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https://www.readbyqxmd.com/read/29141760/glucose-6-phosphate-dehydrogenase-deficiency-genetic-variants-in-malaria-patients-in-southwestern-ethiopia
#1
Tamar E Carter, Seleshi Kebede Mekonnen, Karen Lopez, Victoria Bonnell, Lambodhar Damodaran, Abraham Aseffa, Daniel A Janies
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked erythrocyte enzyme disorder with relevance to malaria treatment policy. Treatment with the antimalarial primaquine can result in hemolytic anemia in G6PD-deficient patients. With increased interest in primaquine use, it is important to identify G6PD variants in Ethiopia to inform malaria treatment policy. In the present study, mutations in the G6PD gene are identified in a sample of patients with malaria in Jimma town in southwest Ethiopia. Plasmodium species of infection were confirmed using polymerase chain reaction (PCR) and gel electrophoresis...
October 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29141719/chloroquine-primaquine-versus-chloroquine-alone-to-treat-vivax-malaria-in-afghanistan-an-open-randomized-superiority-trial
#2
Ghulam Rahim Awab, Mallika Imwong, Germana Bancone, Atthanee Jeeyapant, Nicholas P J Day, Nicholas J White, Charles J Woodrow
Afghanistan's national guidelines recommend primaquine (PQ) for radical treatment of Plasmodium vivax malaria, but this is rarely implemented because of concerns over potential hemolysis in patients who have G6PD deficiency. Between August 2009 and February 2014, we conducted an open-label, randomized controlled trial of chloroquine (CQ) alone versus chloroquine plus primaquine (0.25 mg base/kg/day for 14 days) (CQ+PQ) in patients aged 6 months and older with microscopy confirmed P. vivax infection. In the CQ+PQ group, G6PD deficiency was excluded by fluorescent spot testing...
October 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29132317/development-of-amplicon-deep-sequencing-markers-and-data-analysis-pipeline-for-genotyping-multi-clonal-malaria-infections
#3
Anita Lerch, Cristian Koepfli, Natalie E Hofmann, Camilla Messerli, Stephen Wilcox, Johanna H Kattenberg, Inoni Betuela, Liam O'Connor, Ivo Mueller, Ingrid Felger
BACKGROUND: Amplicon deep sequencing permits sensitive detection of minority clones and improves discriminatory power for genotyping multi-clone Plasmodium falciparum infections. New amplicon sequencing and data analysis protocols are needed for genotyping in epidemiological studies and drug efficacy trials of P. falciparum. METHODS: Targeted sequencing of molecular marker csp and novel marker cpmp was conducted in duplicate on mixtures of parasite culture strains and 37 field samples...
November 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29131459/differential-contribution-of-interleukin-10-promoter-variants-in-malaria-and-schistosomiasis-mono-and-co-infections-among-nigerian-children
#4
Ayodele Adedoja, Nghiem Xuan Hoan, Hoang van Tong, Selorme Adukpo, Deborah B Tijani, Ajibola A Akanbi, Christian G Meyer, Olusola Ojurongbe, Thirumalaisamy P Velavan
OBJECTIVE: Interleukin-10 (IL-10) is an anti-inflammatory cytokine produced by Th1 cells and macrophages. The rationale of this study was to examine and validate possible contributions of IL-10 promoter polymorphisms in sub-Saharan Africa in children infected with either Plasmodium falciparum or Schistosoma haematobium and in children co-infected with both parasites. MATERIALS AND METHODS: 309 Nigerian children aged 4 to 15 years were recruited. The study group consisted of individuals infected either with P...
