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https://www.readbyqxmd.com/read/28076853/pyruvate-dehydrogenase-expression-is-negatively-associated-with-cell-stemness-and-worse-clinical-outcome-in-prostate-cancers
#1
Yali Zhong, Xiaoli Li, Yasai Ji, Xiaoran Li, Yaqing Li, Dandan Yu, Yuan Yuan, Jian Liu, Huixiang Li, Mingzhi Zhang, Zhenyu Ji, Dandan Fan, Jianguo Wen, Mariusz Adam Goscinski, Long Yuan, Bin Hao, Jahn M Nesland, Zhenhe Suo
Cells generate adenosine-5'-triphosphate (ATP), the major currency for energy-consuming reactions, through mitochondrial oxidative phosphorylation (OXPHOS) and glycolysis. One of the remarkable features of cancer cells is aerobic glycolysis, also known as the "Warburg Effect", in which cancer cells rely preferentially on glycolysis instead of mitochondrial OXPHOS as the main energy source even in the presence of high oxygen tension. One of the main players in controlling OXPHOS is the mitochondrial gatekeeperpyruvate dehydrogenase complex (PDHc) and its major subunit is E1α (PDHA1)...
January 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/28068639/effects-of-argan-oil-on-the-mitochondrial-function-antioxidant-system-and-the-activity-of-nadph-generating-enzymes-in-acrylamide-treated-rat-brain
#2
Birsen Aydın
Argan oil (AO) is rich in minor compounds such as polyphenols and tocopherols which are powerful antioxidants. Acrylamide (ACR) has been classified as a neurotoxic agent in animals and humans. Mitochondrial oxidative stress and dysfunction is one of the most probable molecular mechanisms of neurodegenerative diseases. Female Sprague Dawley rats were exposed to ACR (50mg/kg i.p. three times a week), AO (6ml/kg,o.p, per day) or together for 30days. The activities of cytosolic enzymes such as xanthine oxidase (XO), glucose 6-phosphate dehydrogenase (G6PDH), glutathione-S-transferase (GST), mitochondrial oxidative stress, oxidative phosphorylation (OXPHOS) and tricarboxylic acid cycle (TCA) enzymes, mitochondrial metabolic function, adenosine triphosphate (ATP) level and acetylcholinesterase (AChE) activity were assessed in rat brain...
January 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28068289/cancer-metabolism-the-volatile-signature-of-glycolysis-in-vitro-model-in-lung-cancer-cells
#3
Tali Feinberg, Jens Herbig, Ingrid Kohl, Guy Las, John C Cancilla, Jose S Torrecilla, Maya Ilouze, Hossam Haick, Nir Peled
Discovering the volatile signature of cancer cells is an emerging approach in cancer research, as it may contribute to a fast and simple diagnosis of tumors in vivo and in vitro. One of the main contributors to such a volatile signature is hyperglycolysis, which characterizes the cancerous cell. The metabolic perturbation in cancer cells is known as the Warburg effect; glycolysis is preferred over oxidative phosphorylation (OXPHOS), even in the presence of oxygen. The precise mitochondrial alterations that underlie the increased dependence of cancer cells on aerobic glycolysis for energy generation have remained a mystery...
January 9, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28065746/linking-metabolic-reprogramming-to-therapy-resistance-in-cancer
#4
REVIEW
Andrea Morandi, Stefano Indraccolo
Metabolic rearrangements are essential to satisfy the different requirements of cancer cells during tumorigenesis and recent studies have highlighted a role for such metabolic reprogramming in response and adaptation to therapies. However, therapy-resistant experimental models have been described to be either glycolysis-dependent or OXPHOS-addicted. Here we discuss the recent literature on metabolic reprogramming of cancer in therapy resistance with a plausible explanation of the observed differences which collectively indicate that dis-regulated metabolic pathways could be considered a potential therapeutic target in tumors resistant to conventional therapy...
January 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28064117/differential-proteomic-and-oxidative-profiles-unveil-dysfunctional-protein-import-to-adipocyte-mitochondria-in-obesity-associated-aging-and-diabetes
#5
María Gómez-Serrano, Emilio Camafeita, Juan A López, Miguel A Rubio, Irene Bretón, Inés García-Consuegra, Eva García-Santos, Jesús Lago, Andrés Sánchez-Pernaute, Antonio Torres, Jesús Vázquez, Belén Peral
Human age-related diseases, including obesity and type 2 diabetes (T2DM), have long been associated to mitochondrial dysfunction; however, the role for adipose tissue mitochondria in these conditions remains unknown. We have tackled the impact of aging and T2DM on adipocyte mitochondria from obese patients by quantitating not only the corresponding abundance changes of proteins, but also the redox alterations undergone by Cys residues thereof. For that, we have resorted to a high-throughput proteomic approach based on isobaric labeling, liquid chromatography and mass spectrometry...
