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https://www.readbyqxmd.com/read/29150366/optimal-ultrasound-criteria-for-grading-stenosis-of-the-superficial-femoral-artery
#1
Mingjie Gao, Yang Hua, Xinyu Zhao, Lingyun Jia, Jie Yang, Beibei Liu
This retrospective study determined the duplex ultrasound scanning criteria for detecting 50%-69% and 70%-99% stenosis of the superficial femoral artery (SFA). Examinations of 278 limbs in 185 patients with peripheral arterial disease were performed. Duplex ultrasound scanning was used to measure the diameter of the vascular lumen, the peak systolic velocity (PSV) at the stenotic segment of the SFA (PSVst), the segment proximal to the stenosis (PSVpro) and the popliteal artery (PSVpop, distal to the stenosis)...
November 14, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/29149299/twj-screen-an-isothermal-screening-assay-to-assess-ligand-dna-junction-interactions-in-vitro
#2
Ludivine Guyon, Marc Pirrotta, Katerina Duskova, Anton Granzhan, Marie-Paule Teulade-Fichou, David Monchaud
The quest for chemicals able to operate at selected genomic loci in a spatiotemporally controlled manner is desirable to create manageable DNA damages. Mounting evidence now shows that alternative DNA structures, including G-quadruplexes and branched DNA (or DNA junctions), might hamper proper progression of replication fork, thus triggering DNA damages and genomic instability. Therefore, small molecules that stabilize these DNA structures are currently scrutinized as a promising way to create genomic defects that cannot be dealt with properly by cancer cells...
November 15, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29148817/specific-recognition-of-g-quadruplexes-over-duplex-dna-by-a-macromolecular-nir-two-photon-fluorescent-probe
#3
Marco Deiana, Bastien Mettra, Lara Martinez-Fernandez, Leszek Mateusz Mazur, Krzysztof Pawlik, Chantal Andraud, Marek Samoc, Roberto Improta, Cyrille Monnereau, Katarzyna Matczyszyn
The implication of guanine-rich DNA sequences in biologically important roles such as telomerase dysfunction and the regulation of gene expression has prompted the search for structure-specific G-quadruplex agents for targeted diagnostic and therapeutic applications. To date, the number of two-photon absorbing fluorogenic dyes with intrinsically large two-photon absorption (TPA) cross-section (σ2) and quantum yield (φf), capable of specific detection of G-motifs over duplex DNAs is still limited. Herein, we report on a NIR two-photon poly(cationic) anthracene-based macromolecule able to selectively target G-quadruplexes (G4s) over genomic double-stranded DNA...
November 17, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29148714/evaluating-the-rate-and-substrate-specificity-of-laboratory-evolved-xna-polymerases
#4
Ali Nikoomanzar, Matthew R Dunn, John C Chaput
Engineered polymerases that can copy genetic information between DNA and xeno-nucleic acids (XNA) hold tremendous value as reagents in future bio-technology applications. However, current XNA poly-merases function with inferior activity relative to their natural counterparts, indicating that current polymerase engineering efforts would benefit from new benchmark-ing assays. Here, we describe a highly parallel, low cost method for measuring the average rate and substrate specificity of XNA polymerases in a standard qPCR instrument...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148297/detection-of-pseudorabies-virus-by-duplex-droplet-digital-pcr-assay
#5
Meishen Ren, Hua Lin, Shijie Chen, Miao Yang, Wei An, Yin Wang, Changhua Xue, Yinjie Sun, Yubao Yan, Juan Hu
Aujeszky's disease, caused by pseudorabies virus (PRV), has damaged the economy of the Chinese swine industry. A large number of PRV gene-deleted vaccines have been constructed based on deletion of the glycoprotein E ( gE) gene combined with other virulence-related gene deletions, such as thymidine kinase ( TK), whereas PRV wild-type strains contain an intact gE gene. We developed a sensitive duplex droplet digital PCR (ddPCR) assay to rapidly detect PRV wild-type isolates and gE gene-deleted viral vaccines...
