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https://www.readbyqxmd.com/read/29053370/cross-contamination-and-biofilm-formation-by-salmonella-enterica-serovar-enteritidis-on-various-cutting-boards
#1
Stéfani T A Dantas, Bruna F Rossi, Erika C R Bonsaglia, Ivana G Castilho, Rodrigo T Hernandes, Ary Fernandes Júnior, Vera L M Rall
Cross-contamination is one of the main factors related to foodborne outbreaks. This study aimed to analyze the cross-contamination process of Salmonella enterica serovar Enteritidis from poultry to cucumbers, on various cutting board surfaces (plastic, wood, and glass) before and after washing and in the presence and absence of biofilm. Thus, 10 strains of Salmonella Enteritidis were used to test cross-contamination from poultry to the cutting boards and from thereon to cucumbers. Moreover, these strains were evaluated as to their capacity to form biofilm on hydrophobic (wood and plastic) and hydrophilic materials (glass)...
October 20, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29052099/the-novel-ethylene-responsive-factor-cserf025-affects-the-development-of-fruit-bending-in-cucumber
#2
Chunhua Wang, Ming Xin, Xiuyan Zhou, Chunhong Liu, Shengnan Li, Dong Liu, Yuan Xu, Zhiwei Qin
Overexpression of CsERF025 induces fruit bending by promoting the production of ethylene. Cucumber fruit bending critically affects cucumber quality, but the mechanism that causes fruit bending remains unclear. To better understand this mechanism, we performed transcriptome analyses on tissues from the convex (C1) and concave (C2) sides of bending and straight (S) fruit at 2 days post anthesis (DPA). We identified a total of 281 differentially expressed genes (DEGs) from both the convex and concave sides of bent fruit that showed significantly different expression profiles relative to straight fruits...
October 19, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/29050584/molecular-size-is-important-for-the-safety-and-selective-inhibition-of-intrinsic-factor-xase-for-fucosylated-chondroitin-sulfate
#3
Lufeng Yan, Junhui Li, Danli Wang, Tian Ding, Yaqin Hu, Xingqian Ye, Robert J Linhardt, Shiguo Chen
Fucosylated chondroitin sulfate from sea cucumber Isostichopus badionotus (FCS-Ib) showed potent anticoagulant activities without selectivity. The present study focused on developing safe FCS-Ib oligomers showing selective inhibition of intrinsic factor Xase (anti-FXase) prepared through partial N-deacetylation-deaminative cleavage. The N-deacetylation degree was regulated by reaction time, controlling the resulting oligomer distribution. Structure analysis confirmed the selectivity of degradation, and 12 high purity fractions with trisaccharide-repeating units were separated...
December 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29044170/phosphorylation-regulates-the-subcellular-localization-of-cucumber-mosaic-virus-2b-protein
#4
Katalin Nemes, Ákos Gellért, Asztéria Almási, Pál Vági, Réka Sáray, Katalin Kádár, Katalin Salánki
The 2b protein of Cucumber mosaic virus has a role in nearly all steps of the viral cycle including cell-to-cell movement, symptom induction and suppression of antiviral RNA silencing. Previous studies demonstrated the presence of 2b protein in the nucleus and in cytoplasm as well. Phosphorylation site of 2b protein is conserved in all CMV isolates, including proposed constitute motifs for casein kinase II and cyclin-dependent kinase 2. To discern the impact of 2b protein phosphorylation, we created eight different mutants to mimic the non-phosporylated (serine to alanine) as well as the phosphorylated state (serine to aspartic acid) of the protein...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29039760/sea-cucumber-glycosides-chemical-structures-producing-species-and-important-biological-properties
#5
REVIEW
Muhammad Abdul Mojid Mondol, Hee Jae Shin, M Aminur Rahman, Mohamad Tofazzal Islam
Sea cucumbers belonging to echinoderm are traditionally used as tonic food in China and other Asian countries. They produce abundant biologically active triterpene glycosides. More than 300 triterpene glycosides have been isolated and characterized from various species of sea cucumbers, which are classified as holostane and nonholostane depending on the presence or absence of a specific structural unit γ(18,20)-lactone in the aglycone. Triterpene glycosides contain a carbohydrate chain up to six monosaccharide units mainly consisting of d-xylose, 3-O-methy-d-xylose, d-glucose, 3-O-methyl-d-glucose, and d-quinovose...
