keyword
MENU ▼
Read by QxMD icon Read
search

Alpha defensin

keyword
https://www.readbyqxmd.com/read/28077642/studies-on-the-interaction-of-tumor-derived-hd5-alpha-defensins-with-adenoviruses-and-implications-for-oncolytic-adenovirus-therapy
#1
Charles Vragniau, Jens-Martin Hübner, Peter Beidler, Sucheol Gil, Kamola Saydaminova, Zhuo-Zhuang Lu, Roma Yumul, Hongjie Wang, Maximilian Richter, Pavel Sova, Charles Drescher, Pascal Fender, André Lieber
: Defensins are small anti-microbial peptides capable of neutralizing human adenovirus (HAdV) in vitro by binding capsid proteins and blocking endosomal escape of virus. In humans, the alpha defensin HD5 is produced by specialized epithelial cells of the gastro-intestinal and genito-urinary tracts. Here we demonstrate that HD5 is also expressed as an active, secreted peptide by epithelial ovarian and lung cancer cells in situ, in patient biopsies. This finding triggered us to study the role of HD5 in infection and spread of replication-competent, oncolytic HAdV type 3 virus...
January 11, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28038935/defense-peptide-repertoire-of-stellaria-media-predicted-by-high-throughput-next-generation-sequencing
#2
Anna A Slavokhotova, Andrey A Shelenkov, Tatyana V Korostyleva, Eugene A Rogozhin, Nataliya V Melnikova, Anna V Kudryavtseva, Tatyana I Odintsova
Being perfectly adapted to diverse environments, chickweed (Stellaria media (L.) Vill), a ubiquitous garden weed, grows widely in Europe and North America. As opposed to the model plants, many weeds, and S. media in particular, have been poorly studied, although they are likely to contain promising components of immunity and novel resistance genes. In this study, for the first time RNA-seq analysis of healthy and infected with Fusarium oxysporum chickweed seedlings, as well as de novo transcriptome assembly and annotation, are presented...
December 27, 2016: Biochimie
https://www.readbyqxmd.com/read/27959380/sequencing-and-bioinformatics-analysis-of-the-differentially-expressed-genes-in-herniated-discs-with-or-without-calcification
#3
Jia Shao, Miao Yu, Liang Jiang, Fengliang Wu, Xiaoguang Liu
The purpose of this study was to detect the differentially expressed genes between ossified herniated discs and herniated discs without ossification. In addition, we sought to identify a few candidate genes and pathways by using bioinformatics analysis. We analyzed 6 samples each of ossified herniated discs (experimental group) and herniated discs without ossification (control group). Purified mRNA and cDNA extracted from the samples were subjected to sequencing. The NOISeq method was used to statistically identify the differentially expressed genes (DEGs) between the 2 groups...
January 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/27895587/vitamin-d-signaling-through-induction-of-paneth-cell-defensins-maintains-gut-microbiota-and-improves-metabolic-disorders-and-hepatic-steatosis-in-animal-models
#4
Danmei Su, Yuanyang Nie, Airu Zhu, Zishuo Chen, Pengfei Wu, Li Zhang, Mei Luo, Qun Sun, Linbi Cai, Yuchen Lai, Zhixiong Xiao, Zhongping Duan, Sujun Zheng, Guihui Wu, Richard Hu, Hidekazu Tsukamoto, Aurelia Lugea, Zhenqui Liu, Stephen J Pandol, Yuan-Ping Han
Metabolic syndrome (MetS), characterized as obesity, insulin resistance, and non-alcoholic fatty liver diseases (NAFLD), is associated with vitamin D insufficiency/deficiency in epidemiological studies, while the underlying mechanism is poorly addressed. On the other hand, disorder of gut microbiota, namely dysbiosis, is known to cause MetS and NAFLD. It is also known that systemic inflammation blocks insulin signaling pathways, leading to insulin resistance and glucose intolerance, which are the driving force for hepatic steatosis...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27863510/establishing-a-reference-array-for-the-cs-%C3%AE-%C3%AE-superfamily-of-defensive-peptides
#5
D Ellen K Tarr
BACKGROUND: "Invertebrate defensins" belong to the cysteine-stabilized alpha-beta (CS-αβ), also known as the scorpion toxin-like, superfamily. Some other peptides belonging to this superfamily of defensive peptides are indistinguishable from "defensins," but have been assigned other names, making it unclear what, if any, criteria must be met to qualify as an "invertebrate defensin." In addition, there are other groups of defensins in invertebrates and vertebrates that are considered to be evolutionarily unrelated to those in the CS-αβ superfamily...
