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Alkaline phosphatase high

S R Eman, A A Kubesy, T A Baraka, F A Torad, I S Shaymaa, Faten F Mohammed
Aim: This study was performed to evaluate the diagnostic value of hepatocyte-derived microRNA (miRNA)-122 in acute and chronic hepatitis of dogs. Materials and Methods: A total of 26 dogs presented at Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Cairo University, 16 dogs out of 26 showing clinical signs of hepatic insufficiency were subjected to clinical, ultrasonographic, hematobiochemical and ultrasound-guided fine-needle biopsy for cytological and histopathological investigations...
May 2018: Veterinary World
Rosalie M Sterner, Kimberly N Kremer, Amel Dudakovic, Jennifer J Westendorf, Andre J van Wijnen, Karen E Hedin
The bone marrow microenvironment harbors and protects leukemic cells from apoptosis-inducing agents via mechanisms that are incompletely understood. We previously showed SDF-1 (CXCL-12), a chemokine readily abundant within the bone marrow microenvironment, induces apoptosis in acute myeloid leukemia (AML) cells that express high levels of the SDF-1 receptor CXCR4. However, differentiating osteoblasts found within this niche protect cocultured AML cells from apoptosis. Additionally, this protection was abrogated upon treatment of the differentiating osteoblasts with histone deacetylase inhibitors (HDACi)...
June 18, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Guillaume Jean, Marie Hélène Lafage-Proust, Jean Claude Souberbielle, Sylvain Lechevallier, Patrik Deleaval, Christie Lorriaux, Jean Marc Hurot, Brice Mayor, Manolie Mehdi, Charles Chazot
BACKGROUND: Secondary hyperparathyroidism (SHPT) is a frequent complication of renal disease and most commonly occurs in patients on haemodialysis (HD) with metabolic, vascular, endocrine, and bone complications. The aim of this study was to analyze the evolution of mineral metabolism parameters during the first 36 months of HD treatment and identify the initial factors associated with severe SHPT. METHODS: Serum parathyroid hormone (PTH), calcium and phosphate levels were measured monthly; bone-specific alkaline phosphatase (b-ALP) and beta-CrossLaps (CTX) were measured biannually...
2018: PloS One
Patricio J Barra, Sharon Viscardi, Milko A Jorquera, Paola A Duran, Alexander J Valentine, María de la Luz Mora
Phosphobacteria, secreting organic acids and phosphatases, usually favor plant performance in acidic soils by increasing phosphorus (P) availability and aluminum (Al) complexing. However, it is not well-known how P-deficiency and Al-toxicity affect the phosphobacteria physiology. Since P and Al problems often co-occur in acidic soils, we have therefore proposed the evaluation of the single and combined effects of P-deficiency and Al-toxicity on growth, organic acids secretion, malate dehydrogenase ( mdh ) gene expression, and phosphatase activity of five Al-tolerant phosphobacteria previously isolated from ryegrass...
2018: Frontiers in Microbiology
Min Gong, Cheng Chi, Jingjing Ye, Meihong Liao, Wenqi Xie, Chengai Wu, Rui Shi, Liqun Zhang
Due to the significant role of the periosteum in bone regeneration, we hypothesised that using a specially engineered artificial periosteum could lead to an enhancement in osteogenesis in bone grafts. Herein, we describe our work aimed at fabricating an electrospun fibrous membrane as an artificial periosteum that exhibits flexibility, permeability and osteoinduction. This membrane was designed to cover the complex surface of bone grafts to facilitate and accelerate bone regeneration. The traditional Chinese medicine icariin (ICA) was subsequently introduced into poly (ε-caprolactone) (PCL) /gelatin nanofibers to fabricate an artificial periosteum for the first time...