November 13, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29127650/-plasmodium-ovale-wallikeri-and-plasmodium-ovale-curtisi-malaria-in-senegal-in-2016
#5
M A Diallo, K Diongue, G Diagne, M C Seck, M Ndiaye, B Dièye, Y D Ndiaye, A S Badiane, D Ndiaye
Recently in Senegal, a case of Plasmodium ovale malaria had led to a diagnostic difficulty due to the ignorance of this parasite and the neglect of it. The objective of this study was to actively investigate cases of P. ovale malaria that would be misdiagnosed in the health centre structures of Senegal. The study was conducted in three areas that reflect different epidemiological strata of malaria. Microscopy was performed by microscopy experts on suspected malaria patients. The results were validated by Rougemont real-time PCR...
November 10, 2017: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/29115961/plasmodium-falciparum-variant-erythrocyte-surface-antigens-a-pilot-study-of-antibody-acquisition-in-recurrent-natural-infections
#6
Elise Schieck, E Jane Poole, Anja Rippert, Judy Peshu, Philip Sasi, Steffen Borrmann, Peter C Bull
BACKGROUND: During intra-erythrocytic replication Plasmodium falciparum escapes the human host immune system by switching expression of variant surface antigens (VSA). Piecemeal acquisition of variant specific antibody responses to these antigens as a result of exposure to multiple re-infections has been proposed to play a role in acquisition of naturally acquired immunity. METHODS: Immunofluorescence was used to explore the dynamics of anti-VSA IgG responses generated by children to (i) primary malaria episodes and (ii) recurrent P...
November 7, 2017: Malaria Journal
https://www.readbyqxmd.com/read/29110722/molecular-markers-for-artemisinin-and-partner-drug-resistance-in-natural-plasmodium-falciparum-populations-following-increased-insecticide-treated-net-coverage-along-the-slope-of-mount-cameroon-cross-sectional-study
#7
Tobias O Apinjoh, Regina N Mugri, Olivo Miotto, Hanesh F Chi, Rolland B Tata, Judith K Anchang-Kimbi, Eleanor M Fon, Delphine A Tangoh, Robert V Nyingchu, Christopher Jacob, Roberto Amato, Abdoulaye Djimde, Dominic Kwiatkowski, Eric A Achidi, Alfred Amambua-Ngwa
BACKGROUND: Drug resistance is one of the greatest challenges of malaria control programmes, with the monitoring of parasite resistance to artemisinins or to Artemisinin Combination Therapy (ACT) partner drugs critical to elimination efforts. Markers of resistance to a wide panel of antimalarials were assessed in natural parasite populations from southwestern Cameroon. METHODS: Individuals with asymptomatic parasitaemia or uncomplicated malaria were enrolled through cross-sectional surveys from May 2013 to March 2014 along the slope of mount Cameroon...
November 6, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29079846/hemoglobin-variants-shape-the-distribution-of-malaria-parasites-in-human-populations-and-their-transmission-potential
#8
Bronner P Gonçalves, Issaka Sagara, Mamadou Coulibaly, Yimin Wu, Mahamadoun H Assadou, Agnes Guindo, Ruth D Ellis, Mahamadou Diakite, Erin Gabriel, D Rebecca Prevots, Ogobara K Doumbo, Patrick E Duffy
Hemoglobin variants C and S protect against severe malaria but their influence on parameters not directly linked to disease severity such as gametocyte carriage and infection chronicity is less well understood. To assess whether these infection-related phenotypes depend on the host hemoglobin genotype, we followed 500 Malian individuals over 1-2 years and determined their parasitological status during monthly visits and incidental clinical episodes. While adults heterozygous for hemoglobin S mutation were less often parasitemic compared to AA adults (odds ratio [OR] 0...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29077712/quantifying-connectivity-between-local-plasmodium-falciparum-malaria-parasite-populations-using-identity-by-descent
#9
Aimee R Taylor, Stephen F Schaffner, Gustavo C Cerqueira, Standwell C Nkhoma, Timothy J C Anderson, Kanlaya Sriprawat, Aung Pyae Phyo, François Nosten, Daniel E Neafsey, Caroline O Buckee
With the rapidly increasing abundance and accessibility of genomic data, there is a growing interest in using population genetic approaches to characterize fine-scale dispersal of organisms, providing insight into biological processes across a broad range of fields including ecology, evolution and epidemiology. For sexually recombining haploid organisms such as the human malaria parasite P. falciparum, however, there have been no systematic assessments of the type of data and methods required to resolve fine scale connectivity...