November 18, 2016: BMC Research Notes
https://www.readbyqxmd.com/read/27714447/alpha-defensin-novel-synovial-fluid-biomarker-for-the-diagnosis-of-periprosthetic-joint-infection
#6
Jakrapun Pupaibool, Eric J Fulnecky, Robert L Swords, William W Sistrunk, Alastair D Haddow
PURPOSE: Periprosthetic joint infection (PJI) continues to be a significant complication after total joint arthroplasty. Diagnosis of PJI can be challenging and elusive in the absence of a gold standard. This purpose of this article is to review the current evidence on the utility of serum and synovial fluid biomarkers to help aid in the diagnosis of PJI with focusing on synovial fluid alpha-defensin. METHODS: Articles and data for this review were identified by searches of PubMed and Ovid MEDLINE up to June 1, 2016...
October 7, 2016: International Orthopaedics
https://www.readbyqxmd.com/read/27538253/-diagnosis-of-periprosthetic-infection-what-s-obligation-what-s-optional
#7
K-D Heller
BACKGROUND: Periprothetic infection after primary or revision arthroplasty is of increasing importance. The incidence of infection in primary arthroplasty is approximately 1 %. Revision arthroplasty has higher infection rates, which increase with the number of revision surgeries. Aim of this Paper: An overview of the current diagnosis of periprosthetic infection. METHODS: This selective analysis is based on a lecture by the author at the DKOU 2015. RESULTS: Periprosthetic infection is taken as having been demonstrated when either two periprosthetic cultures contain the same microorganism, or if there is a fistula communicating with a joint...
August 2016: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/27525335/alpha-defensin-5-expression-is-regulated-by-micrornas-in-the-caco-2-intestinal-epithelial-cell-line
#8
Donald R B Miles, Jun Shen, Alice Y Chuang, Fenshi Dong, Feng Wu, John Kwon
BACKGROUND: In inflammatory bowel disease (IBD), an inappropriate immune response leads to chronic mucosal inflammation. This response may be partly due to dysregulation of defensins, which are endogenously produced antimicrobial peptides. This study determined whether microRNAs (miRNAs) regulate α-defensin 5 (DEFA5), which could further implicate both in IBD pathogenesis. METHODS: Induction of DEFA5 mRNA and protein expression was determined in Caco-2 cells. An in silico analysis identified putative miRNA binding sites of DEFA5...
April 2016: Journal of Inflammatory Bowel Diseases & Disorders
https://www.readbyqxmd.com/read/27444031/corr-insights-%C3%A2-how-reliable-is-the-alpha-defensin-immunoassay-test-for-diagnosing-periprosthetic-joint-infection-a-prospective-study
#9
Thomas W Bauer
No abstract text is available yet for this article.
February 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/27430968/increased-alpha-defensin-expression-is-associated-with-risk-of-coronary-heart-disease-a-feasible-predictive-inflammatory-biomarker-of-coronary-heart-disease-in-hyperlipidemia-patients
#10
Yaowapa Maneerat, Kriengchai Prasongsukarn, Surachet Benjathummarak, Wilanee Dechkhajorn, Urai Chaisri
BACKGROUND: Atherosclerosis is a multifactorial disorder of the heart vessels that develops over decades, coupling inflammatory mechanisms and elevated total cholesterol levels under the influence of genetic and environmental factors. Without effective intervention, atherosclerosis consequently causes coronary heart disease (CHD), which leads to increased risk of sudden death. Polymorphonuclear neutrophils play a pivotal role in inflammation and atherogenesis. Human neutrophil peptides (HNPs) or alpha (α)-defensins are cysteine-rich cation polypeptides that are produced and released from activated polymorphonuclear neutrophil granules during septic inflammation and acute coronary vascular disorders...
