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Lothar Lauterboeck, Debapriya Saha, Anamika Chatterjee, Nicola Hofmann, Birgit Glasmacher
In the previous decade, numerous biobanks were established and have created large markets for the storage of bioactive compounds, cells, and tissues for medical and diagnostic applications. For in vivo clinical and therapeutic purposes, it is critical to use well-defined and xeno-free components during cultivation, preservation, and transplantation of biological material. Safe and efficacious storage of bioactive molecules, cells, and tissues, without the addition of undefined medium components, minimizes risks of zoonotic disease transmission and is thus an essential and desirable prerequisite for biobanks...
September 7, 2016: Biopreservation and Biobanking
Akalabya Bissoyi, Awanish Kumar, Albert A Rizvanov, Alexander Nesmelov, Oleg Gusev, Pradeep Kumar Patra, Arindam Bit
Mesenchymal Stem Cells (MSCs) are a promising mammalian cell type as they can be used for the reconstruction of human tissues and organs. MSCs are shown to form bone, cartilage, fat, and muscle-like cells under specific cultivation conditions. Current technology of MSCs cryopreservation has significant disadvantages. Alternative technologies of mammalian cells preservation through lyophilisation or desiccation (air-drying) are among the upcoming domains of investigation in the field of cryobiology. Different protectants and their combinations were studied in this context...
2016: Stem Cells International
Lu Zhang, Xu Xue, Jie Yan, Li-Ying Yan, Xiao-Hu Jin, Xiao-Hui Zhu, Zhi-Zhu He, Jing Liu, Rong Li, Jie Qiao
BACKGROUND: L-proline is a natural, nontoxic cryoprotectant that helps cells and tissues to tolerate freezing in a variety of plants and animals. The use of L-proline in mammalian oocyte cryopreservation is rare. In this study, we explored the cryobiological characteristics of L-proline and evaluated its protective effect in mouse oocyte cryopreservation. METHODS: The freezing property of L-proline was detected by Raman spectroscopy and osmometer. Mature oocytes obtained from 8-week-old B6D2F1 mice were vitrified in a solution consisting various concentration of L-proline with a reduced proportion of dimethyl sulfoxide (DMSO) and ethylene glycol (EG), comparing with the control group (15% DMSO and 15% EG without L-proline)...
August 20, 2016: Chinese Medical Journal
Lindong Weng, Shannon N Tessier, Kyle Smith, Jon F Edd, Shannon L Stott, Mehmet Toner
Ice nucleation is of fundamental significance in many areas, including atmospheric science, food technology, and cryobiology. In this study, we investigated the ice-nucleation characteristics of picoliter-sized drops consisting of different D2O and H2O mixtures with and without the ice-nucleating bacteria Pseudomonas syringae. We also studied the effects of commonly used cryoprotectants such as ethylene glycol, propylene glycol, and trehalose on the nucleation characteristics of D2O and H2O mixtures. The results show that the median freezing temperature of the suspension containing 1 mg/mL of a lyophilized preparation of P...
September 13, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Lili E Ehrlich, Jonathan A Malen, Yoed Rabin
The thermal conductivity of the cryoprotective agent (CPA) cocktail DP6 in combination with synthetic ice modulators (SIMs) is measured in this study, using a transient hot-wire method. DP6 is a mixture of 3 M dimethyl sulfoxide (DMSO) and 3 M propylene glycol, which received significant attention in the cryobiology community in recent years. Tested SIMs include 6% 1,3Cyclohexanediol, 6% 2,3Butanediol, and 12% PEG400 (percentage by volume). This study integrates the scanning cryomacroscope for visual verification of crystallization and vitrification events...
October 2016: Cryobiology
Justin S G Feig, Prem K Solanki, David P Eisenberg, Yoed Rabin
This study aims at developing thermal analysis tools and explaining experimental observations made by means of polarized-light cryomacroscopy (Part I). Thermal modeling is based on finite elements analysis (FEA), where two model parameters are extracted from thermal measurements: (i) the overall heat transfer coefficient between the cuvette and the cooling chamber, and (ii) the effective thermal conductivity within the cryoprotective agent (CPA) at the upper part of the cryogenic temperature range. The effective thermal conductivity takes into account enhanced heat transfer due to convection currents within the CPA, creating the so-called Bénard cells...