December 18, 2016: Redox Biology
https://www.readbyqxmd.com/read/28049038/trimethylamine-n-oxide-impairs-pyruvate-and-fatty-acid-oxidation-in-cardiac-mitochondria
#6
Marina Makrecka-Kuka, Kristine Volska, Unigunde Antone, Reinis Vilskersts, Solveiga Grinberga, Dace Bandere, Edgars Liepinsh, Maija Dambrova
Increased plasma concentration of trimethylamine N-oxide (TMAO), a proatherogenic metabolite, has been linked to adverse cardiovascular outcomes; however, it remains unclear whether TMAO is a biomarker or whether it induces direct detrimental cardiovascular effects. Because altered cardiac energy metabolism and mitochondrial dysfunction play crucial roles in the development of cardiovascular diseases, we hypothesized that increased TMAO concentration may alter mitochondrial energy metabolism. The aim of the present study was to determine the effects of TMAO on cardiac mitochondrial energy metabolism...
December 31, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/28034293/low-concentrations-of-cationic-pamam-dendrimers-affect-lymphocyte-respiration-in-in-vitro-studies
#7
Magdalena Labieniec-Watala, Marzena Szwed, Joanna Hertel, Ewelina Wisnik
BACKGROUND: The effect of low concentrations of poly(amido)amine dendrimers (G2-G4) on human lymphocytes was studied. Some works revealed that PAMAMs can adversely affect the morphology of blood components and mitochondria functions. In this context, the present report aimed to investigate the in vitro cationic dendrimers' effect on mitochondrial respiration and cell morphology in lymphocytes isolated from human blood. METHODS: To monitor the mitochondria changes, the high-resolution respirometer was used...
December 29, 2016: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28028849/butyrate-stimulates-adipose-lipolysis-and-mitochondrial-oxphos-through-histone-hyperacetylation-associated-ar3%C3%AE-activation-in-high-fat-diet-induced-obese-mice
#8
Yimin Jia, Jian Hong, Huifang Li, Yun Hu, Longfei Jia, Demin Cai, Ruqian Zhao
Butyrate can prevent diet-induced obesity through increasing energy expenditure. However, it is unclear whether AR3β mediates butyrate -induced adipose lipolysis. In this study, weaned mice were were fed control (Con) or high-fat (HF) diet for 8 weeks to establish obesity. HF-induced obese mice maintained on the HF diet were divided into two sub-groups; HFB group was gavaged with 80 mg sodium butyrate (SB) per mouse every other day for 10 days, while the HF group received vehicle. Chromatin immunoprecipitation assay was performed to determine the status of histone H3 lysine 9 acetylation (H3K9Ac) on the promoter of AR3β gene in epididymal white adipose tissue...
December 28, 2016: Experimental Physiology
https://www.readbyqxmd.com/read/28025489/the-effects-of-ascorbate-n-acetylcysteine-and-resveratrol-on-fibroblasts-from-patients-with-mitochondrial-disorders
#9
Liza Douiev, Devorah Soiferman, Corinne Alban, Ann Saada
Reactive oxygen species (ROS) are assumed to be implicated in the pathogenesis of inborn mitochondrial diseases affecting oxidative phosphorylation (OXPHOS). In the current study, we characterized the effects of three small molecules with antioxidant properties (N-acetylcysteine, ascorbate, and resveratrol) on ROS production and several OXPHOS parameters (growth in glucose free medium, ATP production, mitochondrial content and membrane potential (MMP)), in primary fibroblasts derived from seven patients with different molecularly defined and undefined mitochondrial diseases...
