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https://www.readbyqxmd.com/read/29471537/the-c-terminal-helix-of-ribosomal-p-stalk-recognizes-a-hydrophobic-groove-of-elongation-factor-2-in-a-novel-fashion
#1
Takehito Tanzawa, Koji Kato, Dylan Girodat, Toyoyuki Ose, Yuki Kumakura, Hans-Joachim Wieden, Toshio Uchiumi, Isao Tanaka, Min Yao
Archaea and eukaryotes have ribosomal P stalks composed of anchor protein P0 and aP1 homodimers (archaea) or P1•P2 heterodimers (eukaryotes). These P stalks recruit translational GTPases to the GTPase-associated center in ribosomes to provide energy during translation. The C-terminus of the P stalk is known to selectively recognize GTPases. Here we investigated the interaction between the P stalk and elongation factor 2 by determining the structures of Pyrococcus horikoshii EF-2 (PhoEF-2) in the Apo-form, GDP-form, GMPPCP-form (GTP-form), and GMPPCP-form bound with 11 C-terminal residues of P1 (P1C11)...
February 20, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471520/effect-of-exposure-to-second-hand-smoke-from-husbands-on-biochemical-hyperandrogenism-metabolic-syndrome-and-conception-rates-in-women-with-polycystic-ovary-syndrome-undergoing-ovulation-induction
#2
Jian Li, Q Wu, Xiao-Ke Wu, Zhong-Ming Zhou, Ping Fu, Xiu-Hua Chen, Ying Yan, Xin Wang, Zheng-Wang Yang, Wei-Li Li, Elisabet Stener-Victorin, Richard S Legro, Ernest Hung-Yu Ng, Heping Zhang, Ben Willem J Mol, Chi Chiu Wang
STUDY QUESTION: Does second-hand smoke (SHS) exposure from husbands have adverse effects on sex hormones, metabolic profiles, clinical phenotypes and fertility outcomes in women with polycystic ovary syndrome (PCOS) undergoing ovulation induction? SUMMARY ANSWER: SHS exposure is associated with worsened biochemical hyperandrogenism, higher incidence of metabolic syndrome and reduced conception rates in women with PCOS. WHAT IS KNOWN ALREADY: Smoking in women impairs fecundity at some stages of the reproductive process including folliculogenesis, embryo transport, endometrial angiogenesis and uterine blood flow...
February 20, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29471506/a-translational-silencing-function-of-mcpip1-regnase-1-specified-by-the-target-site-context
#3
Gesine Behrens, Reinhard Winzen, Nina Rehage, Anneke Dörrie, Monika Barsch, Anne Hoffmann, Jörg Hackermüller, Christopher Tiedje, Vigo Heissmeyer, Helmut Holtmann
The expression of proteins during inflammatory and immune reactions is coordinated by post-transcriptional mechanisms. A particularly strong suppression of protein expression is exerted by a conserved translational silencing element (TSE) identified in the 3' UTR of NFKBIZ mRNA, which is among the targets of the RNA-binding proteins Roquin-1/2 and MCPIP1/Regnase-1. We present evidence that in the context of the TSE MCPIP1, so far known for its endonuclease activity toward mRNAs specified by distinct stem-loop (SL) structures, also suppresses translation...
February 19, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471489/the-h-subunit-of-the-restriction-endonuclease-cgli-contains-a-prototype-dead-z1-helicase-like-motor
#4
Paulius Toliusis, Giedre Tamulaitiene, Rokas Grigaitis, Donata Tuminauskaite, Arunas Silanskas, Elena Manakova, Ceslovas Venclovas, Mark D Szczelkun, Virginijus Siksnys, Mindaugas Zaremba
CglI is a restriction endonuclease from Corynebacterium glutamicum that forms a complex between: two R-subunits that have site specific-recognition and nuclease domains; and two H-subunits, with Superfamily 2 helicase-like DEAD domains, and uncharacterized Z1 and C-terminal domains. ATP hydrolysis by the H-subunits catalyses dsDNA translocation that is necessary for long-range movement along DNA that activates nuclease activity. Here, we provide biochemical and molecular modelling evidence that shows that Z1 has a fold distantly-related to RecA, and that the DEAD-Z1 domains together form an ATP binding interface and are the prototype of a previously undescribed monomeric helicase-like motor...
February 20, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471467/myeloperoxidase-influences-the-complement-regulatory-activity-of-complement-factor-h
#5
Su-Fang Chen, Feng-Mei Wang, Zhi-Ying Li, Feng Yu, Min Chen, Ming-Hui Zhao
Objective: The interaction between neutrophils and activation of alternative complement pathway plays a critical role in the pathogenesis of ANCA-associated vasculitis (AAV). MPO, which can be released from ANCA-stimulated neutrophils, was recently demonstrated to be capable of activating the alternative complement pathway. Here we aimed to investigate the interaction between MPO and factor H (FH), a key regulator of the alternative pathway, and its effect on the functional activities of FH...
