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Xingbo P Sun, Amy G Wilson, Georgina M Michael
Plantar fasciitis is one of the most common conditions encountered by a podiatric physician. Although most individuals respond well to traditional conservative and surgical remedies, a portion of patients will exhaust all available treatment options and will experience ongoing pain that can ultimately affect their quality of life. There has been an increase in scientific and clinical research surrounding the medical use of human placental membranes (HPMs) and many of these point-of-care allografts are now commercially available...
May 2018: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
Eric L Johnson, Elisabet K Tassis, Georgina M Michael, Susan G Whittinghill
RATIONALE: Occupational burn injuries can be detrimental and difficult to manage. The majority of complex cases are referred and managed at regional burn centers where access to specialized care is available. As an alternative to hospitalization with staged surgical procedures, placental products may be used for outpatient medical management of these common burn injuries, especially if access to a regional burn center is limited or restricted.Fresh amnion has been a treatment of choice in burns for more than 100 years...
December 2017: Medicine (Baltimore)
Jonathan Smedley, Georgina M Michael, Yeabsera G Tamire
Despite ongoing smoking cessation efforts and optimized perfusion, failed wound closure in the presence of peripheral arterial disease (PAD) and diabetes are common. A clinical effectiveness review was conducted in actively smoking diabetic patients diagnosed with PAD, treated with serial applications of a viable intact cryopreserved human placental membrane (vCPM) (Grafix, Osiris Therapeutics Inc, Columbia, MD) for recalcitrant lower extremity ulcerations (n = 6). More than half of the patients were not candidates for revascularization...
December 2016: International Journal of Lower Extremity Wounds
K Suzuki, G Michael, Y Tamire
OBJECTIVE: Advances in wound treatment have yielded successful approaches to complex wound closure through plastic surgery or other staged surgical methods. However, not all patients or wounds are candidates for such increasingly rigorous levels of interventions. The exploration of non-surgical treatment options that promote anatomical and functional tissue replacement on an outpatient basis without donor site morbidity is necessary to maximise opportunities for patient mobility and rehabilitation...
October 2016: Journal of Wound Care
Gary W Gibbons
Objective: To discuss the use of Grafix(®), a commercially available, cryopreserved placental membrane, for the treatment of chronic/stalled wounds of different etiologies. Approach: To describe the unique composition of Grafix, to provide an overview of the existing clinical evidence supporting the benefits of Grafix for wound treatment, and to share the experience of the South Shore Hospital Center for Wound Healing (Weymouth, MA) with Grafix for the treatment of nonhealing wounds. Results: Clinical evidence supports the safety and efficacy of Grafix for the treatment of chronic/stalled wounds, including those that have failed other advanced treatment modalities...
September 1, 2015: Advances in Wound Care
Dheva Setiaputra, James D Ross, Shan Lu, Derrick T Cheng, Meng-Qiu Dong, Calvin K Yip
The Spt-Ada-Gcn5 acetyltransferase (SAGA) complex is a highly conserved, 19-subunit histone acetyltransferase complex that activates transcription through acetylation and deubiquitination of nucleosomal histones in Saccharomyces cerevisiae. Because SAGA has been shown to display conformational variability, we applied gradient fixation to stabilize purified SAGA and systematically analyzed this flexibility using single-particle EM. Our two- and three-dimensional studies show that SAGA adopts three major conformations, and mutations of specific subunits affect the distribution among these...
April 17, 2015: Journal of Biological Chemistry
Lawrence A Lavery, James Fulmer, Karry Ann Shebetka, Matthew Regulski, Dean Vayser, David Fried, Howard Kashefsky, Tammy M Owings, Janaki Nadarajah
In a randomised, controlled study, we compared the efficacy of Grafix(®) , a human viable wound matrix (hVWM) (N = 50), to standard wound care (n = 47) to heal diabetic foot ulcers (DFUs). The primary endpoint was the proportion of patients with complete wound closure by 12 weeks. Secondary endpoints included the time to wound closure, adverse events and wound closure in the crossover phase. The proportion of patients who achieved complete wound closure was significantly higher in patients who received Grafix (62%) compared with controls (21%, P = 0·0001)...
October 2014: International Wound Journal
Irati R Saez de Urabain, Mark H Johnson, Tim J Smith
Fixation durations (FD) have been used widely as a measurement of information processing and attention. However, issues like data quality can seriously influence the accuracy of the fixation detection methods and, thus, affect the validity of our results (Holmqvist, Nyström, & Mulvey, 2012). This is crucial when studying special populations such as infants, where common issues with testing (e.g., high degree of movement, unreliable eye detection, low spatial precision) result in highly variable data quality and render existing FD detection approaches highly time consuming (hand-coding) or imprecise (automatic detection)...
March 2015: Behavior Research Methods
H Ulrich Göringer, Holger Stark, Cordula Böhm, Bjoern Sander, Monika M Golas
RNA editing within the mitochondria of kinetoplastid protozoa is performed by a multicomponent -macromolecular machine known as the editosome. Editosomes are high molecular mass protein assemblies that consist of about 15-25 individual polypeptides. They bind pre-edited transcripts and convert them into translation-competent mRNAs through a biochemical reaction cycle of enzyme-catalyzed steps. At steady-state conditions, several distinct complexes can be purified from mitochondrial detergent lysates. They likely represent RNA editing complexes at different assembly stages or at different functional stages of the processing reaction...
2011: Methods in Molecular Biology
Holger Stark
Here, we review the GraFix (Gradient Fixation) method to purify and stabilize macromolecular complexes for single particle cryo-electron microscopy (cryo-EM). During GraFix, macromolecules undergo a weak, intramolecular chemical cross-linking while being purified by density gradient ultracentrifugation. GraFix-stabilized particles can be used directly for negative-stain cryo-EM or, after a brief buffer-exchange step, for unstained cryo-EM. This highly reproducible method has proved to dramatically reduce problems in heterogeneity due to particle dissociation during EM grid preparation...
2010: Methods in Enzymology
Florian M Richter, Bjoern Sander, Monika M Golas, Holger Stark, Henning Urlaub
Many fundamental processes in the cell are performed by complex macromolecular assemblies that comprise a large number of proteins. Numerous macromolecular assemblies are structurally rather fragile and may suffer during purification, resulting in the partial dissociation of the complexes. These limitations can be overcome by chemical fixation of the assemblies, and recently introduced protocols such as gradient fixation during ultracentrifugation (GraFix) offer advantages for the analysis of fragile macromolecular assemblies...
August 2010: Molecular & Cellular Proteomics: MCP
Berthold Kastner, Niels Fischer, Monika Mariola Golas, Bjoern Sander, Prakash Dube, Daniel Boehringer, Klaus Hartmuth, Jochen Deckert, Florian Hauer, Elmar Wolf, Hannes Uchtenhagen, Henning Urlaub, Franz Herzog, Jan Michael Peters, Dietmar Poerschke, Reinhard Lührmann, Holger Stark
We developed a method, named GraFix, that considerably improves sample quality for structure determination by single-particle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize individual macromolecules. The method can be used to prepare samples for negative-stain, cryo-negative-stain and, particularly, unstained cryo-EM.
January 2008: Nature Methods
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