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https://www.readbyqxmd.com/read/28938608/use-of-confocal-laser-endomicroscopy-with-a-fluorescently-labeled-fatty-acid-to-diagnose-colorectal-neoplasms
#1
Feihong Deng, Yuan Fang, Zhiyong Shen, Wei Gong, Tao Liu, Jing Wen, Wanling Zhang, Xianjun Zhu, Hui Zhong, Tong Wang, Fachao Zhi, Biao Nie
Endoscopic treatment for early colorectal cancer closely correlates with patient prognosis. However, endoscopic differentiation between carcinomas and non-neoplastic lesions remains difficult. Here, we topically stained colorectal neoplasms with a fatty acid analogue (BODIPY-FA) and quantified the fluorescent signals using confocal laser endomicroscopy (CLE) and fluorescence microscopy. We also analyzed protein expression in colorectal cancer tissues. We found that expression of fatty acid synthase was elevated, while the expression of fatty acid transporters was reduced in colorectal cancer...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28937893/effect-of-enamel-deproteinization-in-primary-teeth
#2
Ananthi Christopher, R Krishnakumar, N Venugopal Reddy, G Rohini
OBJECTIVE: To evaluate and compare the topographical features of enamel surface, etched with different materials. STUDY DESIGN: 10 extracted human primary molars were randomly selected and cut and trimmed to 1 mm(2). Each group comprised of 10 blocks and the enamel was treated as follows: Group I-35% H3PO4; Group II-5.25% NaOCl + 35% H3PO4 ;Group III-5.25% NaOCl; Group IV no treatment was carried out. All the samples were prepared for Scanning electron microscope analysis...
September 22, 2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28937579/pyrethroids-inhibit-k2p-channels-and-activate-sensory-neurons-basis-of-insecticide-induced-paraesthesias
#3
Aida Castellanos, Alba Andres, Laura Bernal, Gerard Callejo, Nuria Comes, Arcadi Gual, Jonathan P Giblin, Carolina Roza, Xavier Gasull
Pyrethroid insecticides are widely used for pest control, in agriculture or in human public health commonly as a topical treatment for scabies and head lice. Exposure to pyrethroids such as permethrin or tetramethrin (TM) causes sensory alterations such as transient pain, burning, stinging sensations and paraesthesias. Despite the well-known effects of pyrethroids on sodium channels, actions on other channels that control sensory neuron excitability are less studied. Given the role of two-pore domain potassium (K2P) channels in modulating sensory neuron excitability and firing, both in physiological and pathological conditions, we examined the effect of pyrethroids on K2P channels mainly expressed in sensory neurons...
September 19, 2017: Pain
https://www.readbyqxmd.com/read/28934971/assessment-classification-and-treatment-of-calcinosis-as-a-complication-of-juvenile-dermatomyositis-a-survey-of-pediatric-rheumatologists-by-the-childhood-arthritis-and-rheumatology-research-alliance-carra
#4
A B Orandi, K W Baszis, V R Dharnidharka, A M Huber, M F Hoeltzel
BACKGROUND: There is no standardized approach to the management of JDM-associated calcinosis and its phenotypes. Current knowledge of treatment outcomes is confined to small series and case reports. We describe physician perspectives toward diagnostic approach, classification and treatment directly targeting calcinosis, independent of overall JDM therapy. METHODS: An electronic survey of 22 questions was organized into sections regarding individual practices of assessment, classification and treatment of calcinosis, including perceived successes of therapies...
September 21, 2017: Pediatric Rheumatology Online Journal
https://www.readbyqxmd.com/read/28928779/evaluation-of-the-effect-of-three-mouthwashes-on-the-mechanical-properties-and-surface-morphology-of-several-orthodontic-wires-an-in-vitro-study
#5
Hossein Aghili, Sogra Yassaei, Farzaneh Eslami
BACKGROUND: The aim of this study was to evaluate and compare the changes in the mechanical properties and surface morphology of different orthodontic wires after immersion in three mouthwash solutions. MATERIALS AND METHODS: In this in vitro study, five specimens of each of 0.016 inch nickel titanium (NiTi), coated NiTi, and stainless steel orthodontic wires were selected. The specimens were immersed in 0.05% sodium fluoride (NaF), 0.2% chlorhexidine, Zataria multiflora extract, and distilled water (control) for 1...