February 19, 2018: Rheumatology
https://www.readbyqxmd.com/read/29471430/gwas-identifies-two-novel-colorectal-cancer-loci-at-16q24-1-and-20q13-12
#6
Chizu Tanikawa, Yoichiro Kamatani, Atsushi Takahashi, Yukihide Momozawa, Karine Leveque, Satoshi Nagayama, Koshi Mimori, Masaki Mori, Hideshi Ishii, Johji Inazawa, Jun Yasuda, Akito Tsuboi, Atsushi Shimizu, Makoto Sasaki, Taiki Yamaji, Norie Sawada, Motoki Iwasaki, Shoichiro Tsugane, Mariko Naito, Kenji Wakai, Teruhide Koyama, Toshiro Takezaki, Koichiro Yuji, Yoshinori Murakami, Yusuke Nakamura, Michiaki Kubo, Koichi Matsuda
Colorectal cancer is the fourth leading cause of cancer mortality worldwide. Genome-wide association studies (GWAS) identified more than 50 colorectal cancer loci. However, most of the previous studies were conducted in European populations, and host genetic factors among Japanese population are largely remained to be identified. To identify novel loci in the Japanese population, here, we performed a large-scale GWAS using 6,692 cases and 27,178 controls followed by a replication analysis using more than 11,000 case-control samples...
February 17, 2018: Carcinogenesis
https://www.readbyqxmd.com/read/29471425/foxl2c134w-induced-cyp19-expression-via-cooperation-with-smad3-in-hgrc1-cells
#7
Martina Belli, Nahoko Iwata, Tomoko Nakamura, Akira Iwase, Dwayne Stupack, Shunichi Shimasaki
Germline knockout studies in female mice demonstrated an essential role for FOXL2 in early follicle development, while an inducible granulosa cell (GC) specific deletion of Foxl2 in adults has shown ovary-to-testis somatic sex reprogramming. In women, over 120 different germline mutations in the FOXL2 gene have been shown to cause blepharophimosis/ptosis/epicantus inversus syndrome associated with or without primary ovarian insufficiency. By contrast, a single somatic mutation (FOXL2C134W) accounts for almost all adult-type GC tumors (aGCTs)...
February 19, 2018: Endocrinology
https://www.readbyqxmd.com/read/29471413/combined-cistrome-and-transcriptome-analysis-of-ski-in-aml-cells-identifies-ski-as-a-co-repressor-for-runx1
#8
Christine Feld, Peeyush Sahu, Miriam Frech, Florian Finkernagel, Andrea Nist, Thorsten Stiewe, Uta-Maria Bauer, Andreas Neubauer
SKI is a transcriptional co-regulator and overexpressed in various human tumors, for example in acute myeloid leukemia (AML). SKI contributes to the origin and maintenance of the leukemic phenotype. Here, we use ChIP-seq and RNA-seq analysis to identify the epigenetic alterations induced by SKI overexpression in AML cells. We show that approximately two thirds of differentially expressed genes are up-regulated upon SKI deletion, of which >40% harbor SKI binding sites in their proximity, primarily in enhancer regions...
February 20, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471396/involvement-of-epigenetic-modifications-of-gabaergic-interneurons-in-basolateral-amygdala-in-anxiety-like-phenotype-of-prenatally-stressed-mice
#9
Chunting Zhu, Min Liang, Yingchun Li, Xuejiao Feng, Juan Hong, Rong Zhou
Background: Prenatal stress is considered a risk factor for anxiety disorder. Downregulation in the expression of GABAergic gene, that is, glutamic acid decarboxylase 67, associated with DNA methyltransferase overexpression in GABAergic neurons has been regarded as a characteristic component of anxiety disorder. Prenatal stress has an adverse effect on the development of the basolateral amygdala, which is a key region in anxiety regulation. The aim of this study is to analyze the possibility of epigenetic alterations of GABAergic neurons in the basolateral amygdala participating in prenatal stress-induced anxiety...
February 20, 2018: International Journal of Neuropsychopharmacology
https://www.readbyqxmd.com/read/29471365/integrator-subunit-4-is-a-symplekin-like-scaffold-that-associates-with-ints9-11-to-form-the-integrator-cleavage-module
#10
Todd R Albrecht, Sergey P Shevtsov, Yixuan Wu, Lauren G Mascibroda, Natoya J Peart, Kai-Lieh Huang, Iain A Sawyer, Liang Tong, Miroslav Dundr, Eric J Wagner
Integrator (INT) is a transcriptional regulatory complex associated with RNA polymerase II that is required for the 3'-end processing of both UsnRNAs and enhancer RNAs. Integrator subunits 9 (INTS9) and INTS11 constitute the catalytic core of INT and are paralogues of the cleavage and polyadenylation specificity factors CPSF100 and CPSF73. While CPSF73/100 are known to associate with a third protein called Symplekin, there is no paralog of Symplekin within INT raising the question of how INTS9/11 associate with the other INT subunits...
