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Detrusor muscle

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https://www.readbyqxmd.com/read/27905300/intravital-imaging-of-mouse-urothelium-reveals-activation-of-extracellular-signal-regulated-kinase-by-stretch-induced-intravesical-release-of-atp
#1
Takeshi Sano, Takashi Kobayashi, Hiromitsu Negoro, Atsushi Sengiku, Takuya Hiratsuka, Yuji Kamioka, Louis S Liou, Osamu Ogawa, Michiyuki Matsuda
To better understand the roles played by signaling molecules in the bladder, we established a protocol of intravital imaging of the bladder of mice expressing a Förster/fluorescence resonance energy transfer (FRET) biosensor for extracellular signal-regulated kinase (ERK), which plays critical roles not only in cell growth but also stress responses. With an upright two-photon excitation microscope and a vacuum-stabilized imaging window, cellular ERK activity was visualized in the whole bladder wall, from adventitia to urothelium...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27882470/comparison-of-detrusor-muscle-sampling-rate-in-monopolar-and-bipolar-transurethral-resection-of-bladder-tumor-a-randomized-trial
#2
Jeremy Yuen-Chun Teoh, Eddie Shu-Yin Chan, Siu-Ying Yip, Ho-Man Tam, Peter Ka-Fung Chiu, Chi-Hang Yee, Hon-Ming Wong, Chi-Kwok Chan, Simon See-Ming Hou, Chi-Fai Ng
PURPOSE: Our aim was to investigate the detrusor muscle sampling rate after monopolar versus bipolar transurethral resection of bladder tumor (TURBT). METHODS: This was a single-center, prospective, randomized, phase III trial on monopolar versus bipolar TURBT. Baseline patient characteristics, disease characteristics and perioperative outcomes were compared, with the primary outcome being the detrusor muscle sampling rate in the TURBT specimen. Multivariate logistic regression analyses on detrusor muscle sampling were performed...
November 23, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27844406/partial-bladder-outlet-obstruction-is-associated-with-decreased-expression-and-function-of-the-small-conductance-ca-2-activated-k-channel-in-guinea-pig-detrusor-smooth-muscle
#3
Ning Li, Xiaoning He, Zizheng Li, Yili Liu, Ping Wang
PURPOSE: Partial bladder outlet obstruction (PBOO) usually induces overactive bladder (OAB) associated with detrusor overactivity (DO) which is related to the increased contractility of detrusor smooth muscle (DSM). The pharmacological activation of small-conductance Ca(2+)-activated K(+) (SK) channels dramatically attenuates DSM contractility. However, the role of SK channels in the PBOO DSM is less clear. Here, we tested the hypothesis that PBOO is associated with decreased expression and function of SK channels in DSM and that the activation of SK channels is a potential target to regulate DO...
November 14, 2016: International Urology and Nephrology
https://www.readbyqxmd.com/read/27798943/effects-of-distigmine-on-electrical-field-stimulation-induced-contraction-of-mouse-urinary-bladder-smooth-muscles
#4
Keisuke Obara, Yurina Kobayashi, Daisuke Chino, Yoshio Tanaka
Distigmine bromide (distigmine), a reversible carbamate cholinesterase inhibitor, is used in Japan for treating detrusor underactivity, myasthenia gravis, and glaucoma. Although there is clinical evidence about the effectiveness of distigmine in the treatment of detrusor underactivity, mechanisms by which distigmine restores impaired urinary bladder smooth muscle (UBSM) contractility have not been fully investigated. The aims of this study were to investigate the potentiating effects of distigmine on UBSM contractions in response to parasympathetic nerve stimulation induced by electrical field stimulation (EFS) in mice...
November 1, 2016: Pharmacology
https://www.readbyqxmd.com/read/27794199/long-term-treatment-with-the-beta-3-adrenoceptor-agonist-mirabegron-ameliorates-detrusor-overactivity-and-restores-cyclic-adenosine-monophosphate-camp-levels-in-obese-mice
#5
Fabiano B Calmasini, Mariana G de Oliveira, Eduardo C Alexandre, Fábio H da Silva, Carmem P V da Silva, Tuany Z Candido, Edson Antunes, Fabíola Z Mónica
AIMS: To evaluate the effects of the beta-3 adrenoceptor agonist, mirabegron in a mouse model of detrusor overactivity induced by obesity. METHODS: C57BL/6 male mice were fed with standard chow or high-fat diet for 12 weeks. Lean and obese mice were treated orally with mirabegron (10 mg/kg/day) from the last 2 weeks of diet. Cystometric evaluations, functional assays, protein expression for phosphodiesterase type 4 (PDE4), and cyclic adenosine monophosphate (cAMP) measurement were carried out...
