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https://www.readbyqxmd.com/read/27909781/the-ability-of-low-magnitude-mechanical-signals-to-normalize-bone-turnover-in-adolescents-hospitalized-for-anorexia-nervosa
#1
A D DiVasta, H A Feldman, C T Rubin, J S Gallagher, N Stokes, D P Kiel, B D Snyder, C M Gordon
: We sought to determine whether low-magnitude mechanical stimulation (LMMS) normalizes bone turnover among adolescents hospitalized for anorexia nervosa (AN). Brief, daily LMMS prevents the decline in bone turnover typically seen during bed rest in AN. LMMS may have application for patients with AN in the inpatient setting to protect bone health. INTRODUCTION: Malnourished adolescents with AN requiring medical hospitalization are at high risk for rapid reduction in skeletal quality...
December 1, 2016: Osteoporosis International
https://www.readbyqxmd.com/read/27905856/1-25-dihydroxyvitamin-d3-stimulates-odontoblastic-differentiation-of-human-dental-pulp-stem-cells-in-vitro
#2
Goksen Mucuk, Elif Sepet, Mine Erguven, Ozlem Ekmekci, Ayhan Bilir
BACKGROUND: 1,25-dihydroxyvitamin D3 (1,25-OH D3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). AIM: This study aimed to understand the influence of various concentrations of 1,25-OH D3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. MATERIALS AND METHODS: hDPSCs were obtained from the impacted third molar teeth...
December 1, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27897998/a-new-two-photon-ratiometric-fluorescent-probe-for-detecting-alkaline-phosphatase-in-living-cells
#3
Xiaohong Zhou, Yuren Jiang, Xiongjie Zhao, Yao Zhu
Alkaline phosphatase (ALP) is an important diagnostic indicator of many human diseases. To quantitatively track ALP in biosystems, herein, for the first time, we report an efficient two-photon ratiometric fluorescent probe, termed probe 1 and based on classic naphthalene derivatives with a donor-π-acceptor (D-π-A) structure and deprotection of the phosphoric acid moiety by ALP. The presence of ALP causes the cleave of the phosphate group from naphthalene derivatives and the phosphate group changes the ability of the intramolecular charge transfer (ICT) and remarkably alters the probe's photophysical properties, thus an obvious ratiometric signal with an isoemissive point is observed...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27896593/biochemical-and-oxidative-stress-markers-in-the-liver-and-kidneys-of-rats-submitted-to-different-protocols-of-anabolic-steroids
#4
Guilherme Lopes Dornelles, Andressa Bueno, Juliana Sorraila de Oliveira, Aleksandro Schafer da Silva, Raqueli Teresinha França, Cássia Bagolin da Silva, Márcia Silveira Netto Machado, Letícia do Santos Petry, Fátima Husein Abdalla, Cibele Lima Lhamas, Cinthia Melazzo de Andrade
The objective of this study was to evaluate the effects of different protocols (P1, P2, and P3) of boldenone undecylenate (BU) and stanozolol (ST) on markers of liver and kidney function and variables of oxidative stress in these organs. For this, 54 male Wistar rats were divided into nine groups of six animals each. Each animal received intramuscularly 5.0 mg kg(-1) of BU or ST once a week for 4 weeks (P1); 2.5 mg kg(-1) of BU or ST once a week for 8 weeks (P2); and 1.25 mg kg(-1) of BU or ST once a week for 12 weeks (P3)...
November 28, 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27893751/25-hydroxy-and-1%C3%AE-25-dihydroxycholecalciferol-have-greater-potencies-than-25-hydroxy-and-1%C3%AE-25-dihydroxyergocalciferol-in-modulating-cultured-human-and-mouse-osteoblast-activities
#5
Allahdad Zarei, Philippa A Hulley, Afsie Sabokbar, M Kassim Javaid, Alireza Morovat
Despite differences in the phamacokinetics of 25-hydroxycholecalciferol (25(OH)D3) and 25-hydroxyergocalciferol (25(OH)D2) in man, the effects of these and their 1α-hydroxylated forms (1,25(OH)2D3 and 1,25(OH)2D2) on cellular activity of vitamin D-responsive cells have hardly been compared. We studied differences in the effects of these metabolites on cell number, gene transcription, protein expression and mineralisation of cultured human bone marrow-derived stromal cells (hBMSC) and rapidly mineralising mouse 2T3 osteoblasts...
2016: PloS One
https://www.readbyqxmd.com/read/27887608/role-of-ncan-rs2228603-polymorphism-in-the-incidence-of-nonalcoholic-fatty-liver-disease-a-case-control-study
#6
Meng-Juan Wu, Chen Yuan, Lin-Lin Lu, Bai-Quan An, Shi-Ying Xuan, Yong-Ning Xin
BACKGROUND: Recently genome-wide association studies identified that NCAN rs2228603 polymorphism was associated with non-alcoholic fatty liver disease (NAFLD) mainly in subjects of European ancestry. While no research have been conducted to demonstrate the relationship between NCAN rs2228603 and NAFLD in Chinese Han adults. The aim of this study was to investigate whether NCAN rs2228603 is associated with NAFLD in Chinese population. METHODS: Gene NCAN rs2228603 was genotyped in 182 patients with NAFLD and 195 healthy controls...
November 26, 2016: Lipids in Health and Disease
https://www.readbyqxmd.com/read/27886764/sensitive-and-selective-colorimetric-assay-of-alkaline-phosphatase-activity-with-cu-ii-phenanthroline-complex
#7
Qiong Hu, Minhui He, Yaqi Mei, Wenjie Feng, Su Jing, Jinming Kong, Xueji Zhang
Alkaline phosphatase (ALP) plays a vital role in dephosphorylation- and phosphorylation-related cellular regulation and signaling processes. Accordingly, the development of efficient methods for ALP activity assay is of significant importance in clinical diagnosis. In this work, a simple and practical method is reported for the first time for the sensitive and selective colorimetric assay of ALP activity by exploiting a water-soluble Cu(II)-phenanthroline complex as the probe, on the basis of the distinctive metal-to-ligand charge-transfer (MLCT) properties...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27885785/osteogenic-activity-and-antibacterial-effect-of-porous-titanium-modified-with-metal-organic-framework-films
#8
Junyu Chen, Xin Zhang, Chao Huang, He Cai, Shanshan Hu, Qianbing Wan, Xibo Pei, Jian Wang
As a new class of crystalline nanoporous materials, metal-organic frameworks (MOFs) have recently been used for biomedical applications due to their large surface area, high porosity, and theoretically infinite structures. To improve the biological performance of titanium, MOF films were applied to surface modification of titanium. Zn-based MOF films composed of zeolitic imidazolate framework-8 (ZIF-8) crystals with nanoscale and microscale sizes (nanoZIF-8 and microZIF-8) were prepared on porous titanium surfaces by hydrothermal and solvothermal methods, respectively...
November 5, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27885436/effects-of-aluminum-trichloride-on-the-cartilage-stimulatory-growth-factors-in-rats
#9
Fan Zhang, Xudong Sun, Hongyan Yu, Xu Yang, Miao Song, Yanfei Han, Yanfei Li, Yanzhu Zhu
Aluminum (Al) is considered to be a potentially toxic metal and inhibits cartilage formation. Transforming growth factor β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2) are cartilage stimulatory growth factors, which play important roles in regulating the cartilage formation. To investigate the effects of aluminum trichloride (AlCl3) on the regulation of cartilage formation. Eighty Wistar rats were orally exposed to 0 (control group), 0.4 g/L (low-dose group), 0.8 g/L (mid-dose group) and 1.6 g/L (high-dose group) AlCl3 for 120 days, respectively...
November 24, 2016: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/27884524/increasing-phytoplankton-available-phosphorus-and-inhibition-of-macrophyte-on-phytoplankton-bloom
#10
Yanran Dai, Juan Wu, Xiaohang Ma, Fei Zhong, Naxin Cui, Shuiping Cheng
We assembled mesocosms to address the coherent mechanisms that an increasing phosphorus (P) concentration in water columns coupled with the phytoplankton bloom and identify the performance gap of regulating phytoplankton growth between two macrophyte species, Ceratophyllum demersum L. and Vallisneria spiralis L. Intense alkaline phosphatase activities (APA) were observed in the unplanted control, with their predominant part, phytoplankton APA (accounting for up to 44.7% of the total APA), and another large share, bacterial APA...
