keyword
MENU ▼
Read by QxMD icon Read
search

cucumber

keyword
https://www.readbyqxmd.com/read/28109844/colorimetric-detection-of-cucumber-green-mottle-mosaic-virus-using-unmodified-gold-nanoparticles-as-colorimetric-probes
#1
Lin Wang, Zhanmin Liu, Xueying Xia, Cuiyun Yang, Junyi Huang, Sibao Wan
Cucumber green mottle mosaic virus (CGMMV)causes a severe mosaic symptom of watermelon and cucumber, and can be transmitted via infected cucumber seeds, leaves and soil. It remains a challenge to detect this virus to prevent its introduction and infection and spread in fields. For this purpose, a simple and sensitive label-free colorimetric detection method for CGMMV has been developed with unmodified gold nanoparticles (AuNPs) as colorimetric probes. The method is based on the finding that the presence of RT-PCR target products of CGMMV and species-specific probes results in color change of AuNPs from red to blue after NaCl induction...
January 18, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28109843/development-of-a-molecular-assay-for-the-detection-of-cucumber-mosaic-virus-and-the-discrimination-of-its-subgroups-i-and-ii
#2
N Bald-Blume, J H W Bergervoet, E Maiss
A nucleic acid based test for the detection of the economically important plant virus Cucumber mosaic virus (CMV) based on the Luminex xTAG technology was developed. This technology has the advantage of allowing the simultaneous detection of various targets. Applying this method, we prove the presence of CMV in general and differentiate between its two subgroups I and II for which significant differences concerning severity of symptoms and virulence have been reported. For the development of the test procedure the coat protein gene sequences of 29 CMV isolates were cloned, sequenced and classified into subgroups...
January 18, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28109755/construction-of-agrobacterium-tumefaciens-mediated-tomato-black-ring-virus-infectious-cdna-clones
#3
Aleksandra Zarzyńska-Nowak, Inmaculada Ferriol, Bryce W Falk, Natasza Borodynko-Filas, Beata Hasiów-Jaroszewska
Tomato black ring virus (TBRV, genus Nepovirus) infects a wide range of economically important plants such as tomato, potato, tobacco and cucumber. Here, a successful construction of infectious full-length cDNA clones of the TBRV genomic RNAs (RNA1 and RNA2) is reported for the first time. The engineered constructs consisting of PCR-amplified DNAs were cloned into binary vector pJL89 immediately downstream of a double Cauliflower mosaic virus (CaMV) 35S promoter, and upstream of the Hepatitis delta virus (HDV) ribozyme and nopaline synthase terminator (NOS)...
January 18, 2017: Virus Research
https://www.readbyqxmd.com/read/28108247/design-synthesis-and-bioactivities-of-novel-oxadiazole-substituted-pyrazole-oximes
#4
Hong Dai, Jia Chen, Gang Li, Shushan Ge, Yujun Shi, Yuan Fang, Yong Ling
A series of novel pyrazole oxime derivatives containing a substituted oxadiazole group were designed and synthesized. The bioassay results indicated that some title compounds displayed good acaricidal and insecticidal activities against Tetranychus cinnabarinus, Aphis medicaginis, Oriental armyworm, and Nilaparvata lugens. Especially, compounds 7a, 7b, and 7c had 80%, 90%, and 90% insecticidal activities against A. medicaginis at 20μg/mL, respectively. Interestingly, some of the designed compounds displayed wonderful fungicidal activities in vivo against cucumber Pseudoperonospora cubensis...
