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https://www.readbyqxmd.com/read/29141028/developing-methods-for-assessing-abundance-and-distribution-of-european-oysters-ostrea-edulis-using-towed-video
#1
Linnea Thorngren, Thomas Dunér Holthuis, Susanne Lindegarth, Mats Lindegarth
Due to large-scale habitat losses and increasing pressures, benthic habitats in general, and perhaps oyster beds in particular, are commonly in decline and severely threatened on regional and global scales. Appropriate and cost-efficient methods for mapping and monitoring of the distribution, abundance and quality of remaining oyster populations are fundamental for sustainable management and conservation of these habitats and their associated values. Towed video has emerged as a promising method for surveying benthic communities in a both non-destructive and cost-efficient way...
2017: PloS One
https://www.readbyqxmd.com/read/29140980/prokaryotic-diversity-and-biogeochemical-characteristics-of-benthic-microbial-ecosystems-at-la-brava-a-hypersaline-lake-at-salar-de-atacama-chile
#2
Maria Eugenia Farias, Maria Cecilia Rasuk, Kimberley L Gallagher, Manuel Contreras, Daniel Kurth, Ana Beatriz Fernandez, Daniel Poiré, Fernando Novoa, Pieter T Visscher
Benthic microbial ecosystems of Laguna La Brava, Salar de Atacama, a high altitude hypersaline lake, were characterized in terms of bacterial and archaeal diversity, biogeochemistry, (including O2 and sulfide depth profiles and mineralogy), and physicochemical characteristics. La Brava is one of several lakes in the Salar de Atacama where microbial communities are growing in extreme conditions, including high salinity, high solar insolation, and high levels of metals such as lithium, arsenic, magnesium, and calcium...
2017: PloS One
https://www.readbyqxmd.com/read/29139298/sic-nanofiber-mat-a-broadband-microwave-absorber-and-the-alignment-effect
#3
Yi Hou, Laifei Cheng, Yani Zhang, Yong Yang, Chaoran Deng, Zhihong Yang, Qi Chen, Xiaoqing Du, Lianxi Zheng
Fiber alignment is a key factor that determines the physical properties of nanofiber mats. In this work, SiC nanofiber mats with or without fiber alignment are fabricated via electrospinning and the microwave electromagnetic (EM) properties of their silicone resin composites (5wt.%) are investigated in 2-18 GHz. By comparing with the composite containing smashed nanofiber, it is found that the nanofiber mats show superior dielectric loss and a minimal reflection loss (RL) of around -49 dB at 8.6 GHz and 4.3 mm thickness, associated with a broad effective absorption (<-10 dB) bandwidth (EAB) of about 7...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29139186/factors-associated-with-home-hazards-findings-from-the-malaysian-elders-longitudinal-research-study
#4
Muhammad H Romli, Maw P Tan, Lynette Mackenzie, Meryl Lovarini, Shahrul B Kamaruzzaman, Lindy Clemson
AIM: Previous studies have investigated home hazards as a risk factor for falls without considering factors associated with the presence of home hazards. The present study aimed to determine patterns of home hazards among urban community-dwelling older Malaysians, and to identify factors contributing to home hazards. METHODS: Cross-sectional data from the initial wave of the Malaysian Elders Longitudinal Research study were used. Basic demographics were obtained from the Global Questionnaire...
November 15, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/29138464/bone-regeneration-is-associated-with-the-concentration-of-tumour-necrosis-factor-%C3%AE-induced-by-sericin-released-from-a-silk-mat
#5
You-Young Jo, HaeYong Kweon, Dae-Won Kim, Kyunghwa Baek, Min-Keun Kim, Seong-Gon Kim, Weon-Sik Chae, Je-Yong Choi, Horatiu Rotaru
To understand the osteogenic effect of the middle layer of the silk cocoon, sericin was examined for its cellular effects associated with tumor necrosis factor-α (TNF-α) signaling in this study. The fragmented sericin proteins in the silk mat were evaluated for the TNF-α expression level in murine macrophages. The concentration of protein released from silk mats was higher in the outermost and the innermost layers than in the middle layers, and the protein released from the silk mat was identified as sericin...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29135427/effect-of-bathroom-aids-and-age-on-balance-control-during-bathing-transfers
#6
Emily C King, Alison C Novak
Bathroom assistive devices are used to improve safety during bathing transfers, but biomechanical evidence to support clinical recommendations is lacking. This study evaluated the effectiveness of common bathroom aids in promoting balance control during bathing transfers. Twenty-six healthy adults (12 young, 14 older) stepped into and out of a slippery bathtub while using a vertical grab bar on the side wall, a horizontal grab bar on the back wall, a bath mat, a side wall touch, or no assistance. Balance control was characterized using center of pressure measures and showed greater instability for older adults...
