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https://www.readbyqxmd.com/read/28633949/habitat-suitability-index-model-of-the-sea-cucumber-apostichopus-japonicus-selenka-a-case-study-of-shandong-peninsula-china
#1
Zhipeng Zhang, Jian Zhou, Jingjing Song, Qixiang Wang, Hongjun Liu, Xuexi Tang
A habitat suitability index (HSI) model for the sea cucumber Apostichopus japonicus (Selenka) was established in the present study. Based on geographic information systems, the HSI model was used to identify potential sites around the Shandong Peninsula suitable for restoration of immature (<25g) and mature (>25g) A. japonicus. Six habitat factors were used as input variables for the HSI model: sediment classification, water temperature, salinity, water depth, pH and dissolved oxygen. The weighting of each habitat factor was defined through the Delphi method...
June 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28623561/variation-in-cucumber-cucumis-sativus-l-fruit-size-and-shape-results-from-multiple-components-acting-pre-anthesis-and-post-pollination
#2
Marivi Colle, Yiqun Weng, Yunyan Kang, Ron Ophir, Amir Sherman, Rebecca Grumet
Morphological, QTL, and gene expression analyses indicate variation in cucumber fruit size and shape results from orientation, timing, and extent of cell division and expansion, and suggest candidate gene factors. Variation in cucumber (Cucumis sativus L.) fruit size and shape is highly quantitative, implicating interplay of multiple components. Recent studies have identified numerous fruit size and shape quantitative trait loci (QTL); however, underlying factors remain to be determined. We examined ovary and fruit development of two sequenced cucumber genotypes with extreme differences in fruit size and shape, Chinese Long '9930' (CL9930), and pickling type 'Gy14'...
June 16, 2017: Planta
https://www.readbyqxmd.com/read/28621835/exogenously-applied-dsrna-molecules-deriving-from-zucchini-yellow-mosaic-virus-genome-move-systemically-and-protect-cucurbits-against-zymv
#3
Athanasios Kaldis, Margarita Berbati, Ourania Melita, Chrysavgi Reppa, Maria Holeva, Patricia Otten, Andreas Voloudakis
Zucchini yellow mosaic virus (ZYMV) causes serious damage in a great number of cucurbits and control measures are necessary. Transgenic cucurbits expressing parts of ZYMV genome were shown to be resistant against the cognate virus. A non-transgenic approach involving exogenous application of dsRNA has also been shown to induce resistance in tobacco against cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV). In the present study, dsRNA molecules derived from the helper component-proteinase (HC-Pro) and coat protein (CP) genes of the ZYMV_DE_2014 isolate were produced in vitro...
June 16, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28615634/transcriptome-profiling-of-cucumis-metuliferus-infected-by-meloidogyne-incognita-provides-new-insights-into-putative-defense-regulatory-network-in-cucurbitaceae
#4
Jian Ling, Zhenchuan Mao, Mingjuan Zhai, Feng Zeng, Yuhong Yang, Bingyan Xie
Root-knot nematodes (RKN) represent extensive challenges to Cucurbitaceae crops. However, Cucumis metuliferus (Cm) is known to be resistant to Meloidogyne incognita (Mi) infections. Thus, analysis of differentially expressed genes may lead to a comprehensive gene expression profiling of the incompatible Cm-Mi interaction. In this study, the time-course transcriptome of Cm against Mi infection was monitored using RNA-Seq. More than 170000 transcripts were examined in Cm roots, and 2430 genes were subsequently identified as differentially expressed in response to Mi infection...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28608281/suppression-of-cucumber-stachyose-synthase-gene-cssts-inhibits-phloem-loading-and-reduces-low-temperature-stress-tolerance
#5
Jianguo Lü, Xiaolei Sui, Si Ma, Xin Li, Huan Liu, Zhenxian Zhang
Stachyose is the main transporting sugar in phloem of Raffinose family oligosaccharides-transporting species. Stachyose synthase (STS) is a key enzyme for stachyose biosynthesis, but the gene encoding STS is poorly characterized in cucumber (Cucumis sativus L.), which is a model plant for studying stachyose metabolism and phloem function. In this research, stachyose synthase gene (CsSTS) from cucumber was isolated and its physiological functions were analyzed. CsSTS expressed mainly in the phloem of the minor veins in mature leaves and localized to companion cells...
