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https://www.readbyqxmd.com/read/29785455/robotic-assisted-laparoscopic-median-arcuate-ligament-release-7-year-experience-from-a-single-tertiary-care-center
#1
Usah Khrucharoen, Yen-Yi Juo, Yas Sanaiha, Yijun Chen, Juan C Jimenez, Erik P Dutson
BACKGROUND: Despite previous reports of robotic-assisted laparoscopic release for median arcuate ligament syndrome (MALS), the safety and efficacy profile of this approach has been difficult to establish due to the rarity of this diagnostic entity. We aim to present our experience from a tertiary minimally invasive surgery referral center. METHODS: A case series was performed whereby all patients who underwent robotic-assisted MAL release from July 2010 to July 2017 at our institution were included...
May 21, 2018: Surgical Endoscopy
https://www.readbyqxmd.com/read/29784566/preoperative-point-of-care-ultrasound-and-its-impact-on-arteriovenous-fistula-maturation-outcomes
#2
Sajjid Hossain, Amit Sharma, Luc Dubois, Guy DeRose, Audra Duncan, Adam H Power
OBJECTIVE: Duplex ultrasound as a preoperative assessment tool in the clinic may help identify anatomic factors predictive of fistula maturation. Preoperative point-of-care ultrasound (POCUS) offers surgeons an alternative to routine formal vein mapping as it can be performed by the operator during the initial clinic visit. We sought to determine the impact of POCUS as an adjunct to physical examination on arteriovenous fistula maturation. METHODS: All consecutive patients undergoing first-time dialysis access creation from December 2007 to December 2014 were retrospectively reviewed...
May 18, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29784364/protein-binding-protection-in-combination-with-dna-masking-for-sensitive-and-reliable-transcription-factor-detection
#3
Xiaowen Xu, Lei Wang, Desong Zhu, Yan Wang, Wei Jiang
Sensitive and reliable detection of transcription factors (TFs) is crucial for disease diagnosis and drug discovery. Herein, a protein binding protection in combination with DNA masking-based strategy is developed for sensitive and reliable detection of NF-κB p50. First, NF-κB p50 binds specifically to a hairpin probe to form a protein-DNA complex. Then, the formed complex protects the hairpin probe from hybridization with the masking strand and subsequently triggers the cascade signal amplification of strand displacement amplification (SDA) and exponential rolling circle amplification (ERCA)...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784358/short-probe-based-duplex-specific-nuclease-signal-amplification-strategy-enables-imaging-of-endogenous-micrornas-in-living-cells-with-ultrahigh-specificity
#4
Yingjun Ma, Jun Chen, Danping Chen, Yuzhi Xu, Li Zhang, Zong Dai, Xiaoyong Zou
Specific nucleic acids amplification at a constant and mild temperature is important for imaging assay of endogenous microRNAs (miRNAs) in living cells. Duplex-specific nuclease (DSN) is attractive in one-step isothermal assay of miRNA; however, its intrinsic limitations of low amplification specificity and high reaction temperature greatly restrict the application scope. Herein, we present a short-probe-based DSN signal amplification (spDSNSA) strategy enabling analysis of miRNAs at body temperature with significantly high specificity...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29783914/early-application-of-an-intermittent-pneumatic-compression-device-is-safe-and-results-in-proximal-arteriovenous-fistula-enlargement
#5
Sanjay Desai, Amit Mitra, Ed Arkans, Tej M Singh
INTRODUCTION: Delays in arteriovenous fistula maturation can cause care delays and increased costs. Increased distention pressure and intermittent wall shear stress may dilate veins based on prior research. Early use of non-invasive devices may help assist clinical arteriovenous fistula dilation. METHODS: This was an Institutional Review Board approved study. After arteriovenous fistula creation, a novel, intermittent pneumatic compression device (Fist Assist® ) was applied 15 cm proximal to arteriovenous fistula enabling 60 mmHg of cyclic compression for 6 h daily for 30 days...