October 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29063888/epidemiology-of-malaria-and-msp-2-gene-based-genetic-diversity-of-plasmodium-falciparum-from-patients-attending-community-health-centre-jiribam-manipur
#10
Indu Sharma, Appu Saikia, Paromita Chakraborty
Two millilitres of peripheral blood was collected from 323 patients of different age groups and rapid diagnosis (RDT test) was performed. Parasite genomic DNA was extracted from whole blood and MSP-2 gene-based diversity and polymorphism was determined followed by restriction fragment length polymorphism analysis using Taq 1 and Vsp 1 digestion of each polymerase chain reaction product to analyse MSP-2 genotypes. Twenty-six sequences of P. falciparum MSP-2 gene were retrieved from the current GenBank database to represent strains from various geographical locations and were analysed for the Taq 1 and Vsp 1 enzyme restriction sites using in silico restriction digestion in Sequence Manipulation Suite, Version 2...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29036178/substantial-population-structure-of-plasmodium-vivax-in-thailand-facilitates-identification-of-the-sources-of-residual-transmission
#11
Veerayuth Kittichai, Cristian Koepfli, Wang Nguitragool, Jetsumon Sattabongkot, Liwang Cui
BACKGROUND: Plasmodium vivax transmission in Thailand has been substantially reduced over the past 10 years, yet it remains highly endemic along international borders. Understanding the genetic relationship of residual parasite populations can help track the origins of the parasites that are reintroduced into malaria-free regions within the country. METHODOLOGY/RESULTS: A total of 127 P. vivax isolates were genotyped from two western provinces (Tak and Kanchanaburi) and one eastern province (Ubon Ratchathani) of Thailand using 10 microsatellite markers...
October 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29033373/evaluating-the-causal-link-between-malaria-infection-and-endemic-burkitt-lymphoma-in-northern-uganda-a-mendelian-randomization-study
#12
Ismail D Legason, Ruth M Pfeiffer, Krizia-Ivana Udquim, Andrew W Bergen, Mateus H Gouveia, Samuel Kirimunda, Isaac Otim, Eric Karlins, Patrick Kerchan, Hadijah Nabalende, Ariunaa Bayanjargal, Benjamin Emmanuel, Paul Kagwa, Ambrose O Talisuna, Kishor Bhatia, Meredith Yeager, Robert J Biggar, Leona W Ayers, Steven J Reynolds, James J Goedert, Martin D Ogwang, Joseph F Fraumeni, Ludmila Prokunina-Olsson, Sam M Mbulaiteye
BACKGROUND: Plasmodium falciparum (Pf) malaria infection is suspected to cause endemic Burkitt Lymphoma (eBL), but the evidence remains unsettled. An inverse relationship between sickle cell trait (SCT) and eBL, which supports that between malaria and eBL, has been reported before, but in small studies with low power. We investigated this hypothesis in children in a population-based study in northern Uganda using Mendelian Randomization. METHODS: Malaria-related polymorphisms (SCT, IL10, IL1A, CD36, SEMA3C, and IFNAR1) were genotyped in 202 eBL cases and 624 controls enrolled during 2010-2015...