November 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29148095/vascular-complications-after-percutaneous-mitral-valve-repair-and-venous-access-closure-using-suture-or-closure-device
#6
Birgit Steppich, Felix Stegmüller, Philipp Moritz Rumpf, Jürgen Pache, Carolin Sonne, Hasema Lesevic, Daniel Braun, Jörg Hausleiter, Albert Markus Kasel, Ilka Ott
OBJECTIVE: The aim of this study was to assess the impact of different access-site closure strategies, suture or closure device (Proglide, Abbott Vascular), on vascular and bleeding complications after percutaneous mitral valve repair (MitraClip, Abbott Vascular). BACKGROUND: Considering the high-risk profile in patients receiving percutaneous mitral valve repair, complications related to the large 24 Fr access sheath and its relation to the closure technique have not been evaluated so far...
November 16, 2017: Journal of Interventional Cardiology
https://www.readbyqxmd.com/read/29147997/drug-eluting-balloon-angioplasty-for-assisted-maturation-of-failing-fistulae
#7
Alexandros Mallios, Jeffrey Hull, Benoit Boura, Alessandro Costanzo, Myriam Combes
OBJECTIVE: To present our experience of balloon-assisted maturation with drug-eluting balloon dilation in patients with recurrent failing arteriovenous fistulae. CASE SERIES: Three patients (all males, mean age 71 years) with a complex history of failed attempts at native fistula creation underwent surgical creation of arteriovenous fistulae. Two patients had a two-stage brachio-brachial fistula and 1 had a brachio-cephalic fistula that also required subsequent elevation...
November 13, 2017: Journal of Vascular Access
https://www.readbyqxmd.com/read/29147566/diagnosis-of-stenosis-within-the-popliteal-femoral-venous-segment-upon-clinical-presentation-with-a-venous-ulcer-and-subsequent-successful-treatment-with-venoplasty
#8
Emma Dabbs, Alina Sheikh, David Beckett, Mark S Whiteley
This case study reports the diagnosis and treatment of a lower limb venous ulcer with abnormal underlying venous pathology. One male patient presented with bilateral varicose veins and a right lower limb ulcer. Upon investigation, full-leg duplex ultrasonography revealed total incompetence of the great saphenous vein in the left leg. In the right leg, duplex ultrasonography showed proximal incompetence of the small saphenous vein, and dilation of the anterior accessory saphenous vein, which remained competent...
2017: SAGE open medical case reports
https://www.readbyqxmd.com/read/29146924/interpretation-of-dynamic-tensile-behavior-by-austenite-stability-in-ferrite-austenite-duplex-lightweight-steels
#9
Jaeyeong Park, Min Cheol Jo, Hyeok Jae Jeong, Seok Su Sohn, Jai-Hyun Kwak, Hyoung Seop Kim, Sunghak Lee
Phenomena occurring in duplex lightweight steels under dynamic loading are hardly investigated, although its understanding is essentially needed in applications of automotive steels. In this study, quasi-static and dynamic tensile properties of duplex lightweight steels were investigated by focusing on how TRIP and TWIP mechanisms were varied under the quasi-static and dynamic loading conditions. As the annealing temperature increased, the grain size and volume fraction of austenite increased, thereby gradually decreasing austenite stability...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29145633/the-initiation-propagation-and-dynamics-of-crispr-spycas9-r-loop-complex
#10
Yan Zeng, Yang Cui, Yong Zhang, Yanruo Zhang, Meng Liang, Hui Chen, Jie Lan, Guangtao Song, Jizhong Lou
CRISPR-Cas9 system has been widely used for efficient genome editing. Although the structures of Cas9 protein in complex with single-guided RNA (sgRNA) and target DNA have been resolved, the molecular details about the formation of Cas9 endonuclease R-loop structure remain elusive. Here we examine the DNA cleavage activities of Streptococcus pyogenes Cas9 (SpyCas9) and its mutants using various target sequences and study the conformational dynamics of R-loop structure during target binding using single-molecule fluorescence energy transfer (smFRET) technique...