November 2017: American Journal of Occupational Therapy: Official Publication of the American Occupational Therapy Association
https://www.readbyqxmd.com/read/29135301/microbialite-biosignature-analysis-by-mesoscale-x-ray-fluorescence-%C3%AE-xrf-mapping
#7
Michael M Tice, Kimbra Quezergue, Michael C Pope
As part of its biosignature detection package, the Mars 2020 rover will carry PIXL, the Planetary Instrument for X-ray Lithochemistry, a spatially resolved X-ray fluorescence (μXRF) spectrometer. Understanding the types of biosignatures detectable by μXRF and the rock types μXRF is most effective at analyzing is therefore an important goal in preparation for in situ Mars 2020 science and sample selection. We tested mesoscale chemical mapping for biosignature interpretation in microbialites. In particular, we used μXRF to identify spatial distributions and associations between various elements ("fluorescence microfacies") to infer the physical, biological, and chemical processes that produced the observed compositional distributions...
November 2017: Astrobiology
https://www.readbyqxmd.com/read/29134948/effect-of-scaffold-morphology-and-cell-co-culture-on-tenogenic-differentiation-of-hadmsc-on-centrifugal-melt-electrospun-poly-l%C3%A2-lactic-acid-fibrous-meshes
#8
Shaohua Wu, Hao Peng, Xiuhong Li, Philipp N Streubel, Yong Liu, Bin Duan
Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29134783/electrospun-titania-fiber-mats-spin-coated-with-thin-polymer-films-as-nanofibrous-scaffolds-for-enhanced-cell-proliferation
#9
Alfin Kurniawan, Chintya Effendi, Meng-Jiy Wang
The incorporation of inorganic materials into electrospun nanofibers has recently gained considerable attention for the development of ECM-like scaffolds with improved mechanical properties and enhanced biological functions for tissue engineering applications. In this study, polymer-inorganic composite fibers consisting of poly(2-ethyl-2-oxazoline) (PEOXA) and tetrabutyl titanate (TBT) as the titanium precursor were successfully fabricated through a combined sol-gel/electrospinning approach. PEOXA/Ti(OR)n composite fibers were obtained with varying amounts of polymer and titanium precursors...
November 14, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29133847/female-mice-lacking-pald1-exhibit-endothelial-cell-apoptosis-and-emphysema
#10
Isabel Egaña, Hiroshi Kaito, Anja Nitzsche, Lore Becker, Carolina Ballester-Lopez, Colin Niaudet, Milena Petkova, Wei Liu, Michael Vanlandewijck, Alexandra Vernaleken, Thomas Klopstock, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabe de Angelis, Helge Rask-Andersen, Henrik J Johansson, Janne Lehtiö, Liqun He, Ali Ö Yildirim, Mats Hellström
Paladin (Pald1, mKIAA1274 or x99384) was identified in screens for vascular-specific genes and is a putative phosphatase. Paladin has also been proposed to be involved in various biological processes such as insulin signaling, innate immunity and neural crest migration. To determine the role of paladin we have now characterized the Pald1 knock-out mouse in a broad array of behavioral, physiological and biochemical tests. Here, we show that female, but not male, Pald1 heterozygous and homozygous knock-out mice display an emphysema-like histology with increased alveolar air spaces and impaired lung function with an obstructive phenotype...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29133280/mat2a-promotes-porcine-adipogenesis-by-mediating-h3k27me3-at-wnt10b-locus-and-repressing-wnt-%C3%AE-catenin-signaling
#11
Cunzhen Zhao, Haigang Wu, Naren Qimuge, Weijun Pang, Xiao Li, Guiyan Chu, Gongshe Yang
Methionine adenosyltransferase (MAT) is a critical biological enzyme and that can catalyze L-met and ATP to form S-adenosylmethionine (SAM), which is acted as a biological methyl donor in transmethylation reactions involving histone methylation. However, the regulatory effect of methionine adenosyltransferase2A (MAT2A) and its associated methyltransferase activity on adipogenesis is still unclear. In this study, we investigate the effect of MAT2A on adipogenesis and its potential mechanism on histone methylation during porcine preadipocyte differentiation...
