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https://www.readbyqxmd.com/read/27867165/evaluation-of-a-new-motion-correction-algorithm-using-on-rigid-registration-in-respiratory-gated-pet-ct-images-of-liver-tumors
#1
Kei Wagatsuma, Tatsufumi Osawa, Naoki Yokokawa, Kenta Miwa, Keiichi Oda, Yoshiro Kudo, Yasushi Unno, Kimiteru Ito, Kenji Ishii
OBJECTIVE: The present study aimed to determine the qualitative and quantitative accuracy of the Q.Freeze algorithm in PET/CT images of liver tumors. METHODS: A body phantom and hot spheres representing liver tumors contained 5.3 and 21.2 kBq/mL of a solution containing (18)F radioactivity, respectively. The phantoms were moved in the superior-inferior direction at a motion displacement of 20 mm. Conventional respiratory-gated (RG) and Q.Freeze images were sorted into 6, 10, and 13 phase-groups...
2016: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/27660201/time-of-day-dependent-adaptation-of-the-hpa-axis-to-predictable-social-defeat-stress
#2
Christiane Koch, Manuela S Bartlang, Jana Thabea Kiehn, Lisa Lucke, Nadine Naujokat, Charlotte Helfrich-Förster, Stefan O Reber, Henrik Oster
In modern societies, the risk of developing a whole array of affective and somatic disorders is associated with the prevalence of frequent psychosocial stress. Therefore, a better understanding of adaptive stress responses and their underlying molecular mechanisms is of high clinical interest. In response to an acute stressor each organism can either show passive freezing or active fight-or-flight behavior, with activation of sympathetic nervous system and the hypothalamus-pituitary-adrenal (HPA) axis providing the necessary energy for the latter by releasing catecholamines and glucocorticoids (GC)...
September 22, 2016: Journal of Endocrinology
https://www.readbyqxmd.com/read/27129808/a-global-look-into-human-t-cell-subsets-before-and-after-cryopreservation-using-multiparametric-flow-cytometry-and-two-dimensional-visualization-analysis
#3
Jennifer Lemieux, Christine Jobin, Carl Simard, Sonia Néron
The cryopreservation of human lymphocytes is an essential step for the achievement of several cellular therapies. Besides, T cells are considered as promising actors in cancer therapy for their cytotoxic and regulatory properties. Consequently, the development of tools to monitor the impact of freezing and thawing processes on their fine distribution may be an asset to achieve quality control in cellular therapy. In this study, the phenotypes of freshly isolated human mononuclear cells were compared to those observed following one cycle of cryopreservation and rest periods 0h, 1h and 24h after thawing but before staining...
July 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/27021054/p-channel-ingan-gan-heterostructure-metal-oxide-semiconductor-field-effect-transistor-based-on-polarization-induced-two-dimensional-hole-gas
#4
Kexiong Zhang, Masatomo Sumiya, Meiyong Liao, Yasuo Koide, Liwen Sang
The concept of p-channel InGaN/GaN heterostructure field effect transistor (FET) using a two-dimensional hole gas (2DHG) induced by polarization effect is demonstrated. The existence of 2DHG near the lower interface of InGaN/GaN heterostructure is verified by theoretical simulation and capacitance-voltage profiling. The metal-oxide-semiconductor FET (MOSFET) with Al2O3 gate dielectric shows a drain-source current density of 0.51 mA/mm at the gate voltage of -2 V and drain bias of -15 V, an ON/OFF ratio of two orders of magnitude and effective hole mobility of 10 cm(2)/Vs at room temperature...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26950065/prediction-of-ovarian-hyperstimulation-syndrome-in-patients-treated-with-corifollitropin-alfa-or-rfsh-in-a-gnrh-antagonist-protocol
#5
Georg Griesinger, Pierre J M Verweij, Davis Gates, Paul Devroey, Keith Gordon, Barbara J Stegmann, Basil C Tarlatzis
STUDY QUESTION: What is the threshold for the prediction of moderate to severe or severe ovarian hyperstimulation syndrome (OHSS) based on the number of growing follicles ≥ 11 mm and/or estradiol (E2) levels? SUMMARY ANSWER: The optimal threshold of follicles ≥11 mm on the day of hCG to identify those at risk was 19 for both moderate to severe OHSS and for severe OHSS. Estradiol (E2) levels were less prognostic of OHSS than the number of follicles ≥ 11 mm...