July 2017: Dental Research Journal
https://www.readbyqxmd.com/read/28926823/preparation-characterization-and-application-of-urease-nanoparticles-for-construction-of-an-improved-potentiometric-urea-biosensor
#6
Seema Jakhar, C S Pundir
The nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine dihydrochloride. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM), UV and Fourier transform infrared (FTIR) spectroscopy. The TEM images of urease NPs showed their size in the range, 18-100nm with an average of 51.2nm. The ENPs were more active and stable with a longer shelf life than native enzyme molecules...
September 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28924482/atypical-presentation-of-central-pontine-myelinolysis-in-hyperglycemia
#7
Swapna Talluri, Raghu Charumathi, Muhammad Khan, Kerri Kissell
Central pontine myelinolysis (CPM) usually occurs with rapid correction of severe chronic hyponatremia. Despite the pronounced fluctuations in serum osmolality, CPM is rarely seen in diabetics. This is a case report of CPM associated with hyperglycemia. A 45-year-old non-smoking and non-alcoholic African American male with past medical history of type 2 diabetes, hypertension, stage V chronic kidney disease and hypothyroidism presented with a two-week history of intermittent episodes of gait imbalance, slurred speech and inappropriate laughter...
2017: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/28922372/reduction-of-organelle-motility-by-removal-of-potassium-and-other-solutes
#8
John W Murray, David Yin, Allan W Wolkoff
There are surprisingly few studies that describe how the composition of cell culture medium may affect the trafficking of organelles. Here we utilize time lapse multi-channel fluorescent imaging to show that short term exposure of Huh-7 cells to medium lacking potassium, sodium, or chloride strongly reduces but does not eliminate the characteristic back and forth and cell-traversing movement of fluorescent EGF (FL-EGF) containing organelles. We focused on potassium because of its relatively low abundance in media and serum and its energy requiring accumulation into cells...
2017: PloS One
https://www.readbyqxmd.com/read/28920862/enhanced-endothelium-epithelial-sodium-channel-signaling-prompts-left-ventricular-diastolic-dysfunction-in-obese-female-mice
#9
Guanghong Jia, Javad Habibi, Annayya R Aroor, Michael A Hill, Vincent G DeMarco, Li E Lee, Lixin Ma, Brady J Barron, Adam Whaley-Connell, James R Sowers
OBJECTIVE: Enhanced activation of cell specific mineralocorticoid receptors (MRs) in obesity plays a key role in the development of cardiovascular disease including cardiac diastolic dysfunction as a critical prognosticator. Our previous investigations demonstrated that selective endothelium MR activation promotes a maladaptive inflammatory response and fibrosis in cardiovascular tissue in female mice fed a western diet (WD), and this was associated with expression and activation of the epithelial sodium channel on the surface of endothelial cells (EnNaC)...
September 8, 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28920628/fluoroquinolones-as-imaging-agents-for-bacterial-infection
#10
Syed Ali Raza Naqvi, Karl Drlica
Diagnosis of deep-seated bacterial infection is difficult, as neither standard anatomical imaging nor radiolabeled, autologous leukocytes distinguish sterile inflammation from infection. Two recent imaging efforts are receiving attention: (1) radioactive derivatives of sorbitol show good specificity with Gram-negative bacterial infections, and (2) success in combining anatomical and functional imaging for cancer diagnosis has rekindled interest in (99m)Tc-fluoroquinolone-based imaging. With the latter, computed tomography (CT) would be combined with single-photon-emission-computed tomography (SPECT) to detect (99m)Tc-fluoroquinolone-bacterial interactions...
September 18, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28917285/fluorescence-imaging-agents-in-tumor-resection
#11
REVIEW
Walter Stummer, Eric Suero Molina
Intraoperative fluorescence imaging allows real-time identification of diseased tissue during surgery without being influenced by brain shift and surgery interruption. 5-Aminolevulinic acid, useful for malignant gliomas and other tumors, is the most broadly explored compound approved for fluorescence-guided resection. Intravenous fluorescein sodium has recently received attention, highlighting tumor tissue based on extravasation at the blood-brain barrier (defective in many brain tumors). Fluorescein in perfused brain, unselective extravasation in brain perturbed by surgery, and propagation with edema are concerns...