October 17, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29037503/growth-kinetics-of-escherichia-coli-o157-h7-on-the-epicarp-of-fresh-vegetables-and-fruits
#6
Mariel Gullian-Klanian, Maria José Sánchez-Solis
Despite the increasing reports on the incidence of fresh vegetables and fruits as a possible vehicle for human pathogens, there is currently limited knowledge on the growth potential of Escherichia coli O157:H7 on different plant substrates. This study analyzed the selective adhesion and growth of E. coli O157:H7 on chili habanero (Capsicum chinense L.), cucumber (Cucumis sativus), radish (Raphanus sativus), tomato (Lycopersicon esculentum), beet (Beta vulgaris subsp. vulgaris), and onion (Allium cepa L.) under laboratory conditions...
August 30, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29035293/identification-of-z-2-3-diphenylacrylonitrileas-anti-cancer-molecule-in-persian-gulf-sea-cucumber-holothuria-parva
#7
Salimeh Amidi, Zahra Hashemi, Abbasali Motallebi, Melika Nazemi, Hoda Farrokhpayam, Enayatollah Seydi, Jalal Pourahmad
Hepatocellular carcinoma (HCC), also named cancerous hepatoma, is the most common type of malignant neoplasia of the liver. In this research, we screened the Persian Gulf sea cucumber Holothuria parva (H. parva) methanolic sub-fractions for the possible existence of selective toxicity on liver mitochondria isolated from an animal model of HCC. Next, we purified the most active fraction. Thus the structure of the active molecule was identified. HCC was induced by diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF) protocol...
October 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29033662/a-valid-name-for-the-xishuangbanna-gourd-a-cucumber-with-carotene-rich-fruits
#8
Susanne S Renner
Herbarium specimens deposited in publicly accessible collections are the basis for all scientific names because only permanent specimens can be re-studied by independent researchers, the very essence of science. Re-investigations may be done with morphological, chemical, genomic, computer-tomographic, or other methods. Based on new herbarium material, I here provide a name for the Xishuangbanna gourd, a plant long cultivated in Yunnan because of its large non-bitter fruits, rich in β-carotene. Genome re-sequencing of numerous accessions has shown that this cucumber mutant is closer to Cucumis sativus var...
2017: PhytoKeys
https://www.readbyqxmd.com/read/29030041/the-application-of-chiral-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-to-the-separation-of-the-zoxamide-enantiomers-and-the-study-of-enantioselective-degradation-process-in-agricultural-plants
#9
Xinglu Pan, Fengshou Dong, Zenglong Chen, Jun Xu, Xingang Liu, Xiaohu Wu, Yongquan Zheng
In this study, an effective and sensitive chiral analytical method was developed to detect zoxamide enantiomers in vegetables, fruits and environmental matrices using ultra-high-performance liquid chromatography-tandem mass spectrometry. Optimal separation conditions were achieved with Lux Amylose-2 chiral column using acetonitrile/water (70:30v/v) as mobile phase with a flow rate and column temperature of 0.5mL/min and 25°C. The absolute configuration, optical rotation and elution order were confirmed for the first time...
October 6, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29029269/an-overlooked-paleo-tetraploidization-in-cucurbitaceae
#10
Jinpeng Wang, Pengchuan Sun, Yuxian Li, Yinzhe Liu, Nanshan Yang, Jigao Yu, Xuelian Ma, Sangrong Sun, Ruiyan Xia, Xiaojian Liu, Dongcen Ge, Sainan Luo, Yinmeng Liu, Youting Kong, Xiaobo Cui, Tianyu Lei, Li Wang, Zhenyi Wang, Weina Ge, Lan Zhang, Xiaoming Song, Min Yuan, Di Guo, Dianchuan Jin, Wei Chen, Yuxin Pan, Tao Liu, Guixian Yang, Yue Xiao, Jinshuai Sun, Cong Zhang, Zhibo Li, Haiqing Xu, Xueqian Duan, Shaoqi Shen, Zhonghua Zhang, Sanwen Huang, Xiyin Wang
Cucurbitaceae plants are of considerable biological and economic importance, and genomes of cucumber, watermelon, and melon have been sequenced. However, a comparative genomics exploration of their genome structures and evolution has not been available. Here, we aimed at performing a hierarchical inference of genomic homology resulted from recursive paleo-polyploidizations. Unexpectedly, we found that, shortly after a core-eudicot-common hexaploidy (ECH), a cucurbit-common tetraploidization (CCT) occurred, overlooked by previous reports...
