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Cryobiology

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https://www.readbyqxmd.com/read/29417894/strong-heterogeneity-in-advances-in-cryopreservation-techniques-in-the-mammalian-orders
#1
Sebastian J Charlton, Mikkel B Nielsen, Carina R Pedersen, Lisette Thomsen, Mads P Kristjansen, Thomas B Sørensen, Cino Pertoldi, Julie Strand
Between 1970 and 2012, vertebrate abundance has declined by 58% with an average annual decline of 2%, calling for serious action to prevent a mass extinction and an irreversible loss of biodiversity. Cryobanks and cryopreservation have the potential to assist and improve ex situ and in situ conservation strategies by storing valuable genetic material. A great deal of studies concerning cryopreservation have been performed within the class Mammalia, although no systematic overview has previously been presented...
February 2018: Zoological Science
https://www.readbyqxmd.com/read/29327217/cryobiotechnology-of-apple-malus-spp-development-progress-and-future-prospects
#2
REVIEW
Min-Rui Wang, Long Chen, Jaime A Teixeira da Silva, Gayle M Volk, Qiao-Chun Wang
Cryopreservation provides valuable genes for further breeding of elite cultivars, and cryotherapy improves the production of virus-free plants in Malus spp., thus assisting the sustainable development of the apple industry. Apple (Malus spp.) is one of the most economically important temperate fruit crops. Wild Malus genetic resources and existing cultivars provide valuable genes for breeding new elite cultivars and rootstocks through traditional and biotechnological breeding programs. These valuable genes include those resistant to abiotic factors such as drought and salinity, and to biotic factors such as fungi, bacteria and aphids...
January 11, 2018: Plant Cell Reports
https://www.readbyqxmd.com/read/29258708/a-simple-flow-cytometry-protocol-to-determine-simultaneously-live-dead-and-apoptotic-stallion-spermatozoa-in-fresh-and-frozen-thawed-samples
#3
M C Gil, C Ortega Ferrusola, L Anel-López, J M Ortiz-Rodriguez, M Alvarez, P de Paz, L Anel, F J Peña
Spermatozoa undergo apoptotic changes during the cryopreservation process. These changes, recently termed spermptosis, resemble the cryopreservation induced delayed onset of cell death observed after thawing of somatic cells. Due to its importance in cryobiology, methods to easily identify spermptotic cells are warranted. In this study, a well-validated method for identification of spermatozoa with caspase 3 activity was compared with use of the combination of Hoechst 33342 (H-42) and ethidium homodimer (Eth-1)...
December 16, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29197430/corrigendum-to-methodology-for-reliable-and-reproducible-cryopreservation-of-human-cervical-tissue-cryobiology-77-august-2017-14-18
#4
James M Fox, Rebecca C Wiggins, John W J Moore, Christine Brewer, Bill Hunter, Alison C Andrew, Fabiola Martin
No abstract text is available yet for this article.
December 2017: Cryobiology
https://www.readbyqxmd.com/read/29115839/nonideal-solute-chemical-potential-equation-and-the-validity-of-the-grouped-solute-approach-for-intracellular-solution-thermodynamics
#5
Michal W Zielinski, Locksley E McGann, John A Nychka, Janet A W Elliott
The prediction of nonideal chemical potentials in aqueous solutions is important in fields such as cryobiology, where models of water and solute transport-that is, osmotic transport-are used to help develop cryopreservation protocols and where solutions contain many varied solutes and are generally highly concentrated and thus thermodynamically nonideal. In this work, we further the development of a nonideal multisolute solution theory that has found application across a broad range of aqueous systems. This theory is based on the osmotic virial equation and does not depend on multisolute data...
November 8, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29052330/aquaporins-in-the-male-reproductive-tract-and-sperm-functional-implications-and-cryobiology
#6
REVIEW
M Yeste, R Morató, J E Rodríguez-Gil, S Bonet, N Prieto-Martínez
Aquaporins (AQPs) play a vital role for the transport of water and solutes across cell membranes. Classification of these ubiquitous proteins into three categories (orthodox AQPs, aquaglyceroporins and superaquaporins) is based on their sequence similarity and substrate selectivity. In the male reproductive tract of mammals, most AQPs (except AQP6 and AQP12) are found in different organs (including testis, efferent ducts and epididymis). AQP1 and AQP9 are the most abundant AQPs in the efferent ducts and epididymis and play a crucial role for the secretion/reabsorption dynamics of luminal fluid during sperm transport and maturation...