July 18, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27402133/immune-response-in-human-chromoblastomycosis-and-eumycetoma-focusing-on-human-interleukin-17a-interferon-gamma-tumour-necrosis-factor-alpha-interleukin-1-beta-and-human-beta-defensin-2
#11
Charussri Leeyaphan, Carren Hau, Shintaro Takeoka, Yayoi Tada, Sumanas Bunyaratavej, Penvadee Pattanaprichakul, Panitta Sitthinamsuwan, Angkana Chaiprasert, Yuko Sasajima, Koichi Makimura, Shinichi Watanabe
Knowledge regarding host immune response to chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin-embedded tissue samples from patients diagnosed with chromoblastomycosis or eumycetoma from histological findings and tissue culture. DNA extraction and polymerase chain reaction (PCR) from tissues were done to evaluate human interleukin-17A (IL-17A), interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and human beta-defensin-2 (HBD-2) expressions...
July 12, 2016: Mycoses
https://www.readbyqxmd.com/read/27343056/how-reliable-is-the-alpha-defensin-immunoassay-test-for-diagnosing-periprosthetic-joint-infection-a-prospective-study
#12
Tommaso Bonanzinga, Akos Zahar, Michael Dütsch, Christian Lausmann, Daniel Kendoff, Thorsten Gehrke
BACKGROUND: A key issue in the treatment of periprosthetic joint infection (PJI) is the correct diagnosis. The main problem is lack of diagnostic tools able to diagnose a PJI with high accuracy. Alpha-defensin has been proposed as a possible solution, but in the current literature, there is a lack of independent validation. QUESTIONS/PURPOSES: We performed a prospective study to determine (1) what is the sensitivity, specificity, and positive and the negative predictive values of the alpha-defensin immunoassay test in diagnosing PJI; and (2) which clinical features may be responsible for false-positive and false-negative results? METHODS: Preoperative aspiration was performed in all patients presenting with a painful hip/knee arthroplasty, including both primary and revision implants...
February 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/27329580/intraoperative-diagnosis-of-periprosthetic-joint-infection-using-a-novel-alpha-defensin-lateral-flow-assay
#13
Maximilian F Kasparek, Michael Kasparek, Friedrich Boettner, Martin Faschingbauer, Julia Hahne, Martin Dominkus
BACKGROUND: The present study investigates the novel Synovasure periprosthetic joint infection (PJI) lateral flow test device for detection of alpha-defensin and attempts to determine its diagnostic accuracy for the intraoperative diagnosis of PJI and compares it to frozen section. METHODS: Forty consecutive patients, who underwent revision surgery, between September 2014 and September 2015 were included. The patients underwent 29 revision total knee arthroplasties and 11 revision total hip arthroplasties...
May 27, 2016: Journal of Arthroplasty
https://www.readbyqxmd.com/read/27307370/a-compelling-new-diagnostic-option-for-identification-of-orthopaedic-infections-commentary-on-an-article-by-m-c-wyatt-bsc-hons-mbchb-mrcs-ed-fracs-tr-orth-et-al-the-alpha-defensin-immunoassay-and-leukocyte-esterase-colorimetric-strip-test-for-the-diagnosis
#14
https://www.readbyqxmd.com/read/27307359/the-alpha-defensin-immunoassay-and-leukocyte-esterase-colorimetric-strip-test-for-the-diagnosis-of-periprosthetic-infection-a-systematic-review-and-meta-analysis
#15
M C Wyatt, A D Beswick, S K Kunutsor, M J Wilson, M R Whitehouse, A W Blom
BACKGROUND: Synovial biomarkers have recently been adopted as diagnostic tools for periprosthetic joint infection (PJI), but their utility is uncertain. The purpose of this systematic review and meta-analysis was to synthesize the evidence on the accuracy of the alpha-defensin immunoassay and leukocyte esterase colorimetric strip test for the diagnosis of PJI compared with the Musculoskeletal Infection Society diagnostic criteria. METHODS: We performed a systematic review to identify diagnostic technique studies evaluating the accuracy of alpha-defensin or leukocyte esterase in the diagnosis of PJI...