May 1, 2018: Journal of Vascular Access
https://www.readbyqxmd.com/read/29781902/endovenous-thermal-ablation-of-recurrent-varicose-veins-due-to-residual-great-saphenous-venous-insufficiency-after-saphenous-venous-surgery-a-comparative-study
#6
Jin Ho Hwang, Sang Woo Park, Il Soo Chang, Ki Hyun Kim, Ji Hun Kang
BACKGROUND: Redo surgery for recurrent varicose veins of the great saphenous vein (GSV) is technically more challenging than the initial surgery. OBJECTIVE: To compare 980 and 1,470-nm endovenous laser ablation (EVLA) and radiofrequency ablation (RFA) for the treatment of residual GSV insufficiency after saphenofemoral ligation ± stripping. MATERIALS AND METHODS: Thirty-seven limbs in 29 patients with recurrent varicose veins were retrospectively evaluated...
May 16, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29781769/the-laparoscopic-management-of-median-arcuate-ligament-syndrome-and-its-long-term-outcomes
#7
Henry D De'Ath, Simon Wong, Karoly Szentpali, Shaw Somers, Tom Peck, Christian H Wakefield
BACKGROUND: Case reports and small series of the surgical and radiological management of median arcuate ligament syndrome (MALS) have been described, however, long-term outcome data are lacking. The purpose of this study was to review our experience of the laparoscopic management of MALS, and describe the long-term outcomes after surgical intervention. METHODS: Data were collected between 2005 and 2016 in a single U.K. institution. All patients with MALS who underwent laparoscopic decompression of the celiac artery were included...
May 21, 2018: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/29781489/probing-the-competition-between-duplex-and-g-quadruplex-i-motif-structures-using-a-conformation-sensitive-fluorescent-nucleoside-probe
#8
Pramod M Sabale, Arun A Tanpure, Seergazhi G Srivatsan
Double-stranded segments of a genome that can potentially form G-quadruplex (GQ) and/or i-motif (iM) structures are considered to be important regulatory elements. Hence, the development of a common probe that can detect GQ and iM structures and also distinguish them from a duplex structure will be highly useful in understanding the propensity of such segments to adopt duplex or non-canonical four-stranded structures. Here, we describe the utility of a conformation-sensitive fluorescent nucleoside analog, which was originally developed as a GQ sensor, in detecting the iM structures of C-rich DNA oligonucleotides (ONs)...
May 21, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29781153/value-of-duplex-doppler-ultrasonography-for-the-evaluation-of-dysfunctional-hemodialysis-access-arteriovenous-fistulas-prior-to-endovascular-interventions
#9
Isik Conkbayir, Pinar Celtikci, Onur Ergun, Hasan Ali Durmaz
PURPOSE: To demonstrate accuracy of duplex Doppler ultrasonography (DUS) for the evaluation of native-vein arteriovenous fistula (AVF) dysfunction in end-stage renal disease patients. METHODS: This retrospective study included 60 patients with dysfunctioning native-vein AVF evaluated with DUS and digital subtraction angiography (DSA) whose results were compared for detection, identification, and localization of AVF lesions. We calculated sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of DUS and evaluated the agreement between DUS and DSA by the Cohen's kappa coefficient (κ)...
May 21, 2018: Journal of Clinical Ultrasound: JCU
https://www.readbyqxmd.com/read/29781111/assessment-of-hemodynamic-alterations-in-cerebral-veins-in-patients-with-intracerebral-hemorrhage-using-duplex-color-coded-sonography
#10
Zhan-Qiang Jin, Hao Feng, Wen He, Xing-Quan Zhao
OBJECTIVES: To investigate the contributions of hemodynamic alterations in cerebral veins to the prognosis of patients with primary intracerebral hemorrhage (ICH). METHODS: Duplex color-coded sonography was performed on 87 ICH patients between July 2016 and October 2017. The time-averaged peak velocity, the time-averaged mean velocity (TAMV), the blood flow volume (BFV) of bilateral internal jugular veins (IJVs) and vertebral veins (VVs), and the mean velocities of bilateral basal veins of Rosenthal, straight sinus, and bilateral transverse sinuses were calculated to understand the relationship of hemodynamic data with prognosis in patients with ICH...
May 21, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/29781024/the-owl-sensor-a-fragile-dna-nanostructure-for-the-analysis-of-single-nucleotide-variations
#11
Rebekah J Karadeema, Maria Stancescu, Tyler P Steidl, Sophia C Bertot, Dmitry M Kolpashchikov
Analysis of single nucleotide variations (SNVs) in DNA and RNA sequences is instrumental in healthcare for the detection of genetic and infectious diseases and drug-resistant pathogens. Here we took advantage of the developments in DNA nanotechnology to design a hybridization sensor, named the 'owl sensor', which produces a fluorescence signal only when it complexes with fully complementary DNA or RNA analytes. The novelty of the owl sensor operation is that the selectivity of analyte recognition is, at least in part, determined by the structural rigidity and stability of the entire DNA nanostructure rather than exclusively by the stability of the analyte-probe duplex, as is the case for conventional hybridization probes...
