keyword
MENU ▼
Read by QxMD icon Read
search

Malaria genotype

keyword
https://www.readbyqxmd.com/read/28214367/population-genetic-structure-and-adaptation-of-malaria-parasites-on-the-edge-of-endemic-distribution
#1
Craig W Duffy, Hampate Ba, Samuel Assefa, Ambroise D Ahouidi, Yacine B Deh, Abderahmane Tandia, Freja C M Kirsebom, Dominic P Kwiatkowski, David J Conway
To determine whether the major human malaria parasite Plasmodium falciparum exhibits fragmented population structure or local adaptation at the northern limit of its African distribution where the dry Sahel zone meets the Sahara, samples were collected from diverse locations within Mauritania over a range of ~ 1000 kilometres. Microsatellite genotypes were obtained for 203 clinical infection samples from eight locations, and Illumina paired-end sequences were obtained to yield high coverage genome-wide single nucleotide polymorphism (SNP) data for 65 clinical infection samples from four locations...
February 18, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28196496/molecular-evidence-of-plasmodium-vivax-infection-in-duffy-negative-symptomatic-individuals-from-dschang-west-cameroon
#2
Gianluca Russo, Giovanni Faggioni, Giacomo Maria Paganotti, Ghyslaine Bruna Djeunang Dongho, Alice Pomponi, Riccardo De Santis, Gianpiero Tebano, Mpoame Mbida, Martin Sanou Sobze, Vincenzo Vullo, Giovanni Rezza, Florigio Romano Lista
BACKGROUND: Plasmodium vivax infection is known to be rare in West/Central Africa, the most accepted explanation being the lack of expression of erythroid Duffy antigen in the local human populations. Duffy negativity prevents the parasite to exploit the entry mechanism on the red blood cell surface. However, there are a growing number of reported vivax infections in Duffy-negative individuals. Data on P. vivax circulation in Cameroon are limited. The aim of the study was to evaluate the P...
February 14, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28191507/mapping-the-distribution-of-anopheles-funestus-across-benin-highlights-a-sharp-contrast-of-susceptibility-to-insecticides-and-infection-rate-to-plasmodium-between-southern-and-northern-populations
#3
Rousseau Djouaka, Romaric Akoton, Genevieve M Tchigossou, Seun M Atoyebi, Helen Irving, Michael O Kusimo, Innocent Djegbe, Jacob M Riveron, Eric Tossou, Akadiri Yessoufou, Charles S Wondji
Background. Malaria remains an important public health issue in Benin, with Anopheles gambiae s.l. and Anopheles funestus s.s being the predominant vectors. This study was designed to generate information on An. funestus distribution, molecular speciation, Plasmodium infection rate and insecticide susceptibility status across Benin. Methods. Mosquito samples were collected from December 2014 to January 2016 in 46 localities in Benin. These samples were mapped and An. funestus collected were speciated to the molecular level...
December 14, 2016: Wellcome Open Res
https://www.readbyqxmd.com/read/28183301/cellulose-filtration-of-blood-from-malaria-patients-for-improving-ex-vivo-growth-of-plasmodium-falciparum-parasites
#4
Sixbert I Mkumbaye, Daniel T R Minja, Jakob S Jespersen, Michael Alifrangis, Reginald A Kavishe, Steven B Mwakalinga, John P Lusingu, Thor G Theander, Thomas Lavstsen, Christian W Wang
BACKGROUND: Establishing in vitro Plasmodium falciparum culture lines from patient parasite isolates can offer deeper understanding of geographic variations of drug sensitivity and mechanisms of malaria pathogenesis and immunity. Cellulose column filtration of blood is an inexpensive, rapid and effective method for the removal of host factors, such as leucocytes and platelets, significantly improving the purification of parasite DNA in a blood sample. METHODS: In this study, the effect of cellulose column filtration of venous blood on the initial in vitro growth of P...