2016: PloS One
https://www.readbyqxmd.com/read/25783771/dual-role-of-dopamine-d-2-like-receptors-in-the-mediation-of-conditioned-and-unconditioned-fear
#6
REVIEW
Marcus Lira Brandão, Amanda Ribeiro de Oliveira, Sangu Muthuraju, Ana Caroline Colombo, Viviane Mitsuko Saito, Teddy Talbot
A reduction of dopamine release or D2 receptor blockade in the terminal fields of the mesolimbic system, particularly the amygdala, clearly reduces conditioned fear. Similar D2 receptor antagonism in the neural substrates of fear in the midbrain tectum attenuates the processing of unconditioned aversive information. However, the implications of the interplay between opposing actions of dopamine in the rostral and caudal segments of the dopaminergic system are still unclear. Previous studies from this laboratory have reported the effects of dopaminergic drugs on behavior in rats in the elevated plus maze, auditory-evoked potentials (AEPs) recorded from the midbrain tectum, fear-potentiated startle, and conditioned freezing...
November 14, 2015: FEBS Letters
https://www.readbyqxmd.com/read/25564298/ctca-image-quality-improvement-by-using-snapshot-freeze-technique-under-prospective-and-retrospective-electrocardiographic-gating
#7
Lijuan Fan, Jiwang Zhang, Dongsheng Xu, Zhi Dong, Xu Li, Liren Zhang
BACKGROUND: Although coronary computed tomography angiography (CCTA) can detect coronary artery disease, limited temporal resolution of computed tomographic scanners may allow for motion artifacts, which may result in nonevaluable coronary segments. OBJECTIVE: This study aimed to assess Snapshot Freeze (SSF) Motion Correction algorithm for its effect on image quality of CCTA. METHODS AND RESULTS: Thirty patients underwent prospective electrocardiographic (ECG)-gating CCTA and 30 patients underwent retrospective ECG gating...
March 2015: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/25533484/nanoscale-distribution-of-presynaptic-ca-2-channels-and-its-impact-on-vesicular-release-during-development
#8
Yukihiro Nakamura, Harumi Harada, Naomi Kamasawa, Ko Matsui, Jason S Rothman, Ryuichi Shigemoto, R Angus Silver, David A DiGregorio, Tomoyuki Takahashi
Synaptic efficacy and precision are influenced by the coupling of voltage-gated Ca(2+) channels (VGCCs) to vesicles. But because the topography of VGCCs and their proximity to vesicles is unknown, a quantitative understanding of the determinants of vesicular release at nanometer scale is lacking. To investigate this, we combined freeze-fracture replica immunogold labeling of Cav2.1 channels, local [Ca(2+)] imaging, and patch pipette perfusion of EGTA at the calyx of Held. Between postnatal day 7 and 21, VGCCs formed variable sized clusters and vesicular release became less sensitive to EGTA, whereas fixed Ca(2+) buffer properties remained constant...
January 7, 2015: Neuron
https://www.readbyqxmd.com/read/25309814/cryopreservation-and-enumeration-of-human-endothelial-progenitor-and-endothelial-cells-for-clinical-trials
#9
T Bogoslovsky, D Wang, D Maric, L Scattergood-Keepper, M Spatz, S Auh, J Hallenbeck
BACKGROUND: Endothelial progenitor cells (EPC) are markers of endothelial injury and may serve as a surrogate marker for vascular repair in interventional clinical trials. Objectives of this study were to modify a method of isolation of peripheral blood mononuclear cells (PBMC) and enumeration of EPC and mature endothelial cells (EC) from peripheral blood and to evaluate influence of cryopreservation on viability of PBMC and on numbers of EPC and EC. PATIENTS/METHODS: EPC and EC were analyzed in healthy volunteers in freshly isolated PBMC collected in CPT (cell preparation tubes) and in PBMC cryopreserved with: 1) Gibco Recovery(™) Cell Culture Freezing Medium, 2) custom freezing medium...