October 29, 2016: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/27777947/expansion-of-submucosal-bladder-wall-tissue-in-vitro-and-in-vivo
#6
Gisela Reinfeldt Engberg, Clara Ibel Chamorro, Agneta Nordenskjöld, Magdalena Fossum
In order to develop autologous tissue engineering of the whole wall in the urinary excretory system, we studied the regenerative capacity of the muscular bladder wall. Smooth muscle cell expansion on minced detrusor muscle in vitro and in vivo with or without urothelial tissue was studied. Porcine minced detrusor muscle and urothelium were cultured in vitro under standard culture conditions for evaluation of the explant technique and in collagen for tissue sectioning and histology. Autografts of minced detrusor muscle with or without minced urothelium were expanded on 3D cylinder moulds by grafting into the subcutaneous fat of the pig abdominal wall...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27761601/kv7-channels-in-the-human-detrusor-channel-modulator-effects-and-gene-and-protein-expression
#7
Riccardo Bientinesi, Cesare Mancuso, Maria Martire, Pier Francesco Bassi, Emilio Sacco, Diego Currò
Voltage-gated type 7 K(+) (KV7 or KCNQ) channels regulate the contractility of various smooth muscles. With this study, we aimed to assess the role of KV7 channels in the regulation of human detrusor contractility, as well as the gene and protein expression of KV7 channels in this tissue. For these purposes, the isolated organ technique, RT-qPCR, and Western blot were used, respectively. XE-991, a selective KV7 channel blocker, concentration-dependently contracted the human detrusor; mean EC50 and Emax of XE-991-induced concentration-response curve were 14...
October 19, 2016: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/27738973/octodon-degus-a-new-model-to-study-the-agonist-and-plexus-induced-response-in-the-urinary-bladder
#8
Francisco Eduardo Martin-Cano, Mercedes Caso-Agundez, Cristina Camello-Almaraz, Francisco Juan Santos, María Teresa Espin, Juan Antonio Madrid, Adolfo Diez-Perez, Pedro Javier Camello, Maria Jose Pozo
Urinary bladder function consists in the storage and controlled voiding of urine. Translational studies require animal models that match human characteristics, such as Octodon degus, a diurnal rodent. This study aims to characterize the contractility of the detrusor muscle and the morphology and code of the vesical plexus from O. degus. Body temperature was measured by an intra-abdominal sensor, the contractility of detrusor strips was evaluated by isometric tension recording, and the vesical plexus was studied by electrical field stimulation (EFS) and immunofluorescence...
October 13, 2016: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/27709382/effect-of-doxazosin-on-autonomic-nervous-control-and-urodynamics-of-rat-urinary-bladder-during-modeled-infravesical-obstruction
#9
V I Kirpatovskii, I S Mudraya, S V Revenko, A Yu Bablumyan, N K Adamyan, V P Ivanov
The therapeutic effect of doxazosin (40 μg/kg/day over one month) on urinary bladder was examined in female rats with modeled chronic infravesical obstruction (IVO) produced by graduated mechanical constriction of the proximal urethral segment. In one month, IVO induced a pronounced vesical hypertrophy both in treated and untreated rats that manifested in increased bladder weight and capacity, the latter increment being pronouncedly greater in treated rats. In untreated IVO rats, infusion cystometry revealed elevated basal intravesical pressure of void bladder P0, markedly increased maximal (premicturitional) pressure Pmax, and increased amplitude of spontaneous oscillations of intravesical pressure ΔPdet in filled bladder...
September 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27695622/mirabegron-in-overactive-bladder-patients-efficacy-review-and-update-on-drug-safety
#10
Katherine Warren, Helena Burden, Paul Abrams
Overactive bladder is a common condition, which significantly affects people's quality of life. The use of anticholinergic medication has been the mainstay of managing overactive bladder when conservative measures are not enough. Many patients stop anticholinergic medication because of the side effects and more recently the concerns about the effect of an anticholinergic burden and the development of dementia have been studied. Activation of β3 adrenoceptors has been shown to relax the detrusor muscle and subsequently lead to the development of the first β3 adrenoceptor agonist...
