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https://www.readbyqxmd.com/read/28212329/enzyme-assisted-discovery-of-antioxidant-peptides-from-edible-marine-invertebrates-a-review
#1
REVIEW
Tsun-Thai Chai, Yew-Chye Law, Fai-Chu Wong, Se-Kwon Kim
Marine invertebrates, such as oysters, mussels, clams, scallop, jellyfishes, squids, prawns, sea cucumbers and sea squirts, are consumed as foods. These edible marine invertebrates are sources of potent bioactive peptides. The last two decades have seen a surge of interest in the discovery of antioxidant peptides from edible marine invertebrates. Enzymatic hydrolysis is an efficient strategy commonly used for releasing antioxidant peptides from food proteins. A growing number of antioxidant peptide sequences have been identified from the enzymatic hydrolysates of edible marine invertebrates...
February 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28198807/paenibacillus-polymyxa-nsy50-suppresses-fusarium-wilt-in-cucumbers-by-regulating-the-rhizospheric-microbial-community
#2
Lu Shi, Nanshan Du, Sheng Shu, Jin Sun, Shuzhan Li, Shirong Guo
Paenibacillus polymyxa (P. polymyxa) NSY50, isolated from vinegar residue substrate, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion. The aim of the present study was to evaluate the effects of NSY50 application on cucumber growth, soil properties and composition of the rhizospheric soil microbial community after exposure to Fusarium oxysporum. Bacterial and fungal communities were investigated by Illumina sequencing of the 16S rRNA gene and the internal transcribed spacer (ITS) regions (ITS1 and ITS2)...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28195398/preparation-of-magnetic-mesoporous-silica-composite-for-the-solid-phase-microextraction-of-diazinon-and-malathion-before-their-determination-by-high-performance-liquid-chromatography
#3
Alireza Bazmandegan-Shamili, Ali Mohammad Haji Shabani, Shayessteh Dadfarnia, Masoud Rohani Moghadam, Mahboubeh Saeidi
A novel magnetic mesoporous silica material was synthesized and used as the sorbent for the magnetic solid-phase microextraction of diazinon and malathion before their quantification by high-performance liquid chromatography with UV detection. The sorbent was synthesized by a surfactant-templated one-pot sol-gel procedure using SiO2 -coated Fe3 O4 as the magnetic support, cetyltrimethylammonium bromide as the template and tetraethyl orthosilicate as the silicon source. The characteristics of the prepared sorbent were investigated using FTIR spectroscopy, scanning electron microscopy, and X-ray diffraction...
February 13, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28194954/gecko-inspired-nanotentacle-surface-enhanced-raman-spectroscopy-substrate-for-sampling-and-reliable-detection-of-pesticide-residues-in-fruits-and-vegetables
#4
Pan Wang, Long Wu, Zhicheng Lu, Qin Li, Wenmin Yin, Fan Ding, Heyou Han
Rapid sampling and multicomponent detection are crucial for monitoring of pesticide residues analysis. Here, a gecko-inspired nanotentacle surface-enhanced Raman spectroscopy (G-SERS) platform is proposed for the first time for the simultaneous detection of three kinds of pesticides via a simple and intuitive "press and peeled-off" approach. The G-SERS platform obtained from seeding deposition of silver nanoparticles (Ag NPs) on 3D PDMS nanotentacle array is flexible and free-standing. Compared with other substrates, this G-SERS substrate can simultaneously provide outstanding SERS activity (enhancement factor = 1...
February 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28193581/genome-wide-characterization-and-expression-profiling-of-the-nac-genes-under-abiotic-stresses-in-cucumis-sativus
#5
Xiao Meng Zhang, Hong Jun Yu, Chao Sun, Jie Deng, Xue Zhang, Peng Liu, Yun Yun Li, Qiang Li, Wei Jie Jiang
The NAC (standing for no apical meristem [NAM], Arabidopsis transcription activation factor [ATAF] and cup-shaped cotyledon [CUC]) proteins pertain to one of the plant-specific transcription factor families that play important roles in plant development, abiotic stress resistance and signalling transduction. In the present study, the genomic features of the NAC genes in cucumber were analysed in depth using in silico tools. To reveal a tissue-specific, abiotic stress and hormone-responsive expression profile of CsNAC genes, RT-qPCR was performed under different treatments...