October 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28961002/thermodynamic-investigation-of-the-effect-of-interface-curvature-on-the-solid-liquid-equilibrium-and-eutectic-point-of-binary-mixtures
#7
Fanghui Liu, Leila Zargarzadeh, Hyun-Joong Chung, Janet A W Elliott
Thermodynamic phase behavior is affected by curved interfaces in micro- and nanoscale systems. For example, capillary freezing point depression is associated with the pressure difference between the solid and liquid phases caused by interface curvature. In this study, the thermal, mechanical, and chemical equilibrium conditions are derived for binary solid-liquid equilibrium with a curved solid-liquid interface due to confinement in a capillary. This derivation shows the equivalence of the most general forms of the Gibbs-Thomson and Ostwald-Freundlich equations...
September 29, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28956627/cryobiology-meets-biobanking-in-hefei-china
#8
Dayong Gao, Zisis Kozlakidis, Brent Schacter, Jim Vaught
No abstract text is available yet for this article.
September 28, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28814236/neuroglial-cell-cryopreservation-in-appropriate-inductive-conditions
#9
Rosa M Gomez, Maria Andrea Dominguez-Sanchez, Magdy M Sanchez, George E Barreto
An ability to store cells (if they cannot be used fresh) reduces cell wastage, thereby increasing the supply of transplantable material. Cell storage is also valuable in scientific research, allowing material to be archived and experiments to be repeated from the same tissue source and facilitating research collaboration by allowing stored samples to be distributed. Cryopreservation is currently considered the most promising and successful, long-term biological conservation method. Its use has led to optimizing survival, improving protocols and stem cell and neuroglia viability, thereby assuring its future use in neuroregeneration and restoration regarding cell therapy...
August 16, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28510115/application-of-decellularized-tissue-scaffolds-in-ovarian-tissue-transplantation
#10
Enes Taylan, Kutluk Oktay
In tissue engineering, decellularized scaffolds have been proved to have remarkable capacity to promote regeneration in various organs such as kidney, heart, lung, and liver. Marrying the field of cryobiology and reproductive medicine resulted in considerable progress and breakthroughs, which led to the emergence of ovarian tissue cryopreservation and transplantation as a promising option for fertility preservation. Here we describe an innovative application of decellularized tissue scaffolds as a regenerative platform for reconstruction of ovarian grafts for auto-transplantation...
May 17, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28428046/cryoprotectants-a-review-of-the-actions-and-applications-of-cryoprotective-solutes-that-modulate-cell-recovery-from-ultra-low-temperatures
#11
REVIEW
Gloria D Elliott, Shangping Wang, Barry J Fuller
Cryopreservation has become a central technology in many areas of clinical medicine, biotechnology, and species conservation within both plant and animal biology. Cryoprotective agents (CPAs) invariably play key roles in allowing cells to be processed for storage at deep cryogenic temperatures and to be recovered with high levels of appropriate functionality. As such, these CPA solutes possess a wide range of metabolic and biophysical effects that are both necessary for their modes of action, and potentially complicating for cell biological function...
April 18, 2017: Cryobiology
https://www.readbyqxmd.com/read/28421508/appendix-d-irvine-scientific-%C3%A2-vitrification-system
#12
Matthew VerMilyea, Amber Brewer
This chapter will describe the use of the Irvine Scientific(®) vitrification and warming solutions (Vit Kits(®)) along with a detailed protocol for the correct use of the CryoTip(®) vitrification device for human embryos and oocytes. Successful pregnancies have been reported after carrying out rapid vitrification methods of oocytes, cleavage stage embryos, and blastocysts using the CryoTip(®) (Popwell et al. Fertil Steril, 101:e20, 2014; Kuwayama et al. Reprod Biomed 11:608-614, 2005; Kuwayama et al. Fertil Steril 84:S187, 2005; Kuwayama et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28421501/chapter-17-sterile-plate-based-vitrification-of-adherent-human-pluripotent-stem-cells-and-their-derivatives-using-the-twist-method
#13
Julia C Neubauer, Frank Stracke, Heiko Zimmermann
Due to their high biological complexity, e.g., their close cell-to-cell contacts, cryopreservation of human pluripotent stem cells with standard slow-rate protocols often is inefficient and can hardly be standardized. Vitrification that means ultrafast freezing already showed very good viability and recovery rates for this sensitive cell system, but is only applicable for low cell numbers, bears a high risk of contamination, and can hardly be implemented under GxP regulations. In this chapter, a sterile plate-based vitrification method for adherent pluripotent stem cells and their derivatives is presented based on a procedure and device for human embryonic stem cells developed by Beier et al...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28315320/controlled-ice-nucleation-using-freeze-dried-pseudomonas-syringae-encapsulated-in-alginate-beads
#14
Lindong Weng, Shannon N Tessier, Anisa Swei, Shannon L Stott, Mehmet Toner
The control of ice nucleation is of fundamental significance in many process technologies related to food and pharmaceutical science and cryobiology. Mechanical perturbation, electromagnetic fields and ice-nucleating agents (INAs) have been known to induce ice nucleation in a controlled manner. But these ice-nucleating methods may suffer from cumbersome manual operations, safety concerns of external fields, and biocompatibility and recovery issues of INA particles, especially when used in living systems. Given the automatic ice-seeding nature of INAs, a promising solution to overcome some of the above limitations is to engineer a biocomposite that accommodates the INA particles but minimizes their interactions with biologics, as well as enabling the recovery of used particles...