February 10, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28170391/haemolysis-in-g6pd-heterozygous-females-treated-with-primaquine-for-plasmodium-vivax-malaria-a-nested-cohort-in-a-trial-of-radical-curative-regimens
#5
Cindy S Chu, Germana Bancone, Kerryn A Moore, Htun Htun Win, Niramon Thitipanawan, Christina Po, Nongnud Chowwiwat, Rattanaporn Raksapraidee, Pornpimon Wilairisak, Aung Pyae Phyo, Lily Keereecharoen, Stéphane Proux, Prakaykaew Charunwatthana, François Nosten, Nicholas J White
BACKGROUND: Radical cure of Plasmodium vivax malaria with 8-aminoquinolines (primaquine or tafenoquine) is complicated by haemolysis in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency. G6PD heterozygous females, because of individual variation in the pattern of X-chromosome inactivation (Lyonisation) in erythroid cells, may have low G6PD activity in the majority of their erythrocytes, yet are usually reported as G6PD "normal" by current phenotypic screening tests...
February 2017: PLoS Medicine
https://www.readbyqxmd.com/read/28161569/the-spread-of-artemisinin-resistant-plasmodium-falciparum-in-the-greater-mekong-subregion-a-molecular-epidemiology-observational-study
#6
Mallika Imwong, Kanokon Suwannasin, Chanon Kunasol, Kreepol Sutawong, Mayfong Mayxay, Huy Rekol, Frank M Smithuis, Tin Maung Hlaing, Kyaw M Tun, Rob W van der Pluijm, Rupam Tripura, Olivo Miotto, Didier Menard, Mehul Dhorda, Nicholas P J Day, Nicholas J White, Arjen M Dondorp
BACKGROUND: Evidence suggests that the PfKelch13 mutations that confer artemisinin resistance in falciparum malaria have multiple independent origins across the Greater Mekong subregion, which has motivated a regional malaria elimination agenda. We aimed to use molecular genotyping to assess antimalarial drug resistance selection and spread in the Greater Mekong subregion. METHODS: In this observational study, we tested Plasmodium falciparum isolates from Myanmar, northeastern Thailand, southern Laos, and western Cambodia for PfKelch13 mutations and for Pfplasmepsin2 gene amplification (indicating piperaquine resistance)...
February 1, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28143417/early-rising-asexual-parasitaemia-in-nigerian-children-following-a-first-dose-of-artemisinin-based-combination-treatments-of-falciparum-malaria
#7
Akintunde Sowunmi, Kazeem Akano, Adejumoke I Ayede, Elsie O Adewoye, Godwin Ntadom, Bayo Fatunmbi, Grace O Gbotosho, Onikepe A Folarin, Christian T Happi
BACKGROUND: Early rising asexual parasitaemia (ERAP), initially defined as 'an increase in the parasite count over the baseline pre-treatment level during the first 24 h of treatment' of falciparum malaria with artemisinin derivatives is well documented, but there is no characterization of its risk factors, kinetics, molecular features or relationship to late-appearing anaemia (LAA) in acute falciparum malaria in African children following oral artemisinin-based combination therapies (ACTs)...
January 31, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28142190/sickle-cell-trait-is-associated-with-controlled-levels-of-heme-and-mild-pro-inflammatory-response-during-acute-malaria-infection
#8
Temitope W Ademolue, Olukemi K Amodu, Gordon A Awandare
The controlled induction of hemeoxygenase-1 (HO-1), an enzyme that catabolizes heme, has been shown to reduce heme, preventing pathologies associated with heme toxicity. The hemoglobin genotype HbAS confers reduced susceptibility to severe complications of malaria by a mechanism that is not well understood. Using a longitudinal approach, we investigated the effect of baseline concentrations of HO-1 on the accumulation of heme during acute Plasmodium falciparum malaria in HbAS and HbAA genotypes. Plasma concentrations of heme, HO-1, and cytokines were quantified in venous blood obtained from children (9 months-5 years of age) during malaria infection, and at convalescence (baseline levels)...