January 31, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28189057/itraq-reveals-proteomic-changes-during-intestine-regeneration-in-the-sea-cucumber-apostichopus-japonicus
#6
Lina Sun, Dongxue Xu, Qinzeng Xu, Jingchun Sun, Lili Xing, Libin Zhang, Hongsheng Yang
Sea cucumbers have a striking capacity to regenerate most of their viscera after evisceration, which has drawn the interest of many researchers. In this study, the isobaric tag for relative and absolute quantitation (iTRAQ) was utilized to investigate protein abundance changes during intestine regeneration in sea cucumbers. A total of 4073 proteins were identified, and 2321 proteins exhibited significantly differential expressions, with 1100 upregulated and 1221 downregulated proteins. Our results suggest that intestine regeneration constitutes a complex life activity regulated by the cooperation of various biological processes, including cytoskeletal changes, extracellular matrix (ECM) remodeling and ECM-receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism...
February 4, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28189056/metabolic-responses-to-intestine-regeneration-in-sea-cucumbers-apostichopus-japonicus
#7
Lina Sun, Jingchun Sun, Qinzeng Xu, Xiaoni Li, Libin Zhang, Hongsheng Yang
Sea cucumbers are excellent models for studying organ regeneration due to their striking capacity to regenerate most of their viscera after evisceration. In this study, we applied NMR-based metabolomics to determine the metabolite changes that occur during the process of intestine regeneration in sea cucumbers. Partial least-squares discriminant analysis showed that there was significant differences in metabolism between regenerative intestines at 3, 7, and 14days post evisceration (dpe) and normal intestines...
February 4, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28182745/prosystemin-overexpression-induces-transcriptional-modifications-of-defense-related-and-receptor-like-kinase-genes-and-reduces-the-susceptibility-to-cucumber-mosaic-virus-and-its-satellite-rnas-in-transgenic-tomato-plants
#8
Giovanni Bubici, Anna Vittoria Carluccio, Livia Stavolone, Fabrizio Cillo
Systemin is a plant signal peptide hormone involved in the responses to wounding and insect damage in the Solanaceae family. It works in the same signaling pathway of jasmonic acid (JA) and enhances the expression of proteinase inhibitors. With the aim of studying a role for systemin in plant antiviral responses, a tomato (Solanum lycopersicum) transgenic line overexpressing the prosystemin cDNA, i.e. the systemin precursor, was inoculated with Cucumber mosaic virus (CMV) strain Fny supporting either a necrogenic or a non-necrogenic satellite RNA (satRNA) variant...
2017: PloS One
https://www.readbyqxmd.com/read/28178932/variability-among-cucurbitaceae-species-melon-cucumber-and-watermelon-in-a-genomic-region-containing-a-cluster-of-nbs-lrr-genes
#9
Jordi Morata, Pere Puigdomènech
BACKGROUND: Cucurbitaceae species contain a significantly lower number of genes coding for proteins with similarity to plant resistance genes belonging to the NBS-LRR family than other plant species of similar genome size. A large proportion of these genes are organized in clusters that appear to be hotspots of variability. The genomes of the Cucurbitaceae species measured until now are intermediate in size (between 350 and 450 Mb) and they apparently have not undergone any genome duplications beside those at the origin of eudicots...
February 8, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28176159/new-korean-isolates-of-pepper-mild-mottle-virus-pmmov-differ-in-symptom-severity-and-subcellular-localization-of-the-126%C3%A2-kda-protein
#10
Sang-Hyuk Han, Jong-Seo Park, Jae-Yeong Han, Jun-Su Gong, Chan-Hwan Park, Jung-Kyu Kim, Eun-Young Seo, Leslie L Domier, John Hammond, Hyoun-Sub Lim
Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76...