September 28, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29028871/high-light-aggravates-functional-limitations-of-cucumber-canopy-photosynthesis-under-salinity
#11
Tsu-Wei Chen, Hartmut Stützel, Katrin Kahlen
Background and Aims: Most crop species are glycophytes, and salinity stress is one of the most severe abiotic stresses reducing crop yields worldwide. Salinity affects plant architecture and physiological functions by different mechanisms, which vary largely between crop species and determine the susceptibility or tolerance of a crop species to salinity. Methods: Experimental data from greenhouse cucumber (Cucumis sativus), a salt-sensitive species, grown under three salinity levels were interpreted by combining a functional-structural plant model and quantitative limitation analysis of photosynthesis...
September 19, 2017: Annals of Botany
https://www.readbyqxmd.com/read/29027740/effective-tolerance-based-on-resource-reallocation-is-a-virus-specific-defence-in-arabidopsis-thaliana
#12
Aayushi Shukla, Israel Pagán, Fernando García-Arenal
Plant viruses often harm their hosts, which have developed mechanisms to prevent or minimise the effects of virus infection. Resistance and tolerance are the two main plant defences to pathogens. Although resistance to plants viruses has been extensively studied, tolerance has received much less attention. Theory predicts that tolerance to low virulent parasites would be achieved through resource reallocation from growth to reproduction, whereas tolerance to high virulent parasites would be attained through shortening of the pre-reproductive period...
October 13, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/29024273/the-first-brain-species-comparisons-and-evolutionary-implications-for-the-enteric-and-central-nervous-systems
#13
REVIEW
J B Furness, M J Stebbing
BACKGROUND: The enteric nervous system (ENS) and the central nervous system (CNS) of mammals both contain integrative neural circuitry and similarities between them have led to the ENS being described as the brain in the gut. PURPOSE: To explore relationships between the ENS and CNS across the animal kingdom. We found that an ENS occurs in all animals investigated, including hydra, echinoderms and hemichordates that do not have a CNS. The general form of the ENS, which consists of plexuses of neurons intrinsic to the gut wall and an innervation that controls muscle movements, is similar in species as varied and as far apart as hydra, sea cucumbers, annelid worms, octopus and humans...
October 11, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29023486/the-sea-cucumber-genome-provides-insights-into-morphological-evolution-and-visceral-regeneration
#14
Xiaojun Zhang, Lina Sun, Jianbo Yuan, Yamin Sun, Yi Gao, Libin Zhang, Shihao Li, Hui Dai, Jean-François Hamel, Chengzhang Liu, Yang Yu, Shilin Liu, Wenchao Lin, Kaimin Guo, Songjun Jin, Peng Xu, Kenneth B Storey, Pin Huan, Tao Zhang, Yi Zhou, Jiquan Zhang, Chenggang Lin, Xiaoni Li, Lili Xing, Da Huo, Mingzhe Sun, Lei Wang, Annie Mercier, Fuhua Li, Hongsheng Yang, Jianhai Xiang
Apart from sharing common ancestry with chordates, sea cucumbers exhibit a unique morphology and exceptional regenerative capacity. Here we present the complete genome sequence of an economically important sea cucumber, A. japonicus, generated using Illumina and PacBio platforms, to achieve an assembly of approximately 805 Mb (contig N50 of 190 Kb and scaffold N50 of 486 Kb), with 30,350 protein-coding genes and high continuity. We used this resource to explore key genetic mechanisms behind the unique biological characters of sea cucumbers...
October 2017: PLoS Biology
https://www.readbyqxmd.com/read/29021804/calcium-and-calmodulin-are-involved-in-nitric-oxide-induced-adventitious-rooting-of-cucumber-under-simulated-osmotic-stress
#15
Lijuan Niu, Jian Yu, Weibiao Liao, Jihua Yu, Meiling Zhang, Mohammed M Dawuda
Osmotic stress is a major form of abiotic stress that adversely affects growth and development of plants and subsequently reduces yield and quality of crops. In this study, the effect of nitric oxide (NO) and calcium (Ca(2+)) on the process of adventitious rooting in cucumber (Cucumis sativus L.) under simulated osmotic stress was investigated. The results revealed that the effect of exogenous NO and Ca(2+) in promoting the development of adventitious roots in cucumber seedlings under simulated osmotic stress was dose-dependent, with a maximal biological response at 10 μM NO donor nitroprusside (SNP) or 200 μM Ca(2+)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29020752/erratum-to-draft-genome-of-the-sea-cucumber-apostichopus-japonicus-and-genetic-polymorphism-among-color-variants
#16
Jihoon Jo, Jooseong Oh, Hyun-Gwan Lee, Hyun-Hee Hong, Sung-Gwon Lee, Seongmin Cheon, Elizabeth M A Kern, Soyeong Jin, Sung-Jin Cho, Joong-Ki Park, Chungoo Park
No abstract text is available yet for this article.