November 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29133102/development-of-bioactive-cellulose-nanocrystals-derived-from-dominant-cellulose-polymorphs-i-and-ii-from-capsosiphon-fulvescens-for-biomedical-applications
#12
Sung Won Ko, Juan Paolo E Soriano, Afeesh Rajan Unnithan, Ji Yeon Lee, Chan Hee Park, Cheol Sang Kim
Cellulose I and II polymorphs were isolated from Capsosiphon fulvescens (CF) using the conventional method of extraction and direct mercerization of raw sample, respectively. The morphological and structural differences between the isolated polymorphs were studied by FTIR, FESEM and XRD. Direct mercerization of raw CF yielded the transformation of highly crystalline cellulose I (81.3%) to II (63.7%) as observed in the shifting of XRD patterns. The derived cellulose I and II were hydrolyzed (60% w/w H2SO4, 55°C, 1hr, 10mL/g) to obtain the spindle-shaped cellulose nanocrystals...
November 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29132978/intensive-care-in-severe-malaria-report-from-the-task-force-on-tropical-diseases-by-the-world-federation-of-societies-of-intensive-and-critical-care-medicine
#13
REVIEW
Dilip R Karnad, Mohd Basri Mat Nor, Guy A Richards, Tim Baker, Pravin Amin
Severe malaria is common in tropical countries in Africa, Asia, Oceania and South and Central America. It may also occur in travelers returning from endemic areas. Plasmodium falciparum accounts for most cases, although P vivax is increasingly found to cause severe malaria in Asia. Cerebral malaria is common in children in Africa, manifests as coma and seizures, and has a high morbidity and mortality. In other regions, adults may also develop cerebral malaria but neurological sequelae in survivors are rare...
November 8, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/29131400/evaluation-of-3-serological-tests-for-early-detection-of-leptospira-specific-antibodies-in-experimentally-infected-dogs
#14
J Lizer, S Velineni, A Weber, M Krecic, P Meeus
BACKGROUND: Leptospirosis in dogs is a disease of global importance. Early detection and appropriate therapeutic intervention are necessary to resolve infection and prevent zoonotic transmission. However, its diagnosis is hindered by nonspecific clinical signs and lack of rapid diagnostic tests of early infection. Recently, 2 rapid point-of-care tests (WITNESS Lepto [WITNESS Lepto, Zoetis LLC, Kalamazoo, MI, USA] and SNAP Lepto [SNAP Lepto, IDEXX Laboratories, Westbrook, ME, USA]) for detection of Leptospira-specific antibodies in canine sera were developed...
November 13, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29130200/padlock-probes-to-detect-single-nucleotide-polymorphisms
#15
Tomasz Krzywkowski, Mats Nilsson
Rapid development of high-throughput DNA analyzation methods has enabled global characterization of genetic landscapes and aberrations in study subjects in a time and cost effective fashion. In most methods, however, spatial tissue context is lost since sample preparation requires isolation of nucleic acids out of their native environment. We hereby present the most recent protocol for multiplexed, in situ detection of mRNAs and single nucleotide polymorphisms using padlock probes and rolling circle amplification...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29129734/ciprofloxacin-loaded-sodium-alginate-poly-lactic-co-glycolic-acid-electrospun-fibrous-mats-for-wound-healing
#16
Xiaoli Liu, Line Hagner Nielsen, Sylvia Natalie Klodzinska, Hanne Mørck Nielsen, Haiyan Qu, Lars Porskjær Christensen, Jukka Rantanen, Mingshi Yang
Wound dressings should ideally be able to maintain high humidity, remove excess wound exudate, permit thermal insulation, provide certain mechanical strength, and in some cases deliver antibiotics to prevent infections. Until now, none of the existing wound dressing products can meet all these requirements. To design a wound dressing with as many of the aforementioned features as possible, in this study, we attempted to prepare ciprofloxacin (CIP), an antibiotic, loaded electrospun hydrophobic poly (lactic-co-glycolic acid) (PLGA) fibrous mats modified with hydrophilic sodium alginate (ALG) microparticles...