May 21, 2018: Nanoscale
https://www.readbyqxmd.com/read/29781012/microfluidic-curved-channel-centrifuge-for-solution-exchange-of-target-microparticles-and-their-simultaneous-separation-from-bacteria
#12
Pouriya Bayat, Pouya Rezai
One of the common operations in sample preparation is to separate specific particles (e.g. target cells, embryos or microparticles) from non-target substances (e.g. bacteria) in a fluid and to wash them into clean buffers for further processing like detection (called solution exchange in this paper). For instance, solution exchange is widely needed in preparing fluidic samples for biosensing at the point-of-care and point-of-use, but still conducted via the use of cumbersome and time-consuming off-chip analyte washing and purification techniques...
May 21, 2018: Soft Matter
https://www.readbyqxmd.com/read/29780486/glucose-nucleobase-pairs-within-dna-impact-of-hydrophobicity-alternative-linking-unit-and-dna-polymerase-nucleotide-insertion-studies
#13
Empar Vengut-Climent, Pablo Peñalver, Ricardo Lucas, Irene Gómez-Pinto, Anna Aviñó, Alicia M Muro-Pastor, Elsa Galbis, M Violante de Paz, Célia Fonseca Guerra, F Matthias Bickelhaupt, Ramón Eritja, Carlos González, Juan Carlos Morales
Recently, we studied glucose-nucleobase pairs, a binding motif found in aminoglycoside-RNA recognition. DNA duplexes with glucose as a nucleobase were able to hybridize and were selective for purines. They were less stable than natural DNA but still fit well on regular B-DNA. These results opened up the possible use of glucose as a non-aromatic DNA base mimic. Here, we have studied the incorporation and thermal stability of glucose with different types of anchoring units and alternative apolar sugar-nucleobase pairs...
April 14, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780226/cerebral-border-zone-infarction-an-etiological-study
#14
Tarek Mohammed El-Gammal, Wafik Said Bahnasy, Osama Abd Allah Ragab, Ayman Mohammed Al-Malt
Background: Border zone infarcts (BZI) are ischemic lesions at the junction between two main arterial territories which may be either cortical or internal BZI. Methods: This study was conducted on 76 cerebral BZI patients and 20 healthy control subjects. Patients were divided to group I included 26 internal BZI, group II included 19 cortical BZI and group III included 21 mixed internal/cortical BZI patients. Included subjects were submitted to neurological examination, laboratory investigations, ECG, echocardiogram, brain CT and/or MRI and extra and intracranial blood vessels imaging by duplex and CT angiography...
2018: The Egyptian journal of neurology, psychiatry and neurosurgery
https://www.readbyqxmd.com/read/29779371/detection-of-nucleic-acids-in-complex-samples-via-magnetic-microbead-assisted-catalyzed-hairpin-assembly-and-dd-a-fret
#15
Hongmei Fang, Nuli Xie, Min Ou, Jin Huang, Wenshan Li, Qing Wang, Jianbo Liu, Xiaohai Yang, Kemin Wang
Nucleic acids, as one kind of significant biomarkers, have attracted tremendous attention and exhibited immense value in fundamental studies and clinical applications. In this work, we developed a fluorescent assay for detecting nucleic acids in complex samples based on magnetic microbead (MMB)-assisted catalyzed hairpin assembly (CHA) and donor donor-acceptor fluorescence resonance energy transfer ("DD-A" FRET) signaling mechanism. Three types of DNA hairpin probes were employed in this system, including Capture, H1 (double FAM-labelled probe as FRET donor) and H2 (TAMRA-labelled probe as FRET acceptor)...