September 20, 2013: Journal of Blood Disorders & Transfusion
https://www.readbyqxmd.com/read/25193780/effect-of-a-novel-motion-correction-algorithm-ssf-on-the-image-quality-of-coronary-cta-with-intermediate-heart-rates-segment-based-and-vessel-based-analyses
#10
Qianwen Li, Pengyu Li, Zhuangzhi Su, Xinyu Yao, Yan Wang, Chen Wang, Xiangying Du, Kuncheng Li
PURPOSE: To evaluate the effect of SnapShot Freeze (SSF) reconstruction at an intermediate heart-rate (HR) range (65-75bpm) and compare this method with single-sector reconstruction and bi-sector reconstruction on segmental and vessel bases in retrospective coronary computed tomography angiography (CCTA). MATERIALS AND METHODS: Retrospective electrocardiogram-gated CCTA was performed on 37 consecutive patients with HR between 65 and 75bpm using a 64-row CT scanner...
November 2014: European Journal of Radiology
https://www.readbyqxmd.com/read/25078614/hydraulic-performance-of-a-permeable-reactive-barrier-at-casey-station-antarctica
#11
K A Mumford, J L Rayner, I Snape, G W Stevens
A permeable bio-reactive barrier (PRB) was installed at Casey Station, Antarctica in 2005/06 to intercept, capture and degrade petroleum hydrocarbons from a decade old fuel spill. A funnel and gate configuration was selected and implemented. The reactive gate was split into five separate cells to enable the testing of five different treatment combinations. Although different treatment materials were used in each cell, each treatment combination contained the following reactive zones: a zone for the controlled release of nutrients to enhance degradation, a zone for hydrocarbon capture and enhanced degradation, and a zone to capture excess nutrients...
December 2014: Chemosphere
https://www.readbyqxmd.com/read/24950096/development-and-validation-of-an-lc-ms-ms-method-for-determination-of-the-l-type-voltage-gated-calcium-channel-nmda-receptor-antagonist-ngp1-01-in-mouse-serum
#12
Harini Jogiraju, Xiang Zhou, Ashta Lakshmi Prasad Gobburi, Kiran K Pedada, Werner J Geldenhuys, Cornelis J Van der Schyf, Samuel D Crish, David J Anderson
NGP1-01 (8-benzylamino-8,11-oxapentacyclo[5.4.0.0(2,6).0(3,10).0(5,9)]undecane) is a heterocyclic cage compound with multifunctional calcium channel blocking activity that has been demonstrated to be neuroprotective in several neurodegenerative models. A sensitive internal standard LC-MS/MS method was developed and validated to quantify NGP1-01 in mouse serum. The internal standard (IS) was 8-(2-phenylethylamino)-8,11-oxapentacyclo[5.4.0.0(2,6).0(3,10).0(5,9)]undecane. Sample preparation involved a protein precipitation procedure by addition of acetonitrile...
July 15, 2014: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/24880088/fluorescence-as-an-alternative-to-light-scatter-gating-strategies-to-identify-frozen-thawed-cells-with-flow-cytometry
#13
Anthony J F Reardon, Janet A W Elliott, Locksley E McGann
Flow cytometry is a key instrument in biological studies, used to identify and analyze cells in suspension. The identification of cells from debris is commonly based on light scatter properties as it has been shown that there is a relationship between forward scattered light and cell volume and this has become common practice in flow cytometry. Cryobiological conditions induce changes in cells that alter their light scatter properties. Cells with membrane damage from freeze-thaw stress produce lower forward scatter signals and may fall below standard forward scatter thresholds...
August 2014: Cryobiology
https://www.readbyqxmd.com/read/24651926/freezing-of-stressed-bilayers-and-vesicles
#14
Antti Lamberg, Takashi Taniguchi
We show using a minimalistic theoretical framework that phase transition decoupling in lipid bilayers is caused by a nonuniform stress profile due to an asymmetric distribution of lipids between the two leaflets. Applying this framework to vesicles, we demonstrate that their anomalous freezing is also caused by a stress asymmetry, but that this is due to lipid tail extension on freezing. Finally, we predict a previously unknown dependence of surface tension on temperature, find the phenomenon also in coarse grained molecular dynamics simulations, and suggest that it might have relevance in thermosensitive protein gating...
January 14, 2014: Soft Matter
https://www.readbyqxmd.com/read/24606584/distinct-axo-somato-dendritic-distributions-of-three-potassium-channels-in-ca1-hippocampal-pyramidal-cells
#15
Tekla Kirizs, Katalin Kerti-Szigeti, Andrea Lorincz, Zoltan Nusser
Potassium channels comprise the most diverse family of ion channels and play critical roles in a large variety of physiological and pathological processes. In addition to their molecular diversity, variations in their distributions and densities on the axo-somato-dendritic surface of neurons are key parameters in determining their functional impact. Despite extensive electrophysiological and anatomical investigations, the exact location and densities of most K(+) channels in small subcellular compartments are still unknown...
