Yu Fu, Xiaobai He, Quan Fang, Fei Kong, Yan Zhang, Ting Fu, Liang Chen, YanXin Liu, Zhen Wang, Jianxin Lyu, Linjie Chen
Respiratory infectious viruses, including SARS-CoV-2, undergo rapid genetic evolution, resulting in diverse subtypes with complex mutations. Detecting and differentiating these subtypes pose significant challenges in respiratory virus surveillance. To address these challenges, we integrated ARMS-PCR with molecular beacon probes, allowing selective amplification and discrimination of subtypes based on adjacent mutation sites. The method exhibited high specificity and sensitivity, detecting as low as 104 copies/mL via direct fluorescence analysis and ~106 copies/mL using real-time PCR...
March 15, 2024: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica