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https://www.readbyqxmd.com/read/28813513/validation-of-commercial-mas-receptor-antibodies-for-utilization-in-western-blotting-immunofluorescence-and-immunohistochemistry-studies
#1
Valeria Burghi, Natalia Cristina Fernández, Yamila Belén Gándola, Verónica Gabriela Piazza, Diego Tomás Quiroga, Érica Guilhen Mario, Janaína Felix Braga, Michael Bader, Robson Augusto Souza Santos, Fernando Pablo Dominici, Marina Cecilia Muñoz
Mas receptor (MasR) is a G protein-coupled receptor proposed as a candidate for mediating the angiotensin (Ang)-converting enzyme 2-Ang (1-7) protective axis of renin-angiotensin system. Because the role of this receptor is not definitively clarified, determination of MasR tissue distribution and expression levels constitutes a critical knowledge to fully understanding its function. Commercially available antibodies have been widely employed for MasR protein localization and quantification, but they have not been adequately validated...
2017: PloS One
https://www.readbyqxmd.com/read/28813091/guillain-barr%C3%A3-syndrome-celebrating-a-century
#2
Marcos R G de Freitas, Cecília M Vidal, Marco Orsini
A hundred years ago, Guillain, Barré and Strohl described a syndrome with a predominant motor acute or subacute polyneuritis, albumin-cytologic dissociation in the cerebrospinal fluid, and a benign course. Before them, many other authors, such as Landry, Duménil, Osler, and Grainger Stewart had described similar cases although they had not performed lumbar punctures. In this work, we outline certain features of the beginning of this famous syndrome.
August 2017: Arquivos de Neuro-psiquiatria
https://www.readbyqxmd.com/read/28812997/distribution-of-fmr1-and-fmr2-repeats-in-argentinean-patients-with-primary-ovarian-insufficiency
#3
Lucía Daniela Espeche, Violeta Chiauzzi, Ianina Ferder, Mehrnoosh Arrar, Andrea Paula Solari, Carlos David Bruque, Marisol Delea, Susana Belli, Cecilia Soledad Fernández, Noemí Delia Buzzalino, Eduardo Hernán Charreau, Liliana Beatriz Dain
The premutation state of FMR1 (Fragile X Mental Retardation 1) has been associated with primary ovarian insufficiency (POI), and is the most common known genetic cause for 46,XX patients. Nevertheless, very few studies have analyzed its frequency in Latin American populations. Additionally, a relationship between alleles carrying a cryptic microdeletion in the 5'UTR of FMR2 and the onset of POI has only been studied in one population. Our aim was to analyze the incidence of FMR1 premutations and putative microdeletions in exon 1 of FMR2 in a cohort of Argentinean women with POI...
August 16, 2017: Genes
https://www.readbyqxmd.com/read/28811977/epigenetic-regulation-of-the-extrinsic-oncosuppressor-ptx3-gene-in-inflammation-and-cancer
#4
Marcello Rubino, Paolo Kunderfranco, Gianluca Basso, Carolina Magdalen Greco, Fabio Pasqualini, Simone Serio, Massimo Roncalli, Luigi Laghi, Alberto Mantovani, Roberto Papait, Cecilia Garlanda
PTX3 is a component of the humoral arm of innate immunity and an extrinsic oncosuppressor gene taming tumor-promoting inflammation. Here, we show that two enhancers differently regulate PTX3 expression: enhancer 1, located 230 kb upstream of PTX3 promoter, mediated the action of inflammatory transcription factors; and enhancer 2, encompassing PTX3 second exon, was implicated in pre-initiation complex assembly. Polycomb repressive complex 2 silenced these regulatory elements and the promoter in basal condition...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28811681/osteopontin-impacts-west-nile-virus-pathogenesis-and-resistance-by-regulating-inflammasome-components-and-cell-death-in-the-central-nervous-system-at-early-time-points
#5
Nikki Bortell, Claudia Flynn, Bruno Conti, Howard S Fox, Maria Cecilia G Marcondes
Osteopontin (OPN) is a molecule that is common in central nervous system (CNS) pathologies, which participates in the activation, migration, and survival of inflammatory cells. However, the mechanisms by which OPN modulates inflammatory pathways are not clear. To understand the role of OPN in CNS viral infections, we used a lethal mouse model of West Nile virus (WNV), characterized by the injection of high doses of the Eg101 strain of WNV, causing the increase of OPN levels in the brain since early time points...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28808842/colonic-motor-dysfunctions-in-a-mouse-model-of-high-fat-diet-induced-obesity-an-involvement-of-a2b-adenosine-receptors
#6
Luca Antonioli, Carolina Pellegrini, Matteo Fornai, Erika Tirotta, Daniela Gentile, Laura Benvenuti, Maria Cecilia Giron, Valentina Caputi, Ilaria Marsilio, Genny Orso, Nunzia Bernardini, Cristina Segnani, Chiara Ippolito, Balázs Csóka, Zoltán H Németh, György Haskó, Carmelo Scarpignato, Corrado Blandizzi, Rocchina Colucci
Adenosine A2B receptors (A2BR) regulate several enteric functions. However, their implication in the pathophysiology of intestinal dysmotility associated with high-fat diet (HFD)-induced obesity has not been elucidated. We investigated the expression of A2BR in mouse colon and their role in the mechanisms underlying the development of enteric dysmotility associated with obesity. Wild-type C57BL/6J mice were fed with HFD (60% kcal from fat) or normocaloric diet (NCD; 18% kcal from fat) for 8 weeks. Colonic A2BR localization was examined by immunofluorescence...
August 14, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28808549/characterization-of-natural-variation-in-north-american-atlantic-salmon-populations-salmonidae-salmo-salar-at-a-locus-with-a-major-effect-on-sea-age
#7
Henrik Kusche, Guillaume Côté, Cécilia Hernandez, Eric Normandeau, Damien Boivin-Delisle, Louis Bernatchez
Age at maturity is a key life-history trait of most organisms. In anadromous salmonid fishes such as Atlantic Salmon (Salmo salar), age at sexual maturity is associated with sea age, the number of years spent at sea before the spawning migration. For the first time, we investigated the presence of two nonsynonymous vgll3 polymorphisms in North American Atlantic Salmon populations that relate to sea age in European salmon and quantified the natural variation at these and two additional candidate SNPs from two other genes...
August 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28807597/reference-values-of-intrinsic-muscle-strength-of-the-hand-of-adolescents-and-young-adults
#8
Chao-Ying Chen, Corey W McGee, Tonya L Rich, Cecília N Prudente, Bernadette T Gillick
STUDY DESIGN: A cross-sectional clinical measurement study. INTRODUCTION: Measuring intrinsic hand muscle strength helps evaluate hand function or therapeutic outcomes. However, there are no established normative values in adolescents and young adults between 13 and 20 years of age. PURPOSE OF THE STUDY: To measure hand intrinsic muscle strength and identify associated factors that may influence such in adolescents and young adults through use of the Rotterdam intrinsic hand myometer...
August 11, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
https://www.readbyqxmd.com/read/28806420/perceptions-of-health-risks-of-cigarette-smoking-a-new-measure-reveals-widespread-misunderstanding
#9
Jon A Krosnick, Neil Malhotra, Cecilia Hyunjung Mo, Eduardo F Bruera, LinChiat Chang, Josh Pasek, Randall K Thomas
Most Americans recognize that smoking causes serious diseases, yet many Americans continue to smoke. One possible explanation for this paradox is that perhaps Americans do not accurately perceive the extent to which smoking increases the probability of adverse health outcomes. This paper examines the accuracy of Americans' perceptions of the absolute risk, attributable risk, and relative risk of lung cancer, and assesses which of these beliefs drive Americans' smoking behavior. Using data from three national surveys, statistical analyses were performed by comparing means, medians, and distributions, and by employing Generalized Additive Models...