November 14, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29145350/ultrasonographic-evaluation-of-complications-related-to-transfemoral-arterial-procedures
#11
Eun Ju Chun
The transfemoral arterial approach is used to gain access for angiography, percutaneous coronary interventions, or various endovascular therapies. To decrease the risk of procedure-related vascular complications, it is recommended to puncture the common femoral artery in its middle segment. However, due to inadequate access or anatomical variability, various complications, including hematoma, pseudoaneurysm, arteriovenous fistula, thrombosis, or dissection, can occur after transfemoral arterial interventions...
August 25, 2017: Ultrasonography
https://www.readbyqxmd.com/read/29145074/ratiometric-electrochemical-assay-for-sensitive-detecting-microrna-based-on-dual-amplification-mechanism-of-duplex-specific-nuclease-and-hybridization-chain-reaction
#12
Yan-Hong Yuan, Bao-Zhu Chi, Shao-Hua Wen, Ru-Ping Liang, Zhi-Mei Li, Jian-Ding Qiu
We propose a ratiometric electrochemical assay for detecting microRNA (miRNA) on the basis of dual-amplification mechanism by using distinguishable electrochemical signals from thionine (Thi) and ferrocene (Fc). The thiol-modified and ferrocene-labeled hairpin capture probes (CP) are first immobilized on an Au electrode via Au-S reaction. The target miRNA hybridizes with CP and unfolding the hairpin structure of CP to form miRNA-DNA duplexes. Then, kamchatka crab duplex specific nuclease (DSN) specifically cleaves the DNA in miRNA-DNA duplexes, leading to the release of miRNA and another cleaves cycle, meanwhile, numerous Fc leaves away from the electrode surface and leads to the signal-off of Fc...
November 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29144760/multi-spectroscopic-and-theoretical-exploration-on-the-comparative-binding-aspects-of-the-bio-flavonoid-fisetin-with-triple-and-double-helical-forms-of-rna
#13
Sutanwi Bhuiya, Lucy Haque, Rapti Goswami, Suman Das
The interactions of RNA triplex (U.A*U) and duplex (A.U) with the naturally occurring flavonoid fisetin (FTN) have been examined at pH 7.0 using various spectroscopic, viscometric and theoretical studies. Experimental observations showed that the ligand binds with both double and triple helical forms of RNA, although the binding affinity is greater for the triplex structure (5.94106 M-1) compared to the duplex counterpart (1.0105 M-1). Thermal melting experiments revealed that the Hoogsteen base paired third strand of triplex was stabilized to a greater extent (~14 C) compared to the Watson-Crick base paired second strand (~4 C) in presence of FTN...
November 16, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29144136/photophysics-of-j-aggregate-mediated-energy-transfer-on-dna
#14
James L Banal, Toru Kondo, Rémi Veneziano, Mark Bathe, Gabriela S Schlau-Cohen
Achieving nanoscale spatial and electronic control over the formation of dye aggregates is a major synthetic challenge due to their typically inhomogeneous self-assembly, which limits control over their higher-order organization. To address these challenges, synthetic DNA-templated pseudoisocyanine (PIC) J-aggregates were recently introduced. However, the dependence of the photophysics of the superradiant exciton on the underlying DNA template length and the impact of static disorder on energy transfer through these PIC J-aggregates remain unknown...
November 16, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29142342/prevalence-of-subclinical-peripheral-vascular-disease-in-obese-egyptian-patients
#15
Jehan Ahmed Younes, Nahla Dessoki El-Sayed, Ayman Ismail Kamel
Objective: To detect subclinical peripheral vascular disease in obese Egyptian patients and establish relations between obesity, metabolic risk factors, and PVD. Methods: This was a prospective case-control study including 100 obese patients (BMI >30) (G1). In addition, 100 age and sex matched non-obese healthy subjects as a control group (G2). Both groups were subjected to duplex ultrasound, Radionuclide muscle scan. Angiography was done for 17 patients. Results: The image pattern of 99mTc-MIBI muscles uptake was studied and perfusion reserve (PR%) was calculated in (G1) and (G2)...