October 3, 2017: EBioMedicine
https://www.readbyqxmd.com/read/29018842/first-detection-of-n1575y-mutation-in-pyrethroid-resistant-anopheles-gambiae-in-southern-c%C3%A3-te-d-ivoire
#13
Ako Victorien Constant Edi, Bedjou Prisca N'Dri, Mouhamadou Chouaibou, Fondjo Behi Kouadio, Patricia Pignatelli, Giovanna Raso, David Weetman, Bassirou Bonfoh
Background. The intensification of insecticide use for both public health and agriculture in Africa has contributed to growing insecticide resistance. Today, resistance to World Health Organization (WHO)-approved insecticide classes is widespread. In an agricultural area of Southern Côte d'Ivoire, the main malaria vector Anopheles coluzzii shows multiple resistance across insecticides mediated by both target site mutation and metabolic mechanisms. To plan new vector control strategies and avert future resistance liabilities caused by cross-resistance mechanisms extant within populations, it is crucial to monitor the development and spread of both resistance and mechanisms...
2017: Wellcome Open Research
https://www.readbyqxmd.com/read/29016343/passively-versus-actively-detected-malaria-similar-genetic-diversity-but-different-complexity-of-infection
#14
Zuleima Pava, Irene Handayuni, Leily Trianty, Retno A S Utami, Yusrifar K Tirta, Agatha M Puspitasari, Faustina Burdam, Enny Kenangalem, Grennady Wirjanata, Steven Kho, Hidayat Trimarsanto, Nicholas Anstey, Jeanne Rini Poespoprodjo, Rintis Noviyanti, Ric N Price, Jutta Marfurt, Sarah Auburn
The surveillance of malaria is generally undertaken on the assumption that samples passively collected at health facilities are comparable to or representative of the broader Plasmodium reservoir circulating in the community. Further characterization and comparability of the hidden asymptomatic parasite reservoir are needed to inform on the potential impact of sampling bias. This study explores the impact of sampling strategy on molecular surveillance by comparing the genetic make-up of Plasmodium falciparum and Plasmodium vivax isolates collected by passive versus active case detection...
September 5, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29016309/comparative-prevalence-of-plasmodium-falciparum-resistance-associated-genetic-polymorphisms-in-parasites-infecting-humans-and-mosquitoes-in-uganda
#15
Melissa D Conrad, Daniel Mota, Alex Musiime, Maxwell Kilama, John Rek, Moses Kamya, Grant Dorsey, Philip J Rosenthal
Controlling malaria in high transmission areas, such as much of sub-Saharan Africa, will require concerted efforts to slow the spread of drug resistance and to impede malaria transmission. Understanding the fitness costs associated with the development of drug resistance, particularly within the context of transmission, can help guide policy decisions to accomplish these goals, as fitness constraints might lead to decreased transmission of drug-resistant strains. To determine if Plasmodium falciparum resistance-mediating polymorphisms impact on development at different parasite stages, we compared the genotypes of parasites infecting humans and mosquitoes from households in Uganda...
September 25, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28993182/spatial-and-temporal-distribution-of-pfmsp1-and-pfmsp2-alleles-and-genetic-profile-change-of-plasmodium-falciparum-populations-in-gabon
#16
J M Ndong Ngomo, N P M'Bondoukwe, W Yavo, L C Bongho Mavoungou, M K Bouyou-Akotet, D P Mawili-Mboumba
Plasmodium population dynamics analysis may help to assess the impact of malaria control strategies deployment. In Gabon, new strategies have been introduced, but malaria is still a public health problem marked by a rebound of the prevalence in 2011. The aim of the study was to investigate the spatial and temporal distribution of P. falciparum strains in different areas in Gabon during a period of malaria transmission transition, between 2008 and 2011. A total of 109P. falciparum isolates were genotyped using nested-PCR of Pfmsp1 and Pfmsp2 genes...