June 12, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28602183/analysis-of-trace-microcystins-in-vegetables-using-matrix-solid-phase-dispersion-followed-by-high-performance-liquid-chromatography-triple-quadrupole-mass-spectrometry-detection
#6
Zong-Yao Qian, Zhi-Gang Li, Jian Ma, Ting-Ting Gong, Qi-Ming Xian
A selective and sensitive method for the simultaneous determination of five common microcystins (MC-LR, -RR, -YR, -LW, -LF) in various vegetables was established using Matrix Solid-Phase Dispersion (MSPD) followed by high performance liquid chromatography-mass spectrometry detection. To optimize the pretreatment procedure for extracting the microcystins from three vegetable matrices (tomato, cucumber and spinacia, which represented different colors), Box-Behnken design was employed to optimize the main factors affecting the extraction efficiency, including sample/dispersant mass ratio, as well as the proportion mixture dispersant and the volumes of solvent...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28601631/ibt-based-quantitative-proteomics-identifies-potential-regulatory-proteins-involved-in-pigmentation-of-purple-sea-cucumber-apostichopus-japonicus
#7
Lili Xing, Lina Sun, Shilin Liu, Xiaoni Li, Libin Zhang, Hongsheng Yang
Sea cucumbers are an important economic species and exhibit high yield value among aquaculture animals. Purple sea cucumbers are very rare and beautiful and have stable hereditary patterns. In this study, isobaric tags (IBT) were first used to reveal the molecular mechanism of pigmentation in the body wall of the purple sea cucumber. We analyzed the proteomes of purple sea cucumber in early pigmentation stage (Pa), mid pigmentation stage (Pb) and late pigmentation stage (Pc), resulting in the identification of 5580 proteins, including 1099 differentially expressed proteins in Pb: Pa and 339 differentially expressed proteins in Pc: Pb...
June 2, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28598301/aquimarina-rubra-sp-nov-isolated-from-sediment-of-a-sea-cucumber-culture-pond
#8
Ji-Ru Han, Dong-Bo Fang, Hai-Feng Xia, Guan-Jun Chen, Zong-Jun Du
A Gram-stain-negative, non-motile, rod-shaped, red-pigmented, facultatively anaerobic bacterium, designated SS2-9T, was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, China. Cells of strain SS2-9T were approximately 0.3-0.5 µm in width and 1.5-6.0 µm in length. The strain was able to grow at 10-37 °C, at pH 6.5-8.5 and in the presence of 0.5-6.0 % (w/v) NaCl. It grew optimally at 28 °C and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content was 34...
June 9, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28594569/presence-of-human-pathogens-in-produce-from-retail-markets-in-northern-germany
#9
Gregor Fiedler, Jan Kabisch, Christina Böhnlein, Melanie Huch, Biserka Becker, Gyu-Sung Cho, Charles M A P Franz
Two hundred fresh produce samples (cucumber, carrots, herbs, leaf lettuce, and ready-to-eat mixed salad leaves) were obtained from retail in northern Germany in 2015. These were investigated for microbial contamination and the presence of foodborne pathogens, including Listeria monocytogenes, Salmonella serovars, presumptive Bacillus (B.) cereus, and Shiga toxin-producing Escherichia coli using culture-dependent (enrichment, plating on selective media) and -independent (real-time polymerase chain reaction [PCR]) techniques...
June 8, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28594105/effects-of-cucumber-mosaic-virus-infected-chilli-plants-on-non-vector-bemisia-tabaci-hemiptera-aleyrodidae
#10
Khalid A Saad, Mohamad Roff M N, Rebecca H Hallett, Idris A B
Plant virus infections are known to alter host plant attractiveness and suitability for insect herbivores. This study was conducted to determine how Cucumber mosaic virus (CMV)-infected chilli plants affect the fitness and settling preferences of non-vector whitefly, Bemisia tabaci adults under dual-choice conditions with volatile organic compounds analyzed using solid phase micro-extraction coupled with gas chromatography-mass spectrometry (GC-MS). Results showed that the presence of CMV in chilli plants substantially affects the settling preferences of the B...