October 2017: Neurosurgery Clinics of North America
https://www.readbyqxmd.com/read/28914408/3d-volumetry-and-its-correlation-between-postoperative-gastric-volume-and-excess-weight-loss-after-sleeve-gastrectomy
#12
Andrés Hanssen, Sergio Plotnikov, Geylor Acosta, José Tomas Nuñez, José Haddad, Carmen Rodriguez, Claudia Petrucci, Diego Hanssen, Rafael Hanssen
BACKGROUND: The volume of the postoperative gastric remnant is a key factor in excess weight loss (EWL) after sleeve gastrectomy (SG). Traditional methods to estimate gastric volume (GV) after bariatric procedures are often inaccurate; usually conventional biplanar contrast studies are used. METHODS: Thirty patients who underwent SG were followed prospectively and evaluated at 6 months after the surgical procedure, performing 3D CT reconstruction and gastric volumetry, to establish its relationship with EWL...
September 15, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28914197/characterization-of-inherent-particles-and-mechanism-of-thermal-stress-induced-particle-formation-in-hsv-2-viral-vaccine-candidate
#13
Lillian Li, Marina Kirkitadze, Kamaljit Bhandal, Cristopher Roque, Eric Yang, Bruce Carpick, Nausheen Rahman
BACKGROUND: Vaccine formulations may contain visible and/or subvisible particles, which can vary in both size and morphology. Extrinsic particles, which are particles not part of the product such as foreign contaminants, are generally considered undesirable and should be eliminated or controlled in injectable products. However, biological products, in particular vaccines, may also contain particles that are inherent to the product. Here we focus on the characterization of visible and subvisible particles in a live, replication-deficient viral vaccine candidate against HSV genital herpes in an early developmental stage...
September 14, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28904093/sodium-dynamics-in-pyramidal-neuron-dendritic-spines-synaptically-evoked-entry-predominantly-through-ampa-receptors-and-removal-by-diffusion
#14
Kenichi Miyazaki, William N Ross
Dendritic spines are key elements underlying synaptic integration and cellular plasticity, but many features of these important structures are not known or are controversial. We examined these properties with newly developed simultaneous sodium and calcium imaging that had single spine resolution in pyramidal neurons in rat hippocampal slices from either sex. Indicators for both ions were loaded through the somatic patch pipette, which also recorded electrical responses. Fluorescence changes were detected with a high speed, low noise CCD camera...
September 13, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28902989/characterizing-the-impacts-of-deposition-techniques-on-the-performance-of-mno2-cathodes-for-sodium-electrosorption-in-hybrid-capacitive-deionization
#15
Steven Hand, Roland D Cusick
Capacitive deionization (CDI) is currently limited by poor ion-selectivity and low salt adsorption capacity of porous carbon electrodes. To enhance selectivity and capacity via sodium insertion reactions, carbon aerogel electrodes were modified by depositing amorphous manganese dioxide layers via cyclic voltammetry (CV) and electroless deposition (ED). MnO2-coated electrodes were evaluated in a hybrid capacitive deionization (HCDI) system to understand the relationship between oxide coating morphology, electrode capacitance, and sodium removal efficacy...
September 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28895985/-antihistaminik-kullan%C3%A4-m%C3%A4-ile-tetiklenen-tekrarlay%C3%A4-c%C3%A4-ba%C3%A5-a%C3%A4-r%C3%A4-s%C3%A4-ve-reversible-serebral-vazokonstriksiyon-sendromu
#16
Sibel Güler, Ufuk Utku, Canan Çelebi
Reversible cerebral vasoconstriction syndrome (RCVS), also known as Call-Fleming syndrome, is one of the rare causes of thunderclap headaches, which are most often seen in females aged 20-40 years and which can cause neurological deficits. The cause of RCVS is thought to be multifocal arterial constriction and dilatation caused by transient disregulation of cerebral vascular tonus. Presently described is case of 63-year-old female patient who presented with complaint of sudden onset of recurrent headaches located on the left side...