October 2016: Therapeutic Advances in Drug Safety
https://www.readbyqxmd.com/read/27676715/bladder-rupture-secondary-to-emphysematous-bladder-a-diabetic-patient-report
#11
Astrid Balança
OBJECTIVE: We report an emphysematous cystitis in a diabetic patient complicated by peritonitis. OBSERVATIONS: A 66-year-old male presented to the emergency with septic shock. Patient's history: poorly controlled diabetes and prostate adenoma. Clinical examination revealed a surgical abdomen and signs of shock. Blood sugar was 6g/L. CRP 274mg/L. The abdomino-pelvic CT scan showed a perforated bladder with emphysema of the bladder wall and endoluminal air bubbles...
September 2016: Annals of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/27664012/prostaglandin-d2-effects-and-dp1-dp2-receptor-distribution-in-guinea-pig-urinary-bladder-out-flow-region
#12
Na N Guan, Karl Svennersten, Petra J de Verdier, N Peter Wiklund, Lars E Gustafsson
The proximal urethra and urinary bladder trigone play important roles in continence. We have previously shown that PGD2 is released from guinea pig bladder urothelium/suburothelium and can inhibit detrusor contractile responses. We presently wished to investigate PGD2 actions in guinea pig out-flow region and the distribution of DP1 /DP2 receptors. The effects of PGD2 on urothelium-intact trigone and proximal urethra contractility were studied in organ bath experiments. Expression of DP1 /DP2 receptor proteins was analysed by western blot...
September 23, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27659399/adrenergic-signaling-elements-in-the-bladder-wall-of-the-adult-rat
#13
Sara Persyn, Jane Eastham, Stefan De Wachter, James Gillespie
A growing body of work is describing the absence of a significant sympathetic innervation of the detrusor implying little sympathetic regulation of bladder contractility. However, low doses of adrenergic agonists are capable of relaxing the bladder smooth muscle. If these effects underpin a physiological response then the cellular nature and operation of this system are currently unknown. The present immunohistochemistry study was done to explore the existence of alternative adrenergic signaling elements in the rat bladder wall...
September 10, 2016: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/27605581/testosterone-decreases-urinary-bladder-smooth-muscle-excitability-via-novel-signaling-mechanism-involving-direct-activation-of-the-bk-channels
#14
Kiril L Hristov, Shankar P Parajuli, Aaron Provence, Georgi V Petkov
In addition to improving sexual function, testosterone has been reported to have beneficial effects in ameliorating lower urinary tract symptoms by increasing bladder capacity and compliance, while decreasing bladder pressure. However, the cellular mechanisms by which testosterone regulates detrusor smooth muscle (DSM) excitability have not been elucidated. Here, we used amphotericin-B perforated whole cell patch-clamp and single channel recordings on inside-out excised membrane patches to investigate the regulatory role of testosterone in guinea pig DSM excitability...
December 1, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27596481/preserved-micturition-after-intradetrusor-onabotulinumtoxina-injection-for-treatment-of-neurogenic-bladder-dysfunction-in-parkinson-s-disease
#15
Stephanie C Knüpfer, Susanne A Schneider, Mareike M Averhoff, Carsten M Naumann, Günther Deuschl, Klaus-Peter Jünemann, Moritz F Hamann
BACKGROUND: To assess the efficacy and safety of intradetrusor onabotulinumtoxinA (OnabotA) injection treatment in patients with neurogenic lower urinary tract dysfunction (NLUTD), especially for patients with Parkinson disease (PD). METHODS: PD patients refractory to oral antimuscarinic participated in an off-label use study and were evaluated prior and after 200 IU OnabotA injection into detrusor muscle, including trigone. Changes due to treatment were evaluated using bladder diaries, urodynamics, and questionnaires...