June 15, 2016: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/27298819/a-novel-way-of-treating-multidrug-resistant-enterococci
#16
Hem Desai, Ryan Wong, Ahmed Khurshid Pasha
CONTEXT: Daptomycin is the only antibiotic available with in vitro bactericidal activity against vancomycin-resistant enterococci (VRE). Its increased use has resulted in cases of decreased daptomycin efficacy. Recent in vitro studies have shown effective use of beta (β)-lactam and daptomycin antibiotics, as a combination therapy, in the treatment of VRE. We describe a case of effective treatment in a patient with VRE infection using dual ampicillin and daptomycin therapy that shows bench-to-bedside application of the abovementioned finding...
May 2016: North American Journal of Medical Sciences
https://www.readbyqxmd.com/read/27241375/diagnosis-of-periprosthetic-joint-infection-following-hip-and-knee-arthroplasty
#17
REVIEW
Javad Parvizi, Safa Cyrus Fassihi, Mohammad A Enayatollahi
The diagnosis of periprosthetic joint infection (PJI) following total hip arthroplasty and total knee arthroplasty has been one of the major challenges in orthopedic surgery. As there is no single absolute test for diagnosis of PJI, diagnostic criteria for PJI have been proposed that include using several diagnostic modalities. Focused history, physical examination, plain radiographs, and initial serologic tests should be followed by joint aspiration and synovial analysis. Newer diagnostic techniques, such as alpha-defensin and interleukin-6, hold great promise in the future diagnosis of equivocal infections...
July 2016: Orthopedic Clinics of North America
https://www.readbyqxmd.com/read/27088930/local-antimicrobial-protease-and-cytokine-defense-systems-in-psoriatic-skin
#18
Elga Sidhom, Mara Pilmane, Janis Kisis
BACKGROUND: Psoriasis vulgaris is an inflammatory skin condition characterized by dramatic biochemical and immunological changes. AIMS: The aim of the study was to evaluate antimicrobial response, tissue degeneration reactions and distribution of inflammatory cytokines in untreated psoriatic skin as well as the correlations between these factors and influence on the course of the disease. METHODS: We evaluated skin samples obtained from routine punch biopsies in 40 patients with psoriasis vulgaris...
May 2016: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/27044258/cellular-localization-of-guanylin-and-uroguanylin-mrnas-in-human-and-rat-duodenal-and-colonic-mucosa
#19
Øystein Brenna, Marianne W Furnes, Bjørn Munkvold, Mark Kidd, Arne K Sandvik, Björn I Gustafsson
Guanylin (GUCA2A/Guca2a/GN) and uroguanylin (GUCA2B/Guca2b/UGN) are expressed in the gastrointestinal tract and have been implicated in ion and fluid homeostasis, satiety, abdominal pain, growth and intestinal barrier integrity. Their cellular sources are debated and include goblet cells, entero-/colonocytes, enteroendocrine (EE) cells and tuft cells. We therefore investigated the cellular sources of GN and UGN mRNAs in human and rat duodenal and colonic epithelium with in situ hybridization (ISH) to determine co-expression with Chromogranin A (CHGA/Chga/CgA; enterochromaffin [EC] cells), defensin alpha 6 (DEFA6/Defa6; Paneth cells), mucin 2 (MUC2/Muc2; goblet cells) and selected tuft cell markers...
August 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27044108/neutrophil-derived-alpha-defensins-control-inflammation-by-inhibiting-macrophage-mrna-translation
#20
Matthew Brook, Gareth H Tomlinson, Katherine Miles, Richard W P Smith, Adriano G Rossi, Pieter S Hiemstra, Emily F A van 't Wout, Jonathan L E Dean, Nicola K Gray, Wuyuan Lu, Mohini Gray
Neutrophils are the first and most numerous cells to arrive at the site of an inflammatory insult and are among the first to die. We previously reported that alpha defensins, released from apoptotic human neutrophils, augmented the antimicrobial capacity of macrophages while also inhibiting the biosynthesis of proinflammatory cytokines. In vivo, alpha defensin administration protected mice from inflammation, induced by thioglychollate-induced peritonitis or following infection withSalmonella entericaserovar Typhimurium...
April 19, 2016: Proceedings of the National Academy of Sciences of the United States of America
keyword
keyword
48259
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"