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https://www.readbyqxmd.com/read/28809142/massilia-agri-sp-nov-isolated-from-reclaimed-grassland-soil
#1
Dhiraj Kumar Chaudhary, Jaisoo Kim
A light yellow-coloured, Gram-stain-negative, motile and rod-shaped bacterium, designated strain K-3-1T, was isolated from reclaimed grassland soils of Belbari, Morang, Nepal. It was able to grow at 4-45 °C, at pH 5.0-10.0, and at 0-2 % (w/v) NaCl concentrations. This strain was taxonomically characterized by a polyphasic approach. Based on the 16S rRNA gene sequence analysis, strain K-3-1T belongs to the genus Massilia and is closely related to Massilia consociata CCUG 58010T (98.3 % sequence similarity), Massilia tieshanensis TS3T (98...
August 15, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28796903/sibling-species-of-mutualistic-symbiodinium-clade-g-from-bioeroding-sponges-in-the-western-pacific-and-western-atlantic-oceans
#2
Blake D Ramsby, Malcolm S Hill, Daniel J Thornhill, Sieuwkje F Steenhuizen, Michelle Achlatis, Allison M Lewis, Todd C Lajeunesse
Dinoflagellates in the genus Symbiodinium associate with a broad array of metazoan and protistian hosts. Symbiodinium-based symbioses involving bioeroding sponge hosts have received less attention than those involving scleractinian hosts. Certain species of common Cliona harbor high densities of an ecologically restricted group of Symbiodinium, referred to as Clade G. The relationships of these unusual Clade G Symbiodinium with Foraminifera, sponges, and black coral (Antipatharia) are rarely studied. Nonetheless, analyses of genetic evidence indicate that Clade G likely comprises several distinct species...
August 10, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28777468/technical-note-a-novel-quality-assurance-test-to-identify-gantry-angle-inaccuracies-in-respiratory-gated-vmat-treatments
#3
Emily Hubley, Michael Briscoe, Nicolas Ploquin, Greg Pierce
PURPOSE: During respiratory-gated volumetric modulated arc therapy (VMAT), the radiation beam is turned off each time the target exits the gating window. At the same time, the gantry slows, stops, and rewinds before the beam is turned back on. A quality assurance (QA) test was developed to detect inaccuracies in the gantry angle position between beam-off and beam-on events during respiratory-gated VMAT. METHODS: Strips of Gafchromic(™) EBT3 film were taped to the surface of a Capthan(®) 504 phantom mounted at isocentre...
August 4, 2017: Medical Physics
https://www.readbyqxmd.com/read/28769998/a-monte-carlo-study-of-the-photon-spectrum-due-to-the-different-materials-used-in-the-construction-of-flattening-filters-of-linac
#4
J S Estepa Jiménez, M Díaz Lagos, S A Martinez-Ovalle
Different types the spectrum of photons were studied; they were emitted from the flattening filter of a LINAC Varian 2100 C/D that operates at 15 MV. The simplified geometry of the LINAC head was calculated using the MCNPX code based on the studies of the materials of the flattening filter, namely, SST, W, Pb, Fe, Ta, Al, and Cu. These materials were replaced in the flattening filter to calculate the photon spectra at the output of this device to obtain the spectrum that makes an impact with the patient. The different spectra obtained were analyzed and compared to the emission from the original spectra configuration of the LINAC, which uses material W...
2017: Computational and Mathematical Methods in Medicine
https://www.readbyqxmd.com/read/28766904/technical-note-trog-15-01-spark-trial-multi-institutional-imaging-dose-measurement
#5
Kimberley Legge, Peter B Greer, Paul J Keall, Jeremy T Booth, Sankar Arumugam, Trevor Moodie, Doan T Nguyen, Jarad Martin, Daryl John O'Connor, Joerg Lehmann
PURPOSE: The Trans-Tasman Radiation Oncology Group (TROG) 15.01 Stereotactic Prostate Adaptive Radiotherapy utilizing Kilovoltage intrafraction monitoring (SPARK) trial is a multicenter trial using Kilovoltage Intrafraction Monitoring (KIM) to monitor prostate position during the delivery of prostate radiation therapy. KIM increases the accuracy of prostate radiation therapy treatments and allows for hypofractionation. However, an additional imaging dose is delivered to the patient. A standardized procedure to determine the imaging dose per frame delivered using KIM was developed and applied at four radiation therapy centers on three different types of linear accelerator...