October 2016: Cryobiology
Erik J Woods, Sreedhar Thirumala, Sandhya S Badhe-Buchanan, Dominic Clarke, Aby J Mathew
The field of cellular therapeutics has immense potential, affording an exciting array of applications in unmet medical needs. One of several key issues is an emphasis on getting these therapies from bench to bedside without compromising safety and efficacy. The successful commercialization of cellular therapeutics will require many to extend the shelf-life of these therapies beyond shipping "fresh" at ambient or chilled temperatures for "just in time" infusion. Cryopreservation is an attractive option and offers potential advantages, such as storing and retaining patient samples in case of a relapse, banking large quantities of allogeneic cells for broader distribution and use and retaining testing samples for leukocyte antigen typing and matching...
June 2016: Cytotherapy
Stuart A Lavery, Rumana Islam, Jennifer Hunt, Anna Carby, Richard A Anderson
Cryopreservation of oocytes has been proposed as a way of storing gametes in young patients at high risk of infertility and premature ovarian failure. Recent advances in cryobiology have yielded promising results, leading to oocyte cryopreservation becoming a mainstay of fertility preservation. In this case series, we describe the feasibility of performing ovarian stimulation, and the ethical challenges faced, in teenage girls, aged 14-18 years, prior to undergoing bone marrow transplant for sickle cell anaemia...
July 2016: Human Reproduction
Arpa Hudait, Siwei Qiu, Laura Lupi, Valeria Molinero
Crystallization of ice from deeply supercooled water and amorphous ices - a process of fundamental importance in the atmosphere, interstellar space, and cryobiology - results in stacking disordered ices with a wide range of metastabilities with respect to hexagonal ice. The structural origin of this high variability, however, has not yet been elucidated. Here we use molecular dynamics simulations with the mW water model to characterize the structure of ice freshly grown from supercooled water at temperatures from 210 to 270 K, the thermodynamics of stacking faults, line defects, and interfaces, and to elucidate the interplay between kinetics and thermodynamics in determining the structure of ice...
April 14, 2016: Physical Chemistry Chemical Physics: PCCP
Zhiquan Shu, Sean M Hughes, Cifeng Fang, Jinghua Huang, Baiwen Fu, Gang Zhao, Michael Fialkow, Gretchen Lentz, Florian Hladik, Dayong Gao
Cryopreservation of specimens taken from the genital tract of women is important for studying mucosal immunity during HIV prevention trials. However, it is unclear whether the current, empirically developed cryopreservation procedures for peripheral blood cells are also ideal for genital specimens. The optimal cryopreservation protocol depends on the cryobiological features of the cells. Thus, we obtained tissue specimens from vaginal repair surgeries, isolated and flow cytometry-purified immune cells, and determined fundamental cryobiological characteristics of vaginal CD3(+) T cells and CD14(+) macrophages using a microfluidic device...
April 2016: Cryobiology
Sayyed Morteza Hosseini, Mohammad Hossein Nasr-Esfahani
In October 2012, the American Society for Reproductive Medicine (ASRM) and, in March 2012, the European Society of Human Reproduction and Embryology (ESHRE), lifted the categorization of oocyte cryopreservation as being "experimental" and endorsed its entrance into the mainstream of assisted reproductive techniques. This change in policy, with the considerable advantages that oocytes offer over embryos for cryopreservation, has increased applications of oocyte cryopreservation in assisted reproduction techniques...
April 2016: Reproductive Biomedicine Online
Amir Arav, Joseph Saragusty
Directional freezing has now completed 30 years of development since it was first introduced to cryobiology. In the field of sperm cryopreservation, directional freezing has been shown to be advantageous over slow freezing for numerous domestic and wildlife species. In particular, it was shown that freezing of large volume is possible. Furthermore, double freezing of sperm and freezing of sex-sorted sperm are possible and became the routine in the sex sorted sperm industry. In wild animals, our labs and others showed that sperm from a wide range of terrestrial and aquatic species can be successfully cryopreserved using directional freezing...
June 2016: Animal Reproduction Science
Dan Niu, Gang Zhao, Xiaoli Liu, Ping Zhou, Yunxia Cao
High-survival-rate cryopreservation of endothelial cells plays a critical role in vascular tissue engineering, while optimization of osmotic injuries is the first step toward successful cryopreservation. We designed a low-cost, easy-to-use, microfluidics-based microperfusion chamber to investigate the osmotic responses of human umbilical vein endothelial cells (HUVECs) at different temperatures, and then optimized the protocols for using cryoprotective agents (CPAs) to minimize osmotic injuries and improve processes before freezing and after thawing...