2016: BMC Urology
https://www.readbyqxmd.com/read/27547465/the-challenges-in-the-diagnosis-of-detrusor-underactivity-in-clinical-practice-a-mini-review
#16
REVIEW
Ahmed Ahmed, Bilal Farhan, Simone Vernez, Gamal M Ghoniem
OBJECTIVE: To review the current definitions, terminology, epidemiology and aetiology of detrusor underactivity (DU), with specific attention to the diagnostic criteria in use. In addition, we address the relation and the overlap between DU and bladder outlet obstruction (BOO). In this mini-review, we hope to help identify DU patients and facilitate structured clinical evaluation and research. METHODS: We searched the English literature using ScienceDirect and PubMed for relevant articles...
September 2016: Arab Journal of Urology
https://www.readbyqxmd.com/read/27540361/netupitant-a-potent-and-highly-selective-nk1-receptor-antagonist-alleviates-acetic-acid-induced-bladder-overactivity-in-anesthetized-guinea-pigs
#17
Stefano Palea, Véronique Guilloteau, Moéz Rekik, Emanuela Lovati, Marc Guerard, Maria-Alba Guardia, Philippe Lluel, Claudio Pietra, Mitsuharu Yoshiyama
Introduction. Tachykinins potently contract the isolated urinary bladder from a number of animal species and play an important role in the regulation of the micturition reflex. On the guinea-pig isolated urinary bladder we examined the effects of a new potent and selective NK1 receptor antagonist (netupitant) on the contractions induced by a selective NK1 receptor agonist, SP-methylester (SP-OMe). Moreover, the effects of netupitant and another selective NK1 antagonist (L-733,060) were studied in anesthetized guinea-pigs using two experimental models, the isovolumetric bladder contractions and a model of bladder overactivity induced by intravesical administration of acetic acid (AA)...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27497848/functional-coupling-of-trpv4-channels-and-bk-channels-in-regulating-spontaneous-contractions-of-the-guinea-pig-urinary-bladder
#18
Ayu Isogai, Ken Lee, Retsu Mitsui, Hikaru Hashitani
We investigated the role of TRPV4 channels (TRPV4) in regulating the contractility of detrusor smooth muscle (DSM) and muscularis mucosae (MM) of the urinary bladder. Distribution of TRPV4 in DSM and MM of guinea-pig bladders was examined by fluorescence immunohistochemistry. Changes in the contractility of DSM and MM bundles were measured using isometric tension recording. Intracellular Ca(2+) dynamics were visualized by Cal-520 fluorescent Ca(2+) imaging, while membrane potential changes were recorded using intracellular microelectrode technique...
September 2016: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27494644/a-pilot-study-of-cardiac-electrophysiology-catheters-to-map-and-pace-bladder-electrical-activity
#19
Robert S Kelley, Michael D Vardy, Grant Simons, Henry Chen, Charles Ascher-Walsh, Michael Brodman
AIMS: This is a pilot study to evaluate the feasibility of using diagnostic cardiac electrophysiology catheters for recording intrinsic urinary bladder electrical activity and for electrical pacing capture of bladder tissue. METHODS: During cystoscopy, a curved quadripolar catheter was introduced and contact was made with the right and left halves of the dome and trigone in adult female patients undergoing cystoscopy. Electrical activity was recorded, using a commercially available cardiac electrophysiologic recording system, before and during pacing at 0...
August 5, 2016: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/27490041/functional-and-structural-changes-of-the-urinary-bladder-following-spinal-cord-injury-treatment-with-alpha-lipoic-acid
#20
Arif Ekiz, Zarife Nigâr Özdemir-Kumral, Mehmet Erşahin, Halil Tuğtepe, Ayliz Velioğlu Öğünç, Dilek Akakın, Demir Kıran, Derya Özsavcı, Necat Biber, Tayfun Hakan, Berrak Ç Yeğen, Göksel Şener, Hale Z Toklu
BACKGROUND & AIM: Alpha lipoic acid (LA) was shown to exert neuroprotection in trauma-induced spinal cord injury (SCI), which is frequently associated with urinary bladder complaints in patients with SCI. Accordingly, the protective effects of LA on biochemical and histological changes in bladder as well as functional studies were assessed. METHODS: Wistar albino rats were divided as control, SCI, and LA (50 mg/kg/day, ip) treated SCI groups (SCI+LA). The standard weight-drop (100 g/cm force at T10) method was used to induce a moderately severe SCI...
August 4, 2016: Neurourology and Urodynamics
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