June 8, 2017: Insect Science
https://www.readbyqxmd.com/read/28592944/potential-reasons-for-prevalence-of-fusarium-wilt-in-oriental-melon-in-korea
#11
Yunhee Seo, Young Ho Kim
This study aims to examine the potential reasons for the current prevalence of the fusarium wilt in the oriental melon. Twenty-seven Fusarium isolates obtained from oriental melon greenhouses in 2010-2011 were identified morphologically and by analysis of elongation factor-1 alpha gene (EF-1α) and internal transcribed spacer (ITS) rDNA sequences as 6 Fusarium species (8 isolates of F. oxysporum, 8 F. commune, 5 F. proliferatum, 3 F. equiseti, 2 F. delphinoides, and 1 F. andiyazi), which were classified as same into 6 EF-1α sequence-based phylogenetic clades...
June 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28592854/transcriptomic-analysis-of-short-fruit-1-sf1-reveals-new-insights-into-the-variation-of-fruit-related-traits-in-cucumis-sativus
#12
Lina Wang, Chenxing Cao, Shuangshuang Zheng, Haiyang Zhang, Panjing Liu, Qian Ge, Jinrui Li, Zhonghai Ren
Fruit size is an important quality trait in different market classes of Cucumis sativus L., an economically important vegetable cultivated worldwide, but the genetic and molecular mechanisms that control fruit size are largely unknown. In this study, we isolated a natural cucumber mutant, short fruit 1 (sf1), caused by a single recessive Mendelian factor, from the North China-type inbred line CNS2. In addition to significantly decreased fruit length, other fruit-related phenotypic variations were also observed in sf1 compared to the wild-type (WT) phenotype, indicating that sf1 might have pleiotropic effects...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28590876/cucumber-green-mottle-mosaic-virus-rapidly-increasing-global-distribution-etiology-epidemiology-and-management
#13
Aviv Dombrovsky, Lucy T T Tran-Nguyen, Roger A C Jones
Cucumber green mottle mosaic virus (CGMMV) was first described in 1935 infecting cucumber, making it one of the first plant viruses to be described. Its initial distribution occurred out of England to other countries. This was followed by its subsequent distribution from England and these other countries. This process increased slowly between 1935 and 1985, faster between 1986 and 2006, and rapidly between 2007 and 2016. The discovery that it diminished cucurbit fruit yields and quality, especially of watermelon, prompted a substantial research effort in worst-affected countries...
June 7, 2017: Annual Review of Phytopathology
https://www.readbyqxmd.com/read/28587865/impact-of-vat-resistance-in-melon-on-viral-epidemics-and-genetic-structure-of-virus-populations
#14
Schoeny Alexandra, Desbiez Cécile, Millot Pauline, Wipf-Scheibel Catherine, Nozeran Karine, Gognalons Patrick, Lecoq Hervé, Boissot Nathalie
Cultivar choice is at the heart of cropping systems and resistant cultivars should be at the heart of disease management strategies whenever available. They are the easiest, most efficient and environmentally friendly way of combating viral diseases at the farm level. Among the melon genetic resources, Vat is a unique gene conferring resistance to both the melon aphid Aphis gossypii and the viruses it carries. The 'virus side' of this pleiotropic phenotype is seldom regarded as an asset for virus control. Indeed, the effect of Vat on virus epidemics in the field is expected to vary according to the composition of aphid populations in the environment and long-term studies are needed to draw a correct trend...
June 3, 2017: Virus Research
https://www.readbyqxmd.com/read/28583179/comparison-between-the-effects-of-potassium-phosphite-and-chitosan-on-changes-in-the-concentration-of-cucurbitacin-e-and-on-antibacterial-property-of-cucumis-sativus
#15
Moazzameh Ramezani, Fatemeh Rahmani, Ali Dehestani
BACKGROUND: Cucurbitacins are mostly found in the members of the family Cucurbitaceae and are responsible for the bitter taste of cucumber. Pharmacological activities such as anti-bacterial and anti-tumor effects have been attributed to these structurally divers triterpens. The aim of this study was to investigate the effect of potassium phosphite (KPhi) and chitosan on Cucurbitacin E (CuE) concentration in different tissues of Cucumis sativus. The antibacterial effect of plant ethanolic extracts was also examined against E...
