keyword
MENU ▼
Read by QxMD icon Read
search

alternative oxidase

keyword
https://www.readbyqxmd.com/read/29156345/the-extended-production-of-uv-induced-reactive-oxygen-species-in-l929-fibroblasts-is-attenuated-by-posttreatment-with-arrabidaea-chica-through-scavenging-mechanisms
#1
Fabianne Martins Ribeiro, Hélito Volpato, Danielle Lazarin-Bidóia, Vânia Cristina Desoti, Rebeca Oliveira de Souza, Maria José Vieira Fonseca, Tânia Ueda-Nakamura, Celso Vataru Nakamura, Sueli de Oliveira Silva
Ultraviolet radiation (UVR) exposure causes various injurious effects to human skin by generating reactive oxygen species (ROS). Excessive ROS production can lead to oxidative stress which may damage cellular components like lipids and proteins and causing photoaging. The use of natural photochemopreventive agents with antioxidant properties is an important alternative to improve the effectiveness of sunscreens and reduce skin photodamage. A crude extract (CE) from the leaves of Arrabidaea chica underwent partition by a liquid-liquid method...
November 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29152973/epidermal-microfluidic-electrochemical-detection-system-enhanced-sweat-sampling-and-metabolite-detection
#2
Aida Martin, Jayoung Kim, Jonas F Kurniawan, Juliane R Sempionatto, Jose R Moreto, Guangda Tang, Alan C Campbell, Andrew Shin, Min Yul Lee, Xiaofeng Liu, Joseph Wang
Despite tremendous recent efforts, non-invasive sweat monitoring is still far from delivering its early analytical promise. Here, we describe a flexible epidermal microfluidic detection platform fabricated through hybridization of lithographic and screen-printed technologies for efficient sweat sampling and continuous real-time electrochemical monitoring of sweat glucose and lactate levels. This soft, skin-mounted device judiciously merges lab-on-a-chip and electrochemical detection technologies, integrated with a miniaturized flexible electronic board for real-time wireless data transmission to a smart device...
November 20, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29146253/isolation-and-characterisation-of-theobromine-degrading-filamentous-fungi
#3
Daniel Oduro-Mensah, Augustine Ocloo, Sammy T Lowor, Evelyn Y Bonney, Laud K N A Okine, Naa Ayikailey Adamafio
Strategies for achieving global food security include identification of alternative feedstock for use as animal feed, to contribute towards efforts at increasing livestock farming. The presence of theobromine in cocoa pod husks, a major agro-waste in cocoa-producing countries, hinders its utilisation for this purpose. Cheap treatment of cocoa pod husks to remove theobromine would allow largescale beneficial use of the millions of metric tonnes generated annually. The aim of this study was to isolate theobromine-degrading filamentous fungi that could serve as bioremediation agents for detheobromination of cocoa pod husks...
January 2018: Microbiological Research
https://www.readbyqxmd.com/read/29127553/identification-of-histidine-303-as-the-catalytic-base-of-lysyl-oxidase-via-site-directed-mutagenesis
#4
Rachel N Oldfield, Kathryn A Johnston, Jeanette Limones, Caitlin Ghilarducci, Karlo M Lopez
Lysyl oxidase (LOX) is a copper-dependent amine oxidase enzyme that catalyzes the formation of crosslinkages of collagen and elastin in connective tissues by oxidative deamination of lysine. Using site-directed mutagenesis, Histidine 303 has been shown to be a key residue that acts as the necessary catalytic base for this enzyme to function properly. Histidine 303 was mutated to isoleucine to remove catalytic activity and to aspartate and glutamate, respectively, in order to provide alternate residues that could act as a general base that could maintain catalytic activity...
November 10, 2017: Protein Journal
https://www.readbyqxmd.com/read/29113138/evaluating-the-potential-value-of-natural-product-cuminic-acid-against-plant-pathogenic-fungi-in-cucumber
#5
Yong Wang, Jie Zhang, Yang Sun, Juntao Feng, Xing Zhang
Fusarium wilt and anthracnose are two major diseases which limit the yield and quality of cucumber worldwide. Cuminic acid was extracted from the seed of Cuminum cyminum L. The mean EC50 values of cuminic acid for inhibiting mycelial growth and zoospore germination of five Fusarium oxysporum f. sp. cucumerinum strains were 25.66 ± 3.02 μg/mL and 15.99 ± 2.19 μg/mL, and of four Colletotrichum lagenarium (Pass.) Ellis and Halsted strains were 29.53 ± 3.18 μg/mL and 18.41 ± 2.78 μg/mL, respectively. In greenhouse experiments, cuminic acid at 2000 μg/mL exhibited 70...