December 22, 2016: Journal of Clinical Medicine
https://www.readbyqxmd.com/read/28008100/melatonin-protects-against-uric-acid-induced-mitochondrial-dysfunction-oxidative-stress-and-triglyceride-accumulation-in-c2c12-myotubes
#10
Gerald J Maarman, Brittany M Andrew, Dee M Blackhurst, Edward O Ojuka
Excess uric acid has been shown to induce oxidative stress, triglyceride accumulation and mitochondrial dysfunction in the liver and is an independent predictor of type-2 diabetes. Skeletal muscle plays a dominant role in type 2 diabetes and presents a large surface area to plasma uric acid. However, the effects of uric acid on skeletal muscle is underinvestigated. Our aim was therefore to characterize the effects of excessive uric acid on oxidative stress, triglyceride content and mitochondrial function in skeletal muscle C2C12 tubes and assess how these are modulated by the antioxidant molecule, melatonin...
December 22, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27991883/microbial-stimulation-of-different-toll-like-receptor-signalling-pathways-induces-diverse-metabolic-programmes-in-human-monocytes
#11
Ekta Lachmandas, Lily Boutens, Jacqueline M Ratter, Anneke Hijmans, Guido J Hooiveld, Leo A B Joosten, Richard J Rodenburg, Jack A M Fransen, Riekelt H Houtkooper, Reinout van Crevel, Mihai G Netea, Rinke Stienstra
Microbial stimuli such as lipopolysaccharide (LPS) induce robust metabolic rewiring in immune cells known as the Warburg effect. It is unknown whether this increase in glycolysis and decrease in oxidative phosphorylation (OXPHOS) is a general characteristic of monocytes that have encountered a pathogen. Using CD14(+) monocytes from healthy donors, we demonstrated that most microbial stimuli increased glycolysis, but that only stimulation of Toll-like receptor (TLR) 4 with LPS led to a decrease in OXPHOS. Instead, activation of other TLRs, such as TLR2 activation by Pam3CysSK4 (P3C), increased oxygen consumption and mitochondrial enzyme activity...
December 19, 2016: Nature Microbiology
https://www.readbyqxmd.com/read/27984028/large-scale-gene-expression-profiling-platform-for-identification-of-context-dependent-drug-responses-in-multicellular-tumor-spheroids
#12
Wojciech Senkowski, Malin Jarvius, Jenny Rubin, Johan Lengqvist, Mats G Gustafsson, Peter Nygren, Kim Kultima, Rolf Larsson, Mårten Fryknäs
Cancer cell lines grown as two-dimensional (2D) cultures have been an essential model for studying cancer biology and anticancer drug discovery. However, 2D cancer cell cultures have major limitations, as they do not closely mimic the heterogeneity and tissue context of in vivo tumors. Developing three-dimensional (3D) cell cultures, such as multicellular tumor spheroids, has the potential to address some of these limitations. Here, we combined a high-throughput gene expression profiling method with a tumor spheroid-based drug-screening assay to identify context-dependent treatment responses...
November 17, 2016: Cell Chemical Biology
https://www.readbyqxmd.com/read/27976130/manipulation-of-nos-activity-in-the-context-of-glucose-stress-alters-ampk-and-mitochondrial-oxphos-protein-content
#13
A C Keller, L A Knaub, P M McClatchey, C A Connon, Jeb Reusch
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27974213/reversal-of-defective-mitochondrial-biogenesis-in-limb-girdle-muscular-dystrophy-2d-by-independent-modulation-of-histone-and-pgc-1%C3%AE-acetylation
#14
Sarah Pambianco, Matteo Giovarelli, Cristiana Perrotta, Silvia Zecchini, Davide Cervia, Ilaria Di Renzo, Claudia Moscheni, Michela Ripolone, Raffaella Violano, Maurizio Moggio, Maria Teresa Bassi, Pier Lorenzo Puri, Lucia Latella, Emilio Clementi, Clara De Palma
Mitochondrial dysfunction occurs in many muscle degenerative disorders. Here, we demonstrate that mitochondrial biogenesis was impaired in limb-girdle muscular dystrophy (LGMD) 2D patients and mice and was associated with impaired OxPhos capacity. Two distinct approaches that modulated histones or peroxisome proliferator-activated receptor-gamma coactivator 1 α (PGC-1α) acetylation exerted equivalent functional effects by targeting different mitochondrial pathways (mitochondrial biogenesis or fatty acid oxidation[FAO])...