October 1, 2017: GigaScience
https://www.readbyqxmd.com/read/29018930/a-highly-selective-electrochemical-sensor-based-on-molecularly-imprinted-polypyrrole-modified-gold-electrode-for-the-determination-of-glyphosate-in-cucumber-and-tap-water
#17
Chao Zhang, Yongxin She, Tengfei Li, Fengnian Zhao, Maojun Jin, Yirong Guo, Lufei Zheng, Shanshan Wang, Fen Jin, Hua Shao, Haijin Liu, Jing Wang
An electrochemical sensor based on molecularly imprinted polypyrrole (MIPPy) was developed for selective and sensitive detection of the herbicide glyphosate (Gly) in cucumber and tap water samples. The sensor was prepared via synthesis of molecularly imprinted polymers on a gold electrode in the presence of Gly as the template molecule and pyrrole as the functional monomer by cyclic voltammetry (CV). The sensor preparation conditions including the ratio of template to functional monomers, number of CV cycles in the electropolymerization process, the method of template removal, incubation time, and pH were optimized...
October 10, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28991209/design-synthesis-and-antifungal-activity-of-psoralen-derivatives
#18
Xiang Yu, Ya Wen, Chao-Gen Liang, Jia Liu, Yu-Bin Ding, Wei-Hua Zhang
A series of linear furanocoumarins with different substituents have been designed and synthesized. Their structures were confirmed by ¹H-NMR spectroscopy, high resolution mass spectra (EI-MS), IR, and X-ray single-crystal diffraction. All of the target compounds were evaluated in vitro for their antifungal activity against Rhizoctorzia solani, Botrytis cinerea, Alternaria solani, Gibberella zeae, Cucumber anthrax, and Alternaria leaf spot at 100 μg/mL, and some of the designed compounds exhibited potential antifungal activities...
October 9, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28991187/peptides-from-colochirus-robustus-enhance-immune-function-via-activating-cd3%C3%AE-and-zap-70-mediated-signaling-in-c57bl-6-mice
#19
Xiaogang Du, Fangliang Lian, Yunkun Li, Dong Li, Dayong Wu, Qunli Feng, Zhijiang Feng, Yun Li, Guixian Bu, Fengyan Meng, Xiaohan Cao, Zhiyu Chen, Xianyin Zeng
Colochirus robustus, a species of sea cucumber, has long been used in East and Southeast Asia as nutritious food as well as for certain medicinal purpose. Studies have shown a number of biological functions associated with consumption of sea cucumber, many of which are attributed to its major component, sea cucumber peptides (SCP). However, how SCP impacts immune system, which is critical for host defense, has not been defined. To address this issue, in the present study, we conducted comprehensive analysis of immune function after oral administration of SCP (0, 25, 50, and 75 mg/kg body weigh) for eight weeks in C57BL/6 mice...
October 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28988154/checking-the-detail-in-retail-occurrence-of-cryptosporidium-and-giardia-on-vegetables-sold-across-different-counters-in-chandigarh-india
#20
Kjersti Selstad Utaaker, Anil Kumar, Himanshu Joshi, Suman Chaudhary, Lucy J Robertson
Fresh produce has been recognized as a vehicle of infection for protozoan parasites, particularly Cryptosporidium, and, to a lesser extent, Giardia. For both parasites, outbreaks associated with fresh produce have been documented. Although documented outbreaks tend to be from industrialized countries, contamination of fresh produce with these parasites is a global issue. In developing countries, infections with these parasites are often endemic in the community, and basic infrastructure and hygiene measures may be inadequate, thus the likelihood of contamination of fresh produce with these parasites may be higher...
October 1, 2017: International Journal of Food Microbiology
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