August 2, 2017: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28751229/improving-efficiency-and-safety-in-external-beam-radiation-therapy-treatment-delivery-using-a-kaizen-approach
#6
Ajay Kapur, Nilda Adair, Mildred O'Brien, Nikoleta Naparstek, Thomas Cangelosi, Petrina Zuvic, Sherin Joseph, Jason Meier, Beatrice Bloom, Louis Potters
INTRODUCTION: Modern external beam radiation therapy treatment delivery processes potentially increase the number of tasks to be performed by therapists and thus opportunities for errors, yet the need to treat a large number of patients daily requires a balanced allocation of time per treatment slot. The goal of this work was to streamline the underlying workflow in such time-interval constrained processes to enhance both execution efficiency and active safety surveillance using a Kaizen approach...
June 29, 2017: Practical Radiation Oncology
https://www.readbyqxmd.com/read/28750738/dengue-virus-evades-aav-mediated-neutralizing-antibody-prophylaxis-in-rhesus-monkeys
#7
Diogo M Magnani, Michael J Ricciardi, Varian K Bailey, Martin J Gutman, Núria Pedreño-Lopez, Cassia G T Silveira, Helen S Maxwell, Aline Domingues, Lucas Gonzalez-Nieto, Qin Su, Ruchi M Newman, Melissa Pack, Mauricio A Martins, José M Martinez-Navio, Sebastian P Fuchs, Eva G Rakasz, Todd M Allen, Stephen S Whitehead, Dennis R Burton, Guangping Gao, Ronald C Desrosiers, Esper G Kallas, David I Watkins
Development of vaccines against mosquito-borne Flaviviruses is complicated by the occurrence of antibody-dependent enhancement (ADE), which can increase disease severity. Long-term delivery of neutralizing antibodies (nAbs) has the potential to effectively block infection and represents an alternative to vaccination. The risk of ADE may be avoided by using prophylactic nAbs harboring amino acid mutations L234A and L235A (LALA) in the immunoglobulin G (IgG) constant region. Here, we used recombinant adeno-associated viruses (rAAVs) to deliver the anti-dengue virus 3 (DENV3) nAb P3D05...
July 24, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28749813/preliminary-determination-of-activation-products-for-a-varian-truebeam-linear-accelerator
#8
Edward Waller, Rohan Ram, Ian Steadman
Medical linear accelerators used to treat various forms of cancers are operated at a number of different energies. A by-product of the high-energy photons produced by accelerators is activation of components within the machine itself and its surrounding bunker. The activation products pose radiological and regulatory challenges during the operation of the accelerator as well as when it is time for final decommissioning. The Varian TrueBeam is a new state-of-the-art linear accelerator now operating in the Canadian market...
September 2017: Health Physics
https://www.readbyqxmd.com/read/28732035/vaccine-induced-immune-responses-against-both-gag-and-env-improve-control-of-simian-immunodeficiency-virus-replication-in-rectally-challenged-rhesus-macaques
#9
Mauricio A Martins, Young C Shin, Lucas Gonzalez-Nieto, Aline Domingues, Martin J Gutman, Helen S Maxwell, Iris Castro, Diogo M Magnani, Michael Ricciardi, Nuria Pedreño-Lopez, Varian Bailey, Dillon Betancourt, John D Altman, Matthias Pauthner, Dennis R Burton, Benjamin von Bredow, David T Evans, Maoli Yuan, Christopher L Parks, Keisuke Ejima, David B Allison, Eva Rakasz, Glen N Barber, Saverio Capuano, Jeffrey D Lifson, Ronald C Desrosiers, David I Watkins
The ability to control lentivirus replication may be determined, in part, by the extent to which individual viral proteins are targeted by the immune system. Consequently, defining the antigens that elicit the most protective immune responses may facilitate the design of effective HIV-1 vaccines. Here we vaccinated four groups of rhesus macaques with a heterologous vector prime/boost/boost/boost (PBBB) regimen expressing the following simian immunodeficiency virus (SIV) genes: env, gag, vif, rev, tat, and nef (Group 1); env, vif, rev, tat, and nef (Group 2); gag, vif, rev, tat, and nef (Group 3); or vif, rev, tat, and nef (Group 4)...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28730652/effects-of-collimator-angle-couch-angle-and-starting-phase-on-motion-tracking-dynamic-conformal-arc-therapy-4d-dcat
#10
Zhengzheng Xu, Rutao Yao, Matthew B Podgorsak, Iris Z Wang
PURPOSE: The aim of this study was to find an optimized configuration of collimator angle, couch angle, and starting tracking phase to improve the delivery performance in terms of MLC position errors, maximal MLC leaf speed, and total beam-on time of DCAT plans with motion tracking (4D DCAT). METHOD AND MATERIALS: Nontracking conformal arc plans were first created based on a single phase (maximal exhalation phase) of a respiratory motion phantom with a spherical target...