October 7, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28992220/blood-pressure-and-arterial-stiffness-in-kenyan-adolescents-with-the-sickle-cell-trait
#17
Anthony O Etyang, Christopher K Wandabwa, Sailoki Kapesa, Esther Muthumbi, Emily Odipo, Marylene Wamukoya, Nicholas Ngomi, Tilahun Haregu, Catherine Kyobutungi, Thomas N Williams, Johnstone Makale, Alex Macharia, J Kennedy Cruickshank, Liam Smeeth, J Anthony G Scott
The potential association between sickle cell trait (SCT) and increased arterial stiffness/blood pressure (BP) has not been evaluated in detail despite its association with stroke, sudden death and renal disease. We performed 24-hour ambulatory BP monitoring and arterial stiffness measurements in adolescents raised in a malaria free environment in Kenya.Between December 2015 and June 2016, 938 randomly selected adolescents that had been continuous residents of Nairobi from birth were invited to participate in the study...
June 12, 2017: American Journal of Epidemiology
https://www.readbyqxmd.com/read/28975866/slco1a2-slco1b1-and-slco2b1-polymorphisms-influences-chloroquine-and-primaquine-treatment-in-plasmodium-vivax-malaria
#18
Vinicius A Sortica, Juliana D Lindenau, Maristela G Cunha, Maria Deise O Ohnishi, Ana Maria R Ventura, Ândrea Kc Ribeiro-Dos-Santos, Sidney Eb Santos, Luciano Sp Guimarães, Mara H Hutz
AIM: The association of transporters gene polymorphisms with chloroquine/primaquine malaria treatment response was investigated in a Brazilian population. PATIENTS & METHODS: Totally, 164 Plasmodium vivax malaria infected patients were included. Generalized estimating equations were performed to determine gene influences on parasitemia and/or gametocytemia clearance over treatment time. RESULTS: Significant interaction between SLCO2B1 genotypes and treatment over time for parasitemia clearance rate on day 2 were observed (p FDR = 0...
October 4, 2017: Pharmacogenomics
https://www.readbyqxmd.com/read/28974215/species-and-genotype-diversity-of-plasmodium-in-malaria-patients-from-gabon-analysed-by-next-generation-sequencing
#19
Albert Lalremruata, Sankarganesh Jeyaraj, Thomas Engleitner, Fanny Joanny, Annika Lang, Sabine Bélard, Ghyslain Mombo-Ngoma, Michael Ramharter, Peter G Kremsner, Benjamin Mordmüller, Jana Held
BACKGROUND: Six Plasmodium species are known to naturally infect humans. Mixed species infections occur regularly but morphological discrimination by microscopy is difficult and multiplicity of infection (MOI) can only be evaluated by molecular methods. This study investigated the complexity of Plasmodium infections in patients treated for microscopically detected non-falciparum or mixed species malaria in Gabon. METHODS: Ultra-deep sequencing of nucleus (18S rRNA), mitochondrion, and apicoplast encoded genes was used to evaluate Plasmodium species diversity and MOI in 46 symptomatic Gabonese patients with microscopically diagnosed non-falciparum or mixed species malaria...
October 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28971859/sustained-ex-vivo-susceptibility-of-plasmodium-falciparum-to-artemisinin-derivatives-but-increasing-tolerance-to-act-partner-quinolines-in-the-gambia
#20
Alfred Amambua-Ngwa, Joseph Okebe, Haddijatou Mbye, Sukai Ceesay, Fatima El-Fatouri, Fatou Joof, Nyang Haddy, Janha Ramatoulie, Muna Affara, Abdullahi Ahmad, Olimatou Kolly, Davis Nwakanma, Umberto D'Alessandro
Antimalarial interventions have yielded significant decline in malaria prevalence in The Gambia, where Artemether-Lumefantrine (AL) has been used as first line antimalarial for a decade. Clinical Plasmodium falciparum isolates collected from 2012 to 2015 were analysed ex vivo for antimalarial susceptibility and genotyped for drug resistance markers (pfcrt K76T, pfmdr1-86-184 and 1246 and pfk13) and microsatellite variation. Additionally, allele frequencies of SNPs from other drug resistance-associated genes were compared from genomic sequence datasets from 2008 (n=79) and 2014 (n=168)...
October 2, 2017: Antimicrobial Agents and Chemotherapy
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