May 21, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29779369/quantitative-glycomic-analysis-by-mass-defect-based-dimethyl-pyrimidinyl-ornithine-dipyro-tags-and-high-resolution-mass-spectrometry
#16
Bingming Chen, Yu Feng, Dustin C Frost, Xuefei Zhong, Amanda R Buchberger, Jillian Johnson, Meng Xu, Miriam Kim, Diane Puccetti, Carol Diamond, Chrysanthy Ikonomidou, Lingjun Li
We recently developed a novel amine-reactive mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), for quantitative proteomic analysis at the MS1 level. In this work, we further extend the application of the DiPyrO tag, which provides amine group reactivity, optical detection capability, and improved electrospray sensitivity, to quantify N-linked glycans enzymatically released from glycoproteins in the glycosylamine form. Duplex DiPyrO tags that differ in mass by 45.3 mDa were used to label the glycosylamine moieties of freshly released N-glycosylamines from glycoprotein standards and human serum proteins...
May 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29775857/enzyme-free-homogeneous-electrochemical-biosensor-for-dna-assay-using-toehold-triggered-strand-displacement-reaction-coupled-with-host-guest-recognition-of-fe-3-o-4-sio-2-%C3%AE-cd-nanocomposites
#17
Jingjing Jiang, Xinyi Lin, Dong Ding, Guowang Diao
Taking advantages of the toehold-triggered strand displacement reaction (TSDR) and host-guest interaction of β-cyclodextrin (β-CD), a facile enzyme-free and homogeneous electrochemical sensing strategy was designed for the sensitive assay of target DNA using Fe3 O4 @SiO2 @β-CD nanocomposites and ferrocene-labeled hairpin DNA (H-1) as the capture and electrochemical probes, respectively. Upon addition of target molecule, the initiated TSDR process induced the conformational change of H-1, and subsequently stimulated the dynamic assembly of assist probes (A-1 and A-2) to generate H-1:A-1:A-2 duplex along with the release of target sequence...
April 17, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29774900/multiplex-detection-of-quality-indicator-molecule-targets-in-urine-using-programmable-hairpin-probes-based-on-a-simple-double-t-type-microchip-electrophoresis-platform-and-isothermal-polymerase-catalyzed-target-recycling
#18
Lingying Zhou, Ning Gan, Yongxiang Wu, Futao Hu, Jianyuan Lin, Yuting Cao, Dazhen Wu
Recently, it has been crucial to be able to detect and quantify small molecular targets simultaneously in biological samples. Herein, a simple and conventional double-T type microchip electrophoresis (MCE) based platform for the multiplex detection of quality indicator molecule targets in urine, using ampicillin (AMPI), adenosine triphosphate (ATP) and estradiol (E2) as models, was developed. Several programmable hairpin probes (PHPs) were designed for detecting different targets and triggering isothermal polymerase-catalyzed target recycling (IPCTR) for signal amplification...
May 18, 2018: Analyst
https://www.readbyqxmd.com/read/29772723/evolution-of-grain-interfaces-in-annealed-duplex-stainless-steel-after-parallel-cross-rolling-and-direct-rolling
#19
Ming Wang, Haoqing Li, Yujing Tian, Hong Guo, Xiaoying Fang, Yuebin Guo
Changes in various grain interfaces, including the grain boundary and phase boundary, are a strong indication of microstructural changes, particularly ultra-fined grains achieved by large strain deformation and subsequent annealing. After direct rolling and cross rolling with the same strain of ε = 2, the distributions of the interfaces in annealed UNS S32304 duplex stainless steel were investigated using electron backscatter diffraction (EBSD) in this study. The ferrite experienced continued recovery, and a high density of low-angle grain boundaries (LAGBs) was produced...
May 16, 2018: Materials
https://www.readbyqxmd.com/read/29772375/duplex-functional-g-quadruplex-nmm-fluorescent-probe-for-label-free-detection-of-lead-ii-and-mercury-ii-ions
#20
Qian Zhu, Lanhua Liu, Yunpeng Xing, Xiaohong Zhou
To meet the severe water pollution status, multi-target biosensor becomes one of research focus. We herein report a novel duplex functional fluorescent biosensor for the label-free and separate detection of Pb2+ and Hg2+ with high sensitivity and selectivity. A K+ -induced fluorescent G-quadruplex probe was assembled by a guanine-rich sequence (AGRO100) and N-methyl mesoporphyrin IX(NMM). It changed into a more stably non-fluorescent G-quadruplex structure and a hairpin-like structure upon binding Pb2+ and Hg2+ ions, respectively...
May 1, 2018: Journal of Hazardous Materials
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