June 14, 2018: Colloids and Surfaces. B, Biointerfaces
Samad Rahimnejad, Xiangli Yuan, Ling Wang, Kangle Lu, Kai Song, Chunxiao Zhang
This study evaluated the effects of supplementing chitooligosaccharide (COS) in low fish meal (FM) diets on growth, immune response, intestine and hepatopancrease histology, and expression of inflammatory and immune-related genes in Pacific white shrimp (Litopenaeus vannamei). A basal diet was formulated using FM and soybean meal (SM) as primary protein sources and considered as a high FM (HFM) diet, then a low FM (LFM) diet was prepared by substituting 50% of FM with SM and supplemented with 0, 3, 6, 9, 12 or 15 g COS kg-1 diet (LFM, COS3, COS6, COS9, COS12 and COS15 diets)...
June 13, 2018: Fish & Shellfish Immunology
Dani Permana, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya
The site-specific cross-linking of functional proteins creates macromolecular assemblies that exhibit unique biochemical and/or physicochemical properties. Herein, we explored the potential of laccase as a biocatalyst for the site-specific cross-linking of tyrosine-tagged proteins. Trametes sp. laccase (TL) was selected as the cross-linking catalyst, and Escherichia coli alkaline phosphatase (BAP) and antibody-binding proteins (pG2 pAs) were employed as model proteins. The protein models were genetically fused to a peptide tag containing a tyrosine residue (Y-tag) at the N- and/or C-termini...
June 12, 2018: Journal of Bioscience and Bioengineering
Maria C Spriggs, Richard W Gerhold, Rebecca P Wilkes, Paul Keenlance, Robert L Sanders, Jill Witt, Eric Clark, Debra Miller
  To better understand the clinical pathology, diseases, and causes of mortality of reintroduced American martens ( Martes americana) in Michigan, a study was conducted from 2011 to 2015 in the Upper and Lower Peninsulas of Michigan. Samples obtained from live trapping ( n = 58) or harvested carcasses ( n = 34) were serologically tested for select pathogens. Antibodies against Toxoplasma gondii and canine distemper virus were detected in 58 and 3.4% of samples, respectively. All samples were seronegative for Leptospira spp...
June 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Firuzan Firat Ozer, Selcuk Dagdelen, Tomris Erbas
The objective of this study was to investigate the effect of hyperprolactinemia and high levels of insulin-like growth factor-I (IGF-I) on bone resorption and their relation with receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) in patients with prolactinoma and acromegaly. Thirty-one patients with acromegaly, 28 patients with prolactinoma, and 33 healthy individuals were included in the study. Serum concentrations of RANKL, OPG, bone alkaline phosphatase (bone ALP), osteocalcin (OC), C-terminal telopeptide of type 1 collagen (CTX), procollagen type 1 N-terminal propeptide (P1NP) and urine deoxypyridinoline (DPD) levels were detected and bone mineral density (BMD) was measured...
June 12, 2018: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
Phyllis A Rees, R Joel Lowy
Cell lines stably transfected with genes responding to Type I interferons (IFN) are potentially a useful alternative to enzyme linked immuo-assays (ELISAs) or assays based on resistance of a test cell line to virus infection using cell death or infection endpoints. Increasingly available are a variety of commercial cell lines developed for reporter gene assays (RGAs) which are responsive to IFN exposure. These cells produce a soluble gene product which can be readily quantified using multiwell plate spectrophotometers or luminometers...
June 9, 2018: Journal of Immunological Methods
Andrew Siref, Bonnie Balzer, Andrew Hendifar
A 63-year-old white man with no significant previous medical or surgical history presented with painless jaundice after 3 weeks of dark urine, yellow stools, and a 9-pound weight loss. Bilirubin, aspartate transaminase, alanine transaminase, and alkaline phosphatase were elevated, and a computed tomography scan detected a 10-cm, ill-defined mass at the porta hepatis compressing the common bile duct. He underwent endoscopic retrograde cholangiopancreatography with stenting and ultrasound-guided biopsies. Histologic sections showed a neoplastic population of small ovoid cells with a high N:C ratio, nuclear hyperchromasia, "smoky" chromatin and abundant mitotic figures, and characteristic of high-grade neuroendocrine carcinoma (HGNECA)...