November 22, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27883132/microfluidic-pdms-on-paper-pop-devices
#11
Jin-Wen Shangguan, Yu Liu, Jian-Bin Pan, Bi-Yi Xu, Jing-Juan Xu, Hong-Yuan Chen
In this paper, we propose a generalized concept of microfluidic polydimethylsiloxane (PDMS) on paper (POP) devices, which combines well the merits of paper chips and PDMS chips. First, we optimized the conditions for accurate PDMS spatial patterning on paper, based on screen printing and a high temperature enabled superfast curing technique, which enables PDMS patterning to an accuracy of tens of microns in less than ten seconds. This, in turn, makes it available for seamless, reversible and reliable integration of the resulting paper layer with other PDMS channel structures...
November 24, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27878265/synergistic-effects-of-overexpression-of-bmp%C3%A2-2-and-tgf%C3%A2-%C3%AE-3-on-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells
#12
Yilin Wang, Tian He, Jie Liu, Hongzhi Liu, Lugang Zhou, Wei Hao, Yujie Sun, Xin Wang
Bone morphogenetic protein 2 (BMP-2) and transforming growth factor β (TGF-β) isoforms are important in advancing bone regeneration. The aim of the present study was to investigate the positive and reciprocal effect of TGF‑β3, one of the three TGF‑β isoforms, on BMP‑2 in promoting osteogenic differentiation. Exogenous BMP‑2 and TGF‑β3 genes were separately, and in combination, overexpressed in rabbit bone marrow‑derived mesenchymal stem cells (rBMSCs). Expression levels of BMP‑2 and TGF‑β3 were evaluated using reverse‑transcription‑polymerase chain reaction (RT‑PCR) and Western blotting assays...
November 22, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27873074/a-cross-sectional-study-of-the-association-between-adipokine-levels-and-bone-mineral-density-according-to-obesity-and-menopausal-status-in-korean-women
#13
Ji-Hee Haam, Young-Sang Kim, Moon Jong Kim, Hyung Suk Koo, Hyung Yuk Kim, Hye-Jung Kim, Ki Hyun Park, Nam-Seok Joo, Kyung-Chae Park
Results regarding the association between adipokine levels and bone mineral density (BMD) have been inconsistent; the effects of sex, menopause, and central obesity remain unknown. We evaluated the association between serum leptin, adiponectin, and high-molecular-weight (HMW) adiponectin levels and BMD according to menopause and central obesity status in Korean women. This cross-sectional study comprised 255 women undergoing examinations at the CHA Bundang Medical Center. Participants were divided according to menopause, and central obesity status...
November 21, 2016: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/27872971/the-influence-of-nitrogen-on-the-biological-properties-of-soil-contaminated-with-zinc
#14
Rafał Strachel, Jadwiga Wyszkowska, Małgorzata Baćmaga
This study analyzed the relationship between nitrogen fertilization and the biological properties of soil contaminated with zinc. The influence of various concentrations of zinc and nitrogen on the microbiological and biochemical activity of soil was investigated. In a laboratory experiment, loamy sand with pHKCl 5.6 was contaminated with zinc (ZnCl2) and fertilized with urea as a source of nitrogen. The activity of acid phosphatase, alkaline phosphatase, urease and β-glucosidase, and microbial counts were determined in soil samples after 2 and 20 weeks of incubation...
November 21, 2016: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/27871616/a-sensitive-fluorescence-biosensor-for-alkaline-phosphatase-activity-based-on-the-cu-ii-dependent-dnazyme
#15
Mengmeng Zhao, Yajuan Guo, Lixu Wang, Fang Luo, Cuiying Lin, Zhenyu Lin, Guonan Chen
Alkaline phosphatase (ALP) plays an important role in phosphate metabolism processes; deviation from its normal level may indicate different kinds of diseases, so it is highly necessary to develop some simple and sensitive methods to monitor the ALP level. In this study, a simple, high selective, and sensitive fluorescent biosensor has been proposed for ALP activity determination. The Cu(II)-dependent DNAzyme (Cu-Enzyme) are divided into two parts: Cu-Enzyme 1 and Cu-Enzyme 2, and labelled with alkyne and azido groups, respectively...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27871004/bde-47-exposure-changed-the-immune-function-of-haemocytes-in-mytilus-edulis-an-explanation-based-on-ros-mediated-pathway
#16
Yongshun Jiang, Xuexi Tang, Tianli Sun, You Wang
Brominated Tetra-BDE (BDE-47), is suggested to be widely distributed in marine environments and highly accumulated in marine organisms. Blue mussel Mytilus edulis is a sentinel organism that is commonly used for monitoring chemical contaminants in coastal ecosystems, and its haemocytes play an essential role in immune function. Therefore, we estimated the effects of BDE-47 exposure on the M. edulis haemocytes' immune function under controlled laboratory conditions. The study found the following results: (1) BDE-47 exposure increased the mortality of the haemocytes and decreased the total haemocyte counts...