February 20, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471350/structural-and-mechanistic-insights-into-uhrf1-mediated-dnmt1-activation-in-the-maintenance-dna-methylation
#11
Tao Li, Linsheng Wang, Yongming Du, Si Xie, Xi Yang, Fuming Lian, Zhongjun Zhou, Chengmin Qian
UHRF1 plays multiple roles in regulating DNMT1-mediated DNA methylation maintenance during DNA replication. The UHRF1 C-terminal RING finger functions as an ubiquitin E3 ligase to establish histone H3 ubiquitination at Lys18 and/or Lys23, which is subsequently recognized by DNMT1 to promote its localization onto replication foci. Here, we present the crystal structure of DNMT1 RFTS domain in complex with ubiquitin and highlight a unique ubiquitin binding mode for the RFTS domain. We provide evidence that UHRF1 N-terminal ubiquitin-like domain (UBL) also binds directly to DNMT1...
February 19, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29471349/intestinal-colonization-traits-of-pandemic-multidrug-resistant-escherichia-coli-st131
#12
Sohinee Sarkar, Melanie L Hutton, Dimitrios Vagenas, Rinaldo Ruter, Stephanie Schüller, Dena Lyras, Mark A Schembri, Makrina Totsika
Background: Epidemiological studies point to the gut as a key reservoir of multidrug resistant Escherichia coli multilocus sequence type 131 (ST131), a globally dominant pathogenic clone causing urinary tract and bloodstream infections. Here we report a detailed investigation of its intestinal lifestyle. Methods: Clinical ST131 isolates and type 1 fimbriae null mutants were assessed for colonization of human intestinal epithelia and in mouse intestinal colonization models...
February 16, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29471325/dual-stain-with-satb2-and-ck20-villin-is-useful-to-distinguish-colorectal-carcinomas-from-other-tumors
#13
Zaibo Li, Jonathan B Rock, Rachel Roth, Amy Lehman, William L Marsh, Adrian Suarez, Wendy L Frankel
Objectives: Small sample size limits the number of immunostains that may be attempted in colorectal carcinoma (CRC) biopsy specimens. We investigated the utility of dual stain with special AT-rich sequence binding protein 2 (SATB2) or caudal-type homeobox 2 (CDX2) and cytokeratin 20 (CK20) or villin in identifying CRC. Methods: Tissue microarrays with 222 CRCs and 375 other carcinomas were built. Dual stain was performed pairing nuclear stains CDX2 or SATB2 with CK20 or villin...
February 17, 2018: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29471289/single-nucleotide-polymorphism-facilitated-down-regulation-of-the-cohesin-stromal-antigen-1-implications-for-colorectal-cancer-racial-disparities
#14
Somenath Datta, Richard M Sherva, Mart De La Cruz, Michelle T Long, Priya Roy, Vadim Backman, Sanjib Chowdhury, Hemant K Roy
The biological underpinnings for racial disparities in colorectal cancer (CRC) incidence remain to be elucidated. We have previously reported that the cohesin SA-1 down-regulation is an early event in colon carcinogenesis which is dramatically accentuated in African-Americans. In order to investigate the mechanism, we evaluated single nucleotide polymorphisms (SNPs) for association with SA-1-related outcomes followed by gene editing of candidate SNP. We observed that rs34149860 SNP was significantly associated with a lower colonic mucosal SA-1 expression and evaluation of public databases showed striking racial discordance...
February 19, 2018: Neoplasia: An International Journal for Oncology Research
https://www.readbyqxmd.com/read/29471281/theoretical-and-experimental-study-for-the-biomimetic-recognition-of-levothyroxine-hormone-on-magnetic-molecularly-imprinted-polymer
#15
Silio Lima Moura, Laura Martinez Fajardo, Leonardo Dos Anjos Cunha, Maria Del Pilar Taboada Sotomayor, Francisco Bolivar Correto Machado, Luiz Fernando Araújo Ferrão, Maria Isabel Pividori
This study addresses the rational design of a magnetic molecularly imprinted polymer (magnetic-MIP) for the selective recognition of the hormone levothyroxine. The theoretical study was carried out by the density functional theory (DFT) computations considering dispersion interaction energies, and using the D2 Grimme's correction. The B97-D/def2-SV(P)/PCM method is used not only for studying the structure of the template the and monomer-monomer interactions, but also to assess the stoichiometry, noncovalent binding energies, solvation effects and thermodynamics properties such as binding energy...
