions, diets were predicted to allow average daily gains of 0.55 kg/d based on metabolizable energy or 0...
November 16, 2016: Journal of Dairy Science
keyword
keyword
48410
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"