November 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29112947/sugar-analog-synthesis-by-in-vitro-biocatalytic-cascade-a-comparison-of-alternative-enzyme-complements-for-dihydroxyacetone-phosphate-production-as-a-precursor-to-rare-chiral-sugar-synthesis
#6
Carol J Hartley, Nigel G French, Judith A Scoble, Charlotte C Williams, Quentin I Churches, Andrew R Frazer, Matthew C Taylor, Greg Coia, Gregory Simpson, Nicholas J Turner, Colin Scott
Carbon-carbon bond formation is one of the most challenging reactions in synthetic organic chemistry, and aldol reactions catalysed by dihydroxyacetone phosphate-dependent aldolases provide a powerful biocatalytic tool for combining C-C bond formation with the generation of two new stereo-centres, with access to all four possible stereoisomers of a compound. Dihydroxyacetone phosphate (DHAP) is unstable so the provision of DHAP for DHAP-dependent aldolases in biocatalytic processes remains complicated. Our research has investigated the efficiency of several different enzymatic cascades for the conversion of glycerol to DHAP, including characterising new candidate enzymes for some of the reaction steps...
2017: PloS One
https://www.readbyqxmd.com/read/29107420/african-trypanosomiasis-synthesis-sar-enabling-novel-drug-discovery-of-ubiquinol-mimics-for-trypanosome-alternative-oxidase
#7
Ryan A West, Oran G O'Doherty, Trevor Askwith, John Atack, Paul Beswick, Jamie Laverick, Michael Paradowski, Lewis E Pennicott, Srinivasa P S Rao, Gareth Williams, Simon E Ward
African trypanosomiasis is a parasitic disease affecting 5000 humans and millions of livestock animals in sub-Saharan Africa every year. Current treatments are limited, difficult to administer and often toxic causing long term injury or death in many patients. Trypanosome alternative oxidase is a parasite specific enzyme whose inhibition by the natural product ascofuranone (AF) has been shown to be curative in murine models. Until now synthetic methods to AF analogues have been limited, this has restricted both understanding of the key structural features required for binding and also how this chemotype could be developed to an effective therapeutic agent...
December 1, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29096084/nitric-oxide-upregulates-expression-of-alternative-oxidase-1-in-chlamydomonas-reinhardtii
#8
Zhanneta Zalutskaya, Mariya Ostroukhova, Valentina Filina, Elena Ermilova
The mitochondrial respiratory chain in plants, many fungi and some protists consists of the ATP-coupling cyanide-sensitive cytochrome pathway and the cyanide-resistant alternative respiratory pathway. The alternative pathway is mediated by alternative oxidase (AOX). In unicellular algae, AOXs are monomeric fungi-type proteins. Studies performed in the model plant Chlamydomonas reinhardtii showed that a range of stress factors lead to induction of its AOX1. However, signaling molecules that trigger upregulation of AOX1 have not yet been identified...
October 28, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29080105/a-modified-method-for-molecular-identification-of-baylisascaris-transfuga-in-european-brown-bears-ursus-arctos
#9
Jakub Gawor, Jan Gawor, Robert Gromadka, Tomasz Zwijacz-Kozica, Filip Zięba
Baylisascaris transfuga is a roundworm that has been reported worldwide in most bear species. In mammals and possibly humans, the larvae of B. transfuga can migrate in the tissues of aberrant hosts with larva migrans syndrome. The current study was performed to identify B. transfuga in faecal samples from free-ranging brown bears in the Tatra Mountains National Park in southern Poland. A commercial kit was used to extract genomic DNA directly from faecal samples. Additionally, a Chelex resin-based technique was successfully implemented to prepare a PCR template from eggs retrieved by flotation...
October 27, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29071435/the-effect-of-xanthine-oxidase-inhibitors-on-blood-pressure-and-renal-function
#10
REVIEW
Marilisa Bove, Arrigo F G Cicero, Claudio Borghi
Several epidemiological studies have demonstrated the existence of a correlation between high serum uric acid (SUA) levels, hypertension, and chronic kidney disease (CKD). Xantine oxidase inhibitors (XOI) are the most powerful uric acid lowering drugs, with presumed beneficial effects on cardiovascular and renal system. The multifactorial mechanism linking hyperuricemia with cardiovascular and renal diseases involves both the SUA level and the xanthine oxidase (XO) activity. In this context, the clinical research has been recently focused at assessing the efficacy of urate-lowering drugs active on XO in patients with abnormal blood pressure values and renal dysfunction...