June 5, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28583084/expression-and-functional-analysis-of-the-transcription-factor-encoding-gene-cserf004-in-cucumber-during-pseudoperonospora-cubensis-and-corynespora-cassiicola-infection
#16
Dong Liu, Ming Xin, Xiuyan Zhou, Chunhua Wang, Yanju Zhang, Zhiwei Qin
BACKGROUND: Cucumber downy mildew, caused by P. cubensis, is an important leaf disease that can severely affect cucumber production. In recent years, cucumber target spot, caused by C. cassiicola, has been reported in both Asia and Europe and is now considered as a major disease disrupting cucumber production. Single-disease-resistant cucumber varieties have been unable to satisfy production needs. To explore the molecular mechanisms of cucumber resistance to these two diseases, cucumber cultivars D9320 (resistant to downy mildew and target spot) and D0401 (susceptible to downy mildew and target spot) were used as experimental materials in this study...
June 5, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28580756/high-tunnels-protection-for-rather-than-from-insect-pests
#17
Laura L Ingwell, Sarah L Thompson, Ian Kaplan, Ricky E Foster
BACKGROUND: High tunnels are a season extension tool creating a hybrid of field and greenhouse growing conditions. High tunnels have recently increased in the U.S. and thus research on their management is lacking. One purported advantage of these structures is protection from common field pests, but evidence to support this claim is lacking. We compared insect pest populations in high tunnels with field production over two years on three crops: tomato, broccoli and cucumber. RESULTS: Greenhouse pests (e...
June 5, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28580151/transcriptomic-and-metabolomic-analyses-of-cucumber-fruit-peels-reveal-a-developmental-increase-in-terpenoid-glycosides-associated-with-age-related-resistance-to-phytophthora-capsici
#18
Ben N Mansfeld, Marivi Colle, Yunyan Kang, A Daniel Jones, Rebecca Grumet
The oomycete, Phytophthora capsici, infects cucumber (Cucumis sativus L.) fruit. An age-related resistance (ARR) to this pathogen was previously observed in fruit of cultivar 'Vlaspik' and shown to be associated with the peel. Young fruits are highly susceptible, but develop resistance at ~10-12 days post pollination (dpp). Peels from resistant (16 dpp) versus susceptible (8 dpp) age fruit are enriched with genes associated with defense, and methanolic extracts from resistant age peels inhibit pathogen growth...
2017: Horticulture Research
https://www.readbyqxmd.com/read/28572327/complete-genome-sequences-of-three-salmonella-enterica-subsp-enterica-serovar-saintpaul-isolates-associated-with-a-2013-multistate-outbreak-in-the-united-states
#19
Kuan Yao, Tim Muruvanda, Marc W Allard, Maria Hoffmann
In 2013, a multistate outbreak of Salmonella enterica subsp. enterica serovar Saintpaul from cucumber caused 84 cases of salmonellosis in the United States. In this announcement, we report the complete genome sequences of three clinical Salmonella Saintpaul isolates associated with the 2013 outbreak.
June 1, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28560847/importance-of-prumycin-produced-by-bacillus-amyloliquefaciens-sd-32-in-biocontrol-against-cucumber-powdery-mildew-disease
#20
Keijitsu Tanaka, Mutsumi Fukuda, Yusuke Amaki, Takatoshi Sakaguchi, Koji Inai, Atsushi Ishihara, Hiromitsu Nakajima
BACKGROUND: Powdery mildew disease of cucurbits is caused mainly by Podosphaera fusca, which is one of the most important limiting factors in cucurbit production worldwide. Previously we reported that Bacillus amyloliquefaciens biocontrol strain SD-32 produces C17 bacillomycin D and [Ile(7) ]surfactin, and that these metabolites play important roles in SD-32's biocontrol over cucumber gray mold disease. Our further investigation demonstrated that the culture broth and its supernatant suppressed cucumber powdery mildew disease in greenhouse experiments...
May 30, 2017: Pest Management Science
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