January 31, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28137815/k13-propeller-mutations-in-plasmodium-falciparum-populations-in-malaria-endemic-regions-of-vietnam-from-2009-to-2016
#9
Nguyen Thuy-Nhien, Nguyen Kim Tuyen, Nguyen Thanh Tong, Tuong Vy, Ngo Viet Thanh, Huynh Thuy Van, Pham Huong-Thu, Huynh Hong Quang, Maciej F Boni, Christiane Dolecek, Jeremy Farrar, Guy E Thwaites, Olivo Miotto, Nicholas J White, Tran Tinh Hien
The spread of artemisinin resistant P. falciparum compromises the therapeutic efficacy of artemisinin combination therapies (ACT) and is considered the greatest threat to current global initiatives to control and eliminate malaria. This is particularly relevant for Vietnam, where dihydroartemisinin-piperaquine (DP) is the recommended ACT for P. falciparum The propeller domain gene of K13, a molecular marker of artemisinin resistance, was sequenced successfully in 1060 P. falciparum isolates collected at 3 malaria hotspots in Vietnam between 2009 and 2016...
January 30, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28115674/grammomys-surdaster-the-natural-host-for-plasmodium-berghei-parasites-as-a-model-to-study-whole-organism-vaccines-against-malaria
#10
Solomon Conteh, Charles Anderson, Lynn Lambert, Sachy Orr-Gonzalez, Jessica Herrod, Yvette L Robbins, Dariyen Carter, Stomy Bin Shamamba Karhemere, Pati Pyana, Philippe Büscher, Patrick E Duffy
Inbred mice are commonly used to test candidate malaria vaccines, but have been unreliable for predicting efficacy in humans. To establish a more rigorous animal model, we acquired African woodland thicket rats of the genus Grammomys, the natural hosts for Plasmodium berghei Thicket rats were acquired and identified as Grammomys surdaster by skull and teeth measurements and mitochondrial DNA genotyping. Herein, we demonstrate that thicket rats are highly susceptible to infection by P berghei, and moderately susceptible to Plasmodium yoelii and Plasmodium chabaudi: 1-2 infected mosquito bites or 25-100 sporozoites administered by intravenous injection consistently resulted in patent parasitemia with P...
January 23, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28114990/recent-uptake-of-intermittent-preventive-treatment-during-pregnancy-with-sulfadoxine-pyrimethamine-is-associated-with-increased-prevalence-of-pfdhfr-mutations-in-bobo-dioulasso-burkina-faso
#11
Mamoudou Cisse, Gordon A Awandare, Alamissa Soulama, Halidou Tinto, Marie-Pierre Hayette, Robert T Guiguemdé
BACKGROUND: The impact of sulfadoxine-pyrimethamine (SP) used as intermittent preventive treatment during pregnancy (IPTp-SP) on mutant parasite selection has been poorly documented in Burkina Faso. This study sought first to explore the relationship between IPTp-SP and the presence of mutant parasites. Second, to assess the relationship between the mutant parasites and adverse pregnancy outcomes. METHODS: From September to December 2010, dried blood spots (DBS) were collected during antenatal care visits and at delivery from 109 pregnant women with microscopically confirmed falciparum malaria infection...
January 23, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28103905/spatio-temporal-analysis-of-the-genetic-diversity-and-complexity-of-plasmodium-falciparum-infections-in-kedougou-southeastern-senegal
#12
Makhtar Niang, Laty G Thiam, Cheikh Loucoubar, Abdourahmane Sow, Bacary D Sadio, Mawlouth Diallo, Amadou A Sall, Aissatou Toure-Balde
BACKGROUND: Genetic analyses of the malaria parasite population and its temporal and spatial dynamics could provide an assessment of the effectiveness of disease control strategies. The genetic diversity of Plasmodium falciparum has been poorly documented in Senegal, and limited data are available from the Kedougou Region. This study examines the spatial and temporal variation of the genetic diversity and complexity of P. falciparum infections in acute febrile patients in Kedougou, southeastern Senegal...
January 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28095406/a-two-locus-model-of-the-evolution-of-insecticide-resistance-to-inform-and-optimise-public-health-insecticide-deployment-strategies
#13
Bethany Levick, Andy South, Ian M Hastings
We develop a flexible, two-locus model for the spread of insecticide resistance applicable to mosquito species that transmit human diseases such as malaria. The model allows differential exposure of males and females, allows them to encounter high or low concentrations of insecticide, and allows selection pressures and dominance values to differ depending on the concentration of insecticide encountered. We demonstrate its application by investigating the relative merits of sequential use of insecticides versus their deployment as a mixture to minimise the spread of resistance...