June 7, 2018: American Journal of Dermatopathology
JingJing Zhang, Yi Lu
While biocomputing is recognized to provide intelligent solution to complex biosensing projects, it remains challenging to transform biomolecular logic gates into a convenient, portable, resettable and quantitative sensing system for point-of-care (POC) diagnostics in a low-resource setting. To overcome these limitations, we herein report the first design and demonstration of biocomputing on personal glucose meters (PGM) by utilizing glucose and nicotinamide adenine dinucleotide as signal outputs, DNAzyme and protein enzymes as building blocks and demonstrating a general platform for installing logic-gate responses (YES, NOT, INHIBIT and OR) to a variety of biological species, such as cations (sodium), anions (citrate), organic metabolites (ADP, ATP) and enzymes (pyruvate kinase, alkaline phosphatase, alcohol dehydrogenases)...
June 12, 2018: Angewandte Chemie
Monika Lopuszanska-Dawid
Background: Mental factors may influence physical functioning and psychological well-being working positively on physical health. The aim of this study is to investigate the relationship between personal life satisfaction (LS) and biological condition in healthy males and females in Poland. Material and methods: The participants were 2102 men and 1397 women, 25-65 years of age, inhabitants of Wroclaw, Poland. LS was used as a measure of subjective well-being. Biological condition was evaluated by 13 objective medical measures characterizing a condition of the cardiovascular system, biochemistry, fitness and a level and distribution of general body adiposity...
June 11, 2018: Anthropologischer Anzeiger; Bericht über die Biologisch-anthropologische Literatur
Jun-Qiang Yin, Yi-Wei Fu, Xian-Biao Xie, Xiao-Yu Cheng, Xiao-Yu Yang, Wei-Hai Liu, Jian Tu, Zhen-Hua Gao, Jing-Nan Shen
Background and purpose: Telangiectatic osteosarcoma (TOS), a rare variant of osteosarcoma, may be easily misdiagnosed as aneurysmal bone cyst (ABC). The aims of this study were to investigate the diagnostic and prognostic factors of TOS by reviewing our experience with TOS and to develop a diagnostic model that may distinguish TOS from ABC. Materials and methods: We identified 51 cases of TOS treated at the First Affiliated Hospital of Sun Yat-Sen University from March 2001 to January 2016 and reviewed their records, imaging information and pathological studies...
June 2018: Journal of Bone Oncology
Reinhold G Erben
Fibroblast growth factor-23 (FGF23) is a bone-derived hormone suppressing phosphate reabsorption and vitamin D hormone synthesis in the kidney. At physiological concentrations of the hormone, the endocrine actions of FGF23 in the kidney are αKlotho-dependent, because high-affinity binding of FGF23 to FGF receptors requires the presence of the co-receptor αKlotho on target cells. It is well established that excessive concentrations of intact FGF23 in the blood lead to phosphate wasting in patients with normal kidney function...
2018: Frontiers in Endocrinology
Yahu Hu, Yu Huang, Jieqiong Su, Zhuo Gao, Shuqi Li, Zhongren Nan
Metal bioavailability and extracellular enzyme activity are two important indicators of soil quality in metal-contaminated soil. However, it is unclear how the chronosequence effect modifies these two factors in highly contaminated calcareous soils undergoing afforestation. We used Populus simonii Carr. and the calciphilous Ulmus macrocarpa Hance as contrasting tree species to study the chronosequence effect. We found that afforestation significantly increased soil total nitrogen (N) content as well as soil carbon (C)/phosphorus (P) and N/P ratios, but decreased soil total P content and soil C/N ratio, regardless of the tree species and stand age, suggesting strong P limitation...