April 2017: Aǧrı: Ağrı (Algoloji) Derneği'nin Yayın Organıdır, the Journal of the Turkish Society of Algology
https://www.readbyqxmd.com/read/28895470/generation-of-a-close-to-native-in-vitro-system-to-study-lung-cells-ecm-crosstalk
#17
Zuzana Garlikova, Ana Catarina Silva, Ana Rabata, David Potesil, Ivana Ihnatova, Jana Dumkova, Zuzana Koledova, Zbynek Zdrahal, Vladimir Vinarsky, Ales Hample, Perpetua Pinto-do-O, Diana S Nascimento
Extracellular matrix (ECM) is an essential component of the tissue microenvironment, actively shaping cellular behavior. In vitro culture systems are often poor in ECM constituents thus not allowing for naturally occurring cell-ECM interactions. This study reports on a straightforward and efficient method for generation of ECM scaffolds from lung tissue and its subsequent in vitro application using primary lung cells. Mouse lung tissue was subjected to decellularization with 0.2% sodium dodecyl sulfate, hypotonic solutions and DNase...
September 12, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28892526/rivaroxaban-vs-warfarin-sodium-in-the-ultra-early-period-after-atrial-fibrillation-related-mild-ischemic-stroke-a-randomized-clinical-trial
#18
Keun-Sik Hong, Sun U Kwon, Sang Hun Lee, Ji Sung Lee, Yong-Jae Kim, Tae-Jin Song, Young Dae Kim, Man-Seok Park, Eung-Gyu Kim, Jae-Kwan Cha, Sang Min Sung, Byung-Woo Yoon, Oh Young Bang, Woo-Keun Seo, Yang-Ha Hwang, Seong Hwan Ahn, Dong-Wha Kang, Hyun Goo Kang, Kyung-Ho Yu
Importance: In atrial fibrillation (AF)-related acute ischemic stroke, the optimal oral anticoagulation strategy remains unclear. Objective: To test whether rivaroxaban or warfarin sodium is safer and more effective for preventing early recurrent stroke in patients with AF-related acute ischemic stroke. Design, Setting, and Participants: A randomized, multicenter, open-label, blinded end point evaluation, comparative phase 2 trial was conducted from April 28, 2014, to December 7, 2015, at 14 academic medical centers in South Korea among patients with mild AF-related stroke within the previous 5 days who were deemed suitable for early anticoagulation...
September 11, 2017: JAMA Neurology
https://www.readbyqxmd.com/read/28889932/small-vessel-disease-and-dietary-salt-intake-cross-sectional-study-and-systematic-review
#19
Stephen D J Makin, Ghaida F Mubki, Fergus N Doubal, Kirsten Shuler, Julie Staals, Martin S Dennis, Joanna M Wardlaw
BACKGROUND: Higher dietary salt intake increases the risk of stroke and may increase white matter hyperintensity (WMH) volume. We hypothesized that a long-term higher salt intake may be associated with other features of small vessel disease (SVD). METHODS: We recruited consecutive patients with mild stroke presenting to the Lothian regional stroke service. We performed brain magnetic resonance imaging, obtained a basic dietary salt history, and measured the urinary sodium/creatinine ratio...
September 7, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28887954/fabrication-self-assembly-and-biomedical-applications-of-luminescent-sodium-hyaluronate-with-aggregation-induced-emission-feature
#20
Dan Wei, Yun Xue, Hongye Huang, Meiying Liu, Guangjian Zeng, Qing Wan, Liangji Liu, Jun Yu, Xiaoyong Zhang, Yen Wei
Organic dyes with aggregation-induced emission (AIE) feature have recently attracted much research attention for biomedical applications due to their inimitable optical superiority. Although many reports have demonstrated for fabricating AIE-active nanoprobes, the synthesis of AIE-active natural polymers is still very limited. In this work, fluorescent organic nanoparticles (FONs) with AIE feature have been prepared through formation of Schiff base interaction between amino-terminated AIE dye (PhE) and oxidation sodium hyaluronate (OSH)...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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