2017: PloS One
https://www.readbyqxmd.com/read/28806334/cochlear-implantation-in-postlingual-adult-patients-with-long-term-auditory-deprivation
#10
Maria Del Mar Medina, Ruben Polo, Auxiliadora Gutierrez, Alfonso Muriel, Miguel Vaca, Cecilia Perez, Adela Cordero, Ignacio Cobeta
OBJECTIVE: The role of long-term hearing deprivation in cochlear implant performance is not well established, and the limits for implanting an auditory deprived ear are still unknown. The objective of this study was to evaluate the effect of long-term auditory deprivation time on cochlear implant results. STUDY DESIGN: Retrospective case review study. SETTING: Cochlear implantation tertiary referral center. PATIENTS: Adult patients with postlingual deafness...
September 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28806121/social-capital-accumulation-in-location-based-mobile-game-playing-a-multiple-process-approach
#11
Hei-Tung Sung, Leif Sigerson, Cecilia Cheng
In the summer of 2016, the widespread popularization of the game Pokémon Go brought the public's attention to the possible socialization benefits associated with location-based mobile games (LBMGs), yet no studies to date have investigated this issue. LBMGs deserve greater research attention because this type of game provides a unique gaming experience that encourages players to leave their homes and navigate around their neighborhood. This distinctive feature may promote more face-to-face interactions among LBMG players...
August 2017: Cyberpsychology, Behavior and Social Networking
https://www.readbyqxmd.com/read/28805276/visible-light-mediated-selective-arylation-of-cysteine-in-batch-and-flow
#12
Cecilia Bottecchia, Maarten Rubens, Smita Gunnoo, Volker Hessel, Annemieke Madder, Timothy Noel
A mild visible light-mediated strategy for cysteine arylation is presented. The method relies on the use of Eosin Y as a metal-free photocatalyst and aryldiazonium salts as arylating agents. The reaction can be significantly accelerated in a microflow reactor, whilst allowing the in situ formation of the required diazonium salts. The batch and flow protocol described herein can be applied to obtain a broad series of arylated cysteine derivatives and arylated cysteine-containing dipeptides. Moreover, the method was applied to the chemoselective arylation of a model peptide in biocompatible reaction conditions (room temperature, PBS buffer) within a short reaction time...
August 14, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28804960/handle-with-care-rethinking-the-rights-versus-culture-dichotomy-in-cancer-disclosure-in-india
#13
Cecilia Coale Van Hollen
Medical practitioners, bioethicists, psychologists, and anthropologists have debated whether it is ethical to disclose or withhold information from patients about cancer diagnoses. This debate is framed as pitting universal individual human rights against cultural pluralism. The rights-based argument asserts that people have a right to information about their own health to make the best decisions about their treatment. The cultural variation argument suggests that in some cultural contexts there is a perception that information about one's cancer diagnosis may cause more harm than good due to the psychological trauma this may cause...
August 13, 2017: Medical Anthropology Quarterly
https://www.readbyqxmd.com/read/28804505/an-etiologic-prediction-model-incorporating-biomarkers-to-predict-the-bladder-cancer-risk-associated-with-occupational-exposure-to-aromatic-amines-a-pilot-study
#14
Giuseppe Mastrangelo, Angela Carta, Cecilia Arici, Sofia Pavanello, Stefano Porru
BACKGROUND: No etiological prediction model incorporating biomarkers is available to predict bladder cancer risk associated with occupational exposure to aromatic amines. METHODS: Cases were 199 bladder cancer patients. Clinical, laboratory and genetic data were predictors in logistic regression models (full and short) in which the dependent variable was 1 for 15 patients with aromatic amines related bladder cancer and 0 otherwise. The receiver operating characteristics approach was adopted; the area under the curve was used to evaluate discriminatory ability of models...
2017: Journal of Occupational Medicine and Toxicology
https://www.readbyqxmd.com/read/28804480/microbial-community-structure-and-the-persistence-of-cyanobacterial-populations-in-salt-crusts-of-the-hyperarid-atacama-desert-from-genome-resolved-metagenomics
#15
Kari M Finstad, Alexander J Probst, Brian C Thomas, Gary L Andersen, Cecilia Demergasso, Alex Echeverría, Ronald G Amundson, Jillian F Banfield
Although once thought to be devoid of biology, recent studies have identified salt deposits as oases for life in the hyperarid Atacama Desert. To examine spatial patterns of microbial species and key nutrient sources, we genomically characterized 26 salt crusts from three sites along a fog gradient. The communities are dominated by a large variety of Halobacteriales and Bacteroidetes, plus a few algal and Cyanobacterial species. CRISPR locus analysis suggests the distribution of a single Cyanobacterial population among all sites...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28803634/arteria-lusoria-a-rare-cause-of-tracheal-compression
#16
Ana Nóbrega-Pinto, Isabel Carvalho, Cecília Almeida-Sousa
No abstract text is available yet for this article.