January 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28070005/higher-complexity-of-infection-and-genetic-diversity-of-plasmodium-vivax-than-plasmodium-falciparum-across-all-malaria-transmission-zones-of-papua-new-guinea
#14
Abebe A Fola, G L Abby Harrison, Mita Hapsari Hazairin, Céline Barnadas, Manuel W Hetzel, Jonah Iga, Peter M Siba, Ivo Mueller, Alyssa E Barry
Plasmodium falciparum and Plasmodium vivax have varying transmission dynamics that are informed by molecular epidemiology. This study aimed to determine the complexity of infection and genetic diversity of P. vivax and P. falciparum throughout Papua New Guinea (PNG) to evaluate transmission dynamics across the country. In 2008-2009, a nationwide malaria indicator survey collected 8,936 samples from all 16 endemic provinces of PNG. Of these, 892 positive P. vivax samples were genotyped at PvMS16 and PvmspF3, and 758 positive P...
January 9, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28069653/the-molecular-epidemiology-of-pf-k13-mutations-in-senegal-using-targeted-amplicon-deep-sequencing
#15
Eldin Talundzic, Yaye D Ndiaye, Awa B Deme, Christian Olsen, Dhruviben S Patel, Shweta Biliya, Rachel Daniels, Fredrik O Vannberg, Sarah K Volkman, Venkatachalam Udhayakumar, Daouda Ndiaye
The emergence of Plasmodium falciparum (Pf) resistance to artemisinin in Southeast Asia threatens malaria control and elimination activities worldwide. Multiple polymorphisms in the Pf kelch gene found in chromosome 13 (Pfk13) have been associated with artemisinin resistance. Surveillance of potential drug resistance loci within a population that may emerge under increasing drug pressure is an important public health activity. In this context, P. falciparum infections from an observational surveillance study in Senegal were genotyped using targeted amplicon deep sequencing (TADS) for Pfk13 polymorphisms...
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28067620/characterisation-of-the-opposing-effects-of-g6pd-deficiency-on-cerebral-malaria-and-severe-malarial-anaemia
#16
Geraldine M Clarke, Kirk Rockett, Katja Kivinen, Christina Hubbart, Anna E Jeffreys, Kate Rowlands, Muminatou Jallow, David J Conway, Kalifa A Bojang, Margaret Pinder, Stanley Usen, Fatoumatta Sisay-Joof, Giorgio Sirugo, Ousmane Toure, Mahamadou A Thera, Salimata Konate, Sibiry Sissoko, Amadou Niangaly, Belco Poudiougou, Valentina D Mangano, Edith C Bougouma, Sodiomon B Sirima, David Modiano, Lucas N Amenga-Etego, Anita Ghansah, Kwadwo A Koram, Michael D Wilson, Anthony Enimil, Jennifer Evans, Olukemi K Amodu, Subulade Olaniyan, Tobias Apinjoh, Regina Mugri, Andre Ndi, Carolyne M Ndila, Sophie Uyoga, Alexander Macharia, Norbert Peshu, Thomas N Williams, Alphaxard Manjurano, Nuno Sepúlveda, Taane G Clark, Eleanor Riley, Chris Drakeley, Hugh Reyburn, Vysaul Nyirongo, David Kachala, Malcolm Molyneux, Sarah J Dunstan, Nguyen Hoan Phu, Nguyen Ngoc Quyen, Cao Quang Thai, Tran Tinh Hien, Laurens Manning, Moses Laman, Peter Siba, Harin Karunajeewa, Steve Allen, Angela Allen, Timothy Me Davis, Pascal Michon, Ivo Mueller, Síle F Molloy, Susana Campino, Angeliki Kerasidou, Victoria J Cornelius, Lee Hart, Shivang S Shah, Gavin Band, Chris Ca Spencer, Tsiri Agbenyega, Eric Achidi, Ogobara K Doumbo, Jeremy Farrar, Kevin Marsh, Terrie Taylor, Dominic P Kwiatkowski
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is believed to confer protection against Plasmodium falciparum malaria, but the precise nature of the protective effecthas proved difficult to define as G6PD deficiency has multiple allelic variants with different effects in males and females, and it has heterogeneous effects on the clinical outcome of P. falciparum infection. Here we report an analysis of multiple allelic forms of G6PD deficiency in a large multi-centre case-control study of severe malaria, using the WHO classification of G6PD mutations to estimate each individual's level of enzyme activity from their genotype...