July 21, 2017: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28721330/truebeam-radiosurgery-for-the-treatment-of-trigeminal-neuralgia-preliminary-results-at-a-single-institution
#11
Mena G Kerolus, Neilayan Sen, Sonal Mayekar, Alistar Templeton, Julius Turian, Aidnag Diaz, Lorenzo Munoz, Richard W Byrne, Sepehr Sani
INTRODUCTION: Radiosurgery is now an established method of satisfactory pain control in patients with trigeminal neuralgia (TN). The Varian Truebeam STx (Varian Medical Systems, Palo Alto, CA) linear accelerator (LINAC) system is an arc-based, frameless stereotactic radiosurgery system used for the treatment of TN. To our knowledge, there has been only one published series of patient histories that documents the use of a frameless LINAC system for the treatment of TN. We describe the treatment parameters, patient outcomes, and complications associated with the treatment of TN...
June 16, 2017: Curēus
https://www.readbyqxmd.com/read/28714067/evaluation-of-plastic-scintillator-detector-for-small-field-stereotactic-patient-specific-quality-assurance
#12
Yujiao Qin, Stephen J Gardner, Joshua Kim, Yimei Huang, Ning Wen, Anthony Doemer, Indrin J Chetty
PURPOSE: To evaluate the performance of a commercial plastic scintillator detector (PSD) for small-field stereotactic patient-specific quality assurance (QA) measurements using flattening-filter-free beam. METHODS: A total of ten spherical targets (volume range: [0.03cc-2cc]) were planned with two techniques: (1) Dynamic Conformal Arc (DCA-10 plans), and (2) Volumetric Modulated Arc Therapy (VMAT-10 plans). All plans were generated using Varian Eclipse treatment planning system, and AcurosXB v...
July 17, 2017: Medical Physics
https://www.readbyqxmd.com/read/28711479/identification-of-the-suitable-leaf-margin-for-liver-stereotactic-body-radiotherapy-with-flattening-filter-free-beams
#13
Toshiyuki Ogata, Hideki Nishimura, Hiroshi Mayahara, Kazuyuki Uehara, Takanobu Okayama
The purpose of this study is to identify the suitable leaf margin for liver stereotactic body radiotherapy (SBRT) with flattening filter-free (FFF) beams, as compared with that with flattening filter (FF) beams. SBRT treatment planning for 10 patients with liver cancer was performed using 10-MV FFF and FF beams obtained from a Varian TrueBeam (Varian Medical Systems, Palo Alto, CA) linear accelerator. Each plan was generated with the leaf margin to the planning target volume (PTV) ranging from -3 to 5 mm...
July 12, 2017: Medical Dosimetry: Official Journal of the American Association of Medical Dosimetrists
https://www.readbyqxmd.com/read/28711478/dosimetric-comparison-of-peripheral-nsclc-sbrt-using-acuros-xb-and-aaa-calculation-algorithms
#14
Chloe C H Ong, Khong Wei Ang, Roger C X Soh, Kah Ming Tin, Jerome H H Yap, James C L Lee, Christopher M Bragg
There is a concern for dose calculation in highly heterogenous environments such as the thorax region. This study compares the quality of treatment plans of peripheral non-small cell lung cancer (NSCLC) stereotactic body radiation therapy (SBRT) using 2 calculation algorithms, namely, Eclipse Anisotropic Analytical Algorithm (AAA) and Acuros External Beam (AXB), for 3-dimensional conformal radiation therapy (3DCRT) and volumetric-modulated arc therapy (VMAT). Four-dimensional computed tomography (4DCT) data from 20 anonymized patients were studied using Varian Eclipse planning system, AXB, and AAA version 10...
July 12, 2017: Medical Dosimetry: Official Journal of the American Association of Medical Dosimetrists
https://www.readbyqxmd.com/read/28708382/the-effects-of-temperature-and-hydrostatic-pressure-on-metal-toxicity-insights-into-toxicity-in-the-deep-sea
#15
Alastair Brown, Sven Thatje, Chris Hauton
Mineral prospecting in the deep sea is increasing, promoting concern regarding potential ecotoxicological impacts on deep-sea fauna. Technological difficulties in assessing toxicity in deep-sea species has promoted interest in developing shallow-water ecotoxicological proxy species. However, it is unclear how the low temperature and high hydrostatic pressure prevalent in the deep sea affect toxicity, and whether adaptation to deep-sea environmental conditions moderates any effects of these factors. To address these uncertainties we assessed the effects of temperature and hydrostatic pressure on lethal and sublethal (respiration rate, antioxidant enzyme activity) toxicity in acute (96 h) copper and cadmium exposures, using the shallow-water ecophysiological model organism Palaemon varians...