August 10, 2017: Acta Otorrinolaringológica Española
https://www.readbyqxmd.com/read/28803377/temporary-successful-results-of-ventral-rectopexy-for-enterocele-surgical-correction-about-138-patients
#17
Diane Mege, Arnaud Sans, Aurélie Maignan, Julie Duclos, Cécilia Frasconi, Remy Le Huu Nho, Nicolas Pirro, Igor Sielezneff
PURPOSE: This work aimed to analyse short- and long-term results of enterocele surgical treatment by ventral rectopexy. METHODS: All females who underwent ventral rectopexy for enterocele in our department were included. All patients underwent standardized preoperative evaluation. Data was retrospectively collected, after examination of patients or by telephone survey. Postoperative evaluation was performed by an independent observer. RESULTS: One hundred thirty-eight females (median age = 63 years [21-86 years]) were included...
August 12, 2017: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/28803133/predictive-value-of-multiple-cytokines-and-chemokines-for-mortality-in-an-admixed-population-15-year-follow-up-of-the-bambui-epigen-brazil-cohort-study-of-aging
#18
Maria Fernanda Lima-Costa, Juliana Vaz de Melo Mambrini, Karen Cecília de Lima Torres, Sérgio Viana Peixoto, Cesar de Oliveira, Eduardo Tarazona-Santos, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho
Inflammation, particularly elevated IL-6 serum levels, has been associated with increased mortality risk, mostly in Caucasians. The influence of genetic ethno-racial background on this association is unknown. We examined associations between baseline serum levels of Interleukin-6 (IL-6) and other cytokines (IL1-2, TNF, IL-10, and IL1β) and chemokines (CCL2, CCL5, CXCL8, CXCL9 and CXCL10) with 15-year mortality in 1,191 admixed Brazilians aged 60years and over. Elevated IL6 level (but not other biomarkers) was associated with increased risk of deaths with fully adjusted hazard ratios of 1...
August 9, 2017: Experimental Gerontology
https://www.readbyqxmd.com/read/28802954/creation-of-a-neovagina-by-laparoscopic-modified-davydov-vaginoplasty-in-patients-with-partial-androgen-insensitivity-syndrome
#19
Stefano Bianchi, Nicola Berlanda, Federica Brunetti, Alessandro Bulfoni, Cecilia Ferrero Caroggio, Luigi Fedele
OBJECTIVE: To evaluate the feasibility, safety and outcome of the laparoscopic modified Davydov vaginoplasty in subjects with partial androgen insensitivity syndrome (PAIS). DESIGN: Retrospective cohort study. CANADIAN TASK FORCE CLASSIFICATION OF STUDY DESIGN: III. SETTING: Tertiary referral Center. PATIENTS: Ten continuous patients operated between October 2008 and May 2014. INTERVENTIONS: Laparoscopic modified Davydov vaginoplasty...
August 9, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28802709/sex-differences-in-cancer-risk-and-survival-a-swedish-cohort-study
#20
Cecilia Radkiewicz, Anna L V Johansson, Paul W Dickman, Mats Lambe, Gustaf Edgren
AIM: The aim of this study is to firmly delineate temporal and age trends regarding sex discrepancies in cancer risk and survival as well as quantifying the potential gain achieved by eliminating this inequality. METHODS: We performed a population-based cohort study using data on all adult incident cancer cases (n = 872,397) recorded in the Swedish Cancer Register in 1970-2014. To assess the associations between sex and cancer risk and sex and survival, male-to-female incidence rate ratios (IRRs) and excess mortality ratios (EMRs) adjusted for age and year of diagnosis were estimated using Poisson regression...
August 10, 2017: European Journal of Cancer
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