June 2014: European Journal of Neuroscience
https://www.readbyqxmd.com/read/24050525/nicotinic-acetylcholine-receptor-and-the-structural-basis-of-neuromuscular-transmission-insights-from-torpedo-postsynaptic-membranes
#16
REVIEW
Nigel Unwin
The nicotinic acetylcholine (ACh) receptor, at the neuromuscular junction, is a neurotransmitter-gated ion channel that has been fine-tuned through evolution to transduce a chemical signal into an electrical signal with maximum efficiency and speed. It is composed from three similar and two identical polypeptide chains, arranged in a ring around a narrow membrane pore. Central to the design of this assembly is a hydrophobic gate in the pore, more than 50 Å away from sites in the extracellular domain where ACh binds...
November 2013: Quarterly Reviews of Biophysics
https://www.readbyqxmd.com/read/23557598/sources-of-heterogeneity-in-human-monocyte-subsets
#17
Laura J Appleby, Norman Nausch, Nicholas Midzi, Takafira Mduluza, Judith E Allen, Francisca Mutapi
Human monocytes are commonly defined and discriminated by the extent of their cell surface expression of CD14 and CD16, with associated differences in function and phenotype related to the intensity of expression of these markers. With increasing interest into the function and behaviour of monocytes, it is important to have a clear understanding of how differing strategies of analysis can affect results and how different protocols and population backgrounds can affect this highly morphogenic cell type. Using PBMCs from populations with differing ethnicities and histories of parasite exposure we have characterized monocyte phenotype based on intensity of CD14 and CD16 expression...
April 2013: Immunology Letters
https://www.readbyqxmd.com/read/23452203/evaluation-of-microparticles-in-whole-blood-by-multicolour-flow-cytometry-assay
#18
Christina Christersson, Matilda Johnell, Agneta Siegbahn
OBJECTIVE: To develop and evaluate a multicolour flow cytometry method for analysis of microparticles (MPs) in fresh whole blood without any centrifugation steps or freezing/thawing procedure. MATERIALS AND METHODS: Flow cytometry was performed using a FC500 MPL cytometer. The compensation in the protocol was performed based on the platelet population. Polystyrene microspheres 0.50-1.27 μm were used for size position, and the MP gate was set as particles 0.5-1...
April 2013: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/23071265/charge-movement-in-gating-locked-hcn-channels-reveals-weak-coupling-of-voltage-sensors-and-gate
#19
Sujung Ryu, Gary Yellen
HCN (hyperpolarization-activated cyclic nucleotide gated) pacemaker channels have an architecture similar to that of voltage-gated K(+) channels, but they open with the opposite voltage dependence. HCN channels use essentially the same positively charged voltage sensors and intracellular activation gates as K(+) channels, but apparently these two components are coupled differently. In this study, we examine the energetics of coupling between the voltage sensor and the pore by using cysteine mutant channels for which low concentrations of Cd(2+) ions freeze the open-closed gating machinery but still allow the sensors to move...
November 2012: Journal of General Physiology
https://www.readbyqxmd.com/read/22954345/robust-multi-parameter-single-platform-quantification-of-myeloid-and-b-lymphoid-cd34-progenitor-cells-in-all-clinical-cd34-cell-sources-and-in-thawed-pbsc
#20
Gerhard Fritsch, Volker Witt, Hans Peter Spengler, Josefa Pichler, Daniela Scharner, Elke Zipperer, Dijana Trbojevic, Julia Stemberger, René Geyeregger, Muy-Kheng Maria Tea, Dieter Printz
As B-lymphoid progenitor cells do not give rise to in vitro colony formation and are unlikely to support myeloid engraftment, we validated a five-color extension of the single platform Stem Cell Enumeration (SCE) kit, to routinely quantify myeloid and B-lymphoid progenitor cells. Fresh samples (n > 20 each) of granulocyte colony stimulating factor mobilized blood (peripheral blood (PB)), cord blood (CB), bone marrow (BM), and apheresis products (APs) were stained in TruCOUNT™ tubes and the results were compared with those from the two-color CD45/CD34 reagent combination and the three-color SCE kit...
October 2012: Pediatric Hematology and Oncology
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