October 2017: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
https://www.readbyqxmd.com/read/29142126/hpv-replication-regulation-by-acetylation-of-a-conserved-lysine-in-the-e2-protein
#16
Yanique Thomas, Elliot J Androphy
The Papillomavirus (PV) E2 protein is a sequence specific DNA binding protein that recruits cellular factors to its genome in infected epithelial cells. E2 also binds to and loads the viral E1 DNA helicase at the origin of replication. Post-translational modifications (PTMs) of PV E2 have been identified as potential regulators E2 functions. We recently reported lysine (K) 111 as a target of p300 acetylation in bovine (B)PV. The di-lysines at 111 and 112 are conserved in almost all papillomaviruses. We pursued a mutational approach to query the functional significance of lysine in human (H)PV E2...
November 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29141408/accurate-quantification-of-nucleic-acids-using-hypochromicity-measurements-in-conjunction-with-uv-spectrophotometry
#17
Alison Nwokeoji, Peter M Kilby, David Portwood, Mark J Dickman
UV absorbance spectrophotometry is widely used for the quantification of nucleic acids. For accurate quantification it is important to determine the hypochromocity of the oligonucleotide or complex nucleic acid structure. The use of thermal denaturation studies in conjunction with UV spectrophotometry to determine hypochromicity requires prolonged, elevated temperatures, which may cause partial hydrolysis of RNA. In addition, dsRNA is difficult to denature even at elevated temperature and the extinction coefficients of nucleic acids are also affected by temperature, which makes it difficult to accurately determine the nucleic acid concentration...
November 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29137566/a-novel-exercise-device-for-venous-thromboembolism-prophylaxis-improves-venous-flow-in-bed-versus-ankle-movement-exercises-in-healthy-volunteers
#18
Yukiyo Shimizu, Hiroshi Kamada, Masataka Sakane, Shizu Aikawa, Hirotaka Mutsuzaki, Kenta Tanaka, Hajime Mishima, Akihiro Kanamori, Tomofumi Nishino, Naoyuki Ochiai, Masashi Yamazaki
PURPOSE: Venous thromboembolism prophylaxis is crucial. To facilitate active ankle movement in postoperative and bedridden patients, we developed a novel leg exercise apparatus (LEX). We investigated the effect of the LEX by comparing increases in lower extremity venous flow during different modes of exercise using the LEX. METHODS: In eight healthy participants, we measured venous flow volume and velocity in the femoral vein using duplex ultrasonography at 1, 10, 20, and 30 min after completing three modes of 1-min LEX exercises...
September 2017: Journal of Orthopaedic Surgery
https://www.readbyqxmd.com/read/29137116/altering-residue-134-confers-an-increased-substrate-range-of-alkylated-nucleosides-to-the-e-coli-ogt-protein
#19
Nadia M Schoonhoven, Derek K O'Flaherty, Francis P McManus, Lauralicia Sacre, Anne M Noronha, M Judith Kornblatt, Christopher J Wilds
O⁶-Alkylguanine-DNA alkyltransferases (AGTs) are proteins responsible for the removal of mutagenic alkyl adducts at the O⁶-atom of guanine and O⁴-atom of thymine. In the current study we set out to understand the role of the Ser134 residue in the Escherichia coli AGT variant OGT on substrate discrimination. The S134P mutation in OGT increased the ability of the protein to repair both O⁶-adducts of guanine and O⁴-adducts of thymine. However, the S134P variant was unable, like wild-type OGT, to repair an interstrand cross-link (ICL) bridging two O⁶-atoms of guanine in a DNA duplex...
November 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29136382/a-rhodium-cyanine-fluorescent-probe-detection-and-signaling-of-mismatches-in-dna
#20
Adela Nano, Adam N Boynton, Jacqueline K Barton
We report a bifunctional fluorescent probe that combines a rhodium metalloinsertor with a cyanine dye as the fluorescent reporter. The conjugate shows weak luminescence when free in solution or with well matched DNA but exhibits a significant luminescence increase in the presence of a 27-mer DNA duplex containing a central CC mismatch. DNA photocleavage experiments demonstrate that, upon photoactivation, the conjugate cleaves the DNA backbone specifically near the mismatch site on a 27-mer fragment, consistent with mismatch targeting...
November 14, 2017: Journal of the American Chemical Society
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