January 31, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29471264/collective-transcriptomic-deregulation-of-hypertrophic-and-dilated-cardiomyopathy-importance-of-fibrotic-mechanism-in-heart-failure
#16
Beutline Malgija, Nachimuthu Senthil Kumar, Shanmughavel Piramanayagam
Myocardial fibrosis reside a common pathological feature in hypertrophic and dilated cardiomyopathy that results in ventricular dysfunction leading to heart failure. Though several studies reported the role of fibrosis in cardiac diseases, their pathologic mechanisms leading to heart failure remains unclear. A few studies have proposed integrated analysis of microarray information and protein-protein interaction (PPI) systems to discover subnetwork markers related to diagnosis and prognosis of the disease. In addition to PPI networks, we incorporated miRNAs and transcription factors to find the putative miRNAs and transcription factors that might regulate the pathological process and progression of cardiomyopathy and their further progression to heart failure...
February 10, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29471249/post-mortem-biochemistry-of-nse-and-s100b-a-supplemental-tool-for-detecting-a-lethal-traumatic-brain-injury
#17
Monique Sieber, Jan Dreßler, Heike Franke, Dirk Pohlers, Benjamin Ondruschka
PURPOSE: Traumatic brain injury (TBI) is a very common entity that leads to numerous fatalities all over the world. Therefore, forensic pathologists are in desperate need of supplemental methodological tools for the diagnosis of TBI in everyday practice besides the standard autopsy. The present study determined post-mortem neuron specific enolase (NSE) and S100 calcium-binding protein B (S100B) levels as biological markers of an underlying TBI in autopsy cases. METHODS: Paired serum and CSF samples of 92 fatalities were collected throughout routine autopsies...
February 14, 2018: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/29471243/trps1-suppresses-breast-cancer-epithelial-mesenchymal-transition-program-as-a-negative-regulator-of-suz12
#18
Jing Hu, Peng Su, Meng Jiao, Xinnuo Bai, Mei Qi, Hui Liu, Zhen Wu, Jingtian Sun, Gengyin Zhou, Bo Han
Breast cancer (BC) is among the most common malignant diseases and metastasis is the handcuff of treatment. Cancer metastasis is a multistep process associated with the epithelial-mesenchymal transition (EMT) program. Several studies have demonstrated that transcriptional repressor GATA binding 1 (TRPS1) played important roles in development and progression of primary BC. In this study we sought to identify the mechanisms responsible for this function of TRPS1 in the continuum of the metastatic cascade. Here we described that TRPS1 was significantly associated with BC metastasis using public assessable datasets...
February 19, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29471235/competition-between-heavy-metal-ions-for-binding-sites-in-lichens-implications-for-biomonitoring-studies
#19
Luca Paoli, Andrea Vannini, Fabrizio Monaci, Stefano Loppi
The competitive behavior of divalent heavy metals (Cd, Cr, Pb, Zn) during cation uptake was investigated in the foliose lichen Xanthoria parietina. Lichen thalli were incubated with solutions containing 10 and 100 μM of CdCl2, CuCl2, and ZnCl2 as well as 5 and 50 μM of Pb(NO3 )2 , tested individually and in combination (Cd2+ +Cu2+ +Pb2+ +Zn2+ ). The analysis of molar concentrations suggests that a competition between cations for binding sites in X. parietina does exist. The decrease in net uptake between single and mixed solutions ranged between 14 and 29% at the lowest concentration and between 38 and 68% at the highest concentration...
February 19, 2018: Chemosphere
https://www.readbyqxmd.com/read/29471169/pegylated-dendritic-polyglycerol-conjugate-targeting-ncam-expressing-neuroblastoma-limitations-and-challenges
#20
Laura Isabel Vossen, Ela Markovsky, Anat Eldar-Boock, Harald Rune Tschiche, Stefanie Wedepohl, Evgeny Pisarevsky, Ronit Satchi-Fainaro, Marcelo Calderón
Neural cell adhesion molecule (NCAM) is found to be a stem-cell marker in several tumor types and its overexpression is known to correlate with increased metastatic capacity. To combine extravasation- and ligand-dependent targeting to NCAM overexpressing-cells in the tumor microenvironment, we developed a PEGylated NCAM-targeted dendritic polyglycerol (PG) conjugate. Here, we describe the synthesis, physico-chemical characterization and biological evaluation of a PG conjugate bearing the mitotic inhibitor paclitaxel (PTX) and an NCAM-targeting peptide (NTP)...
February 19, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
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