December 31, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28107986/dispersive-solid-phase-extraction-using-polyaniline-modified-zeolite-nay-as-a-new-sorbent-for-multiresidue-analysis-of-pesticides-in-food-and-environmental-samples
#5
Prapha Arnnok, Nopbhasinthu Patdhanagul, Rodjana Burakham
The applicability of polyaniline (PANI)-modified zeolite NaY as a sorbent for multi-class pesticides extraction was investigated. The sorbent had a sorption capacity of 833mgkg(-1) sorbent and preconcentration factor of 42 in the mixture of twenty pesticides. The PANI-coated zeolite NaY was successfully created via oxidative polymerization of aniline onto the surface of the zeolite. The sorbent was applied for dispersive solid phase extraction (DSPE) of commonly used pesticides belonging to five different chemical groups, involving carbamate, organophosphate, sulfonylurea, pyrethroid and neonicotinoid...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107966/novel-potentiometric-sensors-for-the-determination-of-the-dinotefuran-insecticide-residue-levels-in-cucumber-and-soil-samples
#6
Maha F Abdel-Ghany, Lobna A Hussein, Noha F El Azab
Five new potentiometric membrane sensors for the determination of the dinotefuran levels in cucumber and soil samples have been developed. Four of these sensors were based on a newly designed molecularly imprinted polymer (MIP) material consisting of acrylamide or methacrylic acid as a functional monomer in a plasticized PVC (polyvinyl chloride) membrane before and after elution of the template. A fifth sensor, a carboxylated PVC-based sensor plasticized with dioctyl phthalate, was also prepared and tested...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107942/stereoselective-quantification-of-triticonazole-in-vegetables-by-supercritical-fluid-chromatography
#7
Qi Tan, Jun Fan, Ruiqi Gao, Rujian He, Tai Wang, Yaomou Zhang, Weiguang Zhang
A highly fast analytical method though supercritical fluid chromatography (SFC) has been developed to quantify triticonazole enantiomers in cucumbers and tomatoes. Effects of organic modifier type and concentration on chiral separation and quantification of standard solution as well as matrix-matched standard solutions have been studied in detail. Among three organic modifiers, better separation of triticonazole racemate was achieved with 20% ethanol (v/v). The run time in SFC (ca 3min) with CO2-ethanol (80:20, v/v) as the mobile phase was six-fold shorter than HPLC analysis (about 18min)...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28105959/expression-of-an-extracellular-ribonuclease-gene-increases-resistance-to-cucumber-mosaic-virus-in-tobacco
#8
Teppei Sugawara, Ekaterina A Trifonova, Alex V Kochetov, Yoshinori Kanayama
BACKGROUND: The apoplast plays an important role in plant defense against pathogens. Some extracellular PR-4 proteins possess ribonuclease activity and may directly inhibit the growth of pathogenic fungi. It is likely that extracellular RNases can also protect plants against some viruses with RNA genomes. However, many plant RNases are multifunctional and the direct link between their ribonucleolytic activity and antiviral defense still needs to be clarified. In this study, we evaluated the resistance of Nicotiana tabacum plants expressing a non-plant single-strand-specific extracellular RNase against Cucumber mosaic virus...
November 16, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/28102426/improved-anticoagulant-effect-of-fucosylated-chondroitin-sulfate-orally-administered-as-gastro-resistant-tablets
#9
Roberto J C Fonseca, Isabela D Sucupira, Stephan Nicollas M C G Oliveira, Gustavo R Santos, Paulo A S Mourão
Fucosylated chondroitin sulfate (FucCS) is a potent anticoagulant polysaccharide extracted from sea cucumber. Its anticoagulant activity is attributed to the presence of unique branches of sulfated fucose. Although this glycosaminoglycan exerts an antithrombotic effect following oral administration, high doses are necessary to achieve the maximum effect. The diminished activity of FucCS following oral administration is likely due to its degradation in the gastrointestinal tract and its limited ability to cross the intestinal cell membranes...
January 19, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28100310/growth-inhibition-of-fungus-phycomyces-blakesleeanus-by-anion-channel-inhibitors-anthracene-9-carboxylic-and-niflumic-acid-attained-through-decrease-in-cellular-respiration-and-energy-metabolites
#10
Marina Stanić, Strahinja Križak, Mirna Jovanović, Tanja Pajić, Ana Ćirić, Milan Žižić, Joanna Zakrzewska, Tijana Cvetić Antić, Nataša Todorović, Miroslav Živić
Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 or 500 µM NFA, for growth and respiration, respectively...
January 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28089747/metabolic-engineering-of-a-diazotrophic-bacterium-improves-ammonium-release-and-biofertilization-of-plants-and-microalgae
#11
Rafael Ambrosio, Juan Cesar Federico Ortiz-Marquez, Leonardo Curatti
The biological nitrogen fixation carried out by some Bacteria and Archaea is one of the most attractive alternatives to synthetic nitrogen fertilizers. However, with the exception of the symbiotic rhizobia-legumes system, progress towards a more extensive realization of this goal has been slow. In this study we manipulated the endogenous regulation of both nitrogen fixation and assimilation in the aerobic bacterium Azotobacter vinelandii. Substituting an exogenously inducible promoter for the native promoter of glutamine synthetase produced conditional lethal mutant strains unable to grow diazotrophically in the absence of the inducer...