May 1, 2018: Science of the Total Environment
Xia Zhang, Jingping Zhang, Yuan Shen, Changhao Zhou, Xiaoping Huang
Previous studies conducted on Daya Bay implied that the bay had been undergoing potential phosphorus limitation. In this context, alkaline phosphatase activity (APA) and the associated microbes were investigated in three different seasons in Daya Bay, South China Sea. Both bulk-community (fractioned into dissolved and particulate) and single-cell assays of APA were used to estimate the P status of phytoplankton at the community and species level. Unexpected high potential APA (Vmax ) was observed in Daya Bay...
June 2018: Marine Pollution Bulletin
Junfa Yin, Shaokun Chen, Ning Zhang, Hailin Wang
Identification and quantification of chemical DNA modifications provides essential information on genomic DNA changes, e.g., epigenetic modifications and abnormal DNA lesions. In this vein, it requires to digest genomic DNA strands into single nucleosides, facilitating the mass spectrometry analysis. However, rapid digestion of such supramacromolecules DNA of several millions Daltons (M.W.) into single nucleosides remains very challenging. Here we constructed an immobilized ben-zonase capillary bioreactor, and further tandemly coupled with immobilized snake venom phosphodiesterase (SVP) and alkaline phosphatase (ALPase) capillary bioreactor to form a novel three-enzyme cascade bioreactor (BenzoSAC bioreac-tor)...
June 8, 2018: ACS Applied Materials & Interfaces
Mariusz Cycoń, Kamila Orlewska, Anna Markowicz, Agnieszka Żmijowska, Joanna Smoleń-Dzirba, Jolanta Bratosiewicz-Wąsik, Tomasz J Wąsik, Zofia Piotrowska-Seget
Despite many studies, our knowledge on the impact of antibiotics and antibiotic-resistant bacteria on the metabolic activity of soil microbial communities is still limited. To ascertain this impact, the community level physiological profiles (CLPPs) and the activity of selected enzymes (dehydrogenase, urease, and phosphatases) in soils treated with vancomycin (VA) and/or multidrug resistant Citrobacter freundii were determined during a 90-day experiment. A multivariate analysis and the resistance (RS)/resilience (RL) concept were used to assess the potential of native microorganisms to maintain their catabolic activity under exposure of VA and/or a high level of C...
2018: Frontiers in Microbiology
Christophe Corpechot, Olivier Chazouillères, Alexandra Rousseau, Antonia Le Gruyer, François Habersetzer, Philippe Mathurin, Odile Goria, Pascal Potier, Anne Minello, Christine Silvain, Armand Abergel, Maryline Debette-Gratien, Dominique Larrey, Olivier Roux, Jean-Pierre Bronowicki, Jérôme Boursier, Victor de Ledinghen, Alexandra Heurgue-Berlot, Eric Nguyen-Khac, Fabien Zoulim, Isabelle Ollivier-Hourmand, Jean-Pierre Zarski, Gisèle Nkontchou, Sara Lemoinne, Lydie Humbert, Dominique Rainteau, Guillaume Lefèvre, Luc de Chaisemartin, Sylvie Chollet-Martin, Farid Gaouar, Farid-Hakeem Admane, Tabassome Simon, Raoul Poupon
BACKGROUND: Patients with primary biliary cholangitis who have an inadequate response to therapy with ursodeoxycholic acid are at high risk for disease progression. Fibrates, which are agonists of peroxisome proliferator-activated receptors, in combination with ursodeoxycholic acid, have shown potential benefit in patients with this condition. METHODS: In this 24-month, double-blind, placebo-controlled, phase 3 trial, we randomly assigned 100 patients who had had an inadequate response to ursodeoxycholic acid according to the Paris 2 criteria to receive bezafibrate at a daily dose of 400 mg (50 patients), or placebo (50 patients), in addition to continued treatment with ursodeoxycholic acid...
June 7, 2018: New England Journal of Medicine
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