December 13, 2016: Cell Reports
https://www.readbyqxmd.com/read/27973917/mitochondrial-variations-in-the-mt-nd4-and-mt-tl1-genes-are-associated-with-male-infertility
#15
Feng Ni, Yun Zhou, Wen-Xiang Zhang, Xue-Mei Wang, Xiao-Min Song, Hong Jiang
: Mitochondrial gene mutations have been reported to be associated with sperm motility and the quality of semen. The aim of this study was to investigate whether the two mitochondrial genes (MT-ND4 and MT-TL1) are involved in Chinese male infertility. A total of 97 asthenospermia patients and 80 fertile controls were recruited in this case-control study. Genomic DNA were extracted from the sperm of all participants. Two mitochondrial DNA genes (MT-ND4 and MT-TL1) were amplified by using polymerase chain reaction (PCR) with the gene-specific primers and sequenced on an ABI 3730XL DNA sequencer...
December 14, 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27943495/the-mitochondrial-complexome-of-arabidopsis-thaliana
#16
Jennifer Senkler, Michael Senkler, Holger Eubel, Tatjana Hildebrandt, Christian Lengwenus, Peter Schertl, Markus Schwarzländer, Stephan Wagner, Ilka Wittig, Hans-Peter Braun
Mitochondria are central to cellular metabolism and energy conversion. In plants they also enable photosynthesis through additional components and functional flexibility. A majority of those processes rely on the assembly of individual proteins to larger protein complexes, some of which operate as large molecular machines. There has been a strong interest in the makeup and function of mitochondrial protein complexes and protein-protein interactions in plants, but the experimental approaches used typically suffer from selectivity or bias...
December 10, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27940153/fyn-kinase-regulates-translation-in-mammalian-mitochondria
#17
Emine C Koc, Jennifer L Miller-Lee, Hasan Koc
BACKGROUND: Mitochondrial translation machinery solely exists for the synthesis of 13 mitochondrially-encoded subunits of the oxidative phosphorylation (OXPHOS) complexes in mammals. Therefore, it plays a critical role in mitochondrial energy production. However, regulation of the mitochondrial translation machinery is still poorly understood. In comprehensive proteomics studies with normal and diseased tissues and cell lines, we and others have found the majority of mitochondrial ribosomal proteins (MRPs) to be phosphorylated...
December 7, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27939921/regulation-of-oxidative-phosphorylation-through-each-step-activation-esa-evidences-from-computer-modeling
#18
REVIEW
Bernard Korzeniewski
The mechanisms responsible for matching of the highly varying ATP demand by ATP supply in muscle are of primary importance for pure science, sport science and medicine. According to the traditional opinion ATP supply is activated by elevated ADP and Pi resulting from ATP hydrolysis during intensive work. Theoretical studies using the computer model of oxidative phosphorylation (OXPHOS) and the entire cell bioenergetic system developed by the author and co-workers lead to the each-step-activation (ESA) mechanism of the regulation of the system in skeletal muscle, heart and other tissues during work transitions...
December 8, 2016: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/27923774/mechanistic-insights-into-selective-killing-of-oxphos-dependent-cancer-cells-by-arctigenin
#19
Karin Brecht, Virginie Riebel, Philippe Couttet, Franziska Paech, Armin Wolf, Salah-Dine Chibout, Francois Pognan, Stephan Krähenbühl, Marianne Uteng
Arctigenin has previously been identified as a potential anti-tumor treatment for advanced pancreatic cancer. However, the mechanism of how arctigenin kills cancer cells is not fully understood. In the present work we studied the mechanism of toxicity by arctigenin in the human pancreatic cell line, Panc-1, with special emphasis on the mitochondria. A comparison of Panc-1 cells cultured in glucose versus galactose medium was applied, allowing assessments of effects in glycolytic versus oxidative phosphorylation (OXPHOS)-dependent Panc-1 cells...
December 5, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27922662/metabolic-reprogramming-and-ampk%C3%AE-1-pathway-activation-by-caulerpin-in-colorectal-cancer-cells
#20
Hua Yu, Huiqin Zhang, Mingjun Dong, Zhou Wu, Zhonglei Shen, Yangyang Xie, Zhenfang Kong, Xiaoyu Dai, Binbin Xu
Caulerpin, a secondary metabolite from the marine invasive green algae Caulerpa cylindracea is known to induce mitochondrial dysfunctions. In this study, the anticancer property of caulerpin was assessed in a panel of colorectal cancer cell lines. We demonstrated that caulerpin inhibited oxidative phosphorylation (OXPHOS) and facilitated an early intervention of the mitochondrial function, via inhibiting mitochondrial complex I, accompanied by the dissipation of mitochondrial membrane potential and a surge of reactive oxygen species (ROS) generation...
January 2017: International Journal of Oncology
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