January 9, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28076417/colonization-of-wheat-maize-and-cucumber-by-paenibacillus-polymyxa-wly78
#12
Tianyi Hao, Sanfeng Chen
Paenibacillus polymyxa WLY78 is a nitrogen fixer and it can be potentially applied to biofertilizer in agriculture. In this study, P. polymyxa WLY78 is labelled with gfp gene. The GFP-labelled P. polymyxa WLY78 is used to inoculate wheat, maize and cucumber seedlings grown in the gnotobiotic system and in soil, respectively. Observation by confocal laser scanning microscope reveals that the GFP-labeled bacterial cells are mainly located on the root surface and epidermis of wheat, and only a few cells are present within cortical cells...
2017: PloS One
https://www.readbyqxmd.com/read/28074264/nacl-stress-induces-cssams-gene-expression-in-cucumis-sativus-by-mediating-the-binding-of-csgt-3b-to-the-gt-1-element-within-the-cssams-promoter
#13
Li-Wei Wang, Mei-Wen He, Shi-Rong Guo, Min Zhong, Sheng Shu, Jin Sun
The CsSAMs promoter is a salt-stress-inducible promoter containing three GT-1 elements that are sufficient for the salt-stress response. The transcription factor CsGT-3b was found to bind to the GT-1 element. The S-adenosyl-L-methionine synthase (SAMs) gene is among the functional genes induced during environmental stress. However, little is known about the regulatory mechanism and upstream regulators of this salt-inducible gene in cucumber plants. Thus, it is necessary to understand the characteristics of the SAMs gene by analyzing its promoter and transcription factors...
January 10, 2017: Planta
https://www.readbyqxmd.com/read/28071648/foliar-application-of-the-leaf-colonizing-yeast-pseudozyma-churashimaensis-elicits-systemic-defense-of-pepper-against-bacterial-and-viral-pathogens
#14
Gahyung Lee, Sang-Heon Lee, Kyung Mo Kim, Choong-Min Ryu
Yeast associates with many plant parts including the phyllosphere, where it is subject to harsh environmental conditions. Few studies have reported on biological control of foliar pathogens by yeast. Here, we newly isolated leaf-colonizing yeasts from leaves of field-grown pepper plants in a major pepper production area of South Korea. The yeast was isolated using semi-selective medium supplemented with rifampicin to inhibit bacterial growth and its disease control capacity against Xanthomonas axonopodis infection of pepper plants in the greenhouse was evaluated...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28059140/identification-and-functional-characterisation-of-5-ht4-receptor-in-sea-cucumber-apostichopus-japonicus-selenka
#15
Tianming Wang, Zhen Yang, Naiming Zhou, Lina Sun, Zhenming Lv, Changwen Wu
Serotonin (5-HT) is an important neurotransmitter and neuromodulator that controls a variety of sensory and motor functions through 5-HT receptors (5-HTRs). The 5-HT4R subfamily is linked to Gs proteins, which activate adenylyl cyclases (ACs), and is involved in many responses in peripheral organs. In this study, the 5-HT4R from Apostichopus japonicus (Aj5-HT4R) was identified and characterised. The cloned full-length Aj5-HT4R cDNA is 1,544 bp long and contains an open reading frame 1,011 bp in length encoding 336 amino acid proteins...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28059067/viral-effector-protein-manipulates-host-hormone-signaling-to-attract-insect-vectors
#16
Dewei Wu, Tiancong Qi, Wan-Xiang Li, Haixia Tian, Hua Gao, Jiaojiao Wang, Jin Ge, Ruifeng Yao, Chunmei Ren, Xian-Bing Wang, Yule Liu, Le Kang, Shou-Wei Ding, Daoxin Xie
Some plant and animal pathogens can manipulate their hosts to cause them to release odors that are attractive to the pathogens' arthropod vectors. However, the molecular mechanism underlying this process is largely unexplored, and the specific effectors the pathogens employ as well as the pathways within the hosts they target are currently unknown. Here we reveal that the aphid-borne cucumber mosaic virus (CMV) employs its 2b protein, a well-characterized viral suppressor of host RNA interference (VSR), to target the host's jasmonate (JA) hormone pathway, thus acting as a viral inducer of host attractiveness to insect vectors (VIA)...
