Haopeng Lin, Kunimasa Suzuki, Nancy Smith, Xi Li, Lisa Nalbach, Sonia Fuentes, Aliya F Spigelman, Xiao-Qing Dai, Austin Bautista, Mourad Ferdaoussi, Saloni Aggarwal, Andrew R Pepper, Leticia P Roma, Emmanuel Ampofo, Wen-Hong Li, Patrick E MacDonald
Pancreatic β-cells respond to metabolic stress by upregulating insulin secretion, however the underlying mechanisms remain unclear. Here we show, in β-cells from overweight humans without diabetes and mice fed a high-fat diet for 2 days, insulin exocytosis and secretion are enhanced without increased Ca2+ influx. RNA-seq of sorted β-cells suggests altered metabolic pathways early following high fat diet, where we find increased basal oxygen consumption and proton leak, but a more reduced cytosolic redox state...
January 6, 2024: Nature Communications