August 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28706355/the-influence-of-acquisition-mode-on-the-dosimetric-performance-of-an-amorphous-silicon-electronic-portal-imaging-device
#16
Omemh Bawazeer, Sisira Herath, Siva Sarasanandarajah, Tomas Kron, Pradip Deb
AIMS: This study investigates the impact of cine acquisition mode on the dosimetric characteristics of a Varian aS500 amorphous silicon electronic portal imaging device (a-Si EPID). MATERIALS AND METHODS: The performance of an a-Si EPID operated in cine mode was assessed and compared to its performance when operated in an integrated mode and dose measurements using an ionization chamber. This study was conducted at different photon energies and the EPID performance was assessed as function of the delivered dose, dose rate, multileaf collimator speed, field size, phantom thickness, and intensity-modulated radiation therapy fields...
April 2017: Journal of Medical Physics
https://www.readbyqxmd.com/read/28706350/investigating-the-electronic-portal-imaging-device-for-small-radiation-field-measurements
#17
Arpita Agarwal, Nikhil Rastogi, K J Maria Das, S A Yoganathan, D Udayakumar, Shaleen Kumar
PURPOSE: With the advent of state-of-the-art treatment technologies, the use of small fields has increased, and dosimetry in small fields is highly challenging. In this study, the potential use of Varian electronic portal imaging device (EPID) for small field measurements was explored for 6 and 15 MV photon beams. MATERIALS AND METHODS: The output factors and profiles were measured for a range of jaw-collimated square field sizes starting from 0.8 cm × 0.8 cm to 10 cm × 10 cm using EPID...
April 2017: Journal of Medical Physics
https://www.readbyqxmd.com/read/28693020/propiedades-biomec%C3%A3-nicas-de-la-membrana-limitante-interna-tras-recibir-tratamiento-intrav%C3%A3-treo-con-ocriplasmina
#18
Franziska Vielmuth, Ricarda G Schumann, Volker Spindler, Armin Wolf, Renate Scheler, Wolfgang J Mayer, Paul B Henrich, Christos Haritoglou
Objetivo: Evaluar la rigidez de la membrana limitante interna (MLI) humana y evaluar los posibles cambios de las propiedades mecánicas tras administrar una inyección intravítrea de ocriplasmina para tratar la tracción vitreomacular. Métodos: Este estudio se compone de una serie de casos intervencionales y comparativos de 12 muestras de MLI extraídas mediante cirugía y obtenidas de forma consecutiva de 9 ojos de 9 pacientes después de someterse sin éxito a vitreólisis farmacológica con ocriplasmina...
2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28689765/unanswered-questions-in-contemporary-heart-failure
#19
Lauren G Gilstrap, David Snipelisky, Omar AbouEzzeddine, Justin Vader, Lauren Cooper, Jacob Kelley, Antonio Perez, Kenneth Varian, Anuradha Lala, Monica Shah, Lynne W Stevenson
Background The epidemiology of heart failure (HF) is changing. This study aims to describe questions that arise during the routine care of heart failure (HF) patients that are unanswered by the current literature and describe how the type and focus of these questions has changed over time. Methods Investigators from the NHLBI-sponsored Heart Failure Apprentice Network collected and categorized questions from 5 academic hospitals over 12 months. A total of 174 unanswered questions were collected and analyzed...
July 6, 2017: Journal of Cardiac Failure
https://www.readbyqxmd.com/read/28687122/evaluation-of-ex-vivo-9-4t-mri-in-post-surgical-specimens-from-temporal-lobe-epilepsy-patients
#20
Benjamin Y M Kwan, Fateme Salehi, Ryan Kope, Donald H Lee, Manas Sharma, Robert Hammond, Jorge G Burneo, David Steven, Terry Peters, Ali R Khan
PURPOSE: This study evaluates hippocampal pathology through usage of ultra-high field 9.4T ex-vivo imaging of resected surgical specimens in patients who have undergone temporal lobe epilepsy surgery. METHOD AND MATERIALS: This is a retrospective interpretation of prospectively acquired data. MRI scanning of resected surgical specimens from patients who have undergone temporal lobe epilepsy surgery was performed on a 9.4T small bore Varian MR magnet. Structural images employed a balanced steady-state free precession sequence (TrueFISP)...
July 4, 2017: Journal of Neuroradiology. Journal de Neuroradiologie
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