January 6, 2017: Cell Research
https://www.readbyqxmd.com/read/28056792/elucidation-of-the-molecular-responses-of-a-cucumber-segment-substitution-line-carrying-pm5-1-and-its-recurrent-parent-triggered-by-powdery-mildew-by-comparative-transcriptome-profiling
#17
Qiang Xu, Xuewen Xu, Yang Shi, Xiaohua Qi, Xuehao Chen
BACKGROUND: Powdery mildew (PM) is one of the most severe fungal diseases of cucurbits, but the molecular mechanisms underlying PM resistance in cucumber remain elusive. In this study, we developed a PM resistant segment substitution line SSL508-28 that carried a segment on chromosome five representing the Pm5.1 locus from PM resistant donor Jin5-508 using marker-assisted backcrossing of an elite PM susceptible cucumber inbred line D8. RESULTS: Whole-genome resequencing of SSL508-28, Jin5-508 and D8 was performed to identify the exact boundaries of the breakpoints for this introgression because of the low density of available single sequence repeat markers...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28055187/synthesis-of-1-2-3-thiadizole-and-thiazole-based-strobilurins-as-potent-fungicide-candidates
#18
Lai Chen, Yu-Jie Zhu, Zhijin Fan, Xiao-Feng Guo, Zhi-Ming Zhang, Jing-Hua Xu, Ying-Qi Song, Yury Yurievich Morzherin, Nataliya P Belskaya, Vasiliy A Bakulev
Strobilurin fungicides play a crucial role in protecting plants against different pathogens and securing food supplies. A series of 1,2,3-thiadiazole and thiazole based strobilurins were rationally designed, synthesized, characterized and tested against various fungi. Introduction of 1,2,3-thiadiazole greatly improved the fungicidal activity of the target molecules. Compounds 8a, 8c, 8d and 10i exhibited a relative broad-spectrum of fungicidal activity. Compound 8a showed excellent activities against Gibberella zeae, Sclerotinia sclerotiorum and Rhizoctonia cerealis with the median effective concentration (EC50) of 2...
January 5, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28054163/genetic-variation-and-population-structure-of-cucumber-green-mottle-mosaic-virus
#19
Li-Xia Rao, Yushuang Guo, Li-Li Zhang, Xue-Ping Zhou, Jian Hong, Jian-Xiang Wu
Cucumber green mottle mosaic virus (CGMMV) is a single-stranded, positive sense RNA virus infecting cucurbitaceous plants. In recent years, CGMMV has become an important pathogen of cucurbitaceous crops including watermelon, pumpkin, cucumber and bottle gourd in China, causing serious losses to their production. In this study, we surveyed CGMMV infection in various cucurbitaceous crops grown in Zhejiang Province and in several seed lots purchased from local stores with the dot enzyme-linked immunosorbent assay (dot-ELISA), using a CGMMV specific monoclonal antibody...
January 4, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28053573/antibiotic-resistance-profiling-and-phenotyping-of-aeromonas-species-isolated-from-aquatic-sources
#20
Olumide A Odeyemi, Asmat Ahmad
This study aimed to investigate antibiotics resistance pattern and phenotyping of Aeromonas species isolated from different aquatic sources in Melaka, Malaysia. A total of 53 Aeromonas species were isolated from the following sources: sediment (n = 13), bivalve (n = 10), sea cucumber (n = 16) and sea water (n = 14) and resistance to 12 antibiotics - Tetracycline (30 μg), Kanamycin (30 μg), Oxytetracycline (30 μg), Ampicillin (10 μg), Streptomycin (10 μg), Gentamicin (10 μg), Sulphamethoxazole (25 μg), Nalixidic acid (30 μg), Trimethoprim (1...
January 2017: Saudi Journal of Biological Sciences
keyword
keyword
48405
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"