February 7, 2017: Virus Genes
https://www.readbyqxmd.com/read/28166302/the-influence-of-different-concentrations-of-bio-organic-fertilizer-on-cucumber-fusarium-wilt-and-soil-microflora-alterations
#11
Nan Huang, Weiwei Wang, Yanlai Yao, Fengxiang Zhu, Weiping Wang, Xiaojuan Chang
Fusarium wilt is one of the main diseases of cucumber, and bio-organic fertilizer has been used to control Fusarium wilt. In this study, a pot experiment was conducted to evaluate the effects of bio-organic fertilizer applied at four levels on the suppression of Fusarium wilt disease in cucumber, the soil physico-chemical properties and the microbial communities. In comparison with the control (CK), low concentrations of bio-organic fertilizer (BIO2.5 and BIO5) did not effectively reduce the disease incidence and had little effect on soil microorganisms...
2017: PloS One
https://www.readbyqxmd.com/read/28161535/4-o-sulfation-in-sea-cucumber-fucodians-contribute-to-reversing-dyslipidiaemia-caused-by-hfd
#12
Shan Li, Junhui Li, Zijian Zhi, Yaqin Hu, Jian Ge, Xingqian Ye, Ding Tian, Linhardt Robert, Shiguo Chen
Fucodians extracted from sea cucumbers (SC-FUCs) possess linear chains with uniform repeating units. Sulfation patterns endow SC-FUCs unique structures and bioactivity. The present study investigated the anti-hyperlipidemic activity of two fucodians isolated from sea cucumbers Pearsonothuria graeffei (fuc-Pg) and Isostichopus badionotus (fuc-Ib). The results indicate fuc-Pg dominated with a 4-O-sulfation pattern shows strong activity in reducing body weight, regulating lipid disorder (TC, TG, HLD-C, and LDL-C level), improving liver function (liver weight, GOP, GPT, and TBA concentrations) and increasing adiponectin level (adiponectin concentration) caused by HFD...
February 1, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28160609/understanding-regulation-of-micrornas-on-intestine-regeneration-in-the-sea-cucumber-apostichopus-japonicus-using-high-throughput-sequencing
#13
Lina Sun, Jingchun Sun, Xiaoni Li, Libin Zhang, Hongsheng Yang, Qing Wang
The sea cucumber, as a member of the Echinodermata, has the capacity to restore damaged organs and body parts, which has always been a key scientific issue. MicroRNAs (miRNAs), a class of short noncoding RNAs, play important roles in regulating gene expression. In the present study, we applied high-throughput sequencing to investigate alterations of miRNA expression in regenerative intestine compared to normal intestine. A total of 73 differentially expressed miRNAs were obtained, including 59 up-regulated miRNAs and 14 down-regulated miRNAs...
January 25, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/28154572/transcriptomic-analysis-implies-that-ga-regulates-sex-expression-via-ethylene-dependent-and-ethylene-independent-pathways-in-cucumber-cucumis-sativus-l
#14
Yan Zhang, Guiye Zhao, Yushun Li, Ning Mo, Jie Zhang, Yan Liang
Sex differentiation of flower buds is an important developmental process that directly affects fruit yield of cucumber (Cucumis sativus L.). Plant hormones, such as gibberellins (GAs) and ethylene can promote development of male and female flowers, respectively, however, the regulatory mechanisms of GA-induced male flower formation and potential involvement of ethylene in this process still remain unknown. In this study, to unravel the genes and gene networks involved in GA-regulated cucumber sexual development, we performed high throughout RNA-Seq analyses that compared the transcriptomes of shoot tips between GA3 treated and untreated gynoecious cucumber plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28153461/purification-structural-characterization-and-anticoagulant-properties-of-fucosylated-chondroitin-sulfate-isolated-from-holothuria-mexicana
#15
Jiaojiao Mou, Cong Wang, Wenjing Li, Jie Yang
A novel fucosylated chondroitin sulfate (HmG) was isolated from sea cucumber Holothuria mexicana, the structure of which was characterized by monosaccharide composition, disaccharide composition, IR, (1)H and (13)C NMR spectrum, additionally with two dimensional NMR spectrum of degraded HmG (DHmG). The backbone of HmG was identified as chondroitin 6-O sulfate, while the major O-4 sulfated fucose branches linked to O-3 position of glucuronic acid in almost every disaccharide unit. The anticoagulant activities of HmG and DHmG were assessed and compared with heparin and low molecular weight heparin...