January 9, 2017: ELife
https://www.readbyqxmd.com/read/28025270/population-parameters-underlying-an-ongoing-soft-sweep-in-southeast-asian-malaria-parasites
#17
Timothy J C Anderson, Shalini Nair, Marina McDew-White, Ian H Cheeseman, Standwell Nkhoma, Fatma Bilgic, Rose McGready, Elizabeth Ashley, Aung Pyae Phyo, Nicholas J White, François Nosten
Multiple kelch13 alleles conferring artemisinin resistance (ART-R) are currently spreading through Southeast Asian malaria parasite populations, providing a unique opportunity to observe an ongoing soft selective sweep, investigate why resistance alleles have evolved multiple times and determine fundamental population genetic parameters for Plasmodium We sequenced kelch13 (n = 1,876), genotyped 75 flanking SNPs, and measured clearance rate (n = 3,552) in parasite infections from Western Thailand (2001-2014)...
January 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/27999453/frequency-of-tnfa-infg-and-il10-gene-polymorphisms-and-their-association-with-malaria-vivax-and-genomic-ancestry
#18
Adriana Antônia da Cruz Furini, Gustavo Capatti Cassiano, Marcela Petrolini Capobianco, Sidney Emanuel Batista Dos Santos, Ricardo Luiz Dantas Machado
Polymorphisms in cytokine genes can alter the production of these proteins and consequently affect the immune response. The trihybrid heterogeneity of the Brazilian population is characterized as a condition for the use of ancestry informative markers. The objective of this study was to evaluate the frequency of -1031T>C, -308G>A and -238G>A TNFA, +874 A>T IFNG and -819C>T, and -592C>A IL10 gene polymorphisms and their association with malaria vivax and genomic ancestry. Samples from 90 vivax malaria-infected individuals and 51 noninfected individuals from northern Brazil were evaluated...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27998292/genetic-evidence-that-the-makira-region-in-northeastern-madagascar-is-a-hotspot-of-malaria-transmission
#19
Benjamin L Rice, Christopher D Golden, Evelin Jean Gasta Anjaranirina, Carolina Mastella Botelho, Sarah K Volkman, Daniel L Hartl
BACKGROUND: Encouraging advances in the control of Plasmodium falciparum malaria have been observed across much of Africa in the past decade. However, regions of high relative prevalence and transmission that remain unaddressed or unrecognized provide a threat to this progress. Difficulties in identifying such localized hotspots include inadequate surveillance, especially in remote regions, and the cost and labor needed to produce direct estimates of transmission. Genetic data can provide a much-needed alternative to such empirical estimates, as the pattern of genetic variation within malaria parasite populations is indicative of the level of local transmission...
December 20, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27986683/high-rate-of-treatment-failures-in-nonimmune-travelers-treated-with-artemether-lumefantrine-for-uncomplicated-plasmodium-falciparum-malaria-in-sweden-retrospective-comparative-analysis-of-effectiveness-and-case-series
#20
Klara Sondén, Katja Wyss, Irina Jovel, Antero Vieira da Silva, Anton Pohanka, Muhammad Asghar, Manijeh Vafa Homann, Lars L Gustafsson, Urban Hellgren, Anna Färnert
BACKGROUND: Artemisinin-based combination therapy (ACT) is the first-line treatment of Plasmodium falciparum malaria. Since the introduction of artemether-lumefantrine (AL) for treatment of uncomplicated malaria in Sweden, treatment failures have been reported in adults. METHODS: A retrospective comparative analysis of treatment regimen for P. falciparum malaria in adults in Stockholm during 2000-2015 was performed to evaluate the effectiveness of AL. Parasite genotyping and drug concentrations were investigated in the AL treatment failures...
October 24, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
keyword
keyword
47930
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"