October 25, 2017: Current Hypertension Reports
https://www.readbyqxmd.com/read/29069398/metabarcoding-analyses-enable-differentiation-of-both-interspecific-assemblages-and-intraspecific-divergence-in-habitats-with-differing-management-practices
#11
Pedro M Pedro, Ross Piper, Pedro Bazilli Neto, Laury Cullen, Milena Dropa, Rogerio Lorencao, Maria Helena Matté, Tatiane Cristina Rech, Mauro Osmar Rufato, Miriam Silva, Daniele Turganti Turati
Spatial and temporal collections provide important data on the distribution and dispersal of species. Regional-scale monitoring invariably involves hundreds of thousands of samples, the identification of which is costly in both time and money. In this respect, metabarcoding is increasingly seen as a viable alternative to traditional morphological identification, as it eliminates the taxonomic bottleneck previously impeding such work. Here, we assess whether terrestrial arthropods collected from 12 pitfall traps in two farms of a coffee ( Coffea arabica L...
October 24, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/29065339/reduced-graphene-oxide-bifeo3-nanohybrids-based-signal-on-photoelectrochemical-sensing-system-for-prostate-specific-antigen-detection-coupling-with-magnetic-microfluidic-device
#12
Qian Zhou, Youxiu Lin, Kangyao Zhang, Meijin Li, Dianping Tang
A novel magnetic controlled photoelectrochemical (PEC) sensing system was designed for sensitive detection of prostate-specific antigen (PSA) using reduced graphene oxide-functionalized BiFeO3 (rGO-BiFeO3) as the photoactive material and target-triggered hybridization chain reaction (HCR) for signal amplification. Remarkably enhanced PEC performance could be obtained by using rGO-BiFeO3 as the photoelectrode material due to its accelerated charge transfer and improved the visible light absorption. Additionally, efficient and simple operation could be achieved by introducing magnetic controlled flow-through device...
October 16, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29052944/differential-expression-of-the-three-alcanivorax-borkumensis-sk2-genes-coding-for-the-p450-cytochromes-involved-in-the-assimilation-of-hydrocarbons
#13
Emma Sevilla, Luis Yuste, Renata Moreno, Fernando Rojo
Alcanivorax borkumensis, a marine bacterium highly specialized in degrading linear and branched alkanes, plays a key ecological role in the removal of marine oil spills. It contains several alternative enzyme systems for terminal hydroxylation of alkanes, including three P450 cytochromes (P450-1, P450-2 and P450-3). The present work shows cytochrome P450-1 to be expressed from the promoter of the upstream gene fdx. Promoter Pfdx was more active when C8 -C18 n-alkanes or pristane were assimilated than when pyruvate was available...
October 20, 2017: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/29040900/alleviation-of-cr-vi-induced-oxidative-stress-in-maize-zea-mays-l-seedlings-by-no-and-h2s-donors-through-differential-organ-dependent-regulation-of-ros-and-nadph-recycling-metabolisms
#14
Oussama Kharbech, Hayet Houmani, Abdelilah Chaoui, Francisco J Corpas
Chromium (Cr) contamination in soil is a growing concern in relation to sustainable agricultural production and food safety. Nitric oxide (NO) and, more recently, hydrogen sulfide (H2S) are considered to be new signalling molecules with biotechnological applications in the agronomical sector. Using 9-day-old maize (Zea mays) seedlings exposed to 200μM Cr(VI), the potential mitigating effects of exogenous NO and H2S on chromium-induced stress in maize seedlings were investigated in roots, cotyledons and coleoptiles...
October 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29027090/morphological-and-molecular-data-for-a-new-species-of-pomphorhynchus-monticelli-1905-acanthocephala-pomphorhynchidae-in-the-mexican-redhorse-moxostoma-austrinum-bean-cypriniformes-catostomidae-in-central-mexico
#15
Martín García-Varela, Berenit Mendoza-Garfias, Anindo Choudhury, Gerardo Pérez-Ponce de León
Pomphorhynchus purhepechus n. sp. is described from the intestine of the Mexican redhorse Moxostoma austrinum Bean (Catostomidae) in central Mexico. The new species can be distinguished from the other seven described species of Pomphorhynchus Monticelli, 1905 in the Americas by a subspherical proboscis and 14 longitudinal rows with 16-18 hooks each; the third and the fourth row of hooks are alternately longest. Sequences of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene and the large subunit (LSU) rDNA (including the domains D2-D3) were used to corroborate the morphological distinction between the new species and Pomphorhynchus bulbocolli Linkins in Van Cleave, 1919, a species widely distributed in several freshwater fish species across Canada, USA, and Mexico...