January 30, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28148297/transcriptome-analysis-of-cucumis-sativus-infected-by-cucurbit-chlorotic-yellows-virus
#16
Xinyan Sun, Zhenyue Wang, Qinsheng Gu, Honglian Li, Weili Han, Yan Shi
BACKGROUND: Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus-host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis. METHODS: We used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection. RESULTS: CCYV infection resulted in 865 DEGs...
February 2, 2017: Virology Journal
https://www.readbyqxmd.com/read/28146587/the-morphogenesis-related-ndr-kinase-pathway-of-colletotrichum-orbiculare-is-required-for-translating-plant-surface-signals-into-infection-related-morphogenesis-and-pathogenesis
#17
Sayo Kodama, Junya Ishizuka, Ito Miyashita, Takaaki Ishii, Takumi Nishiuchi, Hideto Miyoshi, Yasuyuki Kubo
Plant infection by pathogenic fungi involves the differentiation of appressoria, specialized infection structures, initiated by fungal sensing and responding to plant surface signals. How plant fungal pathogens control infection-related morphogenesis in response to plant-derived signals has been unclear. Here we showed that the morphogenesis-related NDR kinase pathway (MOR) of the cucumber anthracnose fungus Colletotrichum orbiculare is crucial for appressorium development following perception of plant-derived signals...
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28137747/2b-or-not-2b-experimental-evolution-of-functional-exogenous-sequences-in-a-plant-rna-virus
#18
Anouk Willemsen, Mark P Zwart, Silvia Ambrós, José L Carrasco, Santiago F Elena
Horizontal gene transfer (HGT) is pervasive in viruses, and thought to be a key mechanism in their evolution. On the other hand, strong selective constraints against increasing genome size are an impediment for HGT, rapidly purging horizontally transferred sequences and thereby potentially hindering evolutionary innovation. Here we explore experimentally the evolutionary fate of viruses with simulated HGT events, using the plant RNA virus Tobacco etch virus (TEV), by separately introducing two functional, exogenous sequences to its genome...
January 30, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28134423/tobacco-chewing-and-risk-of-gastric-cancer-a-case-control-study-in-yemen
#19
F A Al-Qadasi, S A Shah, H F Ghazi
This study aimed to assess the risk factors for gastric cancer in Yemen. A hospital-based case-control study of 70 cases and 140 controls was carried out in Sana'a city between May and October 2014. A structured questionnaire was used to collect information through direct interview. Living in rural areas, tobacco chewing and drinking untreated water were significant risk factors for gastric cancer. Frequent consumption of chicken, cheese, milk, starchy vegetables, cucumber, carrots, leeks, sweet pepper, fruit drinks, legumes and olive oil were associated significantly with decreased risk of gastric cancer...
January 23, 2017: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
https://www.readbyqxmd.com/read/28127253/frequency-of-a-natural-truncated-allele-of-mdmlo19-in-the-germplasm-of-malus-domestica
#20
Stefano Pessina, Luisa Palmieri, Luca Bianco, Jennifer Gassmann, Eric van de Weg, Richard G F Visser, Pierluigi Magnago, Henk J Schouten, Yuling Bai, R Riccardo Velasco, Mickael Malnoy
Podosphaera leucotricha is the causal agent of powdery mildew (PM) in apple. To reduce the amount of fungicides required to control this pathogen, the development of resistant apple cultivars should become a priority. Resistance to PM was achieved in various crops by knocking out specific members of the MLO gene family that are responsible for PM susceptibility (S-genes). In apple, the knockdown of MdMLO19 resulted in PM resistance. However, since gene silencing technologies such as RNAi are perceived unfavorably in Europe, a different approach that exploits this type of resistance is needed...
2017: Molecular Breeding: New Strategies in Plant Improvement
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