March 2016: Tissue Engineering. Part C, Methods
L Zalazar, A Ledesma, F Hozbor, A Cesari
During the last decades fundamental and applied aspects of mammalian ram sperm cryopreservation have been increasingly explored by scientists and biotechnologists. Many works report modifications in the composition of the freezing extenders and explore the beneficial and detrimental effects of seminal plasma or seminal plasma components in cryopreservation. Seminal plasma is known to contain stabilizing proteins, thereby this is a good start point to study the maintenance of membrane stability based on the basic knowledge of sperm physiology...
January 2016: Animal Reproduction Science
Silvia Landi, Elisabetta Gargani, Francesco Paoli, Sauro Simoni, Pio Federico Roversi
Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) is an invasive pest recently reported in Europe whose spread into new areas has caused severe economic damage to many agricultural crops. There are serious concerns about the currently available chemical insecticides because of their low efficacy in controlling the species and their environmental impact; so, several studies have focused on environmentally safe strategies. The sterile insect technique (SIT), which requires colony maintenance in laboratory and production of large numbers of live animals, can be utilized in pest management programs and could be integrated with other control strategies if the potential risks associated with the rearing and maintenance of the insect line under laboratory conditions are given sufficient attention...
August 2015: Journal of Economic Entomology
Robert L McIntyre, Gregory M Fahy
We describe here a new cryobiological and neurobiological technique, aldehyde-stabilized cryopreservation (ASC), which demonstrates the relevance and utility of advanced cryopreservation science for the neurobiological research community. ASC is a new brain-banking technique designed to facilitate neuroanatomic research such as connectomics research, and has the unique ability to combine stable long term ice-free sample storage with excellent anatomical resolution. To demonstrate the feasibility of ASC, we perfuse-fixed rabbit and pig brains with a glutaraldehyde-based fixative, then slowly perfused increasing concentrations of ethylene glycol over several hours in a manner similar to techniques used for whole organ cryopreservation...
December 2015: Cryobiology
Hossein Vaseghi Dodaran, Mahdi Zhandi, Mohsen Sharafi, Elaheh Nejati-Amiri, Ardeshir Nejati-Javaremi, Abdollah Mohammadi-Sangcheshmeh, Mohamed Ahmed Mohamed Mahmoud Shehab-El-Deen, Malak Shakeri
No abstract text is available yet for this article.
September 23, 2015: Cryobiology
Anthony J F Reardon, Janet A W Elliott, Locksley E McGann
The success of cryopreservation protocols is largely based on membrane integrity assessments after thawing, since membrane integrity can be considered to give an upper limit in assessment of cell viability and the plasma membrane is considered to be a primary site of cryoinjury. However, the exposure of cells to conditions associated with low temperatures can induce injury to cellular structure and function that may not be readily identified by membrane integrity alone. Interrupted cooling protocols (including interrupted slow cooling without a hold time (graded freezing), and interrupted rapid cooling with a hold time (two-step freezing)), can yield important information about cryoinjury by separating the damage that occurs upon cooling to (and possibly holding at) a critical intermediate temperature range from the damage that occurs upon plunging to the storage temperature (liquid nitrogen)...
October 2015: Cryobiology
Lili E Ehrlich, Justin S G Feig, Scott N Schiffres, Jonathan A Malen, Yoed Rabin
Thermal conductivity of dimethyl-sulfoxide (DMSO) solution is measured in this study using a transient hot wire technique, where DMSO is a key ingredient in many cryoprotective agent (CPA) cocktails. Characterization of thermal properties of cryoprotective agents is essential to the analysis of cryopreservation processes, either when evaluating experimental data or for the design of new protocols. Also presented are reference measurements of thermal conductivity for pure water ice and glycerol. The thermal conductivity measurement setup is integrated into the experimentation stage of a scanning cryomacroscope apparatus, which facilitates the correlation of measured data with visualization of physical events...
2015: PloS One
K B Aslanidi
The microspectral analysis technique was used to identify molecular components of a number of plant and animal cells. This technique allows of performing optical measurements during freezing down to the temperature of liquid nitrogen. Revealed biochemical heterogeneity suggests functional specialization of individual cells within a population. Analysis of the fluorescence quenching revealed temperature intervals in which processes of recrystallization of intracellular water associated with specific pigment-protein complexes take place...
January 2015: Biofizika
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