April 2017: Cryobiology
https://www.readbyqxmd.com/read/28150589/effects-of-heat-and-freeze-on-isolated-erythrocyte-submembrane-skeletons
#15
Ivan T Ivanov, Boyana K Paarvanova, Veselin Ivanov, Kathrin Smuda, Hans Bäumler, Radostina Georgieva
In this study we heated insoluble residues, obtained after Triton-X-100 (0.1 v/v%) extraction of erythrocyte ghost membranes (EGMs). Specific heat capacity, electric capacitance and resistance, and optical transmittance (280 nm) sustained sharp changes at 49°C (TA) and 66°C (TC), the known denaturation temperatures of spectrin and band 3, respectively. The change at TA was selectively inhibited by diamide (1 mM) and taurine mustard (1 mM) while its inducing temperature was selectively decreased by formamide in full concert with the assumed involvement of spectrin denaturation...
April 2017: General Physiology and Biophysics
https://www.readbyqxmd.com/read/27951652/multivalent-display-of-antifreeze-proteins-by-fusion-to-self-assembling-protein-cages-enhances-ice-binding-activities
#16
Sean W Phippen, Corey A Stevens, Tyler D R Vance, Neil P King, David Baker, Peter L Davies
Antifreeze proteins (AFPs) are small monomeric proteins that adsorb to the surface of ice to inhibit ice crystal growth and impart freeze resistance to the organisms producing them. Previously, monomeric AFPs have been conjugated to the termini of branched polymers to increase their activity through the simultaneous binding of more than one AFP to ice. Here, we describe a superior approach to increasing AFP activity through oligomerization that eliminates the need for conjugation reactions with varying levels of efficiency...
December 13, 2016: Biochemistry
https://www.readbyqxmd.com/read/27916562/cryobiological-parameters-of-multipotent-stromal-cells-obtained-from-different-sources
#17
L Lauterboeck, W F Wolkers, B Glasmacher
Stem cells are important for regenerative medicine mainly due to their multilineage differentiation capacity. However, the cells rapidly loose this capability during culturing. Cryopreservation preserves the differentiation potential of the cells, until they are needed. In this study, specific cell properties of multipotent stromal cells (MSCs), from the common marmoset monkey Callithrix jacchus MSCs derived from amnion (Am) and bone marrow (Bm) were studied in order to predict optimal cooling rates for cryopreservation...
February 2017: Cryobiology
https://www.readbyqxmd.com/read/27769741/three-dimensional-printing-with-polylactic-acid-pla-thermoplastic-offers-new-opportunities-for-cryobiology
#18
Terrence R Tiersch, William T Monroe
Development of devices through design, prototyping, testing, and fabrication is especially necessary for enhancement of research and eventual application in cryobiology. The advent of 3-dimensional printing offers unique opportunities for this process, given that the materials involved are suitable for use in cryogenic temperatures. We report herein that 3-D printing with polylactic acid (PLA) thermoplastic is ideally suited for cryobiology device development. Devices that are designed and standardized in open-source fashion can be electronically distributed and created locally on increasingly affordable 3-D printers, and can accelerate cryobiology findings and improve reproducibility of results...
December 2016: Cryobiology
https://www.readbyqxmd.com/read/27603179/xeno-free-cryopreservation-of-bone-marrow-derived-multipotent-stromal-cells-from-callithrix-jacchus
#19
Lothar Lauterboeck, Debapriya Saha, Anamika Chatterjee, Nicola Hofmann, Birgit Glasmacher
In the previous decade, numerous biobanks were established and have created large markets for the storage of bioactive compounds, cells, and tissues for medical and diagnostic applications. For in vivo clinical and therapeutic purposes, it is critical to use well-defined and xeno-free components during cultivation, preservation, and transplantation of biological material. Safe and efficacious storage of bioactive molecules, cells, and tissues, without the addition of undefined medium components, minimizes risks of zoonotic disease transmission and is thus an essential and desirable prerequisite for biobanks...
December 2016: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/27597869/recent-advances-and-future-direction-in-lyophilisation-and-desiccation-of-mesenchymal-stem-cells
#20
REVIEW
Akalabya Bissoyi, Awanish Kumar, Albert A Rizvanov, Alexander Nesmelov, Oleg Gusev, Pradeep Kumar Patra, Arindam Bit
Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context...
2016: Stem Cells International
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