November 9, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29129538/pneumonia-in-the-tropics-report-from-the-task-force-on-tropical-diseases-by-the-world-federation-of-societies-of-intensive-and-critical-care-medicine
#17
REVIEW
Mohd Basri Mat Nor, Guy A Richards, Steve McGloughlin, Pravin R Amin
The aetiology of community acquired pneumonia varies according to the region in which it is acquired. This review discusses those causes of CAP that occur in the tropics and might not be readily recognizable when transplanted to other sites. Various forms of pneumonia including the viral causes such as influenza (seasonal and avian varieties), the coronaviruses and the Hantavirus as well as bacterial causes, specifically the pneumonic form of Yersinia pestis and melioidosis are discussed.
November 3, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/29128968/involvement-of-cyclodipeptides-in-the-competition-of-bacterial-communities-in-the-oligotrophic-churince-aquatic-system-of-cuatro-ci%C3%A3-negas-basin-dominated-by-gammaproteobacteria
#18
Enrique Martínez-Carranza, Gabriel Y Ponce-Soto, Alma L Díaz-Pérez, Erasmo Cadenas, Valeria Souza, Jesús Campos-García
The Cuatro Ciénegas Basin (CCB) within the Chihuahuan Desert in México is an extremely oligotrophic oasis with negligible phosphorous levels, described as a hot spot of biodiversity, not only in stromatolites and microbial mats, but also in living forms in general. The microorganisms possess the capability to produce a wide variety of virulence factors, antibiotics, and quorum-sensing (QS) crosstalk signals such as non-ribosomal cyclodipeptides (CDPs) which enables them to colonize diverse ecological niches...
November 11, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29128910/allogeneic-ovarian-transplantation-using-immunomodulator-preimplantation-factor-pif-as-monotherapy-restored-ovarian-function-in-olive-baboon
#19
Michael Feichtinger, Eytan R Barnea, Atunga Nyachieo, Mats Brännström, S Samuel Kim
PURPOSE: Allogeneic ovarian transplantation may be an alternative in the future to oocyte donation in women with premature ovarian failure. The objectives of this study were to (a) evaluate allotransplantation feasibility for restoration of ovarian function and (b) assess efficacy of synthetic preimplantation factor (PIF) monotherapy as sole immune-acceptance regimen. METHODS: This is an experimental animal study using non-human primates (Papio anubis). Allogeneic orthotopic ovarian tissue transplantation was performed in two female olive baboons...
November 11, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29128754/development-and-characterization-of-a-human-monoclonal-antibody-targeting-the-n-terminal-region-of-hepatitis-c-virus-envelope-glycoprotein-e1
#20
Ahmed Atef Mesalam, Isabelle Desombere, Ali Farhoudi, Freya Van Houtte, Lieven Verhoye, Jonathan Ball, Jean Dubuisson, Steven K H Foung, Arvind H Patel, Mats A A Persson, Geert Leroux-Roels, Philip Meuleman
Monoclonal antibodies (mAbs) targeting the hepatitis C virus (HCV) envelope have been raised mainly against envelope protein 2 (E2), while the antigenic epitopes of envelope protein 1 (E1) are not fully identified. Here we describe the detailed characterization of a human mAb, designated A6, generated from an HCV genotype 1b infected patient. ELISA results showed reactivity of mAb A6 to full-length HCV E1E2 of genotypes 1a, 1b and 2a. Epitope mapping identified a region spanning amino acids 230-239 within the N-terminal region of E1 as critical for binding...
November 9, 2017: Virology
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