November 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28987480/egg-white-derived-peptides-prevent-cardiovascular-disorders-induced-by-mercury-in-rats-role-of-angiotensin-converting-enzyme-ace-and-nadph-oxidase
#16
Danize Aparecida Rizzetti, Ángela Martín, Patricia Corrales, Francisca Fernandez, Maylla Ronacher Simões, Franck Maciel Peçanha, Dalton Valentim Vassallo, Marta Miguel, Giulia Alessandra Wiggers
The study aimed to investigate the effects of egg white hydrolysate (EWH) on vascular disorders induced by mercury (Hg). For this, male Wistar rats were treated for 60days: Untreated (saline, i.m.); Mercury (HgCl2, i.m., 1st dose 4.6μg/kg, subsequent doses 0.07μg/kg/day); Hydrolysate (EWH, gavage, 1g/kg/day); Hydrolysate-Mercury. Systolic (SBP) and diastolic (DBP) blood pressure measurement and vascular reactivity experiments in aorta were performed. We analyzed endothelial dependent and independent vasodilator responses and vasoconstrictor response to phenylephrine (Phe) in absence and presence of endothelium, a NOS inhibitor, a NADPH oxidase inhibitor, the superoxide dismutase, a non-selective COX inhibitor, a selective COX-2 inhibitor, an AT-1 receptors blocker...
November 5, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28981788/empty-pericarp11-serves-as-a-factor-for-splicing-of-mitochondrial-nad1-intron-and-is-required-to-ensure-proper-seed-development-in-maize
#17
Xuemei Ren, Zhenyuan Pan, Hailiang Zhao, Junli Zhao, Manjun Cai, Jiang Li, Zuxin Zhang, Fazhan Qiu
Group II introns are common in the mitochondrial genome of higher plant species. The splicing of these introns is a complex process involving the synergistic action of multiple factors. However, few of these factors have been characterized in maize. In this study, we found that the Empty pericarp11 (Emp11) gene, which encodes a P-type pentatricopeptide repeat (PPR) protein, is required for the development of maize seeds. The loss of Emp11 function seriously impairs embryo and endosperm development, resulting in empty pericarp seeds in maize, and alteration in Emp11 expression leads to quantitative variation in kernel size and weight...
July 20, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28965311/dna-barcoding-for-identification-of-sugarcane-borers-in-china
#18
J- D Wang, W- Z Wang, Z- L Lin, A Ali, H- Y Fu, M- T Huang, S- J Gao, R Wang
Sugarcane borers are economically damaging insects with species that vary in distribution patterns both geographically and temporally, and vary based on ecological niche. Currently, identification of sugarcane borers is mostly based on morphological characters. However, morphological identification requires taxonomic expertise. An alternative method to identify sugarcane borers is the use of molecular data. DNA barcoding based on partial cytochrome c oxidase subunit 1 (COI) sequences has proven to be a useful tool for rapid and accurate species determination in many insect taxa...
September 30, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28961963/glycerol-metabolism-and-its-implication-in-virulence-in-mycoplasma
#19
Cedric Blötz, Jörg Stülke
Glycerol and glycerol-containing compounds such as lipids belong to the most abundant organic compounds that may serve as nutrient for many bacteria. For the cell wall-less bacteria of the genus Mycoplasma, glycerol derived from phospholipids of their human or animal hosts is the major source of carbon and energy. The lipids are first degraded by lipases, and the resulting glycerophosphodiesters are transported into the cell and cleaved to release glycerol-3-phosphate. Alternatively, free glycerol can be transported, and then become phosphorylated...
September 1, 2017: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/28959420/-on-demand-redox-buffering-by-h2s-contributes-to-antibiotic-resistance-revealed-by-a-bacteria-specific-h2s-donor
#20
Prashant Shukla, Vinayak S Khodade, Mallojjala SharathChandra, Preeti Chauhan, Saurabh Mishra, Shivakumara Siddaramappa, Bulagonda Eswarappa Pradeep, Amit Singh, Harinath Chakrapani
Understanding the mechanisms of antimicrobial resistance (AMR) will help launch a counter-offensive against human pathogens that threaten our ability to effectively treat common infections. Herein, we report bis(4-nitrobenzyl)sulfanes, which are activated by a bacterial enzyme to produce hydrogen sulfide (H2S) gas. We found that H2S helps maintain redox homeostasis and protects bacteria against antibiotic-triggered oxidative stress "on demand", through activation of alternate respiratory oxidases and cellular antioxidants...
July 1, 2017: Chemical Science
keyword
keyword
47891
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"