November 11, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27866237/long-term-effects-of-intravenous-ibandronate-in-paget-s-disease-of-bone
#17
Ian R Reid, Diana Wattie, Gregory D Gamble, Ramanamma Kalluru, Tim Cundy
We have previously demonstrated that intravenous ibandronate produces high initial response rates in Paget's disease, but the durability of this effect is unknown. It might be expected to be short lived because ibandronate has a low affinity for bone. Here we report long-term follow-up (up to 14 years) of patients from that trial. Twenty-five patients with active Paget's disease [baseline serum total alkaline phosphatase (ALP) ~3 times the upper limit of normal] received either 6 or 12 mg intravenous ibandronate at baseline...
November 19, 2016: Calcified Tissue International
https://www.readbyqxmd.com/read/27865936/constitutive-%C3%AE-catenin-activation-in-osteoblasts-impairs-terminal-osteoblast-differentiation-and-bone-quality
#18
Quanwei Bao, Sixu Chen, Hao Qin, Jianquan Feng, Huayu Liu, Daocheng Liu, Ang Li, Yue Shen, Xiaozheng Zhong, Junfeng Li, Zhaowen Zong
Accumulating evidence suggests that Wnt/β-catenin signaling plays a central role in controlling bone mass. We previously reported that constitutive activation of β-catenin (CA-β-catenin) in osteoblasts potentially has side effects on the bone growth and bone remodeling process, although it could increase bone mass. The present study aimed to observe the effects of osteoblastic CA-β-catenin on bone quality and to investigate possible mechanisms of these effects. It was found that CA-β-catenin mice exhibited lower mineralization levels and disorganized collagen in long bones as confirmed by von Kossa staining and sirius red staining, respectively...
November 17, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27865621/menaquinone-4-vitamin-k2-up-regulates-expression-of-human-intestinal-alkaline-phosphatase-in-caco-2-cells
#19
Seiko Noda, Asako Yamada, Rieko Tanabe, Kanae Nakaoka, Takayuki Hosoi, Masae Goseki-Sone
Alkaline phosphatase (ALP) hydrolyzes several monophosphate esters into inorganic acid and alcohol. In humans, 4 kinds of ALP isozymes have been identified: tissue-nonspecific ALP, intestinal ALP, placental ALP, and germ cell ALP. Intestinal ALP is expressed at a high concentration in the brush border membrane of intestinal epithelial cells and is known to be affected by several kinds of nutrients, such as lipids, but the physiological function of intestinal ALP has remained elusive. Vitamin K is an essential cofactor for the posttranslational carboxylation of glutamate residues into γ-carboxy glutamate...
October 14, 2016: Nutrition Research
https://www.readbyqxmd.com/read/27861869/s100b%C3%A2-%C3%A2-a1-celisa-a-novel-potency-assay-and-screening-tool-for-redifferentiation-stimuli-of-human-articular-chondrocytes
#20
Jose Diaz-Romero, Sibylle Kürsener, Sandro Kohl, Dobrila Nesic
During monolayer expansion, a necessary step in autologous chondrocyte implantation, human articular chondrocytes (HAC) dedifferentiate and lose their capacity to produce stable hyaline cartilage. Determining HAC potency and learning how to trigger their redifferentiation would improve cell-based cartilage regeneration therapies. We previously identified S100B and S100A1 proteins as markers of HAC redifferentiation potential. Here, we aimed to: (i) demonstrate a correlation between S100B + A1-positive HAC in monolayer culture and their neochondrogenesis capacity in pellet culture; (ii) develop an S100B + A1 cell-based ELISA, and (iii) prove that S100B + A1 induction in HAC increases their chondrogenic capacity...
November 11, 2016: Journal of Cellular Physiology
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